CN104941991A - Oil-polluted soil degradation method based on combination of plesiomonas, pseudomonas and electric field - Google Patents
Oil-polluted soil degradation method based on combination of plesiomonas, pseudomonas and electric field Download PDFInfo
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- CN104941991A CN104941991A CN201510366588.7A CN201510366588A CN104941991A CN 104941991 A CN104941991 A CN 104941991A CN 201510366588 A CN201510366588 A CN 201510366588A CN 104941991 A CN104941991 A CN 104941991A
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Abstract
The invention discloses an oil-polluted soil degradation method based on combination of plesiomonas, pseudomonas and an electric field. The method concretely comprises the following steps that 1, a bactericide for oil-polluted soil is prepared by utilizing plesiomonas and pseudomonas; 2, to-be-treated oil-polluted soil is weighed, pretreated, and filled into a repair box for the oil-polluted soil; and 3, the bactericide for the oil-polluted soil prepared in Step 1 is added to the oil-polluted soil in the repair box in Step 2, the electric field is applied to the oil-polluted soil in the repair box, and degradation on the oil-polluted soil is accomplished under combined action of the bactericide for oil-polluted soil and the electric field. he oil-polluted soil degradation method based on the combination of plesiomonas, pseudomonas and the electric field can treat the oil-polluted soil, so that the oil content in the oil-polluted soil is reduced effectively.
Description
Technical field
The invention belongs to petroleum-contaminated soil processing method technical field, be specifically related to a kind of petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad.
Background technology
At present, comparatively unmanageable in Solid Waste Treatment is exactly oily sludge, it is in oil extraction process, draw and to produce in the accidental leakage of the crude oil such as drilling well, operation, well workover, oil recovery, collection are defeated, accumulating and equipment control, its existence creates very large hidden danger to the ecological environment of periphery, must carry out harmless treatment to it.If the oils in oily sludge directly enters in soil, great pollution will be caused to soil.
Normal, oil mainly concentrates on the top layer of about 20cm to the pollution of soil, its adhesion strength is strong and emulsifying capacity is low, affect the permeability of soil, the content of Nitrogen In Soils, phosphorus can also be made to reduce simultaneously, these hazardous contaminants also likely by food chain enrichment step by step in animal and plant body, cause larger harm, and particularly polycyclic aromatic hydrocarbon wherein has teratogenesis, carcinogenic and mutability.
Because a large amount of PAH (polycyclic aromatic hydrocarbon) has teratogenesis, carcinogenic and mutagenicity, by direct and degradation pathways, grievous injury is brought to health, people are in polycyclic aromatic hydrocarbons contaminated environment for a long time will cause acute or chronic injury, be skin and respiratory tract to the topmost hazardous distinguish of human body according to investigations, likely cause cutaneum carcinoma, lung cancer and cancer of the stomach.In addition, after other harmful substance in oily sludge enters farmland, once be absorbed by crops, by food chain enrichment step by step in animal and plant body, finally will cause spreading of various bacterium, various disease occurs, threaten human health.Therefore, oil-polluted soils is administered imperative.
Nowadays, the process of Sludge in Oilfields is a very concerned difficult problem in Solid Waste Treatment.In fact, oily solid sludge is valuable secondary resource, regular collection is carried out to oily solid sludge, and developmental research goes out economical and practical method carries out harmless treatment and oil recovery to oily sludge and utilize, not only recyclable a large amount of energy, produce certain economic benefit, and can pollution abatement.
But; from the state of the art of comprehensive utilization; still lack the advanced treating of high added value at present and utilize technology; all should suit measures to local conditions in domestic various places; seek comprehensive utilization and solve greasy filth pollution problem rationally effective processing method, if a kind of economical and practical method can be developed carry out harmless treatment and Oil residue recuperation to oily sludge, not only can produce certain economic benefit; and meeting decreasing pollution, the final health for Environmental Protection Cause and the mankind makes huge contribution.
Summary of the invention
The object of the present invention is to provide a kind of petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad, process can be entered to petroleum-contaminated soil, effectively reduce the oil content in petroleum-contaminated soil.
