CN214297473U - Waste liquid collection device of nanometer enzyme immunoassay appearance - Google Patents

Abstract

The utility model relates to the technical field of immunochromatography equipment, in particular to a waste liquid collecting device of a nano enzyme immunoassay analyzer, which comprises a collecting unit and a middle unit; the collecting unit comprises a waste liquid collecting tank, the middle unit comprises a joint, and a peristaltic pump is arranged between the waste liquid collecting tank and the joint; the joint comprises a first communicating pipe, a second communicating pipe and an accommodating groove for inserting and fixing the waste liquid needle; the waste liquid collecting tank is communicated with the waste liquid needle through a first communicating pipe; a first channel and a second channel are arranged in the joint, the accommodating groove and the first communicating pipe are respectively communicated with the first channel, and the second communicating pipe is communicated with the second channel; when the second channel is communicated with the first channel, the second communicating pipe is used for conveying the cleaning liquid to the waste liquid collecting tank. The device of this scheme both can collect the waste liquid, can also carry out the washing of pipeline, collects two kinds of functions in an organic whole, has made things convenient for operation flow.

Description

Waste liquid collection device of nanometer enzyme immunoassay appearance

Technical Field

The utility model relates to an immunochromatography equipment technical field, concretely relates to nanometer enzyme immunoassay appearance's waste liquid collection device.

Background

The nano enzyme is formed by metal oxide and other materials (particularly iron-based materials) with the particle size reaching the nano level, and is a mimic enzyme with peroxidase and other activities. The nano enzyme can be used as a chromogenic catalyst to increase the sensitivity and accuracy of molecular detection. The patent CN110488002A (an immunochromatography device for conveniently adding liquid) previously filed by the same company discloses an immunochromatography device suitable for nano-enzyme based, which can realize automatic sample adding and detection of samples. In the color development process, color development liquid needs to be added into an observation window of the immunochromatography test paper box, and after the color development reaction is finished, the reacted color development liquid (waste liquid) needs to be sucked out by using a waste liquid needle, so that the immunochromatography test paper box can be analyzed in the next step. However, due to the existence of some substances such as antigens, antibodies, blood cells and the like in the observation window, the waste liquid sucked out from the observation window easily blocks the pipeline, and a device which can conveniently collect the waste liquid and can timely clean and dredge the pipeline needs to be designed.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the intention is providing a waste liquid collection device of nanometer enzyme immunoassay appearance, has solved the technical problem who lacks the waste liquid collection device who has mediation and scavenge pipe function.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a waste liquid collecting device of a nano enzyme immunoassay analyzer comprises a collecting unit and a middle unit; the collecting unit comprises a waste liquid collecting tank, the middle unit comprises a joint, and a peristaltic pump is arranged between the waste liquid collecting tank and the joint; the joint comprises a first communicating pipe, a second communicating pipe and an accommodating groove; a waste liquid needle is inserted into the accommodating tank, and the waste liquid collecting tank is communicated with the waste liquid needle through a first communicating pipe; a first channel and a second channel are arranged in the joint, the accommodating groove and the first communicating pipe are respectively communicated with the first channel, and the second communicating pipe is communicated with the second channel; when the second channel is communicated with the first channel, the second communicating pipe is used for conveying the cleaning liquid to the waste liquid collecting tank.

The application method, the principle and the advantages of the scheme are as follows: fix the waste liquid needle on the reaction of prior art immunochromatography device is placed the board earlier, then in the holding tank with the upper end insert joint of waste liquid needle. The waste liquid collecting tank is communicated with the first communicating pipe, the second channel is controlled to be in a state of being not communicated with the first channel, the peristaltic pump is started, waste liquid can be sucked into the waste liquid collecting tank by the waste liquid according to the waste color developing liquid (waste liquid) in the observation window of the reagent card box. When the pipeline is required to be cleaned, the second channel and the first channel are controlled to be in a communicated state, the peristaltic pump is started, and the cleaning liquid is conveyed to the waste liquid collecting tank through the second communicating pipe. Therefore, the cleaning effect on the pipeline is realized, and substances such as broken blood cells, macromolecular proteins and the like in the pipeline can be washed and cleaned, so that the substances are prevented from accumulating for a long time to block the pipeline and a peristaltic pump. In addition, the waste liquid needle is tiny and easy to damage and block, the joint and the waste liquid needle can be detached, and the waste liquid needle can be conveniently taken down from the joint and replaced when the waste liquid needle is damaged or blocked.

