CN106248978A - A kind of microporous filter membrane cup fixes, heat and negative pressure control method and fixed support thereof - Google Patents

A kind of microporous filter membrane cup fixes, heat and negative pressure control method and fixed support thereof Download PDF

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Publication number
CN106248978A
CN106248978A CN201610642966.4A CN201610642966A CN106248978A CN 106248978 A CN106248978 A CN 106248978A CN 201610642966 A CN201610642966 A CN 201610642966A CN 106248978 A CN106248978 A CN 106248978A
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China
Prior art keywords
filter membrane
glass stand
microporous filter
base plate
negative
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CN201610642966.4A
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Chinese (zh)
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CN106248978B (en
Inventor
彭钧
陈昌杰
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HUNAN TIANQI MEDICAL NEW TECHNOLOGY Co Ltd
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HUNAN TIANQI MEDICAL NEW TECHNOLOGY Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The invention discloses that a kind of microporous filter membrane cup is fixed, heated and negative pressure control method and fixed support thereof, fixed support it include that flexible glass stand, flexible glass stand are closely bonded formed by microporous filter membrane cup and glass stand base plate;On glass stand base plate, the corresponding position installing microporous filter membrane cup is provided with glass stand base plate mesh, the underface of glass stand base plate mesh is provided with negative-pressure sealing bowl, in negative-pressure sealing bowl is embedded in supporting plate and being locked on glass stand base plate by clip, described negative-pressure sealing bowl lower end connects the negative pressure equipment making its content attract downwards.It is an object of the invention to provide a kind of microporous filter membrane cup of offer to fix, heat and negative pressure control method and fixed support thereof, automatization is facilitated quickly to fix microporous filter membrane cup, realize fixing, dyeing, vacuum cavitations, the automation integrated operation of heating, decrease operation sequence, saving the operating time, it is higher that sample program of transfer when detection reduces precision.

