CN221078670U

CN221078670U - Indirect immunofluorescence method sample adding device

Info

Publication number: CN221078670U
Application number: CN202322633407.5U
Authority: CN
Inventors: 黄庆; 李文涵
Original assignee: Hangzhou Oumeng Weiyi Medical Laboratory Co ltd
Current assignee: Hangzhou Oumeng Weiyi Medical Laboratory Co ltd
Filing date: 2023-09-27
Publication date: 2024-06-04
Anticipated expiration: 2033-09-27

Abstract

The application relates to an indirect immunofluorescence sample adding device, which comprises: the slide glass comprises a plurality of slide glass units, a slide glass support and a sample adding plate group, wherein the slide glass support forms a plurality of grooves which are arranged in parallel with each other, and the slide glass units are detachably inserted and fixed on the slide glass support through the grooves; the sample adding plate group comprises a sample adding frame part and a plurality of sample adding units which are integrally arranged with the sample adding frame part, wherein each sample adding unit is rectangular, one end part of the short side of the rectangle of each sample adding unit is fixed on the sample adding frame part, the other end part of the rectangle of each sample adding unit is a free end, and the plurality of sample adding units are arranged in parallel at equal intervals and correspond to the grooves; the surface shape of each slide glass unit is correspondingly arranged with each sample adding unit, and the slide glass units are used for covering samples to be tested at the corresponding positions of the sample adding units under the state that the slide glass support and the sample adding plate group are oppositely interleaved. The indirect immunofluorescence sample adding device can improve the accuracy and efficiency of immunofluorescence experiments.

Description

Indirect immunofluorescence method sample adding device

Technical Field

The application relates to the technical field of biological experimental equipment, in particular to an indirect immunofluorescence sample adding device.

Background

Immunofluorescence is a method that combines immunological methods with fluorescent labeling techniques for studying the intracellular distribution of specific protein antigens.

At present, when the indirect immunofluorescence method is manually detected in a laboratory, a method of directly adding samples on fluorescent slides is generally adopted, and the main operation steps of the immunofluorescence method sample adding experiment are as follows:

Preparation: the sample plate was checked to see if the reaction area was hydrophilic and the periphery hydrophobic, if not, wiped clean with a wet paper towel, and then the slide was removed from the kit and the package opened when equilibrated to room temperature.

Diluting the sample: samples were diluted with wash buffer PBS-Tween (Phosphate-buffered SALINE WITH TWEEN) according to the user's experimental design, and mixed well before each experiment.

Sample incubation: and respectively adding 30ul diluted samples into each reaction zone of the slide glass in sequence to avoid generating bubbles. Placed in a wet box and incubated at room temperature (18-25 ℃) for 30 minutes. During the incubation, liquid drying should be avoided.

Cleaning for the first time: the slides were rinsed with beaker PBS-Tween buffer running water for 1 second, taking care that the water was not too urgent and was not directed against the substrate. Then, it was immediately immersed in a cuvette containing PBS-Tween buffer for at least 5 minutes.

Incubation of secondary antibody: the slide was removed from the wash cup and edge moisture was wiped off with absorbent paper. To each reaction zone 25ul of fluorescein isothiocyanate-labeled goat anti-human IgG was added to avoid air bubbles. Incubate in a wet box at room temperature (18-25 ℃) for 30 minutes in the absence of light. During the incubation, liquid drying should be avoided.

And (3) cleaning for the second time: the slides were rinsed with beaker PBS-Tween buffer running water for 1 second, taking care that the water was not too urgent and was not directed against the substrate. Then, it was immediately immersed in a cuvette containing PBS-Tween buffer for at least 5 minutes.

Sealing piece: a capping agent (glycerin) is dripped into each reaction area of the slide, and the slide is lightly covered on the slide, so that bubbles are avoided as much as possible.

And finally, judging the result. Fluorescence was observed under a fluorescence microscope.

The existing experimental operation steps can be known to be complex in the experimental process, so that when the sample amount is large, the time interval from the first hole to the last hole for sample addition is long, the incubation time of the samples is inconsistent before and after the sample addition, the result is deviated, and the test accuracy is affected.