In the scheme, the cleaning liquid is not directly input from the waste liquid needle, because the waste liquid needle is thin, the structure of the waste liquid needle is suitable for absorbing the waste color developing liquid, however, the cleaning liquid is absorbed by the waste liquid needle, so that the entering amount of the cleaning liquid is too small, and the flow rate of the cleaning liquid in the whole pipeline is limited. The liquid flow rate requirements for cleaning the lines and extracting the waste liquid are different. During cleaning, cleaning liquid is needed to wash the pipeline wall, so that dirt (such as antigen, antibody, blood cells and the like) is removed. Therefore, the second communicating pipe is particularly arranged in the scheme, so that the phenomenon of poor cleaning effect caused by the undersize of the waste liquid needle diameter can be avoided.

To sum up, the waste liquid collection device of this scheme both can collect the waste liquid that comes from the waste liquid needle, can also realize wasing the pipeline and absorb the switching between the waste liquid through the intercommunication relation that switches the joint, collects two kinds of functions in an organic whole, has made things convenient for operation flow.

Preferably, as an improvement, the cleaning device further comprises a liquid supply unit, wherein the liquid supply unit comprises a cleaning liquid storage tank and a cleaning liquid output pipe; and one end of the cleaning liquid output pipe is inserted into the cleaning liquid storage tank, and the other end of the cleaning liquid output pipe is communicated with the second communicating pipe.

By adopting the technical scheme, the liquid supply unit can provide stable supply of the cleaning liquid for the joint.

Preferably, as an improvement, a vertical channel is arranged in the joint, and a liquid path control mechanism for communicating the second channel with the first channel is inserted into the vertical channel.

By adopting the technical scheme, whether the second channel is communicated with the first channel or not can be controlled through the liquid path control mechanism, so that the conversion between cleaning a pipeline and absorbing waste liquid is realized.

Preferably, as an improvement, the fluid path control mechanism includes a flow-limiting post inserted into the vertical channel and configured to rotate, and a connecting channel configured to communicate the first channel with the second channel is provided on the flow-limiting post.

Adopt above-mentioned technical scheme, can realize the intercommunication of first passageway and second passageway intercommunication through the mode of rotating the current-limiting column to realize the conversion between waste liquid needle imbibition and the absorption washing liquid, convenient operation.

Preferably, as an improvement, a rubber tube is fixed on a side wall of the accommodating groove; the one end that is close to first passageway of holding tank is equipped with the sealing washer.

By adopting the technical scheme, the waste liquid needle is inserted into the rubber tube, and the rubber tube exerts certain friction force on the waste liquid needle to prevent the waste liquid needle from sliding down. The setting of sealing washer can prevent the condition of weeping from appearing between waste liquid needle and the joint.

Preferably, as an improvement, be fixed with the collection tank upper cover on the waste liquid collection tank, be fixed with level sensor and bee calling organ on the collection tank upper cover.

By adopting the technical scheme, the waste color developing liquid (waste liquid) is conveyed into the waste liquid collecting tank, when the liquid level is over the detection end of the liquid level sensor, the buzzer sounds to prompt a user to notice that the waste liquid collecting tank is filled, the waste liquid needs to be treated in time, and the overflow of the waste liquid is avoided.

Drawings

FIG. 1 is a perspective view of a prior art waste needle and its manner of installation.

Fig. 2 is a front view of the collection unit of embodiment 1.

Fig. 3 is a longitudinal sectional view of the joint of example 1.

Fig. 4 is a front view of the fluid path control mechanism of embodiment 1.

