CN219334254U

CN219334254U - Trace high-volatility liquid sample adding device

Info

Publication number: CN219334254U
Application number: CN202320583831.0U
Authority: CN
Inventors: 张停; 陈海洋; 周宋兵
Original assignee: Hangzhou Desinpro Biotechnology Co ltd
Current assignee: Hangzhou Desinpro Biotechnology Co ltd
Priority date: 2023-03-23
Filing date: 2023-03-23
Publication date: 2023-07-14
Anticipated expiration: 2033-03-23

Abstract

The utility model provides a trace high-volatility liquid sample adding device which comprises a device frame, a sample adding unit, a needle washing unit and a plurality of soft rubber tubes, wherein the sample adding unit comprises a plunger pump, a sample adding needle and a needle mounting seat, the plunger pump is arranged at one end of the device frame, the plunger pump is fixedly connected with the device frame, the sample adding needle and the needle mounting seat are in sliding fit, the sample adding needle is fixed at the top of the needle mounting seat by a connector, a connecting pipe is arranged at the bottom of the connector, a sealing piece fixed by the sample adding needle is sleeved on the outer wall of the connecting pipe and can slide up and down, the connector and the sealing piece are connected by a spring, so that the sample adding needle floats up and down corresponding to the needle mounting seat, collision with a user is avoided, the tip of the sample adding needle is ensured to be clung to the surface of a slide at the same time, the mixed liquid is ensured to be absorbed by the surface tension of the slide, the plunger pump is communicated with the sample adding needle, and the liquid level detection conducting piece is matched with each other, and thus the liquid absorption and the ejection of the sample adding needle are accurately controlled.

Description

Trace high-volatility liquid sample adding device

Technical Field

The utility model relates to a sample adding device, in particular to a trace high-volatility liquid sample adding device, and belongs to the technical field of liquid sample adding.

Background

Generally, 2ul of mixed liquid containing volatile cells is dripped on a glass slide manually by using a sample-adding needle, and a stable cell-residual cell layer is formed after volatilization is finished and is used for staining microscopic observation.

Because volatile liquid can volatilize soon in open environment, lead to liquid absorption unstable in the absorption process, can produce behind the liquid level and hang the drip at the application of sample needle point, increase volatilize the area and accelerate volatilize and cause follow-up dropwise add the mixed liquid volume too little, the cell circle stacks up, simultaneously because the liquid addition volume is less, can adhere to on the application of sample needle point because of liquid tension reason can not get off.

Therefore, a solution for dropping a trace amount of a highly volatile liquid is required.

Disclosure of Invention

Based on the background, the utility model aims to provide a trace high-volatility liquid sample adding device capable of transferring and adding samples to trace liquid with high precision and stably dripping the samples onto a glass sheet, and solves the problems in the background technology.

In order to achieve the above object, the present utility model provides the following technical solutions:

the utility model provides a trace high volatile liquid sample loading device, includes device frame, application of sample unit, washes needle unit and a plurality of soft rubber tube, the application of sample unit includes plunger pump, superfine needle diameter's application of sample needle and needle mount pad, the plunger pump sets up in device frame one end, plunger pump and device frame fixed connection, the plunger pump passes through soft rubber tube with the needle mount pad and is connected, the application of sample needle sets up inside the needle mount pad, application of sample needle and needle mount pad sliding fit, be fixed with the liquid level between needle mount pad and the application of sample needle and survey the conducting strip, the liquid level surveys the conducting strip and is connected with the plunger pump electricity, the needle mount pad is provided with joint, connecting pipe and spring, the corresponding application of sample needle of joint sets up at needle mount pad top, needle mount pad and joint fixed connection, the joint bottom is provided with the connecting pipe, connecting pipe and joint fixed connection, the application of sample needle cup joints in the outside, application of sample needle and connecting pipe sliding fit, the application of sample needle outside is provided with the sealing member, sealing member and application of sample needle fixed connection, sealing member and sliding fit are provided with sealing member and spring, sealing member and spring. The setting device frame can fix partial sample adding unit and needle washing unit, the sample adding needle with superfine needle diameter can reduce surface tension, reduce liquid volatilization surface area, the plunger pump provides power for the sample adding unit, the sample adding needle, the connecting pipe, the connector and the plunger pump are communicated in series, thereby the plunger pump can control the sample adding needle to absorb liquid, as the connector is fixed at the top of the needle mounting seat, the sealing element and the sample adding needle are fixed and are sleeved on the outer wall of the connecting pipe, after the spring is arranged between the connector and the sealing element, the sample adding needle can float up and down, collision with a user is avoided, the tip of the sample adding needle is tightly attached to the surface of a slide, and the mixed liquid is ensured to be absorbed by the surface tension of the slide completely.

