CN205501299U - Catch unit and separator - Google Patents
Catch unit and separator Download PDFInfo
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- CN205501299U CN205501299U CN201620130839.1U CN201620130839U CN205501299U CN 205501299 U CN205501299 U CN 205501299U CN 201620130839 U CN201620130839 U CN 201620130839U CN 205501299 U CN205501299 U CN 205501299U
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- cavity
- separator
- capturing unit
- cell
- circulation
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Abstract
The utility model provides a catch the unit for filter the biological fluid in order to catch the rare cell of circulation, catch the millipore filtration that the unit includes filterable biological fluid, connect in millipore filtration polymer substance layer on the surface, and locate the polymer substance in situ by can with wait to catch the layer of catching that biomolecule that the rare cell of circulation carries out specifically bind to constitutes, catch the unit and can simplify the separation of the rare cell that circulates, catch the operation, obtain simultaneously to the high score of the rare cell that circulates from, capture rate, and the high -purity of the rare cell of circulation of catching. The utility model also provides an including the separator of catching the unit, separator simple structure, easily operation to do benefit to going on of follow -up appraisal detection.
Description
Technical field
This utility model relates to cell in vitro separation technology field, particularly to a kind of capturing unit, meanwhile,
Further relate to the separator being made up of this capturing unit.
Background technology
Circulation rare cell in human peripheral includes circulating tumor cell (CTCs), circulating stem cell (CSCs)
With the circulation fetal cell multiple cell with critical biological meaning and clinical value such as (CFCs), the most right
The research of circulating tumor cell is more, and circulating tumor cell refers to spontaneous or because operation of diagnosis and treatment is broadcast by tumor focus
Dissipate the tumor cell entering Peripheral Circulation, from blood, therefore detect circulating tumor cell increasingly cause people
Attention.But, circulating tumor cell content in peripheral blood is few, may be only in every 10mL blood
Containing several to dozens of circulating tumor cells, but it is up to about 100,000,000 leukocyte and 50,000,000,000 erythrocyte,
Under the interference of the background cells of enormous amount, it is impossible to circulating tumor cell is directly included gene mutation,
The detection of surface marker, cytoactive, secretory protein spectrum and mechanical property etc., so by circulating tumor
It is the necessary step before detecting that cell is separated from peripheral blood, but currently used separation equipment is grasped mostly
Make complexity, there is the separation rate to circulating tumor cell and separation purity is low, or the circulating tumor cell separated
It is unfavorable for the problems such as follow-up release detection.
Utility model content
In view of this, this utility model is directed to a kind of capturing unit to circulation rare cell, it is possible to letter
Change to circulation rare cell separation, capture operation, obtain simultaneously to circulation rare cell high score from, catch
Obtain efficiency, and the high-purity of the circulation rare cell of capture.
For reaching above-mentioned purpose, the technical solution of the utility model is achieved in that
A kind of capturing unit, for filtered biological fluid with capture circulation rare cell, described capturing unit bag
Include the microporous filter membrane that may filter that biofluid, the polymer substance layer being connected on described microporous membrane surface,
And it is interior by carrying out specific binding life with circulation rare cell to be captured to be located at described polymer substance layer
The trapping layer of thing molecular composition.
Described circulation rare cell includes that circulating tumor cell, circulating stem cell or circulation fetal cell etc. exist
In organism peripheral blood, rare numbers, but there is the cell of critical biological meaning and clinical value.
Further, described can carry out specific binding biomolecule with circulation rare cell to be captured and include
Antibody, folic acid or nucleotide.
Further, the aperture of described microporous filter membrane is 10～30 μm.
Further, the thickness of described polymer substance layer is 1～5 μm.
Relative to prior art, this utility model has the advantage that
(1) capturing unit described in the utility model includes the micropore for filtering blood or other biofluid
Filter membrane, and the polymer substance layer consisted of chemical coupling polymer substance at microporous membrane surface, such as water
Gel etc., and via polymer substance connect with circulation rare cell (i.e. target cell) can specificity
In conjunction with the biomolecule such as biomolecule such as antibody, folic acid or nucleotide constitute trapping layer.Work as blood sample
Or other biofluid is when flowing through capturing unit, the circulation rare cell in biofluid and antibody or nucleotide
Produce specific binding, be fixed on microporous membrane surface, other cell in biofluid, as erythrocyte,
Leukocyte etc., can be by the micropore on filter membrane, along with biofluid together flows out, thus realizes circulation dilute
There are the separation of cell, capture.And capture circulation rare cell microporous filter membrane can directly carry out cell fix,
The follow-up identification and detection such as dyeing, microscope observation, do not interfere with qualification result.
(2) aperture of microporous filter membrane and the thickness of polymer substance layer in capturing unit described in the utility model
Can affect the circulation separation of rare cell, capture rate, and the circulation rare cell purity of capture, pass through
The restriction of above-mentioned parameter, to improve the capture effect of capturing unit.
Another object of the present utility model is to propose a kind of separator, is beneficial to divide circulation rare cell
From capture operation.
