CN108849477A - High-quality selection and application without climing seed with musky gourd kind - Google Patents
High-quality selection and application without climing seed with musky gourd kind Download PDFInfo
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- CN108849477A CN108849477A CN201810770515.8A CN201810770515A CN108849477A CN 108849477 A CN108849477 A CN 108849477A CN 201810770515 A CN201810770515 A CN 201810770515A CN 108849477 A CN108849477 A CN 108849477A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention provides a kind of high-quality selection and application without climing seed musky gourd kind, is related to crop breeding technical field.Use without climing oblate spheroid musky gourd homozygosis materials A and trait expression as the long 2.5cm or more of seed plate, plate width is 1.3cm or more or single fruit kernal number 520 or more climing musky gourd homozygosis material B of length, use parent B to be continuously preferably returned as recurrent parent, be selfed and obtain BCn+1Fm+1, choose without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or moren+ 1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, and homozygous inbred lines material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;Provide a seed musky gourd specific breed, the merit of three kinds of parents of the kind set, have plant without it is climing, single fruit kernal number 350 or more, plate is long, plate is wide, 100-grain weight show it is excellent, conducive to dense planting, considerable economic benefit can be brought for enterprise and peasant household.
Description
Technical field
The present invention relates to crop breeding technical fields, and in particular to a kind of high-quality without the choosing of musky gourd kind of climing seed
Educate method and application.
Background technique
Seed-use pumpkin is the traditional agricultural and sideline product in China and foreign trade commodity using seed as main edible organs.Pumpkin
Essential fatty acid, amino acid, phytosterol, minerals, vitamin and polysaccharide rich in etc., have drive away helminth,
The effects of reducing cholesterol, anti-inflammatory, anti-oxidant, alleviation hypertension, reduction bladder and urethra pressure.
Existing seed-use pumpkin can be divided into musky gourd, giant pumpkin, american pumpkin etc., product be referred to as flash,
Blank or snow-white, tabula rasa.Seed-use pumpkin is long in China's developing history, and especially in nearly development in 30 years, seed-use pumpkin is produced
Industry forms the industrial system of " breeding, production, processing, popularization, marketing integration ".
But existing more seed-use pumpkin kind, mainly giant pumpkin (blank) and America are applied in production
Pumpkin (tabula rasa), musky gourd (flash) there is no seed specific breed at present, and the breeding for being engaged in this respect specific breed does not appear in the newspapers
Road.It is available without specific breed in production at present, cause the enterprise's raw material quality for being engaged in the processing of the type pumpkin irregular,
The product quality fluctuation processed is big, seriously affects enterprise's health, orderly development.Therefore, the excellent seed China of Breeding Traits
Pumpkin specific breed increases economic results in society, promotes the development of China's pumpkin industrialization to have non-for meeting market supply
Often important meaning.
Summary of the invention
(1) the technical issues of solving
In view of the shortcomings of the prior art, the present invention provides a kind of high-quality selection without climing seed musky gourd kind and answers
With solving the technical issues of seed musky gourd is without specific breed in the prior art.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs:
It is a kind of high-quality without the climing seed selection of musky gourd kind, include the following steps:
S1:It chooses miscellaneous as parent without climing oblate spheroid musky gourd homozygosis materials A and long climing musky gourd homozygosis material B
It hands over, obtains F1;
S2:It is returned the parent B as recurrent parent and the F1, obtains BC1;
S3:BC1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit
Seed number 450 or more BC1Single plant uses the parent B as recurrent parent continuous backcross, continuously preferably obtains BCn+1;
S4:BCn+1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit
Seed number 450 or more BCn+1Single plant is selfed, and BC is obtainedn+1Fm+1;
S5:It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or more
BCn+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C;
S6:The homozygous inbred lines material C hybridizes to obtain without climing seed South China with long climing musky gourd homozygosis material E
Melon;
Wherein, n is natural number, and n≤1, m are natural number, and m≤1;
The trait expression of the climing musky gourd homozygosis material B and E of length is the long 2.5cm or more of seed plate, plate width is
1.3cm or more or single fruit kernal number 520 or more.