Description

A kind of microporous filter membrane cup fixes, heat and negative pressure control method and fixed support thereof
Technical field
The present invention relates to one uses membrane separation technique to test, detect in the fields such as medical science, biology, environmentology Automatization fix, heat and negative pressure control method.
Background technology
Tangible material in using microporous filter membrane to make a collection of specimens and directly filter membrane is carried out transparent processing and put under microscope Carrying out observing is a novel morphologic detection technology, can be widely applied to the small tangible of medical test and other field Material detects, but owing to microporous filter membrane is soft and rapid wear causes having one side to be limited to, the method for use is substantially It is affixed directly to be mechanically fixed at the bottom of Double-layered cup or by Double-layered cup.
Patent one: Chinese Patent Application No. 201010529398.X, a kind of cell or the dyeing of antibacterial, flaking method, its Disclose a kind of cell in medical test and biological study or the dyeing of antibacterial, flaking method, using filter membrane as specimen Sheet substrate carries out dyeing, film-making, uses filtration type Double-layered cup as dyeing, film-making vessel, described filtration type Double-layered cup, cup Perisporium close, be only provided with hole on top and bottom, cup be provided with and can will be divided into upper and lower two-part filtration inside cup Film, under normal pressure, the liquid such as staining reagent is not passed through by the filter membrane being provided with in container, by the pressure arranged on filter membrane both sides Difference, retains antibacterial or cell, and is contaminated through the mode got rid of through filter membrane by the liquid beyond cell or antibacterial Color film-making, equipment can be utilized to be automatically performed cell for the present invention and nacterial smear, dyeing, film-making and use are simple, cost of use is low.
Its flaking method announced and photo making cup, when using automation equipment operation, difficulty is bigger.
Patent two: Chinese patent ZL201320852375.1, discloses a kind of negative pressure automatic smear, staining machine, at body On be provided with the centrifuge of centrifugal turntable, the body below centrifugal turntable is provided with waste collecting device, at centrifugal turntable It is provided with 24 glass stands, glass stand is placed with perforated filter film Double-layered cup, the body above glass stand is provided with 24 liquid feedings Head, this liquid feeding head is connected with reagent container by reagent pipeline, is provided with dosing pump, also installs on body on reagent pipeline Have and control centrifugal turntable centrifugal rotation and location and control dosing pump start or quit work and the computer of amount of reagent, this reality With New Energy be automatically performed to antibacterial, cell smear, dyeing, can complete different colouring method, Color reproducible, Recall rate is high, efficiency is high, operate safe and simple, manufacturing cost and cost of use is low.
It does not relate to heating operation (the sample needs after filtering when utilizing microporous filter membrane to carry out Senile Mouse Detect after drying), so need extra equipment and operation sequence, cause detecting relative complex loaded down with trivial details;It is corresponding simultaneously Test cup fixed mount is directed to the cup body structure that existing patent is a kind of, in its actual mechanical process, and picking and placeing of micropore filtering film Comparatively laborious, and it is higher to operate requirement, unsuitable for full-automatic operation, reduces efficiency and the precision of detection.
Summary of the invention
It is an object of the invention to for problem above, it is provided that a kind of microporous filter membrane cup is fixed, heated and negative pressure control method And fixed support, facilitate automatization quickly to fix microporous filter membrane cup, it is achieved fixing, dyeing, vacuum cavitations, the automatization of heating One operation, decreases operation sequence, saves the operating time, and it is higher that sample program of transfer when detection reduces precision.
For realizing object above, the technical solution used in the present invention is:
A kind of microporous filter membrane cup fixed support, it includes flexible glass stand (3), and flexible glass stand (3) fixes microporous filter membrane cup by being used for (4) the glass stand base plate (2) that flexible material and its bottom part down are arranged closely bonds composition;In glass stand base plate (2) upper correspondence peace The position of dress microporous filter membrane cup (4) is provided with glass stand base plate mesh (7), is provided with negative in the underface of glass stand base plate mesh (7) Means of press seals bowl (8), is locked at glass stand base plate (2) in described negative-pressure sealing bowl (8) is embedded in supporting plate (1) and by clip (10) On, described negative-pressure sealing bowl (8) lower end connects the negative pressure equipment making its content attract downwards.
Further, in described supporting plate (1), heater (9) is installed.
Further, the top of described negative-pressure sealing bowl (8) is provided with obturating cup seal (6), its higher than supporting plate (1) and The surrounding being centered around glass stand base plate mesh (7) forms sealing structure.
Further, described flexible glass stand (3) is provided with several clear openings, and with microporous filter membrane cup (4) to be fixed One_to_one corresponding.
The present invention discloses and use above-mentioned support bracket fastened control method:
A kind of microporous filter membrane cup is fixed, is heated and negative pressure control method, and it includes following a few part:
1), by flexible glass stand (3), supporting plate (1), negative-pressure sealing bowl (8), clip (10) with the use of composition, microporous filter membrane It is internal that cup (4) loads flexible carrier (3);And glass stand base plate mesh (7) during microporous filter membrane (5) is close to glass stand base plate (2) bottom it;
2), by microporous filter membrane cup (4) bottom periphery with flexible glass stand (3) being formed and seal, glass stand base plate is close to by negative-pressure sealing bowl (8) (2) without mesh portion and be centered around glass stand base plate mesh (7) surrounding formed seal structure;
3), negative-pressure sealing bowl (8) lower end connecting the negative pressure equipment making its content attract downwards, negative pressure passes through glass stand base plate net Hole (7) is that liquid passes through microporous filter membrane (5) filtration offer power;
4), by installing heater (9) in supporting plate (1), thermal source is provided for microporous filter membrane (5);
5), flexible glass stand (3) is combined closely with supporting plate (1) by clip (10).
Further, described flexible glass stand (3) is by having the flexible material of some clear openings and opening at the bottom of meshed glass stand Plate (2) closely bonds composition, lays the mesh portion on hole alignment glass stand base plate (2) of microporous filter membrane cup (4), glass stand base plate (2) quickly the even heat that supporting plate (1) produces can be passed to microporous filter membrane (5), make microporous filter membrane cup (4) setting Operate under fixed temperature.
Further, supporting plate (1) is provided with negative-pressure sealing bowl (8), heater (9), clip (10), negative-pressure sealing The obturating cup seal (6) of bowl (8) is higher than supporting plate (1) plane 1-5mm, when buckling clip (10) with glass stand base plate (2) nothing Mesh portion forms the generation sealing effectiveness that closely cooperates.
