CN108165509A - Compound micro-ecological preparation for the black smelly water harnessing in river and preparation method thereof - Google Patents
Compound micro-ecological preparation for the black smelly water harnessing in river and preparation method thereof Download PDFInfo
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- CN108165509A CN108165509A CN201711488397.3A CN201711488397A CN108165509A CN 108165509 A CN108165509 A CN 108165509A CN 201711488397 A CN201711488397 A CN 201711488397A CN 108165509 A CN108165509 A CN 108165509A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
Abstract
The invention discloses a kind of for compound micro-ecological preparation of the black smelly water harnessing in river and preparation method thereof, the compound micro-ecological preparation is made of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis, lactobacillus acidophilus, candida utili and actinomyces, is counted in mass ratio：Photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：Actinomyces are 2:2:1:1:1:1:2.The microbe species that the compound micro-ecological preparation includes are various, it is multiple functional, the major pollutants such as COD, ammonia nitrogen and the sulfide that can effectively degrade in black smelly water, improve water quality, repair river channel ecology system, and the black smelly water in most of rivers can be suitable for, the improvement of smelly water black to different rivers has certain versatility.
Description
Technical field
A kind of this bright microbiological treatment field for belonging to sewage, and in particular to composite microbial for the black smelly water harnessing in river
Object preparation and preparation method thereof.
Background technology
In recent years, along with the fast development of China's economy, the discharge of a large amount of city domestic sewages and industrial wastes, excess
The accumulation of organic matter alreadys exceed the bearing capacity in part river, leads to river water eutrophication, and dissolved oxygen is unbalance, and anaerobe is big
Amount breeding, the atraments such as foul gas and ferrous sulfide, manganese sulfide, the water bodys such as generation ammonia, hydrogen sulfide start to occur black smelly existing
As.The black smelly water in river not only influences city image, also the quality of water front construction and the health of resident can be caused greatly to endanger
Evil.State Council's publication in 2015《Water prevention and cure of pollution action plan》, it is distinctly claimed the year two thousand twenty ground level and more than completed region of the city
Black and odorous water is controlled within 10%, and the smelly water of urban black is eliminated to the year two thousand thirty.Therefore, the improvement quarter of the black smelly water in river is not allowed
It is slow.
The administering method of the black smelly water in river is mainly by Physical, chemical method and bioanalysis at present.Physical mainly includes clear
The means such as silt, running water drainage and watercourse aeration, but more engineering, equipment and energy consumption are needed, cost is higher, and the later stage still needs to
Bioanalysis is effected a radical cure.Chemical method is quickly repaired using adding chemical reagent, common agents have hydrogen peroxide, calper calcium peroxide and
Calcium nitrate, but it is often palliative in practice principle, and it be easy to cause secondary pollution.Bioanalysis refers to utilize biology
Organic pollution in the metabolic activity degradation water body of (including microorganism, plant and animal), fundamentally solves black smelly water and asks
Topic restores the ecosystem in river.Probiotics belong to bioanalysis, refer to through the technology of spreading cultivation, and microorganism is made liquid
Or the product of solid powder, polluted-water is added to by the way of certain, by the metabolic activity degradation water of microorganism
Pollutant, improve water quality.Probiotics will not be caused because making simple, easy to use, of low cost, significant effect
Secondary pollution and receive favor, be increasingly becoming the emphasis of research.
But the black smelly water constituent in river is complicated, not only COD contents are high, and ammonia nitrogen, total nitrogen, total phosphorus and metal ion are also all tight
Weight is exceeded, and the black smelly water in different rivers, and pollutant kind is also different, depends merely on certain single bacterial strain or certain a kind of bacterial strain
Manufactured probiotics are difficult with versatility to the different black smelly water in river.
Invention content
Based on the problems of prior art, the object of the present invention is to provide a kind of answering for the black smelly water harnessing in river
Probiotics and preparation method thereof, the COD in the black smelly water in river that can effectively degrade, ammonia nitrogen and sulfide are closed, is reduced smelly
Taste improves water quality, restores the ecological balance in river, and have certain versatility to the different black smelly water in river.
The purpose of the present invention is what is be achieved through the following technical solutions：
Embodiment of the present invention provides a kind of compound micro-ecological preparation for the black smelly water harnessing in river, the compound microecological
Preparation is by photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis, lactobacillus acidophilus, candida utili and unwrapping wire
Bacterium forms, and counts in mass ratio：Photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：It is false to produce protein
Silk yeast：Actinomyces are 2:2:1:1:1:1:2.
Embodiment of the present invention also provides a kind of preparation method of the compound micro-ecological preparation for the black smelly water harnessing in river,
Compound micro-ecological preparation of the present invention is used to prepare, is included the following steps：
By photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：
The mass ratio of actinomyces is 2:2:1:1:1:1:2 take respectively photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis,
Lactobacillus acidophilus, candida utili and actinomyces, by the photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis
Bacterium, lactobacillus acidophilus, candida utili and actinomyces are compounded, and after mixing, obtain the compound micro-ecological preparation.
