CN1785539A - In situ biological restoring method of petroleum polluted soil - Google Patents
In situ biological restoring method of petroleum polluted soil Download PDFInfo
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- CN1785539A CN1785539A CN 200510130674 CN200510130674A CN1785539A CN 1785539 A CN1785539 A CN 1785539A CN 200510130674 CN200510130674 CN 200510130674 CN 200510130674 A CN200510130674 A CN 200510130674A CN 1785539 A CN1785539 A CN 1785539A
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Abstract
An in-situ biologic repairing method for the soil polluted by petroleum features that after the fungus-bacteria mixed liquid, soil improver, organic fertilizer and inorganic nutrients are applied to the polluted soil, the soil is irrigated, ploughed, and covered by plastic film.
Description
Technical field
The present invention relates to adopt additive and various engineering means to strengthen the method for mixed microorganism preparation to the biology in situ remediation efficiency of oil-polluted soils.The biology in situ recovery technique that belongs to oil-polluted soils.
Background technology
The bioremediation technology of oil-polluted soils can be divided into biology in situ reparation and heterotopic biological reparation.Biology in situ renovation method is fit to the improvement of pollution in wide area, and having does not need mobile soil, low, the simple operation and other advantages of cost.Usually need to add microorganism formulation in position in the biological restoration process with efficient degradation petroleum hydrocarbon ability.The original position reparation occurs in the open soil ecosystem, the uncontrollability of environmental factors such as the inhomogeneity of soil texture, the complexity of the ecosystem and weather often causes the microorganism formulation growth metabolism situation of interpolation poor, degrading activity is subjected to the inhibition of indigenous microorganism, causes remediation efficiency reduction, cycle stretch-out.Therefore, exploitation high-effective microorganism microbial inoculum, exploitation can promote that the auxiliary agent of the growth metabolism of microbial inoculum in soil and exploitation correlation engineering enhancements are to apply the key of biology in situ renovation method.
Summary of the invention
At the problems referred to above, the invention provides the biology in situ renovation method of the oil-polluted soils that a kind of applicability is strong, degradation efficiency is high.This method adopts the composition of fungi with petroleum hydrocarbon degradation ability and bacterium as the mixed microorganism preparation, the mixed microorganism preparation of effective dose is added in the petroleum-polluted soil, add soil conditioner, microbial activity promoter, fertilizer and the inorganic nutrient substance of effective dose.Employing is irrigated, turning over further guarantees the optimum condition of repair process with the engineering measure of water conservation insulation.Specifically may further comprise the steps:
1) in petroleum-polluted soil, adds 0.1～1kg/m
2Liquid mixed microorganism preparation, to account for soil quality percentage be 1%～10% additive, account for soil quality percentage is 1～5% fertilizer and inorganic nutrient substance, making the elements in Soil ratio is C: N: P: K=90～120: 10～12: 1～2: 2～4; Described liquid mixed microorganism preparation is made up of fungi liquid culture and bacterial liquid culture, and the mixed proportion of fungi liquid culture and bacterial liquid culture is 1: 0.1～10; Described fungi liquid culture is the individual plant liquid culture of thorn little Ke Yinhan bacterium of spore or white-rot fungi Phanerochaete chrysosporium, or the mixture of two kinds of fungi liquid cultures, and the fungi content in the fungal cultures is 10～80mg dry weight/ml; The bacterial liquid culture is the individual plant liquid culture with bacterial isolates of petroleum hydrocarbon degradation ability, and bacterial content is 10
6～10
12Individual/ml; Described additive is cellulose and lignin natural biomass;
2) adopt water conservation insulation measure to cover the porous mulch film, and adopt and irrigate and the measure of turning over, keeping soil moisture content is 20～30%; Turn over the operation cycle be 1～4 the week 1 time.
In the technical scheme of the present invention, the fungi content in the described fungal cultures is preferably 20～60mg dry weight/ml; Bacterial content is preferably 10
8～10
12Individual/ml.When the fungi liquid culture was the mixture of thorn spore little Ke Yinhan bacteria liquid culture and white-rot fungi Phanerochaete chrysosporium liquid culture, the mixed proportion of thorn spore little Ke Yinhan bacteria liquid culture and white-rot fungi Phanerochaete chrysosporium liquid culture was 1: 0.8～1.
