CN108781850A - A kind of Salt-Tolerance Identification method of the black China fir of hybridization - Google Patents
A kind of Salt-Tolerance Identification method of the black China fir of hybridization Download PDFInfo
- Publication number
- CN108781850A CN108781850A CN201710311235.6A CN201710311235A CN108781850A CN 108781850 A CN108781850 A CN 108781850A CN 201710311235 A CN201710311235 A CN 201710311235A CN 108781850 A CN108781850 A CN 108781850A
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- CN
- China
- Prior art keywords
- salt
- hybridization
- china fir
- tolerance
- identification method
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a kind of Salt-Tolerance Identification methods of the black China fir of hybridization, include the following steps：Using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, it is more several than more consistent material to choose growing way；Equivalent culture substrate is weighed, after measuring substrate water content, above-mentioned experiment material is colonized in plastic flowerpot one by one, is placed in greenhouse and is cultivated；After late May plant survives restoration ecosystem, NaCl Stress treatments are carried out 50-60 days, and totally 5 processing are horizontal by setting 0,3,6,9,12gkg-1, if 4 repetitions；Carry out the measurement of plant height, ground diameter, physical signs such as sodium potassium content of biomass estimation, measuring chlorophyll content, propylene glycol, proline, root, stem and leaf above and below the ground, data are analyzed, evaluate its salt tolerance, can rational evaluation its salt tolerance, accurate data be provided support for the popularizing planting in later stage.
Description
Technical field
The present invention relates to the identification technology field of the black China fir salt tolerance of hybridization, more particularly to the salt tolerance mirror of a kind of black China fir of hybridization
Determine method.
Background technology
Taxodium seeds include 3 kinds of bald cypress, taxodium ascendens and Taxodium mucronatum (referred to as black China fir), originate in North America
And Mexico, the advantages that tool growth is fast, form is straight, material is good, water-fast wet, wide adaptability, in Deposits in Eastern Coastal China and inland basin
Be with a wide range of applications in the afforestation in area and China carry out woody energy one of emphasis seeds.
" the black China fir of hybridization " has huge quantity increment and increment of organism advantage, future Timber stands, the energy in China
Woods, carbon converge woods, leisure woods and forests for water supply conservation construction in terms of have broad application prospects, can be widely used in China's wetland
Restoration of the ecosystem, coast protection forest construction and saline land greening afforestation and township afforestation and farm field and forest network etc..
At this stage, it needs to carry out and form observation, Salt-Tolerance Identification, region is carried out to acquired " the black China fir of hybridization " clone
The work such as experiment and Demonstration afforestation, choose suitable improved seeds, are made with afforesting with meeting our province East Coastal beach saline-alkali
The demand of woods, wherein Salt-Tolerance Identification are the important links in popularizing planting work.
In conclusion how to formulate the Salt-Tolerance Identification method that complete set effectively hybridizes black China fir, its is resistance to for rational evaluation
Salt provides accurate data support for the popularizing planting in later stage, becomes those skilled in the art's technical problem urgently to be resolved hurrily.
Invention content
The object of the present invention is to provide a kind of Salt-Tolerance Identification method of the black China fir of hybridization, can rational evaluation its salt tolerance,
Accurate data support is provided for the popularizing planting in later stage.
In order to solve the above technical problems, the present invention provides following technical solutions：
A kind of Salt-Tolerance Identification method of the black China fir of hybridization, includes the following steps：
(1) it using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, chooses
Growing way is more several than more consistent material；
(2) equivalent culture substrate is weighed, after measuring substrate water content, above-mentioned experiment material is colonized in plastic flowerpot one by one
In, it is placed in greenhouse and is cultivated；
(3) after late May plant survives restoration ecosystem, NaCl Stress treatments are carried out 50-60 days, and setting 0,3,6,9,
Totally 5 processing are horizontal by 12gkg-1, if 4 repetitions；
(4) plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, proline, root are carried out
The measurement of the physical signs such as the sodium potassium content of cauline leaf, analyzes data, evaluates its salt tolerance.
Preferably, placement tray under each flowerpot in the step (2).
Preferably, further include that the solution flowed out when in the step (3) by watering is refunded in flowerpot again.
Preferably, further include the salinity and matrix oozed out in the slave flowerpot accumulated in periodic cleaning pallet and refund former flower
In basin.
The Salt-Tolerance Identification method of the black China fir of hybridization provided by the present invention, carries out Evaluation of Salt Tolerance using pot-culture method, makes every
The growth conditions of strain experiment material is relatively independent, and experimental data easily acquires, with strong points, and accuracy is high, and experiment is using complete
RANDOMIZED BLOCK DESIGN ensures that the data acquisition basis between the district's groups being compared is consistent, and data randomness is strong, ensures data analysis
Accuracy, to reach can rational evaluation its salt tolerance, provide the mesh that accurate data support for the popularizing planting in later stage
's.
Specific implementation mode
Core of the invention is to provide a kind of Salt-Tolerance Identification method of the black China fir of hybridization, can rational evaluation its salt tolerance,
Accurate data support is provided for the popularizing planting in later stage.
