TW202144758A - Centrifugal container for cell specimen being composed of a cup base and a bottom plate and capable of improving the tightness - Google Patents

Abstract

Disclosed is a centrifugal container for a cell specimen. The centrifugal container is composed of a cup holder and a bottom plate. The cup holder includes a base and a collecting part; the base is provided with a downwards bent bearing surface, the center of the bearing surface is provided with a compressed tablet, and a plurality of cutting grooves capable of elastically bending the compressed tablet being formed along the periphery of the compressed tablet; and the bottom of the collecting part is provided with a guide pipe communicating with the compressed tablet. Meanwhile, both sides of the base and the bottom plate are provided with buckling parts and buckling members which may fasten the cup holder and the bottom plate,; and during the buckling process, a pipe orifice of the guide pipe of the collecting part may be tightly fitted to the bottom plate due to protrusion caused by stress, so that the tightness of the cup holder and the bottom plate can be improved.

Description

Cell sample centrifuge container

The invention belongs to the technical field of medical biological assays, and in particular relates to a centrifuge container for cell samples for assay analysis.

In medical biological research, cell specimens are often collected by centrifugation for efficient production of cell smears. During the centrifugation process, the cells are separated from the sediment by centrifugal force and thrown onto the glass slide, and the centrifugal force will flatten the cell structure, so that the nucleus and nucleolus can be observed more clearly, and the glass slide that needs to be observed can also be observed. , use the corresponding dye for dyeing.

The conventional centrifugal smearing device is equipped with a centrifugal smearing specimen container holder for holding the container containing the liquid specimen, the sealing member, and the glass slide. Chinese Patent No. 110799825 discloses a specimen container holder which is easy to operate and assemble, which has a base for accommodating specimen containers, sealing members and glass slides in a stacked state, and utilizes a holding portion with a handle , which can be rotated and clamped on the base, and can prevent the liquid sample from leaking to the outside due to a gap between the sample container, the seal and the glass slide. However, in order to achieve a tight fixation between the specimen container and the sealing member, the structural design of the specimen container holder is easily broken under long-term clamping action.

Therefore, the above-mentioned prior art still has room for improvement and development.

In view of this, the present invention aims at the deficiencies in the prior art, and its main purpose is to provide a centrifuge container for cell samples, which utilizes a snap-fit structure to combine a cup holder and a bottom plate, and is provided with an elastically bendable pressed sheet on the base surface of the cup holder. , the conduit of the collecting part is connected to the pressed sheet, which can be closely attached to the bottom plate due to the protruding force during the buckling process, so that the tightness between the cup holder and the bottom plate is greatly improved.

In order to achieve the above object, the present invention provides a cell sample centrifuge container, which includes a cup holder and a bottom plate. Wherein, the cup holder includes a base and a collection portion, the base has a downwardly curved bearing surface, a pressing sheet is arranged in the center of the bearing surface, and along the periphery of the pressing sheet is a plurality of slits that can elastically bend the pressing sheet, and the bottom end of the collecting portion There is a conduit, the conduit is communicated with the opening of the pressed tablet, and two sides of the base portion are respectively provided with a snap portion. The two sides of the bottom plate are respectively provided with a buckle piece. The buckle piece is used to press the force and engage with the buckle part to connect the cup holder to the bottom plate. It protrudes from the opening and fits snugly on the bottom plate.

In an embodiment of the present invention, the collecting portion has a conical cavity, and one end of the conical cavity is connected to a conduit; in another embodiment of the present invention, the inside of the conical cavity is divided into a first The cavity and a second cavity, the first cavity and the second cavity are respectively communicated with the two conduits and are respectively connected to the two openings of the pressed tablet.

Compared with the prior art, the present invention is provided with a buckle portion and a buckle member that can be buckled with each other on both sides of the base portion and the bottom plate, so as to increase the convenience of operation. Furthermore, since the conduit opening of the collecting portion will be buckled in the During the closing process, it is automatically attached to the bottom plate, which can not only achieve a tight fixation and effectively avoid external leakage during centrifugation, but also allow the user to perform the fastening in a more labor-saving way, which also greatly reduces the difficulty of the overall structural design.

The following describes in detail with specific embodiments, when it is easier to understand the purpose, technical content, characteristics and effects of the present invention.

