CN106171961A - Selection and application without climing honey this type Fructus Cucurbitae moschatae - Google Patents
Selection and application without climing honey this type Fructus Cucurbitae moschatae Download PDFInfo
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- CN106171961A CN106171961A CN201610591543.4A CN201610591543A CN106171961A CN 106171961 A CN106171961 A CN 106171961A CN 201610591543 A CN201610591543 A CN 201610591543A CN 106171961 A CN106171961 A CN 106171961A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention provides a kind of selection without climing honey this type Fructus Cucurbitae moschatae and application, relates to crop breeding, technical field of plant protection.Comprise the following steps: 1, choose non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys as parents, obtain F1；2, using parent B as recurrent parent and described F1Continuous backcross n time, the most preferably obtains BCn, then by BCnIn selfing m generation continuously, the most preferably obtain homozygous inbred lines material BCnFm+1；3, by BCnFm+1Isozygoty material C hybridization with elongated musky gourd, obtain trait expression for without climing honey this type Fructus Cucurbitae moschatae.The present invention solves honey this type Fructus Cucurbitae moschatae in prior art and is unfavorable for the technical problem of dense planting.The present invention has precocity without climing honey this type Fructus Cucurbitae moschatae；The long rod of fruit, in good taste；Being beneficial to dense planting without climing kind, yield is high；Strange land is carried out the most preferred so that Local Adaptation is strong, it is possible to bring considerable economic benefit for enterprise and peasant household according to the diversity of region, north and south ecological condition.
Description
Technical field
The present invention relates to crop breeding, technical field of plant protection, be specifically related to a kind of without climing honey this type Fructus Cucurbitae moschatae
Selection and application.
Background technology
Fructus Cucurbitae moschatae is Cucurbitaceae Cucurbita annual crop.The kind the most generally cultivated is a lot, mainly has three
Cultivar: i.e. musky gourd (being commonly called as pumpkin), american pumpkin (being commonly called as Cucurbita pepo L.), giant pumpkin (being commonly called as Cucurbita maxima).Existing
In musky gourd kind, planting more is honey this type Fructus Cucurbitae moschatae.Expanding bottom honey this type pumpkin fruit, melon body is the longest, closely
Like Fructus Chaenomelis shape, aging fruit yellow, there is light yellow piebaldism.Its sarcocarp is fine and closely woven sweet glutinous, and quality is splendid, and has strong resistance to virosis.
Although honey this type Fructus Cucurbitae moschatae has above-mentioned plurality of advantages, but, honey this type Fructus Cucurbitae moschatae is climing property herbaceous plant, its
Long climing characteristic, is unfavorable for dense planting, is the major reason being difficult to high yield；And there is the shortcoming that counterglow is sensitive, planting site
Territory is confined to Yangtze river basin areas to the south.
Therefore the precocity of an applicable market demand, high yield, high-quality, this class of honey that disease-resistant, Local Adaptation is strong are selected
Type Fructus Cucurbitae moschatae new varieties, produce development vegetable, meet market supply, increase economic results in society, promote China's Fructus Cucurbitae moschatae industrialization
Development have very important meaning.
Summary of the invention
(1) solve the technical problem that
Not enough for prior art, the present invention provides a kind of selection without climing honey this type Fructus Cucurbitae moschatae and application, solves
In prior art, honey this type Fructus Cucurbitae moschatae is unfavorable for the technical problem of dense planting.
(2) technical scheme
For realizing object above, the present invention is achieved by the following technical programs:
A kind of selection without climing honey this type Fructus Cucurbitae moschatae, comprises the following steps:
1) choose non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys as parents, obtain F1, institute
State F1Trait expression is without climing flat pyriform；
2) using described parent B as recurrent parent and described F1Continuous backcross n time, the most preferably obtains BCn, then by described
BCnIn selfing m generation continuously, the most preferably obtain homozygous inbred lines material BCnFm+1；
Described BCnShow as without climing and that other characteristic is identical with parent B character；
Described BCnFm+1Show as through isozygoty without climing and that other characteristic is identical with parent B character；
3), by described homozygous inbred lines material BCnFm+1With elongated musky gourd isozygoty material C hybridization, obtain trait expression
For without climing honey this type Fructus Cucurbitae moschatae；
Wherein, n is natural number, and n 1, and m is natural number, and m 1.