In the technical scheme of the present invention, the bacterium with petroleum hydrocarbon degradation ability is one or more in pseudomonas, Nocardia, Rhod, Micrococcus, Mycobacterium, corynebacterium or the bacillus.
The natural biomass of cellulose described in the present invention and lignin adopts wood chip, stalk, straw etc.This additive can improve the gas porosity of soil, promotes the oxygen transmission, helps fungi breeding fast in soil.
As mentioned above, the present invention adopts fungi-bacterial liquid mixed microorganism preparation, effect by various auxiliary agents and engineering enhancements keeps and the BA of promotion microorganism formulation in the contaminated soil system, can reach the effect of the microorganism formulation cooperative degradation of petroleum hydrocarbon pollutant in contaminated soil that is added.Fungi and bacterium that this mixed microorganism preparation will have the petroleum hydrocarbon degradation ability are mixed and made into microorganism formulation, it is strong to the low molecular weight hydrocarbons degradation capability fully to have fully utilized bacterium, but produce the biology availability that biosurfactant improves petroleum hydrocarbon, solubility mesostate to fungi can continue advantages such as degraded, and fungi produces the strong ectoenzyme of degradation capability, can the bigger pollutant of degrading texture complicated molecule amount, advantage such as good-extensibility in soil system, realized the ecology cooperation in soil and the complementation of metabolic pathway between fungi and the bacterium, can improve degradation capability petroleum hydrocarbon.Of the present inventionly be to provide a kind of fungi bacterium mixed microorganism preparation biology in situ metabolism growth, that be fit to commercial Application in contaminated soil that helps to repair implementing process, had broad application prospects.
Further describe the present invention by the following examples, but do not limit the present invention.
The specific embodiment
Microorganism with petroleum hydrocarbon degradation ability of the present invention can be by screening from the soil of oil pollution or obtaining from the mode that various countries DSMZ buys known petroleum hydrocarbon degradation bacterial strain.
In an embodiment, adopt EPA 3546 described microwave solvent extraction standard methods to determine total petroleum hydrocarbons content in the soil.
The fertilizer that uses among the embodiment is chicken manure, and inorganic nutrient substance is urea, Diammonium phosphate (DAP) and potassium sulfate.Concrete consumption sees that embodiment 1 and embodiment 2 are described.
Embodiment 1
Use thorn little Ke Yinhan bacterium 1 strain of spore and pseudomonad 1 strain to prepare liquid mixed microorganism preparation in the present embodiment, the preparation method is as follows:
At the volume that 100ml liquid potato culture is housed is the little Ke Yinhan bacterium of inoculation thorn spore in the 300ml triangular flask, at 28 ℃, cultivates 3 days under the 160rpm condition, collects culture, and fungi content is 20mg dry weight/ml.At the volume that 100ml LB fluid nutrient medium is housed is to inoculate pseudomonad in the 300ml triangular flask, at 30 ℃, cultivates 1 day under the 160rpm condition, collects culture, and bacterial content is 10
8Individual/ml.With the fungal cultures and the bacterial cultures that obtain is the liquid mixed microorganism preparation that mixes obtain in embodiment use at 10: 1 according to mixed proportion.
The additive that uses in the present embodiment is stalk, and fertilizer is a chicken manure, and inorganic nutrient substance is urea, Diammonium phosphate (DAP) and potassium sulfate.
The Polluted area that embodiment 1 administers leaks the crude oil pollution that causes for the five factory's oil pipelines that recover the oil.Aforesaid liquid mixed microorganism preparation consumption is 1kg/m
2It is 10% that soil, stalk addition account for soil quality percentage, and it is 5% that the chicken manure consumption accounts for soil quality percentage, and the consumption of urea, Diammonium phosphate (DAP) and potassium sulfate is can guarantee that the elements in Soil ratio is C: N: P: K=90: 10: 1: 2.Irrigation is about 20% to keep soil moisture content, and the frequency of turning over is per 1 week 1 time, and soil surface covers the porous mulch film.
Carry out simultaneously a pair ofly according to the facts testing.Divide one at same Polluted area and be used for control experiment, difference is not add liquid mixed microorganism preparation.
