CN108841392A - Utilize the conditioner of microorganism remediation organic polluted soil - Google Patents

Abstract

The present invention utilizes microorganisms to repair the conditioner of organic polluted soil, and the mass percentage of its components is: 5-20% of microbial flora fermentation liquid; 20-50% of organic improver; 30-75% of culture medium; said microbial flora It involves 19 genera including Acinetobacter, Micrococcus, Bacillus, Candida, Trichoderma, and Aspergillus; the organic amendments are diatomaceous earth, humus, straw, One or more of biochar, sawdust, bagasse, and plant ash; the culture medium contains potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, limestone and soybean meal, and the conditioner of the present invention is conducive to degrading organic pollutants The growth and reproduction of the microbial flora can adjust the pH of the soil and the nutrient ratio of carbon, nitrogen and phosphorus, effectively improve the composition and structure of the organically polluted soil, and improve the condition of decreased ventilation caused by soil compaction, thereby effectively repairing the contaminated soil. organically polluted soil.

Description

A conditioner for remediating organically polluted soil using microorganisms

technical field

The invention belongs to the technical field of using microorganisms to repair polluted soil, in particular to a conditioner for using microorganisms to restore organic pollution, especially diesel-polluted soil.

Background technique

Soil is one of the necessary natural resources for human beings, animals, plants and microorganisms to survive. Land does not necessarily have soil, and soil is closely related to human survival (farming and animal husbandry). Originally there was a dynamic balance of matter and energy between the soil and the environment, but with the strengthening and impact of human activities, the dynamic balance between the soil and the environment has gradually changed: the soil is gradually being polluted, among which organic pollution (Petroleum and its products) have increasingly serious impacts and harms on soil and the environment, and polluted soil has restricted and seriously affected the survival of humans, animals, plants, and microorganisms. At present, the leakage of diesel oil during transportation and use constitutes a major pollution to the soil. Due to the special physical and chemical properties of diesel oil with low density, strong adhesion and low emulsification ability, it is difficult to remove after entering the soil, and it remains in the soil for a long time, which greatly increases the carbon source in the soil, thus destroying the original The ratio of carbon, nitrogen, and phosphorus can easily compact the soil, change the pH, and destroy the structure and composition of the soil, which will have a great negative impact on the ecological environment, crop production, and human survival in polluted areas. Therefore, remediation of diesel-contaminated soil is an important work involving human survival. At present, the methods for remediating organically polluted soil mainly include physical chemical remediation and biological remediation. The bioremediation method also includes microbial remediation and phytoremediation. Among them, microbial remediation is widely used because of its advantages of low cost, high efficiency and no secondary pollution.

In the microbial remediation method, measures such as adding an external conditioner (high-efficiency degrading bacteria), adding an appropriate amount of straw, and deep plowing (turning) the soil are generally adopted. Judging from the current actual effect, there are still some deficiencies in the existing microbial remediation methods, which mainly include: (1) The existing conditioners will affect the indigenous flora and form an antagonistic effect, which is not conducive to the remediation of contaminated soil. (2) Adding an appropriate amount of straw can relieve soil compaction and enhance ventilation to a certain extent, but it has little effect on soil polluted by diesel oil. (3) Deep plowing (turning) the soil is not only a complicated process, but also time-consuming and labor-intensive, making the operation difficult. The emergence of these unfavorable factors limits the application of microbial remediation in the remediation of diesel-contaminated soil, or in other words, affects the effectiveness of remediation of diesel-contaminated soil. Therefore, it is very necessary to improve the existing microbial remediation method.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a conditioner that utilizes microorganisms to remediate organically polluted soil, which can effectively improve the composition and structure of organic (petroleum and its products) polluted soil, and adjust the pH and carbon content of the soil. The nutrient ratio of nitrogen and phosphorus can improve the situation of decreased ventilation caused by soil compaction. At the same time, it can add indigenous microbial flora to improve the activity of microorganisms, thereby improving the efficiency of using microorganisms to repair organically polluted soil.

The focus of the present invention is to construct an efficient degrading bacteria group. At present, one of the keys to remediation of contaminated soil is to find efficient degrading bacteria groups that can interact with each other, so that they can play a role in complex and organically polluted soils, and transform organic pollutants in soil through microbial metabolic processes. into non-toxic fatty acids, carbon dioxide and water.

