CN112881116A - Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine - Google Patents
Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine Download PDFInfo
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- CN112881116A CN112881116A CN202110036824.4A CN202110036824A CN112881116A CN 112881116 A CN112881116 A CN 112881116A CN 202110036824 A CN202110036824 A CN 202110036824A CN 112881116 A CN112881116 A CN 112881116A
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- container
- slide
- clamping
- based thin
- layer cell
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- 239000007788 liquid Substances 0.000 title claims abstract description 49
- 239000011521 glass Substances 0.000 claims abstract description 38
- 238000003825 pressing Methods 0.000 claims description 16
- 239000011324 bead Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000007689 inspection Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 32
- 238000005119 centrifugation Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sheet making device for a horizontal centrifugal liquid-based thin-layer cell machine, which comprises a container, a glass sheet and a fastener, wherein the fastener is provided with two or more clamping arms and a first fixing groove for placing the glass sheet, the clamping arms are rotatably connected with the fastener, and the side wall of the container is provided with a clamping groove for clamping the clamping arms; the container also comprises a fixing cap, the fixing cap is fixed at the opening of the container, and the fixing cap is used for clamping the side wall of the container by a clamping arm. The slide making clamp for the horizontal centrifugal liquid-based thin-layer cell machine can be better suitable for the horizontal centrifugal liquid-based thin-layer cell machine, only a container, a slide and a fastener are assembled and put into a horizontal centrifugal machine, the manufacturing difficulty is reduced, the efficiency is improved, namely, a sample slide can be quickly manufactured by using the slide making clamp under the premise of not replacing centrifugal machine equipment, and the cost expenditure of an inspection department is saved to the maximum extent.
Description
Technical Field
The invention relates to liquid-based thin-layer cell detection, in particular to a sheet making clamp for a horizontal centrifugal liquid-based thin-layer cell machine.
Background
The liquid-based thin-layer cell detection method comprises the following steps: using a sampling brush, using a preserving fluid to rinse the sampling brush and preserving the sample, shaking and dissolving, preparing a liquid-based thin-layer cell, and observing by a microscope. In the prior art, smear preparation is usually carried out manually, when a specimen is prepared manually, exfoliated cells on a glass slide are difficult to coat uniformly, and in addition, due to the fact that the specimen for sampling the exfoliated cells contains mucus, red blood cells and other impurities, the background of a specimen smear is not clean and clear, so that the observation analysis and diagnosis of a pathologist are influenced.
The invention discloses a slide making clamp for a centrifugal liquid-based thin-layer cell slide making machine, which is published in 12/07/2018 by CN108956218A, and the slide making clamp for the centrifugal liquid-based thin-layer cell slide making machine is characterized by comprising a box body for clamping a glass slide, a liquid storage hopper for storing sample liquid and a flow guide pipe, wherein the box body comprises a front side plate, a rear side plate and a bottom plate for connecting the front side plate and the rear side plate, a coating hole is formed in the position, corresponding to the glass slide, on the front side plate, one end of the flow guide pipe is communicated with the coating hole, the other end of the flow guide pipe is communicated with the liquid storage hopper, and a flow guide part inclining downwards from the coating hole to the liquid storage hopper is arranged in the flow guide pipe. The invention solves the problems that in the use process of the existing slide clamp, too much cell thin layer sample liquid is accumulated at the opening during centrifugation because the slide glass is propped against the opening, and finally the sample liquid is diffused along the slide glass, so that the background of the slide glass is fuzzy and not clean, and the analysis and the diagnosis are influenced.
The above scheme has the following disadvantages: the film making clamp in the patent can only be applied to the cell centrifugal smear machine matched with the film making clamp, but the horizontal centrifugal machine which is widely used at present can not be applied. During operation, the horizontal centrifuge is rotated at high speed to cause a liquid-based flow, see fig. 10 (1-3).
Therefore, designing a sheet making clip suitable for a horizontal centrifuge without changing the structure of the horizontal centrifuge is one of the technical problems to be solved urgently at present.
Disclosure of Invention
The invention aims to provide a slide making clamp for a horizontal centrifugal liquid-based thin-layer cell machine, and solves the problem that the horizontal centrifugal liquid-based thin-layer cell machine cannot be perfectly adapted in the prior art.