In addition, the following problems are often encountered when using this method:

1. when a sample is directly added on a biological slide, once the sample is misplaced, the sample cannot be returned or changed, so that the slide is wasted;

2. In order to maintain the humidity in the experimental process, an additional humidification box is needed, otherwise, the sample volatilizes due to air drying;

3. Only a single Zhang Zaipian of cleaning and soaking can be carried out, which results in slower experimental speed.

Therefore, in order to ensure the accuracy of immunofluorescence experiments and improve the experimental efficiency, it is necessary to improve the existing immunofluorescence sample adding device.

Disclosure of utility model

Aiming at the technical problems in the prior art, the application provides an indirect immunofluorescence sample adding device which is used for improving the accuracy and efficiency of immunofluorescence experiments.

In order to achieve the above object, the present application provides the following technical solutions:

An indirect immunofluorescence sample addition device comprising: the slide glass comprises a plurality of slide glass units, a slide glass support and a sample plate group, wherein the slide glass support comprises a plurality of grooves which are arranged in parallel with each other, and the slide glass units are detachably inserted and fixed on the slide glass support through the grooves; the sample adding plate group comprises a sample adding frame part and a plurality of sample adding units which are integrally arranged with the sample adding frame part and are used for placing samples to be tested, the sample adding units are rectangular, the end part of one side of the short side of each rectangle of each sample adding unit is fixed on the sample adding frame part, the end part of the other side of each rectangle of each sample adding unit is a free end, and the plurality of sample adding units are equidistantly spaced and are arranged in parallel and correspond to the grooves; the slide glass unit and the sample adding unit are correspondingly arranged on the surfaces of the slide glass support and the sample adding plate group, and are used for covering the sample to be tested at the corresponding position of the sample adding unit in the state that the slide glass support and the sample adding plate group are oppositely interleaved.

Preferably, a plurality of sample adding holes are arranged on each sample adding unit, and the samples to be tested are placed in the sample adding holes.

Preferably, a plurality of the wells are arranged in a matrix on the loading unit.

Preferably, the slide unit is provided with a transparent slide window corresponding to the sample loading hole.

Preferably, the slide support comprises a plurality of slide unit support frames and a slide frame, wherein the slide unit support frames and the slide frame are integrally formed, and two adjacent slide unit support frames and slide frames are enclosed to form the groove; wherein a plurality of the slide unit support frames are equidistantly spaced from one another.

Preferably, the long edge of the slide glass unit slides along the groove, so that the slide glass unit is detachably inserted and fixed on the slide glass bracket through the groove.

Preferably, a plurality of the slide units have a thickness less than a thickness of the slide unit support frame and the slide frame.

Preferably, the sum of the thicknesses of the slide unit and the loading unit is the same as the thicknesses of the slide unit support and the slide frame, so that the slide unit and the loading unit are engaged with each other in the up-down covering direction in a state where the slide holder and the loading plate group are opposed to each other.

Preferably, the slide holder is made of transparent resin or glass.

Preferably, the loading units are the same as the slide units in number.

The application uses the indirect immunofluorescence sample adding device to add samples through the sample adding plate, so that samples can be simultaneously incubated. When the sample adding error is found, the sample adding error area of the sample adding plate is only required to be cleaned, and the waste of the slide glass is avoided. After the slide support and the sample adding plate group are combined, a relatively closed environment is manufactured, and volatilization of a sample is effectively avoided. Meanwhile, after the two are combined, the slide glass can be turned over and switched to the positive buckle for incubation, so that the positive buckle mode slide glass substrate and the sample are incubated more fully, and the reaction effect is better. The indirect immunofluorescence sample adding device can improve the accuracy and efficiency of immunofluorescence experiments.

Drawings

Preferred embodiments of the present application will be described in further detail below with reference to the attached drawing figures, wherein:

FIG. 1 is a schematic diagram of the structure of a slide holder according to one embodiment of the application;

fig. 2 is a schematic structural view of a slide unit according to one embodiment of the application;

FIG. 3 is a schematic diagram of the structure of a set of heating plates according to one embodiment of the application;

FIG. 4 is a schematic view of the A-direction structure of FIG. 3;

FIG. 5 is a schematic view of the slide holder of the present application in a relatively interleaved state with a set of sample plates; and

Fig. 6 is a schematic diagram of the B-direction structure of fig. 5.