As seen from the above technical solution provided by the invention, it is provided in an embodiment of the present invention to be controlled for the black smelly water in river
Compound micro-ecological preparation of reason and preparation method thereof, advantage are：
It is high for the black smelly water COD contents in river, ammonia nitrogen, total phosphorus, sulfide and the exceeded general character of metal ion, by will be more
The different microorganism of kind carries out compounding and forms a kind of compound micro-ecological preparation in specific proportions, wherein, photosynthetic bacteria can be in anaerobism
Under illumination or aerobic dark condition photosynthetic work is carried out as hydrogen donor and carbon source using organic matter, sulfide, ammonia in sewage etc.
With COD, sulfide, nitrogen and phosphorus in black smelly water of effectively degrading；Nitrobacteria belongs to aerobic bacteria, and denitrifying bacteria belongs to simultaneous
Property anaerobic bacteria shares out the work and help one another between them, by nitrification and denitrification, can effectively remove the ammonia in the smelly water of except black
Nitrogen and total nitrogen；Bacillus subtilis belongs to aerobic bacteria, can generate abundant amylase, protease, lipase and cellulase
Etc. a variety of enzymes, the complicated organic pollution such as polysaccharide, albumen and water soluble organic nitrogen is decomposed, effectively degrade COD, ammonia nitrogen and sulphur
Compound；Lactobacillus acidophilus belongs to facultative anaerobic bacteria, can carry out metabolic activity, degradable organic pollutant in the deep layer of black and odorous water；
Candida utili belongs to the eukaryotic microorganisms of amphimicrobian type, has very strong fit to high sugar, high-carbon, hyperosmosis environment
Ying Xing, the nitrogen substances such as organic matter, sulfide and nitrate, urea in the black smelly water that can effectively degrade；Actinomyces in
Aerobic bacteria not only has very strong enzyme system secretion capacity, and with very strong desulfurization and dephosphorizing capacity, can effectively degrade black
Sulfide and total phosphorus in smelly water.Mentioned microorganism includes aerobic microbiological and amphimicrobe, and it is black can be directed to river
The difference of smelly water different water levels dissolved oxygen, plays respective function, shares out the work and helps one another, COD, ammonia nitrogen in black smelly water of effectively degrading
River channel ecology system is repaired with major pollutants, improvement water quality such as sulfide.Microbe species in the present invention are various, work(
Can be complete, the black smelly water in most of rivers can be suitable for, the improvement of smelly water black to different rivers has certain versatility.
Description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, required use in being described below to embodiment
Attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only some embodiments of the present invention, for this
For the those of ordinary skill in field, without creative efforts, other are can also be obtained according to these attached drawings
Attached drawing.
Fig. 1 is compound micro-ecological preparation provided in an embodiment of the present invention smelly water major pollutant black to Tianjin river
Removal effect schematic diagram；
Fig. 2 is mainly dirty to the black smelly water in Hubei Province Xianning river for compound micro-ecological preparation provided in an embodiment of the present invention
Contaminate the removal effect schematic diagram of substance；
Fig. 3 is mainly dirty for compound micro-ecological preparation provided in an embodiment of the present invention smelly water black to Xinyang City, Henan Province river
Contaminate the removal effect schematic diagram of substance.
Specific embodiment
With reference to the particular content of the present invention, the technical solution in the embodiment of the present invention is clearly and completely retouched
It states, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the present invention
Embodiment, those of ordinary skill in the art's all other embodiments obtained without making creative work,
Belong to protection scope of the present invention.The content not being described in detail in the embodiment of the present invention belongs to professional and technical personnel in the field
The well known prior art.
The embodiment of the present invention provides a kind of compound micro-ecological preparation for the black smelly water harnessing in river, can effectively degrade
COD in the black smelly water in river, ammonia nitrogen and sulfide reduce stink, improve water quality, restore the ecological balance in river, and to not
The same black smelly water in river has certain versatility, and the compound micro-ecological preparation is thin by photosynthetic bacteria, nitrobacteria, denitrification
Bacterium, bacillus subtilis, lactobacillus acidophilus, candida utili and actinomyces composition, are counted in mass ratio：Photosynthetic bacteria：Nitrification
Bacterium：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：Actinomyces are 2:2:1:1:1:1:2.
The embodiment of the present invention provides a kind of preparation method of compound micro-ecological preparation for the black smelly water harnessing in river, is used for
Above-mentioned compound micro-ecological preparation is prepared, is included the following steps：
By photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：
The mass ratio of actinomyces is 2:2:1:1:1:1:2 take respectively photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis,
Lactobacillus acidophilus, candida utili and actinomyces, by the photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis
Bacterium, lactobacillus acidophilus, candida utili and actinomyces are compounded, and after mixing, obtain the compound micro-ecological preparation.