The technical solution adopted in the present invention is, based on the petroleum-contaminated soil biodegrading process that Plesiomonas, pseudomonad are combined with electric field, specifically implements according to following steps:
Step 1, Plesiomonas and pseudomonas is utilized to prepare petroleum-contaminated soil microbial inoculum;
Step 2, take pending petroleum-contaminated soil, pre-treatment is carried out to pending petroleum-contaminated soil, the petroleum-contaminated soil after pre-treatment is filled into petroleum-contaminated soil and repairs in case;
Step 3, the petroleum-contaminated soil microbial inoculum prepared in step 1 is joined in step 2 in the petroleum-contaminated soil repaired in case, and apply electric field to the petroleum-contaminated soil repaired in case, under the acting in conjunction of petroleum-contaminated soil microbial inoculum and electric field, complete the degraded to petroleum-contaminated soil.
Feature of the present invention is also:
Step 1.1, be that 1 ~ 3:1 gets Plesiomonas, pseudomonas respectively according to volume ratio;
Step 1.2, the Plesiomonas in step 1.1, pseudomonas are put into two fermentation tank sealing and fermenting respectively, fermentation time is 45h ~ 48h;
Step 1.3, after step 1.2, Plesiomonas, pseudomonas after fermentation to be mixed, form mixed bacteria liquid;
Step 1.4, take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add in the mixed bacteria liquid that step 1.3 obtains together, after stirring, prepare process petroleum-contaminated soil microbial inoculum.
The ammonium nitrate of 0.032g and the potassium dihydrogen phosphate of 0.004g to be added in the mixed bacteria liquid of often liter in step 1.4.
Step 2 is specifically implemented according to following steps:
Step 2.1, be that 27 ~ 29:1 takes pending petroleum-contaminated soil, sawdust respectively in mass ratio;
Wherein, the oil content in pending petroleum-contaminated soil is 8% ~ 12%;
Step 2.2, the pending petroleum-contaminated soil taken in step 2.1 and sawdust to be mixed, adopt the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil;
Step 2.3, the petroleum-contaminated soil after step 2.2 pre-treatment loaded and repairs in case.
In step 2.3 charge weight of petroleum-contaminated soil be repair casing long-pending 80% ~ 85%.
Step 3 is concrete to be implemented in accordance with the following methods:
The petroleum-contaminated soil microbial inoculum prepared in step 1 is added in the petroleum-contaminated soil in the reparation case in step 2, wherein petroleum-contaminated soil microbial inoculum addition is 4.5% ~ 5.5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
For repairing the impulse electric field of petroleum-contaminated soil in case in step 3, its electric-field intensity is 100V/m, and frequency is 25Hz; Impulse electric field acts on 4h every day, applies electric field at twice, acts on 2h at every turn.
Beneficial effect of the present invention is:
(1) the present invention is based on Plesiomonas, petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field, having drawn every technology and the remedying oil-polluted soils feasibility in microorganism remediation oil-polluted soils field, is a repairing method of microorganism having supremacy clause.
(2) the reparation box structure the present invention is based on Plesiomonas, relating in petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field is simple, be exactly common cuboid or square waterproof bamboo slab rubber chest, be very easy to obtained, bring conveniently to whole biodegrading process.
(3) the present invention is based on Plesiomonas, petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field, what operate very is simple, local and among a small circle in be comparatively cost saving in all multi-schemes such as remedying oil-polluted soils, can effectively reduce costs.
(4) the present invention is based on Plesiomonas, petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field, except remedying oil-polluted soils, can also repair other contaminated soils, as: the reparation of crossing the aspect such as strong for the soil of pollution by pesticides, soil ph.
Accompanying drawing explanation
Fig. 1 to adopt after petroleum-contaminated soil biodegrading process process petroleum-contaminated soil of the present invention oil content time history plot in petroleum-contaminated soil.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
The present invention is based on Plesiomonas, petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field, specifically implement according to following steps:
Step 1, utilize Plesiomonas and pseudomonas to prepare petroleum-contaminated soil microbial inoculum, specifically implement according to following steps:
Step 1.1, be that 1 ~ 3:1 gets Plesiomonas, pseudomonas respectively according to volume ratio;
Step 1.2, the Plesiomonas in step 1.1, pseudomonas are put into two fermentation tank sealing and fermenting respectively, fermentation time is 45h ~ 48h;
Step 1.3, after step 1.2, Plesiomonas, pseudomonas after fermentation to be mixed, form mixed bacteria liquid;
Step 1.4, take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add in the mixed bacteria liquid that step 1.3 obtains together, after stirring, prepare process petroleum-contaminated soil microbial inoculum;
In step 1.4, the ammonium nitrate of 0.032g and the potassium dihydrogen phosphate of 0.004g in the mixed bacteria liquid of often liter, to be added.
