CN108036994B - Sampling device, using method thereof and equipment for preparing sample - Google Patents
Sampling device, using method thereof and equipment for preparing sample Download PDFInfo
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- CN108036994B CN108036994B CN201711226521.9A CN201711226521A CN108036994B CN 108036994 B CN108036994 B CN 108036994B CN 201711226521 A CN201711226521 A CN 201711226521A CN 108036994 B CN108036994 B CN 108036994B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Sampling And Sample Adjustment (AREA)
Abstract
The application discloses sampling device, including body (1), sample part (2), rotary part (3), lid (4), be equipped with the through-hole on lid (4), rotary part (3) detachably with the through-hole is connected with one heart, so that rotary part (3) can be at the through-hole internal rotation, sample part (2) with rotary part (3) are connected, body (1) with lid (4) detachably connects, so that body (1) cover is established the sample part (2) outside, body (1) is including first body, the second body that can dismantle the connection. The application also provides a using method of the sampling device and equipment for making samples. The application provides a sampling device can make sample and diluent intensive mixing, and is efficient, and degree of automation is high, simultaneously, through setting up first body and second body, is convenient for extract the diluent that contains the sample from the second body and detects.
Description
Technical Field
The application relates to the technical field of detection equipment, in particular to a sampling device, a using method thereof and equipment for preparing samples.
Background
The frequent needs of carrying out gynecological examination for patient in clinical collection vagina secretion and do further definite diagnosis, and traditional gynaecology's sample collection mode is for wiping the secretion with the cotton swab, and nevertheless the manual work makes the sample after diluting the processing to the secretion and detects, and this kind of mode efficiency is not high.
In the prior art, there are also a plurality of gynecological sampling devices, which mainly improve how to take gynecological samples, and how to realize automatic sample preparation is not mentioned, so that a new gynecological sampling device needs to be invented to realize automatic sampling.
Disclosure of Invention
To solve the above technical problems, the present invention provides a sampling device; a second object of the present invention is to provide a method for using the above sampling device; a third object of the present invention is to provide an apparatus for taking a sample. According to the sampling device and the using method provided by the invention, the rotating part is arranged, so that a sample and diluent can be fully mixed, and the detection accuracy is improved; simultaneously can cooperate other structures of equipment to carry out the sample preparation automatically, it is efficient, and degree of automation is high, can realize automatic appearance of inhaling, satisfy the use of automated inspection instrument. Meanwhile, the first tube body and the second tube body are arranged, so that the drying operation of the sampling component can be realized; after the rotation finishes, can unpack first body and second body apart, be convenient for draw the diluent that contains the sample from the second body and detect.
The technical scheme provided by the invention is as follows:
the utility model provides a sampling device, includes body, sample parts, rotary part, lid, be equipped with the through-hole on the lid, rotary part detachably with the through-hole is connected with one heart to make rotary part can be in the through-hole internal rotation, sample parts with rotary part connects, the body with lid detachably connects, so that the body cover is established the sample parts outside, the body is including first body, the second body that can dismantle the connection.
Preferably, the rotating component is provided with a liquid adding hole.
Preferably, the second tube is movable back and forth along the tube axis relative to the first tube.
Preferably, the second body can be for first body, can follow body axis back and forth movement specifically is: the first pipe body is provided with a sliding groove, and the second pipe body moves along the sliding groove.
Preferably, the second body can be for first body, can follow body axis back and forth movement specifically is: the first pipe body and the second pipe body are provided with matched threads.
Preferably, one end of the second pipe body, which is far away from the first pipe body, is provided with a liquid collecting part.
Preferably, the liquid collecting part is at least partially made of a soft material.
The invention also provides a method for using the device, which comprises the following steps:
a. sampling: sampling by using a sampling component, then placing the sampling component attached with the sample into the tube body, placing a rotating component connected with the sampling component at the through hole of the cover body, and sleeving the cover body at the opening of the tube body;
b. liquid adding: adding diluent into the tube body through a liquid adding hole arranged on the rotating part;
c. stirring: the driving rotating part rotates to drive the sampling part to rotate so that the sample attached to the sampling part enters the diluent;
d. spin-drying: after stirring, the second pipe body moves towards the direction far away from the first pipe body so as to separate the sampling component from the diluent, the rotating component is driven to rotate again to drive the sampling component to rotate, and residual liquid on the sampling component is thrown out to enter the pipe body;
e. the first body and the second body are separated, and the diluent containing the sample is sucked from the second body and used for detection.
