CN207946436U - A kind of enzyme-linked immunosorbent assay device - Google Patents
A kind of enzyme-linked immunosorbent assay device Download PDFInfo
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- CN207946436U CN207946436U CN201820549735.3U CN201820549735U CN207946436U CN 207946436 U CN207946436 U CN 207946436U CN 201820549735 U CN201820549735 U CN 201820549735U CN 207946436 U CN207946436 U CN 207946436U
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- China
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- insulating box
- enzyme
- testing stand
- linked immunosorbent
- immunosorbent assay
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Abstract
The utility model discloses a kind of enzyme-linked immunosorbent assay devices, including carrier, the carrier upper surface is equipped with testing stand, and it is provided with rinse bath above testing stand, the both sides of the testing stand are fixed with column, described fixed end one end is connect by guide rod with cleaning brush, the carrier upper surface is provided with insulating box, it is provided with heat-preserving cover plate at the top of the insulating box, the insulating box front surface is respectively arranged with watch window, power switch and thermoregulation knob, the watch window is located at the top of power switch and thermoregulation knob, and power switch is located at the side of thermoregulation knob, both sides are both provided with sliding rail inside the insulating box, the sliding shoe is fixedly arranged above bearing carrier, having heaters is installed in bottom in the insulating box, and there are three heater settings.The utility model can carry out the ELISA Plate in enzyme-linked immunosorbent assay automation cleaning and sample-adding, ensure that ELISA Plate stores in the environment of constant temperature so that experimental result is more accurate.
Description
Technical field
The utility model is related to Bioexperiment equipment technical field, specially a kind of enzyme-linked immunosorbent assay device.
Background technology
Enzyme-linked immunosorbent assay is technology most widely used in enzyme immunoassay technique.Its basic skills is will be known
Antigen or antibody are adsorbed on surface of solid phase carriers, and the antigen-antibody reaction that enzyme marks is made to be carried out in solid phase surface, will with washing method
Free ingredient in liquid phase removes.Common ELISA method has double antibody sandwich method and indirect method, the former is anti-for detecting macromolecular
Original, the latter is for measuring specific antibody.The basic principle of ELISA method is enzyme molecule and antibody or antiantibody molecule covalent knot
It closes, such combination will not change the immunological characteristic of antibody, nor affect on the biological activity of enzyme.Such enzymic-labelled antibody can be with
The antigen or antibody being adsorbed on solid phase carrier are specifically bound.After substrate solution is added dropwise, substrate can make under enzyme effect
Hydrogen donor contained by it becomes coloured oxidized form by colourless reduced form, color reaction occurs.Therefore, the face of substrate can be passed through
Colour response determines whether corresponding immune response, and in the depth and sample of color reaction the amount of corresponding antibodies or antigen is in just
Than.Such chromogenic reaction can be quantitative determined by ELISA detectors, thus by the sensibility and antigen of enzymology reaction
The specific binding of antibody response is got up, and ELISA method is made to become a kind of not only special but also sensitive detection method.
Current enzyme-linked immunosorbent assay apparatus structure is complicated, and the degree of automation is relatively low, needs manually to be added
Sample, while can not ensure that ELISA Plate is stored at a constant temperature, therefore a kind of novel enzyme-linked exempt from is developed in market in urgent need
Epidemic disease adsorption test device is existing to help people to solve the problems, such as.
Utility model content
The purpose of this utility model is to provide a kind of enzyme-linked immunosorbent assay devices, to solve in above-mentioned background technology
The problem of proposition.
