CN108392232A - Using functionalization albumen silk thread as the internal cell capture device of carrier - Google Patents
Using functionalization albumen silk thread as the internal cell capture device of carrier Download PDFInfo
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- CN108392232A CN108392232A CN201810321288.0A CN201810321288A CN108392232A CN 108392232 A CN108392232 A CN 108392232A CN 201810321288 A CN201810321288 A CN 201810321288A CN 108392232 A CN108392232 A CN 108392232A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention provides a kind of using functionalization albumen silk thread as the internal cell capture device of carrier, it include the internal cell capture pipe using functionalization albumen silk thread as carrier, internal cell capture pipe includes a fixing pipe, several root functionality albumen silk threads and the cell capture ligand being fixed on functionalization albumen silk thread, one end of functionalization albumen silk thread is fixed in fixing pipe, the outer cover of fixing pipe is equipped with guide pin, sealing-plug is fixedly connected in guide pin, sealing-plug is surrounded on the outside of fixing pipe, sealing-plug, fixing pipe surrounds the cavity of one end band opening with guide pin, the other end of functionalization albumen silk thread is within the cavity, fixing pipe is also associated with conducting wire pole, conducting wire pole is located in the guide pin and can opposite sliding with guide pin.The grabber good biocompatibility of the present invention is easy to contact with target cell, improves the capture rate of characteristic cell in vivo.
Description
Technical field
It a kind of catches the present invention relates to medical instruments field more particularly to by the internal cell of carrier of functionalization albumen silk thread
Obtain device.
Background technology
There is important clinical value, especially circulating tumor with the relevant characteristic cell of disease in blood of human body
Cell (CTC).However, the quantity of characteristic cell (especially CTC) in blood is extremely rare, it is rare cell, usually examines
Survey method includes the process of cell capture and enrichment.Catching method is divided into vitro and in vivo, the former is due to detecting sample
Blood volume it is less, be susceptible to false negative.Just because of the reason, some internal cell capture methods are developed now.Nothing
By being internal or external, typically by specific capture ligands (such as epithelial cell adhesion molecule antibody, the nucleic acid of target cell
Aptamer, polypeptide aptamer) it is immobilized on a carrier, to build cell capture device, then this grabber is placed in blood vessel and captures height
The target cell of speed flowing.
Currently, in vivo carrier used in cell capture device include chemical fibre silk, it is organic high molecular polymer line, medical
Remaining needle, medical stainless steel silk and ductus venosus.However, the internal cell capture device with these vector constructions is clearly present four
The deficiency of aspect.First, in order to resist the high speed impact of venous blood, it is often necessary to the diameter of larger (500 microns or more), with
Silk thread is avoided to be broken and set and stay in blood vessel；Second, large-diameter material rigidity reinforced, with flexible blood vessel high speed flow target cell
Contact probability will greatly reduce；Third, under the premise of not influencing blood vessel normal function, major diameter carrier material is narrow
The grabber containing more carrier lines (or pipe) can not possibly be placed in vein blood vessel, in fact, being to be with solid wire (or pipe)
The grabber of carrier material structure, grabber have minimum specific surface area, this also leads to swiftly flowing target cell
It is greatly reduced with the contact probability of grabber；4th, although these carrier material good biocompatibility, small toxicities, they
Cannot be biodegradable, once occurring being broken and setting and stay in blood vessel, serious medical care problem will be caused.
Invention content
In order to solve the above technical problems, the object of the present invention is to provide a kind of using functionalization albumen silk thread as the internal of carrier
Cell capture device, not only good biocompatibility, and can be biodegradable, it is easy to contact with target cell, it is thin to improve feature
The capture rate of born of the same parents in vivo.
