Negative pressure automatic smear, overflow dyeing machine
Technical field
The utility model relates to and a kind of cell, bacterium is carried out to the medical test of smear staining and the instrument and equipment of biological study, is specially negative pressure automatic smear, overflow dyeing machine.
Background technology
Cell, bacterium are carried out to the important step that smear staining film-making is medical test and biological study, there are at present two kinds of methods to cell, nacterial smear stained preparation: the one, traditional manual operation smear staining film-making, another kind is to utilize instrument and equipment automatic smear stained preparation.The required instrument and equipment of manual operation smear staining film-making is few, it is little to invest, but has following defect: 1, smear thickness relies on operating personnel's working experience, is difficult to control.2, the experience with operating personnel drips dyeing liquor, and randomness is larger, can not be quantitative, and the poor repeatability of Color.3, dyeing time, temperature can only guestimate, can not guarantee Color.The instrument and equipment with cytology automatic smear, dyeing function only has the U.S. to produce at present, its type has Auto-Cyte PREP instrument etc., its principle of work is: sample is carried out to gradient separations, centrifugal packed cells sample, sample is transferred in the special device that slide is installed, by cell natural subsidence, to slide, utilize the coating of sticking on slide that cell is fixed, then automatic staining completes film-making process.Utilize this equipment production effect good, but function is few, can only completes a kind of colouring method of pap staining; Complicated operation, apparatus expensive, the domestic quotation of a set of the said equipment is more than 1,000,000 yuan, consumables price, cost of use is high.
Summary of the invention
The purpose of this utility model is to provide a kind of negative pressure automatic smear, overflow dyeing machine automatically completing bacterium, cell smear, dyeing; Another object is to complete different colouring methods; Another object is the reproducible of Color; The 4th object is that recall rate is high, efficiency is high; The 5th object is the pollution of avoiding between sample; The 6th object is that handling safety is simple, manufacturing cost and cost of use low.
For achieving the above object, the utility model the utility model relates to a kind of negative pressure automatic smear, overflow dyeing machine, hydro-extractor is installed on body, hydro-extractor includes motor, axle and centrifugal turntable, below centrifugal turntable with on the body at centrifugal turntable mated position place, be provided with waste collecting device, on centrifugal turntable, be provided with glass stand, in the bottom of glass stand, be provided with glass stand hole, on the body above glass stand, be provided with the liquid feeding head supporting with glass stand position, this liquid feeding head is connected with one end of reagent pipeline, the other end of reagent pipeline is inserted in reagent container, on reagent pipeline, fixed displacement pump is installed, on body, be also provided with by controlling motor and realize controlling centrifugal turntable centrifugal rotation and location and controlling fixed displacement pump and start or quit work and the computing machine of amount of reagent.
Described negative pressure automatic smear, overflow dyeing machine are provided with a supporting liquid feeding head above each glass stand, and each liquid feeding head is connected with one end of a reagent pipeline, on each root reagent pipeline, a fixed displacement pump are installed.
Described negative pressure automatic smear, overflow dyeing machine, on centrifugal turntable, be provided with 24 equally distributed glass stands, above glass stand, be provided with 24 supporting liquid feeding heads, 24 liquid feeding heads are connected with one end of 24 reagent pipelines respectively, the other end of every three reagent pipelines is inserted in a reagent container, on each root reagent pipeline, a fixed displacement pump is installed.
Described negative pressure automatic smear, overflow dyeing machine are provided with the locating device for glass stand location on the body with glass stand mated position.
Described negative pressure automatic smear, overflow dyeing machine, described locating device is electromagnetic locator, is provided with an electromagnetic locator on the body below each glass stand.
Described negative pressure automatic smear, overflow dyeing machine are connected with sewer pipe and waste liquid box on waste collecting device, and this waste liquid box is the version of sealing, and waste liquid box is connected with negative pressure pump by negative tube.
Described negative pressure automatic smear, overflow dyeing machine, in glass stand, be placed with perforated filter film Double-layered cup, described perforated filter film Double-layered cup includes cup, in the bottom of cup, be provided with the cup hole supporting with glass stand hole, filtering membrane collet is installed in the inner chamber of cup, at the upper side of filtering membrane collet, filtering membrane is installed.
