CN112881115B - Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine - Google Patents
Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine Download PDFInfo
- Publication number
- CN112881115B CN112881115B CN202110036815.5A CN202110036815A CN112881115B CN 112881115 B CN112881115 B CN 112881115B CN 202110036815 A CN202110036815 A CN 202110036815A CN 112881115 B CN112881115 B CN 112881115B
- Authority
- CN
- China
- Prior art keywords
- groove
- clamping
- slide
- container
- based thin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 32
- 239000011521 glass Substances 0.000 claims abstract description 34
- 206010064091 Iatrogenic infection Diseases 0.000 abstract description 2
- 206010073310 Occupational exposures Diseases 0.000 abstract description 2
- 231100000675 occupational exposure Toxicity 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 22
- 238000005119 centrifugation Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000003761 preservation solution Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The invention discloses a slice-making component for a horizontal centrifugal liquid-based thin-layer cell machine, which comprises a container, a slide, a fastener and a slide, wherein the fastener is provided with two or more clamping arms and a first fixing groove for placing the slide, the clamping arms are rotatably connected with the fastener, and the side wall of the container is provided with a clamping groove for clamping the clamping arms; the container also comprises a fixing cap, the fixing cap is fixed at the opening of the container, and the fixing cap is used for clamping the side wall of the container by the clamping arm; the middle part of the glass slide is provided with a caulking groove, and the glass slide and the fastening piece can be placed and fixed in the caulking groove. The slide-making assembly for the horizontal centrifugal liquid-based thin-layer cell machine reduces the taking steps of slides, can effectively ensure that samples are not touched and polluted, improves the accuracy of observation results, and can effectively avoid occupational exposure and prevent iatrogenic infection of operators aiming at samples with high infectivity.
Description
Technical Field
The invention relates to liquid-based thin-layer cell detection, in particular to a slice-making assembly for a horizontal centrifugal liquid-based thin-layer cell machine.
Background
The liquid-based thin-layer cell detection steps are as follows: the method comprises the steps of using a sampling brush, rinsing the sampling brush with a preservation solution, preserving the sample, shaking for dissolving, preparing liquid-based thin-layer cell slices, and observing by a microscope. In the prior art, smear preparation is usually carried out manually, and when a specimen is manufactured manually, the exfoliated cells on a glass slide are difficult to uniformly coat, and in addition, because the specimens for sampling the exfoliated cells contain magazines such as mucus, red blood cells and the like, the background of the specimen smear is unclean and clear, thereby influencing the observation, analysis and diagnosis of pathologists.
The invention discloses a tablet clamp for a centrifugal liquid-based thin-layer cell tablet machine, which is disclosed in China patent number CN108956218A published in 12 months 2018 and mainly comprises a box body for clamping a glass slide, a liquid storage hopper for storing sample liquid and a flow guide pipe, wherein the box body comprises a front side plate, a rear side plate and a bottom plate for connecting the front side plate and the rear side plate, a dye coating hole is formed in the position, corresponding to the glass slide, of the front side plate, one end of the flow guide pipe is communicated with the dye coating hole, the other end of the flow guide pipe is communicated with the liquid storage hopper, and a flow guide part which is inclined downwards from the dye coating hole to the liquid storage hopper is arranged in the flow guide pipe. The invention solves the problems that in the use process of the existing slide clamp, too much cell thin layer sample liquid is accumulated at the opening part during centrifugation because the slide glass is propped against the opening part, and finally the sample liquid diffuses along the slide glass, so that the background of the slide glass is blurred and unclean, and the analysis and diagnosis are affected.
The scheme has the following defects: the slice making clamp in the patent is only applicable to a cell centrifugal smear machine matched with the slice making clamp, but a horizontal centrifuge widely used at present cannot be applicable. During operation, the horizontal centrifuge rotates at high speed to cause the liquid base to flow.
Therefore, the design of the tablet clamp applicable to the horizontal centrifuge is one of the technical problems to be solved at present on the basis of not changing the structure of the horizontal centrifuge.
