CN108956219B - Film making clamp for liquid-based thin-layer cell film making machine - Google Patents
Film making clamp for liquid-based thin-layer cell film making machine Download PDFInfo
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- CN108956219B CN108956219B CN201710370736.1A CN201710370736A CN108956219B CN 108956219 B CN108956219 B CN 108956219B CN 201710370736 A CN201710370736 A CN 201710370736A CN 108956219 B CN108956219 B CN 108956219B
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- slide
- liquid
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- box body
- glass
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The invention discloses a slide making clamp for a liquid-based thin-layer cell slide making machine, which has the technical scheme that the slide making clamp comprises a box body for clamping a slide glass, a liquid storage hopper for storing sample liquid and a flow guide pipe, wherein the box body comprises a front side plate, a rear side plate and a bottom plate for connecting the front side plate and the rear side plate, a painting hole is formed in the position, corresponding to the slide glass, on the front side plate, a painting hole is formed, one end of the flow guide pipe is communicated with the painting hole, the other end of the flow guide pipe is communicated with the liquid storage hopper, and an extrusion assembly for fixing the box body and the. The invention solves the problems that the thickness of a glass slide is not completely matched with the opening of a box body in the using process of the existing slide making clamp, and when a centrifugal liquid-based thin-layer cell slide making machine slightly shakes in the using process, the glass slide also shakes with the box body, so that a cell layer dyed on the glass slide is scratched by the inner wall of the front side plate of the box body, the development of the glass slide after being dyed is fuzzy, and the accuracy of analysis and diagnosis is influenced.
Description
Technical Field
The invention relates to liquid-based thin-layer cell detection, in particular to a slide making clamp for a liquid-based thin-layer cell slide making machine.
Background
The liquid-based thin-layer cell detection method comprises the following steps: sampling by using a sampling brush, rinsing the sampling brush by using a preserving fluid and preserving the sample, shaking and dissolving, flaking liquid-based thin-layer cells and observing by using a microscope. In the prior art, smear preparation is usually carried out manually, when a specimen is prepared manually, exfoliated cells on a glass slide are difficult to coat uniformly, and in addition, due to the fact that the specimen for sampling the exfoliated cells contains mucus, red blood cells and other impurities, the background of a specimen smear is not clean and clear, so that the observation analysis and diagnosis of a pathologist are influenced.
Chinese utility model patent with publication number CN202393969U discloses a liquid-based thin layer cell slide making clamp for solving the above problems, the use of the slide making clamp is more convenient, but the slide making clamp is in actual use, the thickness of the slide glass is between 1mm-1.2mm, this thickness that causes the slide glass put into the box body does not match completely with the opening of the box body, there is a gap between the slide glass and the box body, when the centrifugal liquid-based thin layer cell slide making machine slightly shakes in the use process, the slide glass can also shake with the box body, and then the cell layer that dyes on the slide glass receives the scratch of the front side plate inner wall of the box body, the development after the slide glass is painted is blurred, and the accuracy of analysis and diagnosis is affected.
Disclosure of Invention
The invention aims to provide a slide making clamp for a liquid-based thin-layer cell slide making machine, which has the characteristic of avoiding rubbing of a glass slide and a box body in a centrifugal process.
The above object of the present invention is achieved by the following technical solutions: the utility model provides a liquid-based thin-layer cell slide maker is with system slide holder, includes the box body that is used for the clamping slide glass, is used for storing the stock solution fill and the honeycomb duct of appearance liquid, and the box body includes preceding curb plate, posterior lateral plate and is used for connecting the bottom plate of preceding curb plate and posterior lateral plate, the hole of dying has been seted up to scribbling in the position department that corresponds the slide glass on the preceding curb plate, honeycomb duct one end intercommunication is scribbled and is dyed hole and other end intercommunication liquid storage fill, be connected with the extrusion subassembly that is used for fixed box body and slide glass on the preceding.
By adopting the technical scheme, when the device is used, the extrusion assembly fixes the positions of the box body and the glass slide, so that the glass slide and the box body cannot slide relatively, the rubbing phenomenon cannot occur, and the staining area of the glass slide can be kept clear.