Through 147 days biology in situ reparation, the total petroleum hydrocarbons content degradation rate in the enforcement group in the soil reached 72.32%, and control group is 25.10%.The variation of processing procedure petroleum hydrocarbon degradation rate sees Table 1.
The treatment effect of table 1. embodiment 1
| Time (my god) | Control group degradation rate (%) | Enforcement group degradation rate (%) |
| 0 37 79 117 147 | 0.00 4.78 9.59 15.78 25.10 | 0.00 41.66 60.05 65.00 72.32 |
Embodiment 2
Use Phanerochaete chrysosporium 1 strain and 1 strain of red coccus to prepare liquid mixed microorganism preparation in the present embodiment, the preparation method is as follows:
At the volume that 100ml liquid potato culture is housed is to inoculate Phanerochaete chrysosporium in the 300ml triangular flask, at 30 ℃, cultivates 3 days under the 170rpm condition, collects culture, and fungi content is 60mg dry weight/ml.At the volume that 100ml LB fluid nutrient medium is housed is to inoculate pseudomonad in the 300ml triangular flask, at 30 ℃, cultivates 2 days under the 200rpm condition, collects culture, and bacterial content is 10
12Individual/ml.With the fungal cultures and the bacterial cultures that obtain is the liquid mixed microorganism preparation that mixes obtain in embodiment use at 1: 10 according to mixed proportion.
The additive that uses in the present embodiment is straw, and fertilizer is a chicken manure, and inorganic nutrient substance is urea, Diammonium phosphate (DAP) and potassium sulfate.
Difference from Example 1 is: the Polluted area that present embodiment is administered leaks the crude oil pollution that causes for the two factory's oil pipelines that recover the oil.Aforesaid liquid mixed microorganism preparation consumption is 0.1kg/m
2It is 1% that soil, stalk addition account for soil quality percentage, and it is 1% that the chicken manure consumption accounts for soil quality percentage, and the consumption of urea, Diammonium phosphate (DAP) and potassium sulfate is can guarantee that the elements in Soil ratio is C: N: P: K=120: 12: 2: 4.Irrigation is about 30% to keep soil moisture content, and the frequency of turning over is per 4 weeks 1 time, and soil surface covers the porous mulch film.
Carry out simultaneously a pair ofly according to the facts testing.Divide one at same Polluted area and be used for control experiment, difference is not add liquid mixed microorganism preparation.
Through 161 days biology in situ reparation, the total petroleum hydrocarbons content degradation rate in the enforcement group in the soil reached 60.10%, and control group is 27.75%.The variation of processing procedure petroleum hydrocarbon degradation rate sees Table 2.
The treatment effect of table 2. embodiment 2
| Time (my god) | Control group degradation rate (%) | Enforcement group degradation rate (%) |
| 0 37 79 117 161 | 0.00 8.15 13.66 24.93 27.75 | 0.00 31.55 40.45 48.32 60.10 |
The present invention is described with reference to more excellent embodiment, and the those skilled in the art who is familiar with foregoing detailed description can make many changes and replacement, and it all is included in scope of the present invention and the flesh and blood.
Claims (5)
1. the biology in situ renovation method of an oil-polluted soils, it is characterized in that: this method comprises the steps:
1) in petroleum-polluted soil, adds 0.1～1kg/m
2Liquid mixed microorganism preparation, to account for soil quality percentage be 1%～10% additive, account for soil quality percentage is 1～5% fertilizer and inorganic nutrient substance, making the elements in Soil ratio is C: N: P: K=90～120: 10～12: 1～2: 2～4; Described liquid mixed microorganism preparation is made up of fungi liquid culture and bacterial liquid culture, and the mixed proportion of fungi liquid culture and bacterial liquid culture is 1: 0.1～10; Described fungi liquid culture is the individual plant liquid culture of thorn little Ke Yinhan bacterium of spore or white-rot fungi Phanerochaete chrysosporium, or the mixture of two kinds of fungi liquid cultures, and the fungi content in the fungal cultures is 10～80mg dry weight/m1; The bacterial liquid culture is the liquid culture with bacterial isolates of petroleum hydrocarbon degradation ability, and bacterial content is 10
6～10
12Individual/ml; Described additive is cellulose and lignin natural biomass;
2) adopt water conservation insulation measure to cover the porous mulch film, and adopt and irrigate and the measure of turning over, keeping soil moisture content is 20～30%; Turn over the operation cycle be 1～4 the week 1 time.
