CN106472091A - Corn seed purity field plot field plot test method - Google Patents

Corn seed purity field plot field plot test method Download PDF

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Publication number
CN106472091A
CN106472091A CN201610906502.XA CN201610906502A CN106472091A CN 106472091 A CN106472091 A CN 106472091A CN 201610906502 A CN201610906502 A CN 201610906502A CN 106472091 A CN106472091 A CN 106472091A
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sample
color
field plot
plant
row
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CN201610906502.XA
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Inventor
孟小莽
王灵敏
袁瑞江
赵海龙
柴玉坡
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Shijiazhuang Seed Management Station
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Shijiazhuang Seed Management Station
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Priority to CN201610906502.XA priority Critical patent/CN106472091A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention relates to corn seed authentication method, especially corn seed purity field plot field plot test method, comprise the following steps:(1) to for the registration of sample product, sequence;(2) obtain standard sample and collect the description of variety characteristic;(3) determine the grain number of plantation sample;(4) number takes plantation sample and numbers;(5) select plot;Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-narrow row planting according to rainfall distributed areas respectively, described Ridged plant adopts in area of heavy rainfull, and described flat furrow wide-narrow row planting adopts in area short of rain;(6) sow;(7) seedling stage assay;(8) florescence identification;(9) period of maturation identification.The method, in the periods such as Maize Seedlings, florescence, period of maturation, the difference of plant forms feature and biological characteristicses, identifies special-shaped plant, thus identifying seed purity it is ensured that seed quality is it is ensured that safety is planted in agricultural production.

Description

Corn seed purity field plot field plot test method
Technical field
The present invention relates to corn seed authentication method, especially corn seed purity field plot field plot test method.
Background technology
Improved seeds productive potentialities are largely dependent upon seed quality, and the verity of seed and purity are to evaluate to plant The good and bad important indicator of protonatomic mass, the lower general who has surrendered of purity leads to the obvious reduction of seed production and quality.Field trapping test method As the most accurate, Purity method the most authoritative, therefore there is seed purity in people one straight grip field plot test for a long time During dispute, typically all arbitration inspection is carried out with field plot test.During field plot test, the selection to plantation soil is more single at present One, generally in June early and middle ten dayses, now milpa growth is in especially florescence megathermal period on the occasion of high temperature rain to seedtime In season, it is unfavorable for the abundant performance of corn variety feature;Thickness of sowing is generally lower than normal plantation, for identification kind Plant, be unfavorable for the growth of Semen Maydiss, affect qualification result.Generally adopt florescence identification, identification number of times is less, and result is had an impact.
Content of the invention
For solving the above problems, the present invention provides a kind of single cave sowing, the sowing of different soils, one kind of multiple contrast record Corn seed purity field plot field plot test method, concrete technical scheme is:
Corn seed purity field plot field plot test method, comprises the following steps:
(1) to for the registration of sample product, sequence;
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this row Sequence number writes specimen coding;
(2) obtain standard sample and collect the description of variety characteristic;
The standard sample of parent and cenospecies is collected at breeder and its agent or variety managements department, and collection Plant feature related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch How much, anther color, fringe shape, cob color, particle shape, grain color;
(3) determine the grain number of plantation sample;
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine examination Test the plantation strain number of design, if standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, is Ensure that plantation strain number meets 4N principle, plantation sample grain number should be 4N/ germination percentage;
(4) number takes plantation sample and numbers;
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, according to confession Specimen coding is write in the sequence number of sample product, is easy to the sample adjacent seed same kind;
(5) select plot;
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies Or Parent does comparison and is compared, to judge its typical case, consistent degree, often repeat to plant two row, using north and south row to plantation;
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-and narrow-row kind according to rainfall distributed areas respectively Plant, described Ridged plant adopts in area of heavy rainfull, described flat furrow wide-narrow row planting adopts in area short of rain;
(6) sow;
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses mark Labelling is as the site of program request;Use plastic ties labelling, plastic ties press fixing spacing in the rows amount good position, uses different colours line Rope binding marking, as the site of program request, with bamboo stem and plastic ties as scale, makes spacing in the rows uniformity, and plant erects Straight it is ensured that the growing space of the equalization of every plant;
(7) seedling stage assay;With seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and length Gesture is identified, and fills in corn seed purity field plot field plot test application form;
(8) florescence identification;Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape And branch loose compactness, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position how many with branch, Panel length is identified, and fills in corn seed purity field plot field plot test application form;
(9) period of maturation identification;Entered with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color Row identification, and fill in corn seed purity field plot field plot test application form.
Described Ridged plant, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm;Using duplicate rows kind on ridge Plant, the line-spacing of its duplicate rows is 100cm, and as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample size and plant Away from determining cell length and width, cell surrounding sets 4-5 row protection row;Described flat furrow wide-narrow row planting, wide row line-spacing 80- 90cm, as observation road;Narrow row 30-40cm;Spacing in the rows 30-40cm.
Described sowing time was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and planted the sowing time than maize field Postpone 20 days.
It is chosen at area of heavy rainfull Ridged plant according to rainfall distributed areas respectively and in regional flat furrow wide-and narrow-row kind short of rain Plant, reduce the impact to cultivar identification for the rainfall, that is, reduce the impact to Purity Identification for the extraneous factor.
Using simple grain bunch planting, and mark in detail, be easy to record and manage, ensure the growth of the equalization of every plant simultaneously Space, reduces the error that external factor leads to.
Normal field corn this area June sowing time early and middle ten dayses, it is especially colored that milpa growth is in the megathermal period Phase, on the occasion of high temperature rainy season, is unfavorable for the abundant performance of corn variety feature, Postpone sowing time, can improve its growth ring Border, beneficial to florescence identification.
Increase the identification in seedling stage and period of maturation.Corn variety quantity is very many at present, and between kind on good multiple characters Have points of resemblance, increase the identification in seedling stage and period of maturation, using three periods feature synthetic determination it is ensured that qualification result Accurate.
Random number takes test specimen.For make limited seed have preferably representative it is necessary to random by regulation seed amount Perfect number takes seed to be sowed.Because if the seed grain number that number takes is more than the Seeding Quantity of regulation, during program request Can choose whichever is to one personal advantage, be left the seed of slight of stature, cause personal error.
The corn seed purity field plot field plot test method that the present invention provides is in Maize Seedlings, florescence, period of maturation , according to the difference of plant forms feature and biological characteristicses, different in nature plant is identified in period, thus identifying seed purity, Guarantee seed quality it is ensured that safety is planted in agricultural production.
Specific embodiment
Corn seed purity field plot field plot test method, comprises the following steps:
(1) to for the registration of sample product, sequence;
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this row Sequence number writes specimen coding.
(2) obtain standard sample and collect the description of variety characteristic;
The standard sample of parent and cenospecies is collected at breeder and its agent or variety managements department, and collection Plant feature related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch How much, anther color, fringe shape, cob color, particle shape, grain color.
(3) determine the grain number of plantation sample;
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine examination Test the plantation strain number of design.If standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, is Ensure that plantation strain number meets 4N principle, plantation sample grain number should be 4N/ germination percentage.
(4) number takes plantation sample and numbers;
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, according to confession Specimen coding is write in the sequence number of sample product, is easy to the sample adjacent seed same kind.
(5) select plot;
Select the suitable plant identification traitses normal development of climatic environment condition, soil fertility uniformly, fertile soil, have irrigation and drainage bar Part, front stubble are not Semen Maydiss and the plot of no disease and pests harm and weeds.
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies Or Parent does comparison and is compared, to judge its typical case, consistent degree, often repeat to plant two row, using north and south row to plantation;
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-and narrow-row kind according to rainfall distributed areas respectively Plant, described Ridged plant adopts in area of heavy rainfull, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm;Using ridge Upper duplicate rows plantation, the line-spacing of its duplicate rows is 100cm, and as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample number Amount and seeding row spacing determine cell length and width, and cell surrounding sets 4-5 row protection row;
Described flat furrow wide-narrow row planting adopts in area short of rain, wide row line-spacing 80-90cm, as observation road, narrow row 30- 40cm;Spacing in the rows 30-40cm.
(6) sow;
Pre-sowing water should be poured before broadcasting, fine site preparation, use sufficient base fertilizer, N, P, K should join in proportion and apply.
Seed should be carried out before broadcasting with dry in the sun, promote germination.For preventing insect pest and the plague of rats, should carry out to being uncoated seed Dressing seed etc. is processed.
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses mark Labelling is as the site of program request;Use plastic ties labelling, plastic ties press fixing spacing in the rows amount good position, uses different colours line Rope binding marking, as the site of program request, with bamboo stem and plastic ties as scale, makes spacing in the rows uniformity, and plant erects Directly it is ensured that the growing space of the equalization of every plant, depth of planting 3-5cm depth of planting, thickness of earth covering and pressing dynamics will be use up Amount is consistent.
Sowing time was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and postponed than the maize field plantation sowing time 20 days.
The time of infertility at least intertills twice, especially after raining, loosening the soil in time it is ensured that having gone out Seedling, must not fill the gaps with seedlings. Carry out in seedling stage 5-6 leaf for the first time, tilling depth 3-4cm, second carries out deep cultivation and banking inferior to after jointing before envelope ridge.Also can be Spray herbicide after finishing.
Topdress once in the jointing stage, watering for advocating peace to combine with quick-acting N fertilizer is carried out.To pour permeable before taking out hero, prevention Card neck drought, pours a water to increase grain plumpness before grouting.
The harm of the disease such as insect pest and smut, virosiss such as preventing and treating subterranean pest-insect, mythimna separata, Pyrausta nubilalis (Hubern). and aphid in time.
(7) seedling stage assay;With seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and length Gesture is identified, and fills in corn seed purity field plot field plot test application form, such as table 1;
(8) florescence identification;Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape And branch loose compactness, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position how many with branch, Panel length is identified, and fills in corn seed purity field plot field plot test application form;
(9) period of maturation identification;Entered with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color Row identification, and fill in corn seed purity field plot field plot test application form.
Identification must be completed jointly by more than two accredited Seed Inspection persons.With father, female parent and the cenospecies mark collected The phene of quasi- sample is as standard.Fill in identification strictly according to the facts to record, strain label or plastics board are made to be suspected to seedling stage, florescence Labelling.Synthetic determination again after the identification of each period of duration, still thinks that the plant being like right and wrong should be by this breed count.
Computing formula:
Use《GB/T3543.5》The Deviation of Eggs Per Gram of table 2 regulation, if can not find out in table, available following formula is calculated:
P variety mark value;
Q=100-P;
N kind plants strain number.
Qualification result with seedling stage as reference, with tree characteristics performance the most completely, based on the most obvious florescence and fruit ear phase, with The meansigma methodss repeating twice represent, retain a decimal.Figure correction executes《GB8170-87 Figure correction rule》.Various one-tenth The percentage rate summation divided is necessary for 100.0%.
Table 1 corn seed purity field plot field plot test application form
Explanation:Write the color of anthocyanin when the 1st, describing exactly, and indicate weak, medium, strong;
2nd, in addition to the content of this table regulation, other contents with kind typical case's character all can be supplemented.

Claims (3)

1. field plot test method in corn seed purity field plot is it is characterised in that comprise the following steps:
(1) to for the registration of sample product, sequence;
According to variety name, source, working year, seed quality index, registered and by kind sequence, according to this sequence number Write specimen coding;
(2) obtain standard sample and collect the description of variety characteristic;
Collect the standard sample of parent and cenospecies at breeder and its agent or variety managements department, and it is special to collect kind Intrinsic characteristics related data, including sheath color, leaf margin color, leaf, plant shape, plant height, Ear height, filigree color, tassel and branch how many, Anther color, fringe shape, cob color, particle shape, grain color;
(3) determine the grain number of plantation sample;
According to state quality standard《GB 4404.1-2008》The Reinheitszahl of regulation or label for labelling Reinheitszahl, to determine that test sets The plantation strain number of meter, if standard is (N-1) × 100%/N, plantation strain number is 4N, because each sample germination percentage differs, for ensureing Plantation strain number meets 4N principle, and plantation sample grain number should be 4N/ germination percentage;
(4) number takes plantation sample and numbers;
Plant the grain number of sample according to step (3), random number takes two parts of field plot test sample from for sample product, foundation supplies sample Specimen coding is write in the sequence number of product, is easy to the sample adjacent seed same kind;
(5) select plot;
Each sample sets 2 repetitions, and the sample adjacent seed of same kind, then adjacent seed standard sample is cenospecies or father Female parent does comparison and is compared, and to judge its typical case, consistent degree, often repeats to plant two row, using north and south row to plantation;
Choose two kinds of cultivation modes, respectively Ridged plant peace furrow wide-narrow row planting, institute according to rainfall distributed areas respectively State Ridged plant to adopt in area of heavy rainfull, described flat furrow wide-narrow row planting adopts in area short of rain;
(6) sow;
Adopt simple grain bunch planting in sowing time, use bamboo stem labelling, long bamboo stem presses fixing spacing in the rows amount good position, uses marking pen mark It is denoted as the site for program request;Use plastic ties labelling, fixing spacing in the rows amount good position is pressed on plastic ties, with different colours cotton rope bundle Tie up marking, as the site of program request, with bamboo stem and plastic ties as scale, make spacing in the rows uniformity, and plant is vertically, protects The growing space of the equalization of every plant of card;
(7) seedling stage assay;Entered with seedling sheath color, leaf, leaf color, leaf edge color, blade ripple and degreeof tortuosity and growing way Row identification, and fill in corn seed purity field plot field plot test application form;
(8) florescence identification;Had or not with fine hair on plant type, leaf, leaf color, leaf, vein color, auricle shape, tassel shape and point Branch is how many and the loose compactness of branch, sterile lemma color, flower pesticide color, filigree color, blade opening angle, the number of blade and fringe position, internode Length is identified, and fills in corn seed purity field plot field plot test application form;
(9) period of maturation identification;Reflected with reference to resistance, period of duration with fringe type, particle shape, grain color, seed size and cob color Fixed, and fill in corn seed purity field plot field plot test application form.
2. corn seed purity field plot according to claim 1 field plot test method is it is characterised in that described ridging Plantation, row spacing 100-130cm, furrow width 30cm about, ditch depth 20-30cm;Planted using duplicate rows on ridge, the line-spacing of its duplicate rows is 100cm, as observation road, Planting Row Distance ridge side 10-15cm on ridge, according to sample size and seeding row spacing determine cell length and Width, cell surrounding sets 4-5 row protection row;Described flat furrow wide-narrow row planting, wide row line-spacing 80-90cm, as observation road;Narrow Row 30-40cm;Spacing in the rows 30-40cm.
3. corn seed purity field plot according to claim 1 field plot test method is it is characterised in that described sowing Phase was broadcast for evening, and 30-July 10 Ji ALFISOL IN CENTRAL June is sowed, and postponed 20 days than the maize field plantation sowing time.
CN201610906502.XA 2016-10-18 2016-10-18 Corn seed purity field plot field plot test method Pending CN106472091A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108650946A (en) * 2018-05-08 2018-10-16 兰州大学 A kind of uniform type of seeding of grid location based on crop ecology experiment
CN110651626A (en) * 2018-09-10 2020-01-07 湖南省林产品质量检验检测中心 Identification method of masson pine and black pine seeds
CN111837865A (en) * 2020-06-28 2020-10-30 中国农业科学院棉花研究所 Method for identifying purity of cotton variety and operation steps thereof
CN112931099A (en) * 2021-03-23 2021-06-11 石家庄市种子管理站 Method for identifying purity of winter wheat seeds by planting in field plot

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108650946A (en) * 2018-05-08 2018-10-16 兰州大学 A kind of uniform type of seeding of grid location based on crop ecology experiment
CN110651626A (en) * 2018-09-10 2020-01-07 湖南省林产品质量检验检测中心 Identification method of masson pine and black pine seeds
CN111837865A (en) * 2020-06-28 2020-10-30 中国农业科学院棉花研究所 Method for identifying purity of cotton variety and operation steps thereof
CN112931099A (en) * 2021-03-23 2021-06-11 石家庄市种子管理站 Method for identifying purity of winter wheat seeds by planting in field plot

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Application publication date: 20170308