Disclosure of Invention
The invention aims to provide a slice-making component for a horizontal centrifugal liquid-based thin-layer cell machine, which solves the problem that the prior art cannot be perfectly suitable for a water Ping Lixin-type liquid-based thin-layer cell machine.
In order to achieve the above object, the present invention adopts the following technical scheme: the slide-making assembly for the horizontal centrifugal liquid-based thin-layer cell machine comprises a container, a slide, a fastener and a slide glass, wherein the fastener is provided with two or more clamping arms and a first fixing groove for placing the slide glass, the clamping arms are rotatably connected with the fastener, and the side wall of the container is provided with a clamping groove for clamping the clamping arms; the container also comprises a fixing cap, the fixing cap is fixed at the opening of the container, and the fixing cap is used for clamping the side wall of the container by the clamping arm; the middle part of the glass slide is provided with a caulking groove, and the glass slide and the fastening piece can be placed and fixed in the caulking groove.
Preferably, the caulking groove is divided into a circular groove and a sector groove which is matched with the number of the clamping arms, the first fixing groove is arranged and fixed in the circular groove, and the clamping arms can be arranged and fixed in the sector groove.
Preferably, each fan-shaped groove is further provided with one or more limit protrusions for limiting the position of the clamping arm.
Preferably, the inner wall of the fixing cap is provided with internal threads, the upper end of the outer wall of the container or the upper end of the clamping arm is provided with external threads, and the clamping arm is embedded into the clamping groove and is provided with external threads which accord with the sequence of the side wall of the container at the same position of the outer threads of the outer wall of the container.
Preferably, the clamping groove is divided into an upper clamping groove and a lower clamping groove, the upper clamping groove and the lower clamping groove are positioned in different planes, the clamping arm can be completely attached to the upper clamping groove and the lower clamping groove, and the plane where the upper clamping groove is positioned is closer to the axle center of the container than the lower clamping groove.
Preferably, the shape of the vertical section of the fan-shaped groove is matched with the shape of the clamping arm, so that the fan-shaped groove can be completely attached.
Preferably, the connecting part of the upper clamping groove and the lower clamping groove of the clamping arm is provided with a pad body for increasing the fitting degree.
Preferably, the outer side of the fixing cap is also provided with textures which increase friction force and facilitate the rotation of a user. Compared with the prior art, the horizontal centrifugal liquid-based thin-layer cell machine slice-making assembly adopting the technical scheme has the following beneficial effects:
1. The slice-making component for the horizontal centrifugal liquid-based thin-layer cell machine can be better suitable for the horizontal centrifugal liquid-based thin-layer cell machine, and only the container, the glass slide and the fastening piece are assembled and put into the horizontal centrifugal machine, so that the manufacturing difficulty is reduced, and the efficiency is improved.
2. The slide making clamp provided by the application can be suitable for the existing horizontal centrifugal machine, namely, on the premise of not replacing centrifugal machine equipment, a sample slide can be quickly made by using the slide making clamp provided by the application, and the cost expenditure of a laboratory is saved to the greatest extent.
3. After a sample to be observed is filtered by the centrifuge and the filter paper, all solid matters are positioned above the glass slide, at the moment, the fastener with the glass slide can be obtained only by taking down the fixing cap, opening the clamping arm and taking down the container, and then the fixing of the glass slide can be completed only by taking the glass slide with the fastener and then placing the annular bracket part of the fastener into the caulking groove of the glass slide. The middle does not need to use tools such as tweezers to take the slide, and the slide taking device has the following further advantages:
1. on the one hand, the step of taking the slide is reduced, and a worker can move the slide only by holding the clamping arm.
2. On the other hand, as the slide is not contacted with other foreign objects such as fingers or tweezers and the like when moving, the sample can be effectively ensured not to be touched and polluted, and the accuracy of the observation result is improved.
3. Moreover, aiming at a sample with high infectivity, the handheld clamping arm is used for taking and holding the slide, so that the slide is more stable to take and not easy to drop, occupational exposure can be effectively avoided, and iatrogenic infection of operators is prevented.
