TWI749558B - Cell sample centrifuge container - Google Patents

Abstract

A centrifugal container for cell specimens is composed of a cup holder and a bottom plate. Among them, the cup holder includes a base and a collecting part. The base has a downwardly curved bearing surface. The center of the bearing surface is provided with a pressing sheet. Along the periphery of the pressing sheet, there are multiple cut grooves that can make the pressing sheet elastically bend. The bottom end of the collecting part A conduit is provided, and the conduit is connected to the compressed tablet. At the same time, the two sides of the base and the bottom plate are provided with buckle parts and buckles that can be buckled with each other to combine the cup holder and the bottom plate. During the buckling process, the nozzle of the collecting part will be subjected to force. It protrudes and fits closely on the bottom plate, thereby improving the tightness of the cup holder and the bottom plate.

Description

Cell sample centrifuge container

The invention belongs to the technical field of medical bioassays, and particularly relates to a centrifugal container for cell specimens for laboratory analysis.

In medical biological research, centrifugal technology is often used to collect cell samples in order to efficiently make cell smears. During centrifugation, centrifugal force is used to separate the cells from the sediment and throw them onto the glass slide, and the centrifugal force will flatten the cell structure, so that the nucleus and nucleoli can be observed more clearly, and the slides that need to be observed can also be removed. , Use the corresponding dyes for dyeing.

The conventional centrifugal smearing device is equipped with a centrifugal smearing specimen container holder for holding the container, the sealing member, and the glass slide containing the liquid specimen. Chinese Patent No. 110799825 discloses a specimen container holder that is convenient to operate and easy to assemble. It has a base for accommodating specimen containers, seals, and glass slides in a stacked state, and uses a holder with a handle. , It can be rotated and clamped on the base in a rotating manner, which can prevent a gap between the specimen container, the sealing member, and the glass slide, which may cause the liquid specimen to leak to the outside. However, in order to achieve a tight fixation of the specimen container and the seal, the structure of the specimen container holder is easily broken under long-term clamping action.

Therefore, there is still room for improvement and development in the above-mentioned prior art.

In view of this, the present invention addresses the deficiencies in the prior art, and its main purpose is to provide a centrifugal container for cell specimens, which uses a snap structure to combine the cup holder and the bottom plate, and the base surface of the cup holder is provided with an elastically bendable pressing sheet , The duct of the collecting part is connected to the pressed sheet, which can be closely attached to the bottom plate due to the protruding force during the buckling process, so that the tightness of the cup holder and the bottom plate is greatly improved.

To achieve the above objective, the present invention provides a centrifuge container for cell specimens, which comprises a cup holder and a bottom plate. The cup holder includes a base and a collecting part. The base has a downwardly curved bearing surface. A pressing piece is arranged in the center of the bearing surface. A plurality of cut grooves are arranged along the periphery of the pressing piece to make the pressing piece elastically bend. The bottom end of the collecting part There is a conduit connected with the opening of the pressing sheet, and a buckle part is respectively provided on both sides of the base. Both sides of the bottom plate are respectively provided with a buckle, the buckle is used to press force and buckle with the buckle part to connect the cup holder to the bottom plate, and the pipe orifice of the collecting part will be affected by the force. Protruding from the opening and tightly attached to the bottom plate.

In an embodiment of the present invention, the collecting part has a conical cavity, and one end of the conical cavity is connected to the pipe; in another embodiment of the present invention, the inside of the conical cavity is divided into a first The cavity and a second cavity, the first cavity and the second cavity are respectively communicated with the two conduits and are respectively connected to the two openings of the pressing sheet.

Compared with the prior art, the present invention is provided with buckle parts and buckles that can be buckled with each other on both sides of the base part and the bottom plate to increase the convenience of operation. Furthermore, because the pipe orifice of the collecting part will be buckled It is automatically attached to the bottom plate during the closing process, which not only achieves tight fixation, effectively avoids external leakage during centrifugal operation, but also allows users to buckle in a more labor-saving manner, which greatly reduces the difficulty of overall structure design.

The following detailed descriptions are given through specific embodiments, so that it will be easier to understand the purpose, technical content, features, and effects of the present invention.

Please refer to FIG. 1, which illustrates a three-dimensional exploded schematic diagram of the cell specimen centrifuge container 100 provided by the first embodiment of the present invention.

