CN108815013B

CN108815013B - Skin care and whitening stock solution and application thereof in skin permeation promoting technology

Info

Publication number: CN108815013B
Application number: CN201810888403.2A
Authority: CN
Inventors: 唐玮键; 李玉兵; 吴思怡
Original assignee: Shanghai Youcui Biotechnology Co ltd
Current assignee: Shanghai Youcui Biotechnology Co ltd
Priority date: 2018-08-06
Filing date: 2018-08-06
Publication date: 2021-12-03
Anticipated expiration: 2038-08-06
Also published as: CN108815013A

Abstract

The invention discloses a skin-care whitening stock solution, which comprises the following components: glycerin, humectant, sodium hyaluronate, surfactant, solvent, preservative, nicotinamide, tranexamic acid and water. The invention also discloses a preparation method and application thereof in skin penetration technology. The skin-care whitening formula disclosed by the invention has the effects of whitening, nourishing and moisturizing the skin by compounding the components in the formula, and is combined with a skin permeation technology to promote the effects of diminishing inflammation, sterilizing and promoting wound healing in the process of pricking the skin and forming a drug delivery channel, so that the whitening product and the permeation promoting technology are perfectly combined.

Description

Skin care and whitening stock solution and application thereof in skin permeation promoting technology

Technical Field

The invention relates to a whitening product, a preparation method and application thereof, in particular to a skin-care whitening stock solution, a preparation method and application thereof in a skin permeation promoting technology, and belongs to the technical field of daily chemicals.

Background

People have been beautiful for a long time, white skin is the target of all-year-round pursuits of many women, dull skin is yellow skin color and no luster caused by various factors, and the skin color is dull except aging, environmental pollution, bad living habits, mental stress and the like.

The external application method for skin whitening has obvious effect from mercury-containing preparation in 20 th century to hydroquinone in the beginning of 21 st century, but has renal toxicity and carcinogenicity. Kojic acid, arbutin, vitamin C, glycyrrhizic acid and the like achieve the whitening effect mainly by inhibiting tyrosinase, but have side effects such as teratogenesis and the like, the history of treating chloasma and improving skin color by oral tranexamic acid has been more than 30 years, the effect is clear, but side effects such as gastrointestinal discomfort, menses reduction and the like exist. How to safely and effectively solve the problem of dark skin color is still a hot spot at present, and how to seek a safe and effective whitening method is the direction of continuous research of people.

At present, the administration techniques applied to increase the skin permeation and absorption of external drugs or skin care products include iontophoresis, ultrasound, electroporation, microneedles and chemical penetration enhancers, which have more or less disadvantages such as insignificant effects, inconvenient application, high susceptibility to wound infection, etc. while promoting absorption.

In recent years, nano-micro-needles become an emerging skin permeation promoting technology, and researches show that the nano-micro-needles (nano-wafers) can form a drug delivery channel on the surface of the skin in a short time, the channel is naturally closed within about 20min, and the skin recovers continuous integrity. New skin penetration techniques have been widely used in the skin beauty industry, but whitening products that combine with new penetration techniques and promote each other have not kept pace and are not in perfect conformity.

Disclosure of Invention

In order to solve the defects of the prior art, the invention aims to provide the skin care and whitening stock solution which has high conformity with the skin permeation promoting technology, mutual promotion and good effect, the preparation method thereof and the application thereof in the skin permeation technology.

In order to achieve the above object, the present invention adopts the following technical solutions:

the moisturizing agent panthenol or pantothenic acid adopted by the invention has the effects of moisturizing, resisting inflammation, moistening the skin, stimulating cell division and the like, has good biological activity, has hygroscopicity and electrostatic adsorption, can deeply permeate into the skin, stimulates the growth of epithelial cells, promotes wound healing, plays a role in diminishing inflammation, is combined with a skin permeation technology, promotes and strengthens the action effect.

The skin-care whitening lotion provided by the invention comprises sodium hyaluronate, has the effects of diminishing inflammation and accelerating wound healing, can moisten skin and increase luster, and can prevent chapped skin and wrinkles.

The solvent in the skin-care whitening lotion provided by the invention is a humectant with excellent performance, has an antiseptic effect, can be used for preparing a product without an antiseptic, reduces allergy caused by the antiseptic, can improve the water resistance, the dissolution indissolvable active ingredients and the like of a sunscreen product formula, is compounded with the antiseptic octanoyl hydroximic acid in the formula, has enhanced antiseptic performance, and does not have the adverse effect of the traditional antiseptic.

