CN106248978B - A kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket - Google Patents
A kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket Download PDFInfo
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- CN106248978B CN106248978B CN201610642966.4A CN201610642966A CN106248978B CN 106248978 B CN106248978 B CN 106248978B CN 201610642966 A CN201610642966 A CN 201610642966A CN 106248978 B CN106248978 B CN 106248978B
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- cup
- miillpore filter
- cup holder
- negative
- bottom plate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
Abstract
The invention discloses a kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket, it includes flexible cup holder to fixing bracket, and flexible cup holder is closely bonded and formed by miillpore filter cup and cup holder bottom plate；The position of corresponding installation miillpore filter cup is provided with cup holder bottom plate mesh on cup holder bottom plate, it is provided with negative-pressure sealing bowl immediately below cup holder bottom plate mesh, negative-pressure sealing bowl is embedded in supporting plate and is locked on cup holder bottom plate by fixing buckle, and negative-pressure sealing bowl lower end is connected with the negative pressure equipment for making its content attract downwards.The object of the present invention is to provide a kind of miillpore filter cup fixation of offer, heating and negative pressure control method and its fixing brackets, facilitate automation quickly fixed miillpore filter cup, realize the automation integrated operation of fixation, dyeing, vacuum cavitations, heating, reduce operational sequence, the operating time is saved, the program that sample shifts when detecting reduces precision higher.
Description
Technical field
The present invention relates to one kind to be tested using membrane separation technique in the fields such as medicine, biology, environmentology, be detected
Automation fixation, heating and negative pressure control method.
Background technology
Tangible substance in being made a collection of specimens using miillpore filter and directly filter membrane progress transparent processing is set under microscope
It is a novel morphologic detection technology to carry out observation, can be widely applied to the small tangible of medical test and other field
Substance detects, but since miillpore filter softness and rapid wear lead to having one side to be limited to, the method used is substantially
It is affixed directly to Double-layered cup bottom or is mechanically fixed by Double-layered cup.
Patent one：Dyeing, the flaking method of Chinese Patent Application No. 201010529398.X, a kind of cell or bacterium,
Dyeing, the flaking method for disclosing a kind of cell or bacterium in medical test and biological study, using filter membrane as sample
Piece substrate dyed, film-making, using filtration type Double-layered cup as dyeing, film-making vessel, the filtration type Double-layered cup, cup body
Peripheral wall closing, be only equipped with hole in top and bottom, upper and lower two-part filtering can will be divided by being equipped in cup body inside cup body
Film, the filter membrane that the liquid such as staining reagent are not equipped in container under normal pressure penetrate, and pass through the pressure being arranged on filter membrane both sides
Difference retains bacterium or cell, and the liquid other than cell or bacterium is contaminated through filter membrane through the mode excluded
Color film-making, the present invention can be automatically performed using equipment cell and nacterial smear, dyeing, film-making and use simply, cost of use it is low.
Its flaking method and photo making cup for announcing, difficulty is larger when using automation equipment operation.
Patent two：Chinese patent ZL201320852375.1 discloses a kind of negative pressure automatic smear, overflow dyeing machine, in body
On be provided with the centrifuge of centrifugal turntable, waste collecting device is equipped on the body below centrifugal turntable, in centrifugal turntable
24 cup holders are equipped with, perforated filter film Double-layered cup is placed in cup holder, 24 liquid feedings are equipped on the body above cup holder
Head, the liquid feeding head are connect with reagent container by reagent pipeline, are equipped with constant displacement pump on reagent pipeline, are also installed on body
There is control centrifugal turntable centrifugal rotation and positions and control the computer that constant displacement pump starts or stops work and amount of reagent, this reality
With New Energy be automatically performed to bacterium, cell smear, dyeing, can complete different colouring methods, coloring it is reproducible,
Recall rate is high, efficient, safe operation is simple, manufacturing cost and cost of use are low.
It is not related to filtered heating operation when carrying out Senile Mouse using miillpore filter（Sample needs
It is detected after drying）, additional equipment and operation sequence are so needed, causes to detect relative complex cumbersome；Its correspondence simultaneously
Test cup fixed frame is directed to a kind of cup body structure of existing patent, and in actual mechanical process, micropore filtering film picks and places
It is comparatively laborious, and more demanding, unsuitable full-automatic operation is operated, reduce the efficiency and precision of detection.