Preferably, the climing musky gourd homozygosis material B of the length is that long climing long tubular musky gourd homozygosis material or length are climing
One of oblate spheroid musky gourd homozygosis material;The climing musky gourd homozygosis material E of length is long climing long tubular musky gourd
One of homozygous material or long climing oblate spheroid musky gourd homozygosis material.
Preferably, the trait expression of the climing oblate spheroid musky gourd homozygosis material of the length be oblate spheroid, the long 2.5cm of plate with
Upper, the wide 1.3cm or more of plate, 100-grain weight 35g or more, single fruit kernal number 300 or more;
The trait expression of the climing long tubular musky gourd homozygosis material of length is that long tubular, the long 1.7cm or more of plate, plate are wide
0.9cm or more, 100-grain weight 16g or more, 520 or more single fruit kernal number.
Preferably, F is obtained in step S11Crossing system be A × B or B × A;It obtains in the step S6 without climing seed
It is C × E or E × C with the Crossing system of musky gourd.
Preferably, BC is obtained in step S3n+1Backcrossing mode be according to north and south region ecological condition otherness carry out it is different
Ground is continuously preferred.
Preferably, BC is obtained in step S4n+1Fm+1Selfing mode be according to north and south region ecological condition otherness into
Row strange land is continuously preferred.
Preferably, the n is 1-8, m 1-10.
It high-quality is educated with the selection of musky gourd kind in pumpkin without climing seed the present invention also provides a kind of using above-mentioned
Application in kind.
(3) beneficial effect
The present invention provides a kind of high-quality selection and application without climing seed musky gourd kind, compared with prior art
Advantage is:
1, the present invention use without climing oblate spheroid musky gourd homozygosis materials A and trait expression for the long 2.5cm or more of seed plate,
Plate width is the climing musky gourd homozygosis material B of length of 1.3cm or more or single fruit kernal number 520 or more, uses parent B as circulation
Parent is continuously preferably returned, selfing obtains BCn+1Fm+1, choose without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or
The BC of single fruit seed number 450 or moren+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, homozygous inbred lines material
Material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;One seed musky gourd special product is provided
Kind, this without climing seed with the merit of three kinds of parents of musky gourd set, have plant without climing, growing way is steady, female flower
It is more, easily bear fruit, single fruit kernal number 350 or more, plate is long, plate is wide, 100-grain weight shows excellent, single fruit weight 3kg or more, yield
Height is conducive to dense planting, can bring considerable economic benefit for enterprise and peasant household.
2, the present invention is continuously preferred according to the otherness progress strange land of north and south region ecological condition.North and south region latitude is not
Together, sunshine duration is different, selects the insensitive single plant of counterglow using continuous preferred mode.So that finally obtained nothing
Climing seed musky gourd enhances natural environment adaptability, and plantation region is wider.
3, the present invention completes Planting in the different location cultivation twice in 1 year, contracting using spring and winter in different regional breedings
The short breeding time limit, saves manpower and material resources, has saved breeding cost and accelerated breeding process.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in the embodiment of the present invention
Technical solution is clearly and completely described, it is clear that described embodiments are some of the embodiments of the present invention, rather than complete
The embodiment in portion.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, shall fall within the protection scope of the present invention.
Musky gourd includes long tubular and oblate spheroid etc., wherein long tubular musky gourd is since trait expression is long tubular,
Its fruit kind chamber is larger, and the seed quantity of general fruit is more;And oblate spheroid musky gourd due to trait expression be oblate spheroid,
Fruit kind chamber is smaller, and the seed quantity of fruit is relatively fewer, but seed is big, i.e., plate is wide and plate length is all larger.The present invention is with length
As material B and without climing oblate spheroid in climing long tubular musky gourd homozygosis material or long climing oblate spheroid musky gourd homozygosis material
Shape musky gourd homozygosis materials A, obtains material of the target without climing seed-use pumpkin, the character of performance be without climing, seed quantity it is more or
Person's seed is big.
