CN103567220A - In-situ repairing method of microorganisms of petroleum polluted soil - Google Patents
In-situ repairing method of microorganisms of petroleum polluted soil Download PDFInfo
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Abstract
The invention relates to an in-situ repairing method of microorganisms of petroleum polluted soil. The method comprises the following steps: (1) culturing a flora; (2) activating the flora; (3) preparing a microorganism growth accelerating agent; (4) preparation a nutrient solution; (5) preparing a bio-surfactant; (6) dosing for the first time; (7) repairing. According to the in-situ repairing method of microorganisms of petroleum polluted soil, the petroleum microorganism degraded flora, the microorganism growth accelerating agent and the bio-surfactant are used as main components, certain microorganism nutrients are supplied, the optimal petroleum microorganism transformed flora can multiply at a high speed by adding the microorganism growth accelerating agent while being dosed into soil, so that the transformation and degrading efficiency of petroleum elements in the soil can be greatly improved, the problems of long duration and slow response in a petroleum polluted soil microorganism in-situ repairing method can be solved, and the microorganism in-situ repairing technology becomes a reliable, efficient and operable petroleum polluted soil control and management means.
Description
Technical field
The invention belongs to oil-polluted soils and administer field, relate to microorganism in situ reparation, especially a kind of microorganism in situ restorative procedure of oil-polluted soils.
Background technology
Soil pollution is apparently higher than original content of soil, to surpass the self-purification capacity of soil because mankind's activity causes the content of some harmful components, cause that thus composition of the soil, 26S Proteasome Structure and Function change, harmful substance or its catabolite be accumulation gradually in soil, reaches the degree being detrimental to health.Oil-polluted soils results from many links such as oil exploration, exploitation, transportation, storage, processing, accidental leakage, is the discarded object that petroleum industry produces.Oily sludge contains the poisonous and harmful substances such as macromolecule organic, if do not carry out, does not effectively properly dispose, and can cause the severe contamination of soil, underground water, water system and ocean, and health is worked the mischief and affected.
More than totally 400 of the oil gas field of China's exploration, exploitation, is distributed in 25 provinces，municipalities and autonomous regions in the whole nation, nearly 200,000 square kilometres of field area working range.According to incompletely statistics, there are more than 20 ten thousand places in internal contamination place, field area, and high concentration petroleum pollution soil and oil sediment burden exceed 2,000,000 tons.North China Oilfield a lot of farmlands around cannot be cultivated due to crude oil pollution, all will pay substantial contribution as the reparation to peasant every year; Yellow River System annual oil-containing reaches as high as 4.82mg/L, and Liaohe River water system annual oil-containing reaches as high as 7.68mg/L, has surpassed significantly the standard (< 0.1mg/L) of three grades of surface water of country; Some areas even pollute groundwater resources, as more than the In Zibo, Shandong the highest oil-containing of underground water reached 100mg/L, surpass national standard (< 0.1mg/L) more than 1000 times.
Restriction due to conditions such as cost, technology, the oil-polluted soils of each elephant or put together is deposited, but after depositing, conventionally do not do further processing or carry out on a small scale but the higher technical finesse of cost, for example: partition method, chemistry stir partition method, heat is washed method, pyrolysis processing method, spiral grate incinerator method, organic solvent extractionprocess, prefabricated bed method, bioreactor method etc. from revolving in ultrasonic radiation.These method great majority belong to showering method, need to move contaminated soil and focus on, not only with high costs, treating capacity is limited, and easily causes secondary pollution.
Microorganism in situ recovery technique refers to the biological control measure that utilizes specific microorganism absorption, conversion, removing or degraded soil petroleum pollution thing to realize soil sanitation.This technology can make petroleum pollution remove from soil, less to surrounding environment influence, can not produce secondary pollution, the huge soil remediation potentiality that have.But the microorganism recovery technique of existing oil-polluted soils has some limitations, as microorganism is repaired, the duration is long, effect is thorough, be not suitable for the reparation of severe contamination soil etc.