Invention content
The purpose of the present invention is in view of the above problems, providing a kind of miillpore filter cup fixation, heating and negative pressure control method
And its fixing bracket, facilitate automation quickly fixed miillpore filter cup, realizes the automation of fixation, dyeing, vacuum cavitations, heating
One operation, reduces operational sequence, saves the operating time, and the program that sample shifts when detecting reduces precision higher.
In order to achieve the above object, the technical solution adopted by the present invention is：
A kind of miillpore filter cup fixing bracket comprising flexible cup holder（3）, flexible cup holder（3）By being used to fix micropore filter
Film cup（4）Flexible material and its bottom part down setting cup holder bottom plate（2）Closely bond composition；In cup holder bottom plate（2）Shang pair
Miillpore filter cup should be installed（4）Position be provided with cup holder bottom plate mesh（7）, in cup holder bottom plate mesh（7）Underface setting
There is negative-pressure sealing bowl（8）, the negative-pressure sealing bowl（8）It is embedded in supporting plate（1）It is interior and pass through fixing buckle（10）It is locked at cup holder bottom
Plate（2）On, the negative-pressure sealing bowl（8）Lower end is connected with the negative pressure equipment for making its content attract downwards.
Further, the supporting plate（1）Heating device is inside installed（9）.
Further, the negative-pressure sealing bowl（8）Top be provided with obturating cup seal（6）, it is higher than supporting plate（1）And
It is centered around cup holder bottom plate mesh（7）Surrounding formed sealing structure.
Further, the flexible cup holder（3）On be provided with several clear openings, and with miillpore filter cup to be fixed（4）
It corresponds.
The present invention discloses use above-mentioned support bracket fastened control method：
A kind of miillpore filter cup fixation, heating and negative pressure control method comprising following several parts：
1）, pass through flexible cup holder（3）, supporting plate（1）, negative-pressure sealing bowl（8）, fixing buckle（10）With the use of composition, micropore
Filter membrane cup（4）It is packed into flexible carrier（3）It is internal；And its bottom miillpore filter（5）It is close to cup holder bottom plate（2）In cup holder bottom plate mesh
（7）；
2）, by miillpore filter cup（4）Bottom periphery and flexible cup holder（3）Form sealing, negative-pressure sealing bowl（8）It is close to cup holder
Bottom plate（2）No mesh portion and it is centered around cup holder bottom plate mesh（7）Surrounding formed sealing structure；
3）, by negative-pressure sealing bowl（8）Lower end connection makes the negative pressure equipment that its content attracts downwards, negative pressure pass through cup holder bottom
Expanded metals hole（7）Pass through miillpore filter for liquid（5）Filtering provides power；
4）, by supporting plate（1）Interior installation heating device（9）, it is miillpore filter（5）Heat source is provided；
5）, flexible cup holder（3）Pass through fixing buckle（10）With supporting plate（1）It combines closely.
Further, the flexible cup holder（3）By being provided with the flexible material of several clear openings and opening meshed cup holder bottom
Plate（2）Composition is closely bonded, miillpore filter cup is placed（4）Hole be aligned cup holder bottom plate（2）On mesh portion, cup holder bottom plate
（2）It can be quickly by supporting plate（1）The even heat of generation passes to miillpore filter（5）, make miillpore filter cup（4）It can set
Determine to be operated at temperature.
Further, supporting plate（1）On negative-pressure sealing bowl is installed（8）, heating device（9）, fixing buckle（10）, negative-pressure sealing
Bowl（8）Obturating cup seal（6）Higher than supporting plate（1）Plane 1-5mm, in fastening fixing buckle（10）When with cup holder bottom plate（2）Nothing
Mesh portion, which forms to be fitted close, generates sealing effect.
Further, flexible cup holder（3）Being can be by unclamping fixing buckle（10）With supporting plate（1）The separate unit of separation,
For loading miillpore filter cup in batches（4）, each flexible cup holder（3）Multiple hole positions can be arranged above, load multiple micropores
Filter membrane cup（4）；Supporting plate（1）On negative-pressure sealing bowl（8）It is corresponded with cup holder hole position.
Further, heating device（9）Temperature guarantee, heating device are provided according to requirement of experiment（9）Including heater element
And temperature control component.
Further, negative-pressure sealing bowl（8）Stable negative pressure is obtained by external vacuum pump and negative pressure control apparatus.
