CN114563243B - Bacteria collecting container convenient for sample smear - Google Patents

Bacteria collecting container convenient for sample smear Download PDF

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Publication number
CN114563243B
CN114563243B CN202210189799.8A CN202210189799A CN114563243B CN 114563243 B CN114563243 B CN 114563243B CN 202210189799 A CN202210189799 A CN 202210189799A CN 114563243 B CN114563243 B CN 114563243B
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China
Prior art keywords
slide
glass
gland
cup
sputum cup
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CN114563243A (en
Inventor
丁洁
吴林
李阳兴
庄嫄
庄泉洁
刘洪英
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Qingdao Lanchen Biotechnology Co ltd
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Qingdao Lanchen Biotechnology Co ltd
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Priority to CN202210189799.8A priority Critical patent/CN114563243B/en
Publication of CN114563243A publication Critical patent/CN114563243A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a bacteria collecting container convenient for sample smear, which adopts a cup body integrating a sputum cup and a glass slide base body, a flow passage hole communicated with the outer vertical surface of the glass slide base body is arranged on the side wall of the sputum cup, an L-shaped slot and a limit groove are arranged on the outer vertical surface of the glass slide base body, and when the glass slide is arranged on the glass slide base body on the cup body, one surface of the glass slide is covered on the flow passage hole; the invention also adopts the slide glass gland with the elastic pressing ring arranged on the slide glass base, the slide glass gland is buckled on the slide glass base, and the O-shaped ring on the flow passage hole of the external vertical surface of the slide glass and the slide glass base is pressed by the elastic pressing ring of the slide glass gland to realize the sealing of the sputum cup. When the automatic smear device is used, a sample is put into the sputum cup, and after centrifugation, the sample is coated on a glass slide through a runner hole of the sputum cup, so that the automatic smear process of the sample is realized.