Large quantity research shows, a principal element of restriction oil-polluted soils microorganism in situ recovery technique development is that contaminated soil lacks enough oxygen and nutrition conventionally for the microorganism Fast Growth of degraded oil, so that the improvement potentiality that these microorganisms possess self are not in full use, and fail to become comparatively desirable soil petroleum pollution control measures.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, a kind of microorganism in situ restorative procedure Soil Microorganism Fast Growth and the oil-polluted soils that degradation rate is very high that promotes is provided, utilize hydrocarbon degradation bacterium to carry out the repair process of oil-polluted soils, hydrocarbons in soil (sump oil) is resolved into carbon dioxide and water, reach the object of eliminating oil pollution.
The present invention is achieved through the following technical solutions goal of the invention:
A microorganism in situ restorative procedure, the steps include:
(1) bacteria group culture: select to have the microbial degradation flora body of oil degradation characteristic, flora adopts the mode of solid culture, and the wheat bran of take is cultivated domestication as carrier, gets the powdered substance that its intermediate product mixing also forms after mummification;
(2) flora activates: the powdered substance by step in is (1) put into water, soaks 0.5～2h, removes wheat bran and obtains the flora liquid after activation;
(3) growth of microorganism accelerator preparation: compound amino acid, composite trace element, activating enzymes and humic acid are put into water, stir, obtain growth of microorganism accelerator;
(4) compounding: add microbial nutrition element in growth of microorganism accelerator, add water and diluted, obtain growth of microorganism accelerator nutrient solution;
(5) biosurfactant preparation: comprise water, sodium alkyl benzene sulfonate and microorganism fermentation extractive matter protein, sodium alkyl benzene sulfonate, microorganism fermentation extractive matter protein are put into water, stir, obtain biosurfactant;
(6) just throw: by disposable the pouring into by the soil of oil pollution of flora liquid after activating, the growth of microorganism accelerator nutrient solution of getting gross weight 20% is sprayed in the soil of oil pollution, plowed soils, fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(7) repair: after just throwing, growth of microorganism accelerator nutrient solution and biosurfactant are mixed, obtain mixed liquor, this mixed liquor is divided into 3～6 equal portions, every 10 days to evenly spraying a mixed liquor in contaminated soil, while spraying, augments moisture and the contaminated soil of the turning over oxygen that exposes to the sun at every turn; When the oil content of soil drop to percentage by weight below 1% after, spray frequency and be adjusted into every 15 days and spray mixed once liquid, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, until meet the requirements of index.
And, step (1) described microbial degradation flora body is avirulence bacterial community and/or heterotroph micropopulation, this microbial degradation flora body is the compound population of bacterium, Fungal community composition, and bacterium is leather Albert'stain Albert negative bacillus, and fungi is Mucor, Cunninghamella sp Pseudomonas.
And step (2) described powdered substance and water weight ratio is 1～2:10.
And the step (3) weight ratio of described compound amino acid, composite trace element, activating enzymes, humic acid and water is: 1:1:1:2:4～5.
And step (4) described microbial nutrition element, growth of microorganism accelerator and the water weight ratio that feeds intake is: 1:2～3:20～25.
And step (4) described microbial nutrition element comprises protein, sugar and inorganic salts, its weight ratio is protein: sugar: inorganic salts=3:1～3:2.
And component and parts by weight that described inorganic salts are included are respectively:
0.1 part～0.2 part, sodium chloride, 0.1 part～0.2 part of ammonia, 0.2 part～0.4 part, ammonium sulfate, 0.2 part～0.4 part of potassium dihydrogen phosphate, 0.2 part～0.4 part of dipotassium hydrogen phosphate, 0.06 part～0.12 part, magnesium sulfate, 0.05 part～0.1 part, zinc sulfate, 0.03 part～0.06 part, calcium chloride, 0.02 part～0.04 part, calcium nitrate, 0.02 part～0.04 part, ferric sulfate, 0.02 part～0.04 part of manganese sulfate.
And step (5) described biosurfactant component and percentage by weight thereof is:
Sodium alkyl benzene sulfonate 9%～13.5%
Microorganism fermentation extractive matter protein 1%～1.5%
Surplus is water;
And step is (6) described is 30cm by the thickness of soil of oil pollution, area is 100m
2.
And, described growth of microorganism accelerator nutrient solution: biosurfactant mixed weight is than being 3:1.