Beneficial effects of the present invention：The present invention provides a kind of miillpore filter cup fixation, heating and negative pressure control method and
Its fixing bracket facilitates automation quickly fixed miillpore filter cup, realizes the automation one of fixation, dyeing, vacuum cavitations, heating
Gymnastics is made, and reduces operational sequence, saves the operating time, and the program that sample shifts when detecting reduces precision higher.
1, fast and easy carries out picking and placeing miillpore filter cup.
2, it during observation, without detaching miillpore filter again, avoids miillpore filter broken, facilitates microscopic examination, letter
Change operation sequence, saves the operating time.
Description of the drawings
Fig. 1 is the fixed heated holder structural schematic diagram of the present invention.
Fig. 2 is miillpore filter cup and miillpore filter structural schematic diagram in Fig. 1.
Label character described in figure is expressed as：1, supporting plate；2, cup holder bottom plate；3, flexible cup holder；4, miillpore filter cup；5, micro-
Hole filter membrane；6, obturating cup seal；7, cup holder bottom plate mesh；8, negative-pressure sealing bowl；9, heating device；10, fixing buckle；41, cup
Body annular groove；42, ring plate mouth is taken；5, miillpore filter；51, film ring is filled.
Specific implementation mode
In order to make those skilled in the art more fully understand technical scheme of the present invention, below in conjunction with the accompanying drawings to the present invention into
The description of row detailed description, this part is only exemplary and explanatory, should not there is any limitation to protection scope of the present invention
Effect.
As shown in Figure 1, a kind of technical solution of the present invention：A kind of miillpore filter cup fixing bracket comprising flexible cup holder 3,
Flexible cup holder 3 closely bonds group by the cup holder bottom plate 2 of flexible material and the setting of its bottom part down for fixing miillpore filter cup 4
At；The position of corresponding installation miillpore filter cup 4 is provided with cup holder bottom plate mesh 7 on cup holder bottom plate 2, in cup holder bottom plate mesh 7
Underface be provided with negative-pressure sealing bowl 8, the negative-pressure sealing bowl 8 is embedded in supporting plate 1 and is locked at cup by fixing buckle 10
On frame bottom plate 2,8 lower end of negative-pressure sealing bowl is connected with the negative pressure equipment for making its content attract downwards.
Preferably, heating device 9 is installed in the supporting plate 1.
Preferably, the top of the negative-pressure sealing bowl 8 is provided with obturating cup seal 6, higher than supporting plate 1 and is centered around cup
The surrounding of frame bottom plate mesh 7 forms sealing structure.
Preferably, several clear openings are provided on the flexible cup holder 3, and a pair of with miillpore filter cup 4 one to be fixed
It answers.
The miillpore filter cup 4 being used in combination with the present invention is disclosed in Fig. 2, bottom of cup end face is provided with cup body card
Annular groove 41, matched 5 upper surface of miillpore filter is fixed with dress film ring 51, and it is embedded into cup body annular groove 41, in cup
Body bottom outside be arranged it is useful remove dress film ring 51 take ring plate mouth 42.
The present invention discloses use above-mentioned support bracket fastened control method：
A kind of miillpore filter cup fixation, heating and negative pressure control method comprising following several parts：
1）, by flexible cup holder 3, supporting plate 1, negative-pressure sealing bowl 8, fixing buckle 10 with the use of composition, miillpore filter cup 4 fill
Enter inside flexible carrier 3；And its bottom miillpore filter 5 is close to the cup holder bottom plate mesh 7 in cup holder bottom plate 2；
2）, 4 bottom periphery of miillpore filter cup formed with flexible cup holder 3 seal, negative-pressure sealing bowl 8 is close to cup holder bottom plate 2
No mesh portion and the surrounding formation sealing structure for being centered around cup holder bottom plate mesh 7；
3）, so that the negative pressure equipment that its content attracts downwards, negative pressure is passed through cup holder bottom plate the connection of 8 lower end of negative-pressure sealing bowl
Mesh 7 provides power for liquid by the filtering of miillpore filter 5；
4）, heating device 9 will be installed in supporting plate 1, provide heat source for miillpore filter 5；
5）, flexible cup holder 3 combined closely by fixing buckle 10 and supporting plate 1.
