CN211227137U - Sample treatment and filtration device for detecting microorganisms in clinical samples - Google Patents
Sample treatment and filtration device for detecting microorganisms in clinical samples Download PDFInfo
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- CN211227137U CN211227137U CN201921686891.5U CN201921686891U CN211227137U CN 211227137 U CN211227137 U CN 211227137U CN 201921686891 U CN201921686891 U CN 201921686891U CN 211227137 U CN211227137 U CN 211227137U
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- 244000005700 microbiome Species 0.000 title claims abstract description 42
- 238000001914 filtration Methods 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 60
- 239000012528 membrane Substances 0.000 claims abstract description 30
- 238000012545 processing Methods 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 13
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 5
- 230000002452 interceptive effect Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004856 Lectins Human genes 0.000 description 2
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- 210000004027 cell Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 101710186708 Agglutinin Proteins 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
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Abstract
The utility model discloses a sample processing and filtering device for detecting microorganisms in clinical samples, which comprises a first filter cup, a centrifuge tube which can be combined with the first filter cup, and a flat bottom detection bottle for detection and observation; the first filter cup is combined on the upper part of the centrifugal tube, and the bottom of the first filter cup is provided with a first filter membrane with the specification smaller than that of target microorganisms in the sample; the flat-bottom detection bottle is provided with a bottle cap capable of tightly covering the bottle, and a supporting platform is arranged at the upper part in the bottle and used for placing a first filter cup. The upper part of the first filter cup can be also provided with a detachable second filter cup, and the bottom of the second filter cup is provided with a second filter membrane with the specification larger than that of the target microorganism in the sample and smaller than that of the interferents in the sample. The sample is processed by using the utility model, the process is simple, and the operation is very quick and effective; the obtained sample can be directly used for the detection step, so that secondary pollution is avoided; the device is simple, the cost is low, and the cost is greatly saved; and the detection rate can be improved, and the method has good application value.
Description
Technical Field
The utility model relates to a sample processing and filtering device for detecting microorganisms in clinical samples.
Background
In detecting the presence of a microorganism or a target substance in a clinical sample, it is often necessary to process the sample to remove interfering substances and cells, or to enrich the target microorganism or other target substance for detection. Especially for high sensitivity detection, sample preparation is a crucial step, because the concentration of target microorganisms and interfering substances in the sample may cause a great influence on the detection result. In general, for the removal of interference, an immunomagnetic microparticle method is generally used to specifically remove interfering substances such as interfering cells in a sample by species-specific antibodies or lectins/saccharides, and then to perform enrichment and detection. Numerous experiments have demonstrated that this method is fast and efficient, however, the application of this method is premised on the need to develop corresponding species-specific antibodies or lectins/carbohydrates, which is rather laborious and cumbersome. There is therefore a need for an equally effective, but simpler and faster method to achieve the removal of interfering factors from clinical samples and/or the enrichment of target microorganisms for detection.
SUMMERY OF THE UTILITY MODEL
To the problem that interference material exists in the present removal sample, the utility model provides a filter equipment that is arranged in microorganism detection sample to handle among clinical sample effectively gets rid of the interference material in the sample through adopting the filtration (including practical centrifuge and vacuum filtration) and/or enrichment target microorganism to better detect. In order to achieve the purpose, the specific technical scheme is as follows:
a sample processing and filtering device for detecting microorganisms in clinical samples comprises a first filter cup, a centrifuge tube which can be combined with the first filter cup, and a flat-bottom detection bottle for detection and observation; the first filter cup is combined on the upper part of the centrifugal tube, and the bottom of the first filter cup is provided with a first filter membrane with the specification smaller than that of target microorganisms in the sample; the flat-bottom detection bottle is provided with a bottle cap capable of tightly covering the bottle, and a supporting platform is arranged at the upper part in the bottle and used for placing a first filter cup.
In the sample processing and filtering device for detecting microorganisms in clinical samples, the upper part of the first filter cup is further provided with a detachable second filter cup, and the bottom of the second filter cup is provided with a second filter membrane which has a size larger than that of target microorganisms in the sample and smaller than that of interferents in the sample.
Preferably, in the filter device for processing the microbial detection sample in the clinical sample, the bottle cap of the flat-bottom detection bottle is provided with an air-permeable membrane hole; further preferably, the bottle cap of the flat-bottom detection bottle can be a rubber plug.
Preferably, in the filter device for processing a microorganism detection sample in a clinical sample, the first filter cup and the second filter cup are both made of EP materials; the flat bottom detection bottle is a transparent or brown glass bottle.
Preferably, the first filter membrane and the second filter membrane are filters with different specifications. Further preferably, the first filter membrane and the second filter membrane are detachably mounted in the filter cup.
Preferably, in the filter device for processing a microbiological test sample in a clinical sample, the first filter cup rests on the support platform of the flat-bottom test bottle through a filter cup holder, the filter cup holder can be taken out of the flat-bottom test bottle, and is provided with a filter cup hole for inserting the first filter cup.