In order to achieve the above object, the present invention adopts the following technical solutions.

A conditioner utilizing microorganisms to remediate organically polluted soil is characterized in that it contains microbial flora, an organic amendment and a culture medium, and the mass percentage of the conditioner component is:

Microbial flora fermentation broth (OD=1.5～3) 5～20%;

Organic modifier 20-50%;

Medium 30-75%.

Further, the microbial flora is Acinetobacter, Micrococcus, Bacillus, Actinomycetes, Pseudomonas, Rhodococcus (Rhodococcus), Coryneforms, Enterococcus faecalis, Staphylococcus, Flavobacterium, Achromobacter, Lysinibacillus sphaericus), Arthrobacter, Nocardia, Candida, Trichos, Trichoderma, Aspergillus, white rot Fungi (write-rot fungi).

Furthermore, the organic modifier is one or more of diatomaceous earth, humus, straw, biochar, sawdust, bagasse, and plant ash.

Further, the medium is potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, limestone and soybean meal, and their mass percentages in the components are:

Further, the potassium dihydrogen phosphate and the dipotassium hydrogen phosphate are used as a phosphorus source, and the ammonium sulfate is used as a nitrogen source, and the ratio of carbon in the soil: nitrogen: phosphorus (C:N:P) is adjusted to 100～ 120:10～12:1.

A conditioner utilizing microorganisms to remediate organic polluted soil is characterized in that it contains microbial flora, an organic amendment and a culture medium, and said microbial flora includes Acinetobacter (Acinetobacter), Micrococcus (Micrococcus), spore Bacillus, Actinomycetes, Pseudomonas, Rhodococcus, Coryneforms, Enterococcus faecalis, Staphylococcus ( Staphylococcus), Flavobacterium, Achromobacter, Lysinibacillus sphaericus, Arthrobacter, Nocardia, Candida ( Candida), Trichos, Trichoderma, Aspergillus, and write-rot fungi; Dipotassium hydrogen, ammonium sulfate, limestone and soybean meal; the mass percentage of described conditioning agent component is:

Microbial flora fermentation broth 5-20%;

Organic modifier 20-50%;

Medium 30-75%.

Further, the mass percentages of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, limestone and soybean meal accounting for the components are respectively:

The present invention utilizes the active effect of the conditioner of microorganism repairing organic polluted soil to be:

(1) The microbial flora used in the present invention is to combine and optimize the microorganisms of different genera. The microorganisms of different genera belong to the common microorganisms in the soil, so there is no problem of antagonism with the indigenous microorganisms It can effectively degrade organic pollutants through the good synergy between different genera of microorganisms. It is not only suitable for soils with low concentrations of organic pollutants, but also can repair soils with high concentrations and compound concentrations of organic pollutants. effect.

(2) The organic amendments used are one or more of diatomite, humus, straw, biochar, sawdust, bagasse, and plant ash. They have both trace elements and a small amount of energy for soil microbial growth, and at the same time It can also loosen the soil and relieve the compaction of the soil.

(3) adopt the culture medium that contains potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate; Because a large amount of carbon sources have been accumulated in the soil of organic pollution, therefore, conditioner of the present invention combines potassium dihydrogen phosphate and hydrogen phosphate Dipotassium is used as a phosphorus source, and ammonium sulfate is used as a nitrogen source. By adding appropriate potassium dihydrogen phosphate, dipotassium hydrogen phosphate and ammonium sulfate, the ratio of carbon: nitrogen: phosphorus in the soil can be adjusted to 100-120:10-12: 1. This ratio is conducive to the growth and reproduction of the microbial flora.

(4) The culture medium of the present invention contains limestone, which is used as a regulator of soil pH and can keep the soil neutral or slightly alkaline, thereby facilitating the growth and reproduction of the microbial flora, while also being able to Improve soil compaction, facilitate soil aeration and provide sufficient oxygen.

(5) The culture medium of the present invention contains soybean meal, which can provide one or more biosurfactants, which can emulsify organic pollutants in the soil that are difficult to dissolve in water, reduce its surface tension, and increase microbial growth. Interfacial tension between groups and organic pollutants. The biosurfactant provided by soybean meal is friendly to the environment and will not cause secondary pollution.