In addition, the preservative ethylhexyl glycerin in the formula of the skin-care whitening liquid can be added into a water phase and an oil phase, when the preservative is added into the water phase, a bacteriostasis environment can be formed, the preservative effect on the whole formula is achieved, the traditional cosmetic preservative such as phenoxyethanol, methylisothiazolinone or methylparaben can be enhanced, the antibacterial effect of alcohols and glycols can be enhanced, the antibacterial effect of the glycols such as pentanediol or octanediol can be improved in an oil-in-water emulsion, and in addition, the ethylhexyl glycerin can effectively inhibit the growth and the propagation of bacteria causing peculiar smell without affecting beneficial skin flora of a human body.

The nicotinamide in the formula can be used as a nutritional additive in cosmetics to prevent rough skin, has the function of promoting the circulation of capillary vessels in hair follicle parts, and provides more nutritional components for hair follicles.

In addition, tranexamic acid is also introduced into the formula of the skin-care whitening lotion. The tranexamic acid has the effects of whitening, fading spots, helping skin to relieve and calm, preventing the skin from generating pigmentation, whitening the skin, inhibiting the catalytic action of protease on the hydrolysis of peptide bonds, preventing the activity of enzymes such as hair-origin protease, inhibiting the disorder of epidermal cell functions of black spots, inhibiting melanin enhancement factor groups, and completely cutting off the generation path of melanin formed by ultraviolet irradiation, namely enabling the black spots not to become thick, enlarge and increase any more, thereby effectively preventing and improving the pigmentation of the skin, and leading plasminogen not to be converted into plasmin by reversibly blocking lysine binding sites on plasminogen molecules, thereby effectively inhibiting the dissolution of fibrin and generating the hemostatic action.

Specifically, the invention provides a skin-care whitening stock solution, and each 100kg of the stock solution comprises the following raw materials:

glycerol: 2-5 kg;

humectant: 1 kg;

sodium hyaluronate: 0.01-1 kg;

surfactant (b): 0.05 kg;

solvent: 1-10 kg;

preservative: 0.01-1 kg;

preservative: 0.05-0.2 kg;

nicotinamide: 0.5-5 kg;

tranexamic acid: 0.1-4 kg;

water: the balance of the weight percentage is as follows,

the water, the glycerin and the humectant are mixed to form phase A, the sodium hyaluronate, the surfactant, the solvent, the preservative and the preservative are mixed to form phase B, and the nicotinamide and the tranexamic acid are classified as phase C.

Further, the humectant is panthenol or pantothenic acid.

The surfactant is disodium EDTA.

The solvent is at least one of hexanediol, pentanediol, propylene glycol and caprylyl glycol.

The preservative is ethylhexyl glycerol.

The preservative is caprylyl hydroximic acid.

The invention also discloses a preparation method of the skin care and whitening stock solution, which comprises the following steps:

s1: firstly, adding the phase A components into a main pot according to the mass ratio, heating to 80 ℃, and uniformly mixing;

s2: cooling the main pot to 50 ℃, adding the components of the phase B into the side pot according to the mass ratio, stirring and mixing uniformly, putting the mixture into the phase A, and stirring and mixing uniformly with the phase A;

s3: and cooling the main pot to 45 ℃ again, finally sequentially adding the C-phase nicotinamide and tranexamic acid into the main pot, stirring and dissolving uniformly, cooling to room temperature, and discharging after the detection is qualified to obtain the skin care and whitening stock solution.

In addition, the application of the skin care and whitening stock solution in the skin permeation promoting technology comprises the following steps: after the skin is cleaned, a nano-chip permeation promoting instrument is vertically contacted with the surface of the skin, the skin is pricked point by point, each point acts for 1s, then the width of one chip is laterally shifted to perform the pricking of the next point, a drug delivery channel is formed on the surface of the skin, skin care and whitening stock solution is introduced while the pricking is performed, and the skin at the same point is repeatedly introduced for three times.

Furthermore, the service cycle of the skin care and whitening stock solution through the skin permeation technology is as follows: once per week. While the depth of the prick is at least the stratum corneum.

The invention has the advantages that: the skin-care whitening formula disclosed by the invention has the effects of whitening, nourishing and moisturizing the skin by compounding the components in the formula, and is combined with a skin permeation technology to promote the effects of diminishing inflammation, sterilizing and promoting wound healing in the process of pricking the skin and forming a drug delivery channel, so that the whitening product and the permeation promoting technology are perfectly combined.

