CN108934770A - A kind of pleurotus cornucopiae high-yield culture medium and pleurotus cornucopiae cultural method - Google Patents

A kind of pleurotus cornucopiae high-yield culture medium and pleurotus cornucopiae cultural method Download PDF

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Publication number
CN108934770A
CN108934770A CN201810700921.7A CN201810700921A CN108934770A CN 108934770 A CN108934770 A CN 108934770A CN 201810700921 A CN201810700921 A CN 201810700921A CN 108934770 A CN108934770 A CN 108934770A
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China
Prior art keywords
pleurotus cornucopiae
bag
culture medium
cultural
parts
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CN201810700921.7A
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Chinese (zh)
Inventor
范小林
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Leshan Hongkou District Hongxiang Mushroom Industry Co Ltd
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Leshan Hongkou District Hongxiang Mushroom Industry Co Ltd
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Priority to CN201810700921.7A priority Critical patent/CN108934770A/en
Publication of CN108934770A publication Critical patent/CN108934770A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

Abstract

Pleurotus cornucopiae high-yield culture medium of the present invention, it is made of following components proportion: wheat bran 20-30, sawdust 50-80, sawdust 40-50, corncob 10-30, cornstalk 10-30, straw 10-30, lima bean shell 10-20,5-8 parts of sorghum flour, 8-12 parts of soybean powder, quick lime 1-10, gypsum 1-10,1-5 parts of calcium dihydrogen phosphate, 1-5 parts of sucrose, moisture content in medium is 60-70%, pH 4-8.5.The present invention also provides the cultural methods based on above-mentioned culture medium.Cultural method is combined on the basis of the culture medium, and high yield is obtained while start-up cost is cheap.

