CN108849477A - High-quality selection and application without climing seed with musky gourd kind - Google Patents

High-quality selection and application without climing seed with musky gourd kind Download PDF

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Publication number
CN108849477A
CN108849477A CN201810770515.8A CN201810770515A CN108849477A CN 108849477 A CN108849477 A CN 108849477A CN 201810770515 A CN201810770515 A CN 201810770515A CN 108849477 A CN108849477 A CN 108849477A
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China
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climing
seed
long
musky gourd
plate
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Inventor
钱奕道
张领
袁刚
葛继涛
张从合
杨坤
陈孝奇
乔蓉蓉
马辉
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Anhui An Aromatic Plant Metioned In Ancient Books Silver High-Tech Seed Of Vegetables And Fruits Co Ltd
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Anhui An Aromatic Plant Metioned In Ancient Books Silver High-Tech Seed Of Vegetables And Fruits Co Ltd
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Priority to CN201810770515.8A priority Critical patent/CN108849477A/en
Publication of CN108849477A publication Critical patent/CN108849477A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention provides a kind of high-quality selection and application without climing seed musky gourd kind, is related to crop breeding technical field.Use without climing oblate spheroid musky gourd homozygosis materials A and trait expression as the long 2.5cm or more of seed plate, plate width is 1.3cm or more or single fruit kernal number 520 or more climing musky gourd homozygosis material B of length, use parent B to be continuously preferably returned as recurrent parent, be selfed and obtain BCn+1Fm+1, choose without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or moren+ 1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, and homozygous inbred lines material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;Provide a seed musky gourd specific breed, the merit of three kinds of parents of the kind set, have plant without it is climing, single fruit kernal number 350 or more, plate is long, plate is wide, 100-grain weight show it is excellent, conducive to dense planting, considerable economic benefit can be brought for enterprise and peasant household.

Description

High-quality selection and application without climing seed with musky gourd kind
Technical field
The present invention relates to crop breeding technical fields, and in particular to a kind of high-quality without the choosing of musky gourd kind of climing seed Educate method and application.
Background technique
Seed-use pumpkin is the traditional agricultural and sideline product in China and foreign trade commodity using seed as main edible organs.Pumpkin Essential fatty acid, amino acid, phytosterol, minerals, vitamin and polysaccharide rich in etc., have drive away helminth, The effects of reducing cholesterol, anti-inflammatory, anti-oxidant, alleviation hypertension, reduction bladder and urethra pressure.
Existing seed-use pumpkin can be divided into musky gourd, giant pumpkin, american pumpkin etc., product be referred to as flash, Blank or snow-white, tabula rasa.Seed-use pumpkin is long in China's developing history, and especially in nearly development in 30 years, seed-use pumpkin is produced Industry forms the industrial system of " breeding, production, processing, popularization, marketing integration ".
But existing more seed-use pumpkin kind, mainly giant pumpkin (blank) and America are applied in production Pumpkin (tabula rasa), musky gourd (flash) there is no seed specific breed at present, and the breeding for being engaged in this respect specific breed does not appear in the newspapers Road.It is available without specific breed in production at present, cause the enterprise's raw material quality for being engaged in the processing of the type pumpkin irregular, The product quality fluctuation processed is big, seriously affects enterprise's health, orderly development.Therefore, the excellent seed China of Breeding Traits Pumpkin specific breed increases economic results in society, promotes the development of China's pumpkin industrialization to have non-for meeting market supply Often important meaning.