Description

Bacteria collecting container convenient for sample smear
Technical Field
The invention relates to a detection instrument in the technical field of medical biology, in particular to a bacteria collecting container convenient for sample smear.
Background
At present, the morphological examination of pathogenic bacteria, mold, ova or cells and other microorganisms requires scientific researchers and medical staff to manually coat samples on glass slides, and then perform the work of fixing, staining, microscopic examination and the like.
With the development of medical instrument technology, the development of the automatic machine instead of artificial dyeing and microscopic examination is realized, and the machine has the advantages of stable operation and good effect. The problems are that the automatic smearing process on the glass slide is still to be solved, the existing automatic smearing process has common problems of complex use, easy occurrence of liquid leakage and difficult maintenance, and the automatic smearing process becomes a bottleneck for realizing the whole process of automatic smearing, dyeing and film reading.
Because most samples used in scientific research institutions and hospitals contain pathogenic microorganisms, ensuring the sealing operation of the smear process and no liquid leakage in the whole smear process is a key for ensuring the biological safety of scientific research personnel and medical personnel. Especially after the new coronavirus outbreak, the working efficiency of medical staff is improved, the time of manual input is reduced, the direct contact of high-infectivity samples is avoided, the detection time is shortened, the accuracy of the detection result is ensured, and therefore, the automatic smear wish is more urgent.
In order to realize smear automation, ensure that the smear process realizes closed operation, ensure that the sample does not leak and the bacteria collecting effect is good, the design of a bacteria collecting container for the sample smear is a key which can be realized in the automatic smear process.
Disclosure of Invention
The invention aims at overcoming the defects of the prior art and provides a bacteria collecting container convenient for sample smear, which adopts a cup body formed by integrating a sputum cup and a slide glass base body, wherein a runner hole penetrating with the outer vertical surface of the slide glass base body is horizontally arranged on the side wall of the sputum cup, an L-shaped slot and a limit groove are arranged on the outer vertical surface of the slide glass base body, and when a slide glass is arranged between the L-shaped slot of the slide glass base body and the limit groove of the bottom edge on the cup body, one surface of the slide glass is covered on the runner hole; according to the invention, the slide glass gland with the elastic pressing ring is arranged on the slide glass base, the slide glass gland is buckled on the outer vertical surface of the slide glass base on the cup body, the slide glass gland is hinged with the slide glass base, and the O-shaped ring on the flow passage hole of the outer vertical surface of the slide glass and the slide glass base is pressed by the elastic pressing ring of the slide glass gland to realize the sputum cup sealing.
When the automatic smear device is used, a sample is put into the sputum cup, and after dilution and centrifugation, the sample is coated on a glass slide through a runner hole of the sputum cup, so that the automatic smear process of the sample is realized.
The technical scheme for realizing the aim of the invention is as follows:
A bacteria collecting container convenient for sample smear is characterized by comprising a cup body, a sputum cup rotating cover, a silica gel pad, a glass slide gland, a glass slide, an O-shaped ring and a torsion spring;
The cup body is formed by integrating a columnar sputum cup and a rectangular glass slide seat body attached to the outer side of the columnar sputum cup; the upper opening of the sputum cup is provided with external threads, the side wall of the sputum cup is horizontally provided with a runner hole communicated with the outer vertical surface of the slide glass seat, the orifice of the runner hole is provided with a sealing ring groove, the two side edges of the outer vertical surface of the slide glass seat are provided with L-shaped slots in the vertical direction, the bottom edge of the outer vertical surface of the slide glass seat is provided with a limit groove in the horizontal direction, the two side edges of the upper plane of the slide glass seat are provided with buckles, and the two side surfaces of the slide glass seat are symmetrically provided with shaft seats and torsion spring limiting blocks;
the slide glass gland is in a rectangular shell shape, clamping holes are formed in two sides of the upper plane of the slide glass gland, hinge holes are symmetrically formed in two side faces of the slide glass gland, and an elastic pressing ring corresponding to the position of the sealing ring groove on the slide glass seat body is arranged on the inner side of the slide glass gland;
the sputum cup spiral cover is provided with an internal thread, and the top surface of the sputum cup spiral cover is provided with a round hole;
The silica gel pad is a round pad with a convex circle in the middle;
the O-shaped ring is arranged in a sealing ring groove of the glass slide seat body on the cup body;
the glass slide is arranged in the limiting grooves on the two sides of the glass slide seat body on the cup body;
The slide glass gland is buckled on the outer vertical surface of the slide glass seat body on the cup body, the hinge shaft hole of the gland is hinged with the shaft seat of the slide glass seat body, the clamping hole of the slide glass gland is clamped with the clamping buckle of the slide glass seat body, and the elastic pressing ring of the slide glass gland presses the slide glass and the O-shaped ring;
