CN108956218B - Slice making clamp for centrifugal liquid-based thin-layer cell slice making machine - Google Patents
Slice making clamp for centrifugal liquid-based thin-layer cell slice making machine Download PDFInfo
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- CN108956218B CN108956218B CN201710370193.3A CN201710370193A CN108956218B CN 108956218 B CN108956218 B CN 108956218B CN 201710370193 A CN201710370193 A CN 201710370193A CN 108956218 B CN108956218 B CN 108956218B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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Abstract
The invention discloses a slide making clamp for a centrifugal liquid-based thin-layer cell slide making machine, which has the technical scheme that the slide making clamp comprises a box body for clamping a glass slide, a liquid storage hopper for storing sample liquid and a flow guide pipe, wherein the box body comprises a front side plate, a rear side plate and a bottom plate for connecting the front side plate and the rear side plate, a painting hole is formed in the position, corresponding to the glass slide, on the front side plate, a painting hole is formed, one end of the flow guide pipe is communicated with the painting hole, the other end of the flow guide pipe is communicated with the liquid storage hopper, and a flow guide part which inclines downwards from the painting hole to the. The invention solves the problems that in the use process of the existing slide clamp, too much cell thin layer sample liquid is accumulated at the opening during centrifugation because the slide glass is propped against the opening, and finally the sample liquid is diffused along the slide glass, so that the background of the slide glass is fuzzy and not clean, and the analysis and the diagnosis are influenced.
Description
Technical Field
The invention relates to liquid-based thin-layer cell detection, in particular to a sheet making clamp for a centrifugal liquid-based thin-layer cell sheet making machine.
Background
The liquid-based thin-layer cell detection method comprises the following steps: sampling by using a sampling brush, rinsing the sampling brush by using a preserving fluid and preserving the sample, shaking and dissolving, flaking liquid-based thin-layer cells and observing by using a microscope. In the prior art, smear preparation is usually carried out manually, when a specimen is prepared manually, exfoliated cells on a glass slide are difficult to coat uniformly, and in addition, due to the fact that the specimen for sampling the exfoliated cells contains mucus, red blood cells and other impurities, the background of a specimen smear is not clean and clear, so that the observation analysis and diagnosis of a pathologist are influenced.
The chinese utility model patent with the publication number of grant CN202393969U discloses a liquid-based thin-layer cell slide making clamp for solving the above-mentioned problems, and this slide making clamp is used and is compared conveniently, but when this slide making clamp was used, because of the slide glass is supported at the opening part, can appear too much cell thin-layer sample liquid during centrifugation and pile up at the opening part, finally make sample liquid along with the slide glass diffusion, lead to the slide glass background fuzzy unclean, influence analysis and diagnosis.
Disclosure of Invention
The invention aims to provide a slide making clamp for a centrifugal liquid-based thin-layer cell slide maker, which has the characteristic that the background of a prepared cell slide is cleaner.
The above object of the present invention is achieved by the following technical solutions: the utility model provides a centrifugal liquid-based thin-layer cell pelleter is with system piece clamp, includes the box body that is used for the clamping slide glass, is used for storing the stock solution fill and the honeycomb duct of appearance liquid, and the box body includes preceding curb plate, posterior lateral plate and is used for connecting the bottom plate of preceding curb plate and posterior lateral plate, the hole of dying has been seted up to scribbling in the position department that corresponds the slide glass on the preceding curb plate, honeycomb duct one end intercommunication is scribbled and is dyed hole and other end intercommunication stock solution fill, be provided with in the honeycomb duct from scribbling and dye the hole to stock solution fill decurrent water.
Through adopting above-mentioned technical scheme, when carrying out centrifugal motion like this, the sample liquid passes through the honeycomb duct directive slide glass from the opening part of stock solution fill, and under the effect of collision force, the sample liquid scatters on the surface of slide glass, forms thin adhesive layer, simultaneously because the effect of water conservancy diversion portion, remaining sample liquid can begin the refluence, flows into the stock solution fill again, can not cause around scribbling the dyeing hole and pile up, and then can not spread along the slide glass, has guaranteed that the background of slide glass is clear.
The invention is further configured to: the box body is internally provided with water absorption paper, the water absorption paper is positioned between the glass slide and the dyeing hole, and a through hole communicated with the dyeing hole is formed in the position, corresponding to the dyeing hole, of the water absorption paper.
By adopting the technical scheme, sometimes the centrifugation time is too long under the unfamiliar or neglected condition of workers, and at the moment, a small amount of sample liquid still diffuses out of the dyeing hole under the frequent scouring action to influence the definition of the slide glass.