Summary of the invention
(1) the technical issues of solving
In view of the shortcomings of the prior art, the present invention provides a kind of high-quality selection without climing seed musky gourd kind and answers With solving the technical issues of seed musky gourd is without specific breed in the prior art.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs:
It is a kind of high-quality without the climing seed selection of musky gourd kind, include the following steps:
S1:It chooses miscellaneous as parent without climing oblate spheroid musky gourd homozygosis materials A and long climing musky gourd homozygosis material B It hands over, obtains F1;
S2:It is returned the parent B as recurrent parent and the F1, obtains BC1
S3:BC1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit Seed number 450 or more BC1Single plant uses the parent B as recurrent parent continuous backcross, continuously preferably obtains BCn+1
S4:BCn+1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit Seed number 450 or more BCn+1Single plant is selfed, and BC is obtainedn+1Fm+1
S5:It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or more BCn+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C;
S6:The homozygous inbred lines material C hybridizes to obtain without climing seed South China with long climing musky gourd homozygosis material E Melon;
Wherein, n is natural number, and n≤1, m are natural number, and m≤1;
The trait expression of the climing musky gourd homozygosis material B and E of length is the long 2.5cm or more of seed plate, plate width is 1.3cm or more or single fruit kernal number 520 or more.
Preferably, the climing musky gourd homozygosis material B of the length is that long climing long tubular musky gourd homozygosis material or length are climing One of oblate spheroid musky gourd homozygosis material;The climing musky gourd homozygosis material E of length is long climing long tubular musky gourd One of homozygous material or long climing oblate spheroid musky gourd homozygosis material.
Preferably, the trait expression of the climing oblate spheroid musky gourd homozygosis material of the length be oblate spheroid, the long 2.5cm of plate with Upper, the wide 1.3cm or more of plate, 100-grain weight 35g or more, single fruit kernal number 300 or more;
The trait expression of the climing long tubular musky gourd homozygosis material of length is that long tubular, the long 1.7cm or more of plate, plate are wide 0.9cm or more, 100-grain weight 16g or more, 520 or more single fruit kernal number.
Preferably, F is obtained in step S11Crossing system be A × B or B × A;It obtains in the step S6 without climing seed It is C × E or E × C with the Crossing system of musky gourd.
Preferably, BC is obtained in step S3n+1Backcrossing mode be according to north and south region ecological condition otherness carry out it is different Ground is continuously preferred.
Preferably, BC is obtained in step S4n+1Fm+1Selfing mode be according to north and south region ecological condition otherness into Row strange land is continuously preferred.
Preferably, the n is 1-8, m 1-10.
It high-quality is educated with the selection of musky gourd kind in pumpkin without climing seed the present invention also provides a kind of using above-mentioned Application in kind.
(3) beneficial effect
The present invention provides a kind of high-quality selection and application without climing seed musky gourd kind, compared with prior art Advantage is:
1, the present invention use without climing oblate spheroid musky gourd homozygosis materials A and trait expression for the long 2.5cm or more of seed plate, Plate width is the climing musky gourd homozygosis material B of length of 1.3cm or more or single fruit kernal number 520 or more, uses parent B as circulation Parent is continuously preferably returned, selfing obtains BCn+1Fm+1, choose without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or The BC of single fruit seed number 450 or moren+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, homozygous inbred lines material Material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;One seed musky gourd special product is provided Kind, this without climing seed with the merit of three kinds of parents of musky gourd set, have plant without climing, growing way is steady, female flower It is more, easily bear fruit, single fruit kernal number 350 or more, plate is long, plate is wide, 100-grain weight shows excellent, single fruit weight 3kg or more, yield Height is conducive to dense planting, can bring considerable economic benefit for enterprise and peasant household.
2, the present invention is continuously preferred according to the otherness progress strange land of north and south region ecological condition.North and south region latitude is not Together, sunshine duration is different, selects the insensitive single plant of counterglow using continuous preferred mode.So that finally obtained nothing Climing seed musky gourd enhances natural environment adaptability, and plantation region is wider.
3, the present invention completes Planting in the different location cultivation twice in 1 year, contracting using spring and winter in different regional breedings The short breeding time limit, saves manpower and material resources, has saved breeding cost and accelerated breeding process.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in the embodiment of the present invention Technical solution is clearly and completely described, it is clear that described embodiments are some of the embodiments of the present invention, rather than complete The embodiment in portion.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts Every other embodiment obtained, shall fall within the protection scope of the present invention.