In above-mentioned preparation method, photosynthetic bacteria used is made in the following ways, including：
A. seed liquor is made：Purified photosynthetic bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/
Min, intensity of illumination cultivate 72h for 3000 1x, obtain seed liquor；Wherein, the fluid nutrient medium is what is prepared in following ratios
Mixed liquor：Ammonium chloride 1g/L, sodium acetate 3.5g/L, magnesium chloride 0.1g/L, calcium chloride 0.1g/L, potassium dihydrogen phosphate 0.6g/L, phosphorus
Sour hydrogen dipotassium 0.4g/L, yeast extract 0.1g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min；
B. fermenting and producing：Seed liquor obtained in step a 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium forms, condition of culture and training in the step a identical with the fluid nutrient medium in the seed liquor in the step a
The condition of supporting is identical, and fermentation 72h is to get to photosynthetic bacteria liquid.
In above-mentioned preparation method, nitrobacteria used is made in the following ways, including：
C. seed liquor is made：Purified nitrobacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/
Min cultivates 72h, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Ammonium sulfate 1g/L, phosphoric acid
Hydrogen dipotassium 1g/L, sodium chloride 0.3g/L, magnesium sulfate 0.3g/L, ferrous sulfate 0.03g/L, pH value 7.5-8.0,121 DEG C sterilize
20min；
D. fermenting and producing：Seed liquor obtained in the step c 5% is inoculated into fermentation medium by volume, institute
It is identical with the fluid nutrient medium of the seed liquor in the step c to state fermentation medium composition, in condition of culture and the step c
Condition of culture is identical, and fermentation 72h is to get to nitrobacteria bacterium solution.
In above-mentioned preparation method, denitrifying bacteria used is made in the following ways, including：
E. seed liquor is made：Purified denitrifying bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 100r/
Min is cultivated for 24 hours, obtains seed liquor；Wherein, the fluid nutrient medium is the mixed liquor prepared in following ratios：Sodium acetate 2g/L,
Sodium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.2g/L, micro- 2mL/L, pH value 6.8-7.2,121 DEG C of sterilizings
20min；The trace element is：EDTA 2.06g/L, ferrous sulfate 1.54g/L, manganese chloride 0.2g/L, zinc sulfate 0.1g/L,
Copper sulphate 0.02g/L, sodium manganate 0.1g/L, cobalt chloride 2mg/L, pH value 6.8-7.2；
F. fermenting and producing：Seed liquor obtained in step e 5% is inoculated into fermentation medium by volume, the hair
The composition of ferment culture medium is identical with the fluid nutrient medium of the seed liquor in the step e, condition of culture and the training in the step e
The condition of supporting is identical, and fermentation is for 24 hours to get to denitrifying bacteria bacterium solution.
In above-mentioned preparation method, bacillus subtilis used is made in the following ways, including：
G. seed liquor is made：Purified bacillus subtilis single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C,
200r/min cultivates 18h, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Tryptone
10g/L, yeast extract 5g/L, sodium chloride 10g/L, pH value 7.0-7.5,121 DEG C of sterilizing 20min；
H. fermenting and producing：Seed liquor obtained in the step g 5% is inoculated into fermentation medium by volume, institute
The composition for stating fermentation medium is identical with the fluid nutrient medium of the seed liquor in the step g, in condition of culture and the step g
Condition of culture it is identical, spore rate to be gone out reaches more than 90% stopping fermentation to get to bacillus subtilis bacterium solution.
In above-mentioned preparation method, lactobacillus acidophilus used is made in the following ways, including：
I. seed liquor is made：Purified lactobacillus acidophilus single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C, 100r/
Min, culture for 24 hours, obtain seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Peptone 10g/L, ox
Digested tankage 5g/L, glucose 20g/L, dusty yeast 4g/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.2g/L, citric acid
Three ammonium 2g/L, manganese sulfate 0.05g/L, Tween 80 1mL/L, pH value 7.0-7.5,115 DEG C of sterilizing 20min；
J. fermenting and producing：Seed liquor obtained in the step i 5% is inoculated into fermentation medium by volume, institute
The composition for stating fermentation medium is identical with the fluid nutrient medium of the seed liquor in the step i, in condition of culture and the step i
Condition of culture it is identical, fermentation 18h is to get to lactobacillus acidophilus bacterium solution.
In above-mentioned preparation method, candida utili bacterium used is made in the following ways, including：
K. seed liquor is made：The purified candida utili bacterium single bacterium colony of picking, is inoculated into fluid nutrient medium, 28
DEG C, 200r/min, culture for 24 hours, obtains seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Dusty yeast 10g/L, peptone 20g/L, glucose 20g/
L, pH value 6.5-7.0,115 DEG C of sterilizing 20min.
L. fermenting and producing：The seed liquor of gained in step k 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step k, and condition of culture is same as step k, and fermentation for 24 hours, obtains candida utili
Bacterium bacterium solution.