Description

A kind of pleurotus cornucopiae high-yield culture medium and pleurotus cornucopiae cultural method
Technical field
The invention belongs to planting edible mushroom fields, and in particular to pleurotus cornucopiae culture medium and cultural method.
Background technique
Pleurotus cornucopiae is unique oyster mushroom type, occupies the 10th in the sequence of China's edible mushroom type yield, is China's cultivation Important type in edible mushroom.It is deep due to its is soft palatable, full of nutrition, moderate to be liked by the majority of consumers, it is hundred Common cuisines on surname dining table have good sales volume and higher economic benefit.Pleurotus cornucopiae contains the selenium of antitumor cell, polysaccharide The substances such as body have very strong inhibiting effect to tumour cell, and have immunological characteristic;The multivitamin and mine that pleurotus cornucopiae contains Substance can improve human metabolism, build up health, adjust the effects of vegetative nerve function, therefore can be used as weak patient's Nutriment, it is all effective in cure to hepatitis, chronic gastritis, gastric duodenal ulcer, osteomalacia, hypertension etc., it is solid to blood gallbladder is reduced Pure and mild prevention and treatment urethral calculus also has certain effect.
Pleurotus cornucopiae is to contain cellulose, hemicellulose and wood by saprophytic or parasitic mode as all edible mushrooms It is grown on the organic matter of quality, is converted into edible protein, therefore, yield, quality plant compost used with it It is closely bound up.Traditional pleurotus cornucopiae culturing raw material is from a wealth of sources, and pleurotus cornucopiae mycelia decomposes very competent, the general agriculture of lignin, cellulose Crop straw all can be used as the compost of cultivation pleurotus cornucopiae, be suitable for promoting, but yield is lower simultaneously, higher cost, so that large area Popularization has certain limitation.Searching is more suitable for pleurotus cornucopiae growth and culture medium at low cost, that yield is high, while improving pleurotus cornucopiae battalion Feeding value is of great significance.
Summary of the invention
The purpose of the present invention is to provide a kind of pleurotus cornucopiae high-yield culture medium and pleurotus cornucopiae cultural methods, on the basis of the culture medium Upper combination cultural method, obtains high yield while cost of implementation is cheap.
Pleurotus cornucopiae high-yield culture medium of the present invention is made of: wheat bran 20-30, sawdust 50-80, sawdust following components proportion 40-50, corncob 10-30, cornstalk 10-30, straw 10-30, lima bean shell 10-20,5-8 parts of sorghum flour, 8-12 parts of soybean powder, Quick lime 1-10, gypsum 1-10,1-5 parts of calcium dihydrogen phosphate, 1-5 parts of sucrose, moisture content in medium 60-70%, pH 4- 8.5。
Further, the medium pH is 6.5-7.5.
Based on the pleurotus cornucopiae cultural method of the pleurotus cornucopiae high-yield culture medium, steps are as follows:
(1) cornstalk, straw, lima bean shell are crushed, adds water to mix thoroughly with other solid components in formula, control water content 60- 70%(mass percent), pH 4-8.5 obtains compost;
(2) gained compost is packed into polybag, the specification of plastic bag is diameter 20-25cm, length 30-35cm, will be described One end of polybag fraps, and is packed into the compost compacting, and leveling is sturdy after having charged, and fraps the other end, obtains Bacterium bag;
(3) bacterium bag is put into woven bag, is then placed into autoclave;When sterilising temp reaches 100 DEG C, 8-10h is kept Stop heating afterwards, the bacterium bag after keeping 12h, after taking out sterilizing;
(4) when the bacterium bag after sterilizing being cooled to 10-30 DEG C, sterile working is carried out, strain is accessed at bag body both ends, by the bacterium Kind uniform fold surface, strain dosage are the 4%~6% of compost, cover the fruiting that upper diameter is 4~6cm in sack after inoculation Circle, and sealed with aseptic paper to get the cultivating bag to after being inoculated with;
(5) cultivating bag after inoculation is put into the bacteria-producing room culture of dark environment, during which temperature and bacterium bag bacterium between running check bag Silk upgrowth situation, at 5-30 DEG C, moisture content in medium is controlled in 60%-70% control mycelial growth temperature, is maintained the circulation of air;
(6) after the long purseful of pleurotus cornucopiae mycelia, when bacteria-producing room places 7~10d again, reaches physiological maturity, that is, bacteria-producing room, code be may move into Bag wall fruiting, controls 10-18 DEG C of sporophore growth temperature, thallus humidity 60%-80%.
(7) when the cap of pleurotus cornucopiae is unfolded, mushroom body colour is shallow, and lid edge is thinning, i.e., will harvest before dissipating spore.
Further, control mycelial growth temperature is 20-25 DEG C.
Further, control sporophore growth temperature is 13-18 DEG C.
Further, medium pH 6.5-7.5 can be regulated and controled by acetic acid.
Compared with prior art, the present invention has the effect that
1, culture medium of the present invention has been especially added with sorghum flour, soybean powder, lima bean shell, is conducive to improve and enrich pleurotus cornucopiae battalion It forms point, and yield can be improved.