For reaching above-mentioned purpose, the technical solution of the utility model is achieved in that
A kind of separator, including capturing unit as above.
Further, described separator also includes inner hollow, and two ends are provided with the cavity of opening, described capture
Unit is arranged in described cavity
Further, described cavity is by making by pressure-bearing material.
Further, described cavity includes separable first cavity and the second cavity, and described capturing unit sets
It is placed in described first cavity or/and in the second cavity.
Relative to prior art, this utility model has the advantage that
(1) separator described in the utility model includes capturing unit as above, relies primarily on capture single
Unit carries out separating trap to circulation rare cell, and described separator can use the shape of any existing separator
Formula, such as various filter etc., beneficially separating trap operation.
(2) capturing unit is arranged at inner hollow by separator described in the utility model, and two ends are provided with opening
Cavity in, can control biofluid slowly in cavity circulate, effectively slow down the speed flowing through capturing unit,
Improve the capture effect of capturing unit.Described cavity, by making by pressure-bearing material, can bear for coutroi velocity
On-load pressure, to improve capture effect further.Additionally, further cavity to be set to separable two
Part, is beneficial to carry out follow-up identification and detection process to after circulation rare cell separating trap.After separation completes,
First cavity and the second cavity are opened, carry out in each several part cavity with capturing unit cell fix,
Dyeing processes, and finally takes out capturing unit and carries out observing, determining qualification result.After having detected the most desirable new
Capturing unit is placed in each several part cavity again, Reusability.Above-mentioned cyclone separator arrangement is simple, except being beneficial to separate,
The operation of capture circulation rare cell, and have outside high capture rate, and be beneficial to circulation rare cell
Follow-up identification and detection.
Accompanying drawing explanation
The accompanying drawing constituting a part of the present utility model is further appreciated by of the present utility model for providing, this
The schematic description and description of utility model is used for explaining this utility model, is not intended that this practicality new
The improper restriction of type.In the accompanying drawings:
Fig. 1 is the cyclone separator arrangement schematic diagram described in this utility model embodiment；
Fig. 2 is the capturing unit structural representation described in this utility model embodiment；
Fig. 3 is the use view of structure shown in Fig. 2；
Description of reference numerals:
1-1, the first cavity, 1-2, the second cavity, 2, connector, 3-1, the first opening, 3-2, second
Opening, 4, capturing unit, 5, microporous filter membrane, 6, polymer substance layer, 7, trapping layer, 8, target cell.
Detailed description of the invention
It should be noted that in the case of not conflicting, in embodiment in this utility model and embodiment
Feature can be mutually combined.
Describe this utility model below with reference to the accompanying drawings and in conjunction with the embodiments in detail.
Embodiment
The present embodiment relates to a kind of separator, as it is shown in figure 1, described separator includes the cavity of inner hollow,
Described cavity includes the first cavity 1-1 and two parts that can be kept completely separate of the second cavity 1-2, described first
Cavity 1-1 and the second cavity 1-2 is combined as the overall structure of cavity 1 by connector 2.At described cavity 1
Two ends be provided with opening, i.e. one end at the first cavity 1-1 arranges the first opening 3-1, at the second cavity 1-2
One end the second opening 3-2 is set, be used for circulate blood sample or other biofluid.Set in described cavity
Put capturing unit 4, for the circulation separation of rare cell, capture.
The structure of described capturing unit 4 is as in figure 2 it is shown, include for filtering blood or other biofluid
Microporous filter membrane 5, connects on the surface of described microporous filter membrane 5 and has a floor height molecular substance layer 6, at polymer
Connect in matter layer 6 and have and can carry out specific binding antibody or folic acid or nucleotide with circulation rare cell
The trapping layer 7 constituted.The aperture of described microporous filter membrane 5 is preferably 10～30 μm, such as 15 μm, 25 μm；
The thickness of described polymer substance layer 6 is 1～5 μm, such as 3 μm, 5 μm；Be conducive to raising rare to circulation
The capture effect of cell.
When using above-mentioned separator that circulation rare cell is separated, blood sample will be filled or other will be biological
The container of fluid is connected to the first opening 3-1 by pipeline, is connected to the second opening 3-2 flow out pipeline, makes
Blood sample or other biofluid slowly flow through in separator, it is also possible to by pressurizeing pipeline,
Control circulating rate, improve capture effect.For bearing pressure, cavity can by rustless steel, politef,
Pottery etc. can carry the material of certain pressure and make.When blood sample or other biofluid flow through in separator
Capturing unit 4 time, circulation rare cell and antibody or nucleotide generation are specific binding, are fixed on micro-
Hole filter membrane surface, as it is shown on figure 3, capturing unit 4 captures target cell 8 (i.e. circulating rare cell), its
Its cell, such as erythrocyte, leukocyte etc., can pass through the micropore on filter membrane, along with biofluid together flows out,
Thus realize the separating trap to circulation rare cell.After completing to separate, open connector 2, with catching
Carry out in obtaining each several part cavity of unit 4 cell fix, dyeing process, finally take out microporous filter membrane observe,
Qualification result, the hole existed due to microporous filter membrane does not affect processing procedure, is identified the most accurately
Result.Take new capturing unit 4 after having detected to be placed in again in each several part cavity, be re-used for separating trap
Operation.
