CN108884154A - 具有独特剪接活性的断裂内含肽 - Google Patents

具有独特剪接活性的断裂内含肽 Download PDF

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Publication number
CN108884154A
CN108884154A CN201780021267.0A CN201780021267A CN108884154A CN 108884154 A CN108884154 A CN 108884154A CN 201780021267 A CN201780021267 A CN 201780021267A CN 108884154 A CN108884154 A CN 108884154A
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seq
intein
fragment
split intein
amino acid
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CN201780021267.0A
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Chinese (zh)
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T·W·缪尔
A·J·史蒂芬
N·H·沙赫
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Princeton University
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Princeton University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
CN201780021267.0A 2016-01-29 2017-01-27 具有独特剪接活性的断裂内含肽 Pending CN108884154A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662288661P 2016-01-29 2016-01-29
US62/288,661 2016-01-29
PCT/US2017/015455 WO2017132580A2 (en) 2016-01-29 2017-01-27 Split inteins with exceptional splicing activity

Publications (1)

Publication Number Publication Date
CN108884154A true CN108884154A (zh) 2018-11-23

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CN201780021267.0A Pending CN108884154A (zh) 2016-01-29 2017-01-27 具有独特剪接活性的断裂内含肽

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US (2) US11142550B2 (https=)
EP (2) EP4234689A3 (https=)
JP (1) JP7290305B2 (https=)
CN (1) CN108884154A (https=)
AU (1) AU2017211395B2 (https=)
CA (1) CA3051195A1 (https=)
DK (1) DK3408292T3 (https=)
ES (1) ES2949163T3 (https=)
FI (1) FI3408292T3 (https=)
HU (1) HUE062276T2 (https=)
PL (1) PL3408292T3 (https=)
PT (1) PT3408292T (https=)
SI (1) SI3408292T1 (https=)
WO (1) WO2017132580A2 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110714020A (zh) * 2019-05-22 2020-01-21 北京理工大学 一种高效简便纯化蛋白的方法
WO2021047559A1 (zh) * 2019-09-09 2021-03-18 武汉友芝友生物制药有限公司 一种断裂型内含肽、使用其的重组多肽的制备方法
WO2021179860A1 (zh) * 2020-03-11 2021-09-16 梦芊科技知识产权有限公司 利用枯草芽孢杆菌和核酸内切酶制备人碱性成纤维细胞生长因子
CN113735941A (zh) * 2021-08-13 2021-12-03 江南大学 一种蛋白纯化方法及其应用
CN114698379A (zh) * 2019-11-22 2022-07-01 思拓凡生物工艺研发有限公司 使用断裂内含肽系统的的蛋白质纯化
US12529041B2 (en) 2018-09-07 2026-01-20 Beam Therapeutics Inc. Compositions and methods for delivering a nucleobase editing system