The water absorption paper is additionally arranged in the box body and is positioned between the glass slide and the dyeing hole, and the position, corresponding to the dyeing hole, of the water absorption paper is provided with a through hole communicated with the dyeing hole, so that sample liquid diffused from the dyeing hole can be absorbed by the water absorption paper, and the pollution of the glass slide cannot be caused.
The invention is further configured to: the liquid storage bucket is composed of a hemispherical bucket bottom and a horn mouth connected above the bucket bottom, and the bottom of the bucket bottom is lower than the lower end of the flow guide pipe.
By adopting the technical scheme, the horn mouth can enable a worker to conveniently drop or pour the sample liquid into the liquid storage hopper; meanwhile, the hemispherical bucket bottom can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide after being dyed is further ensured.
The invention is further configured to: one or more through air holes are formed in the rear side plate.
Through adopting above-mentioned technical scheme to make the film-making press from both sides when the centrifugation film-making, the gas between slide glass and the box body can discharge fast, makes the effective posterior lateral plate of laminating the box body of slide glass, and then makes the effective area of painting and dyeing that the appearance liquid can effectively adhere to the slide glass.
The invention is further configured to: be provided with the filter screen that can dismantle the connection in the liquid storage fill, the filter screen is the pocket form, the last border of filter screen is taken and is put on the last border of liquid storage fill and the below of filter screen stretches into in the liquid storage fill.
By adopting the technical scheme, the sample solution contains impurities such as mucus, red blood cells and the like, and the impurities can influence the definition of the stained part of the glass slide, so the filter screen is arranged in the liquid storage hopper. The diameter of the mesh of the filter screen is about 6 microns, mucus and red blood cells can be filtered, and then the development of the stained glass slide is clearer.
The invention is further configured to: the upper edge of the rear side plate is provided with a downward sunken U-shaped notch, and the upper edge of the front side plate is lower than or equal to the height of the bottom of the U-shaped notch.
By adopting the technical scheme, the glass slide is conveniently pinched and taken out from the box body by the working personnel.
In conclusion, the invention has the following beneficial effects: when carrying out centrifugal motion, the sample liquid passes through the honeycomb duct directive slide glass from the opening part of stock solution fill, and under the effect of collision force, the sample liquid scatters on the surface of slide glass, forms thin adhesive layer, simultaneously because the effect of water conservancy diversion portion, remaining sample liquid can begin to flow backwards, flows into the stock solution fill again, can not lead to the fact around scribbling the dyeing hole and pile up, and then can not spread along the slide glass, has guaranteed that the background of slide glass is clear.
Drawings
FIG. 1 is an isometric view of a slide holder;
FIG. 2 is a view for showing the structure of the airing hole;
FIG. 3 is a cross-sectional view of a film clip;
fig. 4 is a schematic view of the structure of the extrusion assembly.
In the figure, 1, a box body; 10. a front side plate; 100. coating and dyeing holes; 11. a rear side plate; 110. a U-shaped notch; 112. air holes are formed; 2. a liquid storage hopper; 20. a bell mouth; 21. a bucket bottom; 3. a flow guide pipe; 30. a flow guide part; 31. absorbent paper; 4. a filter screen; 5. an extrusion assembly; 50. a flexible platen; 51. a top rod; 52. a spring; 53. a handle; 54. operation hole, 6, glass slide.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1: a slide making clamp for a centrifugal liquid-based thin-layer cell slide making machine is disclosed, referring to fig. 1 and fig. 2, and comprises a box body 1 for clamping a slide 6, wherein the box body 1 comprises a front side plate 10, a rear side plate 11 and a bottom plate for connecting the front side plate 10 and the rear side plate 11. The upper edge of the rear side plate 11 is provided with a downward-concave U-shaped notch 110, and the upper edge of the front side plate 10 is lower than or equal to the height of the bottom of the U-shaped notch 110. This facilitates the operator to pinch the slide 6 from the cassette 1.
Referring to fig. 3, a transparent staining hole 100 is formed on the front side plate 10 at a position corresponding to the slide 6 to be stained with the staining solution. The liquid storage hopper 2 used for adding sample liquid is arranged at the position opposite to the box body 1, the liquid storage hopper 2 is communicated with the dyeing hole 100 through the guide pipe 3, and the front side plate 10 and the guide pipe 3 are integrally arranged.