Preferably, the needle washing unit comprises a needle washing pool, a water storage tank, an alcohol tank and a waste liquid tank, wherein the needle washing pool is movably matched with the device frame, and the water storage tank, the alcohol tank and the waste liquid tank are fixedly connected with the device frame. The needle washing unit is used for washing the inner and outer arms of the needle point of the sample adding needle, so that mixing of different types of solutions, cross contamination and needle blocking are avoided, the needle washing pool is arranged to provide a place for washing the sample adding needle, the water storage tank is arranged to store water, the alcohol storage tank is arranged to store alcohol, and the waste liquid tank is arranged to collect waste liquid after washing the sample adding needle.

Preferably, the needle washing pool is provided with an overflow pipe, and the overflow pipe is connected and communicated with the waste liquid tank through a soft rubber pipe. An overflow pipe is arranged and communicated with the waste liquid tank through a soft rubber pipe to prevent liquid in the needle washing pool from overflowing.

Preferably, the needle washing unit further comprises a first filter and a second filter, the first filter and the second filter are fixedly connected with the device frame, the first filter is connected and communicated with the water storage tank through a soft rubber pipe, and the second filter is connected and communicated with the alcohol tank through a soft rubber pipe. The first filter and the second filter are arranged to filter clear water output by the water storage tank and alcohol output by the alcohol tank respectively, so that impurities are prevented from being entrained by the clear water or the alcohol and flowing into the sample adding needle, and the sample adding needle is blocked.

Preferably, the needle washing unit further comprises a first one-way valve and a second one-way valve, wherein the first one-way valve is connected and communicated with the first filter through a soft rubber tube, and the second one-way valve is connected and communicated with the second filter through a soft rubber tube. The first one-way valve is arranged to prevent clean water output by the water storage tank from flowing back to the water storage tank.

Preferably, the first one-way valve and the second one-way valve are connected and communicated with the plunger pump through a soft rubber tube. The second one-way valve is arranged to prevent the alcohol output by the alcohol tank from flowing back to the alcohol tank.

Preferably, the trace high-volatility liquid sample adding device further comprises a containing box, and the containing box is movably matched with the sample adding needle. The volatile cell mixed liquid can be stored by arranging the containing box.

Preferably, a box cover is arranged at the top of the containing box, and the box cover is connected with the containing box through threads in a ventilation way. The box cover is in threaded connection with the containing box, so that the box cover is in a closed state and an open state relative to the containing box, and the volatile cell mixed solution is prevented from being volatilized.

Preferably, the trace amount of high-volatility liquid sample adding device further comprises a plurality of fixing holes. The utility model can be fixed by arranging the fixing holes.

Compared with the prior art, the utility model has the following advantages:

according to the trace high-volatility liquid sample adding device, the connector is fixed at the top of the needle mounting seat, the connecting pipe is arranged at the bottom of the connector, so that the sample adding needle and the sealing piece fixed by the sample adding needle are sleeved on the outer wall of the connecting pipe and can slide up and down, the spring is used for connecting the connector and the sealing piece, so that the sample adding needle floats up and down corresponding to the needle mounting seat, the sample adding needle is prevented from colliding with a used object, the tip of the sample adding needle is ensured to be clung to the surface of a glass slide, the mixed liquid is ensured to be completely adsorbed by the surface tension of the glass slide, the plunger pump is communicated with the sample adding needle, and the sample adding needle is matched with the liquid level detection conducting piece, so that the liquid sucking and the liquid sucking of the sample adding needle are accurately controlled.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present utility model, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a cross-sectional view of the coupling of the sample application needle of the present utility model to a connector.