It is described in detail below by two embodiments.
Embodiment 1:Without excellent No. 1 breeding of musky gourd material 1292 and an aromatic plant metioned in ancient books of climing oblate spheroid seed
1, winter in 2005 will be without climing oblate spheroid musky gourd homozygosis material 1092 and long climing oblate spheroid in Sanya, Hainan base
Shape musky gourd homozygosis material 1066 hybridizes;Climing 1066 trait expression of oblate spheroid musky gourd homozygosis material of the length is that seed plate is long
2.5cm, plate width are 1.3cm;
2、F1It is plant without climing, fruit in Hefei ,Anhui base Planted in spring in 2006 is that oblate spheroid, seed are big
It is small between 1092 and 1066, single fruit seed 350, use 1066 to be returned as recurrent parent and primary obtain BC1;
3、BC1It is separated in winter in 2006 in Sanya, Hainan base Planted, then with recurrent parent 1066 and all BC1
The single plant backcrossing of plantation obtains BC2, choose without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm single fruit of plate;
4、BC2Separation is still showed in the plantation of Hefei ,Anhui base in spring in 2007, then with recurrent parent 1066 and is owned
BC2The single plant backcrossing of plantation obtains BC3,
5, winter in 2007 in Sanya, Hainan base to plant without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm of plate
BC3Single plant is selfed, and BC is obtained3F2;
6, it chooses without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate3F2Single plant, and respectively at the spring in 2008
Season, winter in 2008, spring in 2009, winter in 2009 were continuously selfed for 4 generations, continuously preferably most finally BC3F6In generation, obtains homozygosis certainly
Based material is handed over, preferred standard is to choose without climing, seed plate long 2.2~3cm, the wide 1.1~1.5cm of plate.BC3F6Trait expression
For plant without climing, fruit oblate spheroid, the yellowish-white skin of mellow fruit, fruit mercolized wax powder be light, in pulp yellowish red color, meat coarse powder, quality on, it is single
Fruit seed grain number 300, the long 2.5cm of plate, the wide 1.3cm of plate, 100-grain weight 35g.Mean fruit weight 2kg.Now determine the homozygosis based material to compile
Number be 1292.
7, spring in 2011 is in Hefei ,Anhui base, long climing musky gourd homozygosis material 1053,1053 trait expression
For length is climing, oblate spheroid, single fruit kernal number 450, long climing musky gourd homozygosis material 1053 carries out miscellaneous with homozygosis based material 1292
Hand over, winter in 2011 Sanya, Hainan base performance plant without climing, mellow fruit oblate spheroid, fruit colour of loess pattern, fruit mercolized wax powder compared with
Weight, pulp yellow, meat coarse powder, quality are preferable, single fruit kernal number 370, the long 2.1cm of plate, the wide 1.1cm of plate, 100-grain weight 24g.
Single fruit weight 3kg or so, easily bears fruit, and no tendril is good, convenient dense planting.Now name is an aromatic plant metioned in ancient books excellent No. 1.
Embodiment 2:Without climing long tubular musky gourd material 1295 and excellent No. 2 breedings of an aromatic plant metioned in ancient books
1, spring in 2010 will be without climing oblate spheroid musky gourd homozygosis material 1092 and long climing long cylinder in Hefei ,Anhui base
Shape musky gourd homozygosis material 1095 hybridizes;The trait expression of the climing long tubular musky gourd homozygosis material 1095 of the length is single fruit kind
Seed number 520;
2、F1It is plant in Sanya, Hainan base Planted in winter in 2010 without climing, fruit is flat pyriform, with 1095
Primary acquisition BC is returned as recurrent parent1;
3、BC1It plants and separates in Hefei ,Anhui base in spring in 2011, to BC1It is all selfed, then uses recurrent parent
1095 and all BC1The single plant backcrossing of plantation obtains BC2, choose the BC of single fruit seed number 4502Single plant is selfed, and is obtained
BC2F2;
4, the BC without climing, long tubular, single fruit seed number 450 is chosen2F2Single plant, respectively at winter in 2011,2012
Spring, winter in 2012, spring in 2013, winter in 2013, spring in 2014, winter in 2014 were continuously selfed for 7 generations, continuously
It is preferred that single plant of the plant without the long tubular of climing, fruit, single fruit kernal number 450 or more, most finally BC2F9In generation, obtains homozygous inbred lines
Material, trait expression be plant without in the long tubular of climing, fruit, mellow fruit safflower skin, fruit mercolized wax powder, pulp it is deeply orange red,
Meat is thin, quality is preferable, single fruit kernal number 520, the long 1.7cm of plate, the wide 0.9cm of plate, 100-grain weight 16g.Mean fruit weight
2.8kg.Now determining the homozygous line material number is 1295.