The present invention has following advantage and good effect:
1, in the microorganism in situ restorative procedure of oil-polluted soils provided by the invention by petroleum microorganism degradation flora, growth of microorganism accelerator is done, biosurfactant is main component, supplemented again certain microbial nutrition element, dropping into preferred petroleum microorganism conversion flora in soil when, increasing growth of microorganism accelerator impels it to breed at a high speed, thereby conversion and the degradation efficiency of soil PetroChina Company Limited. element have greatly been improved, the time having solved in oil-polluted soils microorganism in situ restorative procedure is long, slow problem takes effect, microorganism in situ recovery technique is become reliably, efficiently, exercisable oil-polluted soils governing measure.
2, the present invention can be applicable to the oil-containing solids discarded object that produces in the leakage of fuel oil, crude oil, grease and process of oil production or the processing of oily sludge, according to the different of oil content in contaminated soil with to processing the difference that in rear soil, residual oil content requires, processing time is generally 16～26 weeks, during processing, environment temperature requires 10 ℃～30 ℃, and environment is had to good applicability.
3, the biosurfactant that the present invention adopts is mainly used in improving the dispersing characteristic of crude oil, promotes the bacterium that exists with aqueous solution state and the contact area of crude oil, and then reaches the effect of enhanced biodegradation.
The specific embodiment
Below by specific embodiment, further narrate the present invention, it should be noted that following examples are illustrative, but not determinate.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Embodiment 1
A microorganism in situ restorative procedure, the steps include:
(1) bacteria group culture: selection can transform avirulence bacterial community and the heterotroph micropopulation microbial degradation flora body of petroleum characteristics, adopt the mode of solid culture, the wheat bran of take is cultivated domestication as carrier, gets its middle bacterial product and mixes the powdered substance forming after also mummification;
(2) flora activates: get the powdered substance 10Kg of step in (1) and put into 100Kg water, soak 0.5～2h, remove wheat bran and obtain the flora liquid after activation;
(3) growth of microorganism accelerator preparation: 4Kg compound amino acid, 4Kg composite trace element, 4Kg activating enzymes and 8Kg humic acid are put into 16Kg water, stir, obtain growth of microorganism agent;
(4) compounding: add 12Kg microbial nutrition element in growth of microorganism accelerator, microbial nutrition element comprises organic matter and inorganic salts, wherein organic matter comprises 6Kg protein and 2Kg sugar, inorganic salts are 4Kg, its component is sodium chloride, ammonia, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, zinc sulfate, calcium chloride, calcium nitrate, ferric sulfate, manganese sulfate, each composition weight umber is: sodium chloride 0.4Kg, ammonia 0.4Kg, ammonium sulfate 0.6Kg, potassium dihydrogen phosphate 0.6Kg, dipotassium hydrogen phosphate 0.6Kg, magnesium sulfate 0.24Kg, zinc sulfate 0.2Kg, calcium chloride 0.12Kg, calcium nitrate 0.08Kg, ferric sulfate 0.08Kg, manganese sulfate 0.08Kg, adding 240Kg water is diluted, obtain growth of microorganism agent nutrient solution,
(5) biosurfactant preparation: biosurfactant weight is 96Kg, and the contained component of biosurfactant and weight thereof are:
Sodium alkyl benzene sulfonate 8.64Kg;
Microorganism fermentation extractive matter protein 1.44Kg;
Surplus is water;
(6) just throw: by the flora liquid after activating, disposable to pour into by the thickness of oil pollution be 30cm, and area is 100m
2in soil, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of oil pollution, and plowed soils fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(7) repair: after just throwing, growth of microorganism agent nutrient solution and biosurfactant are mixed, obtain mixed liquor, this mixed liquor is divided into 3～6 equal portions, every 10 days to evenly spraying a mixed liquor in contaminated soil, while spraying, augments moisture and the contaminated soil of the turning over oxygen that exposes to the sun at every turn; When the oil content of soil drop to percentage by weight below 1% after, spray frequency and be adjusted into every 15 days and spray mixed once liquid, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, until meet the requirements of index.According to the different of oil content in contaminated soil with to processing the difference that in rear soil, residual oil content requires, the processing time is generally 16～26 weeks, and during processing, environment temperature requires 10 ℃～30 ℃.