The present invention provides a kind of using functionalization albumen silk thread as the internal cell capture device of carrier, including with functionalization egg
White silk thread is the internal cell capture pipe of carrier, and internal cell capture pipe includes a fixing pipe, several functionalization eggs flexible
White silk thread and the cell capture ligand being fixed on functionalization albumen silk thread, cell capture ligand is for capturing cell, function
The one end for changing albumen silk thread is fixed in fixing pipe, and the other end of functionalization albumen silk thread can be relatively fixed pipe freely activity, Gu
The outer cover of fixed tube is equipped with guide pin, and sealing-plug is fixedly connected in guide pin, and sealing-plug is surrounded on the outside of fixing pipe, and sealing-plug is consolidated
Fixed tube surrounds the cavity of one end band opening with guide pin, and the other end of functionalization albumen silk thread is within the cavity, and fixing pipe is also connected with
There are conducting wire pole, conducting wire pole to be located in the guide pin and can opposite sliding with guide pin.
Further, functionalization albumen silk thread is 2-60 roots.Fix more root functionality albumen simultaneously in cell capture pipe
Silk thread substantially increases the specific surface area of grabber, is also obviously increased with the probability that cell to be captured contacts in blood, makes thin
The capture rate of born of the same parents significantly improves, and realizes high efficiency cell capture.
Further, functionalization albumen silk thread includes albumen silk thread and the function object being fixedly connected with albumen wire surface
Matter, functional mass connect cell capture ligand.
Further, albumen silk thread is mulberry silk, tussah silk or spider silk, and functional mass is the chemical combination containing functional group
The nanoparticle of object or compound and functionalization containing functional group, cell capture ligand are specific antibody (such as epithelial cell
Adhesion molecule antibody), one or more of specific nucleic acid aptamer and polypeptide aptamer.
Further, the compound containing functional group is contains epoxides, carboxylated compound and chemical combination containing amino
One or more of object.
Further, contains epoxides are diglycidyl ether, such as polyethyleneglycol diglycidylether and/or Isosorbide-5-Nitrae-
Butanediol diglycidyl ether.
Further, carboxylated compound is polyacrylic acid (PAA), acrylic acid and methyl acrylate copolymer, acrylic acid
With ethyl acrylate copolymer or the polymer of polyacrylic acid part acetyl amination.
Further, it is polyamine compounds to contain amino-compound, such as ethylenediamine, propane diamine, butanediamine and polyetherimide
One or more of (PEI).
Further, the nanoparticle of functionalization is one kind in silica dioxide nano particle and titanium dioxide nano granule.
Further, the preparation method for being fixed with the functionalization albumen silk thread of cell capture ligand includes the following steps：
Albumen silk thread and the compound containing functional group or the nanoparticle of the compound containing functional group and functionalization are existed
6h is reacted at 60 DEG C, then reacts 3h at 37 DEG C with cell capture ligand under the action of crosslinking agent, is added after alkanamine instead
Answer 4h.
Further, crosslinking agent is 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides (EDC) and N- hydroxyls
Succinimide (NHS).
Further, one end of functionalization albumen silk thread is fixed on by hydrogel in fixing pipe.Hydrogel is that crosslinking is poly-
Acrylamide gel.
Further, fixing pipe is hose, and material is high molecular polymer, such as polytetrafluoroethylene (PTFE), PVC, ethylene fluoride
Propylene copolymer (FEP) or polyurethane.
Further, cell is the tire source property cell in circulating tumor cell (CTC) or Disease in Infants blood.
Further, the one end of guide pin far from conducting wire pole is removably connected with liquid storage unit, and liquid storage unit includes liquid storage
Set, sealing cover and the preservation liquid in liquid storage set, offer through-hole on sealing cover, guide pin be arranged in through-hole and with liquid storage set
Connection, cavity are interior filled with preservation liquid.It preserves in the cavity where liquid is filled in liquid storage set and functionalization albumen silk thread so that work(
The capture ligands that can change on albumen silk thread will not inactivate during preservation.
Further, the inner wall of guide pin is also fixedly connected with multiple guiding plugs.
Further, it is two to be oriented to plug number, one of them is surrounded on the outside of fixing pipe, another is surrounded on seal wire
On the outside of bar.
Further, guide pin external stability is connected with the guide pin handle of sheet, the one end of conducting wire pole far from the fixing pipe
It is connected with the seal wire handle of sheet, position line is connected between guide pin handle and seal wire handle.
Further, aperture is offered on guide pin handle and seal wire handle, the both ends of position line are arranged in aperture simultaneously
It is fixedly connected respectively with guide pin handle and seal wire handle.The setting of position line enables to conducting wire pole in sliding opposite with guide pin
When, one end will not be skidded off out of guide pin.