To achieve the above object, the utility model provides the following technical solutions：A kind of enzyme-linked immunosorbent assay device, packet
Carrier is included, the carrier upper surface is equipped with testing stand, and rinse bath is provided with above testing stand, and the two of the testing stand
Side is fixed with column, and is connected by crossbeam between column, and the beam surface upper is equipped with driving motor, and driving motor is logical
It crosses main shaft to connect with fixed end, described fixed end one end is connect by guide rod with cleaning brush, and the carrier upper surface is set
It is equipped with insulating box, and insulating box is located at the side of testing stand, is provided with heat-preserving cover plate at the top of the insulating box, and in heat-preserving cover plate
Table wall is inlaid with temperature sensor, and the insulating box front surface is respectively arranged with watch window, power switch and thermoregulation knob, institute
The top that watch window is located at power switch and thermoregulation knob is stated, and power switch is located at the side of thermoregulation knob, the constant temperature
Both sides are both provided with sliding rail inside case, and sliding rail surface is equipped with sliding shoe, and the sliding shoe is fixedly arranged above bearing carrier, and
ELISA Plate is provided between bearing carrier, having heaters is installed in bottom in the insulating box, and there are three heater settings.
Preferably, the cleaning brush is provided with two groups altogether, and cleaning brush lower surface is provided with bristle.
Preferably, the heater is connect by conducting wire with thermoregulation knob.
Preferably, sample injector is installed above the testing stand, and one end of sample injector is provided with sample-adding driver.
Preferably, the sample-adding driver bottom is connect by solution storage box with sample-adding suction nozzle.
Compared with prior art, the utility model has the beneficial effects that：The experimental provision equipped with automated cleaning equipment,
Automation scrub can be carried out to the ELISA Plate of experiment, improve the cleaning efficiency of ELISA Plate, while installation above testing stand
There is a sample injector, one end of sample injector is provided with sample-adding driver, and sample-adding driver drives sample-adding suction nozzle on testing stand to placing
ELISA Plate carries out automatic sample, and sample-adding driver promotes the reaction solution in solution storage box to enter enzyme by being loaded suction nozzle and being added dropwise
At slot on target, the problems such as being manually loaded caused germ contamination is avoided, has ensured the accurate of experimental result
Property, carrier upper surface are provided with insulating box, ELISA Plate are placed on bearing carrier, and bottom is equipped with heating in insulating box
Device, heater are connect by conducting wire with thermoregulation knob, after heater using thermoregulation knob come to insulating box internal temperature into
Row is adjusted, and ensures that ELISA Plate is stored in the steady temperature needed for experiment.
Description of the drawings
Fig. 1 is the front view of the utility model；
Fig. 2 is the insulating box internal structure schematic diagram of the utility model；
Fig. 3 is the sample injector structural schematic diagram of the utility model.
In figure：1, column；2, driving motor；3, main shaft；4, crossbeam；5, insulating box；6, watch window；7, heat-preserving cover plate；
8, fixed end；9, guide rod；10, cleaning brush；11, rinse bath；12, testing stand；13, carrier；14, power switch；15, temperature adjustment
Knob；16, sliding rail；17, bearing carrier；18, sliding shoe；19, heater；20, temperature sensor；21, ELISA Plate；22, it is loaded
Device；23, it is loaded driver；24, solution storage box；25 sample-adding suction nozzles.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, a kind of embodiment provided by the utility model：A kind of enzyme-linked immunosorbent assay device, including
Carrier 13,13 upper surface of carrier is equipped with testing stand 12, and 12 top of testing stand is provided with rinse bath 11, testing stand 12
Both sides are fixed with column 1, and are connected by crossbeam 4 between column 1, and 4 upper surface of crossbeam is equipped with driving motor 2, driving motor 2
Model 110SG-M02030, and driving motor 2 is connect by main shaft 3 with fixed end 8, driving motor 2 is driven by main shaft 3
Fixed end 8 rotates, and 8 one end of fixed end is connect by guide rod 9 with cleaning brush 10, to drive cleaning brush 10 to rinse bath 11
The ELISA Plate 21 that inside is placed carries out automation scrub, opens driving motor 2 and drives cleaning brush 10 to the enzyme mark in rinse bath 11
Plate 21 carries out automation scrub, improves the cleaning efficiency of ELISA Plate 21, and 13 upper surface of carrier is provided with insulating box 5, and permanent