Musky gourd includes long tubular and oblate spheroid etc., wherein long tubular musky gourd is since trait expression is long tubular, Its fruit kind chamber is larger, and the seed quantity of general fruit is more;And oblate spheroid musky gourd due to trait expression be oblate spheroid, Fruit kind chamber is smaller, and the seed quantity of fruit is relatively fewer, but seed is big, i.e., plate is wide and plate length is all larger.The present invention is with length As material B and without climing oblate spheroid in climing long tubular musky gourd homozygosis material or long climing oblate spheroid musky gourd homozygosis material Shape musky gourd homozygosis materials A, obtains material of the target without climing seed-use pumpkin, the character of performance be without climing, seed quantity it is more or Person's seed is big.
It is described in detail below by two embodiments.
Embodiment 1:Without excellent No. 1 breeding of musky gourd material 1292 and an aromatic plant metioned in ancient books of climing oblate spheroid seed
1, winter in 2005 will be without climing oblate spheroid musky gourd homozygosis material 1092 and long climing oblate spheroid in Sanya, Hainan base Shape musky gourd homozygosis material 1066 hybridizes;Climing 1066 trait expression of oblate spheroid musky gourd homozygosis material of the length is that seed plate is long 2.5cm, plate width are 1.3cm;
2、F1It is plant without climing, fruit in Hefei ,Anhui base Planted in spring in 2006 is that oblate spheroid, seed are big It is small between 1092 and 1066, single fruit seed 350, use 1066 to be returned as recurrent parent and primary obtain BC1
3、BC1It is separated in winter in 2006 in Sanya, Hainan base Planted, then with recurrent parent 1066 and all BC1 The single plant backcrossing of plantation obtains BC2, choose without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm single fruit of plate;
4、BC2Separation is still showed in the plantation of Hefei ,Anhui base in spring in 2007, then with recurrent parent 1066 and is owned BC2The single plant backcrossing of plantation obtains BC3,
5, winter in 2007 in Sanya, Hainan base to plant without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm of plate BC3Single plant is selfed, and BC is obtained3F2
6, it chooses without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate3F2Single plant, and respectively at the spring in 2008 Season, winter in 2008, spring in 2009, winter in 2009 were continuously selfed for 4 generations, continuously preferably most finally BC3F6In generation, obtains homozygosis certainly Based material is handed over, preferred standard is to choose without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm of plate.BC3F6Trait expression For plant without climing, fruit oblate spheroid, the yellowish-white skin of mellow fruit, fruit mercolized wax powder be light, in pulp yellowish red color, meat coarse powder, quality on, it is single Fruit seed grain number 300, the long 2.5cm of plate, the wide 1.3cm of plate, 100-grain weight 35g.Mean fruit weight 2kg.Now determine the homozygosis based material to compile Number be 1292.
7, spring in 2011 is in Hefei ,Anhui base, long climing musky gourd homozygosis material 1053,1053 trait expression For length is climing, oblate spheroid, single fruit kernal number 450, long climing musky gourd homozygosis material 1053 carries out miscellaneous with homozygosis based material 1292 Hand over, winter in 2011 Sanya, Hainan base performance plant without climing, mellow fruit oblate spheroid, fruit colour of loess pattern, fruit mercolized wax powder compared with Weight, pulp yellow, meat coarse powder, quality are preferable, single fruit kernal number 370, the long 2.1cm of plate, the wide 1.1cm of plate, 100-grain weight 24g. Single fruit weight 3kg or so, easily bears fruit, and no tendril is good, convenient dense planting.Now name is an aromatic plant metioned in ancient books excellent No. 1.