Further, the material of guide pin handle and seal wire handle is elastic material.It is convenient to fix when in use on the skin.
Further, guide pin handle and seal wire handle are equipped with several anti-skidding salient points.
According to the above aspect of the present invention, the present invention has at least the following advantages：
Internal cell capture pipe in the internal cell capture device of the present invention is using functionalization albumen silk thread as carrier, Protein filament
Line has extremely strong tensile strength, while also having preferable toughness, is fully able to resist the high speed impact of venous blood.And
And the diameter of albumen silk thread is very small, and usually 10-15 μm or so, the 1/30 of only conventional capture silk thread, sometimes even 1/
50.The diameter of native protein silk thread is suitable with cell dia to be captured, advantageously reduces the steric hindrance of capturing carrier.Meanwhile
Superfine albumen silk thread macroscopically also shows apparent flexibility, in swiftly flowing blood, these soft silk threads
By high speed swinging, it is made to be come into full contact with cell to be captured, difficult ask is contacted to overcome rigid carrier with target cell
Topic.Since albumen silk thread is superfine, several capture silk threads can be fixed simultaneously in cell capture pipe, this grabber is placed in vein
Also it is unlikely to constitute apparent influence to the proper flow of blood in blood vessel, the grabber thus constituted is substantially increased and caught
The specific surface area for obtaining device also obviously increases with the probability that cell to be captured contacts in blood, the capture rate of cell is made to significantly improve,
Realize high efficiency cell capture.Since the raw material of functionalization albumen silk thread are native proteins, there is fabulous biofacies
Capacitive and good biodegradability, if just in case breakage problem occur, it both can be as current blood vessel retention material such as indwelling
Needle, inlying catheter use the method that clinical operation takes out, and voluntarily biodegradable method may be used, this greatly reduces
The risk that capture thread breakage constitutes human body.
Above description is only the general introduction of technical solution of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention and after coordinating attached drawing to be described in detail such as.
Description of the drawings
Fig. 1 is the structural schematic diagram of cell capture device in vivo of the invention；
Fig. 2 is the enlarged drawing at Fig. 1 centre circles A；
Fig. 3 is the selectivity result that cell capture device of the present invention captures CTC with control group；
Fig. 4 is the microphoto of the CTC of control group capture；
Fig. 5 is the microphoto of the CTC of cell capture device capture of the present invention；
Fig. 6 is the functionalization albumen silk thread and CTC capture number relationships that different radicals are fixed in cell capture device of the present invention
Test result figure；
Fig. 7 is the stereoscan photograph of fixed functionalization albumen silk thread in cell capture device of the present invention；
Fig. 8 is the scanning electricity of the immobilized silicon nanoparticle of fixed functionalization albumen wire surface in cell capture device of the present invention
Mirror photo；
Fig. 9 is Toxic test results of the different material to cell；
Figure 10 is the hemolytic experiment test result of different material；
Figure 11 is the hemagglutinin experiment survey of the functionalization albumen silk thread in cell capture device of the present invention and medical stainless steel silk
Test result；
Reference sign：
1- liquid storage sets；2- guide pins；3- albumen silk threads；4- sealing-plugs；5- is oriented to plug；6- guide pin handles；7- position lines；8- is caught
Obtain ligand；9- fixing pipes；10- hydrogels；11- conducting wire poles；12- seal wire handles；13- preserves liquid；14- sealing covers；15- is anti-skidding convex
Point.
Specific implementation mode
With reference to the accompanying drawings and examples, the specific implementation mode of the present invention is described in further detail.Implement below
Example is not limited to the scope of the present invention for illustrating the present invention.
