CN108781950A - A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization - Google Patents

A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization Download PDF

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Publication number
CN108781950A
CN108781950A CN201710311157.XA CN201710311157A CN108781950A CN 108781950 A CN108781950 A CN 108781950A CN 201710311157 A CN201710311157 A CN 201710311157A CN 108781950 A CN108781950 A CN 108781950A
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China
Prior art keywords
hybridization
different
saline
china fir
planting
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CN201710311157.XA
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Chinese (zh)
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杨义茹
王明华
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Jiangsu Traceability Agricultural Science And Technology Development Co Ltd
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Jiangsu Traceability Agricultural Science And Technology Development Co Ltd
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Priority to CN201710311157.XA priority Critical patent/CN108781950A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of methods carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, include the following steps:Hybrid identification;Seedling determination;Salt-Tolerance Identification is carried out to selected hybrid plant;Cutting propagation and Experiment of Container nursing are carried out to hybrid plant;Regional testing;Saline-alkali soil forestation technical standard is formulated, carries out afforestation experiment using different size nursery stock, different planting densities and different types of nursery stock, the above method is for ensureing that the black China fir of hybridization in the survival rate of planting site, ensures the effect of popularizing planting.

Description

A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization
Technical field
It is the present invention relates to hybridization black China fir planting technology field, more particularly to a kind of that the black China fir saline-alkali tolerant new varieties of hybridization are carried out The method of popularizing planting.
Background technology
Taxodium seeds include 3 kinds of bald cypress, taxodium ascendens and Taxodium mucronatum (referred to as black China fir), originate in North America And Mexico, the advantages that tool growth is fast, form is straight, material is good, water-fast wet, wide adaptability, in Deposits in Eastern Coastal China and inland basin Be with a wide range of applications in the afforestation in area and China carry out woody energy one of emphasis seeds.
" the black China fir of hybridization " has huge quantity increment and increment of organism advantage, future Timber stands, the energy in China Woods, carbon converge woods, leisure woods and forests for water supply conservation construction in terms of have broad application prospects, can be widely used in China's wetland Restoration of the ecosystem, coast protection forest construction and saline land greening afforestation and township afforestation and farm field and forest network etc..
And hybridize the further genralrlization plantation of black China fir, it needs to formulate a set of perfect popularizing planting scheme, to ensure to hybridize Mo Shan ensures the effect of popularizing planting in the survival rate of planting site.
In conclusion a set of perfect popularizing planting method how is formulated for the black China fir saline-alkali tolerant new varieties of hybridization, to protect The black China fir of card hybridization ensures the effect of popularizing planting, becomes those skilled in the art's skill urgently to be resolved hurrily in the survival rate of planting site Art problem.
Invention content
The object of the present invention is to provide a kind of methods carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, for protecting The black China fir of card hybridization ensures the effect of popularizing planting in the survival rate of planting site.
In order to solve the above technical problems, the present invention provides following technical solutions:
A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization, is included the following steps:
(1) hybrid identification is identified from the morphological feature of sample plant, and true using molecular labeling means progress hybrid Reality is identified;
(2) seedling determination records height of seedling and ground diameter number to carrying out seedling determination by the hybrid plant of authenticity identification According to progress seedling stage primary election;
(3) Salt-Tolerance Identification is carried out to selected hybrid plant;
(4) cutting propagation and Experiment of Container nursing are carried out to hybrid plant, using different cutting mediums, different root-promoting hormones And " hybridization black China fir " impact factor that epicormic branch cuttage is taken root is studied in the processing of various concentration and different time, obtains best life Root condition formulates corresponding technical standard, for cultivating seedlings of different size and specifications, selects different nutrient bag, different Culture substrate carries out quantitative criterion metaplasia production, formulates corresponding technical standard;
(5) regional testing carries out multispots trial and growth measurement in target area using parent and filial generation, selects Salt tolerance and the strong high quality tree species of fast-growing character;
(6) saline-alkali soil forestation technical standard is formulated, using different size nursery stock, different planting densities and different types of Nursery stock carries out afforestation experiment.
Preferably, the step (3) includes the following steps:
(31) it using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, chooses Growing way is more several than more consistent material;
(32) equivalent culture substrate is weighed, after measuring substrate water content, above-mentioned experiment material is colonized in plastic flower one by one In basin, it is placed in greenhouse and is cultivated, placement tray under each flowerpot;