Drawings
FIG. 1 is a schematic diagram of a slide assembly for a horizontal centrifugal liquid-based thin layer cell machine according to an embodiment of the present invention.
FIG. 2 is a schematic diagram of a slide clamp for a horizontal centrifugal liquid-based thin layer cell machine in an embodiment.
FIG. 3 is a schematic diagram of a slide clamp for a horizontal centrifugal liquid-based thin layer cell machine in an embodiment.
Fig. 4 is the step of loading the slide with the clamp arm and slide of fig. 1 in an embodiment.
Fig. 5 is the step of loading the slide with the clamp arm and slide of the embodiment of fig. 2.
Fig. 6 is the step of loading the slide with the clamp arm and slide of the embodiment of fig. 3.
Fig. 7 is the step of loading the slide with the clamp arm and slide in the embodiment of fig. 4.
Fig. 8 is an enlarged view of a portion a in fig. 6.
Reference numerals: 1. a container; 11. a clamping groove; 110. an upper clamping groove; 111. a lower clamping groove; 12. a fixing cap; 2. a slide; 3. a fastener; 30. a clamp arm; 300. a cushion body; 31. a first fixing groove; 4. a glass slide; 40. a caulking groove; 400. a circular groove; 401. a fan-shaped groove; 402. and a limit protrusion.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
The slice preparation assembly for the horizontal centrifugal liquid-based thin layer cell machine comprises a container 1, a slide 2, a fastener 3 and a slide 4, wherein the fastener 3 is provided with two or more clamping arms 30 and a first fixing groove 31 for placing the slide 2, the clamping arms 30 are rotatably connected with the fastener 3, and a clamping groove 11 for clamping the clamping arms 30 is formed in the side wall of the container 1.
The container 1 further comprises a fixing cap 12, the fixing cap 12 is fixed at the opening of the container 1, textures which increase friction force and facilitate the rotation of a user are further arranged on the outer side of the fixing cap 12, and the fixing cap 12 is used for clamping the side wall of the container 1 by the clamping arms 30;
The middle part of the glass slide 4 is provided with a caulking groove 40, and the glass slide 2 and the fastening piece 3 can be placed and fixed in the caulking groove 40.
The caulking groove 40 is divided into a circular groove 400 and a sector groove 401 corresponding to the number of the clamping arms 30, the first fixing groove 31 is arranged and fixed in the circular groove 400, and the clamping arms 30 can be arranged and fixed in the sector groove 401.
Each of the fan-shaped grooves 401 is further provided with one or more stopper protrusions 402 for defining the position of the clip arms.
The inner wall of the fixing cap 12 is provided with internal threads, the upper end of the outer wall of the container 1 or the upper end of the clamping arm 30 is provided with external threads, and the clamping arm 30 is embedded into the clamping groove 11 and is provided with external threads which are consistent with the sequence of the side wall of the container 1 at the same position of the external threads of the outer wall of the container 1.
The clamping groove 11 is divided into an upper clamping groove 110 and a lower clamping groove 111, the upper clamping groove 110 and the lower clamping groove 111 are located in different planes, wherein the clamping arm 30 is divided into an upper section 30a and a lower section 30b, the upper section 30a is embedded into the upper clamping groove 110, the lower section 30b is embedded into the lower clamping groove 111, the clamping arm 30 can be completely attached to the upper clamping groove 110 and the lower clamping groove 111, and compared with the plane where the upper clamping groove 110 is located, the shape of the vertical section of the lower clamping groove 111, which is closer to the sector groove 401, is matched with the shape of the clamping arm 30, so that the clamping arm can be completely attached.
The upper section 30a is further inward than the lower section 30b, and the upper section 30a is effective to prevent the container 1 from being pulled out during centrifugation when the clamp arms 30 are clamped.
The clamp arm 30 is equipped with the pad body 300 that is used for increasing the laminating degree in last clamp groove 110 and lower clamp groove 111 junction, and the pad body 300 sets up in the upper segment 30a below, and after clamp arm 30 pressed from both sides tightly, upper segment 30a pushes down container 1 through the pad body 300 for container 1 does not produce vibration shake in the centrifugal dehydration in-process, improves the stability of connection.
