CN108827951A - 一种尿碘定量检测试剂盒 - Google Patents
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- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 title claims abstract description 106
- 229910052740 iodine Inorganic materials 0.000 title claims abstract description 106
- 239000011630 iodine Substances 0.000 title claims abstract description 106
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 86
- 210000002700 urine Anatomy 0.000 title claims abstract description 62
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 239000003054 catalyst Substances 0.000 claims abstract description 14
- 239000003755 preservative agent Substances 0.000 claims abstract description 9
- 230000002335 preservative effect Effects 0.000 claims abstract description 9
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical group [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 12
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 claims description 6
- 229940093930 potassium iodate Drugs 0.000 claims description 6
- 235000006666 potassium iodate Nutrition 0.000 claims description 6
- 239000001230 potassium iodate Substances 0.000 claims description 6
- 150000001263 acyl chlorides Chemical class 0.000 claims description 4
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical group [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- PFUVRDFDKPNGAV-UHFFFAOYSA-N sodium peroxide Chemical compound [Na+].[Na+].[O-][O-] PFUVRDFDKPNGAV-UHFFFAOYSA-N 0.000 claims description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical group [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 3
- 229940033663 thimerosal Drugs 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 13
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 abstract description 6
- 230000029087 digestion Effects 0.000 abstract description 5
- 229910001870 ammonium persulfate Inorganic materials 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- HJTAZXHBEBIQQX-UHFFFAOYSA-N 1,5-bis(chloromethyl)naphthalene Chemical compound C1=CC=C2C(CCl)=CC=CC2=C1CCl HJTAZXHBEBIQQX-UHFFFAOYSA-N 0.000 abstract 1
- GOLCXWYRSKYTSP-UHFFFAOYSA-N arsenic trioxide Inorganic materials O1[As]2O[As]1O2 GOLCXWYRSKYTSP-UHFFFAOYSA-N 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 1
