CN104782410B - A kind of authentication method of corn variety Resistance to bacterial wilt - Google Patents
A kind of authentication method of corn variety Resistance to bacterial wilt Download PDFInfo
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- CN104782410B CN104782410B CN201510130851.2A CN201510130851A CN104782410B CN 104782410 B CN104782410 B CN 104782410B CN 201510130851 A CN201510130851 A CN 201510130851A CN 104782410 B CN104782410 B CN 104782410B
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Abstract
The invention discloses a kind of authentication method of corn variety Resistance to bacterial wilt, the free kind of corn is selected, is sowed in Sanya, Hainan in early Febuary, Deng line-spacing plantation, planting density increases by 50%, the long 6m of row on the basis of Suitable Density, line-spacing 60cm, per the row of area 6, plot area 21.6m2, 3 repetitions, using conventional field management, in bacterial wilt incidence of the dough stage by each cell corn variety of strain investigation, investigation keypoint part is blade and the stipes of base portion 13, records and investigates total strain number, strain number of falling ill and disease rank, diseased plant rate=（Morbidity strain number/investigation total strain number）× 100%, the setting of identification condition of the present invention can greatly improve the diseased plant rate of corn variety bacterial wilt, be that effective identification of corn variety Resistance to bacterial wilt creates more favourable condition, using the authentication method of the present invention, can carry the previous year identifies bacterial wilt, shortens qualification time.
Description
Technical field
The invention belongs to field of agricultural production technologies, and in particular to a kind of authentication method of corn variety Resistance to bacterial wilt.
Background technology
Corn Stalk Rot is also known as corn stalk rot disease, Causal Organism of Maize Basal Stalk etc., is a kind of silborne fungal diseases of systemic infection,
With morbidity suddenly, quickly grow, the characteristics of the underproduction is serious, preventing and treating is difficult, be one of world corn producing region Major Diseases.Generally
Think that the cause of disease of bacterial wilt is complicated, be its generation and popular and kind, growth ring caused by a variety of pathogen Combined Infections
Border, climatic factor etc. have substantial connection.In recent years, Corn Stalk Rot is generally sent out in China various regions corn seed producing field and production crop field
It is raw, there is exacerbation trend in some areas.Corn Stalk Rot diseased plant rate it is light below 10%, typically 20% ~ 30%, seriously
Plot is up to 80% ~ 90%, causes serious threat to corn safety production.Seed selection and popularization disease-resistant variety are defence bacterial wilts
Main method, and it is particularly significant to carry out effective Resistance Identification in the different seed selection stages.
Current people using the identification of crop field natural conditions and artificial infection to being reflected more than the identification of corn variety Resistance to bacterial wilt
Fixed method.Influenceed by natural environmental condition, bacterial wilt occurs between different regions, different year and the morbidity order of severity is differed very
Greatly, very big difficulty is brought to crop field natural conditions identification corn variety Resistance to bacterial wilt.Effect is identified using the method for artificial infection
Fruit is good but wastes time and energy and appraisal cost is higher, and is difficult to carry out Large scale identification.Therefore, either using natural environment bar
Part identification still uses Inoculation Method evaluation and screening Resistance to bacterial wilt kind, there is significant limitation.So, it is raw at present
Need a kind of method of simple efficient Rapid identification bacterial wilt disease resistance in production badly.
The content of the invention
The technical problems to be solved by the invention are higher for evaluation and screening Corn Stalk Rot resistance cost, and are difficult to
Implement the defect of Large scale identification there is provided a kind of method of efficient Rapid identification corn variety bacterial wilt disease resistance, significantly improve
The determination rates of corn variety Resistance to bacterial wilt.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of authentication method of corn variety Resistance to bacterial wilt, identification condition is as follows：
（1）Identify the selection of environment：Carried out in Sanya, Hainan early Febuary to early June, climatological data shows over nearly 30 years
Show, Hainan May -6 monthly mean temperature be 28.30 DEG C, medial humidity is when to be 81.68%, total rainfall be 317.98mm, total sunshine
Number is 442.01h, and this stage is the critical stage of corn grouting-maturation, cleared up suddenly after rain weather, bacterial wilt pole often occurs
Easily occur；
（2）Identify the setting of density：Increase by 50% on the basis of kind Suitable Density；
（3）Field planting：In early Febuary sowing, line-spacing is waited to plant, row long 6m, line-spacing 60cm, per the row of area 6, plot area
21.6m2, 3 repetitions；
（4）Trial：Fertilising is topdressed in two times by 600Kg/ per mu yields level, after planting pour after cover-head water, jointing and
When pour water, keep ground moistening, other management are with conventional crop field；
（5）Investigation method：The bacterial wilt incidence of each cell corn variety is investigated by strain in dough stage, investigation is recorded total
Strain number, morbidity strain number and disease rank, diseased plant rate=（Morbidity strain number/investigation total strain number）×100%；
A kind of authentication method of corn variety Resistance to bacterial wilt, specific authentication step is as follows：
（1）Institute's identification of species is sowed in Sanya, Hainan in early Febuary, waits line-spacing to plant, planting density is in Suitable Density
On the basis of increase by 50%, row long 6m, line-spacing 60cm, per the row of area 6, plot area 21.6m2, 3 repetitions；
（2）Fertilising is topdressed in two times by 600Kg/ per mu yields level, and other management are adjusted with conventional crop field in dough stage by strain
The bacterial wilt incidence of each cell corn variety is looked into, records and investigates total strain number, morbidity strain number and disease rank, diseased plant rate=
（Morbidity strain number/investigation total strain number）×100%.