In above-mentioned preparation method, actinomyces used are made in the following ways, including：
M. seed liquor is made：Purified actinomyces single bacterium colony is taken, is inoculated into fluid nutrient medium, 28 DEG C, 200r/min,
48h is cultivated, obtains seed liquor；Wherein, the culture medium is the mixing prepared in following ratios：Liquid soluble starch 20g/L, chlorine
Change sodium 0.5g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, ferrous sulfate 0.01g/L, pH value 7.0-
7.5,121 DEG C of sterilizing 20min；
N. fermenting and producing：The seed liquor of gained in the step m is inoculated into according to volume ratio 5% in fermentation medium,
The composition of the fermentation medium is identical with the fluid nutrient medium of the seed liquor in the step m, condition of culture and the step m
In condition of culture it is identical, fermentation 48h is to get to actinomyces bacterium solution.
The application method of above-mentioned compound micro-ecological preparation is as follows：
For the improvement of the black smelly water in river, splash a concentration of 10-15g/m2, spilled once within every two days.
The embodiment of the present invention is specifically described in further detail below.
Embodiment 1：
The present embodiment provides a kind of compound micro-ecological preparation for the black smelly water harnessing in river, active constituent (presses quality
Than) be：Photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：Unwrapping wire
Bacterium=2:2:1:1:1:1:2.Specific preparation process is as described below：
The preparation of photosynthetic bacteria：
A. seed liquor is made：The purified photosynthetic bacteria single bacterium colony of picking, is inoculated into fluid nutrient medium, 30 DEG C, 200r/
Min, 3000 1x of intensity of illumination cultivate 72h, obtain seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Ammonium chloride 1g/L, sodium acetate 3.5g/L, magnesium chloride
0.1g/L, calcium chloride 0.1g/L, potassium dihydrogen phosphate 0.6g/L, dipotassium hydrogen phosphate 0.4g/L, yeast extract 0.1g/L, pH value 7.5-
8.0,121 DEG C of sterilizing 20min.
B. fermenting and producing：The seed liquor of gained in step a 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step a, and condition of culture is same as step a, and ferment 72h, obtains photosynthetic bacteria liquid.
The preparation of nitrobacteria：
C. seed liquor is made：The purified nitrobacteria single bacterium colony of picking, is inoculated into fluid nutrient medium, 30 DEG C, 200r/
Min cultivates 72h, obtains seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Ammonium sulfate 1g/L, dipotassium hydrogen phosphate 1g/L, sodium chloride
0.3g/L, magnesium sulfate 0.3g/L, ferrous sulfate 0.03g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min.
D. fermenting and producing：The seed liquor of gained in step c 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step c, and condition of culture is same as step c, and ferment 72h, obtains nitrobacteria bacterium solution.
The preparation of denitrifying bacteria：
E. seed liquor is made：The purified denitrifying bacteria single bacterium colony of picking, is inoculated into fluid nutrient medium, 30 DEG C,
100r/min is cultivated for 24 hours, obtains seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Sodium acetate 2g/L, sodium nitrate 1g/L, dipotassium hydrogen phosphate
0.5g/L, magnesium sulfate 0.2g/L, micro- 2mL/L, pH value 6.8-7.2,121 DEG C of sterilizing 20min.
It is wherein micro-：EDTA 2.06g/L, ferrous sulfate 1.54g/L, manganese chloride 0.2g/L, zinc sulfate 0.1g/L,
Copper sulphate 0.02g/L, sodium manganate 0.1g/L, cobalt chloride 2mg/L, pH value 6.8-7.2.
F. fermenting and producing：The seed liquor of gained in step e 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step e, and condition of culture is same as step e, and fermentation for 24 hours, obtains denitrifying bacteria bacterium
Liquid.
The preparation of bacillus subtilis：
G. seed liquor is made：The purified bacillus subtilis single bacterium colony of picking, is inoculated into fluid nutrient medium, 37 DEG C,
200r/min cultivates 18h, obtains seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Tryptone 10g/L, yeast extract 5g/L, sodium chloride
10g/L, pH value 7.0-7.5,121 DEG C of sterilizing 20min.
H. fermenting and producing：The seed liquor of gained in step g 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step g, and condition of culture is same as step g, and spore rate to be gone out reaches more than 90% and stops
Fermentation, obtains bacillus subtilis bacterium solution.
The preparation of lactobacillus acidophilus：
I. seed liquor is made：The purified lactobacillus acidophilus single bacterium colony of picking, is inoculated into fluid nutrient medium, 37 DEG C,
100r/min, culture for 24 hours, obtain seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Peptone 10g/L, powdered beef 5g/L, glucose 20g/
L, dusty yeast 4g/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.2g/L, Triammonium citrate 2g/L, manganese sulfate
0.05g/L, Tween 80 1mL/L, pH value 7.0-7.5,115 DEG C of sterilizing 20min.