Reference numerals illustrate:

1. Adding a template group; 2. a slide holder; 3. a slide unit; 10. a sample adding unit; 101. a sample adding hole; 11a loading frame portion; 20. a slide unit support; 21. a slide frame; 22. a groove; 301. slide window.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

In the following detailed description, reference is made to the accompanying drawings, which form a part hereof, and in which is shown by way of illustration specific embodiments of the application. In the drawings, like reference numerals describe substantially similar components throughout the different views. Various specific embodiments of the application are described in sufficient detail below to enable those skilled in the art to practice the teachings of the application. It is to be understood that other embodiments may be utilized or structural, logical, or electrical changes may be made to embodiments of the present application.

Fig. 1 is a schematic structural view of a slide holder according to an embodiment of the present application. Fig. 2 is a schematic structural view of a slide unit according to one embodiment of the application. Fig. 3 is a schematic structural view of a set of heating plates according to an embodiment of the present application. As shown in fig. 1 and 2, in combination with fig. 3, in the present embodiment, the indirect immunofluorescence loading apparatus includes a plurality of slide units 3, a slide holder 2, and a loading plate set 1. As shown in fig. 1 and in conjunction with fig. 2, in the present embodiment, the slide holder 2 includes a plurality of grooves 22 disposed parallel to each other, and the slide unit 3 is detachably inserted and fixed to the slide holder 2 through the grooves 22 (not shown in the drawings). The slide support 2 comprises a plurality of slide unit support frames 20 and a slide frame 21, wherein the slide unit support frames 20 and the slide frame 21 are integrally formed, and two adjacent slide unit support frames 20 and the slide frame 21 are enclosed to form the groove 22; wherein a plurality of the slide unit supports 20 are disposed at equidistant intervals from one another. In operation, the slide unit 3 is slid along the recess 22 such that the slide unit 3 is removably inserted into and secured to the slide holder 2 via the recess 22. The plurality of slide units 3 have a thickness less than the thicknesses of the slide unit support frame 20 and the slide frame 21. By means of the slide holder 2, a plurality of slide units can be simultaneously washed and soaked at a time. For example, as shown in fig. 1, 5 pairs of grooves 22 are formed in the slide holder 2, and 5 slide units can be inserted at the same time, so that when cleaning or soaking is performed, the 5 slide units can be operated at the same time, which is equivalent to improving the working efficiency by 5 times compared with the original method of cleaning or soaking one slide.

Fig. 4 is a schematic view of the a-direction structure of fig. 3. As shown in fig. 3 and 4, the sample loading plate set 1 includes a sample loading frame 11 and a plurality of sample loading units 10 integrally disposed therewith for placing samples to be tested, the sample loading units 10 are rectangular, and an end portion of one side of a short side of each rectangle of the sample loading units 10 is fixed on the sample loading frame 111, and an end portion of the other side is a free end, and the plurality of sample loading units 10 are disposed in parallel and equidistantly spaced, and are disposed corresponding to the grooves 22 on the carrier unit support 20. In this embodiment, a plurality of loading wells 101 are disposed on each loading unit 10, and the sample to be measured is placed in the loading well 101. The plurality of loading wells 101 are arranged in a matrix on the loading unit 10. As shown in fig. 2, in some embodiments, the slide unit 3 is provided with a transparent slide window 301 corresponding to the loading hole 101, and the experimental progress is conveniently observed through the transparent slide window 301.