2. according to the biology in situ renovation method of the described a kind of oil-polluted soils of claim 1, it is characterized in that: the fungi content in the described fungal cultures is 20～60mg dry weight/ml; Bacterial content is 10
8～10
12Individual/ml.
3. according to the biology in situ renovation method of the described a kind of oil-polluted soils of claim 1, it is characterized in that: when the fungi liquid culture was the mixture of thorn spore little Ke Yinhan bacteria liquid culture and white-rot fungi Phanerochaete chrysosporium liquid culture, the mixed proportion of thorn spore little Ke Yinhan bacteria liquid culture and white-rot fungi Phanerochaete chrysosporium liquid culture was 1: 0.8～1.
4. according to the biology in situ renovation method of claim 1, the described a kind of oil-polluted soils of 2 or 3 arbitrary claims, it is characterized in that: described bacterium with petroleum hydrocarbon degradation ability is one or more in pseudomonas, Nocardia, Rhod, Micrococcus, Mycobacterium, corynebacterium or the bacillus.
5. according to the biology in situ renovation method of claim 1, the described a kind of oil-polluted soils of 2 or 3 arbitrary claims, it is characterized in that: the natural biomass of described cellulose and lignin adopts wood chip, stalk or straw.
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| CN102430567A (en) * | 2011-11-03 | 2012-05-02 | 中国石油天然气股份有限公司 | Crude oil degrading agent in soil polluted by crude oil |
| CN101898198B (en) * | 2009-05-27 | 2012-09-05 | 中国石油天然气集团公司 | Method for remedying oil pollution by unitedly using air shatter crack and microorganism and device thereof |
| CN102726256A (en) * | 2011-04-07 | 2012-10-17 | 何松庆 | New application method of trichoderma biocontrol agent |
| CN104803721A (en) * | 2014-01-27 | 2015-07-29 | 中国石油天然气股份有限公司 | Biological composting processing method of oil-based drilling wastes |
| CN104941992A (en) * | 2015-06-26 | 2015-09-30 | 西安工程大学 | Oily soil degrading method based on joint of pseudomonas, bacillus and electric field |
| CN104941991A (en) * | 2015-06-26 | 2015-09-30 | 西安工程大学 | Oil-polluted soil degradation method based on combination of plesiomonas, pseudomonas and electric field |
| CN105170644A (en) * | 2015-10-30 | 2015-12-23 | 李磊 | Bioremediation method for petroleum-polluted soil |
| CN105385645A (en) * | 2015-12-30 | 2016-03-09 | 叶君芝 | Liquid preparation capable of degrading petroleum pollutants |
| CN105478460A (en) * | 2016-01-12 | 2016-04-13 | 山东佳星环保科技有限公司 | Bioremediation method of oil-contaminated soil |
| CN105921512A (en) * | 2016-05-11 | 2016-09-07 | 陕西师范大学 | Sequential biological enhancement treatment method for severe oil-contaminated soil or oil sludge |
| CN106077079A (en) * | 2016-08-20 | 2016-11-09 | 余敏敏 | A kind of oil-polluted soils restorative procedure |
| CN106391697A (en) * | 2016-10-26 | 2017-02-15 | 北京生态岛科技有限责任公司 | Method for in-situ repair of polluted soil |
| CN107695086A (en) * | 2017-09-20 | 2018-02-16 | 绍兴鑫广科技有限公司 | The depth restorative procedure of soil near chemical plant |
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| CN104941991A (en) * | 2015-06-26 | 2015-09-30 | 西安工程大学 | Oil-polluted soil degradation method based on combination of plesiomonas, pseudomonas and electric field |
| CN104941992B (en) * | 2015-06-26 | 2018-02-27 | 西安工程大学 | Based on pseudomonad, bacillus and the united petroleum-contaminated soil biodegrading process of electric field |
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| CN105170644B (en) * | 2015-10-30 | 2017-12-12 | 李磊 | A kind of biological renovation method of oil-polluted soils |
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