The step（2）In topdressing twice, topdress impose composite fertilizer in seedling stage for the first time（N:P2O5:K2O=15:15:15）
50 Kg/ mus, impose 15-20Kg/ mus of urea in the typhon mouthful phase for the second time.
The step（2）The bacterial wilt incidence of each cell corn variety, investigation emphasis portion are investigated by strain in dough stage
Position is blade and base portion 1-3 stipes.
Beneficial effects of the present invention：1. the present invention makes full use of the abundant sunlight-heat reaources of Hainan in Winter, and temperature is high, wet
The natural conditions of great Yi hair Corn Stalk Rot evils are spent, Resistance to bacterial wilt identification is can be carried out under field conditions (factors), is solved
Northern China carries out needing to set the limitation of hot and humid condition, and artificial infection inducing maize green grass or young crops during Resistance to bacterial wilt identification
During rot disease the problem of time and effort consuming.Greatly reduce appraisal cost.2. the setting of identification condition of the present invention can be carried significantly
The diseased plant rate of high corn variety bacterial wilt, is that effective identification of corn variety Resistance to bacterial wilt creates more favourable condition.
3. using the authentication method of the present invention, can carry the previous year identifies bacterial wilt, shortens qualification time.
Embodiment
Embodiment 1
Self-fertile corn variety Henan single 602, Henan single 604, Henan single 605, Henan single 606, Henan single 607 and Henan single 608 are selected, in sea
South three is second to early Febuary sowing, waits line-spacing to plant, and planting density is 4000 plants/acre, row long 6m, line-spacing 60cm, and every area 6 row is small
Area area 21.6m2, 3 repetitions, fertilising topdresses in two times by 600Kg/ per mu yields level, and topdressing for the first time, it is multiple to be imposed in seedling stage
Kg/ mus of Hefei 50（N:P2O5:K2O=15:15:15）, 15 ~ 20Kg of urea is chased after in typhon mouthful phase mu for the second time, illiteracy is after planting poured
Head water, seedling stage, jointing, spinning, pustulation period respectively water once, and other management investigate each small in dough stage with general crop field by strain
The bacterial wilt incidence of area's corn variety, investigation blade and base portion 1-3 stipes, record investigation total strain number, morbidity strain number and disease
Evil rank, diseased plant rate=（Morbidity strain number/investigation total strain number）×100%.
Embodiment 2
Planting density is 5000 plants/acre, remaining step be the same as Example 1.
Embodiment 3
Planting density is 6000 plants/acre, remaining step be the same as Example 1.
Table 1 is the climatological data in 30 years 2 months-June of Sanya, Hainan, and as shown in Table 1, climatological data is shown over nearly 30 years,
Hainan May -6 monthly mean temperature be 28.30 DEG C, medial humidity be that 81.68%, total rainfall is that 317.98mm, total sunshine time are
442.01h, this stage is the critical stage of corn grouting-maturation, the weather that cleared up suddenly after rain often occurs, and bacterial wilt is easily sent out
It is raw.The experimental enviroment condition of Hainan early Febuary-early June is beneficial to the generation of bacterial wilt, and the selection of present invention identification environment is to product
The effective identification for planting Resistance to bacterial wilt provides good natural conditions.
30 year-climate data during the Hainan of table 1 is tested（1984-2014）
Bacterial wilt diseased plant rate of the corn variety under the conditions of different densities is as shown in table 2.
The corn variety of table 2 bacterial wilt diseased plant rate under 3 planting densities（%）
As shown in Table 2, with the increase of planting density, the bacterial wilt diseased plant rate of each kind is in gradually to aggravate trend.Respectively
The bacterial wilt diseased plant rate of kind is under density conditions of the present invention compared with improving 142.80% under the conditions of Suitable Density.