Incubator 5 is located at the side of testing stand 12, and 5 top of insulating box is provided with heat-preserving cover plate 7, and 7 inner surface wall of heat-preserving cover plate is inlaid with temperature
Sensor 20,20 model pt1000 of temperature sensor are spent, temperature sensor 20 can be used for detecting 5 internal temperature of insulating box,
5 front surface of insulating box is respectively arranged with watch window 6, power switch 14 and thermoregulation knob 15, and watch window 6 is located at power switch
14 and thermoregulation knob 15 top, and power switch 14 is located at the side of thermoregulation knob 15, and 5 inside both sides of insulating box are both provided with
Sliding rail 16, and 16 surface of sliding rail is equipped with sliding shoe 18, sliding shoe 18 are fixedly arranged above bearing carrier 17, and bearing carrier 17 it
Between be provided with ELISA Plate 21, sliding shoe 18 can drive the vertical sliding motion on sliding rail 16 of bearing carrier 17, convenient to ELISA Plate 21
It is fetched, having heaters 19 is installed in bottom in insulating box 5, and there are three the settings of heater 19, cleaning brush 10 is provided with two altogether
Group, and 10 lower surface of cleaning brush is provided with bristle, heater 19 is connect by conducting wire with thermoregulation knob 15, after heater 19
55 internal temperature of insulating box is adjusted using thermoregulation knob 15, ensures ELISA Plate 21 in the steady temperature needed for experiment
It is stored, 12 top of testing stand is equipped with sample injector 22, and one end of sample injector 22 is provided with sample-adding driver 23, and sample-adding drives
23 model D2212-980KV of dynamic device, sample-adding 23 bottom of driver are connect with sample-adding suction nozzle 25 by solution storage box 24, are loaded
Driver 23 promotes the reaction solution in solution storage box 24 to enter at the slot on ELISA Plate 21 by being loaded suction nozzle 25 and being added dropwise,
The problems such as avoiding germ contamination caused by being manually loaded.
Operation principle：In use, 13 upper surface of carrier is equipped with testing stand 12, and 12 top of testing stand is provided with cleaning
ELISA Plate 21 is put into inside rinse bath 11 by slot 11, and 4 upper surface of crossbeam is equipped with driving motor 2, and driving motor 2 passes through
Main shaft 3 is connect with fixed end 8, and 8 one end of fixed end is connect by guide rod 9 with cleaning brush 10, is opened driving motor 2 and is driven clearly
The ELISA Plate 21 scrubbed in 10 pairs of rinse baths 11 carries out automation scrub, improves the cleaning efficiency of ELISA Plate 21, testing stand 12
Top is equipped with sample injector 22, and one end of sample injector 22 is provided with sample-adding driver 23, and 23 bottom of sample-adding driver passes through solution
Storage Box 24 connect with sample-adding suction nozzle 25, and sample-adding driver 23, which drives, is loaded suction nozzle 25 to the ELISA Plate 21 placed on testing stand 12
Automatic sample is carried out, sample-adding driver 23 promotes the reaction solution in solution storage box 24 to enter enzyme by being loaded suction nozzle 25 and being added dropwise
At slot on target 21, the problems such as being manually loaded caused germ contamination is avoided, has ensured the standard of experimental result
True property, 13 upper surface of carrier are provided with insulating box 5, and 5 inside both sides of insulating box are both provided with sliding rail 16, the installation of 16 surface of sliding rail
There are sliding shoe 18, sliding shoe 18 to be fixedly arranged above bearing carrier 17, ELISA Plate 21, ELISA Plate 21 are provided between bearing carrier 17
It is placed on bearing carrier 17, sliding shoe 18 can drive the vertical sliding motion on sliding rail 16 of bearing carrier 17, convenient to enzyme mark
Plate 21 is fetched, and having heaters 19 is installed in bottom in insulating box 5, and 5 front surface of insulating box is respectively arranged with watch window 6, electricity
Source switch 14 and thermoregulation knob 15, heater 19 are connect by conducting wire with thermoregulation knob 15, and temperature adjustment is utilized after heater 19
Knob 15 is adjusted 55 internal temperature of insulating box, ensures that ELISA Plate 21 is stored up in the steady temperature needed for experiment
It deposits, the utility model can carry out the ELISA Plate 21 in enzyme-linked immunosorbent assay automation cleaning and sample-adding, ensure enzyme mark
Plate 21 stores in the environment of constant temperature so that experimental result is more accurate.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this practicality is new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing in the equivalent requirements of the claims will be fallen
All changes in justice and range are embraced therein.Any reference numeral in claim should not be considered as limitation
Involved claim.