Step 2, take pending petroleum-contaminated soil, pre-treatment carried out to pending petroleum-contaminated soil, the petroleum-contaminated soil after pre-treatment is filled into petroleum-contaminated soil and repairs in case, specifically implement according to following steps:
Step 2.1, be that 27 ~ 29:1 takes pending petroleum-contaminated soil, sawdust respectively in mass ratio;
Wherein, the oil content in pending petroleum-contaminated soil is 8% ~ 12%;
Step 2.2, the pending petroleum-contaminated soil taken in step 2.1 and sawdust to be mixed, adopt the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil;
Step 2.3, the petroleum-contaminated soil after step 2.2 pre-treatment loaded and repairs in case;
The charge weight of usual petroleum-contaminated soil be repair casing long-pending 80% ~ 85%;
Repairing case is cuboid or cube-shaped, and its top, by opening, is repaired case and adopted waterproof bamboo slab rubber to make.
Step 3, the petroleum-contaminated soil microbial inoculum prepared in step 1 is joined in step 2 in the petroleum-contaminated soil repaired in case, and apply electric field to the petroleum-contaminated soil repaired in case, under the acting in conjunction of petroleum-contaminated soil microbial inoculum and electric field, complete the degraded to petroleum-contaminated soil, specifically implement in accordance with the following methods:
The petroleum-contaminated soil microbial inoculum prepared in step 1 is added in the petroleum-contaminated soil in the reparation case in step 2, wherein petroleum-contaminated soil microbial inoculum addition is 4.5% ~ 5.5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case, and concrete mode is as follows:
Adopt impulse electric field, electric-field intensity is 100V/m, and frequency is 25Hz; Every day acts on 4h, applies electric field at twice, acts on 2h at every turn.
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
Utilize and the present invention is based on Plesiomonas, in process that petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field is repaired petroleum-contaminated soil, take with under type in order to verify to the degradation effect of petroleum-contaminated soil:
Petroleum-contaminated soil in case is repaired once every within 2 days, can to get, adopt sampler to sample when getting petroleum-contaminated soil, when getting petroleum-contaminated soil at every turn, the degree of depth is 15cm ~ 30cm, the petroleum-contaminated soil amount of each taking-up wants consistent, measure the oil content of the petroleum-contaminated soil taken out, will survey afterwards after measured petroleum-contaminated soil loads sample bottle and sample bottle is sealed, and on sample bottle, mark time on the same day, process numbering and oil content;
The condition measuring oil content in petroleum-contaminated soil measures under wavelength is 226nm condition at ultraviolet-ultrasonic wave:
As shown in Figure 1, after curve wherein represents the petroleum-contaminated soil biodegrading process process petroleum-contaminated soil that employing the present invention is based on Plesiomonas, pseudomonad is combined with electric field, oil content situation over time in petroleum-contaminated soil; Can find out according to Fig. 1: through the processing procedure of 60 days, oil content in petroleum-contaminated soil degraded amplitude is comparatively large, at the end of banking up, its oil content by 10.04% drop to 1.38%, degradation rate is up to 86.21%;
The present invention is based on Plesiomonas, why good petroleum-contaminated soil biodegrading process effect that pseudomonad is combined with electric field is, its main cause is: under the invigoration effect of electric field, microbial cell surface current potential changes, improve the metabolism of cell, make its increased activity, thus promote that microorganism is to the degradation efficiency of petroleum-contaminated soil Petroleum Hydrocarbon.