(6) Conditioning agent of the present invention is conducive to the growth and reproduction of microbial flora capable of degrading organic pollutants, can regulate the pH of soil and the nutrient ratio of carbon, nitrogen and phosphorus, and effectively improve the composition and structure of organically polluted soil, Improve the condition of decreased ventilation caused by soil compaction, so as to effectively restore organically polluted soil.

Description of drawings

Fig. 1 is the change of pH after the conditioner of the present invention is added to soil polluted by diesel oil in application example 1 of the present invention.

Fig. 2 is a schematic diagram of the effect of remediation of organically polluted soil in Application Example 1 of the present invention.

Detailed ways

The specific implementation of the conditioner for remediating organic polluted soil by using microorganisms of the present invention is given below, providing 6 examples and 1 application example. However, it should be noted that implementation of the present invention is not limited to the following embodiments. When the conditioning agent of the present invention is used in practice, it should be properly adjusted according to the degree of pollution of the soil, the pH of the soil, and the difference in nutrients in the soil.

Example 1

(1) A conditioner utilizing microorganisms to remediate organically polluted soil, containing microbial flora, organic amendments and culture medium, said microbial flora being Acinetobacter (Acinetobacter), Micrococcus (Micrococcus), Bacillus A combination of five species of genus Bacillus, pseudomonas and Achromobacter; the organic modifier is a combination of diatomaceous earth, plant ash, humus, straw, and sawdust; the The medium is a combination of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, limestone and soybean meal.

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=1.5) 5%;

50% organic modifier, including: 20% diatomaceous earth, 10% plant ash, 10% humus, 5% straw, and 5% sawdust;

Medium 45%, including: potassium dihydrogen phosphate 12%, dipotassium hydrogen phosphate 8%, ammonium sulfate 19%, limestone 5%, soybean meal 1%.

(2) The components of the microbial flora, the organic amendment and the mass percentage of the medium are prepared by a conventional method to obtain the conditioner of the present invention utilizing microorganisms to remediate organically polluted soil, and the conditioner should be granules or powders status.

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil

The conditioners of the present invention can be used in both in situ and ex situ treatments.

Larger objects that hinder soil plowing should be removed before use, and sufficient water should be provided.

Sprinkle the conditioning agent of the present invention into organically polluted soil, and the moisture content of the soil is optimal at 20-50%.

Example 2

(1) A conditioner utilizing microorganisms to remediate organically polluted soil, containing microbial flora, organic amendments and culture medium, said microbial flora being Actinomycetes (Actinomycetes), Pseudomonas (pseudomonas), The combination of Rhodococcus (Rhodococcus), Coryneforms (Coryneforms), and Candida (Candida); the organic amendment is a combination of diatomaceous earth, plant ash, humus, bagasse and sawdust; The medium contained monopotassium phosphate, dipotassium phosphate, ammonium sulfate, limestone and soybean meal.

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=2.0) 10%;

45% organic modifier, including 15% diatomaceous earth, 15% plant ash, 7% humus, 5% bagasse, and 3% sawdust;

45% medium, including 10% potassium dihydrogen phosphate, 5% dipotassium hydrogen phosphate, 13% ammonium sulfate, 15% limestone, and 2% soybean meal.

(2) The present invention utilizes the preparation of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

Example 3

(1) A conditioner that utilizes microorganisms to remediate organically polluted soil contains microbial flora, an organic amendment and a culture medium, and the microbial flora is Enterococcus faecalis (Enterococcus faecalis), Staphylococcus (Staphylococcus), yellow Bacillus (Flavobacterium), white rot fungi (write-rot fungi) four kinds of combinations; the organic amendment is a combination of diatomaceous earth, plant ash, biochar and sawdust; the medium contains potassium dihydrogen phosphate, phosphoric acid Dipotassium hydrogen, ammonium sulfate, limestone and soybean meal.

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=2.5) 15%;

30% organic modifier, including 17% diatomaceous earth, 2% plant ash, 10% biochar, and 1% sawdust;

Medium 55%, including 8% potassium dihydrogen phosphate, 2% dipotassium hydrogen phosphate, 1% ammonium sulfate, 40% limestone, and 4% soybean meal.