In this embodiment, the cell specimen centrifuge container 100 includes a cup holder 10 and a bottom plate 20. The cup holder 10 and the bottom plate 20 can preferably be made of plastic materials, such as polyimide (PI) and polyether ether ketone (PEEK). , Polyamide-imide (PAI), polybenzimidazole (PBI), polyphenylene sulfide (PPS), polytetrafluoroethylene (PFA), liquid crystal polymer and other chemical-resistant resins for storage A glass slide 30 is placed between the cup holder 10 and the bottom plate 20 to facilitate the production of cell smears for detecting cell specimens. In this embodiment, a filter paper 40 may be further disposed above the glass slide 30 for solid-liquid separation.

Among them, the cup holder 10 is mainly composed of a base 11 and a collecting portion 12. The base 11 has a carrying surface 111 that is slightly downwardly curved. A pressing sheet 112 is arranged in the center of the carrying surface 111, and the pressing sheet 112 corresponds to the position of the glass slide 30. There is an opening 113 (see Fig. 4). Along the periphery of the pressed sheet 112, there are multiple cut grooves 114 that can bend the pressed sheet 112 elastically. A card is provided on both sides of the base 11 (the long side in the picture). For the buckle 115, a buckle block 116 is provided on the other side of the base 11 (the short side in the figure). The collection part 12 has a cone-shaped cavity 121, the top end of the cone-shaped cavity 121 can be used for dripping the sample solution, the bottom end of the cone-shaped cavity 121 is connected with a tube 122, and the tube 122 is connected with the opening 113 of the pressing sheet 112 .

In addition, the two sides of the bottom plate 20 (the long side in the figure) are respectively provided with a buckle 215 corresponding to the position of the buckle portion 115, which can be pressed to buckle with the buckle portion 115, and on the other side of the bottom plate 21 One side (the short side in the figure) is provided with a buckle groove 216 corresponding to the position of the buckle block 116, so that the buckle block 116 can be inserted into the buckle groove 216 to be fixed.

Next, please refer to FIGS. 2 to 5 to describe in detail the operation process and principle of the cell specimen centrifuge container 100 provided by the first embodiment of the present invention with a standard body fluid cell smear production process.

First, as shown in Figure 2, stack the clean glass slide 30 and the filter paper 40 on the surface of the bottom plate 20 in sequence, and place the cup holder 10 on the buckle block 116 on the short side of the base 11 (see No. 1 Figure) Snap into the buckle groove 216 on the short side of the bottom plate 20 (see Figure 1), so that the bottom plate 20 and the cup holder 10 are fixed on one side first.

Then, as shown in Figure 3, simultaneously press on both sides of the short side of the base 11 of the cup holder 10 to buckle the buckle portion 115 of the base 11 into the buckle 215 of the bottom plate 20 to fix the cup holder 10 And the bottom plate 20 can be combined together.

As shown in Figure 4, due to the force applied during the fastening process, the nozzle of the pipe 122 of the collecting part 12 of the cup holder 10 will protrude from the opening and abut against the bottom plate 20 due to the force, so that the cup holder 10 The glass slide 30 and the filter paper 40 sandwiched between the bottom plate 20 and the bottom plate 20 can be kept in close contact with each other, and the generation of gaps can be reduced. Then, the plastic straw 50 can be used to suck 3 to 5 drops of the sample solution derived from the body fluid into the conical cavity 121 of the collecting part 12.

Finally, as shown in Fig. 5, the cell specimen centrifuge container 100 is placed in the centrifuge 60 and centrifuged at a low speed of 800 rpm for 3 to 5 minutes. Under the action of centrifugation, the sample solution will flow along the conduit 122 through the conical cavity 121 of the collecting part 12, and then flow out through the opening 113 of the pressing sheet 112. The liquid component of the sample solution will penetrate into the filter paper 40, and the cells will be suspended. Will be smeared on the glass slide 30 (see Figure 4), and the body fluid cell smear is successfully prepared. After the smear is taken out, staining, disinfection and other procedures can be continued to facilitate observation of the cells with a microscope.

In addition, please refer to FIG. 6, which is a schematic cross-sectional structure diagram of the cell specimen centrifuge container 300 provided by the second embodiment of the present invention.