Referring to fig. 8, the fan-shaped groove 401 is divided into an upper groove 401a and a lower groove 401b, the upper groove 401a and the lower groove 401b are not on the same plane, when the clip arm 30 is placed in the fan-shaped groove 401, the upper section 30a is located in the upper groove 401a, the lower section 30b is located in the lower groove 401b, the upper section 30a is attached to the upper groove 401a, and the top of the upper section 30a exposes the surface of the glass slide 4, so that an operator can grasp the glass slide more easily when taking the glass slide, and the operator can position and operate the glass slide only by touching the glass slide, thereby facilitating the subsequent rotation and taking.
If only the lower segment 30b is used, the operator is required to take out the arm 30 from the fan-shaped groove 401 by a picking and pulling action during rotation and removal.
Specific embodiments of the invention:
1. slide 2 is placed in the first holding tank and container 1 is pressed down against slide 2.
2. The container 1 is attached to the fastener 3, and the clip arms 30 clip up the attachment clip grooves 11.
3. At this time, the fixing cap 12 and the clamping arm 30 are rotated and screwed, the clamping arm 30 firmly clamps the container 1, and the container is placed into the horizontal centrifugal liquid-based thin-layer cell machine.
4. The horizontal centrifugal liquid-based thin layer cell machine starts to operate, the container 1 moves toward the open side of the container 1 with the tissue slurry inside along with the centrifugation, and the sample remains in the middle of the slide 2.
5. After completion of centrifugation, all samples to be observed remain on the slide 2, the fixing cap 12 is removed, and the clamp arm 30 is opened.
6. The clamping arm 30 is put into the glass slide 4 and rotated to be clamped into the limiting protrusion 402, so that the whole fastener 3 carries the glass slide 2 to be firmly fixed on the glass slide 4, and the glass slide can be observed after the fixation is completed.
While the foregoing is directed to the preferred embodiment of the present invention, other and further modifications and improvements may be made by those skilled in the art without departing from the principles of the invention, and such are intended to be considered within the scope of the invention.
Claims (6)
1. A horizontal centrifugal liquid-based thin layer cell machine film-making subassembly, its characterized in that: the device comprises a container (1), a slide (2), a fastener (3) and a slide glass (4), wherein the fastener (3) is provided with two or more clamping arms (30) and a first fixing groove (31) for placing the slide glass (2), the clamping arms (30) are rotatably connected with the fastener (3), and a clamping groove (11) for clamping the clamping arms (30) is formed in the side wall of the container (1);
The container (1) further comprises a fixing cap (12), the fixing cap (12) is fixed at the opening of the container (1), and the fixing cap (12) is used for clamping the side wall of the container (1) by the clamping arm (30); the hand-held clamping arm (30) can move the slide (2);
the middle part of the glass slide (4) is provided with a caulking groove (40), and the glass slide (2) and the fastening piece (3) can be placed and fixed in the caulking groove (40);
The caulking groove (40) is divided into a circular groove (400) and sector grooves (401) which are matched with the number of the clamping arms (30), the first fixing groove (31) is arranged and fixed in the circular groove (400), and the clamping arms (30) can be arranged and fixed in the sector grooves (401);
each fan-shaped groove (401) is also provided with one or more limit protrusions (402) for limiting the position of the clamping arm.
2. The slide assembly for a horizontal centrifugal liquid-based thin layer cell machine according to claim 1, wherein: the inner wall of the fixing cap (12) is provided with internal threads, the upper end of the outer wall of the container (1) or the upper end of the clamping arm (30) is provided with external threads, and the clamping arm (30) is embedded into the clamping groove (11) and is provided with external threads which accord with the sequence of the side walls of the container (1) at the same position with the external threads of the outer wall of the container (1).