In the figure: 1. a device rack; 2. a sample adding unit; 3. a needle washing unit; 4. a soft rubber tube; 5. a plunger pump; 6. a sample adding needle; 7. a needle mount; 8. a liquid level detection conductive sheet; 9. a joint; 10. a connecting pipe; 11. a spring; 12. a seal; 13. a needle washing pool; 14. a water storage tank; 15. an alcohol tank; 16. a waste liquid tank; 17. an overflow pipe; 18. a first filter; 19. a second filter; 20. a first one-way valve; 21. a second one-way valve; 22. a holding box; 23. a box cover; 24. and a fixing hole.

Detailed Description

The technical scheme of the utility model is further specifically described below through specific embodiments and with reference to the accompanying drawings. It should be understood that the practice of the utility model is not limited to the following examples, but is intended to be within the scope of the utility model in any form and/or modification thereof.

In the present utility model, unless otherwise specified, all parts and percentages are by weight, and the equipment, materials, etc. used are commercially available or are conventional in the art. The methods in the following examples are conventional in the art unless otherwise specified. The components and devices in the following examples are, unless otherwise indicated, all those components and devices known to those skilled in the art, and their structures and principles are known to those skilled in the art from technical manuals or by routine experimentation.

In the following detailed description of embodiments of the utility model, reference is made to the accompanying drawings in which, for purposes of explanation, numerous specific details are set forth in order to provide a thorough understanding of the embodiments of the utility model, however, one or more embodiments may be practiced without these specific details.

As shown in figure 1, a trace high-volatility liquid sample adding device comprises a plurality of fixing holes 24, a device frame 1, a sample adding unit 2, a needle washing unit 3 and a plurality of soft rubber tubes 4, wherein the fixing holes 24 are arranged to fix the trace high-volatility liquid sample adding device, the device frame 1 is arranged to fix part of the sample adding unit 2 and the needle washing unit 3, the sample adding unit 2 comprises a plunger pump 5, a sample adding needle 6 with superfine needle diameter and a needle mounting seat 7, the sample adding needle 6 with superfine needle diameter can reduce surface tension and reduce liquid volatilization surface area, the plunger pump 5 provides power for the sample adding unit 2, the plunger pump 5 is arranged at one end of the device frame 1, the plunger pump 5 is fixedly connected with the device frame 1, the plunger pump 5 is connected with the needle mounting seat 7 through the soft rubber tubes 4, the sample adding needle 6 is arranged inside the needle mounting seat 7, the sample adding needle 6 is in sliding fit with the needle mounting seat 7 as shown in figure 2, a liquid level detection conducting strip 8 is fixed between the needle mounting seat 7 and the sample adding needle 6, the liquid level detection conducting strip 8 is electrically connected with the plunger pump 5, the needle mounting seat 7 is provided with a joint 9, a connecting pipe 10 and a spring 11, the joint 9 is arranged at the top of the needle mounting seat 7 corresponding to the sample adding needle 6, the needle mounting seat 7 is fixedly connected with the joint 9, the bottom of the joint 9 is provided with the connecting pipe 10, the connecting pipe 10 is fixedly connected and communicated with the joint 9, the sample adding needle 6 is sheathed outside the connecting pipe 10, the sample adding needle 6 is in sliding fit with the connecting pipe 10, a sealing piece 12 is arranged outside the sample adding needle 6, the sealing piece 12 is fixedly connected with the sample adding needle 6, the sealing piece 12 is in sliding fit with the connecting pipe 10, a spring 11 is arranged between the sealing piece 12 and the joint 9, the spring 11 is fixedly connected with the sealing piece 12 and the joint 9, the sample adding needle 6, the connecting pipe 10, the joint 9 and the plunger pump 5 are serially communicated, thereby the plunger pump 5 can control the sample adding needle 6 to absorb liquid, because the joint 9 is fixed at the top of the needle mounting seat 7, the sealing element 12 is fixed with the sample adding needle 6, and the sealing element 12 and the sample adding needle 6 are sleeved on the outer wall of the connecting pipe 10, when a spring 11 is arranged between the joint 9 and the sealing element 12, the sample adding needle 6 can float up and down, collision with a used object is avoided, the tip of the sample adding needle is tightly attached to the surface of a slide, the mixed solution is ensured to be adsorbed by the surface tension of the slide completely, the trace high-volatility liquid sample adding device further comprises a containing box 22, the containing box 22 is movably matched with the sample adding needle 6, the containing box 22 is arranged to store the volatile cell mixed solution, a box cover 23 is arranged at the top of the containing box 22, the box cover 23 is in threaded connection with the containing box 22 in a ventilation way, and the box cover 23 is in threaded connection with the containing box 22, so that the box cover 23 is in a closed state and an open state relative to the containing box 22, and volatilization of the volatile cell mixed solution is prevented.