Embodiment 2
A microorganism in situ restorative procedure, the steps include:
(1) bacteria group culture: selection can transform avirulence bacterial community and the heterotroph micropopulation microbial degradation flora body of petroleum characteristics, adopt the mode of solid culture, the wheat bran of take is cultivated domestication as carrier, gets its middle bacterial product and mixes the powdered substance forming after also mummification;
(2) flora activates: get the powdered substance 20Kg of step in (1) and put into 100Kg water, soak 0.5～2h, remove wheat bran and obtain the flora liquid after activation;
(3) growth of microorganism accelerator preparation: 8Kg compound amino acid, 8Kg composite trace element, 8Kg activating enzymes and 16Kg humic acid are put into 40Kg water, stir, obtain growth of microorganism agent;
(4) compounding: add 40Kg microbial nutrition element in growth of microorganism accelerator, microbial nutrition element comprises organic matter and inorganic salts, wherein organic matter comprises 15Kg protein and 15Kg sugar, inorganic salts are 10Kg, its component is sodium chloride, ammonia, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, zinc sulfate, calcium chloride, calcium nitrate, ferric sulfate, manganese sulfate, each composition weight umber is: sodium chloride 1Kg, ammonia 1Kg, ammonium sulfate 2Kg, potassium dihydrogen phosphate 2Kg, dipotassium hydrogen phosphate 2Kg, magnesium sulfate 0.6Kg, zinc sulfate 0.5Kg, calcium chloride 0.3Kg, calcium nitrate 0.2Kg, ferric sulfate 0.2Kg, manganese sulfate 0.2Kg, adding 900Kg water is diluted, obtain growth of microorganism agent nutrient solution,
(5) biosurfactant preparation: biosurfactant weight is 340Kg, and the contained component of biosurfactant and weight thereof are:
Sodium alkyl benzene sulfonate 45.9Kg;
Microorganism fermentation extractive matter protein 3.4Kg;
Surplus is water;
(6) just throw: by the flora liquid after activating, disposable to pour into by the thickness of oil pollution be 30cm, and area is 100m
2in soil, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of oil pollution, and plowed soils fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(7) repair: after just throwing, growth of microorganism agent nutrient solution and biosurfactant are mixed, obtain mixed liquor, this mixed liquor is divided into 3～6 equal portions, every 10 days to evenly spraying a mixed liquor in contaminated soil, while spraying, augments moisture and the contaminated soil of the turning over oxygen that exposes to the sun at every turn; When the oil content of soil drop to percentage by weight below 1% after, spray frequency and be adjusted into every 15 days and spray mixed once liquid, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, until meet the requirements of index.According to the different of oil content in contaminated soil with to processing the difference that in rear soil, residual oil content requires, the processing time is generally 16～26 weeks, and during processing, environment temperature requires 10 ℃～30 ℃.
Embodiment 3
A microorganism in situ restorative procedure, the steps include:
(1) bacteria group culture: selection can transform avirulence bacterial community and the heterotroph micropopulation microbial degradation flora body of petroleum characteristics, adopt the mode of solid culture, the wheat bran of take is cultivated domestication as carrier, gets its middle bacterial product and mixes the powdered substance forming after also mummification;
(2) flora activates: get the powdered substance 20Kg of step in (1) and put into 100Kg water, soak 0.5～2h, remove wheat bran and obtain the flora liquid after activation;
(3) growth of microorganism accelerator preparation: 8Kg compound amino acid, 8Kg composite trace element, 8Kg activating enzymes and 16Kg humic acid are put into 40Kg water, stir, obtain growth of microorganism agent;
(4) compounding: add 40Kg microbial nutrition element in growth of microorganism accelerator, microbial nutrition element comprises organic matter and inorganic salts, wherein organic matter comprises 15Kg protein and 15Kg sugar, inorganic salts are 10Kg, its component is sodium chloride, ammonia, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, zinc sulfate, calcium chloride, calcium nitrate, ferric sulfate, manganese sulfate, each composition weight umber is: sodium chloride 1Kg, ammonia 1Kg, ammonium sulfate 2Kg, potassium dihydrogen phosphate 2Kg, dipotassium hydrogen phosphate 2Kg, magnesium sulfate 0.6Kg, zinc sulfate 0.5Kg, calcium chloride 0.3Kg, calcium nitrate 0.2Kg, ferric sulfate 0.2Kg, manganese sulfate 0.2Kg, adding 900Kg water is diluted, obtain growth of microorganism agent nutrient solution,
(5) biosurfactant preparation: biosurfactant weight is 340Kg, and the contained component of biosurfactant and weight thereof are:
Sodium alkyl benzene sulfonate 34Kg;
Microorganism fermentation extractive matter protein 4.08Kg;
Surplus is water;
(6) just throw: by the flora liquid after activating, disposable to pour into by the thickness of oil pollution be 30cm, and area is 100m
2in soil, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of oil pollution, and plowed soils fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(7) repair: after just throwing, growth of microorganism agent nutrient solution and biosurfactant are mixed, obtain mixed liquor, this mixed liquor is divided into 3～6 equal portions, every 10 days to evenly spraying a mixed liquor in contaminated soil, while spraying, augments moisture and the contaminated soil of the turning over oxygen that exposes to the sun at every turn; When the oil content of soil drop to percentage by weight below 1% after, spray frequency and be adjusted into every 15 days and spray mixed once liquid, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, until meet the requirements of index.According to the different of oil content in contaminated soil with to processing the difference that in rear soil, residual oil content requires, the processing time is generally 16～26 weeks, and during processing, environment temperature requires 10 ℃～30 ℃.