3. The slide assembly for a horizontal centrifugal liquid-based thin layer cell machine according to claim 2, wherein: the clamping groove (11) is divided into an upper clamping groove (110) and a lower clamping groove (111), the upper clamping groove (110) and the lower clamping groove (111) are located in different planes, the clamping arm (30) can be completely attached to the upper clamping groove (110) and the lower clamping groove (111), and the plane where the upper clamping groove (110) is located is closer to the axis of the container (1) than the lower clamping groove (111).
4. A tableting assembly according to claim 3, wherein: the shape of the vertical section of the sector groove (401) is matched with the shape of the clamping arm (30), and the sector groove can be completely attached.
5. A tableting assembly according to claim 3, wherein: the joint of the upper clamping groove (110) and the lower clamping groove (111) of the clamping arm (30) is provided with a pad body (300) for increasing the fitting degree.
6. The slide assembly for a horizontal centrifugal liquid-based thin layer cell machine according to claim 1, wherein: the outer side of the fixing cap (12) is also provided with textures which increase friction force and facilitate the rotation of a user.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110036815.5A CN112881115B (en) | 2021-01-12 | Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110036815.5A CN112881115B (en) | 2021-01-12 | Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112881115A CN112881115A (en) | 2021-06-01 |
| CN112881115B true CN112881115B (en) | 2024-07-05 |
Family
ID=
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205262822U (en) * | 2015-12-12 | 2016-05-25 | 杭州海世嘉生物科技有限公司 | Film -making storehouse base |
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205262822U (en) * | 2015-12-12 | 2016-05-25 | 杭州海世嘉生物科技有限公司 | Film -making storehouse base |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112170023B (en) | Centrifugal machine | |
| US20070278154A1 (en) | Cell processing method and cell processing apparatus | |
| CN112881115B (en) | Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine | |
| CN112881116A (en) | Slice making clamp for horizontal centrifugal liquid-based thin-layer cell machine | |
| KR101587975B1 (en) | System and Controlling Method Forautomatic Cell Smear | |
| CN215262692U (en) | Sheet making device | |
| AU2019302550B2 (en) | Automated sample deposition and staining systems and associated methods | |
| CN112881115A (en) | Slice making assembly for horizontal centrifugal liquid-based thin-layer cell machine | |
| CN207882285U (en) | A kind of automatic smear microscopy machine | |
| JPH0862210A (en) | Liquid specimen cell-smearing apparatus and its using method | |
| CN112871473B (en) | Quick film-making subassembly suitable for horizontal centrifuge | |
| CN209086031U (en) | A kind of cell of fast demountable, bacterium specimen collecting device | |
| CN108036994B (en) | Sampling device, using method thereof and equipment for preparing sample | |
| CN209081888U (en) | A kind of cell, bacterium specimen collecting device | |
| CN218674435U (en) | Cell film-making dyeing machine | |
| CN213209664U (en) | Liquid feeding device of centrifugal liquid-based cell film-making | |
| CN114486356B (en) | Sample collection device based on rapid viscosity analyzer | |
| CN205616549U (en) | A device of uncapping for heparin tube | |
| CN210077712U (en) | Urine examination collection system for medical science inspection | |
| CN217738796U (en) | Liquid-based thin-layer cell film-making dyeing device | |
| CN218297754U (en) | Sample quantitative extraction assembly | |
| CN219142397U (en) | Liquid feeding device for centrifugal liquid-based cell flaking | |
| CA3073743C (en) | Waste evacuation apparatus for an automated specimen preparation system | |
| CN219084537U (en) | Simple acid-resistant staining phlegm smear equipment | |
| CN215018023U (en) | Thyroid puncture specimen holder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20240611 Address after: 201400 No. 1599, Pingzhuang West Road, Fengxian District, Shanghai Applicant after: Shanghai Zihanmei Technology Development Co.,Ltd. Country or region after: China Address before: No. 456, Lizhou South Road, Yongkang City, Jinhua City, Zhejiang Province Applicant before: Yongkang Hospital of traditional Chinese Medicine Country or region before: China |
|
| GR01 | Patent grant |