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JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
CA2909733C (en) 2013-04-18 2023-10-17 Fondazione Telethon Effective delivery of large genes by dual aav vectors
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3322800A4 (en) 2015-07-13 2019-05-01 University of Utah Research Foundation PROCESS FOR THE PRODUCTION OF RED BLOOD BODIES AND BLOOD PLATES IN VITRO AND USES THEREOF
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
ES2949163T3 (es) * 2016-01-29 2023-09-26 Univ Princeton Inteínas divididas con actividad de corte y empalme excepcional
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) * 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
AU2019316094B2 (en) 2018-08-03 2026-02-19 Beam Therapeutics Inc. Multi-effector nucleobase editors and methods of using same to modify a nucleic acid target sequence
US11530245B2 (en) * 2018-08-31 2022-12-20 Washington University Split intein mediated polymerization and production of mussel foot adhesive protein materials
EP3850088A4 (en) 2018-09-07 2023-07-19 Beam Therapeutics, Inc. Compositions and methods for improving base editing
AU2019360372B2 (en) * 2018-10-15 2026-02-26 Fondazione Telethon Intein proteins and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
LU101118B1 (en) * 2019-02-06 2020-08-06 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Method for detecting a specific splice event of a gene of interest
WO2020168122A1 (en) 2019-02-13 2020-08-20 Beam Therapeutics Inc. Modified immune cells having adenosine deaminase base editors for modifying a nucleobase in a target sequence
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
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CA3152679A1 (en) * 2019-08-28 2021-03-04 The Trustees Of Princeton University Atypical split inteins and uses thereof
EP4034138A4 (en) 2019-09-27 2024-07-31 Beam Therapeutics, Inc. COMPOSITIONS AND METHODS FOR THE TREATMENT OF LIQUID CANCERS
KR20220070481A (ko) * 2019-10-01 2022-05-31 유타대학연구재단 순환 종양 세포를 표적화하기 위한 혈소판 조작 방법
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KR102663243B1 (ko) * 2021-08-30 2024-05-03 국립순천대학교산학협력단 트립신 특이적 형광 프로브 및 이의 용도
JP2024533311A (ja) 2021-09-08 2024-09-12 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物
WO2023081714A1 (en) * 2021-11-02 2023-05-11 Cornell University Intein systems and uses thereof
WO2023229029A1 (ja) * 2022-05-26 2023-11-30 国立大学法人山形大学 ヘテロダイマータンパク質の製造方法、ダイマータンパク質、モノマータンパク質、および標的反応性のヘテロダイマータンパク質のスクリーニング方法
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AU2024338787A1 (en) 2023-09-04 2026-03-19 Splicebio, Sl Use of split intein for the treatment of scn1a associated disease
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529041B2 (en) 2018-09-07 2026-01-20 Beam Therapeutics Inc. Compositions and methods for delivering a nucleobase editing system
CN110714020A (zh) * 2019-05-22 2020-01-21 北京理工大学 一种高效简便纯化蛋白的方法
CN110714020B (zh) * 2019-05-22 2021-08-03 北京理工大学 一种高效简便纯化蛋白的方法
WO2021047559A1 (zh) * 2019-09-09 2021-03-18 武汉友芝友生物制药有限公司 一种断裂型内含肽、使用其的重组多肽的制备方法
WO2021047558A1 (zh) * 2019-09-09 2021-03-18 武汉友芝友生物制药有限公司 断裂型内含肽、使用其的重组多肽的制备方法
CN114698379A (zh) * 2019-11-22 2022-07-01 思拓凡生物工艺研发有限公司 使用断裂内含肽系统的的蛋白质纯化
CN114698379B (zh) * 2019-11-22 2024-12-27 思拓凡生物工艺研发有限公司 使用断裂内含肽系统的蛋白质纯化
WO2021179860A1 (zh) * 2020-03-11 2021-09-16 梦芊科技知识产权有限公司 利用枯草芽孢杆菌和核酸内切酶制备人碱性成纤维细胞生长因子
CN113735941A (zh) * 2021-08-13 2021-12-03 江南大学 一种蛋白纯化方法及其应用
CN113735941B (zh) * 2021-08-13 2024-01-30 江南大学 一种蛋白纯化方法及其应用

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WO2017132580A3 (en) 2017-09-14
ES2949163T3 (es) 2023-09-26
US20200055900A1 (en) 2020-02-20
EP3408292A4 (en) 2019-09-18
JP7290305B2 (ja) 2023-06-13
US11142550B2 (en) 2021-10-12
JP2019506163A (ja) 2019-03-07
CA3051195A1 (en) 2017-08-03
PT3408292T (pt) 2023-07-19
SI3408292T1 (sl) 2023-09-29
HUE062276T2 (hu) 2023-10-28
DK3408292T3 (da) 2023-06-12
AU2017211395A1 (en) 2018-09-06
EP4234689A3 (en) 2023-09-13
AU2017211395B2 (en) 2024-04-18
PL3408292T3 (pl) 2023-10-02
EP3408292B1 (en) 2023-04-26
EP3408292A2 (en) 2018-12-05
WO2017132580A2 (en) 2017-08-03
US20210371467A1 (en) 2021-12-02
EP4234689A2 (en) 2023-08-30
US12297472B2 (en) 2025-05-13
FI3408292T3 (fi) 2023-06-30

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