The invention is further configured to: the extrusion subassembly includes flexible pressure disk, ejector pin, spring, handle and sets up the handle hole of cooperation ejector pin on the preceding curb plate, and flexible pressure disk is located between slide glass and the preceding curb plate, and the ejector pin is the straight-bar, and the ejector pin penetrates the one end and the flexible pressure disk fixed connection of handle hole and wears out the one end and the handle fixed connection of handle hole, and the axis of handle is perpendicular with the axis of ejector pin, and the spring housing is on the ejector pin and be located between slide glass and the preceding curb plate, and the spring is in the extrusion state.
By adopting the technical scheme, when the flexible glass slide sticking device is used, the spring pushes the flexible pressing disc to extrude the glass slide, so that the glass slide is effectively attached to the rear side plate of the box body, the glass slide and the box body cannot be scraped, and the smearing area of the glass slide can be kept clear. When the slide glass is to be taken out, the hand of a person pulls the handle in the direction departing from the slide glass, so that the flexible pressing disc retreats, and the slide glass is provided with enough space for abdicating.
The invention is further configured to: the box body is internally provided with water absorption paper, the water absorption paper is positioned between the glass slide and the dyeing hole, and a through hole communicated with the dyeing hole is formed in the position, corresponding to the dyeing hole, of the water absorption paper.
By adopting the technical scheme, sometimes the centrifugation time is too long under the unfamiliar or neglected condition of workers, and at the moment, a small amount of sample liquid still diffuses out of the dyeing hole under the frequent scouring action to influence the definition of the slide glass.
The water absorption paper is additionally arranged in the box body and is positioned between the glass slide and the dyeing hole, and the position, corresponding to the dyeing hole, of the water absorption paper is provided with a through hole communicated with the dyeing hole, so that sample liquid diffused from the dyeing hole can be absorbed by the water absorption paper, and the pollution of the glass slide cannot be caused.
The invention is further configured to: the liquid storage bucket is composed of a hemispherical bucket bottom and a horn mouth connected above the bucket bottom, and the bottom of the bucket bottom is lower than the lower end of the flow guide pipe.
By adopting the technical scheme, the horn mouth can enable a worker to conveniently drop or pour the sample liquid into the liquid storage hopper; meanwhile, the hemispherical bucket bottom can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide after being dyed is further ensured.
The invention is further configured to: one or more through air holes are formed in the rear side plate.
Through adopting above-mentioned technical scheme to make the film-making press from both sides when the centrifugation film-making, the gas between slide glass and the box body can discharge fast, makes the effective posterior lateral plate of laminating the box body of slide glass, and then makes the effective area of painting and dyeing that the appearance liquid can effectively adhere to the slide glass.
The invention is further configured to: be provided with the filter screen that can dismantle the connection in the liquid storage fill, the filter screen is the pocket form, the last border of filter screen is taken and is put on the last border of liquid storage fill and the below of filter screen stretches into in the liquid storage fill.
By adopting the technical scheme, the sample solution contains impurities such as mucus, red blood cells and the like, and the impurities can influence the definition of the stained part of the glass slide, so the filter screen is arranged in the liquid storage hopper. The diameter of the mesh of the filter screen is about 6 microns, mucus and red blood cells can be filtered, and then the development of the stained glass slide is clearer.
The invention is further configured to: the upper edge of the rear side plate is provided with a downward sunken U-shaped notch, and the upper edge of the front side plate is lower than or equal to the height of the bottom of the U-shaped notch.
By adopting the technical scheme, the glass slide is conveniently pinched and taken out from the box body by the working personnel.
In conclusion, the invention has the following beneficial effects: the extrusion assembly fixes the position of the box body and the position of the slide glass, so that the slide glass and the box body cannot slide relatively, the rubbing phenomenon cannot occur, and the smearing area of the slide glass can be kept clear.
Drawings
FIG. 1 is an isometric view of a slide holder;
FIG. 2 is a view for showing the structure of the airing hole;
FIG. 3 is a cross-sectional view of a film clip;
fig. 4 is a schematic view of the structure of the extrusion assembly.