Embodiment 1
Referring to Fig. 1-2, the present embodiment preferred embodiment it is a kind of using functionalization albumen silk thread as the internal CTC cells of carrier
Grabber, including internal CTC cell captures pipe, internal cell CTC capture ducts include a fixing pipe 9 and several root functionality eggs
White silk thread, preferably 40.Fixing pipe 9 is hose, and material is high molecular polymer, such as polytetrafluoroethylene (PTFE), PVC, FEP or poly-
Urethane.One end of functionalization albumen silk thread is fixedly connected on by hydrogel 10 in fixing pipe 9, and functionalization albumen silk thread includes egg
White silk thread 3 and the functional mass being fixedly connected with 3 surface of albumen silk thread, functional mass are connected with the capture for capturing cell
Mulberry silk, tussah silk or spider silk may be selected in ligand 8, albumen silk thread 3.
9 outer cover of fixing pipe is equipped with guide pin 2, is fixedly connected with sealing-plug 4 in guide pin 2, sealing-plug 4 is surrounded on fixing pipe 9
The outside of upper end, sealing-plug 4, fixing pipe 9 surround the cavity of one end band opening, the other end of functionalization albumen silk thread with guide pin 2
It is within the cavity, and 9 free movement of pipe can be relatively fixed.
Liquid storage unit is removably connected at the syringe needle of guide pin 2, liquid storage unit includes liquid storage set 1, sealing cover 14 and position
The preservation liquid 13 in 1 is covered in liquid storage, through-hole is offered on sealing cover 14, the syringe needle of guide pin 2 is arranged in through-hole and covers 1 with liquid storage
Connection, 1 interior be filled with of interior and above-mentioned cavity of liquid storage set preserve liquid 13.Preservation liquid 13 makes the capture on functionalization albumen silk thread
Ligand 8 will not inactivate during preservation.
The lower end of fixing pipe 9 is fixedly connected with conducting wire pole 11, and conducting wire pole 11 is located in guide pin 2 and can be slided relatively with guide pin 2
It is dynamic.One end of conducting wire pole 11 is located at outside guide pin 2.The inner wall of guide pin 2 is oriented to plug 5, one of ring there are two being also fixedly connected
It is around in the outside of 9 lower end of fixing pipe, another is surrounded on 11 outside of conducting wire pole.
2 external stability of guide pin is connected with the guide pin handle 6 of sheet, the end of conducting wire pole 11 and the seal wire for being connected with sheet
Several anti-skidding salient points 15, the material of guide pin handle 6 and seal wire handle 12 are equipped on handle 12, guide pin handle 6 and seal wire handle 12
Matter is elastic material, convenient to fix when in use on the skin.Aperture is offered on guide pin handle 6 and seal wire handle 12, it is small
Position line 7 is fixedly connected in hole.The setting of position line 7 enables to conducting wire pole 11 in sliding opposite with guide pin 2, one
End will not be skidded off out of guide pin 2.
It is fixed with the functionalization albumen silk thread of capture ligands 8 and its is fixedly connected in fixing pipe 9 (i.e. internal CTC cells
The preparation of capture duct) method it is as follows：
(1) Protein filament taken out (including mulberry silk, tussah silk or spider silk) is put into fixing pipe, injection hydrogel is solid
Determine Protein filament, then Protein filament is put into degumming liquid and carries out degumming.Protein filament after degumming is cleaned repeatedly with tri-distilled water, that is, is obtained
Obtained ankyrin silk.
(2) ankyrin silk (including fixing pipe) is put into round-bottomed bottle, uses diglycidyl ether (including poly- second two respectively
Alcohol diglycidyl ether or 1,4-butanediol diglycidyl ether) and PAA, or diglycidyl ether (including poly- second is used respectively
Hexanediol diglycidyl ether or 1,4- butanediol diglycidyl ethers), polyamine compounds (ethylenediamine, propane diamine, butanediamine or
PEI it) is reacted 6 hours in 60 DEG C of water-bath with PAA.Later, wash with distilled water repeatedly to get surface-functionalized fixation egg
White silk.
(3) surface-functionalized ankyrin silk is put into round-bottomed bottle, EDC and NHS solution, oscillation, at 37 DEG C is added
Water-bath in react 3 hours.After reaction, wash with distilled water repeatedly.It puts it into round-bottomed bottle again, catching for CTC is added
Ligand (epithelial cell adhesion molecule antibody, aptamer or polypeptide aptamer) is obtained, vibrates, is reacted 3 hours in 37 DEG C of water-bath.