Preferably, in the step e, when the liquid collecting part of the second tube is at least partially made of a soft material, the first tube and the second tube are not separated, the second tube is punctured by using a sampling needle, and a diluent containing a sample is sucked for detection.
The invention also provides equipment for preparing samples, which comprises the sampling device, a stirring device and a sample sucking device, wherein a stirring head of the stirring device is matched with the inner shape of the rotating part and is used for driving the rotating part to rotate; the sample suction device comprises a movement mechanism and a sampling needle, wherein the sampling needle moves under the driving of the movement mechanism and is used for sucking diluent containing a sample in the second pipe body.
The sampling device is provided with the rotating part, the cover body is provided with the through hole, the rotating part is detachably and concentrically connected with the through hole, so that the rotating part can rotate in the through hole, and the rotating part is connected with the sampling part, so that the rotating part rotates to drive the sampling part to rotate relative to the pipe body, the sampling part and diluent in the pipe body generate relative motion, and a sample attached to the sampling part enters the diluent in the pipe body to be collected. The invention can realize the sampling and diluting work of the sample by arranging the cover body with the through hole, the rotating part and the sampling part, and has simple structure, small volume and simple and convenient operation. In addition, the tube body can be a first tube body and a second tube body which are detachably connected, the second tube body contains diluent and a sample entering the diluent, and after the rotation is finished, the first tube body and the second tube body are disassembled, so that the diluent can be conveniently taken out of the tube bodies, and the detection can be conveniently carried out if the diluent is sucked or poured out. First body, second body can adopt multiple modes such as threaded connection, buckle connection, interference fit, sliding connection to connect.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a sampling device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of a sampling device (the sampling device is in contact with a diluent) according to an embodiment of the present invention;
FIG. 3 is a schematic view of a sampling device according to an embodiment of the present invention (the sampling device is not in contact with the diluent);
FIG. 4 is a first schematic diagram illustrating a sampling device sucking a second tube by sampling according to an embodiment of the present invention;
FIG. 5 is a second schematic view of a sampling device for sampling a second tube according to an embodiment of the present invention;
reference numerals: 1-a pipe body; 2-a sampling member; 3-a rotating part; 4-a cover body; 5-liquid adding holes; 1 a-a first tube; 1 b-a second tube; c-a sampling needle; d-a stirring head of the stirring device.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
As shown in fig. 1 to 5, an embodiment of the present invention provides a sampling device, including a tube 1, a sampling member 2, a rotating member 3, and a cover 4, where the cover 4 is provided with a through hole, the rotating member 3 is detachably and concentrically connected with the through hole, so that the rotating member 3 can rotate in the through hole, the sampling member 2 is connected with the rotating member 3, the tube 1 is detachably connected with the cover 4, so that the tube 1 is sleeved outside the sampling member 2, and the tube 1 includes a first tube and a second tube that are detachably connected.
The sampling device is provided with a rotating part 3, a through hole is formed in a cover body 4, the rotating part 3 is detachably and concentrically connected with the through hole, so that the rotating part 3 can rotate in the through hole, and the rotating part 3 is connected with a sampling part 2, so that the rotating part 3 rotates to drive the sampling part 2 to rotate relative to a pipe body 1, the sampling part 2 and diluent in the pipe body 1 generate relative motion, and samples attached to the sampling part 2 enter the diluent in the pipe body 1 to be collected. The rotating part 3 is detachably and concentrically connected with the through hole, that is, the rotating part 3 is matched with the through hole of the cover 4 in shape and size, so that the rotating part 3 can be placed in the through hole of the cover 4 and is concentrically arranged with the through hole, and the rotating part 3 can rotate in the through hole of the cover 4. The invention can realize the sampling and diluting work of the sample by arranging the cover body 4 with the through hole, the rotating part 3 and the sampling part 2, and has simple structure, small volume and simple and convenient operation. In addition, the tube 1 of the present invention can be configured as a first tube and a second tube which are detachably connected, the second tube contains the diluent and the sample entering the diluent, and after the rotation is completed, the first tube and the second tube are disassembled, so that the diluent can be conveniently taken out from the tube 1, and the detection can be conveniently performed if the diluent is sucked or poured out. First body, second body can adopt multiple modes such as threaded connection, buckle connection, interference fit, sliding connection to connect.
According to the device, the sampling part 2 is any one of a cotton swab, a sponge and a brush head, so that the sampling part 2 can be used for sampling human samples such as gynecological samples conveniently.