- 229910052684 Cerium Inorganic materials 0.000 description 4
- -1 cerium ions Chemical class 0.000 description 4
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 4
- 210000001685 thyroid gland Anatomy 0.000 description 4
- 206010067997 Iodine deficiency Diseases 0.000 description 3
- TUFLVBXBZSNFCZ-UHFFFAOYSA-N arsenic cerium Chemical compound [As].[Ce] TUFLVBXBZSNFCZ-UHFFFAOYSA-N 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 235000006479 iodine deficiency Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000007800 oxidant agent Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- HVYWMOMLDIMFJA-UHFFFAOYSA-N 3-cholesterol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 HVYWMOMLDIMFJA-UHFFFAOYSA-N 0.000 description 1
- 206010012289 Dementia Diseases 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 208000024799 Thyroid disease Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 208000021510 thyroid gland disease Diseases 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
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Abstract
本发明提供了一种尿碘定量检测试剂盒，一种尿碘定量检测试剂盒，所述试剂盒内的检测试剂由尿碘试剂R1，尿碘试剂R2和尿碘校准品组成；所述尿碘试剂R1由以下组分组成：浓硫酸0.1‑1.50mol/L，过硫酸铵0.5‑2.0mol/L，氯化钠10‑20mmol/L，三氧化二砷20‑50mmol/L，催化剂Ⅰ 0.01‑0.05mol/L，防腐剂0.05％。本发明所述的一种尿碘定量检测试剂盒适用于全自动生化分析仪的检测使用，样本无需提前进行消化处理，可直接用全自动生化分析仪进行测定，样本测定时间仅需10min，大大缩短了检测时间，提高了检测效率。
Description
技术领域
本发明涉及生物技术领域，具体涉及一种尿碘定量检测试剂盒。
背景技术
碘是合成甲状腺急速的必须微量元素，机体摄碘不足或过多都将影响甲状腺功能，造成甲状腺的损伤，导致甲状腺疾病的发生。肾脏是碘的主要排泄器官，且碘90％经尿排出，10％经粪便排出。尿碘水平是直接评价人体碘营养状况的重要指标。尿碘的订量测定为诊断人们是否缺碘、碘摄入是否过量提供了重要的科学依据。
妊娠期缺碘会使得孕妇与胎儿甲状腺合成减少，婴幼儿的体格和智能发育受到孕妇碘营养水平的直接影响。所以孕妇尿碘水平能够有效反映孕妇碘摄入量和新生儿的碘营养状况。孕妇科学合理补碘，切忌盲目补碘。孕妇补碘的重要时期是妊娠前3个月内，由于胚胎早期补碘能够促使儿童的智力发育，预防婴幼儿发生克汀病。妊娠5个月后补碘难，以防止婴幼儿发生智力缺陷。
目前尿碘测定被国内外采用的技术有砷铈催化分光光度法，等离子色谱质谱法和化学发光法，这些方法都需要对样本提前进行消化处理，且消化时间长，耗时长。
发明内容
有鉴于此，本发明旨在提出一种尿碘定量检测试剂盒，以延长小而密低密度脂蛋白胆固醇检测试剂盒的有效时间，增强稳定性。
为达到上述目的，本发明的技术方案是这样实现的：
一种尿碘定量检测试剂盒，所述试剂盒内的检测试剂由尿碘试剂R1，尿碘试剂R2和尿碘校准品组成，所述尿碘试剂R1，尿碘试剂R2和尿碘校准品的组成成分以及浓度如下：
尿碘试剂R1
尿碘试剂R2
尿碘校准品
防腐剂 0.5％，
碘酸钾纯品。
进一步的，所述尿碘试剂R1中的催化剂Ⅰ为过氧化酰氯或过氧化氢钠中的一种，所述尿碘试剂R2中的催化剂Ⅱ为次氯酸钠。
进一步的，所述尿碘试剂R1中的催化剂Ⅰ浓度为0.02mol/L，所述尿碘试剂R2中的催化剂Ⅱ浓度为0.5mol/L。
进一步的，所述尿碘试剂R1和尿碘试剂R2中的防腐剂均为硫柳汞。
进一步的，所述尿碘校准品中的防腐剂为叠氮钠。
相对于现有技术，本发明所述的一种尿碘定量检测试剂盒具有以下优势：
本发明所述的一种尿碘定量检测试剂盒的检测试剂由尿碘试剂R1，尿碘试剂R2和尿碘校准品组成，适用于全自动生化分析仪的检测使用，样本无需提前进行消化处理，可直接用全自动生化分析仪进行测定，样本测定时间仅需10min，大大缩短了检测时间，提高了检测效率。