In the figure, 1, a box body; 10. a front side plate; 100. coating and dyeing holes; 11. a rear side plate; 110. a U-shaped notch; 112. air holes are formed; 2. a liquid storage hopper; 20. a bell mouth; 21. a bucket bottom; 3. a flow guide pipe; 30. a flow guide part; 31. absorbent paper; 4. a filter screen; 5. an extrusion assembly; 50. a flexible platen; 51. a top rod; 52. a spring; 53. a handle; 54. an operation hole; 6. a slide.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1: the utility model provides a liquid-based thin-layer cell slide making machine is with system piece holder, is including the box body 1 that is used for clamping slide 6, and box body 1 includes preceding curb plate 10, posterior lateral plate 11 and is used for connecting the bottom plate of preceding curb plate 10 and posterior lateral plate 11. The upper edge of the rear side plate 11 is provided with a downward-concave U-shaped notch 110, and the upper edge of the front side plate 10 is lower than or equal to the height of the bottom of the U-shaped notch 110. This facilitates the operator to pinch the slide 6 from the cassette 1.
The front side plate 10 is provided with a through dyeing hole 100 corresponding to the position of the slide glass 6 needing to be coated with the dyeing liquid. A liquid storage hopper 2 for adding sample liquid is arranged at the position opposite to the box body 1, and the liquid storage hopper 2 is communicated with the dyeing hole 100 through a flow guide pipe 3.
The guide pipe 3 is internally provided with a flow guide part 30 which is obliquely arranged, and the flow guide part 30 gradually decreases from the dyeing hole 100 to the liquid storage hopper 2. When carrying out centrifugal motion like this, the sample liquid passes through honeycomb duct 3 directive slide glass 6 from the opening part of liquid storage bucket 2, and under the effect of collision force, the sample liquid scatters on slide glass 6's surface, forms thin adhesion layer, simultaneously because the effect of water conservancy diversion portion 30, remaining sample liquid can begin the refluence, flows in the liquid storage bucket 2 again, can not cause around scribbling dyeing hole 100 and pile up, and then can not spread along slide glass 6, has guaranteed that slide glass 6's background is clear. The straight pipe with the constant diameter and arranged from the dyeing hole 100 to the liquid storage hopper 2 in an inclined downward mode is used as the guide pipe 3, and materials are convenient to obtain.
Sometimes, the centrifugation time is too long due to unfamiliarity or negligence of workers, and at this time, a small amount of sample liquid still spreads out from the staining holes 100 under the frequent washing action, which affects the clarity of the slide 6.
In order to solve the problem, the absorbent paper 31 is added in the box body 1, the absorbent paper 31 is positioned between the glass slide 6 and the dyeing hole 100, and a through hole communicated with the dyeing hole 100 is formed in the position, corresponding to the dyeing hole 100, of the absorbent paper 31, so that the sample liquid diffused from the dyeing hole 100 can be absorbed by the absorbent paper 31, and the glass slide 6 cannot be polluted.
One or more air holes 112 are formed in the rear side plate 11, so that when the slide holder is used for centrifugal slide making, gas between the glass slide 6 and the box body 1 can be rapidly discharged, the glass slide 6 is effectively attached to the rear side plate 11 of the box body 1, and then sample liquid can be effectively attached to an effective dyeing area of the glass slide 6.
The liquid storage bucket 2 is composed of a hemispherical bucket bottom 21 and a bell mouth 20 connected above the bucket bottom 21. The bell mouth 20 and the hopper bottom 21 are integrally manufactured, and the bell mouth 20 can enable a worker to conveniently drop or pour sample liquid into the liquid storage hopper 2; meanwhile, the hemispherical bucket bottom 21 can contain more sample liquid, so that the method is suitable for the condition that the sample liquid needs to be filled subsequently due to less cell content in the sample liquid, and the hemispherical bucket bottom 21 is more convenient for backflow of the redundant sample liquid, so that the definition of the glass slide 6 after being dyed is further ensured.