Please refer to FIG. 1 , which shows a schematic exploded perspective view of the centrifuge container 100 for cell samples provided by the first embodiment of the present invention.

In this embodiment, the cell sample centrifuge container 100 includes a cup holder 10 and a bottom plate 20 . The cup holder 10 and the bottom plate 20 are preferably made of plastic materials, such as polyimide (PI), polyetheretherketone (PEEK) , polyamide-imide (PAI), polybenzimidazole (PBI), polyphenylene sulfide (PPS), polytetrafluoroethylene (PFA), liquid crystal polymer and other chemical-resistant resins to accommodate A glass slide 30 is placed between the cup holder 10 and the bottom plate 20 to facilitate making a cell smear for detecting cell samples. In this embodiment, a filter paper 40 may be further disposed above the glass slide 30 for solid-liquid separation.

The cup holder 10 is mainly composed of a base portion 11 and a collection portion 12 . The base portion 11 has a slightly curved bearing surface 111 , and a pressing sheet 112 is arranged in the center of the bearing surface 111 , and the pressing sheet 112 corresponds to the position of the glass slide 30 . There is an opening 113 (see Fig. 4), along the periphery of the pressed sheet 112 are multiple slots 114 that can elastically bend the pressed sheet 112, and two sides of the base 11 (long sides in the figure) are respectively provided with a card In the buckle portion 115 , a buckle block 116 is provided on the other side (the short side in the figure) of the base portion 11 . The collection part 12 has a conical cavity 121 , the top of the conical cavity 121 can be dripped into the sample solution, the bottom end of the conical cavity 121 is connected with a conduit 122 , and the conduit 122 is connected with the opening 113 of the pressed tablet 112 .

In addition, two sides of the bottom plate 20 (the long side in the figure) are respectively provided with a latching member 215 at the position corresponding to the above-mentioned latching portion 115 , which can be pressed by force to engage with the latching portion 115 . One side (the short side in the figure) is provided with a snap groove 216 corresponding to the position of the snap block 116 , so that the snap block 116 can be inserted into the snap slot 216 for fixing.

Next, referring to FIG. 2 to FIG. 5 , the operation process and principle of the cell sample centrifuge container 100 provided by the first embodiment of the present invention will be described in detail by using a standard production process of body fluid cell smear.

First, as shown in Fig. 2, the cleaned glass slide 30 and the filter paper 40 are sequentially stacked on the surface of the bottom plate 20, and the cup holder 10 is connected to the snap block 116 on the short side of the base 11 (see Section 1 Figure) snap into the snap groove 216 on one side of the short side of the bottom plate 20 (see Figure 1), so that the bottom plate 20 and the cup holder 10 are fixed on one side first.

Then, as shown in FIG. 3 , press the two sides of the short side of the base 11 of the cup holder 10 with force at the same time, and fasten the buckle portion 115 of the base 11 into the buckle member 215 of the bottom plate 20 to fix it, so that the cup holder 10 is fixed. and the bottom plate 20 can be combined together.

As shown in FIG. 4 , during the buckling process, due to the force applied, the nozzle of the conduit 122 of the collecting portion 12 of the cup holder 10 will be pressed against the direction of the bottom plate 20 due to the protruding opening of the cup holder 10 , so that the cup holder 10 The bottom plate 20 and the glass slide 30 and the filter paper 40 sandwiched therebetween can be kept in close contact with each other, thereby reducing the occurrence of gaps. Then, the plastic pipette 50 can be used to suck 3-5 drops of the sample solution derived from the body fluid into the conical cavity 121 of the collecting part 12 .

Finally, as shown in FIG. 5 , the cell sample centrifuge container 100 is placed in the centrifuge 60 and centrifuged at a low speed of 800 rpm for 3 to 5 minutes. Under the centrifugal action, the sample solution will flow along the conduit 122 through the conical cavity 121 of the collecting part 12, and then flow out through the opening 113 of the pressing sheet 112, and the liquid components of the sample solution will penetrate into the filter paper 40, which will suspend the cells will be smeared onto slide 30 (see Figure 4) and a humoral cell smear is successfully made. After removing the smear, staining, disinfection and other procedures can be continued to facilitate the observation of cells with a microscope.

In addition, please refer to FIG. 6 , which is a schematic cross-sectional structure diagram of the cell sample centrifuge container 300 provided by the second embodiment of the present invention.