(2) The present invention utilizes the preparation of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

Example 4

A conditioner for utilizing microorganisms to remediate organically polluted soil, containing microbial flora, an organic amendment and a culture medium, said microbial flora being Lysinibacillus sphaericus, Arthrobacter, Norca A combination of five species of Nocardia, Trichoderma, and Aspergillus; the organic amendment is a combination of diatomaceous earth, humus, and straw; the medium contains phosphoric acid Potassium dihydrogen, dipotassium phosphate, ammonium sulfate, limestone and soybean meal.

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=3.0) 20%;

20% organic modifier, including 7% diatomaceous earth, 10% humus, and 3% straw;

Medium 60%, including 5% potassium dihydrogen phosphate, 11% dipotassium hydrogen phosphate, 9% ammonium sulfate, 30% limestone, and 5% soybean meal.

(2) The present invention utilizes the preparation of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

Example 5

A conditioner for utilizing microorganisms to remediate organically polluted soil, containing microbial flora, an organic amendment and a culture medium, said microbial flora being Lysinibacillus sphaericus, Rhodococcus, Arthrobacter A combination of three species of Arthrobacter; the organic modifier is a combination of diatomaceous earth, humus, and straw; the culture medium contains potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, limestone and soybean meal .

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=1.5) 5%;

20% organic modifier, including 5% diatomaceous earth, 12% humus, and 3% straw;

Medium 75%, including 15% potassium dihydrogen phosphate, 7% dipotassium hydrogen phosphate, 20% ammonium sulfate, 30% limestone, and 3% soybean meal.

(2) The present invention utilizes the preparation of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

Example 6

A conditioner for utilizing microorganisms to remediate organically polluted soil, containing microbial flora, organic amendments and culture medium, said microbial flora is white-rot fungi (write-rot fungi), pseudomonas (pseudomonas), red A combination of three species of Rhodococcus; the organic modifier is a combination of diatomaceous earth, humus, biochar, straw, and sawdust; the medium contains potassium dihydrogen phosphate, dipotassium hydrogen phosphate, Ammonium sulfate, limestone and soybean meal.

The mass percentage of described conditioner component is:

Microbial flora fermentation broth (OD=2.0) 20%;

50% organic modifier, including 15% diatomaceous earth, 20% humus, 10% biochar, 3% straw, and 2% sawdust;

30% medium, including 5% potassium dihydrogen phosphate, 4% dipotassium hydrogen phosphate, 10% ammonium sulfate, 10% limestone, and 1% soybean meal.

(2) The present invention utilizes the preparation of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

(3) The present invention utilizes the use of the conditioner of microorganism repairing organic polluted soil (same as embodiment 1).

Application Example 1

Apply the conditioner for remediating organic polluted soil with microorganisms provided in Example 2 of the present invention to remediate soil contaminated by diesel oil.

(1) Pre-treatment of soil: remove larger objects that hinder soil plowing, and set up sufficient water sources.

(2) Set application implementation area and control area: in order to facilitate the measurement, divide and clarify the repaired area: set 3 application implementation areas, each area is 1 square meter; 3 control areas without application implementation, Each area is also 1 square meter.

(3) Sampling to determine the physical and chemical properties of the polluted soil and the content of diesel in the soil.

(4) Carry out plowing, the plowing depth is 30 centimetres.

(5) Sprinkle the conditioning agent of the present invention into the soil polluted by diesel oil in 3 application implementation areas according to the concentration ratio of 3%, and the moisture content of the soil is optimal at 20-50%.

(6) Utilize the phosphorus source of potassium dihydrogen phosphate and dipotassium hydrogen phosphate, and utilize the nitrogen source of ammonium sulfate to adjust the ratio of carbon: nitrogen: phosphorus in the soil to between 100～120:10～12:1, and measure regularly Determination of pH and residual diesel content in soil.

See Table 1 for the measurement structure of the physical and chemical properties of soil contaminated by diesel oil in Application Example 1.

Table 1. Physicochemical properties of soil polluted by diesel oil in application example 1

From Table 1, we can see that the soil polluted by diesel oil is alkaline, and the proportion of nutrients in the soil is uncoordinated. Therefore, the soil polluted by diesel oil is not suitable for the growth of crops and the growth and metabolism of microbial flora.

See Figure 1 for the change in pH after the conditioner of the present invention is added to the soil polluted by diesel oil in Application Example 1.