Detailed Description

The advantages and features of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

In the following examples, the humectant is panthenol, but it can also be pantothenic acid, the surfactant is disodium EDTA, the solvent is a mixture of hexylene glycol, pentanediol and propylene glycol, the preservative is ethylhexyl glycerol, and the preservative is caprylhydroxamic acid

Example 1:

every 100kg of the skin care and whitening stock solution comprises the components and contents (unit kg) shown in the following table:

example 2:

example 3:

the skin care and whitening stock solutions of examples 1-3 were prepared by the following methods:

s2: cooling the main pot to 50 ℃, adding the components of the phase B into the side pot according to the mass ratio, stirring uniformly, putting the mixture into the phase A, and stirring and mixing uniformly with the phase A;

The skin care and whitening stock solution described in example 1 and comparative example 1 (0.5% tranexamic acid solution) and comparative example 2 (normal saline) were applied to skin permeation-enhancing technique, and the application process and application conditions were the same.

The method specifically comprises the following steps:

firstly, the data of the testee:

200 healthy volunteers, skin types Fitzpatrick III-IV, ages of 28-55 years and 100 male and female patients are recruited, and the faces of the volunteers are not treated by micro-needles; plain body is healthy, and has no infectious diseases, blood system diseases, mental diseases, skin tumor; no history of skin allergy; women are not in pregnancy or lactation; has no bad taste such as smoking and drinking, and is divided into five groups according to male and female proportion, wherein each group comprises 40 persons, male 20 persons and female 20 persons.

II, instruments and materials:

nano microneedle: the research adopts 3DL-Z type nano micro needles, which are formed by sticking a micro needle array chip on a plastic crystal head and are provided by Suzhou Natong biological nanotechnology limited company. The chip is long (5.0 +/-2.5) mm, wide (5.0 +/-2.5) mm, thick 0.3mm and the number of dot matrixes is (100 +/-25); the array height is 0.15 mm; taper (40 ± 3) °, lot number: 251022-2. Nanocrystalline permeation-promoting instrument (SPE-10, Suzhou Natong biological nanotechnology Co., Ltd.), Germany CK multifunctional skin tester. 0.5% tranexamic acid solution (common name: tranexamic acid sodium chloride injection, trade name: Jiening, size: 100ml, tranexamic acid 0.5g and sodium chloride 0.85g, Chinese medicine standard H20020442, available from Changchun Chengxi pharmaceutical Co., Ltd.)

Thirdly, the treatment method comprises the following steps:

after the face is cleaned and naturally dried, the skin of the whole face of a subject in a normal saline group is continuously wet-coated with normal saline, then an electric nano crystal permeation promoting instrument is used for vertically contacting the surface of the skin and electrically pricking point by point, each point acts for 1s, and the pricking depth is the skin cuticle. Then, the patient moves aside by the width of one chip to perform the next prick, and the whole face is guided in the method, and each treatment is repeated for 3 times. The subjects in the group of 0.5% tranexamic acid solution and the subjects in examples 1 to 3 who had skin care and whitening raw liquids were each treated with 0.5% tranexamic acid solution and the skin care and whitening raw liquids in examples 1 to 3 in the same manner. The five groups of subjects receive treatment once a week for 2 months, and the subjects can not receive other facial beauty treatment and take beauty related medicines or health care products during the treatment period; photographs were taken with a digital camera and the trans-epidermal water loss (TEWL), stratum corneum water content, erythema dose, melanin and skin elasticity were measured before and after each group of subjects received treatment weekly; subject satisfaction and adverse reactions were investigated via a skin status questionnaire.

Fourthly, skin test:

the test chamber has no direct sunlight and no wind, and the room temperature is controlled to be about 22 ℃ and the humidity is controlled to be 50-60 percent. Each subject did not use any cosmetics after cleaning the face and was allowed to sit still in the room 30min before testing. The method comprises the steps of shooting an original picture by using a digital camera, and detecting TEWL, the water content of the stratum corneum, the amount of erythema, melanin and skin elasticity by using a water loss test probe, a water test probe, a melanin and heme test probe and an elastic fiber tissue test probe of a German CK multifunctional skin tester. During testing, keeping the probe of the instrument vertically acting on the skin, selecting four fixed positions of the face, reading after the numerical value is stable each time, and taking an average value to perform statistical analysis; prevent hair and other articles from covering and contacting the part to be measured.