The difference from the first embodiment is that in the cup holder 310 of the cell specimen centrifuge container 300 of this embodiment, the inside of the conical cavity of the collecting portion 311 is divided into a first cavity 312 and a second cavity 313 , The first cavity 312 and the second cavity 313 are respectively communicated with the two conduits 314 and 315 and connected to the two openings 317 and 318 of the pressing piece 316 respectively. In other words, the first embodiment is a single-cavity design, and the second embodiment is a dual-cavity design. The dual-cavity design can drop two different sample solutions respectively, thereby increasing the inspection efficiency and productivity. And reduce the cost of inspection.

In summary, the centrifugal container for cell specimens provided by the present invention is composed of a cup holder and a bottom plate, and is combined with a snap structure. The structure is simple, and the assembly is easy. In the assembly process, due to the collection The tube nozzle of the lower part will automatically close to the bottom plate, so that the cup holder and the bottom plate can achieve a close combination effect, which can effectively avoid the situation of external leakage during centrifugal operation. In addition, the user of the present invention will be able to buckle in a more labor-saving manner, and there is no need to worry about insufficient structural strength of the container or designing a large-curved bottom plate for tightness requirements. Therefore, the difficulty of the overall structure design can be greatly reduced, and the overall structure design can be greatly reduced. Enhance product value and industrial competitiveness.

The above are only specific implementations of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily think of various changes or substitutions within the technical scope disclosed by the present invention. All of these should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claim.

100... Centrifuge container for cell sample 10...Cup holder 11...base 111...bearing surface 112... pressed film 113...open 114...grooving 115...Snap part 116...Snap block 12...Collection Department 121...cone cavity 122...Conduit 20...Bottom plate 215... Fasteners 216...Snap slot 30...slide 40...filter paper 50...Plastic straw 60... Centrifuge 300...Cell sample centrifuge container 310...cup holder 311...Collection Department 312...First cavity 313...Second cavity 314...Catheter 315...Catheter 316...Pressed Tablet 317...open 318...open

Figure 1 is a three-dimensional exploded schematic diagram of the centrifugal container for cell specimens provided by the first embodiment of the present invention. Figures 2 to 5 are sequential operations of the centrifugal container for cell specimens provided by the first embodiment of the present invention. Fig. 6 is a schematic cross-sectional structure diagram of the centrifugal container for cell specimens provided by the second embodiment of the present invention.

100... Centrifuge container for cell sample 10...Cup holder 11...base 111...bearing surface 112... pressed film 114...grooving 115...Snap part 116...Snap block 12...Collection Department 121...cone cavity 122...Conduit 20...Bottom plate 215... Fasteners 216...Snap slot 30...slide 40...filter paper

Claims (6)

A centrifugal container for cell specimens, comprising: a cup holder, comprising a base and a collecting part, the base has a downwardly curved carrying surface, the center of the carrying surface is provided with a pressing sheet, and the periphery of the pressing sheet is arranged to enable the A plurality of cut grooves with elastic bending of the pressed sheet, the collecting part is connected to the pressed sheet, the top of the collecting part can be used for dripping a sample solution, and the bottom end of the collecting part is provided with a conduit for dripping the sample solution into the collecting part Then it can flow along the conduit, the conduit is connected to an opening of the pressed sheet, and two sides of the base are respectively provided with a buckle part; A buckle is provided. The buckle is used for pressing and buckling with the buckle, so as to combine the cup holder with the bottom plate, so that the glass slide is clamped between the cup holder and the bottom plate. Between the bottom plates and the nozzle of the conduit of the collecting part is pressed against the glass slide by protruding from the opening. The cell specimen centrifugal container according to claim 1, wherein the collecting part has a conical cavity, and one end of the conical cavity is connected to the catheter. The cell specimen centrifuge container according to claim 2, wherein the inside of the conical cavity is divided into a first cavity and a second cavity, and the first cavity and the second cavity are respectively The conduits are conducted and respectively connected to the two openings of the pressing sheet. The cell specimen centrifuge container according to claim 1, further comprising a filter paper, and the filter paper is accommodated between the glass slide and the cup holder. The cell specimen centrifuge container according to claim 1, wherein one side of the bottom plate further includes a buckle groove, and the base further has a buckle block for inserting and fixing in the buckle groove. The cell specimen centrifuge container according to claim 1, wherein the cup holder and the bottom plate are made of plastic materials.

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