The guide pipe 3 is internally provided with a flow guide part 30 which is obliquely arranged, and the flow guide part 30 gradually decreases from the dyeing hole 100 to the liquid storage hopper 2. When carrying out centrifugal motion like this, the sample liquid passes through honeycomb duct 3 directive slide glass 6 from the opening part of liquid storage bucket 2, and under the effect of collision force, the sample liquid scatters on slide glass 6's surface, forms thin adhesion layer, simultaneously because the effect of water conservancy diversion portion 30, remaining sample liquid can begin the refluence, flows in the liquid storage bucket 2 again, can not cause around scribbling dyeing hole 100 and pile up, and then can not spread along slide glass 6, has guaranteed that slide glass 6's background is clear. The straight pipe with the constant diameter and arranged from the dyeing hole 100 to the liquid storage hopper 2 in an inclined downward mode is used as the guide pipe 3, and materials are convenient to obtain.
Referring to FIG. 3, sometimes the centrifugation time is too long due to unfamiliarity or negligence of the worker, and the smear wells 100 still have a small amount of sample fluid spread out under frequent washing action, which affects the clarity of the slide 6.
In order to solve the problem, the absorbent paper 31 is added in the box body 1, the absorbent paper 31 is positioned between the glass slide 6 and the dyeing hole 100, and a through hole communicated with the dyeing hole 100 is formed in the position, corresponding to the dyeing hole 100, of the absorbent paper 31, so that the sample liquid diffused from the dyeing hole 100 can be absorbed by the absorbent paper 31, and the glass slide 6 cannot be polluted.
Referring to fig. 2, one or more air holes 112 are formed in the back side plate 11, so that when the slide is produced by centrifugation, air between the slide 6 and the cassette body 1 can be rapidly discharged, the slide 6 can be effectively attached to the back side plate 11 of the cassette body 1, and the sample liquid can be effectively attached to the effective staining area of the slide 6.
The liquid storage bucket 2 is composed of a hemispherical bucket bottom 21 and a bell mouth 20 connected above the bucket bottom 21. The bell mouth 20 and the hopper bottom 21 are integrally manufactured, and the bell mouth 20 can enable a worker to conveniently drop or pour sample liquid into the liquid storage hopper 2; meanwhile, the hemispherical bucket bottom 21 can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom 21 is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide 6 after being dyed is further ensured.
Referring to fig. 1 and 3, since the sample solution contains impurities such as mucus and red blood cells, which affect the clarity of the stained portion of the slide glass 6, the filter 4 is provided in the reservoir 2. Filter screen 4 is the pocket form, and during the use the last border of filter screen 4 is taken and is put on the opening of stock solution fill 2, and the below stretches into in the stock solution fill 2, and the mesh diameter of filter screen 4 is about 6 microns, can filter mucus and red blood cell, and then makes the video picture after 6 scribbles of slide glass more clear.
Referring to fig. 3 and 4, in actual use, the thickness of the slide 6 is 1mm-1.2mm, which causes the thickness of the slide 6 placed in the box body 1 not to be completely matched with the opening of the box body 1, and a gap exists between the slide 6 and the box body 1, when the centrifugal liquid-based thin-layer cell slide processing machine slightly shakes during use, the slide 6 will also shake with the box body 1, so that the stained cell layer on the slide 6 is scratched by the inner wall of the front side plate 10 of the box body 1 or the water absorption paper 31, and then the development of the slide 6 after being stained is blurred, and the accuracy of analysis and diagnosis is affected.
In order to solve the problem, a pressing assembly 5 for fixing the box body 1 and the slide glass 6 is connected on the front side plate 10, and the pressing assembly 5 comprises a flexible pressing plate 50, a push rod 51, a spring 52, a handle 53 and an operation hole which is arranged on the front side plate 10 and is matched with the push rod 51. The flexible pressing plate 50 is made of rubber in a disc shape, the flexible pressing plate 50 is located between the glass slide 6 and the front side plate 10, one side, protruding along the edge of the flexible pressing plate 50, of the flexible pressing plate is far away from the glass slide 6, the ejector rod 51 is a straight rod, one end, penetrating into the operation hole, of the ejector rod is fixedly connected with the flexible pressing plate 50, one end, penetrating out of the operation hole, of the ejector rod is connected with the handle 53, and the axis of the handle 53 is perpendicular to the axis. The spring 52 is sleeved on the top rod 51 and positioned between the slide glass 6 and the front side plate 10, and the spring 52 is in a pressing state. When the device is used, the spring 52 pushes the flexible pressing disc 50 to extrude the glass slide 6, so that the glass slide 6 is effectively attached to the rear side wall of the box body 1, the glass slide 6 and the box body 1 cannot be scratched, and the staining area of the glass slide 6 can be kept clear. To remove the slide 6, the handle 53 is manually pulled away from the slide 6, thereby retracting the flexible platen 50 and providing sufficient room for the slide 6 to give way.