Meanwhile, the sample solution contains impurities such as mucus, red blood cells and the like, and the impurities can influence the definition of the stained part of the glass slide 6, so the filter screen 4 is arranged in the liquid storage bucket 2. Filter screen 4 is the pocket form, and during the use the last border of filter screen 4 is taken and is put on the opening of stock solution fill 2, and the below stretches into in the stock solution fill 2, and the mesh diameter of filter screen 4 is about 6 microns, can filter mucus and red blood cell, and then makes the video picture after 6 scribbles of slide glass more clear.
Because the thickness of the glass slide 6 is between 1mm and 1.2mm in practical use, the thickness of the glass slide 6 put into the box body 1 is not completely matched with the opening of the box body 1, and a gap exists between the glass slide 6 and the box body 1, when the centrifugal liquid-based thin-layer cell pelleter slightly shakes in the use process, the glass slide 6 also shakes with the box body 1, so that a cell layer stained on the glass slide 6 is scratched by the inner wall of the front side plate 10 of the box body 1 or the water absorption paper 31, and the development of the stained glass slide 6 is blurred, and the accuracy of analysis and diagnosis is affected.
In order to solve this problem, a pressing assembly 5 for fixing the cassette body 1 and the slide 6 is connected to the front side plate 10, and the pressing assembly 5 includes a flexible platen 50, a push rod 51, a spring 52, a handle 53, and an operation hole 54 formed in the front side plate 10 to engage with the push rod 51. The flexible pressing plate 50 is in a disc shape and made of rubber, the flexible pressing plate 50 is located between the glass slide 6 and the front side plate 10, one side, protruding on the edge of the flexible pressing plate 50, of the flexible pressing plate 50 deviates from the glass slide 6, the ejector rod 51 is a straight rod, one end, penetrating into the operation hole 54, of the ejector rod is fixedly connected with the flexible pressing plate 50, one end, penetrating out of the operation hole 54, of the ejector rod is connected with the handle 53, and the axis of the handle 53 is perpendicular to the axis. The spring 52 is sleeved on the top rod 51 and positioned between the slide glass 6 and the front side plate 10, and the spring 52 is in a pressing state. When the device is used, the spring 52 pushes the flexible pressing disc 50 to extrude the glass slide 6, so that the glass slide 6 is effectively attached to the rear side plate 11 of the box body 1, the glass slide 6 and the box body 1 cannot be scratched, and a staining area of the glass slide 6 can be kept clear. To remove the slide 6, the handle 53 is manually pulled away from the slide 6, thereby retracting the flexible platen 50 and providing sufficient room for the slide 6 to give way.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. The utility model provides a liquid-based thin-layer cell pelleter is with system piece clamp, includes box body (1) that is used for clamping slide glass (6), liquid storage bucket (2) and honeycomb duct (3) that are used for storing appearance liquid, box body (1) including preceding curb plate (10), posterior lateral plate (11) and be used for connecting the bottom plate of preceding curb plate (10) and posterior lateral plate (11), the hole (100) of painting has been seted up to the position department that corresponds slide glass (6) on preceding curb plate (10), honeycomb duct (3) one end intercommunication is painted hole (100) and other end intercommunication liquid storage bucket (2), its characterized in that: the front side plate (10) is connected with an extrusion assembly (5) for fixing the box body (1) and the glass slide (6);
the extrusion assembly (5) comprises a flexible pressing plate (50), a push rod (51), a spring (52), a handle (53) and an operation hole (54) which is arranged on a front side plate (10) and is matched with the push rod (51), the flexible pressing plate (50) is positioned between the glass slide (6) and the front side plate (10), the push rod (51) is a straight rod, one end of the push rod (51) penetrating into the operation hole (54) is fixedly connected with the flexible pressing plate (50) and penetrates out of the operation hole (54), the end of the push rod (51) is fixedly connected with the handle (53), the axis of the handle (53) is perpendicular to the axis of the push rod (51), the spring (52) is sleeved on the push rod (51) and positioned between the glass slide (6) and the front side plate (10), and the spring (52) is in.