In the structure of above-mentioned separator, connector 2 can be threaded, it is also possible to connects for eye-splice, or
Other type of attachment.One group of capturing unit 4 can be set in described cavity, be placed in the first cavity 1-1 or second
In cavity 1-2, it is also possible to many group capturing units 4 are set, are respectively placed in the first cavity 1-1 and the second cavity
In 1-2.
Additionally, separator of the present utility model can be to use existing any filtration, the form of separator, will
Capturing unit described in the utility model is arranged in separator, as being arranged in film plate type filter.
The foregoing is only preferred embodiment of the present utility model, not in order to limit this utility model,
All within spirit of the present utility model and principle, any modification, equivalent substitution and improvement etc. made, all
Within protection domain of the present utility model should be included in.
Claims (8)
1. a capturing unit, for filtered biological fluid with capture circulation rare cell, it is characterised in that:
Described capturing unit includes the microporous filter membrane that may filter that biofluid, is connected on described microporous membrane surface
Polymer substance layer, and it is interior by carrying out with circulation rare cell to be captured to be located at described polymer substance layer
The trapping layer that specific binding biomolecule is constituted.
Capturing unit the most according to claim 1, it is characterised in that: described can be dilute with circulation to be captured
There is cell to carry out specific binding biomolecule and include antibody, folic acid or nucleotide.
Capturing unit the most according to claim 1, it is characterised in that: the aperture of described microporous filter membrane is
10～30 μm.
Capturing unit the most according to claim 1, it is characterised in that: the thickness of described polymer substance layer
Degree is 1～5m.
5. a separator, it is characterised in that: described separator includes capturing as claimed in claim 1 list
Unit.
Separator the most according to claim 5, it is characterised in that: described separator also includes in inside
Sky, two ends are provided with the cavity of opening, and described capturing unit is arranged in described cavity.
Separator the most according to claim 5, it is characterised in that: described cavity is by can pressure-bearing material system
Become.
8. according to the separator described in claim 5 or 6, it is characterised in that: described cavity includes separable
The first cavity and the second cavity, described capturing unit is arranged at described first cavity or/and in the second cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620130839.1U CN205501299U (en) | 2016-02-19 | 2016-02-19 | Catch unit and separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620130839.1U CN205501299U (en) | 2016-02-19 | 2016-02-19 | Catch unit and separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205501299U true CN205501299U (en) | 2016-08-24 |
Family
ID=56724616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620130839.1U Active CN205501299U (en) | 2016-02-19 | 2016-02-19 | Catch unit and separator |
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| CN (1) | CN205501299U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635769A (en) * | 2016-11-07 | 2017-05-10 | 深圳市达科为生物工程有限公司 | Device and method for separating cells |
| CN107338185A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | The catching method of biomolecule in a kind of cell or solution |
| CN108392232A (en) * | 2018-04-11 | 2018-08-14 | 苏州大学 | Using functionalization albumen silk thread as the internal cell capture device of carrier |
| WO2019023960A1 (en) * | 2017-08-02 | 2019-02-07 | Suzhou Bofu Biomedical Limited | Functionalized mesh and fluidic apparatus for capturing cells or molecules in solution |
| WO2019023961A1 (en) * | 2017-08-02 | 2019-02-07 | Suzhou Bofu Biomedical Limited | Method for capturing target cells or molecules in solution |
-
2016
- 2016-02-19 CN CN201620130839.1U patent/CN205501299U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635769A (en) * | 2016-11-07 | 2017-05-10 | 深圳市达科为生物工程有限公司 | Device and method for separating cells |
| CN106635769B (en) * | 2016-11-07 | 2019-06-28 | 深圳市达科为生物工程有限公司 | Cell separation apparatus and cell isolation method |
| CN107338185A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | The catching method of biomolecule in a kind of cell or solution |
| WO2019023960A1 (en) * | 2017-08-02 | 2019-02-07 | Suzhou Bofu Biomedical Limited | Functionalized mesh and fluidic apparatus for capturing cells or molecules in solution |
| WO2019023961A1 (en) * | 2017-08-02 | 2019-02-07 | Suzhou Bofu Biomedical Limited | Method for capturing target cells or molecules in solution |
| US11525829B2 (en) | 2017-08-02 | 2022-12-13 | Hemosmart Medical Technology Ltd. | Method for capturing target cells or molecules in solution |
| CN108392232A (en) * | 2018-04-11 | 2018-08-14 | 苏州大学 | Using functionalization albumen silk thread as the internal cell capture device of carrier |
| CN108392232B (en) * | 2018-04-11 | 2023-07-25 | 苏州大学 | In vivo cell capturing device using functional protein silk thread as carrier |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |