CN104782410A - Identification method for maize variety resistance to ralstonia solanacearum - Google Patents
Identification method for maize variety resistance to ralstonia solanacearum Download PDFInfo
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- CN104782410A CN104782410A CN201510130851.2A CN201510130851A CN104782410A CN 104782410 A CN104782410 A CN 104782410A CN 201510130851 A CN201510130851 A CN 201510130851A CN 104782410 A CN104782410 A CN 104782410A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses an identification method for maize variety resistance to ralstonia solanacearum. Self-selected corn variety is selected, the corn is sowed in Sanya, Hainan province in early Feb, the corn is planted with an equidistant row, the planting density is increased by 50% on the basis of a suitable density, the row length is 6 m, the row spacing is 60 cm, 6 rows are existed in each region, the area is of a small region is 21.6 m2, repetition is conducted for 3 times, common field management is adopted, the morbidity of the ralstonia solanacearum of each maize variety in the small region is surveyed one by one during a ripening stage, the surveyed key positions are leaves and 1 to 3 septate internodes of base, the total number of the surveyed corn, the morbidity number of the corn and the disease grade are recorded, and the morbidity = (morbidity number/ total number of the surveyed corn) ) *100%.. The identification method for maize variety resistance to ralstonia solanacearum has the advantages that the morbidity of ralstonia solanacearum of the corn variety can be improved greatly through the arrangement of the identification condition, a more favorable condition is provided for the effective identification of ralstonia solanacearum resistance of the corn variety, identification on ralstonia solanacearum can be conducted one year ahead, and the identification time is shortened.
Description
Technical field
The invention belongs to field of agricultural production technologies, be specifically related to a kind of authentication method of corn variety Resistance to bacterial wilt.
Background technology
Corn Stalk Rot, also known as corn stalk rot disease, Causal Organism of Maize Basal Stalk etc., is a kind of silborne fungal diseases of systemic infection, and having the advantages that morbidity is unexpected, development is rapid, the underproduction is serious, control is difficult, is one of corn producing region, world Major Diseases.Generally believing that the cause of disease of bacterial wilt is complicated, is caused by multiple pathogen Combined Infection, its generation and popularly have substantial connection with kind, growing environment, climatic factor etc.In recent years, Corn Stalk Rot generally occurs in corn seed producing field, China various places and production land for growing field crops, has increase the weight of trend in some areas.Corn Stalk Rot diseased plant rate light below 10%, generally 20% ~ 30%, serious plot up to 80% ~ 90%, to corn safety produce cause serious threat.Selection and popularization disease-resistant variety is the main method of defence bacterial wilt, and it is very important to carry out effective Resistance Identification in the different seed selection stage.
The qualification of current people to corn variety Resistance to bacterial wilt adopts the method for land for growing field crops natural conditions qualification and artificial infection idenfication more.Affect by natural environmental condition, between different regions, different year, bacterial wilt generation and the morbidity order of severity differ greatly, and bring very large difficulty to land for growing field crops natural conditions qualification corn variety Resistance to bacterial wilt.Adopt the method identification result of artificial infection good but to waste time and energy and appraisal cost is higher, and being difficult to carry out Large scale identification.Therefore, no matter be adopt natural environmental condition qualification or adopt Inoculation Method evaluation and screening Resistance to bacterial wilt kind, have significant limitation.So, need a kind of method of simple Rapid identification bacterial wilt disease resistance efficiently on producing at present badly.
Summary of the invention
Technical problem to be solved by this invention is higher for evaluation and screening Corn Stalk Rot resistance cost, and be difficult to carry out the defect of Large scale identification, a kind of method of efficient Rapid identification corn variety bacterial wilt disease resistance is provided, significantly improves the determination rates of corn variety Resistance to bacterial wilt.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
An authentication method for corn variety Resistance to bacterial wilt, qualification condition is as follows:
(1) selection of environment is identified: carry out in Sanya, Hainan early Febuary to early June, climatological data display over nearly 30 years, Hainan monthly mean temperature in May-6 is 28.30 DEG C, medial humidity is 81.68%, total rainfall is 317.98mm, total sunshine duration is 442.01h, this stage is the critical stage of corn grouting-ripe, suddenly clear up after often there is rain weather, and bacterial wilt very easily occurs;
(2) setting of density is identified: increase by 50% on kind Suitable Density basis;
(3) field planting: in early Febuary sowing, wait line-spacing to plant, row long 6m, line-spacing 60cm, every district 6 row, plot area 21.6m
2, repeat for 3 times;
(4) trial: apply fertilizer and to topdress at twice by 600Kg/ per mu yield level, after planting water cover-head water, pour water in time after jointing, keeps ground moistening, and other management are with conventional land for growing field crops;
(5) investigation method: investigate the bacterial wilt incidence of each community corn variety by strain in ripening stage, records the total strain number of investigation, morbidity strain number and disease rank, diseased plant rate=(morbidity strain number/investigate total strain number) × 100%;
An authentication method for corn variety Resistance to bacterial wilt, concrete authentication step is as follows:
(1) sowed in early Febuary at Sanya, Hainan by institute's identification of species, wait line-spacing to plant, planting density increases by 50% on Suitable Density basis, row long 6m, line-spacing 60cm, every district 6 row, plot area 21.6m
2, repeat for 3 times;
(2) fertilising is topdressed at twice by 600Kg/ per mu yield level, other management are with conventional land for growing field crops, investigate the bacterial wilt incidence of each community corn variety in ripening stage by strain, record the total strain number of investigation, morbidity strain number and disease rank, diseased plant rate=(morbidity strain number/investigate total strain number) × 100%.
During described step (2) is topdressed for twice, first time topdresses and imposes composite fertilizer (N:P in seedling stage
2o
5: K
2o=15:15:15) 50 Kg/ mus, second time imposes urea 15-20Kg/ mu in the typhon mouth phase.
Described step (2) investigates the bacterial wilt incidence of each community corn variety in ripening stage by strain, and investigation keypoint part is blade and base portion 1-3 stipes.
Beneficial effect of the present invention: 1. the present invention makes full use of the abundant sunlight-heat reaources of Hainan in Winter, and the natural conditions that temperature is high, high humidity easily sends out Corn Stalk Rot evil, just Resistance to bacterial wilt qualification can be carried out under field conditions (factors), solve and need to arrange the restriction of hot and humid condition when northern China carries out Resistance to bacterial wilt qualification, and the problem of time and effort consuming during artificial infection inducing maize bacterial wilt disease.Greatly reduce appraisal cost.2. what the present invention identified condition arranges the diseased plant rate that greatly can improve corn variety bacterial wilt, for effective qualification of corn variety Resistance to bacterial wilt creates more favourable condition.3. adopt authentication method of the present invention, can carry and the previous year bacterial wilt being identified, shorten qualification time.
Embodiment
Embodiment 1
Select self-fertile corn variety Henan list 602, Henan list 604, Henan list 605, Henan list 606, Henan single 607 and Henan list 608, sow in early Febuary at Sanya, Hainan, wait line-spacing to plant, planting density is 4000 plants/acre, row long 6m, line-spacing 60cm, every district 6 row, plot area 21.6m
2, repeat for 3 times, apply fertilizer and to topdress at twice by 600Kg/ per mu yield level, first time topdresses and imposes composite fertilizer 50 Kg/ mu (N:P in seedling stage
2o
5: K
2o=15:15:15), second time chases after urea 15 ~ 20Kg at typhon mouth phase mu, after planting water cover-head water, seedling stage, jointing, weave silk, the pustulation period respectively waters once, other management, with general land for growing field crops, investigate the bacterial wilt incidence of each community corn variety in ripening stage by strain, investigation blade and base portion 1-3 stipes, record the total strain number of investigation, morbidity strain number and disease rank, diseased plant rate=(morbidity strain number/investigate total strain number) × 100%.
Embodiment 2
Planting density is 5000 plants/acre, and all the other steps are with embodiment 1.
Embodiment 3
Planting density is 6000 plants/acre, and all the other steps are with embodiment 1.
Table 1 be Sanya, Hainan February-climatological data in 30 years June, as shown in Table 1, climatological data display over nearly 30 years, Hainan monthly mean temperature in May-6 is 28.30 DEG C, medial humidity is 81.68%, total rainfall is 317.98mm, total sunshine duration is 442.01h, this stage is the critical stage of corn grouting-ripe, suddenly clear up after often there is rain weather, and bacterial wilt very easily occurs.Hainan early Febuary-the experimental enviroment condition of early June is beneficial to the generation of bacterial wilt, the present invention identifies that the effective qualification of the selection of environment to kind Resistance to bacterial wilt provides good natural conditions.
Table 1 Hainan duration of test 30 year-climate data (1984-2014)
The bacterial wilt diseased plant rate of corn variety under different densities condition is as shown in table 2.
Table 2 corn variety is bacterial wilt diseased plant rate (%) under 3 planting densities
As shown in Table 2, along with the increase of planting density, the bacterial wilt diseased plant rate of each kind is in increasing the weight of trend gradually.The bacterial wilt diseased plant rate of each kind comparatively improves 142.80% under density conditions of the present invention under Suitable Density condition.As can be seen here, qualification density of the present invention the bacterial wilt diseased plant rate that greatly can improve corn variety is set, for effective qualification of corn variety Resistance to bacterial wilt creates more favourable condition.
