CN108931405B

CN108931405B - Cell pelleter is with making piece holder

Info

Publication number: CN108931405B
Application number: CN201710370191.4A
Authority: CN
Inventors: 夏炜
Original assignee: Beijing Chengzhi Guanghui Tech Co ltd
Current assignee: Beijing Chengzhi Guanghui Tech Co ltd
Priority date: 2017-05-23
Filing date: 2017-05-23
Publication date: 2020-12-11
Anticipated expiration: 2037-05-23
Also published as: CN108931405A

Abstract

The invention discloses a slide making clamp for a cell slide making machine, which has the technical scheme that the slide making clamp comprises a box body for clamping a slide glass, a liquid storage hopper for storing sample liquid and a flow guide pipe, wherein the box body comprises a front side plate, a rear side plate and a bottom plate for connecting the front side plate and the rear side plate, a painting hole is formed in the position, corresponding to the slide glass, on the front side plate, one end of the flow guide pipe is communicated with the painting hole, the other end of the flow guide pipe is communicated with the liquid storage hopper, the slide making clamp also comprises a lifting part in sliding fit with the box body, the lifting part comprises a vertical pull plate and a supporting plate fixedly connected to the lower end of the vertical pull plate, one side of the vertical pull plate is attached to the rear side plate, and one. The invention solves the problem that the water-absorbing paper is easy to be retained in the film making clamp in the use process of the existing film making clamp.

Description

Cell pelleter is with making piece holder

Technical Field

The invention relates to liquid-based thin-layer cell detection, in particular to a slide making clamp for a cell slide making machine.

Background

The liquid-based thin-layer cell detection method comprises the following steps: sampling by using a sampling brush, rinsing the sampling brush by using a preserving fluid and preserving the sample, shaking and dissolving, flaking liquid-based thin-layer cells and observing by using a microscope. In the prior art, smear preparation is usually carried out manually, when a specimen is prepared manually, exfoliated cells on a glass slide are difficult to coat uniformly, and in addition, due to the fact that the specimen for sampling the exfoliated cells contains mucus, red blood cells and other impurities, the background of a specimen smear is not clean and clear, so that the observation analysis and diagnosis of a pathologist are influenced.

The utility model discloses a chinese utility model patent of grant bulletin number CN202471474U has announced a cell slide making machine is with system slide holder is used for solving above-mentioned problem, this system slide holder uses and compares conveniently, but this system slide making is pressed from both sides in the in-service use, the paper that absorbs wherein will absorb and surpass its inner ring regional unnecessary cell and liquid, because there is the opening and will absorb a large amount of liquid simultaneously, so the paper that absorbs water can become comparatively soft easily broken, when follow-up staff will take out slide glass and paper that absorbs water together, the paper that absorbs water can be detained in system slide holder the inside, bring the trouble for follow-up work.

Disclosure of Invention

The invention aims to provide a slide making clamp for a cell slide maker, which enables a worker to conveniently take out a glass slide and absorbent paper together.

The above object of the present invention is achieved by the following technical solutions: the utility model provides a cell slide maker is with system piece clamp, is including the box body that is used for the clamping slide glass, the stock solution fill and the honeycomb duct that is used for storing the appearance liquid, and the box body includes preceding curb plate, posterior lateral plate and is used for connecting the bottom plate of preceding curb plate and posterior lateral plate, the hole of dying has been seted up to the position department that corresponds the slide glass on the preceding curb plate, honeycomb duct one end intercommunication is painted hole and other end intercommunication liquid storage hopper, system piece clamp is still drawn the piece including the carrying of the cooperation box body that slides, carry and draw the piece and include vertical arm-tie and fixed connection in the layer board of vertical arm-tie lower extreme, one side laminating posterior lateral plate of vertical arm-tie, curb plate before the layer board deviates from.

Through adopting above-mentioned technical scheme, during the use, earlier with carrying in the pull-up piece vertical insertion box body, at this moment vertical arm-tie one side laminating posterior lateral plate, the bottom laminating box body bottom plate of layer board. The upper surface of the supporting plate is in a horizontal state, and one side of the supporting plate, which deviates from the vertical pulling plate, is attached to the front side plate. Then, the glass slide and the absorbent paper are inserted, and the lower edges of the glass slide and the absorbent paper are supported on the supporting plate. And then, starting centrifugal motion, wherein the sample liquid is jetted to the glass slide from the opening of the liquid storage hopper through the flow guide pipe during the centrifugal motion, and the sample liquid is scattered on the surface of the glass slide under the action of collision force to form a thin adhesion layer. After the slide is finished, the lifting piece, the slide glass and the absorbent paper are simultaneously pinched by hands and then lifted upwards, and at the moment, the supporting plate can hold the absorbent paper upwards to separate from the box body, so that the absorbent paper cannot be retained in the slide holder.

The invention is further configured to: the liquid storage bucket is composed of a hemispherical bucket bottom and a horn mouth connected above the bucket bottom, and the bottom of the bucket bottom is lower than the lower end of the flow guide pipe.

By adopting the technical scheme, the horn mouth can enable a worker to conveniently drop or pour the sample liquid into the liquid storage hopper; meanwhile, the hemispherical bucket bottom can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide after being dyed is further ensured.