Preferably, described non-trailing pumpkin isozygoty materials A for without climing oblate spheroid musky gourd isozygoty material 1092, without climing oblate spheroid
Shape musky gourd isozygotys material 1128 and isozygotying the one in material 1130 without climing long-neck cylindrical shape musky gourd.
Preferably, described elongated musky gourd isozygoty material B for long climing long-neck cylindrical shape musky gourd isozygoty material 1076,
The one in material 1133 that long climing long tubular musky gourd isozygotys material 1171 and long climing length curved cylindrical shape musky gourd isozygotys.
Preferably, described elongated musky gourd material C of isozygotying is isozygotied material 1192, long climing for long climing long tubular musky gourd
The one in material 1171 that long curved cylindrical shape musky gourd isozygotys material 1133 and long climing long tubular musky gourd isozygotys.
Preferably, step 1) in obtain F1Crossing system be A × B or B × A.
Preferably, step 3) in obtain the Crossing system without climing honey this type Fructus Cucurbitae moschatae be BCnFm+1× C or C × BCnFm+1。
Preferably, step 2) middle acquisition BCnThe mode that backcrosses be to carry out different according to the diversity of region, north and south ecological condition
Ground is the most preferably.
Preferably, step 2) middle acquisition BCnFm+1Selfing mode be to carry out according to the diversity of region, north and south ecological condition
Strange land is the most preferred.
Preferably, described n be 1-8, m be 1-10.
Present invention also offers a kind of utilize the above-mentioned selection without climing honey this type Fructus Cucurbitae moschatae in Fructus Cucurbitae moschatae breeding should
With.
(3) beneficial effect
The present invention provides a kind of selection without climing honey this type Fructus Cucurbitae moschatae and application, and compared with prior art advantage exists
In:
1, the present invention use non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys as parents, with parent
This B goes out as recurrent parent continuous backcross, selfed breeding and isozygotys material BC without climing elongated musky gourdnFm+1, then with elongated China
The Fructus Cucurbitae moschatae material C cross breeding that isozygotys is without climing honey this type pumpkin variety, and this is without climing honey this type three kinds of parents' of pumpkin variety collection
Merit, have precocity, plant without climing, growing way is sane, female flower is many, easily bear fruit, fruit is elongated, meat is tight, matter
Ground fine powder, in good taste and yield high, it is beneficial to dense planting without climing kind, it is possible to bring considerable economic benefit for enterprise and peasant household.
2, to carry out strange land according to the diversity of region, north and south ecological condition the most preferred for the present invention.Region, north and south latitude is not
With, sunshine, duration was different, used the most preferably mode to select the individual plant that counterglow is insensitive.And then make the nothing finally given
Climing honey this type Fructus Cucurbitae moschatae adapts to ability to natural environment and strengthens, and plantation region is not limited solely to Yangtze river basin areas to the south, is growing
Northern area, basin, river can be planted equally, and shows precocity, high yield, high-quality, feature disease-resistant, adaptable equally.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
Fig. 1 is that the embodiment of the present invention 1 is preced with the selection-breeding stream of No. a kind without climing long-neck cylindrical shape musky gourd material 1130 and an aromatic plant metioned in ancient books
Cheng Tu；
Fig. 2 is that the embodiment of the present invention 2 is preced with the selection-breeding flow process of No. 2 kinds without climing long tubular musky gourd material 1235 and an aromatic plant metioned in ancient books
Figure；
Fig. 3 is that the embodiment of the present invention 3 is preced with the selection-breeding stream of No. 3 kinds without climing length curved cylindrical shape musky gourd material 1298 and an aromatic plant metioned in ancient books
Cheng Tu.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under not making creative work premise, broadly falls into the scope of protection of the invention.