2. The slide making holder for liquid-based thin-layer cell slide making machine according to claim 1, wherein: the box body (1) is internally provided with absorbent paper (31), the absorbent paper (31) is positioned between the glass slide (6) and the painting and dyeing hole (100), and a through hole communicated with the painting and dyeing hole (100) is formed in the position, corresponding to the painting and dyeing hole (100), on the absorbent paper (31).
3. The slide making holder for liquid-based thin-layer cell slide making machine according to claim 2, wherein: the liquid storage hopper (2) is composed of a hemispherical hopper bottom (21) and a bell mouth (20) connected above the hopper bottom (21), and the bottom of the hopper bottom (21) is lower than the lower end of the flow guide pipe (3).
4. The slide making holder for liquid-based thin-layer cell slide making machine according to claim 3, wherein: one or more through air holes (112) are formed in the rear side plate (11).
5. The slide making holder for liquid-based thin-layer cell slide making machine according to claim 4, wherein: be provided with filter screen (4) that can dismantle the connection in liquid storage bucket (2), filter screen (4) are the pocket form, the last border of filter screen (4) is taken and is put on the last border of liquid storage bucket (2) and the below of filter screen (4) stretches into in liquid storage bucket (2).
6. The slide making holder for liquid-based thin-layer cell slide making machine according to claim 5, wherein: the upper edge of the rear side plate (11) is provided with a downward-sunken U-shaped notch (110), and the upper edge of the front side plate (10) is lower than or equal to the height of the bottom of the U-shaped notch (110).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710370736.1A CN108956219B (en) | 2017-05-23 | 2017-05-23 | Film making clamp for liquid-based thin-layer cell film making machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710370736.1A CN108956219B (en) | 2017-05-23 | 2017-05-23 | Film making clamp for liquid-based thin-layer cell film making machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108956219A CN108956219A (en) | 2018-12-07 |
| CN108956219B true CN108956219B (en) | 2021-02-19 |
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| CN201710370736.1A Active CN108956219B (en) | 2017-05-23 | 2017-05-23 | Film making clamp for liquid-based thin-layer cell film making machine |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110631877B (en) * | 2019-10-28 | 2022-04-12 | 宁波美生医疗器材有限公司 | Tripping and separating mechanism for liquid-based cell film production |
| WO2022149975A1 (en) * | 2021-01-05 | 2022-07-14 | Cheong Mun Shun | Apparatus and method for automatically preparing thin-layer slides |
| CN112871473B (en) * | 2021-01-12 | 2022-06-24 | 永康市中医院 | Quick film-making subassembly suitable for horizontal centrifuge |
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| CN101246096A (en) * | 2008-03-12 | 2008-08-20 | 郑明� | Centrifugal pellet mill and hanging cradle used for the same |
| CN201653782U (en) * | 2010-04-20 | 2010-11-24 | 南京健邦锦源医疗仪器有限公司 | Liquid-based thin-layer cytologic preparation device |
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN205015212U (en) * | 2015-10-17 | 2016-02-03 | 中山众康医疗器械有限公司 | Centrifugal liquid base thin layer cell pelleter presss from both sides with vertical film -making |
| CN205449604U (en) * | 2016-01-04 | 2016-08-10 | 北京诚智光辉科技有限公司 | Cell film -making subassembly |
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2017
- 2017-05-23 CN CN201710370736.1A patent/CN108956219B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246096A (en) * | 2008-03-12 | 2008-08-20 | 郑明� | Centrifugal pellet mill and hanging cradle used for the same |
| CN201653782U (en) * | 2010-04-20 | 2010-11-24 | 南京健邦锦源医疗仪器有限公司 | Liquid-based thin-layer cytologic preparation device |
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN205015212U (en) * | 2015-10-17 | 2016-02-03 | 中山众康医疗器械有限公司 | Centrifugal liquid base thin layer cell pelleter presss from both sides with vertical film -making |
| CN205449604U (en) * | 2016-01-04 | 2016-08-10 | 北京诚智光辉科技有限公司 | Cell film -making subassembly |
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| CN108956219A (en) | 2018-12-07 |
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