Preferably, the flexible cup holder 3 is tight by being provided with the flexible material of several clear openings and opening meshed cup holder bottom plate 2
Close bonding composition, places the mesh portion on the hole alignment cup holder bottom plate 2 of miillpore filter cup 4, cup holder bottom plate 2 can quickly by
The even heat that supporting plate 1 generates passes to miillpore filter 5, and miillpore filter cup 4 is allow to be operated at a set temperature.
Preferably, negative-pressure sealing bowl 8, heating device 9, fixing buckle 10, the sealing of negative-pressure sealing bowl 8 are installed on supporting plate 1
Bowl seal 6 is higher than 1 plane 1-5mm of supporting plate, is formed and is fitted close without mesh portion with cup holder bottom plate 2 when fastening fixing buckle 10
Generate sealing effect.
Preferably, flexible cup holder 3 is the separate unit that can be detached with supporting plate 1 by unclamping fixing buckle 10, for batch
Miillpore filter cup 4 is loaded, each flexible cup holder 3 can arrange multiple hole positions above, load multiple miillpore filter cups 4；Supporting plate 1
On negative-pressure sealing bowl 8 and cup holder hole position correspond.
Preferably, heating device 9 provides temperature guarantee according to requirement of experiment, and heating device 9 includes heater element and temperature
Control element.
Preferably, negative-pressure sealing bowl 8 obtains stable negative pressure by external vacuum pump and negative pressure control apparatus.
It should be noted that herein, the terms "include", "comprise" or its any other variant are intended to non-row
His property includes, so that the process, method, article or equipment including a series of elements includes not only those elements, and
And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic
Element.
Principle and implementation of the present invention are described for specific case used herein, the explanation of above example
It is merely used to help understand the method and its core concept of the present invention.The above is only a preferred embodiment of the present invention, it answers
When pointing out due to the finiteness of literal expression, and objectively there is unlimited concrete structure, for the common skill of the art
For art personnel, without departing from the principle of the present invention, several improvement, retouching or variation can also be made, can also incited somebody to action
Above-mentioned technical characteristic is combined in the right way；These improve retouching, variation or combination, or the not improved structure by invention
Think and technical solution directly applies to other occasions, is regarded as protection scope of the present invention.
Claims (6)
1. a kind of miillpore filter cup fixation, heating and negative pressure control method, which is characterized in that it includes that miillpore filter cup fixes branch
Frame, miillpore filter cup fixing bracket include flexible cup holder（3）, flexible cup holder（3）By being used to fix miillpore filter cup（4）Flexibility
The cup holder bottom plate of material and the setting of its bottom part down（2）Closely bond composition；In cup holder bottom plate（2）Upper corresponding installation miillpore filter
Cup（4）Position be provided with cup holder bottom plate mesh（7）, in cup holder bottom plate mesh（7）Underface be provided with negative-pressure sealing bowl
（8）, the negative-pressure sealing bowl（8）It is embedded in supporting plate（1）It is interior and pass through fixing buckle（10）It is locked at cup holder bottom plate（2）On, it is described
Negative-pressure sealing bowl（8）Lower end is connected with the negative pressure equipment for making its content attract downwards；The supporting plate（1）Heating dress is inside installed
It sets（9）；The negative-pressure sealing bowl（8）Top be provided with obturating cup seal（6）, it is higher than supporting plate（1）And it is centered around cup holder
Bottom plate mesh（7）Surrounding formed sealing structure；The miillpore filter cup（4）Bottom end face is provided with cup body annular groove（41）,
Matched miillpore filter（5）Upper surface is fixed with dress film ring（51）, and it is embedded into cup body annular groove（41）It is interior, in cup body
Bottom outside setting is useful to remove dress film ring（51）Take ring plate mouth（42）；
The fixation of miillpore filter cup, heating and negative pressure control method include following several parts：
1）, pass through flexible cup holder（3）, supporting plate（1）, negative-pressure sealing bowl（8）, fixing buckle（10）With the use of composition, miillpore filter
Cup（4）It is packed into flexible cup holder（3）It is internal；And its bottom miillpore filter（5）It is close to cup holder bottom plate（2）In cup holder bottom plate mesh
（7）；
2）, by miillpore filter cup（4）Bottom periphery and flexible cup holder（3）Form sealing, negative-pressure sealing bowl（8）It is close to cup holder bottom plate
（2）No mesh portion and it is centered around cup holder bottom plate mesh（7）Surrounding formed sealing structure；
3）, by negative-pressure sealing bowl（8）Lower end connection makes the negative pressure equipment that its content attracts downwards, negative pressure pass through cup holder bottom plate net
Hole（7）Pass through miillpore filter for liquid（5）Filtering provides power；
4）, by supporting plate（1）Interior installation heating device（9）, it is miillpore filter（5）Heat source is provided；
5）, flexible cup holder（3）Pass through fixing buckle（10）With supporting plate（1）It combines closely；
The flexibility cup holder（3）By being provided with the flexible material of several clear openings and opening meshed cup holder bottom plate（2）It closely bonds
Composition places miillpore filter cup（4）Hole be aligned cup holder bottom plate（2）On mesh portion, cup holder bottom plate（2）Can quickly by
Supporting plate（1）The even heat of generation passes to miillpore filter（5）, make miillpore filter cup（4）It can be grasped at a set temperature
Make.