Simulated experiment:
In laboratory, utilize the sump oil contaminated soil of simulation preparation to carry out test, soil sample is taken from untainted the earth soil sample, and crude oil is taken from the aging crude oil after dehydration, substantially not moisture after tested.Its process for preparation is: first, from obtaining the earth air-dry, put into the plastic big basin that diameter is about 90cm, the degree of depth is about 25cm, claims the weight of its soil.Then according to the percentage of soil weight, add the crude oil (45 ℃ of left and right of crude oil temperature) after dehydration, be uniformly mixed the sump oil contaminated soil that forms simulation.The indoor factorial experiments analysis of having carried out respectively the evaluation test for the treatment of effect and having affected treatment effect.The oil content of test requirements document soil is below 15%.The simulating pollution soil that is 5% and 15% to oil content has respectively carried out test evaluation research.
(1) when soil oil content is 5%, degrade after one month, the oil content in soil can reach (1.9%) below 2%; After two months, the oil content in soil can drop to below 0.7%; After three months, the oil content in soil can drop to (0.18%) below 0.3%, has reached pollutant control criterion GB4284-84 mineral oil in fluid content's index in national agricultural mud.
(2) when soil oil content is 15%, degrade after one month, the oil content in soil is below 7%; After two months, the oil content in soil is that after 5.5%, four month, the oil content in soil reaches below 2%.
Site test:
According to the analysis result of the present situation of Daqing oil field crude oil pollution and improvement, respectively three kinds of dissimilar solid-state oily wastes have been carried out to three kinds of dissimilar biological prosthetic experimental studies.The biological prosthetic test of the first type is to take the biological prosthetic test of the oil-containing soil that well site that oil well operation pollutes is main body; The biological prosthetic test of the second type is to take the biological prosthetic test that the oily sludge removed in various container handlings and equipment in multi-purpose station is main body; The biological prosthetic test of the third type is to take the biological prosthetic test that the oily sludge of sedimentation is main body in the earth storage of ground crude oil recycling point.
By the field trial under the climatic environment condition of Daqing Area to three kinds of dissimilar solid oily wastes of oil field existence at present, take and process the treatment effect that measurement index that the oil content in rear solid waste is treatment effect is evaluated these two kinds of microorganism formulations, can draw the following conclusions:
(1) adopting this patent product is feasible to the biological prosthetic of oil contaminated soil, and the oil content of the oily sludge after processing can be controlled in national agricultural soil safety standard (0.3%).But when operation, consider the recycling of adaptability and the resource of microbe inoculation, initial oil content should be controlled at 15%(W/W) in.
(2), in whole process of the test, how making microniological proudcts and crude oil in contaminated soil fully contact is the key that assurance obtains good test effect, therefore, suggestion promote in the application machine method of mixing, guarantee the microorganism of inoculation and contacting of crude oil.