As described in background technology, honey this type Fructus Cucurbitae moschatae involved in the embodiment of the present invention is cenospecies, fruit
Bottom is expanded, and melon body is the longest, approximates Fructus Chaenomelis shape, aging fruit yellow, has light yellow piebaldism；Its sarcocarp is fine and closely woven sweet glutinous, and quality is splendid,
And virosis is had strong resistance.Although honey this type Fructus Cucurbitae moschatae has above-mentioned plurality of advantages, but, honey this type Fructus Cucurbitae moschatae is climing property
Herbaceous plant, its long climing characteristic, it is unfavorable for dense planting, is the major reason being difficult to high yield；And there is counterglow sensitivity
Shortcoming, plantation region is confined to Yangtze river basin areas to the south.
Also have at present a class non-trailing pumpkin, this non-trailing pumpkin to belong to early seasonal strain in the northern area of China, fruit oblate spheroid, red
Flower skin, because of without climing, so beneficially dense planting, and without geographical restrictions, but, the type non-trailing pumpkin kind mouthfeel is poor.
On the advantage basis of above two Fructus Cucurbitae moschatae, embodiments provide a kind of without climing honey this type Fructus Cucurbitae moschatae
Selection, comprises the following steps:
1) choose non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys as parents, obtain F1, institute
State F1Trait expression is without climing flat pyriform；
2) using described parent B as recurrent parent and described F1Continuous backcross n time, the most preferably obtains BCn, then by described
BCnIn selfing m generation continuously, the most preferably obtain homozygous inbred lines material BCnFm+1；
Described BCnShow as without climing and that other characteristic is identical with parent B character；
Described BCnFm+1Show as through isozygoty without climing and that other characteristic is identical with parent B character；
3), by described homozygous inbred lines material BCnFm+1With elongated musky gourd isozygoty material C hybridization, obtain trait expression
For without climing honey this type Fructus Cucurbitae moschatae；
Wherein, n is natural number, and n 1, and m is natural number, and m 1.
The embodiment of the present invention by the way, uses isozygoty materials A and elongated musky gourd of non-trailing pumpkin to isozygoty material B
As parents, go out as recurrent parent continuous backcross, selfed breeding with parent B and isozygoty material without climing elongated musky gourd
BCnFm+1, then with the elongated musky gourd material C cross breeding that isozygotys without climing honey this type pumpkin variety, this is without climing this type of honey south
Melon kind integrates the merit of three kinds of parents, have precocity, plant without climing, growing way is sane, female flower is many, easily bear fruit, really
True length shape, meat closely, quality fine powder, in good taste and yield high, be beneficial to dense planting without climing kind, it is possible to for enterprise and peasant household
Bring considerable economic benefit.
As the preferred mode of one, described non-trailing pumpkin isozygotys materials A for isozygotying material without climing oblate spheroid musky gourd
1092, isozygoty material 1128 and isozygotying in material 1130 without climing long-neck cylindrical shape musky gourd without climing oblate spheroid musky gourd
Kind.
As the preferred mode of one, described elongated musky gourd isozygotys material B for long climing long-neck cylindrical shape musky gourd
Isozygoty material 1076, long climing long tubular musky gourd isozygotys material 1171 and long climing length curved cylindrical shape musky gourd isozygotys material
One in 1133.
As the preferred mode of one, described elongated musky gourd material C of isozygotying is isozygotied for long climing long tubular musky gourd
Material 1192, long climing length curved cylindrical shape musky gourd isozygoty material 1133 and long climing long tubular musky gourd isozygotys in material 1171
One.
As the preferred mode of one, step 1) in obtain F1Crossing system be A × B or B × A.
As the preferred mode of one, step 3) in obtain the Crossing system without climing honey this type Fructus Cucurbitae moschatae be BCnFm+1×C
Or C × BCnFm+1。
As the preferred mode of one, step 2) middle acquisition BCnThe mode that backcrosses be according to region, north and south ecological condition
It is the most preferred that diversity carries out strange land.