J. fermenting and producing：The seed liquor of gained in step i 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step i, and condition of culture is same as step i, and ferment 18h, obtains lactobacillus acidophilus bacterium
Liquid.
The preparation of candida utili bacterium：
K. seed liquor is made：The purified candida utili bacterium single bacterium colony of picking, is inoculated into fluid nutrient medium, 28
DEG C, 200r/min, culture for 24 hours, obtains seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Dusty yeast 10g/L, peptone 20g/L, glucose 20g/
L, pH value 6.5-7.0,115 DEG C of sterilizing 20min.
L. fermenting and producing：The seed liquor of gained in step k 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium is same as the seed liquid culture medium in step k, and condition of culture is same as step k, and fermentation for 24 hours, obtains candida utili
Bacterium bacterium solution.
The preparation of actinomyces：
M. seed liquor is made：The purified actinomyces single bacterium colony of picking, is inoculated into fluid nutrient medium, 28 DEG C, 200r/
Min cultivates 48h, obtains seed liquor.
The culture medium is the mixing prepared in following ratios：Liquid soluble starch 20g/L, sodium chloride 0.5g/L, nitric acid
Potassium 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, ferrous sulfate 0.01g/L, pH value 7.0-7.5,121 DEG C of sterilizings
20min。
N. fermenting and producing：The seed liquor of gained in step m is inoculated into according to volume ratio 5% in fermentation medium, it is described
Fermentation medium is same as the seed liquid culture medium in step m, and condition of culture is same as step m, and ferment 48h, obtains actinomyces bacterium solution.
The preparation of compound micro-ecological preparation：
In mass ratio, photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：It is false to produce protein
Silk yeast：Actinomyces=2:2:1:1:1:1:2, each bacterium solution is uniformly mixed, completes the preparation of compound micro-ecological preparation.
Embodiment 2：
Compound micro-ecological preparation made from the embodiment of the present invention is applied to Tianjin needs the river administered, and chooses
Part section is separated out a 50m using soft enclosure2Closed area, compound micro-ecological preparation of splashing in the region splashes dense
It spends for 10g/m2, spilled once within every two days.Variation of water is observed, and every two days water samplings detect.It is learnt by experimental result,
After adding compound micro-ecological preparation, the 6th smelly phenomenon of darkness has apparent decrease, and water quality transparency increases, water body after 14 days
In COD, ammonia nitrogen, sulfide removal rate be respectively 86.56%, 80.38% and 77.11% (treatment effect is referring to Fig. 1).
Embodiment 3：
Compound micro-ecological preparation made from the embodiment of the present invention is applied to Hubei Province Xianning needs the river administered
Road, selected part section are separated out a 50m using soft enclosure2Closed area, compound microecological system of splashing in the region
Agent, splash a concentration of 10g/m2, spilled once within every two days.Variation of water is observed, and every two days water samplings detect.By testing
As a result it learns, after adding compound micro-ecological preparation, the 8th smelly phenomenon of darkness has apparent decrease, and water quality transparency increases, and 14
The removal rate of COD, ammonia nitrogen, sulfide after it in water body are respectively 88.62%, 82.58% and 79.06% (treatment effect ginseng
See Fig. 2).
Embodiment 4：
Compound micro-ecological preparation made from the embodiment of the present invention is applied to Xinyang City, Henan Province needs the river administered
Road, selected part section are separated out a 50m using soft enclosure2Closed area, compound microecological system of splashing in the region
Agent, splash a concentration of 10g/m2, spilled once within every two days.Variation of water is observed, and every two days water samplings detect.By testing
As a result it learns, after adding compound micro-ecological preparation, the 6th smelly phenomenon of darkness has apparent decrease, and water quality transparency increases, and 14
The removal rate of COD, ammonia nitrogen, sulfide after it in water body are respectively 87.17%, 85.52% and 79.33% (treatment effect ginseng
See Fig. 3).
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art is in the technical scope of present disclosure, the change or replacement that can readily occur in,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Subject to enclosing.
Claims (10)
1. a kind of compound micro-ecological preparation for the black smelly water harnessing in river, which is characterized in that the compound micro-ecological preparation is by light
Bacterium, nitrobacteria, denitrifying bacteria, bacillus subtilis, lactobacillus acidophilus, candida utili and actinomyces composition are closed,
It counts in mass ratio：Photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：
Actinomyces are 2:2:1:1:1:1:2.