Embodiment 2:Without climing long tubular musky gourd material 1295 and excellent No. 2 breedings of an aromatic plant metioned in ancient books
1, spring in 2010 will be without climing oblate spheroid musky gourd homozygosis material 1092 and long climing long cylinder in Hefei ,Anhui base Shape musky gourd homozygosis material 1095 hybridizes;The trait expression of the climing long tubular musky gourd homozygosis material 1095 of the length is single fruit kind Seed number 520;
2、F1It is plant in Sanya, Hainan base Planted in winter in 2010 without climing, fruit is flat pyriform, with 1095 Primary acquisition BC is returned as recurrent parent1
3、BC1It plants and separates in Hefei ,Anhui base in spring in 2011, to BC1It is all selfed, then uses recurrent parent 1095 and all BC1The single plant backcrossing of plantation obtains BC2, choose the BC of single fruit seed number 4502Single plant is selfed, and is obtained BC2F2
4, the BC without climing, long tubular, single fruit seed number 450 is chosen2F2Single plant, respectively at winter in 2011,2012 Spring, winter in 2012, spring in 2013, winter in 2013, spring in 2014, winter in 2014 were continuously selfed for 7 generations, continuously It is preferred that single plant of the plant without the long tubular of climing, fruit, single fruit kernal number 450 or more, most finally BC2F9In generation, obtains homozygous inbred lines Material, trait expression be plant without in the long tubular of climing, fruit, mellow fruit safflower skin, fruit mercolized wax powder, pulp it is deeply orange red, Meat is thin, quality is preferable, single fruit kernal number 520, the long 1.7cm of plate, the wide 0.9cm of plate, 100-grain weight 16g.Mean fruit weight 2.8kg.Now determining the homozygous line material number is 1295.
5, spring in 2015 is in Hefei ,Anhui base, long climing musky gourd homozygosis material 1066,1066 trait expression For length is climing, oblate spheroid, the long 2.5cm of seed plate, the wide 1.3cm of plate;Long climing musky gourd homozygosis material 1066 and homozygous based material 1295 are hybridized, and winter in 2015 plants experimentally in Sanya, Hainan base, show as plant without climing, consistency is good, mellow fruit high ball Shape, fruit color colour of loess skin, fruit mercolized wax powder weight.Pulp is orange red, quality fine powder, quality are more excellent.Single fruit kernal number 450, plate are long The wide 1cm of 2cm, plate, 100-grain weight 22g.Single fruit weight 3kg or so, easily bears fruit, and no tendril is good, convenient dense planting.It now names as an aromatic plant metioned in ancient books excellent 2 Number.
It should be noted that is be related in above-described embodiment is climing without climing oblate spheroid musky gourd homozygosis material 1092, length Oblate spheroid musky gourd homozygosis material 1066, homozygous line material number are 1292, grow climing musky gourd homozygosis material 1053, nothing Climing oblate spheroid musky gourd homozygosis material 1092, long climing long tubular musky gourd homozygosis material 1095, homozygous line material number are 1295, only inventor in order toBetter illustrate the specific embodiment that technical solution is enumerated, wherein 1092,1066, 1292,1053,1095,1295 etc. number represent only biomaterials code name, not represent specific kind, the number and The essence of technical solution is unrelated.Institute of the present invention is realized in determination that can be beyond all doubt according to above-mentioned record those skilled in the art Necessary biomaterial is not the biomaterial that the digital code represents, but identical as the digital code biomaterial performance The biomaterial of character.As long as therefore meeting the character of above-mentioned needs, this programme can be realized.
In conclusion the embodiment of the present invention compared with the prior art, has the advantages that:
1, the embodiment of the present invention uses long for seed plate without climing oblate spheroid musky gourd homozygosis materials A and trait expression 2.5cm or more, the climing musky gourd homozygosis material B of length that plate width is 1.3cm or more or single fruit kernal number 520 or more, with parent This B is continuously preferably returned as recurrent parent, selfing obtains BCn+1Fm+1, choose without climing, the long 2.2~3cm of seed plate, plate are wide by 1.1 The BC of~1.5cm or single fruit seed number 450 or moren+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, pure Self-mating system material C is closed to hybridize to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;It provides in seed use State's pumpkin specific breed, this without climing seed with the merit of three kinds of parents of musky gourd set, have plant without climing, long Gesture is steady, female flower is more, easily bear fruit, single fruit kernal number 350 or more, and plate is long, plate is wide, 100-grain weight shows excellent, single fruit weight 3kg More than, yield is high, is conducive to dense planting, can bring considerable economic benefit for enterprise and peasant household.