2. a kind of miillpore filter cup fixation, heating and negative pressure control method according to claim 1, which is characterized in that institute
State flexible cup holder（3）On be provided with several clear openings, and with miillpore filter cup to be fixed（4）It corresponds.
3. a kind of miillpore filter cup fixation, heating and negative pressure control method according to claim 1, which is characterized in that support
Plate（1）On negative-pressure sealing bowl is installed（8）, heating device（9）, fixing buckle（10）, negative-pressure sealing bowl（8）Obturating cup seal
（6）Higher than supporting plate（1）Plane 1-5mm, in fastening fixing buckle（10）When with cup holder bottom plate（2）No mesh portion, which is formed, to be fitted close
Generate sealing effect.
4. a kind of miillpore filter cup fixation, heating and negative pressure control method according to claim 1, which is characterized in that soft
Property cup holder（3）Being can be by unclamping fixing buckle（10）With supporting plate（1）The separate unit of separation, for loading miillpore filter in batches
Cup（4）, each flexible cup holder（3）Multiple hole positions can be arranged above, load multiple miillpore filter cups（4）；Supporting plate（1）On
Negative-pressure sealing bowl（8）It is corresponded with cup holder hole position.
5. a kind of miillpore filter cup fixation, heating and negative pressure control method according to claim 1, which is characterized in that add
Thermal（9）Temperature guarantee, heating device are provided according to requirement of experiment（9）Including heater element and temperature control component.
6. a kind of miillpore filter cup fixation, heating and negative pressure control method according to claim 1, which is characterized in that negative
Means of press seals bowl（8）It is obtained by external vacuum pump and negative pressure control apparatus and stablizes negative pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610642966.4A CN106248978B (en) | 2016-08-08 | 2016-08-08 | A kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610642966.4A CN106248978B (en) | 2016-08-08 | 2016-08-08 | A kind of miillpore filter cup fixation, heating and negative pressure control method and its fixing bracket |
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| Publication Number | Publication Date |
|---|---|
| CN106248978A CN106248978A (en) | 2016-12-21 |
| CN106248978B true CN106248978B (en) | 2018-07-20 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108693008B (en) * | 2018-07-25 | 2024-01-30 | 湖南省天骑医学新技术股份有限公司 | A millipore filter cup collection device for millipore filter shifts |
| CN115406188B (en) * | 2022-08-16 | 2023-12-15 | 湖南省天骑医学新技术股份有限公司 | Filter membrane rapid drying treatment method and system |
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| US6225130B1 (en) * | 1998-02-19 | 2001-05-01 | Fuji Photo Film Co., Ltd. | Method of separating serum from whole blood |
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
| CN203732340U (en) * | 2013-12-23 | 2014-07-23 | 湖南省天骑医学新技术有限公司 | Automatic negative pressure staining and dyeing machine |
| CN203894092U (en) * | 2013-12-23 | 2014-10-22 | 湖南省天骑医学新技术有限公司 | Filtering membrane interlayer cup |
| CN205982295U (en) * | 2016-08-08 | 2017-02-22 | 湖南省天骑医学新技术股份有限公司 | Millipore filtration cup fixed bolster |
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2016
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6225130B1 (en) * | 1998-02-19 | 2001-05-01 | Fuji Photo Film Co., Ltd. | Method of separating serum from whole blood |
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
| CN203732340U (en) * | 2013-12-23 | 2014-07-23 | 湖南省天骑医学新技术有限公司 | Automatic negative pressure staining and dyeing machine |
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| CN106248978A (en) | 2016-12-21 |
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