As the preferred mode of one, step 2) middle acquisition BCnFm+1Selfing mode be according to region, north and south ecological condition
Diversity to carry out strange land the most preferred.
As the preferred mode of one, described n be 1-8, m be 1-10.
The embodiment of the present invention passes through above-mentioned optimal way, carries out strange land according to the diversity of region, north and south ecological condition continuous
Preferably.Region, north and south latitude is different, and sunshine, duration was different, uses the most preferably mode to select the individual plant that counterglow is insensitive.
And then making finally give without climing honey this type Fructus Cucurbitae moschatae, natural environment to be adapted to ability enhancing, plantation region is not limited solely to long
Basin, river areas to the south, can plant equally in northern area, the Yangtze river basin, and shows precocity, high yield, high-quality equally, resists
Feature sick, adaptable.
It addition, the embodiment of the present invention additionally provides and a kind of utilizes the above-mentioned selection without climing honey this type Fructus Cucurbitae moschatae at Fructus Cucurbitae moschatae
Application in breeding.
It should be noted that the non-trailing pumpkin materials A of isozygotying of educating in above-described embodiment (includes without climing oblate spheroid musky gourd
Isozygoty material 1092, isozygoty material 1128 without climing oblate spheroid musky gourd), the elongated musky gourd material B that isozygotys (include long climing length
Isozygoty material 1076, long climing long tubular musky gourd of neck cylindrical shape musky gourd isozygotys in material 1171 and the long curved cylindrical shape of climing length
State's Fructus Cucurbitae moschatae isozygotys material 1133) and elongated musky gourd material C of isozygotying (include that long climing long tubular musky gourd isozygotys material
1192, grow climing length curved cylindrical shape musky gourd and isozygoty material 1133 and long climing long tubular musky gourd isozygotys material 1171) every
Optional material model, is all and is commercially available from Anhui Quanyin Gaoke Seed Industry Co.ltd.
It is described in detail below by three embodiments.
Embodiment 1: as it is shown in figure 1, be preced with the selection-breeding of No. a kind without climing long-neck cylindrical shape musky gourd material 1130 and an aromatic plant metioned in ancient books
1, winter in 2005 will isozygoty material 1092 without climing oblate spheroid musky gourd in Sanya, Hainan base and grow climing long-neck
The cylindrical shape musky gourd material 1076 that isozygotys hybridizes；
2、F1In spring in 2006 base, Hefei ,Anhui Planted be plant be flat pyriform without climing, fruit, with 1076
Backcross as recurrent parent and once obtain BC1；
3、BC1Separate at Sanya, Hainan base Planted winter in 2006, then with recurrent parent 1076 and without climing, long
Neck cylindrical shape individual plant backcrosses and obtains BC2；
4、BC2Spring in 2007, in base, Hefei ,Anhui, plantation still showed separation, then with recurrent parent 1076 and without climing,
Long-neck cylindrical shape individual plant backcrosses and obtains BC3；
5, winter in 2007 in Sanya, Hainan base to BC3In carry out selfing, preferred fructo without climing, long-neck cylindrical shape individual plant
Shape is excellent, annesl good, yellowish pink individual plant deep, that quality is excellent obtains BC3F2；
6, again respectively at spring in 2008, winter in 2008, spring in 2009, continuous selfing 4 generation winter in 2009, continuously
The most finally BC3F6In generation, obtains homozygous inbred lines material.Its trait expression is that plant is without climing, fruit long-neck cylindrical shape, mellow fruit
Folium Lantanae Camarae skin, really mercolized wax grain weight, sarcocarp are orange red, meat fine powder, excellent quality, mean fruit weight 2.5kg.Now determine this homozygous line
Material number is 1130.