2, pleurotus cornucopiae training base component of the present invention is old cheap material, low ensureing in conjunction with the cultural method Under conditions of cost and economy, the quality of the pleurotus cornucopiae of output is improved.
Specific embodiment
Below by specific embodiment, pleurotus cornucopiae high-yield culture medium of the present invention and pleurotus cornucopiae cultural method are done into one Walk explanation.
Embodiment 1
Pleurotus cornucopiae high-yield culture medium, by following components proportion form: wheat bran 20, sawdust 80, sawdust 40, corncob 30, cornstalk 10, Straw 130, lima bean shell 10,8 parts of sorghum flour, 8 parts of soybean powder, quick lime 10, gypsum 1,5 parts of calcium dihydrogen phosphate, 1 part of sucrose, training Supporting base water content is 60%, pH 6.5.
Based on the pleurotus cornucopiae cultural method of the above pleurotus cornucopiae high-yield culture medium, steps are as follows:
(1) cornstalk, straw, lima bean shell are crushed, adds water to mix thoroughly with other solid components in formula, control water content 70%, pH 6.5 obtains compost;
(2) gained compost is packed into polybag, the specification of plastic bag is diameter 20cm, length 55cm, by the polybag One end frap, be packed into compost compacting, charge after leveling in a down-to-earth manner, frap the other end, obtain bacterium bag;
(3) bacterium bag for installing culture medium is put into woven bag, is then placed into autoclave;When sterilising temp reaches 100 DEG C When, stop heating after keeping 8-h, the bacterium bag after keeping 12h, after taking out sterilizing;
(4) it when the bacterium bag after sterilizing being cooled to 20 DEG C, carries out sterile working: strain is accessed at bacterium bag both ends, by the strain Two end surfaces of uniform fold, strain dosage are the 4% of compost, cover the fruiting circle that upper diameter is 6cm, sack in sack after inoculation It is sealed with aseptic paper to get the cultivating bag to after being inoculated at both ends;
(5) cultivating bag after inoculation is put into the bacteria-producing room culture of dark environment, during which temperature and bacterium bag bacterium between running check bag Silk upgrowth situation, at 25 DEG C, moisture content in medium is controlled 70% control mycelial growth temperature, is maintained the circulation of air;
(6) after the long purseful of pleurotus cornucopiae mycelia, when bacteria-producing room places 7d again, reaches physiological maturity, that is, bacteria-producing room, code bag wall be may move into Formula fruiting controls 18 DEG C of sporophore growth temperature, thallus humidity 80%%.
(7) when the cap of pleurotus cornucopiae is unfolded, mushroom body colour is shallow, and lid edge is thinning, i.e., will harvest before dissipating spore.
Embodiment 2
Pleurotus cornucopiae high-yield culture medium, by following components proportion form: wheat bran 30, sawdust 50, sawdust 50, corncob 10, cornstalk 30, Straw 10, lima bean shell 20,5 parts of sorghum flour, 12 parts of soybean powder, quick lime 1, gypsum 10,1 part of calcium dihydrogen phosphate, 5 parts of sucrose, training Supporting base water content is 70%, pH 8.
Based on the pleurotus cornucopiae cultural method of the pleurotus cornucopiae high-yield culture medium, steps are as follows:
(1) cornstalk, straw, lima bean shell are crushed, add water to mix thoroughly with other solid components in formula, control water content 70%, PH is 8, obtains compost;
(2) gained compost is packed into polybag, the specification of plastic bag is diameter 30cm, length 40cm, by the one of polybag End fraps, and is packed into compost compacting, and leveling is sturdy after having charged, and fraps the other end, obtains bacterium bag;
(3) bacterium bag for installing culture medium is put into woven bag, is then placed into autoclave;When sterilising temp reaches 100 DEG C When, stop heating after keeping 8h, the bacterium bag after keeping 12h, after taking out sterilizing;
(4) it when the bacterium bag after sterilizing being cooled to 30 DEG C, carries out sterile working: accessing strain at the sterilizing material bag both ends, it will Sterilize material bag surface described in the strain uniform fold, and the strain dosage is the 6% of compost, upper straight in sack set after inoculation Diameter is the fruiting circle of 4cm, and the sack both ends are sealed with aseptic paper to get the cultivating bag to after being inoculated with;
(5) cultivating bag after inoculation is put into the bacteria-producing room culture of dark environment, during which temperature and bacterium bag bacterium between running check bag Silk upgrowth situation, at 25 DEG C, moisture content in medium is controlled in 60%-70% control mycelial growth temperature, is maintained the circulation of air;
(6) after the long purseful of pleurotus cornucopiae mycelia, when bacteria-producing room places 7~10d again, reaches physiological maturity, that is, bacteria-producing room, code be may move into Bag wall fruiting, controls 13 DEG C of sporophore growth temperature, thallus humidity 70%.
(7) when the cap of pleurotus cornucopiae is unfolded, mushroom body colour is shallow, and lid edge is thinning, i.e., will harvest before dissipating spore.