The torsion springs are arranged on the shaft seat of the slide glass seat body, one end of each torsion spring is arranged on the torsion spring limiting block, and the other end of each torsion spring is contacted with the inner side of the slide glass gland;
the silica gel pad is arranged in the sputum cup spiral cover, and the convex circle of the silica gel pad protrudes outwards from the round hole of the sputum cup spiral cover;
The sputum cup spiral cover is buckled on the sputum cup, and the internal thread of the sputum cup spiral cover is meshed with the external thread of the upper opening of the sputum cup.
And a sealing ring groove is arranged on the orifice of the runner hole on the cup body and is positioned on the outer vertical surface of the slide glass seat body.
The invention adopts a cup body which is formed by integrating a sputum cup and a glass slide base body, a flow passage hole which is communicated with the outer vertical surface of the glass slide base body is horizontally arranged on the side wall of the sputum cup, and when a glass slide is arranged between an L-shaped slot of the glass slide base body and a limit groove at the bottom edge on the cup body, one surface of the glass slide is covered on the flow passage hole; according to the invention, the slide glass gland with the elastic pressing ring is arranged on the slide glass seat body, the gland is buckled on the outer vertical surface of the slide glass seat body on the cup body, and the slide glass is pressed to the O-shaped ring of the flow passage orifice of the side wall of the sputum cup through the elastic pressing ring of the gland to realize the sealing of the sputum cup;
When the automatic smear machine is used, a sample is put into a sputum cup, diluted and centrifuged, and then the sample is coated on a glass slide through a runner hole of the sputum cup, so that the automatic smear process of the sample is realized.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
fig. 2 is a schematic diagram of the a-direction structure of fig. 1.
Detailed Description
Referring to fig. 1 and 2, the invention comprises a cup body 1, a sputum cup spiral cover 2, a silica gel pad 3, a slide glass gland 4, a slide glass 5, an O-shaped ring 6 and a torsion spring 7;
The cup body 1 is formed by integrating a columnar sputum cup 11 and a rectangular glass slide base 12 attached to the outer side of the columnar sputum cup; the upper opening of the sputum cup 11 is provided with an external thread 111, the side wall of the sputum cup 11 is horizontally provided with a runner hole 113 communicated with the outer vertical surface of the glass slide seat body 12, the orifice of the runner hole 113 is provided with a sealing ring groove 114, the two sides of the outer vertical surface of the glass slide seat body 12 are provided with L-shaped slots 121 in the vertical direction, the bottom edge is provided with a limit groove 122 in the horizontal direction, the two sides of the upper plane of the glass slide seat body 12 are provided with buckles 123, and the two sides of the glass slide seat body 12 are symmetrically provided with shaft seats 124 and torsion spring limiting blocks 125;
the slide gland 4 is in a rectangular shell shape, clamping holes 41 are formed in two sides of the upper plane of the slide gland 4, hinge holes 42 are symmetrically formed in two side surfaces of the slide gland 4, and an elastic pressing ring 43 corresponding to the sealing ring groove 114 on the slide seat 12 is arranged on the inner side of the slide gland 4;
The sputum cup spiral cover 2 is provided with an internal thread, and the top surface of the sputum cup spiral cover 2 is provided with a round hole 21; the silica gel pad 3 is a circular pad with a convex circle 31 in the middle;
the O-shaped ring 6 is arranged in a sealing ring groove 114 of the glass slide seat body 12 on the cup body 1;
The glass slide 5 is arranged in the L-shaped slots 121 on the two sides of the glass slide seat body 12 and the limit grooves 122 on the bottom edge of the glass slide seat body 12 on the glass body 1.
Referring to fig. 1 and 2, the slide gland 4 is buckled on the outer vertical surface of the slide seat 12 on the cup 1, the hinge shaft hole 42 of the slide gland 4 is hinged with the shaft seat 124 of the slide seat 12, the clamping hole 41 of the slide gland 4 is clamped with the clamping buckle 123 of the slide seat 12, and the elastic pressing ring 43 of the slide gland 4 presses the slide 5 and the O-ring 6;
the torsion springs 7 are two, the torsion springs 7 are arranged on the shaft seat 124 of the slide glass seat body 12, one end of each torsion spring 7 is arranged on the torsion spring limiting block 125, and the other end of each torsion spring is contacted with the inner side of the slide glass gland 4;
the silica gel pad 3 is arranged in the sputum cup spiral cover 2, and the convex circle 31 of the silica gel pad 3 protrudes outwards from the round hole 21 of the sputum cup spiral cover 2;
The sputum cup spiral cover 2 is buckled on the sputum cup 11, and the internal thread of the sputum cup spiral cover 2 is meshed with the external thread 111 of the upper opening of the sputum cup 11.
Referring to fig. 1 and 2, a sealing ring groove 114 is disposed on the opening of the flow passage hole 113 on the cup body 1, and the sealing ring groove 114 is located on the outer vertical surface of the slide seat 12.
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The embodiment of the invention comprises the following steps:
Referring to fig. 1 and 2, the invention adopts a cup body 1 formed by integrating a sputum cup 11 and a slide glass base 12, a runner hole 113 penetrating through the outer vertical surface of the slide glass base 12 is horizontally arranged on the side wall of the sputum cup 11, an L-shaped slot 121 and a limit groove 122 are arranged on the outer vertical surface of the slide glass base 12, and when a slide glass 5 is arranged between the L-shaped slot 121 of the slide glass base 12 and the limit groove 122 on the bottom edge of the cup body 1, one surface of the slide glass 5 is covered on the runner hole 113;
The slide gland 4 with the elastic pressing ring 43 is arranged on the slide seat body 12, the slide gland 4 is buckled on the outer vertical surface of the slide seat body 12 on the cup body 1, the slide gland 4 is hinged with the slide seat body 12, and the elastic pressing ring 43 of the slide gland 4 presses the slide 5 and the O-shaped ring 6 to realize the sealing of the flow passage hole 113.
The invention is matched with a smear machine, and the smear machine is used for vibrating and centrifugally smearing a bacteria-collecting container with a collected sample.
Referring to fig. 1 and 2, the specific working process is as follows, the sputum cup 11 with the collected sample is diluted, and when diluted, a quantitative diluent is injected into the sputum cup 11 by a syringe through a convex circle 31 protruding outwards from a silica gel pad 3 on a sputum cup spiral cover 2;
Placing the invention in a smear machine, starting the smear machine, vibrating the smear machine to make the sample and the diluent present a uniform sample suspension in the sputum cup 11; after the vibration is completed, the smear machine performs high-speed centrifugation, and the diluted sample suspension in the sputum cup 11 is thrown out through the runner hole 113 on the side wall of the sputum cup 11 and is adhered to the glass slide 5 covered with the runner hole 113.
Referring to fig. 1 and 2, after centrifugation, a mechanical arm or a manual press is used to press the buckle 123 of the slide seat 12 to separate the clamping hole 41 of the slide gland 4 from the buckle 123, under the action of the torsion spring 7, the slide gland 4 buckled on the slide seat 12 on the cup body 1 rotates around the shaft seat 124 to spring, the elastic pressing ring 43 for pressing the slide 5 simultaneously releases the slide 5, the slide 5 is taken out from the L-shaped slot 121 of the slide seat 12 by the mechanical arm or the manual, and then the work such as fixing, dyeing, microscopic examination and the like is performed, so that the automatic smearing and inspection process of samples are realized, and the full sealing, no leakage and automatic detection of samples are realized in a laboratory.
Referring to fig. 1 and 2, in order to ensure good sealing of a sample, a silica gel pad 3 is arranged in a sputum cup screw cap 2, the sputum cup screw cap 2 is buckled on a sputum cup 11, and internal threads of the sputum cup screw cap 2 are meshed with external threads 111 of an upper opening of the sputum cup 11;
Referring to fig. 1 and 2, in order to facilitate dilution of a sample in a sputum cup 11, the invention adopts a round hole 21 arranged on the top surface of a sputum cup spiral cover 2, a convex circle 31 arranged on a silica gel pad 3, the silica gel pad 3 is arranged in the sputum cup spiral cover 2, the convex circle 31 of the silica gel pad 3 protrudes outwards from the round hole 21 of the sputum cup spiral cover 2, and a quantitative diluent is injected into the sputum cup 11 by a syringe through the convex circle 31 protruding outwards on the sputum cup spiral cover 2, so that the dilution of the sample under a sealed condition is realized.
Referring to fig. 1 and 2, in order to ensure the sealing between the slide 5 and the runner hole 113, the present invention has a sealing ring groove 114 on the orifice of the runner hole 113, an O-ring 6 is disposed in the sealing ring groove 114, and an elastic pressing ring 43 corresponding to the position of the sealing ring groove 114 on the slide seat 12 is disposed on the inner side of the slide pressing cover 4, when the slide pressing cover 4 is buckled on the outer vertical surface of the slide seat 12 on the cup body 1, the elastic pressing ring 43 of the slide pressing cover 4 presses the slide 5 and the O-ring 6, so as to realize the sealing between the surface of the slide 5 and the runner hole 113, and ensure that the sample suspension diluted in the sputum cup 11 is thrown out and adhered on the slide 5 covered with the runner hole 113 when the smear machine performs high-speed centrifugation, and no liquid leakage occurs.
Referring to fig. 1 and 2, the hinge shaft hole 42 of the slide gland 4 is hinged with the shaft seat 124 of the slide seat 12, and the clamping hole 41 of the slide gland 4 is clamped with the clamping buckle 123 of the slide seat 12, so that the slide gland 4 is buckled on the outer vertical surface of the slide seat 12 on the cup body 1, the elastic pressing ring 43 of the slide gland 4, the slide 5 and the O-shaped ring 6 are tightly bonded, the sealing among the slide 5, the O-shaped ring 6 and the runner hole 113 is ensured, the elastic pressing ring 43 uniformly and stably applies force to the slide 5, and the slide 5 does not loosen due to severe shaking such as vibration or centrifugation, and liquid leakage is avoided.
Referring to fig. 1 and 2, in order to facilitate the use of a manipulator and enable a slide gland 4 of a slide seat 12 buckled on a cup body 1 to automatically spring out around a shaft seat 124, the shaft seat 124 of the slide seat 12 is provided with a torsion spring 7, one end of the torsion spring 7 is arranged on a torsion spring limiting block 125, and the other end of the torsion spring 7 is contacted with the inner side of the slide gland 4; when the buckle 123 of the slide glass seat body 12 is pressed to separate the clamping hole 41 of the slide glass gland 4 from the buckle 123, the slide glass gland 4 buckled on the slide glass seat body 12 on the cup body 1 automatically bounces off around the shaft seat 124 under the action of the torsion spring 7.