7, spring in 2011 is in base, Hefei ,Anhui, trial combination 1130 (without climing, long-neck cylindrical shape) × 1192 (long climing,
Long tubular, big fruit), winter in 2011 Sanya, Hainan base performance plant without climing, the long rod of mellow fruit, the fruit colour of loess red,
Really mercolized wax powder is heavier, and sarcocarp is orange red, meat fine powder, quality are excellent, single fruit weight about 3.5kg, easily bears fruit, good without climing property, suitableeer
Preferably dense planting.Now name and be preced with No. 1 into an aromatic plant metioned in ancient books.
Embodiment 2: as in figure 2 it is shown, be preced with the selection-breeding of No. 2 kinds without climing long tubular musky gourd material 1235 and an aromatic plant metioned in ancient books
1, spring in 2010 will isozygoty material 1128 without climing oblate spheroid musky gourd in base, Hefei ,Anhui and grow climing long cylinder
The shape musky gourd material 1171 that isozygotys hybridizes；
2、F1In winter in 2010 Sanya, Hainan base Planted be plant be flat pyriform without climing, fruit, with 1171
Backcross as recurrent parent and once obtain BC1；
3, spring in 2011, in base, Hefei ,Anhui, plantation separated, to BC BC 11In carry out without tubular individual plant climing, long
Selfing, it is thus achieved that BC1F2；
4, in winter in 2011, spring in 2012, winter in 2012, spring in 2013, winter in 2013, spring in 2014
Season, continuous selfing 7 generation winter in 2014, continuously preferably plant without climing, the long tubular of fruit, really shape is excellent, annesl good, yellowish pink deep, product
Individual plant of fine quality, the most finally BC1F9In generation, obtains homozygous inbred lines material, and its trait expression is that plant is without climing, the long tubular of fruit, one-tenth
Ripe fruit Folium Lantanae Camarae skin, really mercolized wax grain weight, sarcocarp is the most orange red, meat fine powder, quality are excellent, mean fruit weight 3.5kg.Now determine this pure
Syzygy material number is 1235.
5, spring in 2015 is in base, Hefei ,Anhui, and trial combination 1235 (without tubular climing, long) × 1133 are (long climing, long curved
Cylindrical shape, big fruit), winter in 2015 plants experimentally in Sanya, Hainan base, show as plant without climing, concordance good, mellow fruit length is straight
Pear-Shaped, really color Folium Lantanae Camarae skin, really mercolized wax grain weight, really shape is good, is difficult to deformity.Sarcocarp is the most orange red, quality carefully tight powder, quality
Excellent.Single fruit weight about 4.5kg, Comprehensive Traits is more excellent.Now name and be preced with No. 2 into an aromatic plant metioned in ancient books.
Embodiment 3: be preced with the selection-breeding of No. 3 kinds without climing length curved cylindrical shape musky gourd material 1298 and an aromatic plant metioned in ancient books
1, winter in 2010 will be climing with long without the climing long-neck cylindrical shape musky gourd material 1130 that isozygotys in Sanya, Hainan base
The long curved cylindrical shape musky gourd material 1133 that isozygotys hybridizes；
F1In spring in 2011 base, Hefei ,Anhui Planted be plant be long-neck cylindrical shape without climing, fruit, use
1133 backcross as recurrent parent once obtains BC1；
BC1In winter in 2011 Sanya, Hainan base Planted separate, then with recurrent parent 1133 with without climing, grow curved
Cylindrical shape individual plant backcrosses and obtains BC2；
Take to also require that selection continuous backcross 3 generation, BC spring in 2012, winter in 2012, spring in 2013 again5In
Winter in 2013, plantation was still presented with separating a little, to BC in Sanya, Hainan base5In carry out without curved cylindrical shape individual plant climing, long
Selfing, preferred fructo shape is excellent, annesl good, yellowish pink individual plant deep, that quality is excellent obtains BC5F2；
Again respectively at spring in 2014, continuous selfing 2 generation winter in 2014, BC the most finally5F4Generation obtains isozygotys
Selfing line material, its trait expression is that plant is without climing, the curved cylindrical shape of fruit length, mellow fruit colour of loess Folium Styracis Suberifoliae, really mercolized wax grain weight, sarcocarp
The most orange red, meat fine powder, quality are excellent, mean fruit weight 3.5kg.Now determining this homozygous line material number is 1298.