After reaction, wash with distilled water repeatedly.It puts it into round-bottomed bottle again, alkanamine is added, oscillation is anti-in 25 DEG C of water-bath
It answers 4 hours.After reaction, wash with distilled water repeatedly to get internal CTC cell captures pipe.
, can be as needed when target acquistion cell is other rare cells, connected by functional mass on albumen silk thread 3
Connect different capture ligands 8.
According to above structure, internal CTC cell captures pipe and other component are assembled into internal CTC cell captures device, filled
With liquid storage set 1 after the completion, is opened, liquid 13 is preserved by being filled in cavity where 2 end of guide pin to functionalization albumen silk thread, and
It is equally added in liquid storage set 1 and preserves liquid 12, sealing liquid storage set 1, sterilizing is spare.
Steps are as follows for the use of the internal CTC cell captures device of the present invention：
In use, guide pin 2 is extracted from liquid storage set 1, about 0.5-1.0cm in blood vessel is then inserted the needle into.Then it pushes away
Dynamic 11 about 2cm of conducting wire pole, conducting wire pole 11 push fixing pipe 9 and its functionalization albumen silk thread of connection with respect to the movement of guide pin 2 and from
Expose in guide pin 2, so that fixing pipe 9 is exposed among blood vessel completely.It is fixed in fixing pipe 9 in blood flow at high speed
A large amount of functionalization albumen silk threads swung in the blood high speed of flowing, fully contacted with CTC, make its immobilized CTC above
Capture ligands and acceptor interaction on CTC, to keep CTC immobilized on albumen silk thread 3.It, can be by seal wire in acquisition procedure
Handle 12 and guide pin handle 6 are fixed on the skin with adhesive tape, do not influence patient activity.After capturing a period of time, conducting wire pole is pulled
11, make it that capture duct be driven to be again introduced into guide pin 2, then pulls seal wire handle 12, guide pin 2 is extracted from blood vessel, that is, is completed
Internal CTC cell captures process.
In order to verify the effect of CTC cell capture devices in vivo of the invention, capture duct is released from guide pin, function therein
It is completely exposed to change albumen silk thread, is cleaned with PBS, scavenging period 2min.The capture duct of cleaning is put into pancreatin and digests 3min,
Culture medium is added and terminates digestion, takes out grabber.Finally, the captured CTC in culture medium is counted and is analyzed.In order to
As a contrast, albumen silk thread of the selection without any processing makes cell capture device, using same method catch in CTC bodies
It obtains.To selectivity that CTC is captured, the results are shown in Figure 3 for two groups of experiments, from figure 3, it can be seen that the CTC cell capture devices of the present invention
Cell capture efficiency is apparently higher than control experiment.Fig. 4-5 is the microphoto of the CTC of two groups of cell capture devices capture, arrow in figure
Pointed head is CTC cells, and the CTC that grabber of the invention is captured is significantly more than control group.
Change fixed functionalization albumen silk thread radical in cell capture device, CTC captures number and also therefore changes, and Fig. 6 is
Functionalization albumen silk thread radical captures number relational graph with CTC, it is seen that with the increasing of functionalization albumen silk thread radical
Add, CTC captures number also increases.This has been also further demonstrated that using the albumen silk thread of more minor diameters as the grabber of vector construction
Capture rate can significantly increase, that is, be apparently higher than the grabber built with single carrier material.
Can also immobilized nanoparticle, Fig. 7 be this hair in functionalization albumen silk thread in addition, in order to change CTC capture rates
The stereoscan photograph of fixed functionalization albumen silk thread in clear-cells grabber；Fig. 8 a are the scanning electricity of immobilized silicon nanoparticle
Mirror photo, Fig. 8 b are the enlarged drawing of silicon nanoparticle in Fig. 8 a.
Fig. 9-10 is Toxic test results and hemolytic experiment test result of the different material to cell, and Fig. 9-10 shows this
The functionalization albumen silk thread of invention is smaller to cytotoxicity, and hemolytic is minimum, almost suitable with negative sample.Silk in figure
Line-ligand is the functionalization albumen silk thread that the present invention uses.Figure 11 is the solidifying of functionalization albumen silk thread and medical stainless steel silk
Courageous and upright experimental test result illustrates that the functionalization albumen silk thread of the present invention is currently being widely used medical stainless steel with what is be commercialized
Silk is hemagglutinin similar, can be applied to human body.
