CN106289906A - A kind of microporous filter membrane pelletizer and the assembly method when film-making and microscope are observed thereof - Google Patents
A kind of microporous filter membrane pelletizer and the assembly method when film-making and microscope are observed thereof Download PDFInfo
- Publication number
- CN106289906A CN106289906A CN201610645966.XA CN201610645966A CN106289906A CN 106289906 A CN106289906 A CN 106289906A CN 201610645966 A CN201610645966 A CN 201610645966A CN 106289906 A CN106289906 A CN 106289906A
- Authority
- CN
- China
- Prior art keywords
- filter membrane
- microporous filter
- cup
- film
- pelletizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 239000012528 membrane Substances 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000003756 stirring Methods 0.000 claims description 2
- 239000012780 transparent material Substances 0.000 claims description 2
- 238000000386 microscopy Methods 0.000 abstract description 4
- 238000007689 inspection Methods 0.000 abstract description 3
- 239000012982 microporous membrane Substances 0.000 abstract 1
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 13
- 238000004043 dyeing Methods 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000010339 medical test Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The invention discloses a kind of microporous filter membrane pelletizer and the assembly method when film-making and microscope are observed thereof, device includes cup and arranges the microporous filter membrane bottom it, it is provided with cup annular groove on described cup lower surface, and microporous membrane surface is fixed with the dress film ring corresponding with cup annular groove, the dress film ring on microporous filter membrane is freely embeddable in cup annular groove；And be provided with in the bottom of cup and to take ring plate mouth.It is an object of the invention to provide a kind of microporous filter membrane pelletizer and the assembly method when film-making and microscope are observed thereof, have employed a kind of brand-new structure and film-making, microscopy method, conveniently carry out the disengaging after microporous filter membrane film-making, and fast and easy carries out fixed mirror inspection on micro slide.
Description
Technical field
The present invention relates to one uses membrane separation technique to test, detect in the fields such as medical science, biology, environmentology
During carry out the apparatus and method of film-making, dyeing, be specially a kind of microporous filter membrane pelletizer, and it be in film-making and micro-
Assembly method during sem observation.
Background technology
Tangible material in using microporous filter membrane to make a collection of specimens and directly filter membrane is carried out transparent processing and put under microscope
Carrying out observing is a novel morphologic detection technology, can be widely applied to the small tangible of medical test and other field
Material detects, but owing to microporous filter membrane is soft and rapid wear causes having one side to be limited to, the method for use is substantially
It is affixed directly to be mechanically fixed at the bottom of Double-layered cup or by Double-layered cup.
Such as Chinese Patent Application No. 201010529398.X, a kind of cell or the dyeing of antibacterial, flaking method, it is open
A kind of cell in medical test and biological study or the dyeing of antibacterial, flaking method, using filter membrane as specimen chip base
Sheet carries out dyeing, film-making, uses filtration type Double-layered cup as dyeing, film-making vessel, described filtration type Double-layered cup, the week of cup
Wall is closed, and is only provided with hole on top and bottom, is provided with and can will be divided into upper and lower two-part filter membrane inside cup, often in cup
The liquid such as pressure staining reagent are not passed through by the filter membrane being provided with in container, by the pressure differential arranged on filter membrane both sides, right
Antibacterial or cell retain, and through the mode got rid of, the liquid beyond cell or antibacterial is carried out dyeing system through filter membrane
Sheet, equipment can be utilized to be automatically performed cell for the present invention and nacterial smear, dyeing, film-making and use are simple, cost of use is low.
Although above method can use well, however it is necessary that and carry out manually adding reagent operation and taking off micro-of complexity
The operating process of hole filter membrane, owing to microporous filter membrane is relatively light, also needs to when carrying out microscope and observing filter micropore on micro slide
Film fixes (cover and add coverslip) again.Therefore when using automation equipment operation, difficulty is bigger.
Summary of the invention
It is an object of the invention to for problem above, it is provided that a kind of microporous filter membrane pelletizer and at film-making and microscope
Assembly method during observation, have employed a kind of brand-new structure and film-making, microscopy method, after conveniently carrying out microporous filter membrane film-making
Depart from, and fast and easy carries out fixed mirror inspection on micro slide.
For realizing object above, the technical solution used in the present invention is: a kind of microporous filter membrane pelletizer, it includes cup
(1) and the microporous filter membrane (2) bottom it is set, described cup (1) lower surface is provided with cup annular groove (4), and micropore
Filter membrane (2) surface is fixed with dress film ring (3) corresponding with cup annular groove (4), and dress film ring (3) on microporous filter membrane (4) is certainly
By being embedded in cup annular groove (4)；And be provided with in the bottom of cup (1) and to take ring plate mouth (104).
Further, described cup (1) is revolving body, and corresponding dress film ring (3) is circular, and microporous filter membrane (2) is also
Circular.
Further, cup annular groove (4) cross section on described cup (1) lower surface is semicircle, corresponding micropore filter
Upper fixing dress film ring (3) cross section of film (2) is semicircle or circular.
Further, described cup (1) lower end is provided with the junction (103) that inward wall thickening extends, and junction
(103) downward-sloping pyramidal structure is become.
Further, described cup (1) be cross section be polygonal cavity stereochemical structure, corresponding dress film ring (3) is
Corresponding polygonized structure, microporous filter membrane (2) is also corresponding polygonized structure.
The using method when present invention uses said apparatus to operate, a kind of microporous filter membrane pelletizer is in film-making and shows
Assembly method when micro mirror is observed, it includes film-making and microexamination two parts, first step film-making: by fixing for dress film ring (3) dress
Fit over microporous filter membrane (2) upper surface, and the cup on cup (1) end face will be arranged on the microporous filter membrane (2) of dress film ring (3)
In annular groove (4), i.e. form the device as described in any one of claim 1-5；Second step microexamination: treat cup (1) interior work
After work, dress film ring (3) with microporous filter membrane (2) is taken off, reversion microporous filter membrane (2), be allowed to be arranged on band fluted
(501) microexamination is carried out on micro slide (5).
Further, microporous filter membrane (2) is affixed directly to fill film ring (3) side, and dress film ring (3) sticking veneer is plane.
Further, cup (1) bottom is provided with cup annular groove (4), and dress film ring (3) with microporous filter membrane (2) is permissible
It is directly clamped among in the cup annular groove (4) matched.
Further, the bottom outside of cup (1) is provided with and takes ring plate mouth (104), and dress film ring (3) is exposed to cup (1)
Outside, can stir dress film ring (3) with tweezers and be taken off by dress film ring (3) with microporous filter membrane (2) completing work.
Further, observe in order to matching film plate is directly used in microscope, devise a kind of special micro slide (6), loading
Sheet (6) is made of clear material, and devises the groove (501) that shape is coincide with dress film ring (3) shape, by microporous filter membrane
(2) sightingpiston is close to microscope observation platform (6) down.
Beneficial effects of the present invention: the invention provides a kind of microporous filter membrane pelletizer and see in film-making and microscope
Assembly method when examining, have employed a kind of brand-new structure and film-making, microscopy method, conveniently carries out taking off after microporous filter membrane film-making
From, and fast and easy carries out fixed mirror inspection on micro slide.
1, the separation after fast and easy carries out film-making, and film-making.
2, during observing, it is not necessary to again separate microporous filter membrane, it is to avoid microporous filter membrane crushes, and facilitates microscopy to operate, letter
Change operation sequence, save the operating time.
Accompanying drawing explanation
Fig. 1 is cup of the present invention and microporous filter membrane combinative structure schematic diagram.
Fig. 2 is A-A cross-sectional schematic in Fig. 1.
Fig. 3 is another kind structure A-A cross-sectional schematic of the present invention in Fig. 1.
Fig. 4 is the microporous filter membrane of the present invention scheme of installation on micro slide.
Described in figure, label character is expressed as: 1, cup；2, microporous filter membrane；3, dress film ring；4, cup annular groove；5, loading
Sheet；6, microscope observation platform；101, upper shed；102, under shed；103, junction；104, ring plate mouth is taken；501, groove.
Detailed description of the invention
In order to make those skilled in the art be more fully understood that technical scheme, below in conjunction with the accompanying drawings the present invention is entered
Row describes in detail, and the description of this part is only exemplary and explanatory, should not have any restriction to protection scope of the present invention
Effect.
As Figure 1-Figure 4, the concrete structure of the present invention a kind of microporous filter membrane pelletizer is, it include cup 1 and
Microporous filter membrane 2 bottom it is set, described cup 1 lower surface is provided with cup annular groove 4, and microporous filter membrane 2 surface is fixed
Having the dress film ring 3 corresponding with cup annular groove 4, the dress film ring 3 on microporous filter membrane 4 is freely embeddable in cup annular groove 4；And
It is provided with in the bottom of cup 1 and takes ring plate mouth 104.
Preferably, described cup 1 is revolving body, and corresponding dress film ring 3 is circular, and microporous filter membrane 2 is also circular.
Preferably, cup annular groove 4 cross section on described cup 1 lower surface is semicircle, solid on corresponding microporous filter membrane 2
Fixed dress film ring 3 cross section is semicircle or circular.