Has the advantages that:
firstly, the utility model is used for processing the microorganism detection sample in the clinical sample, the whole process can be completed within 1 minute, the operation is very quick and effective, and the effect is obvious; secondly, use the utility model discloses the sample that the filtration obtained can directly be used for the testing procedure, and need not shift the target microorganism, avoids secondary pollution, practicality very. Furthermore, the utility model discloses the device is simple, and the cost is very cheap to save and adopt the immune magnetic particle method, prepare the trouble of species-specific antibody or agglutinin/saccharide, provide new thinking and method for clinical sample handles, and practice thrift the cost. In addition, the filter membrane in the filter cup can be detached and replaced, and filters with different specifications can be selected according to requirements; the filter cup support in the flat-bottom detection bottle can be taken out, the use is simple, the filter cup support can be provided with a plurality of filter cup holes, a plurality of samples can be detected simultaneously, the detection rate is improved, and the application value is good.
Drawings
FIG. 1 is a schematic diagram of a sample processing and filtering device for detecting microorganisms in clinical samples according to the present invention;
FIG. 2 is a schematic view of the structure of the flat bottom detection bottle of the present invention;
fig. 3 is a schematic view of the structure of the filter cup holder of the present invention.
In the figure: 1. a first filter cup; 101. a first filter membrane; 2. a second filter cup; 201. a second filter membrane; 3. centrifuging the tube; 4. a flat bottom detection bottle; 401. a bottle cap; 4011. a gas permeable membrane pore; 402. a support platform; 5. a filter bowl holder; 501. and (4) filtering cup holes.
Detailed Description
The technical solution of the present invention will be described clearly and completely with reference to the following embodiments and the accompanying drawings, and it is to be understood that the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
The present embodiment is a sample processing and filtering apparatus for detecting microorganisms in clinical samples, as shown in fig. 1 to 3, comprising a first filter cup 1, a centrifuge tube 3 capable of combining with the first filter cup 1, and a flat bottom detection bottle 4 for detection and observation; the first filter cup 1 is combined on the upper part of the centrifuge tube 3, and the bottom of the first filter cup 1 is provided with a first filter membrane 101 with the specification smaller than that of target microorganisms in a sample; under the condition that interference does not need to be removed, a sample can be directly added into the first filter cup 1, under the condition of a centrifugal machine or vacuum centrifugation, target microorganisms in the sample are enriched on the first filter membrane 101, then the first filter cup 1 containing the target microorganisms is placed into the flat-bottom detection bottle 4, a supporting platform 402 for placing the first filter cup 1 is arranged at the upper part in the flat-bottom detection bottle 4, a detection reagent is added, a bottle cover 401 is tightly covered, and detection is directly carried out after incubation culture, the whole process can be completed within 1 minute, and the method is very quick and effective; and the filtered sample is directly used for the detection step without transferring the target microorganism, so that secondary pollution is avoided, and the method is very practical.
When the interferent in the clinical sample needs to be removed, the upper part of the first filter cup 1 is also provided with a detachable second filter cup 2, the bottom of the second filter cup 2 is provided with a second filter membrane 201 with the specification larger than that of the target microorganism in the sample but smaller than that of the interferent in the sample, the sample is added into the second filter cup 2, and the purposes of removing the interferent and enriching the target microorganism are achieved simultaneously through one-time centrifugation.
According to the filtering device for processing the microorganism detection sample in the clinical sample, the bottle cap 401 of the flat-bottom detection bottle 4 is provided with the air permeable membrane hole 4011, so that the survival of target microorganisms is facilitated, and the incubation culture with a detection reagent is facilitated; generally, the bottle cap 401 of the flat-bottom detection bottle 4 can be made of a rubber plug directly or made of a rubber plug material.
In the filter device for processing a microorganism detection sample in a clinical sample, the first filter cup 1 and the second filter cup 2 are both made of EP material, and the flat bottom detection bottle 4 is a transparent or brown glass bottle; however, other safe materials for centrifugation and filtration in biological experiments may also be used.
In the filter device for processing a sample for detecting microorganisms in a clinical sample according to the present embodiment, the first filter membrane and the second filter membrane 201 may be filters with different specifications. And the first filter membrane 101 and the second filter membrane 201 are detachably arranged in the filter cup, and a filter with a proper specification can be selected for use according to the requirement in the application of the reagent.
In this embodiment, the first filter cup 1 is placed on the support platform 402 of the flat bottom detection bottle 4 through the filter cup holder 5, the filter cup holder 5 can be taken out from the flat bottom detection bottle 4, a filter cup hole 501 for inserting the first filter cup 1 is formed in the filter cup holder 5, the number of the filter cup holes 501 can be multiple, multiple samples can be detected simultaneously, and the detection efficiency is improved.
Example 2
In this example, the presence and quantity of bacteria in clinical samples were determined using the filter device described in example 1 using an ATP-based detection method.