The invention is further configured to: one or more through air holes are formed in the rear side plate.

Through adopting above-mentioned technical scheme to make the film-making press from both sides when the centrifugation film-making, the gas between slide glass and the box body can discharge fast, makes the effective posterior lateral plate of laminating the box body of slide glass, and then makes the effective area of painting and dyeing that the appearance liquid can effectively adhere to the slide glass.

The invention is further configured to: be provided with the filter screen that can dismantle the connection in the liquid storage fill, the filter screen is the pocket form, the last border of filter screen is taken and is put on the last border of liquid storage fill and the below of filter screen stretches into in the liquid storage fill.

By adopting the technical scheme, the sample solution contains impurities such as mucus, red blood cells and the like, and the impurities can influence the definition of the stained part of the glass slide, so the filter screen is arranged in the liquid storage hopper. The diameter of the mesh of the filter screen is about 6 microns, mucus and red blood cells can be filtered, and then the development of the stained glass slide is clearer.

The invention is further configured to: the upper edge of the rear side plate is provided with a downward sunken U-shaped notch, and the upper edge of the front side plate is lower than or equal to the height of the bottom of the U-shaped notch.

By adopting the technical scheme, the glass slide is conveniently pinched and taken out from the box body by the working personnel.

In conclusion, the invention has the following beneficial effects: when the lifting plate is used, the lifting piece is vertically inserted into the box body, one side of the vertical pulling plate is attached to the rear side plate, and the bottom of the supporting plate is attached to the bottom plate of the box body. The upper surface of the supporting plate is in a horizontal state, and one side of the supporting plate, which deviates from the vertical pulling plate, is attached to the front side plate. Then, the glass slide and the absorbent paper are inserted, and the lower edges of the glass slide and the absorbent paper are supported on the supporting plate. And then, starting centrifugal motion, wherein the sample liquid is jetted to the glass slide from the opening of the liquid storage hopper through the flow guide pipe during the centrifugal motion, and the sample liquid is scattered on the surface of the glass slide under the action of collision force to form a thin adhesion layer. After the slide is finished, the lifting piece, the slide glass and the absorbent paper are simultaneously pinched by hands and then lifted upwards, and at the moment, the supporting plate can hold the absorbent paper upwards to separate from the box body, so that the absorbent paper cannot be retained in the slide holder.

Drawings

FIG. 1 is an isometric view of a slide holder;

FIG. 2 is a view for showing the structure of the airing hole;

FIG. 3 is a cross-sectional view of a film clip.

In the figure, 1, a box body; 10. a front side plate; 100. coating and dyeing holes; 11. a rear side plate; 110. a U-shaped notch; 112. air holes are formed; 2. a liquid storage hopper; 20. a bell mouth; 21. a bucket bottom; 3. a flow guide pipe; 30. a flow guide part; 31. absorbent paper; 4. a filter screen; 5. a pulling member; 50. a support plate; 51. vertical pulling plate, 6, glass slide.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Example 1: a slide making clamp for a cell slide maker, referring to fig. 1 and 2, comprises a box body 1 for clamping a slide glass, wherein the box body 1 comprises a front side plate 10, a rear side plate 11 and a bottom plate for connecting the front side plate 10 and the rear side plate 11. The upper edge of the rear side plate 11 is provided with a downward-concave U-shaped notch 110, and the upper edge of the front side plate 10 is lower than or equal to the height of the bottom of the U-shaped notch 110. This facilitates the operator to pinch the slide out of the cassette 1.

Referring to fig. 3, a transparent dyeing hole 100 is formed on the front side plate 10 at a position corresponding to a slide where a sample solution is to be applied. The liquid storage hopper 2 used for adding sample liquid is arranged at the position opposite to the box body 1, the liquid storage hopper 2 is communicated with the dyeing hole 100 through the guide pipe 3, and the front side plate 10 and the guide pipe 3 are integrally arranged.

The guide pipe 3 is internally provided with a flow guide part 30 which is obliquely arranged, and the flow guide part 30 gradually decreases from the dyeing hole 100 to the liquid storage hopper 2. When carrying out centrifugal motion like this, the sample liquid passes through honeycomb duct 3 directive slide glass from the opening part of liquid storage bucket 2, and under the effect of collision force, the sample liquid scatters on the surface of slide glass, forms thin adhesive layer, simultaneously because the effect of water conservancy diversion portion 30, remaining sample liquid can begin the refluence, flows into liquid storage bucket 2 again, can not cause around scribbling dyeing hole 100 and pile up, and then can not spread along the slide glass, has guaranteed that the background of slide glass is clear. The straight pipe with the constant diameter and arranged from the dyeing hole 100 to the liquid storage hopper 2 in an inclined downward mode is used as the guide pipe 3, and materials are convenient to obtain.

Referring to fig. 3, sometimes the centrifugation time is too long due to unfamiliarity or negligence of the worker, and a small amount of sample liquid still spreads out from the staining wells 100 under frequent washing action, which affects the clarity of the slide.