The above is only a preferred embodiment of the present invention, it is not intended to restrict the invention, it is noted that for this skill
For the those of ordinary skill in art field, without departing from the technical principles of the invention, can also make it is several improvement and
Modification, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of using functionalization albumen silk thread as the internal cell capture device of carrier, it is characterised in that：Including with functionalization albumen
Silk thread is the internal cell capture pipe of carrier, and the internal cell capture pipe includes a fixing pipe, several functionalization flexible
Albumen silk thread and the cell capture ligand being fixed on functionalization albumen silk thread, the cell capture ligand are thin for capturing
One end of born of the same parents, the functionalization albumen silk thread are fixed in the fixing pipe, and the other end of the functionalization albumen silk thread can phase
To the fixing pipe freely activity, the outer cover of the fixing pipe is equipped with guide pin, is fixedly connected with sealing-plug in the guide pin, institute
Sealing-plug to be stated to be surrounded on the outside of the fixing pipe, the sealing-plug, fixing pipe surround the cavity of one end band opening with the guide pin,
The other end of the functionalization albumen silk thread is located in the cavity, and the fixing pipe is also associated with conducting wire pole, the conducting wire pole
And it can opposite sliding with the guide pin in the guide pin.
2. internal cell capture device according to claim 1, it is characterised in that：The functionalization albumen silk thread includes albumen
Silk thread and the functional mass being fixedly connected with the albumen wire surface, the functional mass connect the cell capture and match
Body.
3. internal cell capture device according to claim 2, it is characterised in that：The albumen silk thread is mulberry silk, tussah
Silk or spider silk, the functional mass are the compound containing functional group or the compound containing functional group and functionalization
Nanoparticle, the cell capture ligand are one or more of specific antibody, specific nucleic acid aptamer and polypeptide aptamer.
4. internal cell capture device according to claim 3, it is characterised in that：The compound containing functional group be containing
Epoxy compounds, carboxylated compound and one or more of containing amino-compound.
5. internal cell capture device according to claim 3, it is characterised in that：The nanoparticle of the functionalization is titanium dioxide
One kind in silicon nanoparticle and titanium dioxide nano granule.
6. internal cell capture device according to claim 1, it is characterised in that：One end of the functionalization albumen silk thread is solid
Due in the fixing pipe, fixation is fixed by hydrogel.
7. internal cell capture device according to claim 1, it is characterised in that：The cell is circulating tumor cell or mother
Tire source property cell in source property blood.
8. internal cell capture device according to claim 1, it is characterised in that：The guide pin far from the conducting wire pole one
End is removably connected with liquid storage unit, and the liquid storage unit includes liquid storage set, sealing cover and the guarantor in liquid storage set
Liquid storage, through-hole is offered on the sealing cover, and the guide pin is arranged in the through-hole and is connected to liquid storage set, the chamber
It is filled with the preservation liquid in vivo.
9. internal cell capture device according to claim 1, it is characterised in that：The inner wall of the guide pin is also fixedly connected with
Multiple guiding plugs.
10. internal cell capture device according to claim 1, it is characterised in that：The guide pin external stability is connected with piece
The guide pin handle of shape, the one end of the conducting wire pole far from the fixing pipe are connected with the seal wire handle of sheet, the guide pin handle
It is connected with position line between seal wire handle.
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| CN201810321288.0A CN108392232B (en) | 2018-04-11 | 2018-04-11 | In vivo cell capturing device using functional protein silk thread as carrier |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112844342A (en) * | 2021-01-20 | 2021-05-28 | 季华实验室 | Functionalized substrate for enriching circulating tumor cells and preparation method thereof |
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| CN112844342A (en) * | 2021-01-20 | 2021-05-28 | 季华实验室 | Functionalized substrate for enriching circulating tumor cells and preparation method thereof |
| CN112844342B (en) * | 2021-01-20 | 2023-01-31 | 季华实验室 | Functionalized substrate for enriching circulating tumor cells and preparation method thereof |
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