Further, flexible glass stand (3) can be by unclamping the separate unit that clip (10) separates with supporting plate (1), For loading microporous filter membrane cup (4) in batches, position, multiple hole can be arranged above each flexible glass stand (3), load multiple micropore Filter membrane cup (4);Negative-pressure sealing bowl (8) on supporting plate (1) and position, glass stand hole one_to_one corresponding.
Further, heater (9) provides temperature guarantee according to requirement of experiment, and heater (9) includes heater element And temperature control component.
Further, negative-pressure sealing bowl (8) is obtained stable negative pressure by external vacuum pump and negative pressure control apparatus.
Beneficial effects of the present invention: the invention provides a kind of microporous filter membrane cup fix, heat and negative pressure control method and Its fixed support, facilitates automatization quickly to fix microporous filter membrane cup, it is achieved fixing, dyeing, vacuum cavitations, the automatization one of heating Gymnastics is made, and decreases operation sequence, saves the operating time, and it is higher that sample program of transfer when detection reduces precision.
1, fast and easy carries out picking and placeing microporous filter membrane cup.
2, during observing, it is not necessary to again separate microporous filter membrane, it is to avoid microporous filter membrane crushes, and facilitates microscopy to operate, letter Change operation sequence, save the operating time.
Accompanying drawing explanation
Fig. 1 is that the present invention fixes heated holder structural representation.
Fig. 2 is microporous filter membrane cup and microporous filter membrane structural representation in Fig. 1.
Described in figure, label character is expressed as: 1, supporting plate;2, glass stand base plate;3, flexible glass stand;4, microporous filter membrane cup;5, micro- Hole filter membrane;6, obturating cup seal;7, glass stand base plate mesh;8, negative-pressure sealing bowl;9, heater;10, clip;41, cup Body annular groove;42, ring plate mouth is taken;5, microporous filter membrane;51, dress film ring.
Detailed description of the invention
In order to make those skilled in the art be more fully understood that technical scheme, below in conjunction with the accompanying drawings the present invention is entered Row describes in detail, and the description of this part is only exemplary and explanatory, should not have any restriction to protection scope of the present invention Effect.
As it is shown in figure 1, one technical scheme of the present invention: a kind of microporous filter membrane cup fixed support, it includes flexible glass stand 3, The glass stand base plate 2 that flexible glass stand 3 is arranged by the flexible material and its bottom part down for fixing microporous filter membrane cup 4 closely bonds group Become;On glass stand base plate 2, the corresponding position installing microporous filter membrane cup 4 is provided with glass stand base plate mesh 7, at glass stand base plate mesh 7 Underface be provided with negative-pressure sealing bowl 8, be locked at cup in described negative-pressure sealing bowl 8 is embedded in supporting plate 1 and by clip 10 On frame base plate 2, described negative-pressure sealing bowl 8 lower end connects the negative pressure equipment making its content attract downwards.
Preferably, in described supporting plate 1, heater 9 is installed.
Preferably, the top of described negative-pressure sealing bowl 8 is provided with obturating cup seal 6, and it is higher than supporting plate 1 and is centered around cup The surrounding of frame base plate mesh 7 is formed and seals structure.
Preferably, described flexible glass stand 3 is provided with several clear openings, and with microporous filter membrane cup to be fixed 4 one a pair Should.
Disclosing the microporous filter membrane cup 4 being used in combination with the present invention in Fig. 2, its bottom of cup end face is provided with cup card Annular groove 41, matched microporous filter membrane 5 upper surface is fixed with dress film ring 51, and it is embedded in cup annular groove 41, at cup Body bottom outside be provided with take off dress film ring 51 take ring plate mouth 42.
The present invention discloses and use above-mentioned support bracket fastened control method:
A kind of microporous filter membrane cup is fixed, is heated and negative pressure control method, and it includes following a few part:
1), load soft by flexible glass stand 3, supporting plate 1, negative-pressure sealing bowl 8, clip 10 with the use of composition, microporous filter membrane cup 4 Property frame 3 internal;And the glass stand base plate mesh 7 during microporous filter membrane 5 is close to glass stand base plate 2 bottom it;
2), with flexible glass stand 3, microporous filter membrane cup 4 bottom periphery being formed sealing, negative-pressure sealing bowl 8 is close to glass stand base plate 2 without net Eye portion and be centered around glass stand base plate mesh 7 surrounding formed seal structure;
3), negative-pressure sealing bowl 8 lower end connecting the negative pressure equipment making its content attract downwards, negative pressure passes through glass stand base plate mesh 7 filter offer power for liquid by microporous filter membrane 5;
4), by supporting plate 1, heater 9 is installed, provides thermal source for microporous filter membrane 5;
5), flexible glass stand 3 is combined closely with supporting plate 1 by clip 10.
Preferably, described flexible glass stand 3 is by having the flexible material of some clear openings and to open meshed glass stand base plate 2 tight Close bonding composition, lay microporous filter membrane cup 4 hole alignment glass stand base plate 2 on mesh portion, glass stand base plate 2 can quickly by The even heat that supporting plate 1 produces passes to microporous filter membrane 5, makes microporous filter membrane cup 4 to operate at a set temperature.
Preferably, supporting plate 1 is provided with negative-pressure sealing bowl 8, heater 9, clip 10, the sealing of negative-pressure sealing bowl 8 Bowl seal 6, higher than supporting plate 1 plane 1-5mm, closely cooperates without mesh portion formation with glass stand base plate 2 when buckling clip 10 Produce sealing effectiveness.
Preferably, flexible glass stand 3 can be by unclamping the separate unit that clip 10 separates with supporting plate 1, for batch Load microporous filter membrane cup 4, position, multiple hole can be arranged above each flexible glass stand 3, load multiple microporous filter membrane cup 4;Supporting plate 1 On negative-pressure sealing bowl 8 and position, glass stand hole one_to_one corresponding.
Preferably, heater 9 provides temperature guarantee according to requirement of experiment, and heater 9 includes heater element and temperature Control element.
Preferably, negative-pressure sealing bowl 8 is obtained stable negative pressure by external vacuum pump and negative pressure control apparatus.
It should be noted that in this article, term " includes ", " comprising " or its any other variant are intended to non-row Comprising of his property, so that include that the process of a series of key element, method, article or equipment not only include those key elements, and And also include other key elements being not expressly set out, or also include intrinsic for this process, method, article or equipment Key element.
Principle and the embodiment of the present invention are set forth by specific case used herein, the explanation of above example It is only intended to help to understand method and the core concept thereof of the present invention.The above is only the preferred embodiment of the present invention, should When pointing out, due to the finiteness of literal expression, and objectively there is unlimited concrete structure, for the common skill of the art For art personnel, under the premise without departing from the principles of the invention, it is also possible to make some improvement, retouch or change, it is also possible to will Above-mentioned technical characteristic is combined by rights;These improve retouching, change or combine, or the most improved structure by invention Think and technical scheme directly applies to other occasion, be regarded as protection scope of the present invention.

Claims (10)

1. a microporous filter membrane cup fixed support, it is characterised in that it includes flexible glass stand (3), flexible glass stand (3) is by for solid The glass stand base plate (2) of the flexible material and the setting of its bottom part down of determining microporous filter membrane cup (4) closely bonds composition;At glass stand base plate (2) position that upper correspondence installs microporous filter membrane cup (4) is provided with glass stand base plate mesh (7), glass stand base plate mesh (7) just under Side is provided with negative-pressure sealing bowl (8), is locked in described negative-pressure sealing bowl (8) is embedded in supporting plate (1) and by clip (10) On glass stand base plate (2), described negative-pressure sealing bowl (8) lower end connects the negative pressure equipment making its content attract downwards.
A kind of microporous filter membrane cup fixed support the most according to claim 1, it is characterised in that install in described supporting plate (1) There is heater (9).
A kind of microporous filter membrane cup fixed support the most according to claim 1, it is characterised in that described negative-pressure sealing bowl (8) Top be provided with obturating cup seal (6), it is higher than supporting plate (1) and is centered around the surrounding of glass stand base plate mesh (7) and is formed close Seal structure.
A kind of microporous filter membrane cup fixed support the most according to claim 1, it is characterised in that on described flexible glass stand (3) Be provided with several clear openings, and with microporous filter membrane cup (4) one_to_one corresponding to be fixed.
5. a microporous filter membrane cup is fixed, is heated and negative pressure control method, it is characterised in that it includes following a few part:
, by flexible glass stand (3), supporting plate (1), negative-pressure sealing bowl (8), clip (10) with the use of composition, microporous filter membrane cup (4) flexible carrier (3) is loaded internal;And glass stand base plate mesh (7) during microporous filter membrane (5) is close to glass stand base plate (2) bottom it;
, microporous filter membrane cup (4) bottom periphery formed with flexible glass stand (3) seal, glass stand base plate is close to by negative-pressure sealing bowl (8) (2) without mesh portion and be centered around glass stand base plate mesh (7) surrounding formed seal structure;
, negative-pressure sealing bowl (8) lower end connected make the negative pressure equipment that its content attracts downwards, negative pressure passes through glass stand base plate mesh (7) it is that liquid passes through microporous filter membrane (5) filtration offer power;
, by installing heater (9) in supporting plate (1), provide thermal source for microporous filter membrane (5);
, flexible glass stand (3) combined closely with supporting plate (1) by clip (10).
A kind of microporous filter membrane cup the most according to claim 5 is fixed, is heated and negative pressure control method, it is characterised in that institute State flexible glass stand (3) by having the flexible material of some clear openings and opening meshed glass stand base plate (2) and closely bond and form, peace Putting the mesh portion on hole alignment glass stand base plate (2) of microporous filter membrane cup (4), glass stand base plate (2) can be quickly by supporting plate (1) The even heat produced passes to microporous filter membrane (5), makes microporous filter membrane cup (4) to operate at a set temperature.
A kind of microporous filter membrane cup the most according to claim 5 is fixed, is heated and negative pressure control method, it is characterised in that torr Negative-pressure sealing bowl (8), heater (9), clip (10), the obturating cup seal of negative-pressure sealing bowl (8) are installed on plate (1) (6) higher than supporting plate (1) plane 1-5mm, closely cooperate without mesh portion formation with glass stand base plate (2) when buckling clip (10) Produce sealing effectiveness.
A kind of microporous filter membrane cup the most according to claim 5 is fixed, is heated and negative pressure control method, it is characterised in that soft Property glass stand (3) can be by unclamping the separate unit that clip (10) separates with supporting plate (1), for loading microporous filter membrane in batches Cup (4), can arrange position, multiple hole above each flexible glass stand (3), load multiple microporous filter membrane cup (4);On supporting plate (1) Negative-pressure sealing bowl (8) and position, glass stand hole one_to_one corresponding.
A kind of microporous filter membrane cup the most according to claim 5 is fixed, is heated and negative pressure control method, it is characterised in that add Thermal (9) provides temperature guarantee according to requirement of experiment, and heater (9) includes heater element and temperature control component.
A kind of microporous filter membrane cup the most according to claim 5 is fixed, is heated and negative pressure control method, it is characterised in that negative Means of press seals bowl (8) is obtained by external vacuum pump and negative pressure control apparatus stablizes negative pressure.
CN201610642966.4A 2016-08-08 2016-08-08 A kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket Active CN106248978B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108693008A (en) * 2018-07-25 2018-10-23 湖南省天骑医学新技术股份有限公司 A kind of miillpore filter cup collection device for miillpore filter transfer
CN115406188A (en) * 2022-08-16 2022-11-29 湖南省天骑医学新技术股份有限公司 Rapid drying treatment method and system for filter membrane