Claims (4)
1. an authentication method for corn variety Resistance to bacterial wilt, is characterized in that qualification condition is as follows:
(1) selection of environment is identified: carry out in Sanya, Hainan early Febuary to early June;
(2) setting of density is identified: increase by 50% on kind Suitable Density basis;
(3) field planting: in early Febuary sowing, wait line-spacing to plant, row long 6m, line-spacing 60cm, every district 6 row, repeats for 3 times;
(4) trial: apply fertilizer and to topdress at twice by 600Kg/ per mu yield level, other management are with conventional land for growing field crops;
(5) investigation method: investigate the bacterial wilt incidence of each community corn variety by strain in ripening stage, records the total strain number of investigation, morbidity strain number and disease rank, diseased plant rate=(morbidity strain number/investigate total strain number) × 100%.
2. the authentication method of corn variety Resistance to bacterial wilt according to claim 1, is characterized in that concrete authentication step is as follows:
(1) sowed in early Febuary at Sanya, Hainan by institute's identification of species, wait line-spacing to plant, planting density increases by 50% on Suitable Density basis, row long 6m, line-spacing 60cm, every district 6 row, plot area 21.6m
2, repeat for 3 times;
(2) fertilising is topdressed at twice by 600Kg/ per mu yield level, other management are with conventional land for growing field crops, investigate the bacterial wilt incidence of each community corn variety in ripening stage by strain, record the total strain number of investigation, morbidity strain number and disease rank, diseased plant rate=(morbidity strain number/investigate total strain number) × 100%.
3. the authentication method of corn variety Resistance to bacterial wilt according to claim 2, in it is characterized in that described step (2) is topdressed for twice, first time topdresses and imposes composite fertilizer 50 Kg/ mu in seedling stage, and second time imposes urea 15-20Kg/ mu in the typhon mouth phase.
4. the authentication method of corn variety Resistance to bacterial wilt according to claim 2, is characterized in that described step (2) investigates the bacterial wilt incidence of each community corn variety in ripening stage by strain, and investigation keypoint part is blade and base portion 1-3 stipes.
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| CN201510130851.2A CN104782410B (en) | 2015-03-25 | 2015-03-25 | A kind of authentication method of corn variety Resistance to bacterial wilt |
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| CN104782410B CN104782410B (en) | 2017-10-27 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980499A (en) * | 2017-12-25 | 2018-05-04 | 河北省农林科学院粮油作物研究所 | A kind of method of sesame droop disease resistance field test |
| CN109717029A (en) * | 2019-03-12 | 2019-05-07 | 河南农业大学 | A method of Corn Stalk Rot resistance is improved by companion cropping |
| CN111727878A (en) * | 2020-07-27 | 2020-10-02 | 湖北康农种业股份有限公司 | Screening method of maize bacterial wilt resistant plants |
| CN113693033A (en) * | 2021-09-14 | 2021-11-26 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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| CN86105267A (en) * | 1986-08-06 | 1988-07-06 | 云南省农科院粮食作物研究所 | Be exclusively used in the method for native maize varieties improvement |
| CN1100256A (en) * | 1994-03-18 | 1995-03-22 | 沈阳农业大学 | Planting technique for increasing production of maize |
| CN1600076A (en) * | 2003-09-27 | 2005-03-30 | 陈学君 | Method for breeding hybrid corn in use as for both foodstuff and feedstuff |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980499A (en) * | 2017-12-25 | 2018-05-04 | 河北省农林科学院粮油作物研究所 | A kind of method of sesame droop disease resistance field test |
| CN109717029A (en) * | 2019-03-12 | 2019-05-07 | 河南农业大学 | A method of Corn Stalk Rot resistance is improved by companion cropping |
| CN111727878A (en) * | 2020-07-27 | 2020-10-02 | 湖北康农种业股份有限公司 | Screening method of maize bacterial wilt resistant plants |
| CN113693033A (en) * | 2021-09-14 | 2021-11-26 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
| CN113693033B (en) * | 2021-09-14 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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Inventor after: Li Chaohai Inventor after: Li Hongping Inventor after: Huang Lianfu Inventor after: Dong Pengfei Inventor after: Yang Chunshou Inventor after: Jiang Hantao Inventor after: Zhang Haihong Inventor before: Li Chaohai Inventor before: Huang Lianfu Inventor before: Li Hongping Inventor before: Dong Pengfei Inventor before: Yang Chunshou Inventor before: Jiang Hantao Inventor before: Zhang Haihong |
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