Preferably, the sampling member 2 is disposed concentrically with the rotating member 3, and the rotating member 3 rotates to concentrically rotate the sampling member 2, so that the sample attached to the sampling member 2 enters the diluent. The sampling member 2 and the rotating member 3 may be integrally formed, or may be separately manufactured and then connected to each other for use.
Preferably, the rotating component 3 is provided with a liquid adding hole 5.
Preferably, the rotating member 3 is provided with an addition hole 5 for adding a diluent for dissolving or reacting with the sample into the tube 1.
Preferably, the second tube is movable back and forth along the tube axis relative to the first tube.
Preferably, the second body and the first body are matched with each other through a buckle or other modes, so that the second body can move back and forth relative to the first body along the axis direction of the second body. When the tube body rotates, the second tube body is firstly selected to move towards the first tube body, so that the sampling part 2 in the tube body 1 is close to the bottom of the second tube body, and a sample on the sampling part 2 enters the diluent and is fully contacted with the diluent in the rotating process; then remove the second body to the direction of keeping away from first body, make sampling component 2 break away from the diluent, rotatory sampling component 2 once more this moment, make the effect of centrifugal force of adnexed diluent and sample on the sampling component 2 thrown away along with, get into in the body 1, in order to improve the acquisition rate to the sample, make the experimental result more accurate, and can avoid taking apart first body and second body after, the liquid on the sampling component 2 can drip, produce the influence to testing process or instrument life-span.
Preferably, the second body can be for first body, can follow body axis back and forth movement specifically is: the first pipe body is provided with a sliding groove, and the second pipe body moves along the sliding groove.
Preferably, the second body can be for first body, can follow body axis back and forth movement specifically is: the first pipe body and the second pipe body are provided with matched threads.
The second body can be along body axis reciprocating motion for first body, can realize through spout or the screw thread that mutually supports, or other structures. Preferably, a protrusion may be provided at an end of the sliding groove or the screw thread to prevent the first pipe and the second pipe from being separated from each other when the first pipe and the second pipe do not need to be disassembled. The size setting of lug is in suitable scope for first body can not break away from each other with the second body when ordinary use, and when first body and second body need be unpacked apart, exert external force, can make second body break away from with first body, be convenient for draw the diluent in the second body.
Preferably, one end of the second pipe body, which is far away from the first pipe body, is provided with a liquid collecting part.
The one end that the second body was kept away from first body is equipped with album liquid portion, collects the sample of diluent and entering diluent in album liquid portion, and the follow-up diluent that contains the sample of drawing in following from the second body of being convenient for detects. The liquid collecting part can be in any shape, and is generally provided with a shape with gradually reduced inner diameter, such as a cone shape, so that the diluent and the sample are concentrated and the subsequent taking operation is convenient.
Preferably, the liquid collecting part is at least partially made of a soft material.
As preferred, can set up the at least part of collection liquid portion into the soft materials that can puncture, like the flexible glue material, after the operation of rotatory collection sample is accomplished, impale collection liquid portion through syringe needle or collecting head, absorb out the sample in collection liquid portion, and need not take apart first body and second body, can realize the collection of sample, avoid polluting, can reduce hand labor by machine operation simultaneously.
The invention also provides a method for using the device, which comprises the following steps:
a. sampling: sampling by using the sampling component 2, then placing the sampling component 2 attached with the sample into the tube body 1, placing the rotating component 3 connected with the sampling component 2 at the through hole of the cover body 4, and sleeving the cover body 4 at the opening of the tube body 1;
b. liquid adding: adding diluent into the tube body 1 through a liquid adding hole 5 arranged on the rotating part 3;
c. stirring: the driving rotating part 3 rotates to drive the sampling part 2 to rotate, so that the sample attached to the sampling part 2 enters the diluent;
d. spin-drying: after stirring, the second pipe body moves towards the direction far away from the first pipe body so as to separate the sampling component 2 from the diluent, the rotating component 3 is driven to rotate again to drive the sampling component 2 to rotate, and residual liquid on the sampling component 2 is thrown out and enters the pipe body 1;
e. the first body and the second body are separated, and the diluent containing the sample is sucked from the second body and used for detection.
Preferably, in the step e, when the liquid collecting part of the second tube is at least partially made of a soft material, the first tube and the second tube are not separated, the second tube is punctured by using a sampling needle, and a diluent containing a sample is sucked for detection.