2. the preparation method of the compound micro-ecological preparation according to claim 1 for the black smelly water harnessing in river, feature
It is, the photosynthetic bacteria in the compound micro-ecological preparation is made in the following ways, including：
A. seed liquor is made：Purified photosynthetic bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/min, light
72h is cultivated for 3000 1x according to intensity, obtains seed liquor；Wherein, the fluid nutrient medium is the mixing prepared in following ratios
Liquid：Ammonium chloride 1g/L, sodium acetate 3.5g/L, magnesium chloride 0.1g/L, calcium chloride 0.1g/L, potassium dihydrogen phosphate 0.6g/L, phosphoric acid hydrogen
Dipotassium 0.4g/L, yeast extract 0.1g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min；
B. fermenting and producing：Seed liquor obtained in step a 5% is inoculated into fermentation medium by volume, the fermentation training
It supports base and forms, condition of culture and culture item in the step a identical with the fluid nutrient medium in the seed liquor in the step a
Part is identical, and fermentation 72h is to get to photosynthetic bacteria liquid；
Nitrobacteria in the compound micro-ecological preparation is made in the following ways, including：
C. seed liquor is made：Purified nitrobacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/min trainings
72h is supported, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Ammonium sulfate 1g/L, phosphoric acid hydrogen two
Potassium 1g/L, sodium chloride 0.3g/L, magnesium sulfate 0.3g/L, ferrous sulfate 0.03g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min；
D. fermenting and producing：Seed liquor obtained in the step c 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium forms, condition of culture and culture in the step c identical with the fluid nutrient medium of the seed liquor in the step c
Condition is identical, and fermentation 72h is to get to nitrobacteria bacterium solution；
Denitrifying bacteria in the compound micro-ecological preparation is made in the following ways, including：
E. seed liquor is made：Purified denitrifying bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 100r/min
Culture for 24 hours, obtains seed liquor；Wherein, the fluid nutrient medium is the mixed liquor prepared in following ratios：Sodium acetate 2g/L, nitre
Sour sodium 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.2g/L, micro- 2mL/L, pH value 6.8-7.2,121 DEG C of sterilizings
20min；The trace element is：EDTA 2.06g/L, ferrous sulfate 1.54g/L, manganese chloride 0.2g/L, zinc sulfate 0.1g/L,
Copper sulphate 0.02g/L, sodium manganate 0.1g/L, cobalt chloride 2mg/L, pH value 6.8-7.2；
F. fermenting and producing：Seed liquor obtained in step e 5% is inoculated into fermentation medium by volume, the fermentation training
The composition for supporting base is identical with the fluid nutrient medium of the seed liquor in the step e, condition of culture and the culture item in the step e
Part is identical, and fermentation is for 24 hours to get to denitrifying bacteria bacterium solution；
Bacillus subtilis in the compound micro-ecological preparation is made in the following ways, including：
G. seed liquor is made：Purified bacillus subtilis single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C, 200r/
Min cultivates 18h, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Tryptone 10g/L,
Yeast extract 5g/L, sodium chloride 10g/L, pH value 7.0-7.5,121 DEG C of sterilizing 20min；
H. fermenting and producing：Seed liquor obtained in the step g 5% is inoculated into fermentation medium by volume, the hair
The composition of ferment culture medium is identical with the fluid nutrient medium of the seed liquor in the step g, condition of culture and the training in the step g
The condition of supporting is identical, and spore rate to be gone out reaches more than 90% stopping fermentation to get to bacillus subtilis bacterium solution；
Lactobacillus acidophilus in the compound micro-ecological preparation is made in the following ways, including：
I. seed liquor is made：Purified lactobacillus acidophilus single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C, 100r/min,
Culture for 24 hours, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Peptone 10g/L, powdered beef
5g/L, glucose 20g/L, dusty yeast 4g/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.2g/L, Triammonium citrate
2g/L, manganese sulfate 0.05g/L, Tween 80 1mL/L, pH value 7.0-7.5,115 DEG C of sterilizing 20min；
J. fermenting and producing：Seed liquor obtained in the step i 5% is inoculated into fermentation medium by volume, the hair
The composition of ferment culture medium is identical with the fluid nutrient medium of the seed liquor in the step i, condition of culture and the training in the step i
The condition of supporting is identical, and fermentation 18h is to get to lactobacillus acidophilus bacterium solution；
Candida utili bacterium in the compound micro-ecological preparation is made in the following ways, including：
K. seed liquor is made：The purified candida utili bacterium single bacterium colony of picking, is inoculated into fluid nutrient medium, 28 DEG C,
200r/min, culture for 24 hours, obtain seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Dusty yeast 10g/L, peptone 20g/L, glucose 20g/L, pH
Value 6.5-7.0,115 DEG C of sterilizing 20min.