The needle washing unit 3 comprises a needle washing pool 13, a water storage tank 14, an alcohol tank 15 and a waste liquid tank 16, the needle washing pool 13 is movably matched with the device frame 1, the water storage tank 14, the alcohol tank 15 and the waste liquid tank 16 are fixedly connected with the device frame 1, the needle washing unit 3 cleans the inner and outer arms of the needle tip of the sample adding needle 6 to avoid mixing of different types of solutions, cross contamination and needle blocking, the needle washing pool 13 is arranged to provide a place for cleaning the sample adding needle 6, the water storage tank 14 is arranged to store water, the alcohol storage tank is arranged to store alcohol, the waste liquid tank 16 is arranged to collect waste liquid after cleaning the sample adding needle 6, the needle washing pool 13 is provided with an overflow pipe 17, the overflow pipe 17 is connected and communicated with the waste liquid tank 16 through a flexible rubber pipe 4, the overflow pipe 17 is arranged and communicated with the waste liquid tank 16 to prevent liquid in the needle washing pool 13 from overflowing, the needle washing unit 3 further comprises a first filter 18 and a second filter 19, the first filter 18 and the second filter 19 are fixedly connected with the device frame 1, the first filter 18 is connected and communicated with the water storage tank 14 through the soft rubber tube 4, the second filter 19 is connected and communicated with the alcohol tank 15 through the soft rubber tube 4, the first filter 18 and the second filter 19 are respectively arranged to filter clean water output by the water storage tank 14 and alcohol output by the alcohol tank 15, the clean water or the alcohol is prevented from entraining impurities to flow into the sample adding needle 6, the sample adding needle 6 is blocked, the needle washing unit 3 further comprises a first check valve 20 and a second check valve 21, the first check valve 20 is connected and communicated with the first filter 18 through the soft rubber tube 4, the second check valve 21 is connected and communicated with the second filter 19 through the soft rubber tube 4, the first check valve 20 is arranged to prevent clean water output by the water storage tank 14 from flowing back to the water storage tank 14, the first check valve 20 and the second check valve 21 are both connected and communicated with the plunger pump 5 through the flexible rubber tube 4, and the second check valve 21 is arranged to prevent the alcohol output by the alcohol tank 15 from flowing back to the alcohol tank 15.

The utility model relates to a micro high-volatility liquid sample adding device, which has the implementation principle that: when a user wants to quantitatively and uniformly drip volatile mixed liquid on a glass slide to form a stable cell ring by using the utility model, firstly, the needle washing unit 3 is enabled to work, the alcohol tank 15 outputs alcohol, the alcohol tank is influenced by the second one-way valve 21, the plunger pump 5 pushes the alcohol into the joint 9 along the soft rubber tube 4 and then flows through the connecting tube 10 and the sample adding needle 6, then the sample adding needle 6 outputs the alcohol into the needle washing pool 13, thus finishing the alcohol disinfection work of the sample adding unit 2, dissolving residual cells on the surface wall of the sample adding needle 6, enabling the water storage tank 14 to output clear water, the water is influenced by the second one-way valve 21, the plunger pump 5 pushes the clear water into the joint 9 along the soft rubber tube 4 and then flows through the connecting tube 10 and the sample adding needle 6, so that the clear water is utilized to flush the channel of the sample adding unit 2, high-volatile liquid such as alcohol is cleared, then the alcohol is output into the needle washing pool 13, if the liquid content in the needle washing pool 13 is excessive, the liquid in the needle washing pool 13 is output into the needle washing pool 16, the liquid 17 is enabled to be output into the pot 16, the liquid is reserved for the air, the liquid is filtered by the air, the air is firstly pumped into the liquid storage tank, the air is reserved by the air, and then the liquid is sucked into the air by the air tank, and the air is completely filled into the air by the air tank, and the air is prevented from being sucked by the air, and the air tank, and the air is completely filled by the air tank.