According to the sampling device and the using method provided by the invention, the rotating part is arranged, so that a sample and diluent can be fully mixed, and the detection accuracy is improved; meanwhile, the sample can be automatically manufactured by matching with other structures of equipment, the efficiency is high, the automation degree is high, automatic sample suction can be realized, the use of an automatic detection instrument is met, meanwhile, the first tube body and the second tube body are arranged, the first tube body and the second tube body are mutually far away after stirring is finished, the sampling device 2 is separated from diluent in the tube bodies and rotates again, so that samples attached to the sampling device 2 are thrown out to enter the tube bodies 1 and then enter the diluent, on one hand, the samples can enter the diluent to ensure the accuracy of detection results, on the other hand, when the first tube body and the second tube body are disassembled, no liquid drips on the sampling part 2, and the cleanness and the service life of the instrument are influenced; after the spin-drying is finished, the first tube body and the second tube body can be detached, and the diluent containing the sample can be conveniently extracted from the second tube body for detection. When the liquid collecting part of the second tube body is made of soft materials at least partially, the first tube body and the second tube body can be separated or not separated, the second tube body is punctured by using a sampling needle, and diluent containing a sample is sucked for detection.
Preferably, after the stirring is completed (i.e. the rotation step of immersing the sampling part 2 in the diluent is completed), the operation of separating the first tube from the second tube may be automatically completed by a machine, and the first tube and the second tube are separated from each other after the stirring by providing a protrusion or other structures on the second tube and providing a clamping and moving device in cooperation with the machine, etc. By the arrangement, the sampling device provided by the invention can be used for automatically processing the sample and absorbing the sample, so that the automatic detection is facilitated, and the detection efficiency is improved.
The sampling member 2 and the rotating member 3 in the present invention may be integrally formed, or may be separately manufactured and connected for use. Usually sold to the consumer, the sampling member 2 is connected to the rotary member 3 and can be used directly. The cover 4 may be connected to the sampling member 2 and the rotating member 3 at the time of sale, or may be attached by an operator at the time of use, as necessary.
The invention also provides equipment for preparing samples, which comprises the sampling device, a stirring device and a sample sucking device, wherein a stirring head of the stirring device is matched with the inner shape of the rotating part 3 and is used for driving the rotating part 3 to rotate; the sample suction device comprises a movement mechanism and a sampling needle, wherein the sampling needle moves under the driving of the movement mechanism and is used for sucking diluent containing a sample in the second pipe body.
The sampling device provided by the invention can be used as equipment for preparing samples by matching with a stirring device and a sample sucking device. The stirring head of the stirring device is matched with the inner shape of the rotating part 3, and the stirring head is preferably provided with a bulge matched with the liquid adding hole 5 of the rotating part 3, so that the rotating part 3 can be fixed during rotation. The sampling needle and the motion mechanism can be arranged below the sampling device or at other positions, and the movement of the motion mechanism enables the sampling needle to sample the diluent containing the sample in the sampling device.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A sampling device used for matching with an automatic detection instrument to prepare a sample is characterized by comprising a tube body (1), a sampling component (2), a rotating component (3) and a cover body (4), wherein a through hole is formed in the cover body (4), the rotating component (3) is detachably and concentrically connected with the through hole so that the rotating component (3) can rotate in the through hole, the sampling component (2) is connected with the rotating component (3), the tube body (1) is detachably connected with the cover body (4) so that the tube body (1) is sleeved outside the sampling component (2), the tube body (1) comprises a first tube body and a second tube body which are detachably connected, the second tube body can move back and forth along the axis of the tube body relative to the first tube body so that the sampling component (2) can be fully contacted with a diluent in the rotating process, and is separated from the diluent during centrifugation.
2. Device according to claim 1, characterized in that the rotating member (3) is provided with a filling hole (5).
3. The device of claim 1, wherein the second tube is movable back and forth along the tube axis relative to the first tube, in particular: the first pipe body is provided with a sliding groove, and the second pipe body moves along the sliding groove.
4. The device of claim 1, wherein the second tube is movable back and forth along the tube axis relative to the first tube, in particular: the first pipe body and the second pipe body are provided with matched threads.
5. A device according to any one of claims 3 to 4, wherein the end of the second tube remote from the first tube is provided with a liquid collection portion.