Described negative pressure automatic smear, overflow dyeing machine, inner chamber at cup is provided with interlayer inner sleeve, step in the bottom of interlayer inner sleeve is provided with interlayer inner sleeve, with interlayer inner sleeve in the interlayer inner sleeve bottom of step mated position be provided with interlayer inner sleeve buckle mouth, on filtering membrane collet, be provided with the lug supporting with interlayer inner sleeve buckle mouth.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, hydro-extractor is installed on body, hydro-extractor includes motor, axle and centrifugal turntable, below centrifugal turntable with on the body at centrifugal turntable mated position place, be provided with waste collecting device, on centrifugal turntable, be provided with glass stand, in the bottom of glass stand, be provided with glass stand hole, above glass stand, be provided with liquid feeding head, this liquid feeding head is connected with one end of reagent pipeline, the other end of reagent pipeline is inserted in reagent container, on reagent pipeline, fixed displacement pump is installed, on body, be also provided with and control the centrifugal rotation of centrifugal turntable and location and fixed displacement pump and start or quit work and the computing machine of amount of reagent, in glass stand, place perforated filter film Double-layered cup, described perforated filter film Double-layered cup includes cup, in the bottom of cup, is provided with cup hole, at the inner chamber of cup, interlayer inner sleeve is installed, bottom in interlayer inner sleeve is provided with filtering membrane, and filtering membrane collet is installed below filtering membrane.During use, to check that sample joins in the inner chamber of interlayer inner sleeve, under the centrifugation of the utility model negative pressure automatic smear, overflow dyeing machine hydro-extractor, check that the liquid in sample sees through the glass stand hole of the cup hole of filtering membrane, bottom of cup, glass stand bottom, entering into waste collecting device collects, bacterium or cell all evenly stick on the upper surface of filtering membrane, have realized the object of the utility model negative pressure automatic smear, overflow dyeing machine automatic smear.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, utilize the centrifugal rotation of computer control centrifugal turntable and location and fixed displacement pump start or quit work and amount of reagent, only the quantity of reagent container need be set according to the requirement of dyeing, in a reagent container, fill a kind of dyeing liquor, setting computer program, according to adding a kind of dyeing liquor → dyeing → centrifuging → waste liquid, enter into the step that another kind of dyeing liquor was located → added to waste collecting device collection → centrifugal turntable, by computer control, the utility model negative pressure automatic smear, overflow dyeing machine will accurately join different dyeing liquors and the amount needing in perforated filter film Double-layered cup according to the program of dyeing and requirement automatically successively, accurately control dyeing time, automatically complete whole dyeing course.Therefore, the utility model negative pressure automatic smear, overflow dyeing machine have been realized the object of automatic staining.
Due to the utility model negative pressure automatic smear, overflow dyeing machine utilizes the centrifugal rotation of computer control centrifugal turntable and location and fixed displacement pump start or quit work and amount of reagent, only need reagent container be set according to the requirement of coloured differently method, in a reagent container, fill a kind of dyeing liquor, the program of setting computer, just can and require according to the program of selected colouring method different dyeing liquors and the amount needing accurately to join successively in perforated filter film Double-layered cup, accurately control dyeing time, according to the colouring method of setting, automatically complete whole dyeing course, therefore, the utility model negative pressure automatic smear, overflow dyeing machine has been realized the object that automatically completes coloured differently method.
Because the utility model negative pressure automatic smear, overflow dyeing machine utilize the centrifugal rotation of computer control centrifugal turntable and location and fixed displacement pump, start or quit work and amount of reagent, the program of setting computer, according to the program of dyeing and requirement, different dyeing liquors and the amount needing are accurately joined in perforated filter film Double-layered cup successively, accurately control dyeing time, automatically complete whole dyeing course, therefore, can guarantee the repeatability of Color.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, it checks that the liquid in sample enters into waste collecting device collection through filtering membrane, check that bacterium or cell in sample all evenly stick on the upper surface of filtering membrane, the quantity of bacterium or cell is large, and its recall rate is high.
Because the utility model negative pressure automatic smear, overflow dyeing machine are provided with 24 glass stands, in each glass stand, place a perforated filter film Double-layered cup, each perforated filter film Double-layered cup completes smear, the dyeing of a sample, the utility model negative pressure automatic smear, overflow dyeing machine once can complete smear, the dyeing of 24 samples, so efficiency is high.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, its each sample is to utilize a perforated filter film Double-layered cup automatically to complete smear, dyeing, has therefore avoided the pollution between sample.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, on waste collecting device, be connected with sewer pipe and waste liquid box, this waste liquid box is the version of sealing, waste liquid box is connected with negative pressure pump by negative tube, after negative pressure pump is opened, by negative tube, waste liquid box, sewer pipe, in waste collecting device, produce negative pressure, so just can be better by the waste collection of centrifugal generation, and can effectively collect the waste liquid mist of centrifugal generation, avoid waste liquid mist to fly away outside waste collecting device.
Simultaneously, due to the utility model negative pressure automatic smear, overflow dyeing machine, whole smear, dyeing course only need manually in perforated filter film Double-layered cup, to add sample, from glass stand, to take out perforated filter film Double-layered cup, open bowl cover, take out interlayer inner sleeve and put into dryer and dry, from putting in interlayer, take off filtering membrane again, its smear, dyeing course all utilize computer control automatically to complete, therefore safe and convenient to use.