5, spring in 2015 is in Hefei ,Anhui base, long climing musky gourd homozygosis material 1066,1066 trait expression
For length is climing, oblate spheroid, the long 2.5cm of seed plate, the wide 1.3cm of plate;Long climing musky gourd homozygosis material 1066 and homozygous based material
1295 are hybridized, and winter in 2015 plants experimentally in Sanya, Hainan base, show as plant without climing, consistency is good, mellow fruit high ball
Shape, fruit color colour of loess skin, fruit mercolized wax powder weight.Pulp is orange red, quality fine powder, quality are more excellent.Single fruit kernal number 450, plate are long
The wide 1cm of 2cm, plate, 100-grain weight 22g.Single fruit weight 3kg or so, easily bears fruit, and no tendril is good, convenient dense planting.It now names as an aromatic plant metioned in ancient books excellent 2
Number.
It should be noted that is be related in above-described embodiment is climing without climing oblate spheroid musky gourd homozygosis material 1092, length
Oblate spheroid musky gourd homozygosis material 1066, homozygous line material number are 1292, grow climing musky gourd homozygosis material 1053, nothing
Climing oblate spheroid musky gourd homozygosis material 1092, long climing long tubular musky gourd homozygosis material 1095, homozygous line material number are
1295, only inventor in order toBetter illustrate the specific embodiment that technical solution is enumerated, wherein 1092,1066, 1292,1053,1095,1295 etc. number represent only biomaterials code name, not represent specific kind, the number and The essence of technical solution is unrelated.Institute of the present invention is realized in determination that can be beyond all doubt according to above-mentioned record those skilled in the art Necessary biomaterial is not the biomaterial that the digital code represents, but identical as the digital code biomaterial performance The biomaterial of character.As long as therefore meeting the character of above-mentioned needs, this programme can be realized.
In conclusion the embodiment of the present invention compared with the prior art, has the advantages that:
1, the embodiment of the present invention uses long for seed plate without climing oblate spheroid musky gourd homozygosis materials A and trait expression
2.5cm or more, the climing musky gourd homozygosis material B of length that plate width is 1.3cm or more or single fruit kernal number 520 or more, with parent
This B is continuously preferably returned as recurrent parent, selfing obtains BCn+1Fm+1, choose without climing, the long 2.2~3cm of seed plate, plate are wide by 1.1
The BC of~1.5cm or single fruit seed number 450 or moren+1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C, pure
Self-mating system material C is closed to hybridize to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;It provides in seed use
State's pumpkin specific breed, this without climing seed with the merit of three kinds of parents of musky gourd set, have plant without climing, long
Gesture is steady, female flower is more, easily bear fruit, single fruit kernal number 350 or more, and plate is long, plate is wide, 100-grain weight shows excellent, single fruit weight 3kg
More than, yield is high, is conducive to dense planting, can bring considerable economic benefit for enterprise and peasant household.
2, the embodiment of the present invention is continuously preferred according to the otherness progress strange land of north and south region ecological condition.North and south region latitude
Degree is different, and sunshine duration is different, selects the insensitive single plant of counterglow using continuous preferred mode.So that finally obtaining
Without climing seed musky gourd to natural environment adaptability enhance, plantation region it is wider.