Preferably, described cup 1 lower end is provided with inward wall and thickeies the junction 103 extended, and junction 103 becomes downward
The pyramidal structure tilted.
Preferably, described cup 1 is polygonal cavity stereochemical structure for cross section, and corresponding dress film ring 3 is many for correspondence
Limit shape structure, microporous filter membrane 2 is also corresponding polygonized structure.
Time specifically used, liquid enters from the upper shed 101 of cup 1, and the microporous filter membrane 2 arranged by lower end is cut off, and enters
Row filters, and filtrate is discharged under shed 102, and the material in liquid is retained on microporous filter membrane 2 and treats that next step operates.
The using method when present invention uses said apparatus to operate, a kind of microporous filter membrane pelletizer is in film-making and shows
Assembly method when micro mirror is observed, it includes film-making and microexamination two parts, first step film-making: by fixing for dress film ring 3 assembling
At microporous filter membrane 2 upper surface, and will be arranged in the cup annular groove 4 on cup 1 end face with the microporous filter membrane 2 of dress film ring 3,
I.e. form the device as described in any one of claim 1-5；Second step microexamination: treating in cup 1 after hours will be with
The dress film ring 3 of microporous filter membrane 2 takes off, and inverts microporous filter membrane 2, is allowed to be arranged on carrying out on the micro slide 5 of fluted 501 micro-
Observe.
Preferably, microporous filter membrane 2 is affixed directly to fill film ring 3 side, and dress film ring 3 sticking veneer is plane.
Preferably, bottom cup 1, be provided with cup annular groove 4, with the dress film ring 3 of microporous filter membrane 2 can be directly clamped among with
Coupling cup annular groove 4 in.
Preferably, the bottom outside of cup 1 is provided with and takes ring plate mouth 104, and dress film ring 3 is exposed to outside cup 1, Ke Yiyong
Tweezers are stirred dress film ring 3 and are taken off by the dress film ring 3 with microporous filter membrane 2 completing work.
Preferably, in order to matching film plate be directly used in microscope observe, devise a kind of special micro slide 6, micro slide 6 by
Transparent material is made, and devises the groove 501 that shape is coincide with dress film ring 3 shape, by the tightest for microporous filter membrane 2 sightingpiston
Patch microscope observation platform 6.
It should be noted that in this article, term " includes ", " comprising " or its any other variant are intended to non-row
Comprising of his property, so that include that the process of a series of key element, method, article or equipment not only include those key elements, and
And also include other key elements being not expressly set out, or also include intrinsic for this process, method, article or equipment
Key element.
Principle and the embodiment of the present invention are set forth by specific case used herein, the explanation of above example
It is only intended to help to understand method and the core concept thereof of the present invention.The above is only the preferred embodiment of the present invention, should
When pointing out, due to the finiteness of literal expression, and objectively there is unlimited concrete structure, for the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, it is also possible to make some improvement, retouch or change, it is also possible to will
Above-mentioned technical characteristic is combined by rights；These improve retouching, change or combine, or the most improved structure by invention
Think and technical scheme directly applies to other occasion, be regarded as protection scope of the present invention.
Claims (10)
1. a microporous filter membrane pelletizer, it includes cup (1) and arranges the microporous filter membrane (2) bottom it, and its feature exists
In, described cup (1) lower surface is provided with cup annular groove (4), and microporous filter membrane (2) surface is fixed with and cup annular groove
(4) corresponding dress film ring (3), dress film ring (3) on microporous filter membrane (4) is freely embeddable in cup annular groove (4)；And at cup
The bottom of body (1) is provided with and takes ring plate mouth (104).
A kind of microporous filter membrane pelletizer the most according to claim 1, it is characterised in that described cup (1) is revolving body,
Corresponding dress film ring (3) is circular, and microporous filter membrane (2) is also circular.
A kind of microporous filter membrane pelletizer the most according to claim 1, it is characterised in that on described cup (1) lower surface
Cup annular groove (4) cross section be semicircle, upper fixing dress film ring (3) cross section of corresponding microporous filter membrane (2) be semicircle or
Person is circular.
A kind of microporous filter membrane pelletizer the most according to claim 1, it is characterised in that described cup (1) lower end is arranged
The junction (103) that oriented oncus extends, and junction (103) become downward-sloping pyramidal structure.
A kind of microporous filter membrane pelletizer the most according to claim 1, it is characterised in that described cup (1) is cross section
For polygonal cavity stereochemical structure, corresponding dress film ring (3) is corresponding polygonized structure, and microporous filter membrane (2) is also many for correspondence
Limit shape structure.