(3) by having experienced the biodegradation test effect analysis of 2 years, as the sump oil hole of oil extraction No.1 Factory, the recover the oil tracking and testing analytical test of each junior unit in the apricot 6-J2-104 well of four factories and apricot 2-40-620 well, while suspending test in by the end of October, 2010 in, oil content is respectively 27.1%, 3.8% and 7.97%, but when test in August, 2011, find that oil content is respectively 5.23%, 1.07% and 1.21%, by the follow-up sudden and violent oxygen of turning over, to in September, 2011, oil content is respectively 0.97%, 0.07% and 0.09%.Illustrate: the microorganism in Daqing Area severe cold winter still can survive, and again plays a role when temperature is suitable.
Claims (10)
1. a microorganism in situ restorative procedure for oil-polluted soils, is characterized in that: the steps include:
(1) bacteria group culture: select to have the microbial degradation flora body of oil degradation characteristic, flora adopts the mode of solid culture, and the wheat bran of take is cultivated domestication as carrier, gets the powdered substance that its intermediate product mixing also forms after mummification;
(2) flora activates: the powdered substance by step in is (1) put into water, soaks 0.5～2h, removes wheat bran and obtains the flora liquid after activation;
(3) growth of microorganism accelerator preparation: compound amino acid, composite trace element, activating enzymes and humic acid are put into water, stir, obtain growth of microorganism accelerator;
(4) compounding: add microbial nutrition element in growth of microorganism accelerator, add water and diluted, obtain growth of microorganism accelerator nutrient solution;
(5) biosurfactant preparation: comprise water, sodium alkyl benzene sulfonate and microorganism fermentation extractive matter protein, sodium alkyl benzene sulfonate, microorganism fermentation extractive matter protein are put into water, stir, obtain biosurfactant;
(6) just throw: by disposable the pouring into by the soil of oil pollution of flora liquid after activating, the growth of microorganism accelerator nutrient solution of getting gross weight 20% is sprayed in the soil of oil pollution, plowed soils, fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(7) repair: after just throwing, growth of microorganism accelerator nutrient solution and biosurfactant are mixed, obtain mixed liquor, this mixed liquor is divided into 3～6 equal portions, every 10 days to evenly spraying a mixed liquor in contaminated soil, while spraying, augments moisture and the contaminated soil of the turning over oxygen that exposes to the sun at every turn; When the oil content of soil drop to percentage by weight below 1% after, spray frequency and be adjusted into every 15 days and spray mixed once liquid, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, until meet the requirements of index.
2. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, it is characterized in that: step (1) described microbial degradation flora body is avirulence bacterial community and/or heterotroph micropopulation, this microbial degradation flora body is the compound population of bacterium, Fungal community composition, bacterium is leather Albert'stain Albert negative bacillus, and fungi is Mucor, Cunninghamella sp Pseudomonas.
3. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step (2) described powdered substance and water weight ratio is 1～2:10.
4. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: the step (3) weight ratio of described compound amino acid, composite trace element, activating enzymes, humic acid and water is: 1:1:1:2:4～5.
5. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step (4) described microbial nutrition element, growth of microorganism accelerator and the water weight ratio that feeds intake is: 1:2～3:20～25.
6. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step (4) described microbial nutrition element comprises protein, sugar and inorganic salts, and its weight ratio is protein: sugar: inorganic salts=3:1～3:2.
7. the microorganism in situ restorative procedure of oil-polluted soils according to claim 6, is characterized in that: component and parts by weight that described inorganic salts are included are respectively:
0.1 part～0.2 part, sodium chloride, 0.1 part～0.2 part of ammonia, 0.2 part～0.4 part, ammonium sulfate, 0.2 part～0.4 part of potassium dihydrogen phosphate, 0.2 part～0.4 part of dipotassium hydrogen phosphate, 0.06 part～0.12 part, magnesium sulfate, 0.05 part～0.1 part, zinc sulfate, 0.03 part～0.06 part, calcium chloride, 0.02 part～0.04 part, calcium nitrate, 0.02 part～0.04 part, ferric sulfate, 0.02 part～0.04 part of manganese sulfate.
8. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step (5) described biosurfactant component and percentage by weight thereof is:
Sodium alkyl benzene sulfonate 9%～13.5%
Microorganism fermentation extractive matter protein 1%～1.5%
Surplus is water.
9. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step is (6) described is 30cm by the thickness of soil of oil pollution, and area is 100m
2.