In order to realize the purpose of the invention, the invention adopts the following technical scheme: a sheet making device for a horizontal centrifugal liquid-based thin-layer cell machine comprises a container, a glass slide and a fastener, wherein the fastener is provided with two or more clamping arms and a first fixing groove for placing the glass slide, the clamping arms are rotatably connected with the fastener, and the side wall of the container is provided with a clamping groove for clamping the clamping arms; the container also comprises a fixing cap, the fixing cap is fixed at the opening of the container, and the fixing cap is used for clamping the side wall of the container by a clamping arm.
Preferably, the locking cap inner wall is equipped with the internal thread, container outer wall upper end or arm lock upper end are equipped with the external screw thread, arm lock embedding centre gripping inslot is equipped with the external screw thread that accords with container lateral wall order with container outer wall external screw thread same position.
Preferably, the clamping groove is divided into an upper clamping groove and a lower clamping groove, the upper clamping groove and the lower clamping groove are located in different planes, and the clamping arm can completely fit the upper clamping groove and the lower clamping groove.
Preferably, the upper clamping groove is located in a plane closer to the axis of the container than the lower clamping groove.
Preferably, the clamping arm is provided with a pad body used for increasing the attaching degree at the joint of the upper clamping groove and the lower clamping groove.
Preferably, the outer side of the fixing cap is also provided with texture which increases friction force and facilitates rotation of a user.
Preferably, the glass slide further comprises a glass slide, a second fixing groove is formed in the middle of the glass slide, the glass slide is detachably connected with the fastener, and the glass slide can be placed in and fixed in the second fixing groove after being taken out of the first fixing groove.
Compared with the prior art, the sheet making clamp for the horizontal centrifugal liquid-based thin-layer cell machine adopting the technical scheme has the following beneficial effects:
firstly, the sheet making clamp for the horizontal centrifugal liquid-based thin-layer cell machine can be better suitable for the horizontal centrifugal liquid-based thin-layer cell machine, and only a container, a glass sheet and a fastener are assembled and put into a horizontal centrifugal machine, so that the manufacturing difficulty is reduced, and the efficiency is improved.
The slide making clamp can be applied to the existing horizontal centrifuge, namely, on the premise that the centrifuge equipment is not replaced, the sample slide can be quickly made by using the slide making clamp, and the cost expenditure of a laboratory is saved to the maximum extent.
Drawings
FIG. 1 is a schematic view showing the structure of a slide holder of example 2 for a horizontal centrifugal liquid-based thin layer cell machine according to the present invention.
FIG. 2 is a schematic diagram of the slide holder of the horizontal centrifugal liquid-based thin layer cell machine in example 2.
FIG. 3 is a cross-sectional view of a slide holder for a horizontal centrifugal liquid-based thin-layer cell machine in example 2.
FIG. 4 is a cross-sectional view of a slide holder for a horizontal centrifugal liquid-based thin layer cell machine in example 2.
FIG. 5 is a schematic diagram of the slide holder for the horizontal centrifugal liquid-based thin layer cell machine in example 1.
FIG. 6 is a schematic diagram of the slide holder for the horizontal centrifugal liquid-based thin layer cell machine in example 1.
Fig. 7 is a sectional view (when separated) of the pressing mechanism in embodiment 3.
Fig. 8 is a sectional view (at the time of pressing) of the pressing mechanism in embodiment 3.
FIG. 9 is a schematic structural view of a slide glass used in examples 1, 2 and 3.
FIG. 10 is a schematic diagram of the operation of a horizontal centrifugal liquid-based thin layer cell machine in the prior art.
FIG. 11 is a schematic diagram of a slide holder for the horizontal centrifugal liquid-based thin layer cell machine in example 4.
FIG. 12 is a schematic view of the structure of the slide glass of example 4.
FIG. 13 is a schematic view of the structure of the slide glass of example 4.
FIG. 14 is a schematic view of the structure of the slide glass of example 4.
FIG. 15 is a schematic view of the structure of the slide glass of example 4.
FIG. 16 is a schematic view of the structure of the wells of the slide of example 4.