Claims (5)
1. a kind of enzyme-linked immunosorbent assay device, including carrier (13), which is characterized in that carrier (13) upper surface
Testing stand (12) is installed, and rinse bath (11) is provided with above testing stand (12), the both sides of the testing stand (12) are fixed with
Column (1), and connected by crossbeam (4) between column (1), crossbeam (4) upper surface is equipped with driving motor (2), and drives
Dynamic motor (2) is connect by main shaft (3) with fixed end (8), and described fixed end (8) one end passes through guide rod (9) and cleaning brush
(10) it connecting, carrier (13) upper surface is provided with insulating box (5), and insulating box (5) is located at the side of testing stand (12),
It is provided with heat-preserving cover plate (7) at the top of the insulating box (5), and heat-preserving cover plate (7) inner surface wall is inlaid with temperature sensor (20), institute
It states insulating box (5) front surface and is respectively arranged with watch window (6), power switch (14) and thermoregulation knob (15), the observation window
Mouth (6) is located at the top of power switch (14) and thermoregulation knob (15), and power switch (14) is located at the one of thermoregulation knob (15)
Side, the internal both sides of the insulating box (5) are both provided with sliding rail (16), and sliding rail (16) surface is equipped with sliding shoe (18), described
Sliding shoe (18) is fixedly arranged above bearing carrier (17), and ELISA Plate (21), the constant temperature are provided between bearing carrier (17)
The interior bottom installation having heaters (19) of case (5), and there are three heater (19) settings.
2. a kind of enzyme-linked immunosorbent assay device according to claim 1, it is characterised in that：The cleaning brush (10) is altogether
Two groups are provided with, and cleaning brush (10) lower surface is provided with bristle.
3. a kind of enzyme-linked immunosorbent assay device according to claim 1, it is characterised in that：The heater (19) is logical
Conducting wire is crossed to connect with thermoregulation knob (15).
4. a kind of enzyme-linked immunosorbent assay device according to claim 1, it is characterised in that：On the testing stand (12)
Side is equipped with sample injector (22), and one end of sample injector (22) is provided with sample-adding driver (23).
5. a kind of enzyme-linked immunosorbent assay device according to claim 4, it is characterised in that：The sample-adding driver
(23) bottom is connect by solution storage box (24) with sample-adding suction nozzle (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820549735.3U CN207946436U (en) | 2018-04-04 | 2018-04-04 | A kind of enzyme-linked immunosorbent assay device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820549735.3U CN207946436U (en) | 2018-04-04 | 2018-04-04 | A kind of enzyme-linked immunosorbent assay device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207946436U true CN207946436U (en) | 2018-10-09 |
Family
ID=63696015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820549735.3U Expired - Fee Related CN207946436U (en) | 2018-04-04 | 2018-04-04 | A kind of enzyme-linked immunosorbent assay device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207946436U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117706087A (en) * | 2024-02-01 | 2024-03-15 | 山东益生种畜禽股份有限公司 | Portable detection device for bursa mycoplasma MS-ONE |
-
2018
- 2018-04-04 CN CN201820549735.3U patent/CN207946436U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117706087A (en) * | 2024-02-01 | 2024-03-15 | 山东益生种畜禽股份有限公司 | Portable detection device for bursa mycoplasma MS-ONE |
| CN117706087B (en) * | 2024-02-01 | 2024-04-16 | 山东益生种畜禽股份有限公司 | Portable detection device for bursa mycoplasma MS-ONE |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181009 Termination date: 20190404 |