10. the microorganism in situ restorative procedure of oil-polluted soils according to claim 1, is characterized in that: step is described growth of microorganism accelerator nutrient solution (7): the weight ratio that biosurfactant mixes is 3:1.
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| CN111470640A (en) * | 2020-04-27 | 2020-07-31 | 安徽霖德生物科技有限公司 | Multi-dimensional in-situ bioremediation method for oil-containing bottom mud and water body |
| CN111620531A (en) * | 2020-04-24 | 2020-09-04 | 中国石油天然气股份有限公司 | Spraying agent for degrading oil sludge |
| CN111906140A (en) * | 2020-07-08 | 2020-11-10 | 西安建筑科技大学 | Method for degrading large amount of crude oil in soil through oleophilic organisms and activator thereof |
| CN112642857A (en) * | 2020-11-18 | 2021-04-13 | 山东高科联合环保科学研究院有限公司 | Marine microorganism remediation method for petroleum-polluted soil |
| CN113480375A (en) * | 2021-08-24 | 2021-10-08 | 中国石油天然气股份有限公司长庆油田分公司第五采油厂 | Oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382758A (en) * | 2001-04-20 | 2002-12-04 | 中国科学院沈阳应用生态研究所 | Process for preparing solid fungus to degradate petroleum |
| CN1413963A (en) * | 2002-09-19 | 2003-04-30 | 上海九康农业科技发展有限公司 | Compound application technology for nano-biological fertilizer and soil conditioner |
| CN101301657A (en) * | 2008-06-13 | 2008-11-12 | 天津市瑞丰原生物修复技术有限公司 | Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method |
| CN102453678A (en) * | 2010-10-26 | 2012-05-16 | 中国科学院生态环境研究中心 | Microorganism composite bacterial agent for restoration of saline alkali soil polluted by petroleum |
| CN103215204A (en) * | 2013-03-26 | 2013-07-24 | 中国石油天然气股份有限公司 | Arthrobacter strain for efficiently degrading phenanthrene and application thereof |
-
2013
- 2013-10-16 CN CN201310484371.7A patent/CN103567220A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382758A (en) * | 2001-04-20 | 2002-12-04 | 中国科学院沈阳应用生态研究所 | Process for preparing solid fungus to degradate petroleum |
| CN1413963A (en) * | 2002-09-19 | 2003-04-30 | 上海九康农业科技发展有限公司 | Compound application technology for nano-biological fertilizer and soil conditioner |
| CN101301657A (en) * | 2008-06-13 | 2008-11-12 | 天津市瑞丰原生物修复技术有限公司 | Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method |
| CN102453678A (en) * | 2010-10-26 | 2012-05-16 | 中国科学院生态环境研究中心 | Microorganism composite bacterial agent for restoration of saline alkali soil polluted by petroleum |
| CN103215204A (en) * | 2013-03-26 | 2013-07-24 | 中国石油天然气股份有限公司 | Arthrobacter strain for efficiently degrading phenanthrene and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王帅: "固定化法降解石油方法研究", 《贵州化工》, vol. 34, no. 3, 30 June 2009 (2009-06-30), pages 26 - 29 * |
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| CN108220196A (en) * | 2018-01-10 | 2018-06-29 | 南京林业大学 | A kind of method that microbial composite bacteria group handles oil pollution clay ground |
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| CN111470640A (en) * | 2020-04-27 | 2020-07-31 | 安徽霖德生物科技有限公司 | Multi-dimensional in-situ bioremediation method for oil-containing bottom mud and water body |
| CN111906140A (en) * | 2020-07-08 | 2020-11-10 | 西安建筑科技大学 | Method for degrading large amount of crude oil in soil through oleophilic organisms and activator thereof |
| CN112642857A (en) * | 2020-11-18 | 2021-04-13 | 山东高科联合环保科学研究院有限公司 | Marine microorganism remediation method for petroleum-polluted soil |
| CN113480375A (en) * | 2021-08-24 | 2021-10-08 | 中国石油天然气股份有限公司长庆油田分公司第五采油厂 | Oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method |
| CN115446108A (en) * | 2022-10-12 | 2022-12-09 | 西安交通大学 | Method for preparing biochar immobilized fungus-bacterium combined microbial inoculum for degrading oily sludge petroleum hydrocarbon from oily sludge |
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