Embodiment 1
Be that 1:1 gets Plesiomonas, pseudomonas respectively according to volume ratio; The Plesiomonas taken, pseudomonas are put into respectively two fermentation tank sealing and fermenting, fermentation time is 45h; By the Plesiomonas after fermentation, pseudomonas mixing, form mixed bacteria liquid; Take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add together in mixed bacteria liquid, prepare process petroleum-contaminated soil microbial inoculum, wherein will add the ammonium nitrate of 0.032g, the potassium dihydrogen phosphate of 0.004g in the mixed bacteria liquid of often liter;
Pending petroleum-contaminated soil, sawdust is taken respectively in mass ratio for 27:1; Oil content in wherein pending petroleum-contaminated soil is 8%; The pending petroleum-contaminated soil taken is mixed with sawdust, adopts the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil; Petroleum-contaminated soil after pre-treatment is loaded and repairs in case, the charge weight of petroleum-contaminated soil be repair casing long-pending 80%;
Added to by petroleum-contaminated soil microbial inoculum in the petroleum-contaminated soil repaired in case, wherein petroleum-contaminated soil microbial inoculum addition is 4.5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Petroleum-contaminated soil microbial inoculum is added after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case: electric-field intensity is 100V/m, and frequency is 25Hz, and every day acts on 4h, apply electric field (morning, afternoon) at twice, act on 2h at every turn;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
Embodiment 2
Be that 1.5:1 gets Plesiomonas, pseudomonas respectively according to volume ratio; The Plesiomonas taken, pseudomonas are put into respectively two fermentation tank sealing and fermenting, fermentation time is 46h; By the Plesiomonas after fermentation, pseudomonas mixing, form mixed bacteria liquid; Take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add together in mixed bacteria liquid, prepare process petroleum-contaminated soil microbial inoculum, wherein will add the ammonium nitrate of 0.032g, the potassium dihydrogen phosphate of 0.004g in the mixed bacteria liquid of often liter;
Pending petroleum-contaminated soil, sawdust is taken respectively in mass ratio for 27.5:1; Oil content in wherein pending petroleum-contaminated soil is 9%; The pending petroleum-contaminated soil taken is mixed with sawdust, adopts the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil; Petroleum-contaminated soil after pre-treatment is loaded and repairs in case, the charge weight of petroleum-contaminated soil be repair casing long-pending 83%;
Added to by petroleum-contaminated soil microbial inoculum in the petroleum-contaminated soil repaired in case, wherein petroleum-contaminated soil microbial inoculum addition is 4.8% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case: electric-field intensity is 100V/m, and frequency is 25Hz, and every day acts on 4h, applies electric field at twice, acts on 2h at every turn;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
Embodiment 3
Be that 2:1 gets Plesiomonas, pseudomonas respectively according to volume ratio; The Plesiomonas taken, pseudomonas are put into respectively two fermentation tank sealing and fermenting, fermentation time is 47h; By the Plesiomonas after fermentation, pseudomonas mixing, form mixed bacteria liquid; Take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add together in mixed bacteria liquid, prepare process petroleum-contaminated soil microbial inoculum, wherein will add the ammonium nitrate of 0.032g, the potassium dihydrogen phosphate of 0.004g in the mixed bacteria liquid of often liter;
Pending petroleum-contaminated soil, sawdust is taken respectively in mass ratio for 28:1; Oil content in wherein pending petroleum-contaminated soil is 10%; The pending petroleum-contaminated soil taken is mixed with sawdust, adopts the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil; Petroleum-contaminated soil after pre-treatment is loaded and repairs in case, the charge weight of petroleum-contaminated soil be repair casing long-pending 84%;
Added to by petroleum-contaminated soil microbial inoculum in the petroleum-contaminated soil repaired in case, wherein petroleum-contaminated soil microbial inoculum addition is 5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case: electric-field intensity is 100V/m, and frequency is 25Hz, and every day acts on 4h, applies electric field at twice, acts on 2h at every turn;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
Embodiment 4
Be that 3:1 gets Plesiomonas, pseudomonas respectively according to volume ratio; The Plesiomonas taken, pseudomonas are put into respectively two fermentation tank sealing and fermenting, fermentation time is 47h; By the Plesiomonas after fermentation, pseudomonas mixing, form mixed bacteria liquid; Take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add together in mixed bacteria liquid, prepare process petroleum-contaminated soil microbial inoculum, wherein will add the ammonium nitrate of 0.032g, the potassium dihydrogen phosphate of 0.004g in the mixed bacteria liquid of often liter;
Pending petroleum-contaminated soil, sawdust is taken respectively in mass ratio for 28:1; Oil content in wherein pending petroleum-contaminated soil is 12%; The pending petroleum-contaminated soil taken is mixed with sawdust, adopts the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil; Petroleum-contaminated soil after pre-treatment is loaded and repairs in case, the charge weight of petroleum-contaminated soil be repair casing long-pending 85%;
Added to by petroleum-contaminated soil microbial inoculum in the petroleum-contaminated soil repaired in case, wherein petroleum-contaminated soil microbial inoculum addition is 5.5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case: electric-field intensity is 100V/m, and frequency is 25Hz, and every day acts on 4h, applies electric field at twice, acts on 2h at every turn;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
The present invention is based on Plesiomonas, petroleum-contaminated soil biodegrading process that pseudomonad is combined with electric field; can process petroleum-contaminated soil; effectively reduce the oil content in petroleum-contaminated soil; petroleum-contaminated soil after degradation treatment can be recovered utilization; like this; not only can produce certain economic benefit, and meeting decreasing pollution, the final health for Environmental Protection Cause and the mankind makes huge contribution.