2, the embodiment of the present invention is continuously preferred according to the otherness progress strange land of north and south region ecological condition.North and south region latitude Degree is different, and sunshine duration is different, selects the insensitive single plant of counterglow using continuous preferred mode.So that finally obtaining Without climing seed musky gourd to natural environment adaptability enhance, plantation region it is wider.
3, the embodiment of the present invention completes Planting in the different location training twice in 1 year using spring and winter in different regional breedings It educates, shortens the breeding time limit, save manpower and material resources, saved breeding cost and accelerated breeding process.
It should be noted that, in this document, the terms "include", "comprise" or its any other variant are intended to non-row His property includes, so that the process, method, article or equipment for including a series of elements not only includes those elements, and And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic Element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that including institute State in the process, method, article or equipment of element that there is also other identical elements.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments Invention is explained in detail, those skilled in the art should understand that:It still can be to aforementioned each implementation Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.

Claims (8)

1. a kind of high-quality without the climing seed selection of musky gourd kind, which is characterized in that include the following steps:
S1:It chooses without climing oblate spheroid musky gourd homozygosis materials A and long climing musky gourd homozygosis material B as parents, obtains To F1;
S2:It is returned the parent B as recurrent parent and the F1, obtains BC1
S3:BC1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or more BC1Single plant uses the parent B as recurrent parent continuous backcross, continuously preferably obtains BCn+1
S4:BCn+1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed Number 450 or more BCn+1Single plant is selfed, and BC is obtainedn+1Fm+1
S5:It chooses without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or moren+ 1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C;
S6:The homozygous inbred lines material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;
Wherein, n is natural number, and n≤1, m are natural number, and m≤1;
The trait expression of the climing musky gourd homozygosis material B and E of length is the long 2.5cm or more of seed plate, plate width is 1.3cm or more Or single fruit kernal number 520 or more.
2. according to claim 1 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:The length Climing musky gourd homozygosis material B is long climing long tubular musky gourd homozygosis material or long climing oblate spheroid musky gourd homozygosis material One of;The climing musky gourd homozygosis material E of length is long climing long tubular musky gourd homozygosis material or long climing oblate spheroid One of musky gourd homozygosis material.
3. according to claim 2 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:The length The trait expression of climing oblate spheroid musky gourd homozygosis material is oblate spheroid, the long 2.5cm or more of plate, the wide 1.3cm or more of plate, 100-grain weight 35g or more, single fruit kernal number 300 or more;
The trait expression of the climing long tubular musky gourd homozygosis material of length is long tubular, the long 1.7cm or more of plate, the wide 0.9cm of plate Above, 100-grain weight 16g or more, 520 or more single fruit kernal number.
4. according to claim 1 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:Step S1 In obtain F1Crossing system be A × B or B × A;Obtained in the step S6 be without the Crossing system of climing seed musky gourd C × E or E × C.
5. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists In:BC is obtained in step S3n+1Backcrossing mode be according to north and south region ecological condition otherness carry out strange land it is continuously preferred.
6. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists In:BC is obtained in step S4n+1Fm+1Selfing mode be according to north and south region ecological condition otherness carry out strange land it is continuously excellent Choosing.
7. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists In:The n is 1-8, m 1-10.
8. any according to claim 1~7 described high-quality educated with the selection of musky gourd kind in pumpkin without climing seed Application in kind.
CN201810770515.8A 2018-07-13 2018-07-13 High-quality selection and application without climing seed with musky gourd kind Pending CN108849477A (en)

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CN114107535A (en) * 2020-08-31 2022-03-01 北京市农林科学院 Molecular marker for identifying character of removing wax powder of grafted cucumber fruit from Chinese pumpkin rootstock and application of molecular marker
CN114107535B (en) * 2020-08-31 2023-08-18 北京市农林科学院 Molecular marker for identifying properties of removing wax powder of grafted cucumber fruits of pumpkin stock and application of molecular marker

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