6, spring in 2015 is in base, Hefei ,Anhui, trial combination 1298 (without curved cylindrical shape climing, long, in big fruit) × 1171
(long tubular climing, long, high-quality), winter in 2015 plants experimentally in Sanya, Hainan base, performance plant without climing, strong in growing way, resistance,
Easily cultivation.The straight rod of mellow fruit length, fruit Folium Lantanae Camarae skin, really mercolized wax grain weight, sarcocarp is the most orange red, quality is the sweetest, and opaque is high, product
Of fine quality, single fruit weight about 4kg, general performance is excellent.Now name and be preced with No. 3 into an aromatic plant metioned in ancient books.
In sum, the embodiment of the present invention compared with prior art possesses following beneficial effect:
1, the embodiment of the present invention uses non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys miscellaneous as parent
Hand over, go out as recurrent parent continuous backcross, selfed breeding with parent B and isozygoty material BC without climing elongated musky gourdnFm+1, then with
The elongated musky gourd material C cross breeding that isozygotys is without climing honey this type pumpkin variety, and this is without climing honey this type pumpkin variety collection three
The merit of kind of parent, have precocity, plant without climing, growing way is sane, female flower is many, easily bear fruit, fruit is elongated, meat
Closely, quality fine powder, in good taste and yield high, be beneficial to dense planting without climing kind, it is possible to bring considerable warp for enterprise and peasant household
Ji benefit.
2, to carry out strange land according to the diversity of region, north and south ecological condition the most preferred for the embodiment of the present invention.Region, north and south latitude
Degree difference, sunshine, duration was different, used the most preferably mode to select the individual plant that counterglow is insensitive.And then make to finally give
Without climing honey this type Fructus Cucurbitae moschatae to natural environment adapt to ability strengthen, plantation region be not limited solely to Yangtze river basin areas to the south,
Can plant equally in northern area, the Yangtze river basin, and show precocity, high yield, high-quality, spy disease-resistant, adaptable equally
Point.
3, the embodiment of the present invention utilizes spring and winter in different regional breedings, completes 1 year twice Planting in the different location training
Educate, shorten the breeding time limit, save manpower and materials, saved breeding cost and accelerated breeding process.
It should be noted that in this article, term " includes ", " comprising " or its any other variant are intended to non-row
Comprising of his property, so that include that the process of a series of key element, method, article or equipment not only include those key elements, and
And also include other key elements being not expressly set out, or also include intrinsic for this process, method, article or equipment
Key element.In the case of there is no more restriction, statement " including ... " key element limited, it is not excluded that including
State and the process of key element, method, article or equipment there is also other identical element.
Above example only in order to technical scheme to be described, is not intended to limit；Although with reference to previous embodiment
The present invention is described in detail, it will be understood by those within the art that: it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or wherein portion of techniques feature is carried out equivalent；And these amendment or
Replace, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (10)
1. the selection without climing honey this type Fructus Cucurbitae moschatae, it is characterised in that comprise the following steps:
1) choose non-trailing pumpkin materials A and the elongated musky gourd material B that isozygotys that isozygotys as parents, obtain F1, described F1Property
Shape shows as without climing flat pyriform；
2) using described parent B as recurrent parent and described F1Continuous backcross n time, the most preferably obtains BCn, then by described BCnEven
In continuous selfing m generation, the most preferably obtain homozygous inbred lines material BCnFm+1；Described BCnShow as without climing and other characteristic and described parent
The character that this B is identical；Described BCnFm+1Show as through isozygoty without climing and other characteristic and described character identical for parent B；
3), by described homozygous inbred lines material BCnFm+1With elongated musky gourd isozygoty material C hybridization, obtaining trait expression is nothing
Climing honey this type Fructus Cucurbitae moschatae；
Wherein, n is natural number, and n 1, and m is natural number, and m 1.