6. the microporous filter membrane pelletizer assembly method when film-making and microscope are observed, it is characterised in that it includes system
Sheet and microexamination two parts, first step film-making: dress film ring (3) is securely fitted in microporous filter membrane (2) upper surface, and will be with
The microporous filter membrane (2) of dress film ring (3) is arranged on the cup annular groove (4) on cup (1) end face Nei, is i.e. formed such as claim 1-
Device described in 5 any one；Second step microscope is observed: after hours will be with the dress of microporous filter membrane (2) in treating cup (1)
Film ring (3) takes off, reversion microporous filter membrane (2), is allowed to be arranged on and carries out microscope sight with on the micro slide (5) of fluted (501)
Examine.
A kind of microporous filter membrane pelletizer the most according to claim 6 assembly method when film-making and microscope are observed,
It is characterized in that, microporous filter membrane (2) is affixed directly to fill film ring (3) side, and dress film ring (3) sticking veneer is plane.
A kind of microporous filter membrane pelletizer the most according to claim 6 assembly method when film-making and microscope are observed,
It is characterized in that, cup (1) bottom is provided with cup annular groove (4), and dress film ring (3) with microporous filter membrane (2) can directly block
In the cup annular groove (4) matched.
A kind of microporous filter membrane pelletizer the most according to claim 6 assembly method when film-making and microscope are observed,
It is characterized in that, the bottom outside of cup (1) is provided with and takes ring plate mouth (104), and dress film ring (3) is exposed to cup (1) outside, can
With with tweezers stir dress film ring (3) dress film ring (3) with microporous filter membrane (2) completing work is taken off.
A kind of microporous filter membrane pelletizer the most according to claim 6 assembly method when film-making and microscope are observed,
It is characterized in that, in order to matching film plate be directly used in microscope observe, devise a kind of special micro slide (6), micro slide (6) by
Transparent material is made, and devises the groove (501) that shape is coincide with dress film ring (3) shape, by microporous filter membrane (2) sightingpiston
It is close to microscope observation platform (6) down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610645966.XA CN106289906B (en) | 2016-08-08 | 2016-08-08 | Microporous filter membrane tabletting device and assembling method thereof during tabletting and microscopic observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610645966.XA CN106289906B (en) | 2016-08-08 | 2016-08-08 | Microporous filter membrane tabletting device and assembling method thereof during tabletting and microscopic observation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106289906A true CN106289906A (en) | 2017-01-04 |
| CN106289906B CN106289906B (en) | 2024-02-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610645966.XA Active CN106289906B (en) | 2016-08-08 | 2016-08-08 | Microporous filter membrane tabletting device and assembling method thereof during tabletting and microscopic observation |
Country Status (1)
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| CN (1) | CN106289906B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108693008A (en) * | 2018-07-25 | 2018-10-23 | 湖南省天骑医学新技术股份有限公司 | A kind of miillpore filter cup collection device for miillpore filter transfer |
| CN109443881A (en) * | 2018-12-24 | 2019-03-08 | 湖南省天骑医学新技术股份有限公司 | A kind of miillpore filter fast-collection device |
| CN109459289A (en) * | 2018-12-24 | 2019-03-12 | 湖南省天骑医学新技术股份有限公司 | A kind of quick pelletizer |
| CN109554285A (en) * | 2018-12-29 | 2019-04-02 | 北京大学 | A kind of flexible microporous film and the cell separation apparatus using its preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108693008A (en) * | 2018-07-25 | 2018-10-23 | 湖南省天骑医学新技术股份有限公司 | A kind of miillpore filter cup collection device for miillpore filter transfer |
| CN108693008B (en) * | 2018-07-25 | 2024-01-30 | 湖南省天骑医学新技术股份有限公司 | A millipore filter cup collection device for millipore filter shifts |
| CN109443881A (en) * | 2018-12-24 | 2019-03-08 | 湖南省天骑医学新技术股份有限公司 | A kind of miillpore filter fast-collection device |
| CN109459289A (en) * | 2018-12-24 | 2019-03-12 | 湖南省天骑医学新技术股份有限公司 | A kind of quick pelletizer |
| CN109443881B (en) * | 2018-12-24 | 2024-01-30 | 湖南省天骑医学新技术股份有限公司 | Quick collection device of millipore filter membrane |
| CN109554285A (en) * | 2018-12-29 | 2019-04-02 | 北京大学 | A kind of flexible microporous film and the cell separation apparatus using its preparation |
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| Publication number | Publication date |
|---|---|
| CN106289906B (en) | 2024-02-23 |
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