附图说明
图1为本发明实施例1所述的一种尿碘定量检测试剂盒试剂的线性关系图；
图2为本发明实施例2所述的一种尿碘定量检测试剂盒试剂的线性关系图。
具体实施方式
除有定义外，以下实施例中所用的技术术语具有与本发明所属领域技术人员普遍理解的相同含义。以下实施例中所用的试验试剂，如无特殊说明，均为常规生化试剂；所述实验方法，如无特殊说明，均为常规方法。
下面结合实施例来详细说明本发明。
本发明所述的一种尿碘定量检测试剂盒的检测原理为：
本发明的试剂盒是利用强氧化剂过硫酸铵在催化剂过氧化酰氯的作用下将尿液样本中的有机碘迅速消解为无机碘，无需对样本进行前处理，可节约检测时间，优化检测流程。
2Ce4++2I-→2Ce3++2I2 I2+As3+→2I-+As5+
尿样经过强氧化剂过硫酸铵的消化后，使有机碘消化成无机碘，然后在酸性环境中，利用碘对砷铈氧化还原反应的催化作用，反应中黄色的4价铈离子被还原成无色的3价铈离子，碘含量越高，反应速率越快，动态监测黄色4价铈离子被还原成无色的3价铈离子的反应速率，可得到碘含量。
本发明所述的一种尿碘定量检测试剂盒，所述试剂盒内的检测试剂由尿碘试剂R1，尿碘试剂R2和尿碘校准品组成，所述尿碘试剂R1，尿碘试剂R2和尿碘校准品的组成成分以及浓度如下：
尿碘试剂R1
尿碘试剂R2
尿碘校准品
防腐剂 0.5％，
碘酸钾纯品。
所述尿碘试剂R1中的催化剂Ⅰ为过氧化酰氯或过氧化氢钠中的一种，所述尿碘试剂R2中的催化剂Ⅱ为次氯酸钠；所述尿碘试剂R1中的催化剂Ⅰ浓度为0.02mol/L，所述尿碘试剂R2中的催化剂Ⅱ浓度为0.5mol/L。
所述尿碘试剂R1和尿碘试剂R2中的防腐剂均为硫柳汞；所述尿碘校准品中的防腐剂为叠氮钠。
本发明所述的一种尿碘定量检测试剂盒的使用方法为：
1)对全自动生化分析仪参数设定，基本参数为：
2)检验方法：
3)计算结果：
实施例1
一种尿碘定量检测试剂盒的液体双试剂具体成分如下：
尿碘试剂R1
尿碘试剂R2
尿碘校准品
叠氮钠 0.5％，
碘酸钾纯品。
本实施例试剂采用罗氏C311全自动生化分析仪，对照方法为WST107-1999尿碘大的砷铈催化分光光度测定方法，同时对20份样本按照各自的参数进行测定，并对测定值进行相关分析，结果见图1，X轴代表的是本发明试剂的测定值，Y轴表示的是国标方法的测定值。相关系数：r2＝0.9984，线性方程为y＝1.0323x-5.629，结果表明本实施例试剂与国标方法良好的相关性。
实施例2
尿碘试剂R1
尿碘试剂R2
尿碘校准品
叠氮钠 0.5％，
碘酸钾纯品。
选择一个UI值约为1000ug/l的高值样本，分别稀释成不同的浓度，并用本实例所述的试剂进行线性验证，其线性范围为5-1000ug/l,线性范围验证结果见图2，相关系数为0.9993，本实施例试剂的线性范围可达到5-1000ug/l。
实施例3
尿碘试剂R1
尿碘试剂R2
尿碘校准品
叠氮钠 0.5％，
碘酸钾纯品。
用本实例试剂进行精密度验证，对同一份样本连续测定20次，计算其CV值，根据表1结果，本发明试剂的精密度控制在5％范围内，具有良好的精密性。
表1精密度试验结果
| 样本号 | 测定结果 |
| 1 | 165 |
| 2 | 167 |
| 3 | 159 |
| 4 | 153 |
| 5 | 152 |
| 6 | 154 |
| 7 | 152 |
| 8 | 154 |
| 9 | 159 |
| 10 | 161 |
| 11 | 153 |
| 12 | 154 |
| 13 | 155 |
| 14 | 153 |
| 15 | 157 |
| 16 | 158 |
| 17 | 155 |
| 18 | 154 |
| 19 | 153 |
| 20 | 151 |
| 均值 | 155.95 |
| CV | 2.7％ |
由以上实施例可知，本发明所述的一种尿碘定量检测试剂盒，检测速度快，准确高，有良好的精密度和线性，适用于临床的快速检测，能满足临床检验需要。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (5)
1.一种尿碘定量检测试剂盒，其特征在于：所述试剂盒内的检测试剂由尿碘试剂R1，尿碘试剂R2和尿碘校准品组成，所述尿碘试剂R1，尿碘试剂R2和尿碘校准品的组成成分以及浓度如下：
尿碘试剂R1
尿碘试剂R2
尿碘校准品
防腐剂 0.5％，
碘酸钾纯品。
2.根据权利要求1所述的一种尿碘定量检测试剂盒，其特征在于：所述尿碘试剂R1中的催化剂Ⅰ为过氧化酰氯或过氧化氢钠中的一种，所述尿碘试剂R2中的催化剂Ⅱ为次氯酸钠。
3.根据权利要求1所述的一种尿碘定量检测试剂盒，其特征在于：所述尿碘试剂R1中的催化剂Ⅰ浓度为0.02mol/L，所述尿碘试剂R2中的催化剂Ⅱ浓度为0.5mol/L。
4.根据权利要求1所述的一种尿碘定量检测试剂盒，其特征在于：所述尿碘试剂R1和尿碘试剂R2中的防腐剂均为硫柳汞。
5.根据权利要求1所述的一种尿碘定量检测试剂盒，其特征在于：所述尿碘校准品中的防腐剂为叠氮钠。
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Citations (5)
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