In order to solve the problem, the water absorbing paper 31 is added in the box body 1, the water absorbing paper 31 is positioned between the glass slide and the dyeing hole 100, and a through hole communicated with the dyeing hole 100 is formed in the position, corresponding to the dyeing hole 100, of the water absorbing paper 31, so that the sample liquid diffused from the dyeing hole 100 can be absorbed by the water absorbing paper 31, and the glass slide cannot be polluted.

One or more air holes 112 are formed in the rear side plate 11, so that when the slide holder is used for centrifugal slide making, gas between the glass slide 6 and the box body 1 can be rapidly discharged, the glass slide 6 is effectively attached to the rear side plate 11 of the box body 1, and then sample liquid can be effectively attached to an effective dyeing area of the glass slide 6.

The liquid storage bucket 2 is composed of a hemispherical bucket bottom 21 and a bell mouth 20 connected above the bucket bottom 21. The bell mouth 20 and the hopper bottom 21 are integrally manufactured, and the bell mouth 20 can enable a worker to conveniently drop or pour sample liquid into the liquid storage hopper 2; meanwhile, the hemispherical bucket bottom 21 can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom 21 is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide 6 after being dyed is further ensured.

Meanwhile, the sample solution contains impurities such as mucus, red blood cells and the like, and the impurities can influence the definition of the stained part of the glass slide 6, so the filter screen 4 is arranged in the liquid storage bucket 2. Filter screen 4 is the pocket form, and during the use the last border of filter screen 4 is taken and is put on the opening of stock solution fill 2, and the below stretches into in the stock solution fill 2, and the mesh diameter of filter screen 4 is about 6 microns, can filter mucus and red blood cell, and then makes the video picture after 6 scribbles of slide glass more clear.

Referring to fig. 3, in actual use, the absorbent paper 31 absorbs excess cells and liquid beyond the inner ring area, and because the opening is present and a large amount of liquid is absorbed, the absorbent paper 31 becomes soft and breakable, and when a subsequent worker takes out the slide 6 and the absorbent paper 31 together, the absorbent paper 31 stays in the case 1, which causes trouble to the subsequent work.

To solve this problem, a lifting member 5 for lifting the slide glass 6 and the water absorbing paper 31 is provided in the cassette body 1. The lifting piece 5 comprises a vertical pulling plate 51 which is vertically arranged and a supporting plate 50 which is fixedly connected to the lower end of the vertical pulling plate 51. When the lifting device is used, the lifting piece 5 is vertically inserted into the box body 1, one side of the vertical pulling plate 51 is attached to the rear side plate 11, and the bottom of the supporting plate 50 is attached to the bottom plate of the box body 1. The upper surface of the supporting plate 50 is in a horizontal state, and one side of the supporting plate, which is far away from the vertical pulling plate 51, is attached to the front side plate 10. Next, the slide glass 6 and the absorbent paper 31 are inserted, and the lower edges of the slide glass 6 and the absorbent paper 31 are supported on the pallet 50. After the slide 6 is taken out after the slide preparation, the lifting piece 51, the slide 6 and the absorbent paper 31 are simultaneously held by hands and then lifted upwards, and the supporting plate 50 can hold the absorbent paper 31 and lift upwards to separate from the box body 1, so that the absorbent paper 31 can not be retained in the slide preparing clamp.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims

1. The utility model provides a cell slide maker is with system piece holder, is including box body (1) that is used for the clamping slide glass, liquid storage bucket (2) and honeycomb duct (3) that are used for storing the appearance liquid, and box body (1) includes preceding curb plate (10), posterior lateral plate (11) and is used for connecting the bottom plate of preceding curb plate (10) and posterior lateral plate (11), the position department that corresponds slide glass (6) on preceding curb plate (10) has seted up and has painted hole (100), honeycomb duct (3) one end intercommunication is painted hole (100) and other end intercommunication liquid storage bucket (2), its characterized in that: the piece making clamp further comprises a lifting piece (5) in sliding fit with the box body (1), the lifting piece (5) comprises a vertical pulling plate (51) and a supporting plate (50) fixedly connected to the lower end of the vertical pulling plate (51), one side of the vertical pulling plate (51) is attached to the rear side plate (11), and one side, away from the vertical pulling plate (51), of the supporting plate (50) is attached to the front side plate (10); the liquid storage hopper (2) is composed of a hemispherical hopper bottom (21) and a bell mouth (20) connected above the hopper bottom (21), and the bottom of the hopper bottom (21) is lower than the lower end of the flow guide pipe (3).

2. The cell pelleter slide of claim 1, wherein: one or more through air holes (112) are formed in the rear side plate (11).

3. The cell pelleter slide of claim 2, wherein: be provided with filter screen (4) that can dismantle the connection in liquid storage bucket (2), filter screen (4) are the pocket form, the last border of filter screen (4) is taken and is put on the last border of liquid storage bucket (2) and the below of filter screen (4) stretches into in liquid storage bucket (2).

4. The cell pelleter slide of claim 3, wherein: the upper edge of the rear side plate (11) is provided with a downward-sunken U-shaped notch (110), and the upper edge of the front side plate (10) is lower than or equal to the height of the bottom of the U-shaped notch (110).