3, the embodiment of the present invention completes Planting in the different location training twice in 1 year using spring and winter in different regional breedings
It educates, shortens the breeding time limit, save manpower and material resources, saved breeding cost and accelerated breeding process.
It should be noted that, in this document, the terms "include", "comprise" or its any other variant are intended to non-row
His property includes, so that the process, method, article or equipment for including a series of elements not only includes those elements, and
And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic
Element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that including institute
State in the process, method, article or equipment of element that there is also other identical elements.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that:It still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (8)
1. a kind of high-quality without the climing seed selection of musky gourd kind, which is characterized in that include the following steps:
S1:It chooses without climing oblate spheroid musky gourd homozygosis materials A and long climing musky gourd homozygosis material B as parents, obtains
To F1;
S2:It is returned the parent B as recurrent parent and the F1, obtains BC1;
S3:BC1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number
450 or more BC1Single plant uses the parent B as recurrent parent continuous backcross, continuously preferably obtains BCn+1;
S4:BCn+1Trait expression separation;It chooses without climing, the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed
Number 450 or more BCn+1Single plant is selfed, and BC is obtainedn+1Fm+1;
S5:It chooses without climing, the BC of the long 2.2~3cm of seed plate, the wide 1.1~1.5cm of plate or single fruit seed number 450 or moren+ 1Fm+1Single plant carries out continuous selfing and obtains homozygous inbred lines material C;
S6:The homozygous inbred lines material C hybridizes to obtain without climing seed musky gourd with long climing musky gourd homozygosis material E;
Wherein, n is natural number, and n≤1, m are natural number, and m≤1;
The trait expression of the climing musky gourd homozygosis material B and E of length is the long 2.5cm or more of seed plate, plate width is 1.3cm or more
Or single fruit kernal number 520 or more.
2. according to claim 1 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:The length
Climing musky gourd homozygosis material B is long climing long tubular musky gourd homozygosis material or long climing oblate spheroid musky gourd homozygosis material
One of;The climing musky gourd homozygosis material E of length is long climing long tubular musky gourd homozygosis material or long climing oblate spheroid
One of musky gourd homozygosis material.
3. according to claim 2 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:The length
The trait expression of climing oblate spheroid musky gourd homozygosis material is oblate spheroid, the long 2.5cm or more of plate, the wide 1.3cm or more of plate, 100-grain weight
35g or more, single fruit kernal number 300 or more;
The trait expression of the climing long tubular musky gourd homozygosis material of length is long tubular, the long 1.7cm or more of plate, the wide 0.9cm of plate
Above, 100-grain weight 16g or more, 520 or more single fruit kernal number.
4. according to claim 1 high-quality without the climing seed selection of musky gourd kind, it is characterised in that:Step S1
In obtain F1Crossing system be A × B or B × A;Obtained in the step S6 be without the Crossing system of climing seed musky gourd
C × E or E × C.
5. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists
In:BC is obtained in step S3n+1Backcrossing mode be according to north and south region ecological condition otherness carry out strange land it is continuously preferred.
6. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists
In:BC is obtained in step S4n+1Fm+1Selfing mode be according to north and south region ecological condition otherness carry out strange land it is continuously excellent
Choosing.
7. according to any one of claims 1 to 4 high-quality without the climing seed selection of musky gourd kind, feature exists
In:The n is 1-8, m 1-10.
8. any according to claim 1~7 described high-quality educated with the selection of musky gourd kind in pumpkin without climing seed
Application in kind.
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Cited By (1)
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CN114107535A (en) * | 2020-08-31 | 2022-03-01 | 北京市农林科学院 | Molecular marker for identifying character of removing wax powder of grafted cucumber fruit from Chinese pumpkin rootstock and application of molecular marker |
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CN114107535B (en) * | 2020-08-31 | 2023-08-18 | 北京市农林科学院 | Molecular marker for identifying properties of removing wax powder of grafted cucumber fruits of pumpkin stock and application of molecular marker |
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