Fifthly, the evaluation standard of curative effect is as follows:

subjective evaluation: subjects were asked to score the skin condition before and after the last treatment. The skin color and glossiness score is from 1 to 5, the score is very unsatisfactory 1, 2, generally 3, 4, 5 and 10, and the subjective score is higher, namely, the skin state is more satisfactory;

objective assessment: the indexes of the German CK multifunctional skin tester are compared with the numerical changes before and after the last treatment.

Sixthly, statistical analysis:

using SPSS software version 17.0, the test data are all x^-S means that P < 0.05 was considered statistically significant using the t-test.

Seventhly, obtaining a result:

(1) MC760 digital skin analyzer test results:

TEWL, stratum corneum water content, erythema level, skin elasticity: see table 1. Before treatment, two groups of volunteers have no statistical difference in skin water loss, stratum corneum water content, erythema index and elasticity index, namely have comparability; after treatment with nano-micro needles with a needle length of 0.15mm, the physiological saline group was tested for the results of 72 hours after the last treatment of the group consisting of transdermal water loss, stratum corneum water content, erythema index, elasticity index, and 0.5% tranexamic acid solution, and the skin care and whitening stock solution described in examples 1-3. There were no statistical differences.

TABLE 1 comparison of TEWL, stratum corneum Water content, erythema index, elasticity index, melanin values measured before and 72h after the last treatment in five groups of volunteers (x)^-±s)

As can be seen from Table 1: the skin treated by the nano-micro needles for 72 hours has no statistical difference in the percutaneous water loss, erythema index, elasticity index and the ratio before treatment, but obviously, the effects of the examples 1 to 3 are better than those of the comparative examples 1 and 2; in addition, the melanin values of comparative example 1 and examples 1 to 3 of the present invention are significantly different from the melanin value before the initial treatment (P < 0.05), and the effects of examples 1 to 3 of the present invention are far superior to those of comparative example 1, in terms of the water content of the horny layer (P < 0.05).

(2) Satisfaction survey results:

the skin states of five groups of volunteers before treatment are respectively divided into (4.600 +/-1.466) and (5.0 +/-1.925), and the skin states are comparable without statistical difference. Comparative example 1 volunteers scored a skin condition after treatment (6.287 ± 1.622) score with no statistical difference compared to before treatment; comparative example 2 volunteers scored (5.244 ± 1.573) for the skin condition after treatment, with no statistical difference compared to before treatment, while volunteers of examples 1-3 scored for the skin condition after treatment, respectively: the scores of (8.468 + -1.975), (9.077 + -0.952), (8.276 + -1.385) are all significantly different from those before treatment (P < 0.05).

All volunteers involved in the study did not have adverse reactions such as infection, erythema and edema.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.

Claims

1. The skin-care whitening stock solution is characterized in that each 100kg of the stock solution comprises the following raw materials:

glycerol: 2-5 kg;

panthenol or pantothenic acid: 1 kg;

sodium hyaluronate: 0.01-1 kg;

surfactant (b): 0.05 kg;

solvent: 1-10kg, wherein the solvent is at least one of hexanediol, pentanediol and propylene glycol;

ethyl hexyl glycerin: 0.01-1 kg;

caprylyl hydroximic acid: 0.05-0.2 kg;

nicotinamide: 0.5-5 kg;

tranexamic acid: 0.1-4 kg;

water: the balance of the weight percentage is as follows,

the water, the glycerin and the humectant are mixed to form phase A, the sodium hyaluronate, the surfactant, the solvent, the preservative and the preservative are mixed to form phase B, the nicotinamide and the tranexamic acid are classified as phase C,

the preparation method of the skin-care whitening stock solution comprises the following steps:

2. The skin care and whitening lotion as defined in claim 1, wherein the surfactant is disodium EDTA.

3. The use of the skin care whitening lotion according to claim 1 in skin penetration enhancing technology, comprising the steps of: after the skin is cleaned, a nano-chip permeation promoting instrument is vertically contacted with the surface of the skin, the skin is pricked point by point, each point acts for 1s, then the width of one chip is laterally shifted to perform the pricking of the next point, a drug delivery channel is formed on the surface of the skin, skin care and whitening stock solution is introduced while the pricking is performed, and the skin at the same point is repeatedly introduced for three times.

4. The application of the skin care and whitening stock solution in the skin permeation promotion technology according to claim 3, wherein the service cycle of the skin care and whitening stock solution through the skin permeation technology is as follows: once per week.

5. The use of a skin care and whitening lotion according to claim 3, wherein the depth of the prickling is at least the stratum corneum.