Due to the utility model negative pressure automatic smear, overflow dyeing machine, mainly by hydro-extractor, computing machine and fixed displacement pump etc., formed, to compare with Auto-Cyte PREP instrument etc., its manufacturing cost and use cost are all low.
In sum, the utility model negative pressure automatic smear, overflow dyeing machine realized automatically complete to bacterium, cell smear, dyeing, can complete different colouring methods, Color reproducible, recall rate is high, efficiency is high, can avoid pollution between sample, handling safety is simple, manufacturing cost and the low object of cost of use.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model negative pressure automatic smear, overflow dyeing machine are described further.
Figure 1 shows that the front view cut-open view schematic diagram of the hydro-extractor part of the utility model negative pressure automatic smear, overflow dyeing machine.
The reagent container 13, reagent pipeline 16, the liquid feeding head 7 that Figure 2 shows that the utility model negative pressure automatic smear, overflow dyeing machine connect and distribution schematic diagram.
Fig. 3 is the front view cut-open view schematic diagram of the Double-layered cup of perforated filter film shown in Fig. 18.
Fig. 4 is the partial sectional view schematic diagram of the 8e of interlayer inner sleeve shown in Fig. 3.
Fig. 5 is the 8c of filtering membrane collet shown in Fig. 3 upward view schematic diagram.
1, body, 2, waste collecting device, 3, axle, 4, sewer pipe, 5, electromagnetic locator, 6, glass stand, 6a, glass stand hole, 7, liquid feeding head, 8, perforated filter film Double-layered cup, 8a, cup, 8b, cup hole, 8c, filtering membrane collet, 8d, filtering membrane, 8e, interlayer inner sleeve, 8f, bowl cover, 8g, bowl cover envelope, 8h, interlayer inner sleeve buckle mouth, 8k, step in interlayer inner sleeve, 8m, lug, 8n, protruding of cross, 9, centrifugal turntable, 10, waste liquid box, 11, negative tube, 12, negative pressure pump, 13, reagent container, 14, reagent extra heavy pipe, 15, fixed displacement pump, 16, reagent pipeline.
Embodiment
Figure 1 shows that the hydro-extractor part of the utility model negative pressure automatic smear, overflow dyeing machine.In frame 1, motor is installed, axle 3 is connected with motor, centrifugal turntable 9 is installed on axle 3, on centrifugal turntable 9, be evenly provided with 24 glass stands 6, also can on centrifugal turntable 9, be symmetrical arranged 24 glass stands 6, its objective is the balance that guarantees centrifugal turntable 9, an electromagnetic locator 5 is installed in the frame 1 below each glass stand 6, this electromagnetic locator 5 can be sucker electromagnet, in the bottom of glass stand 6, be provided with glass stand hole 6a, below centrifugal turntable 9 with on the body 1 at centrifugal turntable 9 mated position places, be provided with waste collecting device 2, this waste collecting device 2 is designed to the form of recessed " pot ", at its lowest part, be connected with sewer pipe 4 and waste liquid box 10, the version that this waste liquid box 10 is sealing, waste liquid box 10 is connected with negative pressure pump 12 by negative tube 11, after negative pressure pump 12 is opened, by negative tube 11, waste liquid box 10, sewer pipe 4, in waste collecting device 2, produce negative pressure, on the body 1 at mated position place above glass stand 6, liquid feeding head 7 is installed, perforated filter film Double-layered cup 8 is placed in glass stand 6.The effect of electromagnetic locator 5 is when centrifugal turntable 9 centrifugal rotation, effect due to centrifugal force, glass stand 6 outwards overturns, when centrifugal turntable 9 centrifugal rotations finish, effect due to coherence, glass stand 6 can swing back and forth around the axle between itself and centrifugal turntable 9, for rapidly by the vertical static location of glass stand 6, be convenient to the dyeing liquor of annotating, utilize the electromagnetic attraction effect of electromagnetic locator 5, by the rapid vertical static location of glass stand 6, like this liquid feeding head 7 could be effectively to the dyeing liquor of annotating in perforated filter film Double-layered cup 8.
The reagent container 13, reagent pipeline 16, the liquid feeding head 7 that Figure 2 shows that the utility model automatic smear, overflow dyeing machine connect and distribution schematic diagram.On body 1, be provided with reagent vessel holder, in this reagent vessel holder, be placed with 8 reagent containers 13, in each reagent container 13, be inserted with a reagent extra heavy pipe 14, a reagent extra heavy pipe 14 is connected with three reagent pipelines 16, have 24 reagent pipelines 16 altogether, each root reagent pipeline 16 is connected with a liquid feeding head 7, have 24 liquid feeding heads 7 altogether, on each root reagent pipeline 16, a fixed displacement pump 15 is installed, have 24 fixed displacement pumps 15 altogether, fixed displacement pump 15 can be selected peristaltic pump, and liquid feeding head 7 is fixed on body 1, supporting with 24 glass stand 6 positions on centrifugal turntable 9.