The Salt-Tolerance Identification method of the black China fir of hybridization provided by the present invention, includes the following steps：
Step S1, using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted,
It is more several than more consistent material to choose growing way；
Step S2 weighs equivalent culture substrate, and after measuring substrate water content, above-mentioned experiment material is colonized in plastics one by one
In flowerpot, the placement tray under each flowerpot is placed in greenhouse and is cultivated；
Step S3 after late May plant survives restoration ecosystem, carries out NaCl Stress treatments 50-60 days, and setting 0,3,6,
9, totally 5 processing are horizontal by 12gkg-1, if 4 repetitions, during Stress treatment, the solution flowed out when by watering refunds flower again
In basin, and the salinity that oozes out and matrix and refunded in former flowerpot in the slave flowerpot accumulated in periodic cleaning pallet；
Step S4 carries out plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, dried meat ammonia
The measurement of the physical signs such as sour, root, stem and leaf sodium potassium content, analyzes data, evaluates its salt tolerance.
The Salt-Tolerance Identification method of the black China fir of hybridization provided by the present invention, carries out Evaluation of Salt Tolerance using pot-culture method, makes every
The growth conditions of strain experiment material is relatively independent, and experimental data easily acquires, with strong points, and accuracy is high, and experiment is using complete
RANDOMIZED BLOCK DESIGN ensures that the data acquisition basis between the district's groups being compared is consistent, and data randomness is strong, ensures data analysis
Accuracy, to reach can rational evaluation its salt tolerance, provide the mesh that accurate data support for the popularizing planting in later stage
's.
A kind of Salt-Tolerance Identification method of the black China fir of hybridization provided by the present invention is described in detail above.Herein
Applying specific case, principle and implementation of the present invention are described, and the explanation of above example is only intended to help
Understand the method and its core concept of the present invention.It should be pointed out that for those skilled in the art, not taking off
, can be with several improvements and modifications are made to the present invention under the premise of from the principle of the invention, these improvement and modification also fall into this
In invention scope of the claims.
Claims (4)
1. a kind of Salt-Tolerance Identification method of the black China fir of hybridization, which is characterized in that include the following steps：
(1) using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, growing way is chosen
It is more several than more consistent material；
(2) equivalent culture substrate is weighed, after measuring substrate water content, above-mentioned experiment material is colonized in plastic flowerpot one by one,
It is placed in greenhouse and is cultivated；
(3) after late May plant survives restoration ecosystem, NaCl Stress treatments are carried out 50-60 days, and setting 0,3,6,9,12g
Totally 5 processing are horizontal by kg-1, if 4 repetitions；
(4) plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, proline, root, stem and leaf are carried out
The physical signs such as sodium potassium content measurement, data are analyzed, its salt tolerance is evaluated.
2. the Salt-Tolerance Identification method of the black China fir of hybridization as described in claim 1, which is characterized in that each in the step (2)
Placement tray under the flowerpot.
3. the Salt-Tolerance Identification method of the black China fir of hybridization as claimed in claim 2, which is characterized in that further include, in the step
(3) solution flowed out when in by watering is refunded in flowerpot again.
4. the Salt-Tolerance Identification method of the black China fir of hybridization as claimed in claim 2, which is characterized in that further include periodic cleaning support
The salinity and matrix that are oozed out in the slave flowerpot accumulated in disk are simultaneously refunded in former flowerpot.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710311235.6A CN108781850A (en) | 2017-05-05 | 2017-05-05 | A kind of Salt-Tolerance Identification method of the black China fir of hybridization |
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|---|---|---|---|
| CN201710311235.6A CN108781850A (en) | 2017-05-05 | 2017-05-05 | A kind of Salt-Tolerance Identification method of the black China fir of hybridization |
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| CN108781850A true CN108781850A (en) | 2018-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710311235.6A Pending CN108781850A (en) | 2017-05-05 | 2017-05-05 | A kind of Salt-Tolerance Identification method of the black China fir of hybridization |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110320208A (en) * | 2019-08-09 | 2019-10-11 | 山东省农作物种质资源中心 | Beans Salt resistant test device and measuring method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101017478A (en) * | 2007-03-09 | 2007-08-15 | 山东省农业科学院土壤肥料研究所 | Computer estimation method for salt tolerance of non-halophyte herbaceous plant |
| CN101341852A (en) * | 2007-08-10 | 2009-01-14 | 江苏省中国科学院植物研究所 | Cross breeding method for black cypress and southern cypress |
| CN103430784A (en) * | 2013-08-23 | 2013-12-11 | 江苏沿江地区农业科学研究所 | Simple method for evaluating salt tolerance of crops at seedling stage |
-
2017
- 2017-05-05 CN CN201710311235.6A patent/CN108781850A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101017478A (en) * | 2007-03-09 | 2007-08-15 | 山东省农业科学院土壤肥料研究所 | Computer estimation method for salt tolerance of non-halophyte herbaceous plant |
| CN101341852A (en) * | 2007-08-10 | 2009-01-14 | 江苏省中国科学院植物研究所 | Cross breeding method for black cypress and southern cypress |
| CN103430784A (en) * | 2013-08-23 | 2013-12-11 | 江苏沿江地区农业科学研究所 | Simple method for evaluating salt tolerance of crops at seedling stage |
Non-Patent Citations (4)
| Title |
|---|
| 环姣姣 等: "盐胁迫对‘中山杉405’及其亲本叶绿体结构的影响", 《林业科技开发》 * |
| 翁跃进: "《作物耐盐品种及其栽培技术》", 31 January 2003, 中国农业出版社 * |
| 陈学森: "《植物育种学实验》", 30 June 2004, 高等教育出版社 * |
| 陈永辉 等: "中山杉无性系耐盐力的水培试验", 《江苏林业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110320208A (en) * | 2019-08-09 | 2019-10-11 | 山东省农作物种质资源中心 | Beans Salt resistant test device and measuring method |
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Application publication date: 20181113 |