Reference numerals: 1. a container; 11. a clamping groove; 110. an upper clamping groove; 111. a lower clamping groove; 12. a fixing cap; 13. a limit convex ring; 14. filtering paper; 2. glass slide; 3. a fastener; 30. clamping arms; 300. a cushion body; 4. a glass slide; 40. and a second fixing groove.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
Example 1:
the slide making clamp for the horizontal centrifugal liquid-based thin-layer cell machine as shown in fig. 5 to 6 is a slide making device for the horizontal centrifugal liquid-based thin-layer cell machine, and comprises a container 1, a slide 2 and a fastener 3, wherein the fastener 3 is provided with two or more clamping arms 30 and a first fixing groove for placing the slide 2, the clamping arms 30 are rotatably connected with the fastener 3, and the side wall of the container 1 is provided with a clamping groove 11 for clamping the clamping arms 30; the container 1 further comprises a fixing cap 12, the fixing cap 12 is fixed at the opening of the container 1, and the fixing cap 12 is used for clamping the clamping arms 30 to clamp the side wall of the container 1. The open side of the container 1 is provided with a stopper collar 13 for positioning the filter paper 14.
The inner wall of the fixing cap 12 is provided with an internal thread, the upper end of the outer wall of the container 1 or the upper end of the clamping arm 30 is provided with an external thread, and the clamping arm 30 is embedded into the clamping groove 11 and provided with an external thread which accords with the sequence of the side wall of the container 1 at the same position as the external thread of the outer wall of the container 1.
The clamping groove 11 is divided into an upper clamping groove 110 and a lower clamping groove 111, the upper clamping groove 110 and the lower clamping groove 111 are located in different planes, the clamping arm 30 can be completely attached to the upper clamping groove 110 and the lower clamping groove 111, the plane where the upper clamping groove 110 is located is closer to the axis of the container 1 than the lower clamping groove 111, and a pad 300 for increasing the attachment degree is arranged at the joint of the upper clamping groove 110 and the lower clamping groove 111 of the clamping arm 30.
The outside of the fixed cap 12 is also provided with texture which increases friction and facilitates the rotation of the user.
The slide holder for the horizontal centrifugal liquid-based thin-layer cell machine shown in fig. 7 further comprises a slide glass 4, wherein a second fixing groove 40 is formed in the middle of the slide glass 4, the slide glass 2 is detachably connected with the fastener 3, and the slide glass 2 can be placed in and fixed in the second fixing groove 40 after being taken out of the first fixing groove.
The steps used in this example are as follows:
1. the slide 2 is placed in the first fixing groove, the filter paper 14 is sleeved on one side of the limiting convex ring 13, and the container 1 is pressed against the slide 2 to clamp the filter paper 14 firmly.
2. The container 1 is attached to the fastener 3, and the clip arms 30 clip up to the attachment clip grooves 11.
3. At this time, the fixing cap 12 is screwed with the clamping arms 30, and the clamping arms 30 firmly clamp the container 1 and put into a horizontal centrifugal liquid-based thin-layer cell machine.
4. The horizontal centrifugal liquid-based thin layer cell machine is started, the container 1 moves the internal tissue slurry towards the open side of the container 1 with centrifugation, the liquid is filtered through the filter paper 14, and the specimen is left in the middle of the slide 2.
5. After centrifugation, all the specimens to be observed are left on the slide 2, the fixing cap 12 is removed, the clamp arm 30 is opened, and the slide 2 is taken out for observation.
Example 2:
referring to fig. 1 to 4, in the present embodiment, the clamping slot 11 is a linear slot, and the clamping arm 30 is also a linear structure adapted to the clamping slot 11.
Compared with embodiment 1, the present embodiment has the advantages that the taking and taking of the container 1 are easier and the manufacturing cost is relatively lower because the stepped groove and the bent part are not provided.
Example 3:
referring to fig. 7 and 8, in this embodiment, based on embodiment 1 or embodiment 2, a pressing mechanism is added at the position of the fastener 3 and the clamping jaw 30, that is, when the clamping jaw 30 clamps upwards, the pressing mechanism can automatically press the slide 2 on the fastener 3, so that the slide 2 is not easy to shake during movement of the slide holder. When the jaws 30 are deployed, the hold-down mechanism is released, allowing the slide 2 to be separated from the fastener 3, allowing the worker to remove the slide 2.