Fig. 5 is a front view of the liquid supply unit of embodiment 2.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: board 1 is placed in the reaction, waste liquid needle 2, liquid feeding needle 3, reagent draw-in groove 4, reagent card box 5, connect 6, first communicating pipe 7, second communicating pipe 8, first passageway 9, second passageway 10, vertical passageway 11, sealing washer 12, rubber tube 13, twist grip 14, current-limiting post 15, spliced pole 16, connecting channel 17, waste liquid collection tank 18, collection tank upper cover 19, waste liquid output tube 20, waste liquid input tube 21, peristaltic pump 22, intermediate pipe 23, level sensor 24, bee calling organ 25, washing liquid output tube 26, storage tank upper cover 27, feed liquor hole 28, washing liquid storage tank 29, stagnant water clamp 30.

Example 1

FIG. 1 is a view from patent CN110488002A (an immunochromatographic device for facilitating the addition of liquid), showing the installation position of a waste liquid needle 2. Waste liquid needle 2 is blocked on reaction is placed board 1, and reaction is placed and still is fixed with liquid feeding needle 3 on the board 1, and reagent draw-in groove 4 drives reagent card box 5 and moves under liquid feeding needle 3, through liquid feeding needle 3 to the observation window dropwise add color development liquid of reagent card box 5, after accomplishing the color development, by waste liquid needle 2 with color development liquid suction, just so can read the testing result at the observation window of reagent card box 5. Waste liquid needle 2 is the equal open-ended cylinder tubular structure from top to bottom, and this scheme improves on waste liquid needle 2 and its fixed knot constructs (the reaction is placed board 1), has designed one set and has helped waste liquid absorption and liquid way abluent structure, specifically as follows:

referring to fig. 2 and 3, the waste liquid collecting device of the nanoenzyme immunoassay analyzer comprises a collecting unit and an intermediate unit, wherein the collecting unit comprises a waste liquid collecting tank 18 (fixed on the base of the immunochromatographic device in CN110488002A by a screw), the intermediate unit comprises a joint 6, and the joint 6 and the waste liquid collecting tank 18 are communicated by a pipeline (described in detail later). As shown in fig. 2, a collection tank upper cover 19 is fixed on the waste liquid collection tank 18 through screws, a liquid level sensor 24(FS-IR1902D PNP NC, EPT/energy point) is arranged on the collection tank upper cover 19 in a penetrating manner and is fixed through screws, and a buzzer 25(zmq-2724, dc6-24v electronic miniature alarm, which is powered by a battery, is fixed on the upper surface of the collection tank upper cover 19 through screws, wherein the buzzer 25 is controlled by a relay to be turned on and off). This scheme selects for use STM32 singlechip (STM32F103 chip) control level sensor 24 and bee calling organ 25, STM32F103 chip receives level sensor 24's signal of telecommunication, when the liquid level reaches the probe end contact of take the altitude with level sensor 24, the signal of telecommunication that level sensor 24 sent to STM32F103 chip changes, next, the level (for example high level) that STM32F103 chip passed through I/O mouth output change, control relay switch-on bee calling organ 25 circuit, bee calling organ 25 reports to the police. The STM32F103 chip and the liquid level sensor 24 are both powered by a self-contained power supply of the immunochromatography device disclosed in the patent CN110488002A, and can also be powered by commercial power. The lateral wall of the waste liquid collecting tank 18 is communicated with a waste liquid input pipe 21 and a waste liquid output pipe 20, the waste liquid collecting tank 18 is provided with holes for the waste liquid input pipe 21 and the waste liquid output pipe 20 to penetrate, and the waste liquid input pipe 21 and the waste liquid output pipe 20 are both fixedly bonded with the waste liquid collecting tank 18. The waste liquid output pipe 20 is provided with a water stop clamp 30 (a clamp clamped on a silicone tube for preventing water from flowing, and a valve commonly used in the prior art can also be arranged in the waste liquid output pipe 20) in the prior art. The end of the waste liquid input tube 21 far away from the waste liquid collecting tank 18 is communicated with the water outlet end of a peristaltic pump 22 (Jiegeng 104KA, 24v, the immunochromatography device is provided with a power supply for supplying power), and the water inlet end of the peristaltic pump 22 is communicated with an intermediate tube 23. The end of the intermediate tube 23 remote from the peristaltic pump 22 is used to communicate with the fitting 6.