From the change of pH in Figure 1, we can see that the pH of the experimental group added with soil conditioner is finally maintained at about 7, this is because the added conditioner has the effect of limestone, in addition, microbial flora is using diesel oil as carbon At the same time, it also produces acidic substances, which will lower the pH of the soil, which is conducive to the growth of microbial flora. In the control group, the pH of the soil hardly changed, so it can be explained that the conditioner for using microorganisms to remediate organic polluted soil provided by Example 2 of the present invention can play a role in regulating the pH of the soil.

The effect of remediation of diesel-contaminated soil in Application Example 1 is shown in Figure 2.

It can be seen from Figure 2 that with the extension of time, the content of diesel oil in the soil polluted by diesel oil decreased, and the degradation rate of diesel oil reached 72% on the 38th day of restoration, while a small part of diesel oil was degraded in the control group. However, its degradation rate is much slower than that of the experimental group, and the repair effect is very obvious. Therefore, it can be proved that adding the conditioner for remediating organic polluted soil by using microorganisms provided in Example 2 of the present invention is very effective for remediating soil contaminated by diesel oil.

The results of Application Example 1 show that the conditioner utilizing microorganisms to remediate organically polluted soil of the present invention can provide a good growth and metabolism environment for microbial flora, and can simultaneously improve the composition, structure and porosity of soil, and can effectively degrade Organic (diesel) pollutants.

Claims (9)

1. a kind of conditioner using microorganism remediation organic polluted soil, which is characterized in that contain microbial flora, You Jigai The mass percent of good dose and culture medium, the conditioner component is：

Microbial flora fermentation liquid 5~20%；

Organic amendment 20~50%；

Culture medium 30~75%.

2. the conditioner according to claim 1 using microorganism remediation organic polluted soil, which is characterized in that described micro- Biological flora is acinetobacter, Micrococcus, bacillus, actinomyces, pseudomonas, Rhod, bar bacterium Category, enterococcus faecalis category, staphylococcus, Flavobacterium, achromobacter, lysine bacillus, Arthrobacter, promise Cattell Pseudomonas, candida, Trichosporon, trichoderma, aspergillus, white-rot fungi.

3. the conditioner according to claim 1 using microorganism remediation organic polluted soil, which is characterized in that described to have Machine modifying agent is the one or more of diatomite, fertile soil, stalk, charcoal, sawdust, bagasse, plant ash.

4. the conditioner according to claim 1 using microorganism remediation organic polluted soil, which is characterized in that the training Supporting base is potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, lime stone and dregs of beans.

5. the conditioner according to claim 4 using microorganism remediation organic polluted soil, which is characterized in that the training Supporting the mass percent that base accounts for the component for potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, lime stone and dregs of beans is respectively：

6. the conditioner according to claim 5 using microorganism remediation organic polluted soil, which is characterized in that will be described Potassium dihydrogen phosphate and the dipotassium hydrogen phosphate are as phosphorus source, using the ammonium sulfate as nitrogen source, by carbon in soil:Nitrogen:The ratio of phosphorus Example is adjusted to 100~120:10~12:1.

7. the conditioner according to claim 1 using microorganism remediation organic polluted soil, which is characterized in that described micro- OD=1.5~3 of biological flora fermentation liquid.

8. a kind of conditioner using microorganism remediation organic polluted soil, which is characterized in that contain microbial flora, You Jigai Good dose and culture medium, the microbial flora include acinetobacter, Micrococcus, bacillus, actinomyces, false unit cell Pseudomonas, Rhod, Corynebacterium, enterococcus faecalis category, staphylococcus, Flavobacterium, achromobacter, lysine gemma bar Pseudomonas, Arthrobacter, Nocardia, candida, Trichosporon, trichoderma, aspergillus, white-rot fungi belong to more categories Combination；The organic amendment is one kind or several of diatomite, fertile soil, stalk, charcoal, sawdust, bagasse, plant ash Kind；The culture medium contains potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, lime stone and dregs of beans；The matter of the conditioner component Measuring percentage is：

Microbial flora fermentation liquid 5~20%；

Organic amendment 20~50%；

Culture medium 30~75%.

9. the conditioner according to claim 8 using microorganism remediation organic polluted soil, which is characterized in that the phosphorus The mass percent that acid dihydride potassium, dipotassium hydrogen phosphate, ammonium sulfate, lime stone and dregs of beans account for the component is respectively：