Claims (6)

1. a kind of pleurotus cornucopiae high-yield culture medium is made of: wheat bran 20-30, sawdust 50-80, sawdust 40-50, jade following components proportion Rice core 10-30, cornstalk 10-30, straw 10-30, lima bean shell 10-20,5-8 parts of sorghum flour, 8-12 parts of soybean powder, quick lime 1- 10, gypsum 1-10,1-5 parts of calcium dihydrogen phosphate, 1-5 parts of sucrose, moisture content in medium 60-70%, pH 4-8.5.
2. pleurotus cornucopiae high-yield culture medium according to claim 1, it is characterised in that the pH of culture medium is 6.5-7.5.
3. the pleurotus cornucopiae cultural method based on claim 1 based on the pleurotus cornucopiae high-yield culture medium, it is characterised in that steps are as follows:
(1) cornstalk, straw, lima bean shell are crushed, adds water to mix thoroughly with other solid components in formula, control water content 60- 70%(mass percent), pH 4-8.5 obtains compost;
(2) gained compost is packed into polybag, the specification of plastic bag is diameter 20-25cm, length 30-35cm, will be described One end of polybag fraps, and is packed into the compost compacting, and leveling is sturdy after having charged, and fraps the other end, obtains Bacterium bag;
(3) bacterium bag is put into woven bag, is then placed into autoclave;When sterilising temp reaches 100 DEG C, 8-10h is kept Stop heating afterwards, the bacterium bag after keeping 12h, after taking out sterilizing;
(4) when the bacterium bag after sterilizing being cooled to 10-30 DEG C, sterile working is carried out, strain is accessed at bag body both ends, by the bacterium Kind uniform fold surface, strain dosage are the 4%~6% of compost, cover the fruiting that upper diameter is 4~6cm in sack after inoculation Circle, and sealed with aseptic paper to get the cultivating bag to after being inoculated with;
(5) cultivating bag after inoculation is put into the bacteria-producing room culture of dark environment, during which temperature and bacterium bag bacterium between running check bag Silk upgrowth situation, at 5-30 DEG C, moisture content in medium is controlled in 60%-70% control mycelial growth temperature, is maintained the circulation of air;
(6) after the long purseful of pleurotus cornucopiae mycelia, when bacteria-producing room places 7~10d again, reaches physiological maturity, that is, bacteria-producing room, code be may move into Bag wall fruiting, controls 10-18 DEG C of sporophore growth temperature, thallus humidity 80%-90%;
(7) when the cap of pleurotus cornucopiae is unfolded, mushroom body colour is shallow, and lid edge is thinning, i.e., will harvest before dissipating spore.
4. pleurotus cornucopiae cultural method according to claim 3, it is characterised in that control mycelial growth temperature is 20-25 DEG C.
5. pleurotus cornucopiae cultural method according to claim 3, it is characterised in that control sporophore growth temperature is 13-18 DEG C.
6. pleurotus cornucopiae cultural method according to claim 3, it is characterised in that control medium pH is 6.5-7.5, passes through acetic acid Regulation.
CN201810700921.7A 2018-06-29 2018-06-29 A kind of pleurotus cornucopiae high-yield culture medium and pleurotus cornucopiae cultural method Pending CN108934770A (en)

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CN111296180A (en) * 2020-04-15 2020-06-19 贵州妙之农农业开发有限公司 High-quality and high-yield cultivation method for pleurotus cornucopiae planted by pine and cedar wood chips

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CN111296180A (en) * 2020-04-15 2020-06-19 贵州妙之农农业开发有限公司 High-quality and high-yield cultivation method for pleurotus cornucopiae planted by pine and cedar wood chips

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