As shown in fig. 3, the joint 6 is provided with a vertically arranged cylindrical vertical channel 11, the left side of the joint 6 is provided with a horizontally arranged cylindrical first channel 9, the right side of the joint 6 is provided with a horizontally arranged cylindrical second channel 10, and both the first channel 9 and the second channel 10 are communicated with the vertical channel 11. The first channel 9 and the second channel 10 are coaxial and have the same inner diameter, the inner diameter of the vertical channel 11 is larger than that of the first channel 9 and the second channel 10, and the central axes of the vertical channel 11, the first channel 9 and the second channel 10 are on the same plane. A liquid path control mechanism shown in fig. 4 is inserted into the vertical channel 11, the liquid path control mechanism comprises a flow limiting column 15, a connecting channel 17 horizontally penetrating through the flow limiting column 15 is arranged in the flow limiting column 15, a connecting column 16 is integrally formed at the upper end of the flow limiting column 15, and a rotating handle 14 is integrally formed at the upper end of the connecting column 16. When the flow limiting device is used, the flow limiting column 15 is inserted into the vertical channel 11, the rotating handle 14 and the connecting column 16 are positioned outside the joint 6, and the flow limiting column 15 is rotated through the rotating handle 14, so that the connecting channel 17 can communicate the first channel 9 with the second channel 10; it is also possible to misalign the connecting channel 17 from the first channel 9 and the second channel 10, the first channel 9 and the second channel 10 being spaced apart. In order to increase the sealing performance, a layer of vaseline can be coated on the outer surface of the flow limiting column 15, and the outer diameter of the flow limiting column 15 is slightly smaller than the inner diameter of the vertical channel 11, so as to ensure that the flow limiting column 15 can rotate. The left side of the first channel 9 is communicated with a first communicating pipe 7, the first communicating pipe 7 and the first channel 9 are coaxial, and the first communicating pipe 7 is fixedly bonded on the joint 6; the right side of the second channel 10 is communicated with a second communicating pipe 8, the second communicating pipe 8 and the second channel 10 are coaxial, and the second communicating pipe 8 is fixedly bonded on the joint 6. The first communication pipe 7 is inserted into an end of the intermediate pipe 23 remote from the peristaltic pump 22 so that the first communication pipe 7 and the intermediate pipe 23 communicate. The lower part of the first channel 9 is provided with a holding tank of the waste liquid needle 2, and the holding tank is of a cylindrical structure matched with the shape of the waste liquid needle 2. The side wall of the holding tank is bonded and fixed with a rubber tube 13 for preventing the waste liquid needle 2 from sliding off. The holding tank passes through middle part intercommunicating pore and first passageway 9 intercommunication to be equipped with sealing washer 12 in the upper end of holding tank, the through-hole of the same size as middle part intercommunicating pore is seted up at sealing washer 12 middle part. When the waste liquid needle 2 is connected by the joint 6, the waste liquid needle 2 is inserted into the housing groove until the upper end of the waste liquid needle 2 is pressed against the seal ring 12.

The specific implementation process comprises the following steps: waste liquid needle 2 is fixed on reaction placing plate 1, then insert waste liquid needle 2's upper end in the holding tank of joint 6, then be connected intermediate pipe 23 and first communicating pipe 7, rotate current-limiting post 15 to the state that connecting channel 17 does not communicate with first passageway 9 and second passageway 10. When the peristaltic pump 22 is started, the waste liquid needle 2 can absorb the color developing liquid in the observation window of the reagent card box 5, the waste color developing liquid (waste liquid) is conveyed into the waste liquid collecting tank 18, when the liquid level is over the detection end of the liquid level sensor 24, the buzzer 25 sounds to prompt a user to notice that the waste liquid collecting tank 18 is full, the water stop clamp 30 needs to be taken down, the waste liquid output pipe 20 is opened, so that the waste liquid flows out and is collected, and then the waste liquid is subjected to harmless treatment. When the pipeline cleaning is needed, the flow limiting column 15 is rotated by rotating the handle 14, so that the connecting channel 17 communicates the first channel 9 with the second channel 10, and the waste liquid needle 2 is covered (sealed) at one end away from the joint 6 by using a rubber cover matched with the waste liquid needle 2. Then, the second communicating pipe 8 is communicated with a prior art liquid conveying pipe, and the liquid conveying pipe is communicated with a container for containing cleaning liquid. When the peristaltic pump 22 is started, the cleaning fluid is sucked into the channel in the joint 6, the waste fluid input tube 21, the intermediate tube 23 and the like, and substances such as broken blood cells, macromolecular proteins and the like in the pipeline can be cleaned, so that the substances are prevented from accumulating for a long time to block the pipeline and the peristaltic pump 22. In addition, waste liquid needle 2 is more tiny in this scheme, easy damage and jam, connects 6 and waste liquid needle 2 can be dismantled, conveniently when waste liquid needle 2 is damaged or is blockked up, takes off waste liquid needle 2 from connecting 6 and change.