L. fermenting and producing：The seed liquor of gained in step k 5% is inoculated into fermentation medium by volume, the fermentation training
Foster base is same as the seed liquid culture medium in step k, and condition of culture is same as step k, and fermentation for 24 hours, obtains candida utili bacterium bacterium
Liquid；
Actinomyces in the compound micro-ecological preparation are made in the following ways, including：
M. seed liquor is made：Purified actinomyces single bacterium colony is taken, is inoculated into fluid nutrient medium, 28 DEG C, 200r/min, culture
48h obtains seed liquor；Wherein, the culture medium is the mixing prepared in following ratios：Liquid soluble starch 20g/L, sodium chloride
0.5g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, ferrous sulfate 0.01g/L, pH value 7.0-7.5,
121 DEG C of sterilizing 20min；
N. fermenting and producing：The seed liquor of gained in the step m is inoculated into according to volume ratio 5% in fermentation medium, it is described
The composition of fermentation medium is identical with the fluid nutrient medium of the seed liquor in the step m, in condition of culture and the step m
Condition of culture is identical, and fermentation 48h is to get to actinomyces bacterium solution.
3. the preparation method of a kind of compound micro-ecological preparation for the black smelly water harnessing in river, which is characterized in that be used to prepare power
Profit requires the compound micro-ecological preparation described in 1 or 2, includes the following steps：
By photosynthetic bacteria：Nitrobacteria：Denitrifying bacteria：Bacillus subtilis：Lactobacillus acidophilus：Candida utili：Unwrapping wire
The mass ratio of bacterium is 2:2:1:1:1:1:2 take photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis, acidophilus respectively
Lactobacillus, candida utili and actinomyces, by the photosynthetic bacteria, nitrobacteria, denitrifying bacteria, bacillus subtilis,
Lactobacillus acidophilus, candida utili and actinomyces are compounded, and after mixing, obtain the compound micro-ecological preparation.
4. the preparation method of the compound micro-ecological preparation according to claim 3 for the black smelly water harnessing in river, feature
It is, the photosynthetic bacteria is made in the following ways, including：
A. seed liquor is made：Purified photosynthetic bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/min, light
72h is cultivated for 3000 1x according to intensity, obtains seed liquor；Wherein, the fluid nutrient medium is the mixing prepared in following ratios
Liquid：Ammonium chloride 1g/L, sodium acetate 3.5g/L, magnesium chloride 0.1g/L, calcium chloride 0.1g/L, potassium dihydrogen phosphate 0.6g/L, phosphoric acid hydrogen
Dipotassium 0.4g/L, yeast extract 0.1g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min；
B. fermenting and producing：Seed liquor obtained in step a 5% is inoculated into fermentation medium by volume, the fermentation training
It supports base and forms, condition of culture and culture item in the step a identical with the fluid nutrient medium in the seed liquor in the step a
Part is identical, and fermentation 72h is to get to photosynthetic bacteria liquid.
5. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river, special
Sign is that the nitrobacteria is made in the following ways, including：
C. seed liquor is made：Purified nitrobacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 200r/min trainings
72h is supported, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Ammonium sulfate 1g/L, phosphoric acid hydrogen two
Potassium 1g/L, sodium chloride 0.3g/L, magnesium sulfate 0.3g/L, ferrous sulfate 0.03g/L, pH value 7.5-8.0,121 DEG C of sterilizing 20min；
D. fermenting and producing：Seed liquor obtained in the step c 5% is inoculated into fermentation medium by volume, the hair
Ferment culture medium forms, condition of culture and culture in the step c identical with the fluid nutrient medium of the seed liquor in the step c
Condition is identical, and fermentation 72h is to get to nitrobacteria bacterium solution.
6. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river, special
Sign is that the denitrifying bacteria is made in the following ways, including：
E. seed liquor is made：Purified denitrifying bacteria single bacterium colony is taken, is inoculated into fluid nutrient medium, 30 DEG C, 100r/min
Culture for 24 hours, obtains seed liquor；Wherein, the fluid nutrient medium is the mixed liquor prepared in following ratios：Sodium acetate 2g/L, nitre
Sour sodium 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.2g/L, micro- 2mL/L, pH value 6.8-7.2,121 DEG C of sterilizings
20min；The trace element is：EDTA 2.06g/L, ferrous sulfate 1.54g/L, manganese chloride 0.2g/L, zinc sulfate 0.1g/L,
Copper sulphate 0.02g/L, sodium manganate 0.1g/L, cobalt chloride 2mg/L, pH value 6.8-7.2；
F. fermenting and producing：Seed liquor obtained in step e 5% is inoculated into fermentation medium by volume, the fermentation training
The composition for supporting base is identical with the fluid nutrient medium of the seed liquor in the step e, condition of culture and the culture item in the step e
Part is identical, and fermentation is for 24 hours to get to denitrifying bacteria bacterium solution.
7. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river, special
Sign is that the bacillus subtilis is made in the following ways, including：
G. seed liquor is made：Purified bacillus subtilis single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C, 200r/
Min cultivates 18h, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Tryptone 10g/L,
Yeast extract 5g/L, sodium chloride 10g/L, pH value 7.0-7.5,121 DEG C of sterilizing 20min；
H. fermenting and producing：Seed liquor obtained in the step g 5% is inoculated into fermentation medium by volume, the hair
The composition of ferment culture medium is identical with the fluid nutrient medium of the seed liquor in the step g, condition of culture and the training in the step g
The condition of supporting is identical, and spore rate to be gone out reaches more than 90% stopping fermentation to get to bacillus subtilis bacterium solution.
8. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river, special
Sign is that the lactobacillus acidophilus is made in the following ways, including：
I. seed liquor is made：Purified lactobacillus acidophilus single bacterium colony is taken, is inoculated into fluid nutrient medium, 37 DEG C, 100r/min,
Culture for 24 hours, obtains seed liquor；Wherein, the culture medium is the mixed liquor prepared in following ratios：Peptone 10g/L, powdered beef
5g/L, glucose 20g/L, dusty yeast 4g/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.2g/L, Triammonium citrate
2g/L, manganese sulfate 0.05g/L, Tween 80 1mL/L, pH value 7.0-7.5,115 DEG C of sterilizing 20min；
J. fermenting and producing：Seed liquor obtained in the step i 5% is inoculated into fermentation medium by volume, the hair
The composition of ferment culture medium is identical with the fluid nutrient medium of the seed liquor in the step i, condition of culture and the training in the step i
The condition of supporting is identical, and fermentation 18h is to get to lactobacillus acidophilus bacterium solution.
9. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river, special
Sign is that the candida utili bacterium is made in the following ways, including：
K. seed liquor is made：The purified candida utili bacterium single bacterium colony of picking, is inoculated into fluid nutrient medium, 28 DEG C,
200r/min, culture for 24 hours, obtain seed liquor.
The culture medium is the mixed liquor prepared in following ratios：Dusty yeast 10g/L, peptone 20g/L, glucose 20g/L, pH
Value 6.5-7.0,115 DEG C of sterilizing 20min.
L. fermenting and producing：The seed liquor of gained in step k 5% is inoculated into fermentation medium by volume, the fermentation training
Foster base is same as the seed liquid culture medium in step k, and condition of culture is same as step k, and fermentation for 24 hours, obtains candida utili bacterium bacterium
Liquid.
10. the preparation method of the compound micro-ecological preparation according to claim 3 or 4 for the black smelly water harnessing in river,
It being characterized in that, the actinomyces are made in the following ways, including：
M. seed liquor is made：Purified actinomyces single bacterium colony is taken, is inoculated into fluid nutrient medium, 28 DEG C, 200r/min, culture
48h obtains seed liquor；Wherein, the culture medium is the mixing prepared in following ratios：Liquid soluble starch 20g/L, sodium chloride
0.5g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, ferrous sulfate 0.01g/L, pH value 7.0-7.5,
121 DEG C of sterilizing 20min；
N. fermenting and producing：The seed liquor of gained in the step m is inoculated into according to volume ratio 5% in fermentation medium, it is described
The composition of fermentation medium is identical with the fluid nutrient medium of the seed liquor in the step m, in condition of culture and the step m
Condition of culture is identical, and fermentation 48h is to get to actinomyces bacterium solution.
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| CN109370954A (en) * | 2018-12-07 | 2019-02-22 | 四川清和科技有限公司 | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method |
| CN110205269A (en) * | 2019-06-04 | 2019-09-06 | 广东子辉生态环境科技有限公司 | A kind of Tiny ecosystem flora and its application method and application for repairing artificial swamp |
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| CN109370954A (en) * | 2018-12-07 | 2019-02-22 | 四川清和科技有限公司 | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method |
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| CN110372105B (en) * | 2019-07-11 | 2023-01-17 | 中国科学院烟台海岸带研究所 | Compound microbial agent for improving aquaculture water and preparation method thereof |
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| CN111925955A (en) * | 2020-07-02 | 2020-11-13 | 河海大学 | Composite solid particles prepared from autotrophic and heterotrophic denitrifying bacteria and preparation method thereof |
| CN112322520A (en) * | 2020-10-21 | 2021-02-05 | 广州户户通科技发展有限公司 | Microbial agent for sewage treatment and preparation method thereof |
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| CN112573669A (en) * | 2020-11-20 | 2021-03-30 | 广州绵源环境技术有限公司 | Complex microbial inoculant |
| CN113817651A (en) * | 2021-10-26 | 2021-12-21 | 广东中微环保生物科技有限公司 | Deodorizing composite bacterial agent and deodorant and deodorizing biological liquid thereof |
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Effective date of registration: 20220929 Address after: 100081 Beijing city Haidian District North Shimonoseki street, No. 7 Patentee after: BEIJING SOUND ENVIRONMENT ENGINEERING Co.,Ltd. Patentee after: SANGDE ECOLOGICAL TECHNOLOGY CO.,LTD. Address before: 300456-101, unit 2, building 3, Haifeng logistics park, 600 Luoyang Road, Tianjin Binhai New Area Free Trade Zone (Dongjiang Bonded Port Area) Patentee before: SANGDE ECOLOGICAL TECHNOLOGY CO.,LTD. |