Different from the first embodiment, in the cup holder 310 of the cell sample centrifuge container 300 in this embodiment, the inside of the conical cavity of the collecting portion 311 is divided into a first cavity 312 and a second cavity 313 . , the first cavity 312 and the second cavity 313 communicate with the two conduits 314 and 315 respectively and are respectively connected to the two openings 317 and 318 of the pressing sheet 316 . That is to say, the first embodiment is a single-chamber design, and the second embodiment is a dual-chamber design. The dual-chamber design can respectively drop two different sample solutions, thereby increasing the inspection efficiency and productivity. reduce inspection costs.

To sum up, the cell sample centrifuge container provided according to the present invention is composed of two parts, a cup holder and a bottom plate, and is combined by a snap-fit structure, which has a simple structure and is easy to assemble. The tube orifice of the tube at the bottom will automatically close to the bottom plate, so that the cup holder and the bottom plate can be tightly combined, which can effectively avoid external leakage of the test during centrifugation. In addition, the user of the present invention will be able to fasten in a more labor-saving way, and do not have to worry about the insufficient structural strength of the container or design a large-curved bottom plate for the tightness requirement, so the difficulty of the overall structure design can be greatly reduced, and the Improve product value and industrial competitiveness.

The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited to this. Any person skilled in the art can easily think of various changes or replacements thereof within the technical scope disclosed by the present invention. , these should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

100: Cell sample centrifuge container 10: Cup Holder 11: Base 111: Bearing surface 112: Pressed Tablets 113: Opening 114: Grooving 115: Buckle part 116: Snap block 12: Collection Department 121: Conical cavity 122: Catheter 20: Bottom plate 215: Snaps 216: Buckle slot 30: slides 40: filter paper 50: Plastic straws 60: Centrifuge 300: Cell sample centrifuge container 310: Cup Holder 311: Collection Department 312: The first cavity 313: Second cavity 314: Catheter 315: Catheter 316: Pressed Tablets 317: Opening 318: Opening

FIG. 1 is a schematic exploded perspective view of the centrifuge container for cell samples provided by the first embodiment of the present invention. FIG. 2 to FIG. 5 sequentially illustrate the operation process of the cell sample centrifuge container provided by the first embodiment of the present invention. FIG. 6 is a schematic cross-sectional structure diagram of a cell sample centrifuge container provided by a second embodiment of the present invention.

100: Cell sample centrifuge container

10: Cup Holder

11: Base

111: Bearing surface

112: Pressed Tablets

114: Grooving

115: Buckle part

116: Snap block

12: Collection Department

121: Conical cavity

122: Catheter

20: Bottom plate

215: Snaps

216: Buckle slot

30: slides

40: filter paper

Claims (7)

A cell sample centrifuge container, comprising: A cup holder, comprising a base and a collection portion, the base has a downwardly curved bearing surface, the center of the bearing surface is provided with a pressed sheet, and along the periphery of the pressed sheet, a plurality of cutting grooves are arranged to enable the pressed sheet to bend elastically, The bottom end of the collecting portion has a conduit, the conduit communicates with an opening of the pressed tablet, and two sides of the base are respectively provided with a snap portion; and A bottom plate, two sides of which are respectively provided with a buckling piece, the buckling piece is used for pressing with force to be engaged with the buckling portion, so as to combine the cup holder on the bottom plate, and the collecting portion is The orifice of the conduit sticks against the bottom plate due to the force protruding from the opening. The cell sample centrifuge container according to claim 1, wherein the collecting part has a conical cavity, and one end of the conical cavity is connected to the conduit. The cell sample centrifuge container according to claim 2, wherein the inside of the conical cavity is divided into a first cavity and a second cavity, and the first cavity and the second cavity are respectively connected with the two The conduits conduct and are respectively connected to the two openings of the pressed tablet. The cell sample centrifuge container according to claim 1, further comprising a glass slide, and the glass slide is accommodated between the cup holder and the bottom plate. The cell sample centrifuge container according to claim 4, further comprising a filter paper, the filter paper is accommodated between the glass slide and the cup holder. The cell sample centrifuge container according to claim 1, wherein one side of the bottom plate further comprises a snap groove, and the base further has a snap block for being inserted into the snap groove and fixed. The cell sample centrifuge container according to claim 1, wherein the cup holder and the bottom plate are made of plastic materials.

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