The principles and embodiments of the present utility model have been described herein with reference to specific examples, the description of which is intended only to facilitate an understanding of the method of the present utility model and its core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the utility model can be made without departing from the principles of the utility model and these modifications and adaptations are intended to be within the scope of the utility model as defined in the following claims.

Claims

1. A trace high volatile liquid sample adding device, which is characterized in that: the trace high-volatility liquid sample adding device comprises a device frame (1), a sample adding unit (2), a needle washing unit (3) and a plurality of soft rubber tubes (4), wherein the sample adding unit (2) comprises a plunger pump (5), a sample adding needle (6) and a needle mounting seat (7), the plunger pump (5) is arranged at one end of the device frame (1), the plunger pump (5) is fixedly connected with the device frame (1), the plunger pump (5) is connected with the needle mounting seat (7) through the soft rubber tubes (4), the sample adding needle (6) is arranged inside the needle mounting seat (7), the sample adding needle (6) is in sliding fit with the needle mounting seat (7), a liquid level detection conducting sheet (8) is fixed between the needle mounting seat (7) and the sample adding needle (6), the liquid level detection conducting sheet (8) is electrically connected with the plunger pump (5), the needle mounting seat (7) is provided with a joint (9), a connecting tube (10) and a spring (11), the corresponding sample adding needle (6) is arranged at the needle mounting seat (7) through the soft rubber tubes (4), the joint (9) is fixedly connected with the joint (9) and fixedly connected with the joint (10), the sample adding needle (6) is sleeved outside the connecting pipe (10), the sample adding needle (6) is in sliding fit with the connecting pipe (10), a sealing piece (12) is arranged outside the sample adding needle (6), the sealing piece (12) is fixedly connected with the sample adding needle (6), the sealing piece (12) is in sliding fit with the connecting pipe (10), a spring (11) is arranged between the sealing piece (12) and the joint (9), and the spring (11) is fixedly connected with the sealing piece (12) and the joint (9).

2. The trace amount of high-volatility liquid sample application device of claim 1, wherein: the needle washing unit (3) comprises a needle washing tank (13), a water storage tank (14), an alcohol tank (15) and a waste liquid tank (16), wherein the needle washing tank (13) is movably matched with the device frame (1), and the water storage tank (14), the alcohol tank (15) and the waste liquid tank (16) are fixedly connected with the device frame (1).

3. The trace amount of high-volatility liquid sample application device of claim 2, wherein: the needle washing pool (13) is provided with an overflow pipe (17), and the overflow pipe (17) is connected and communicated with the waste liquid tank (16) through a flexible rubber pipe (4).

4. The trace amount of high-volatility liquid sample application device of claim 2, wherein: the needle washing unit (3) further comprises a first filter (18) and a second filter (19), the first filter (18) and the second filter (19) are fixedly connected with the device frame (1), the first filter (18) is connected and communicated with the water storage tank (14) through a soft rubber tube (4), and the second filter (19) is connected and communicated with the alcohol tank (15) through the soft rubber tube (4).

5. The trace amount of high volatility liquid sample application device of claim 4, wherein: the needle washing unit (3) further comprises a first one-way valve (20) and a second one-way valve (21), the first one-way valve (20) is connected and communicated with the first filter (18) through a soft rubber tube (4), and the second one-way valve (21) is connected and communicated with the second filter (19) through the soft rubber tube (4).

6. The trace amount of high volatility liquid sample application device of claim 5, wherein: the first check valve (20) and the second check valve (21) are connected and communicated with the plunger pump (5) through a soft rubber tube (4).

7. The trace amount of high volatility liquid sample application device of claim 6, wherein: the trace high-volatility liquid sample adding device also comprises a containing box (22), and the containing box (22) is movably matched with the sample adding needle (6).

8. The trace amount of high volatility liquid sample application device of claim 7, wherein: the top of the containing box (22) is provided with a box cover (23), and the box cover (23) is in ventilation and threaded connection with the containing box (22).

9. The trace amount of high volatility liquid sample application device of claim 8, wherein: the trace high-volatility liquid sample adding device also comprises a plurality of fixing holes (24).