Claims (2)

1. The bacteria collecting container is characterized by comprising a cup body (1), a sputum cup spiral cover (2), a silica gel pad (3), a glass slide gland (4), a glass slide (5), an O-shaped ring (6) and a torsion spring (7);
The cup body (1) is formed by integrating a columnar sputum cup (11) and a rectangular glass slide base body (12) attached to the outer side of the columnar sputum cup; the upper opening of the sputum cup (11) is provided with an external thread (111), the side wall of the sputum cup (11) is horizontally provided with a runner hole (113) communicated with the outer vertical surface of the glass slide base (12), the orifice of the runner hole (113) is provided with a sealing ring groove (114), the two side edges of the outer vertical surface of the glass slide base (12) are provided with L-shaped slots (121) in the vertical direction, the bottom edge is provided with a limit groove (122) in the horizontal direction, the two side edges of the upper plane of the glass slide base (12) are provided with buckles (123), and the two side surfaces of the glass slide base (12) are symmetrically provided with shaft seats (124) and torsion spring limit blocks (125);
The slide gland (4) is in a rectangular shell shape, clamping holes (41) are formed in two sides of the upper plane of the slide gland (4), hinge holes (42) are symmetrically formed in two side surfaces of the slide gland (4), and an elastic pressing ring (43) corresponding to the position of a sealing ring groove (114) on the slide seat body (12) is arranged on the inner side of the slide gland (4);
the sputum cup spiral cover (2) is provided with an internal thread, and the top surface of the sputum cup spiral cover (2) is provided with a round hole (21);
the silica gel pad (3) is a round pad with a convex circle (31) in the middle;
The O-shaped ring (6) is arranged in a sealing ring groove (114) of the glass slide seat body (12) on the cup body (1);
The glass slide (5) is arranged in L-shaped slots (121) on two sides of the glass slide base (12) and limit grooves (122) on the bottom edge of the glass slide base (12) on the glass body (1);
The slide glass gland (4) is buckled on the outer vertical surface of the slide glass base (12) on the cup body (1), the hinge shaft hole (42) of the gland (4) is hinged with the shaft seat (124) of the slide glass base (12), the clamping hole (41) of the slide glass gland (4) is clamped with the clamping buckle (123) of the slide glass base (12), and the elastic pressing ring (43) of the slide glass gland (4) presses the slide glass (5) and the O-shaped ring (6);
The two torsion springs (7) are arranged, the torsion springs (7) are arranged on the shaft seat (124) of the slide glass seat body (12), one end of each torsion spring (7) is arranged on the torsion spring limiting block (125), and the other end of each torsion spring is contacted with the inner side of the slide glass gland (4);
The silica gel pad (3) is arranged in the sputum cup spiral cover (2), and the convex circle (31) of the silica gel pad (3) protrudes outwards from the round hole (21) of the sputum cup spiral cover (2);
The sputum cup spiral cover (2) is buckled on the sputum cup (11), and the internal thread of the sputum cup spiral cover (2) is meshed with the external thread (111) of the upper opening of the sputum cup (11).
2. A bacteria collecting container for facilitating sample smear according to claim 1, characterized in that a sealing ring groove (114) is arranged on the orifice of the runner hole (113) on the cup body (1), and the sealing ring groove (114) is positioned on the outer vertical surface of the slide seat body (12).
CN202210189799.8A 2022-03-01 2022-03-01 Bacteria collecting container convenient for sample smear Active CN114563243B (en)

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CN114563243B true CN114563243B (en) 2024-07-12

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102539211A (en) * 2012-01-09 2012-07-04 广州三瑞医疗器械有限公司 Device and method for making liquid-based cytological smear in one step
CN108956218A (en) * 2017-05-23 2018-12-07 北京诚智光辉科技有限公司 Centrifugal Thinprep pap test pelleter is pressed from both sides with film-making

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110132088A (en) * 2010-06-01 2011-12-07 서울대학교병원 (분사무소) Chamber for cell smearing and chamber set having the same
CN215179015U (en) * 2021-04-14 2021-12-14 易普森智慧医疗(佛山)有限公司 Centrifugal liquid-based cell pelleter is with system piece holder

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102539211A (en) * 2012-01-09 2012-07-04 广州三瑞医疗器械有限公司 Device and method for making liquid-based cytological smear in one step
CN108956218A (en) * 2017-05-23 2018-12-07 北京诚智光辉科技有限公司 Centrifugal Thinprep pap test pelleter is pressed from both sides with film-making

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