Selection without climing honey this type Fructus Cucurbitae moschatae the most according to claim 1, it is characterised in that: described non-trailing pumpkin is pure
Condensation material A for without climing oblate spheroid musky gourd isozygoty material 1092, isozygoty material 1128 and without climing without climing oblate spheroid musky gourd
Long-neck cylindrical shape musky gourd isozygotys the one in material 1130.
Selection without climing honey this type Fructus Cucurbitae moschatae the most according to claim 1, it is characterised in that: described elongated South China
The melon material B that isozygotys isozygotys material for isozygoty material 1076, long climing long tubular musky gourd of long climing long-neck cylindrical shape musky gourd
1171 and long climing length curved cylindrical shape musky gourd isozygoty the one in material 1133.
Selection without climing honey this type Fructus Cucurbitae moschatae the most according to claim 1, it is characterised in that: described elongated South China
Melon material C of isozygotying is isozygotied material for isozygoty material 1192, long climing length curved cylindrical shape musky gourd of long climing long tubular musky gourd
1133 and long climing long tubular musky gourd isozygoty the one in material 1171.
5. according to the described selection without climing honey this type Fructus Cucurbitae moschatae arbitrary in Claims 1 to 4, it is characterised in that: step
1) F is obtained in1Crossing system be A × B or B × A.
6. according to the described selection without climing honey this type Fructus Cucurbitae moschatae arbitrary in Claims 1 to 4, it is characterised in that: step
3) obtaining the Crossing system without climing honey this type Fructus Cucurbitae moschatae in is BCnFm+1× C or C × BCnFm+1。
7. according to the described selection without climing honey this type Fructus Cucurbitae moschatae arbitrary in Claims 1 to 4, it is characterised in that: step
2) BC is obtained innThe mode that backcrosses be that to carry out strange land according to the diversity of region, north and south ecological condition the most preferred.
8. according to the described selection without climing honey this type Fructus Cucurbitae moschatae arbitrary in Claims 1 to 4, it is characterised in that: step
2) BC is obtained innFm+1Selfing mode be that to carry out strange land according to the diversity of region, north and south ecological condition the most preferred.
9. according to the described selection without climing honey this type Fructus Cucurbitae moschatae arbitrary in Claims 1 to 4, it is characterised in that: described n
It is 1-10 for 1-8, m.
10. one kind utilizes in claim 1～9 arbitrary described selection without climing honey this type Fructus Cucurbitae moschatae in Fructus Cucurbitae moschatae breeding
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| CN106962175A (en) * | 2017-03-15 | 2017-07-21 | 湖南省蔬菜研究所 | The method for creating of carpopodium impact resistance and the excellent Pumpkin Germplasm resource of Comprehensive Traits |
| CN107950386A (en) * | 2017-12-29 | 2018-04-24 | 浙江大学 | The method for creating of pumpkin interspecific hybridization recombinant inbred lines |
| CN108849477A (en) * | 2018-07-13 | 2018-11-23 | 安徽荃银高科瓜菜种子有限公司 | High-quality selection and application without climing seed with musky gourd kind |
| CN113785770A (en) * | 2021-09-27 | 2021-12-14 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106962175A (en) * | 2017-03-15 | 2017-07-21 | 湖南省蔬菜研究所 | The method for creating of carpopodium impact resistance and the excellent Pumpkin Germplasm resource of Comprehensive Traits |
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| CN107950386A (en) * | 2017-12-29 | 2018-04-24 | 浙江大学 | The method for creating of pumpkin interspecific hybridization recombinant inbred lines |
| CN108849477A (en) * | 2018-07-13 | 2018-11-23 | 安徽荃银高科瓜菜种子有限公司 | High-quality selection and application without climing seed with musky gourd kind |
| CN113785770A (en) * | 2021-09-27 | 2021-12-14 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
| CN113785770B (en) * | 2021-09-27 | 2022-07-29 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
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