Fig. 5 is a schematic structural view of the slide holder and the sample plate set of the present application in a relatively interleaved state. Fig. 6 is a schematic diagram of the B-direction structure of fig. 5. As shown in fig. 5 and 6, in some embodiments, each of the slide units 3 is disposed corresponding to a surface shape of each of the loading units 10, so that the slide units 3 cover the sample to be measured at corresponding positions of the loading units 10 in a state that the slide holder 2 is opposite to the loading plate set 1. The loading units 10 are the same as the slide units 3 in number. After the sample adding plate group 1 is combined with the slide support 2, a relatively airtight environment is manufactured, and sample volatilization caused by air drying of a sample is effectively avoided. Therefore, it is no longer necessary to add an additional humidification cartridge in order to maintain the humidity during the experiment. Meanwhile, after the two are combined, the slide glass can be turned over and switched to the positive buckle for incubation, so that the positive buckle mode slide glass substrate and the sample are incubated more fully, and the reaction effect is better.

In some embodiments, the sum of the thicknesses of the slide unit 3 and the loading unit 10 is the same as the thicknesses of the slide unit support 20 and the slide frame 21, so that the slide unit 3 and the loading unit 10 are engaged with each other in the up-down covering direction in a state where the slide holder 2 is opposed to the loading plate group 1. In some embodiments, the slide holder 2 is made of transparent resin or glass. Meanwhile, the sample adding plate group can be made of transparent resin materials, and is low in cost and good in transmittance. Moreover, it can be made into disposable consumables. After each sample addition, the sample can be discarded as medical waste, so that the possibility of cross contamination of samples can be effectively eliminated, and the personnel cleaning cost is reduced.

The above embodiments are provided for illustrating the present application and not for limiting the present application, and various changes and modifications may be made by one skilled in the relevant art without departing from the scope of the present application, therefore, all equivalent technical solutions shall fall within the scope of the present disclosure.

Claims

1. An indirect immunofluorescence sample addition device comprising: a plurality of slide units, slide supports and sample plate groups, which is characterized in that,

The slide glass support comprises a plurality of grooves which are arranged in parallel, and the slide glass unit is detachably inserted and fixed on the slide glass support through the grooves;

The sample adding plate group comprises a sample adding frame part and a plurality of sample adding units which are integrally arranged with the sample adding frame part and are used for placing samples to be tested, the sample adding units are rectangular, the end part of one side of the short side of each rectangle of each sample adding unit is fixed on the sample adding frame part, the end part of the other side of each rectangle of each sample adding unit is a free end, and the plurality of sample adding units are equidistantly spaced and are arranged in parallel and correspond to the grooves;

The slide glass unit and the sample adding unit are correspondingly arranged on the surfaces of the slide glass support and the sample adding plate group, and are used for covering the sample to be tested at the corresponding position of the sample adding unit in the state that the slide glass support and the sample adding plate group are oppositely interleaved.

2. The indirect immunofluorescence loading device of claim 1, wherein a plurality of loading wells are disposed on each loading unit, and the sample to be tested is placed in the loading wells.

3. The indirect immunofluorescence loading device of claim 2, wherein a plurality of the loading wells are arranged in a matrix on the loading unit.

4. The indirect immunofluorescence loading device of claim 2, wherein a transparent slide window corresponding to the loading well is provided on the slide unit.

5. The indirect immunofluorescence sample loading device of claim 1, wherein the slide holder comprises a plurality of slide unit holders and a slide frame, the slide unit holders and the slide frame being integrally formed, adjacent two of the slide unit holders and the slide frame being enclosed to form the recess; wherein a plurality of the slide unit support frames are equidistantly spaced from one another.

6. The indirect immunofluorescence loading device of claim 5, wherein the long edge of the slide unit slides along the groove, such that the slide unit is removably inserted and secured to the slide holder via the groove.

7. The indirect immunofluorescence loading device of claim 5, wherein a thickness of a plurality of the slide units is less than a thickness of the slide unit support and the slide frame.

8. The indirect immunofluorescence loading device of claim 5, wherein a sum of thicknesses of the slide unit and the loading unit is the same as a thickness of the slide unit support and the slide frame such that the slide unit and the loading unit are engaged with each other in upper and lower covering directions in a state where the slide support and the loading plate group are opposed to each other.

9. The indirect immunofluorescence sample addition device of claim 1, wherein the slide holder is made of transparent resin or glass.

10. The indirect immunofluorescence loading device of claim 1, wherein the loading units are provided in the same number as the slide units.