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CN102042924A (en) * 2010-10-30 2011-05-04 湖南省天骑医学新技术有限公司 Dyeing and flaking method for cells or bacteria
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CN103616267A (en) * 2013-11-13 2014-03-05 厦门宝太生物科技有限公司 Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear
CN203732340U (en) * 2013-12-23 2014-07-23 湖南省天骑医学新技术有限公司 Automatic negative pressure staining and dyeing machine
CN203894092U (en) * 2013-12-23 2014-10-22 湖南省天骑医学新技术有限公司 Filtering membrane interlayer cup
CN205982295U (en) * 2016-08-08 2017-02-22 湖南省天骑医学新技术股份有限公司 Millipore filtration cup fixed bolster

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US6225130B1 (en) * 1998-02-19 2001-05-01 Fuji Photo Film Co., Ltd. Method of separating serum from whole blood
CN102042924A (en) * 2010-10-30 2011-05-04 湖南省天骑医学新技术有限公司 Dyeing and flaking method for cells or bacteria
CN202141618U (en) * 2010-10-30 2012-02-08 湖南省天骑医学新技术有限公司 Cytological and bacterial staining and preparation machine
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CN108693008A (en) * 2018-07-25 2018-10-23 湖南省天骑医学新技术股份有限公司 A kind of miillpore filter cup collection device for miillpore filter transfer
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CN115406188A (en) * 2022-08-16 2022-11-29 湖南省天骑医学新技术股份有限公司 Rapid drying treatment method and system for filter membrane
CN115406188B (en) * 2022-08-16 2023-12-15 湖南省天骑医学新技术股份有限公司 Filter membrane rapid drying treatment method and system

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