As can be seen here, the setting of identification density of the invention can greatly improve the bacterial wilt diseased plant rate of corn variety, be corn product
The effective identification for planting Resistance to bacterial wilt creates more favourable condition.
Claims (1)
1. a kind of authentication method of corn variety Resistance to bacterial wilt, it is characterised in that identification condition is as follows：
A identifies the selection of environment：Carried out in Sanya, Hainan the first tenday period of a month in 2 month the first tenday period of a month to June；
B identifies the setting of density：Increase by 50% on the basis of kind Suitable Density；
C field plantings：In the sowing of 2 month the first tenday period of a month, line-spacing is waited to plant, row long 6m, line-spacing 60cm, per the row of area 6,3 repetitions；
D trials：Fertilising is topdressed in two times by 600Kg/ per mu yields level, and other management are with conventional crop field；
E investigation methods：The bacterial wilt incidence of each cell corn variety is investigated by strain in dough stage, record investigation total strain number,
Strain number of falling ill and disease rank, diseased plant rate=（Morbidity strain number/investigation total strain number）×100%；
The authentication method of described corn variety Resistance to bacterial wilt, specific authentication step is as follows：
（1）Institute's identification of species was sowed in Sanya, Hainan in 2 month the first tenday period of a month, waits line-spacing to plant, planting density is on Suitable Density basis
Upper increase by 50%, row long 6m, line-spacing 60cm, per the row of area 6, plot area 21.6m2, 3 repetitions；
（2）Fertilising is topdressed in two times by 600Kg/ per mu yields level, and other management are investigated with conventional crop field in dough stage by strain
The bacterial wilt incidence of each cell corn variety, records and investigates total strain number, morbidity strain number and disease rank, and diseased plant rate=（Hair
Diseased plant number/investigation total strain number）×100%；
The step（2）In topdressing twice, topdress Kg/ mus of composite fertilizer 50 is imposed in seedling stage for the first time, second in typhon
The mouth phase imposes 15-20Kg/ mus of urea；
The step（2）The bacterial wilt incidence of each cell corn variety is investigated by strain in dough stage, investigation keypoint part is
Blade and base portion 1-3 stipes.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980499A (en) * | 2017-12-25 | 2018-05-04 | 河北省农林科学院粮油作物研究所 | A kind of method of sesame droop disease resistance field test |
| CN109717029A (en) * | 2019-03-12 | 2019-05-07 | 河南农业大学 | A method of Corn Stalk Rot resistance is improved by companion cropping |
| CN111727878A (en) * | 2020-07-27 | 2020-10-02 | 湖北康农种业股份有限公司 | Screening method of maize bacterial wilt resistant plants |
| CN113693033B (en) * | 2021-09-14 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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| CN86105267A (en) * | 1986-08-06 | 1988-07-06 | 云南省农科院粮食作物研究所 | Be exclusively used in the method for native maize varieties improvement |
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| CN1600076A (en) * | 2003-09-27 | 2005-03-30 | 陈学君 | Method for breeding hybrid corn in use as for both foodstuff and feedstuff |
| CN101889545A (en) * | 2010-08-02 | 2010-11-24 | 辽宁联达种业有限责任公司 | Selective breeding method of high density and high yield corn hybrid |
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2015
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|---|---|---|---|---|
| CN86105267A (en) * | 1986-08-06 | 1988-07-06 | 云南省农科院粮食作物研究所 | Be exclusively used in the method for native maize varieties improvement |
| CN1100256A (en) * | 1994-03-18 | 1995-03-22 | 沈阳农业大学 | Planting technique for increasing production of maize |
| CN1600076A (en) * | 2003-09-27 | 2005-03-30 | 陈学君 | Method for breeding hybrid corn in use as for both foodstuff and feedstuff |
| CN101889545A (en) * | 2010-08-02 | 2010-11-24 | 辽宁联达种业有限责任公司 | Selective breeding method of high density and high yield corn hybrid |
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| 农业部办公厅关于印发《小麦、玉米、水稻三大粮食作物区域大配方与施肥建议(2013)》的通知;农业部;《中华人民共和国农业部公报》;20130802(第8期);第46-50页 * |
| 河南省玉米病害的发生特点和主推品种的田间抗性鉴定;段鹏飞等;《玉米科学》;20100415;第18卷(第2期);第117-120、124页 * |
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Inventor after: Li Chaohai Inventor after: Li Hongping Inventor after: Huang Lianfu Inventor after: Dong Pengfei Inventor after: Yang Chunshou Inventor after: Jiang Hantao Inventor after: Zhang Haihong Inventor before: Li Chaohai Inventor before: Huang Lianfu Inventor before: Li Hongping Inventor before: Dong Pengfei Inventor before: Yang Chunshou Inventor before: Jiang Hantao Inventor before: Zhang Haihong |
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