6. The device of claim 5, wherein the liquid trap is at least partially made of a soft material.
7. A method of using the apparatus according to any one of claims 2-6, comprising the steps of:
a. sampling: sampling by using the sampling component (2), then placing the sampling component (2) attached with the sample into the tube body (1), placing the rotating component (3) connected with the sampling component (2) at the through hole of the cover body (4), and sleeving the cover body (4) at the opening of the tube body (1);
b. liquid adding: diluent is added into the tube body (1) through a liquid adding hole (5) arranged on the rotating part (3);
c. stirring: the driving rotating part (3) rotates to drive the sampling part (2) to rotate, so that the sample attached to the sampling part (2) enters the diluent;
d. spin-drying: after stirring, the second pipe body moves towards the direction far away from the first pipe body so as to separate the sampling component (2) from the diluent, the rotating component (3) is driven to rotate again to drive the sampling component (2) to rotate, and residual liquid on the sampling component (2) is thrown out and enters the pipe body (1);
e. the first body and the second body are separated, and the diluent containing the sample is sucked from the second body and used for detection.
8. The method of claim 7, wherein in step e, when the liquid trap portion of the second tube is at least partially made of a soft material, the first tube is not separated from the second tube, and the second tube is punctured with a sampling needle to aspirate the diluent containing the sample for detection.
9. An apparatus for preparing a sample, comprising a sampling device according to any one of claims 1 to 6, further comprising a stirring device, a sample sucking device, a stirring head of the stirring device matching with the inner shape of the rotating member (3) for driving the rotating member (3) to rotate; the sample suction device comprises a movement mechanism and a sampling needle, wherein the sampling needle moves under the driving of the movement mechanism and is used for sucking diluent containing a sample in the second pipe body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711226521.9A CN108036994B (en) | 2017-11-29 | 2017-11-29 | Sampling device, using method thereof and equipment for preparing sample |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711226521.9A CN108036994B (en) | 2017-11-29 | 2017-11-29 | Sampling device, using method thereof and equipment for preparing sample |
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| CN108036994A CN108036994A (en) | 2018-05-15 |
| CN108036994B true CN108036994B (en) | 2020-12-22 |
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| CN111743579B (en) * | 2020-07-07 | 2023-05-30 | 德阳市人民医院 | Infectious department protection type pathogen sample collection device |
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| CA1340208C (en) * | 1988-06-09 | 1998-12-15 | James F. Monthony | Biological sample collection and transport device |
| JP3416739B2 (en) * | 1994-02-09 | 2003-06-16 | 佐藤 勝恒 | Test equipment with sample collection equipment |
| DE29608616U1 (en) * | 1996-05-11 | 1996-08-01 | Sbf Wasser Und Umwelt Zweignie | Mixing vessel |
| US6653147B2 (en) * | 2000-03-31 | 2003-11-25 | Neogen Corporation | Apparatus and method for chemiluminescent assays |
| US8268634B2 (en) * | 2007-11-29 | 2012-09-18 | Ameditech, Inc. | Fluid sample collecting and analyzing apparatus and method |
| JP5197324B2 (en) * | 2008-11-26 | 2013-05-15 | 株式会社東芝 | Automatic analyzer |
| CN201765132U (en) * | 2010-07-07 | 2011-03-16 | 吴白平 | Disposable feces quantitative collecting and preprocessing device |
| CN202210038U (en) * | 2011-08-15 | 2012-05-02 | 长沙协大生物科技有限公司 | Sample blending collection tube of excrement analytic system |
| CN103728172B (en) * | 2014-01-02 | 2017-02-15 | 爱威科技股份有限公司 | Method and equipment for making defecate detection solution, and diafiltration device thereof |
| US10576468B2 (en) * | 2014-01-20 | 2020-03-03 | Abogen, Inc. | Devices, solutions and methods for sample collection |
| WO2015172250A1 (en) * | 2014-05-14 | 2015-11-19 | Dna Genotek Inc. | Device for collecting, transporting and storing biomolecules from a biological sample |
| CN203861264U (en) * | 2014-05-28 | 2014-10-08 | 甄艳梅 | Obstetrics and gynecology sampler |
| CN203943692U (en) * | 2014-07-03 | 2014-11-19 | 刘玉珍 | Automatic adjustable sampler for cervical cell tool |
| CN104792567B (en) * | 2015-04-30 | 2018-03-02 | 珠海市银科医学工程有限公司 | It is a kind of new to adopt just and tachysynthesis detection means and its operating method |
| ES2891524T3 (en) * | 2015-07-24 | 2022-01-28 | Novel Microdevices Inc | Sample processing device comprising magnetic and mechanical drive elements using linear or rotational motion |
| CN206235467U (en) * | 2016-11-11 | 2017-06-09 | 宁波司迈尔医疗科技有限公司 | A kind of sampler |
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