In this embodiment, the pressing mechanism adopts the pressing bead 5 to cooperate with the movable groove, the movable groove is arranged on the fastener 3, when the clamping jaw 30 is pushed up and clamped, the clamping jaw 30 extrudes the pressing bead 5 towards one side of the slide 2, and the pressing beads 5 on the two sides simultaneously extrude the slide 2 downwards and inwards, so that the slide is firmly fixed on the fastener 3.
Wherein, the pressing bead 5 can be made of plastic (tough material), silica gel rubber (elastic material) or steel (metal material).
Example 4:
as shown in fig. 11, the present example also discloses a slide assembly comprising a slide 4 and the slide holder of example 1.
The middle part of the glass slide 4 is provided with a caulking groove 40, and the glass slide 2 and the fastener 3 can be placed and fixed in the caulking groove 40. The insertion groove 40 is divided into a circular groove 400 and a sector-shaped groove 401 corresponding to the number of the clamping arms 30, the first fixing groove 31 is placed and fixed in the circular groove 400, and the clamping arms 30 can be placed and fixed in the sector-shaped groove 401. Each sector-shaped groove 401 is also provided with one or more limiting protrusions 402 for limiting the position of the clamping arm.
Referring to fig. 11, the sector-shaped slot 401 is divided into an upper slot 401a and a lower slot 401b, the upper slot 401a and the lower slot 401b are not on the same plane, when the clamping arm 30 is placed in the sector-shaped slot 401, the upper segment 30a is located in the upper slot 401a, the lower segment 30b is located in the lower slot 401b, the upper segment 30a is attached to the upper slot 401a, and the top of the upper segment 30a is exposed out of the surface of the slide 4, so that the operator can grasp the slide more easily during taking, and the operator can perform positioning and operation only by touch, thereby facilitating subsequent rotation and taking.
If only the lower segment 30b is provided, the operator is required to take out the clamp arm 30 from the sector groove 401 by picking and digging operation when rotating and taking out.
The procedure used for the tableting assembly in example 3 was as follows:
1. the slide 2 is placed in the first fixing groove, the filter paper 14 is sleeved on the limiting convex ring 13 at the outlet of the container 1, and then the container 1 is pressed down and is pressed against the slide 2, so that the filter paper 14 is firmly squeezed between the container 1 and the slide 2.
2. The container 1 is attached to the fastener 3, and the clip arms 30 clip up to the attachment clip grooves 11.
3. At this time, the fixing cap 12 is screwed with the clamping arms 30, and the clamping arms 30 firmly clamp the container 1 and put into a horizontal centrifugal liquid-based thin-layer cell machine.
4. The horizontal centrifugal liquid-based thin layer cell machine is started, the container 1 moves the internal tissue slurry toward the opening side of the container 1 with the centrifugation, and the specimen is left in the middle of the slide 2.
5. After centrifugation is completed, all the specimens to be observed are left on the slide 2, the fixing cap 12 is removed, and the clamp arms 30 are opened.
6. The clamping arm 30 is placed into the slide 4, the annular fastener 3 is aligned with the embedded groove 40, the slide 2 is positioned above the embedded groove 40, the clamping arm 30 is unfolded and rotated to be clamped into the limiting protrusion 402, and the whole fastener 3 carrying the slide 2 is firmly fixed on the slide 4.
7. After the fixing is completed, the slide 4 is moved so that the slide 2 is aligned with the inspection instrument and can be observed.
The foregoing is a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit of the invention, and these should be considered to be within the scope of the invention.
Claims (9)
1. The utility model provides a horizontal centrifugal liquid-based thin-layer cell machine film-making device which characterized in that: the glass slide fixing device comprises a container (1), a glass slide (2) and a fastener (3), wherein the fastener (3) is provided with two or more clamping arms (30) and a first fixing groove for placing the glass slide (2), the clamping arms (30) are rotatably connected with the fastener (3), and a clamping groove (11) for clamping the clamping arms (30) is formed in the side wall of the container (1);
the container (1) further comprises a fixing cap (12), the fixing cap (12) is fixed at the opening of the container (1), and the fixing cap (12) is used for clamping the side wall of the container (1) through a clamping arm (30).
2. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 1, wherein: the utility model discloses a container, including locking cap (12), locking cap (30), container (1) outer wall upper end or arm lock (30) upper end are equipped with the internal thread, arm lock (30) embedding centre gripping groove (11) are equipped with the external thread that accords with container (1) lateral wall order with container (1) outer wall external thread same position.
3. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 2, wherein: the clamping groove (11) is divided into an upper clamping groove (110) and a lower clamping groove (111), the upper clamping groove (110) and the lower clamping groove (111) are located in different planes, and the clamping arm (30) can be completely attached to the upper clamping groove (110) and the lower clamping groove (111).
4. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 2, wherein: the upper clamping groove (110) is positioned on a plane closer to the axis of the container (1) than the lower clamping groove (111).
5. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 3, wherein: and a pad body (300) used for increasing the attaching degree is arranged at the joint of the upper clamping groove (110) and the lower clamping groove (111) of the clamping arm (30).
6. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 2, wherein: the outer side of the fixed cap (12) is also provided with texture which increases friction force and facilitates rotation of a user.
7. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 2, wherein: the glass slide fixing device is characterized by further comprising a glass slide (4), wherein a second fixing groove (40) is formed in the middle of the glass slide (4), the glass slide (2) is detachably connected with the fastener (3), and the glass slide (2) can be placed into and fixed in the second fixing groove (40) after being taken out of the first fixing groove.
8. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 1, wherein: the fastener (3) is also provided with a pressing mechanism for assisting the fixation of the slide (2).
9. A sheet making apparatus for a horizontal centrifugal liquid-based thin layer cell machine according to claim 8, wherein: the pressing mechanism comprises a movable groove and a pressing bead (5), and the pressing bead (5) is pressed or separated from the glass slide (2) along with the movement of the clamping arm (30);
when the clamping arm (30) is clamped on the container (1), the clamping arm (30) pushes the pressing bead (5) against the slide (2), the slide (2) is pressed in the fastener (3),
when the clamping arm (30) is opened, the clamping arm (30) no longer presses the pressing bead (5), the slide (2) is no longer pressed, and the slide (2) can be easily taken out of the fastener (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110036824.4A CN112881116A (en) | 2021-01-12 | 2021-01-12 | Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110036824.4A CN112881116A (en) | 2021-01-12 | 2021-01-12 | Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine |
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| Publication Number | Publication Date |
|---|---|
| CN112881116A true CN112881116A (en) | 2021-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110036824.4A Pending CN112881116A (en) | 2021-01-12 | 2021-01-12 | Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine |
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| Country | Link |
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| CN (1) | CN112881116A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115532331A (en) * | 2022-09-20 | 2022-12-30 | 上海昂玳医学技术有限公司 | Centrifuge tube, centrifuge and sample processing method |
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| CN205262822U (en) * | 2015-12-12 | 2016-05-25 | 杭州海世嘉生物科技有限公司 | Film -making storehouse base |
| CN106018030A (en) * | 2016-06-22 | 2016-10-12 | 杭州海世嘉生物科技有限公司 | Cell smearing device |
| CN207263510U (en) * | 2017-10-12 | 2018-04-20 | 浙江艾明德生物科技有限公司 | A kind of cell harvestor |
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2021
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040007650A1 (en) * | 2002-07-12 | 2004-01-15 | Kaltek S.R.L. | Disposable support for containers for treating biological samples in cytocentrifuges |
| CN201222018Y (en) * | 2008-03-12 | 2009-04-15 | 郑明� | Centrifugation pellet mill and hanging cradle thereof |
| CN201277923Y (en) * | 2008-09-27 | 2009-07-22 | 程骎 | Clamp for preparing liquid-based thin layer cell |
| CN205015212U (en) * | 2015-10-17 | 2016-02-03 | 中山众康医疗器械有限公司 | Centrifugal liquid base thin layer cell pelleter presss from both sides with vertical film -making |
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