Claims (7)
1. based on the petroleum-contaminated soil biodegrading process that Plesiomonas, pseudomonad are combined with electric field, it is characterized in that, specifically implement according to following steps:
Step 1, Plesiomonas and pseudomonas is utilized to prepare petroleum-contaminated soil microbial inoculum;
Step 2, take pending petroleum-contaminated soil, pre-treatment is carried out to pending petroleum-contaminated soil, the petroleum-contaminated soil after pre-treatment is filled into petroleum-contaminated soil and repairs in case;
Step 3, the petroleum-contaminated soil microbial inoculum prepared in step 1 is joined in step 2 in the petroleum-contaminated soil repaired in case, and apply electric field to the petroleum-contaminated soil repaired in case, under the acting in conjunction of petroleum-contaminated soil microbial inoculum and electric field, complete the degraded to petroleum-contaminated soil.
2. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 1, it is characterized in that, described step 1 is specifically implemented according to following steps:
Step 1.1, be that 1 ~ 3:1 gets Plesiomonas, pseudomonas respectively according to volume ratio;
Step 1.2, the Plesiomonas in step 1.1, pseudomonas are put into two fermentation tank sealing and fermenting respectively, fermentation time is 45h ~ 48h;
Step 1.3, after step 1.2, Plesiomonas, pseudomonas after fermentation to be mixed, form mixed bacteria liquid;
Step 1.4, take ammonium nitrate NH respectively
4nO
3, potassium dihydrogen phosphate KH
2pO
4, and the ammonium nitrate NH that will take
4nO
3, potassium dihydrogen phosphate KH
2pO
4add in the mixed bacteria liquid that step 1.3 obtains together, after stirring, prepare process petroleum-contaminated soil microbial inoculum.
3. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 2, is characterized in that, will add the ammonium nitrate of 0.032g and the potassium dihydrogen phosphate of 0.004g in described step 1.4 in the mixed bacteria liquid of often liter.
4. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 1, it is characterized in that, described step 2 is specifically implemented according to following steps:
Step 2.1, be that 27 ~ 29:1 takes pending petroleum-contaminated soil, sawdust respectively in mass ratio;
Wherein, the oil content in pending petroleum-contaminated soil is 8% ~ 12%;
Step 2.2, the pending petroleum-contaminated soil taken in step 2.1 and sawdust to be mixed, adopt the mode stirred that pending petroleum-contaminated soil is mixed with sawdust, complete the pre-treatment to pending petroleum-contaminated soil;
Step 2.3, the petroleum-contaminated soil after step 2.2 pre-treatment loaded and repairs in case.
5. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 4, is characterized in that, in described step 2.3 charge weight of petroleum-contaminated soil be repair casing long-pending 80% ~ 85%.
6. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 1, is characterized in that, described step 3 is concrete to be implemented in accordance with the following methods:
The petroleum-contaminated soil microbial inoculum prepared in step 1 is added in the petroleum-contaminated soil in the reparation case in step 2, wherein petroleum-contaminated soil microbial inoculum addition is 4.5% ~ 5.5% of the petroleum-contaminated soil quality of repairing in case, utilizes petroleum-contaminated soil microbial inoculum to carry out process 60 days to petroleum-contaminated soil; In these 60 days, to the petroleum-contaminated soil repaired in case be stirred every 5 days, make petroleum-contaminated soil microbial inoculum fully enter into petroleum-contaminated soil;
Added by petroleum-contaminated soil microbial inoculum after in the petroleum-contaminated soil repaired in case, every day all will by Electro-pulsing Field in repairing the petroleum-contaminated soil in case;
The moisture content repairing petroleum-contaminated soil in case will be measured every day, when moisture content lower than 45% time to carry out moisturizing, until moisture content reaches 45%;
After 60 days, namely complete the process to petroleum-contaminated soil.
7. petroleum-contaminated soil biodegrading process of combining with electric field based on Plesiomonas, pseudomonad according to claim 6, is characterized in that, for repairing the impulse electric field of petroleum-contaminated soil in case in described step 3, its electric-field intensity is 100V/m, and frequency is 25Hz;
Impulse electric field acts on 4h every day, applies electric field at twice, acts on 2h at every turn.
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| CN105945063A (en) * | 2016-07-19 | 2016-09-21 | 中国科学院沈阳应用生态研究所 | On-line water supplementary device and method for maintaining polluted soil electrical restoration efficiency |
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