Be illustrated in figure 3 the perforated filter film Double-layered cup 8 of the utility model automatic smear, overflow dyeing machine, Fig. 4 is the 8e of interlayer inner sleeve shown in Fig. 3, and Fig. 5 is the 8c of filtering membrane collet shown in Fig. 3.Include cup 8a, in the bottom of cup 8a, be provided with cup hole 8b, inner chamber at cup 8a is provided with interlayer inner sleeve 8e, bottom at interlayer inner sleeve 8e inner chamber is provided with step 8k in interlayer inner sleeve, with this interlayer inner sleeve in have interlayer inner sleeve buckle mouth 8h on the interlayer inner sleeve 8e at step 8k mated position place, on filtering membrane collet 8c, be provided with the lug 8m supporting with interlayer inner sleeve buckle mouth 8h, utilize this lug 8m to be stuck in interlayer inner sleeve buckle mouth 8h, filtering membrane collet 8c is arranged on interlayer inner sleeve 8e, filtering membrane 8d is placed on filtering membrane collet 8c upper side, be pressed in step 8k in interlayer inner sleeve above fixes simultaneously, downside at filtering membrane collet 8c is also provided with protruding 8n of cross, when assembling filtering membrane collet 8c and interlayer inner sleeve 8e, utilize protruding 8n application of force of this cross that the lug 8m of filtering membrane collet 8c is stuck in interlayer inner sleeve buckle mouth 8h, in the upper end of cup 8a, be provided with bowl cover 8f, on bowl cover 8f, be provided with bowl cover hole, on bowl cover 8f, with adhesive sticker, be pasted with bowl cover envelope 8g.While not using, utilize bowl cover envelope 8g that bowl cover hole is sealed; During use, bowl cover is sealed to 8g and from bowl cover 8f, takes off or directly bowl cover is sealed 8f and do the bowl cover hole of bringing to light out, by bowl cover hole to annotate in interlayer inner sleeve 8 e inspection specimen, dyeing liquor.
The utility model negative pressure automatic smear, overflow dyeing machine, during use, in glass stand 6, place perforated filter film Double-layered cup 8, in reagent container 13, fill corresponding dyeing liquor, the corresponding a kind of dyeing liquor of every three liquid feeding heads 7, can give three perforated filter film Double-layered cups 8 filling same dyeing liquors, liquid feeding head 7 does not move simultaneously.The process of the utility model negative pressure automatic smear, overflow dyeing machine smear staining is: to adding in the perforated filter film Double-layered cup 8 in glass stand 6 specimen fluids → the utilize liquid in computer control centrifugal turntable 9 centrifugal rotation → inspection samples to see through cup hole 8b, the glass stand hole 6a of glass stand 6 bottoms of filtering membrane 8d, cup 8a bottom, entering into waste collecting device 2 collects, then by sewer pipe 4, enter into waste liquid box 10 → bacterium or cell adherence on filtering membrane 8d, complete smear.Open negative pressure pump 12, in waste collecting device 2, produce negative pressure, so just better by the waste collection of centrifugal generation, and can effectively collect the waste liquid mist of centrifugal generation, avoid waste liquid mist to fly away to waste collecting device.After completing smear, start dyeing procedure: utilize computer control centrifugal turntable 9 location, now, the liquid feeding head 7 of corresponding dyeing liquor of annotating is aimed at → is utilized the corresponding fixed displacement pump 15 of computer control with corresponding perforated filter film Double-layered cup 8 and starts or quit work, control the amount of reagent → computer control centrifugal turntable 9 centrifugal rotations → waste liquid that dyes → utilize simultaneously and see through filtering membrane 8d, the cup hole 8b of cup 8a bottom, the glass stand hole 6a of glass stand 6 bottoms, entering into waste collecting device 2 collects, then by sewer pipe 4, enter into waste liquid box 10, complete a kind of dyeing liquor dyeing in dyeing procedure, computing machine is according to the program of setting, automatically enter successively lower a kind of dyeing liquor dyeing, according to program and the requirement of dyeing, successively to each dyeing liquor dyeing, until complete whole dyeing procedure.Take out afterwards perforated filter film Double-layered cup 8, take out interlayer inner sleeve 8e, utilize drying machine drying, then take out filtering membrane 8d from interlayer inner sleeve 8e.
The utility model negative pressure automatic smear, overflow dyeing machine, can also on the body 1 above centrifugal turntable 9, drying unit be installed, after completing smear, dyeing procedure, utilize computer control drying unit that the filtering membrane 8d in perforated filter film Double-layered cup 8 is dried, complete film-making process, take out afterwards filtering membrane 8d.