Respectively selecting a filter with the specification of 0.2 mu m as a filter membrane of the first filter cup 1 for bacteria enrichment; a filter with a size of 5 μm was selected as the filter membrane of the second filter cup 2 for removing somatic cells from the sample. Then, the second filter cup 2 is arranged at the upper part of the first filter cup 1, the combination of the first filter cup and the second filter cup is inserted into a centrifuge tube 3, and 1ml of clinical samples are added into the second filter cup 2; then placing the sample into a centrifuge, centrifuging the sample at the temperature of 4 ℃ for 2min at the speed of 800-1000 rpm, then abandoning the centrifuge tube 3 and the second filter cup 2, inserting the first filter cup 1 into a filter cup hole 501 of a filter cup bracket 5, then placing the sample into a flat-bottom detection bottle 4, adding a reagent containing a fluorescein/luciferase system, incubating the sample for 5 min at room temperature, then directly placing the flat-bottom detection bottle 4 under a photometer to respectively read the light value of ATP, and recording the light Reading (RLU), thereby determining the amount of the ATP in the sample; and determining the presence and quantity of bacteria in the sample by the amount of ATP. For this example, when measured using a photometer, a strong light signal from the sample indicates the presence of the target bacteria in the sample, and the more target bacteria in the sample, the higher the light signal.
It should be understood that although the present description refers to embodiments, not every embodiment contains only a single technical solution, and such description is for clarity only, and those skilled in the art should take the description as a whole, and the technical solutions in the embodiments may be appropriately combined to form other embodiments understood by those skilled in the art.
Claims (9)
1. A sample processing filtration device for the detection of microorganisms in clinical samples, characterized by: comprises a first filter cup (1), a centrifuge tube (3) which can be combined with the first filter cup (1), and a flat-bottom detection bottle (4) for detection and observation; the first filter cup (1) is combined on the upper part of the centrifuge tube (3), and the bottom of the first filter cup (1) is provided with a first filter membrane (101) with the specification smaller than that of target microorganisms in a sample; the flat-bottom detection bottle (4) is provided with a bottle cap (401) capable of tightly covering the bottle, and a supporting platform (402) is arranged at the upper part in the bottle and used for placing the first filter cup (1).
2. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1, characterized in that: the upper part of the first filter cup (1) is also provided with a detachable second filter cup (2), and the bottom of the second filter cup (2) is provided with a second filter membrane (201) with the specification larger than that of the target microorganism in the sample but smaller than that of the interferent in the sample.
3. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1, characterized in that: the bottle cap (401) of the flat-bottom detection bottle (4) is provided with an air permeable membrane hole (4011).
4. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1, characterized in that: the bottle cap (401) of the flat-bottom detection bottle (4) is a rubber plug.
5. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1 or 2, characterized in that: the first filter cup (1) and the second filter cup (2) are both made of EP materials.
6. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1, characterized in that: the flat bottom detection bottle (4) is a transparent or brown glass bottle.
7. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1 or 2, characterized in that: the first filter membrane (101) and the second filter membrane (201) are filters with different specifications.
8. The sample processing filtration device for the detection of microorganisms in clinical samples according to claim 7, characterized in that: the first filter membrane (101) and the second filter membrane (201) are detachably arranged in the filter cup.
9. A sample processing filtration device for the detection of microorganisms in clinical samples according to claim 1, characterized in that: the first filter cup (1) is placed on a supporting platform (402) of the flat-bottom detection bottle (4) through a filter cup support (5), the filter cup support (5) can be taken out of the flat-bottom detection bottle (4), and a filter cup hole (501) for the first filter cup (1) to be inserted is formed in the filter cup support.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921686891.5U CN211227137U (en) | 2019-10-10 | 2019-10-10 | Sample treatment and filtration device for detecting microorganisms in clinical samples |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921686891.5U CN211227137U (en) | 2019-10-10 | 2019-10-10 | Sample treatment and filtration device for detecting microorganisms in clinical samples |
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| CN211227137U true CN211227137U (en) | 2020-08-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113501585A (en) * | 2021-06-29 | 2021-10-15 | 浙江正合谷生物科技有限公司 | Mute centrifugal membrane filter |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113501585A (en) * | 2021-06-29 | 2021-10-15 | 浙江正合谷生物科技有限公司 | Mute centrifugal membrane filter |
| CN113501585B (en) * | 2021-06-29 | 2022-12-13 | 浙江正合谷生物科技有限公司 | Mute centrifugal membrane filter |
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| TR01 | Transfer of patent right |
Effective date of registration: 20220311 Address after: 350000, floor 7, building 16, phase II, innovation park, 7 middle wulongjiang Avenue, high tech Zone, Fuzhou, Fujian Patentee after: RGI (FUZHOU) GENETIC MEDICINE LABORATORY Co.,Ltd. Address before: 215000 No.8 Jinfeng Road, high tech Zone, Suzhou City, Jiangsu Province Patentee before: CELLEX BIOLOGICAL TECHNOLOGY (SUZHOU) CO.,LTD. |
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Address after: 350000, floor 7, building 16, phase II, innovation park, 7 middle wulongjiang Avenue, high tech Zone, Fuzhou, Fujian Patentee after: Fujian Aji'an Biotechnology Co.,Ltd. Country or region after: China Address before: 350000, floor 7, building 16, phase II, innovation park, 7 middle wulongjiang Avenue, high tech Zone, Fuzhou, Fujian Patentee before: RGI (FUZHOU) GENETIC MEDICINE LABORATORY Co.,Ltd. Country or region before: China |