Example 2

This embodiment is an improvement of embodiment 1, in which a liquid supply unit is additionally provided. As shown in FIG. 5, the liquid supply unit comprises a cleaning solution storage tank 29 (fixed on the base of the immunochromatographic device in CN110488002A by screws), an upper storage tank cover 27 is fixed on the upper end of the cleaning solution storage tank 29 by screws, a cleaning solution output pipe 26 is arranged in the upper storage tank cover 27 in a penetrating way, and a liquid inlet hole 28 is also arranged on the upper storage tank cover 27. The cleaning solution in the cleaning solution storage tank 29 may be clean water, and in order to increase the cleaning effect, a surfactant may be added to the clean water to increase the cleaning effect on the dirt (cell membrane, chromogenic substance, protein, etc.) adsorbed in the pipeline. In order to observe whether the joint 6 is blocked or not and to observe the flowing condition of the liquid, the joint 6 may be made of transparent glass.

While the embodiments of the present invention have been described in detail with reference to the accompanying drawings, it is to be understood that the present invention is not limited to the embodiments and that various changes and modifications may be made therein by those skilled in the art without departing from the spirit and scope of the invention. These should also be considered as the scope of protection of the present invention, and these do not affect the effect of the implementation of the present invention and the utility of the patent. The techniques, shapes, and structural parts, which are omitted from the description of the present invention, are all known techniques.

Claims (6)

1. A waste liquid collecting device of a nano enzyme immunoassay analyzer is characterized by comprising a collecting unit and a middle unit; the collecting unit comprises a waste liquid collecting tank, the middle unit comprises a joint, and a peristaltic pump is arranged between the waste liquid collecting tank and the joint; the joint comprises a first communicating pipe, a second communicating pipe and an accommodating groove; a waste liquid needle is inserted into the accommodating tank, and the waste liquid collecting tank is communicated with the waste liquid needle through a first communicating pipe; a first channel and a second channel are arranged in the joint, the accommodating groove and the first communicating pipe are respectively communicated with the first channel, and the second communicating pipe is communicated with the second channel; when the second channel is communicated with the first channel, the second communicating pipe is used for conveying the cleaning liquid to the waste liquid collecting tank.

2. The waste liquid collecting device of the nanoenzyme immunoassay analyzer as defined in claim 1, further comprising a liquid supply unit, wherein the liquid supply unit comprises a cleaning liquid storage tank and a cleaning liquid output pipe; and one end of the cleaning liquid output pipe is inserted into the cleaning liquid storage tank, and the other end of the cleaning liquid output pipe is communicated with the second communicating pipe.

3. The waste liquid collecting device of the nanoenzyme immunoassay analyzer as set forth in claim 2, wherein a vertical channel is provided in the joint, and a liquid path control mechanism for communicating the second channel with the first channel is inserted in the vertical channel.

4. The waste liquid collecting device of the nanoenzyme immunoassay analyzer as set forth in claim 3, wherein the liquid path control mechanism comprises a flow restriction column inserted into the vertical channel and adapted to rotate, and the flow restriction column is provided with a connecting channel for communicating the first channel with the second channel.

5. The waste liquid collecting device of the nanoenzyme immunoassay analyzer as set forth in claim 4, wherein a rubber tube is fixed to a side wall of the housing groove; the one end that is close to first passageway of holding tank is equipped with the sealing washer.

6. The apparatus of claim 5, wherein the upper cover of the collecting tank is fixed on the waste liquid collecting tank, and the liquid level sensor and the buzzer are fixed on the upper cover of the collecting tank.

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