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. The utility model provides a centrifugal liquid-based thin-layer cell pelleter is with system piece clamp, includes box body (1) that is used for the clamping slide glass, is used for storing stock solution fill (2) and honeycomb duct (3) of appearance liquid, and box body (1) includes preceding curb plate (10), posterior lateral plate (11) and is used for connecting the bottom plate of preceding curb plate (10) and posterior lateral plate (11), its characterized in that: a dyeing hole (100) is formed in the position, corresponding to the glass slide, of the front side plate (10), one end of the guide pipe (3) is communicated with the dyeing hole (100), the other end of the guide pipe is communicated with the liquid storage hopper (2), and a guide part (30) which inclines downwards from the dyeing hole (100) to the liquid storage hopper (2) is arranged in the guide pipe (3);
the front side plate (10) is connected with an extrusion assembly (5) for fixing the box body (1) and the glass slide (6), and the extrusion assembly (5) comprises a flexible pressure plate (50), a push rod (51), a spring (52), a handle (53) and an operation hole (54) which is arranged on the front side plate (10) and matched with the push rod (51); the flexible pressing plate (50) is located between the glass slide (6) and the front side plate (10) and one side of the edge of the flexible pressing plate (50) protrudes away from the glass slide (6), the ejector rod (51) penetrates into one end of the operation hole (54) and is fixedly connected with the flexible pressing plate (50), one end of the ejector rod (51) penetrating out of the operation hole (54) is connected with the handle (53), the spring (52) is sleeved on the ejector rod (51) and is located between the glass slide (6) and the front side plate (10), and the spring (52) is in an extrusion state.
2. The slide making clamp for centrifugal liquid-based thin-layer cell slide making machine according to claim 1, wherein: the box body (1) is internally provided with absorbent paper (31), the absorbent paper (31) is positioned between the glass slide and the painting and dyeing hole (100), and a through hole communicated with the painting and dyeing hole (100) is formed in the position, corresponding to the painting and dyeing hole (100), on the absorbent paper (31).
3. The slide making clamp for centrifugal liquid-based thin-layer cell slide making machine according to claim 2, wherein: the liquid storage hopper (2) is composed of a hemispherical hopper bottom (21) and a bell mouth (20) connected above the hopper bottom (21), and the bottom of the hopper bottom (21) is lower than the lower end of the flow guide pipe (3).
4. The slide making clamp for centrifugal liquid-based thin-layer cell slide making machine according to claim 3, wherein: one or more through air holes (112) are formed in the rear side plate (11).
5. The slide making clamp for centrifugal liquid-based thin-layer cell slide making machine according to claim 4, wherein: be provided with filter screen (4) that can dismantle the connection in liquid storage bucket (2), filter screen (4) are the pocket form, the last border of filter screen (4) is taken and is put on the last border of liquid storage bucket (2) and the below of filter screen (4) stretches into in liquid storage bucket (2).
6. The slide making clamp for centrifugal liquid-based thin-layer cell slide maker according to claim 5, wherein: the upper edge of the rear side plate (11) is provided with a downward-sunken U-shaped notch (110), and the upper edge of the front side plate (10) is lower than or equal to the height of the bottom of the U-shaped notch (110).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710370193.3A CN108956218B (en) | 2017-05-23 | 2017-05-23 | Slice making clamp for centrifugal liquid-based thin-layer cell slice making machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710370193.3A CN108956218B (en) | 2017-05-23 | 2017-05-23 | Slice making clamp for centrifugal liquid-based thin-layer cell slice making machine |
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| Publication Number | Publication Date |
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| CN108956218A CN108956218A (en) | 2018-12-07 |
| CN108956218B true CN108956218B (en) | 2021-02-19 |
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| CN201710370193.3A Active CN108956218B (en) | 2017-05-23 | 2017-05-23 | Slice making clamp for centrifugal liquid-based thin-layer cell slice making machine |
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| CN114563243B (en) * | 2022-03-01 | 2024-07-12 | 青岛澜澈生物科技有限公司 | Bacteria collecting container convenient for sample smear |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN205015212U (en) * | 2015-10-17 | 2016-02-03 | 中山众康医疗器械有限公司 | Centrifugal liquid base thin layer cell pelleter presss from both sides with vertical film -making |
| CN205449621U (en) * | 2016-03-31 | 2016-08-10 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
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2017
- 2017-05-23 CN CN201710370193.3A patent/CN108956218B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN205015212U (en) * | 2015-10-17 | 2016-02-03 | 中山众康医疗器械有限公司 | Centrifugal liquid base thin layer cell pelleter presss from both sides with vertical film -making |
| CN205449621U (en) * | 2016-03-31 | 2016-08-10 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
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| CN108956218A (en) | 2018-12-07 |
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