(33) after late May plant survives restoration ecosystem, NaCl Stress treatments are carried out 50-60 days, and setting 0,3,6,9, Totally 5 processing are horizontal by 12gkg-1, if 4 repetitions, the solution flowed out when by watering is refunded in flowerpot again, periodic cleaning support The salinity and matrix that are oozed out in the slave flowerpot accumulated in disk are simultaneously refunded in former flowerpot;
(34) carry out plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, proline, The measurement of the physical signs such as the sodium potassium content of root, stem and leaf, analyzes data, evaluates its salt tolerance.
The method provided by the present invention that popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization, rational nursery stock sieve Scheme is selected, evaluate plant salt tolerance by Salt-Tolerance Identification obtains best take root by cutting propagation and Experiment of Container nursing Condition formulates corresponding technical standard, for cultivating seedlings of different size and specifications, selects different nutrient bags, different trainings Matrix is supported, quantitative criterion metaplasia production is carried out, formulates corresponding technical standard, by regional testing, select salt tolerance and fast-growing Property strong high quality tree species, carry out afforestation experiment using different size nursery stock, different planting densities and different types of nursery stock, system Determine saline-alkali soil forestation technical standard, forms a set of perfect popularizing planting scheme, reliable reference data is provided, it is miscellaneous for ensureing It hands over black China fir in the survival rate of planting site, ensures the effect of popularizing planting.
Specific implementation mode
Core of the invention is to provide a kind of method carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, for protecting The black China fir of card hybridization ensures the effect of popularizing planting in the survival rate of planting site.
The method provided by the present invention for carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, includes the following steps:
Step S1, hybrid identification are identified from the morphological feature of sample plant, and miscellaneous using the progress of molecular labeling means Kind authenticity identification;
Step S2, seedling determination record height of seedling and ground diameter to carrying out seedling determination by the hybrid plant of authenticity identification Data carry out seedling stage primary election;
Step S3 carries out Salt-Tolerance Identification to selected hybrid plant;
Step S4 carries out cutting propagation and Experiment of Container nursing to hybrid plant, takes root using different cutting mediums, difference Impact factor that " hybridization black China fir " epicormic branch cuttage is taken root is studied in the processing of hormone and various concentration and different time, is obtained best Condition of taking root, formulate corresponding technical standard, for cultivating seedlings of different size and specifications, select different nutrient bag, no Same culture substrate carries out quantitative criterion metaplasia production, formulates corresponding technical standard;
Step S5, regional testing carry out multispots trial and growth measurement, choosing using parent and filial generation in target area Bring out salt tolerance and the strong high quality tree species of fast-growing character;
Step S6 formulates saline-alkali soil forestation technical standard, using different size nursery stock, different planting densities and not of the same race The nursery stock of class carries out afforestation experiment.
The method provided by the present invention that popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization, rational nursery stock sieve Scheme is selected, evaluate plant salt tolerance by Salt-Tolerance Identification obtains best take root by cutting propagation and Experiment of Container nursing Condition formulates corresponding technical standard, for cultivating seedlings of different size and specifications, selects different nutrient bags, different trainings Matrix is supported, quantitative criterion metaplasia production is carried out, formulates corresponding technical standard, by regional testing, select salt tolerance and fast-growing Property strong high quality tree species, carry out afforestation experiment using different size nursery stock, different planting densities and different types of nursery stock, system Determine saline-alkali soil forestation technical standard, forms a set of perfect popularizing planting scheme, reliable reference data is provided, it is miscellaneous for ensureing It hands over black China fir in the survival rate of planting site, ensures the effect of popularizing planting.
Specifically, the method provided by the present invention for carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, step S3 Include the following steps:
Step S31, using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, It is more several than more consistent material to choose growing way;
Step S32 weighs equivalent culture substrate, and after measuring substrate water content, above-mentioned experiment material is colonized in modeling one by one Expect in flowerpot, is placed in greenhouse and is cultivated, placement tray under each flowerpot;
Step S33 after late May plant survives restoration ecosystem, carries out NaCl Stress treatments 50-60 days, and setting 0,3, 6,9, totally 5 processing are horizontal by 12gkg-1, if 4 repetitions, the solution flowed out when by watering is refunded in flowerpot again, periodically clear It washes the salinity oozed out in the slave flowerpot accumulated in pallet and matrix and refunds in former flowerpot;
Step S34 carries out plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, dried meat ammonia The measurement of the physical signs such as sour, root, stem and leaf sodium potassium content, analyzes data, evaluates its salt tolerance
The method provided by the present invention for carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, is carried out using pot-culture method Evaluation of Salt Tolerance keeps the growth conditions of every plant of experiment material relatively independent, and experimental data easily acquires, with strong points, accuracy Height, experiment use randomized complete-block design, ensure that the data acquisition basis between the district's groups being compared is consistent, data randomness By force, the accuracy for ensureing data analysis, to reach can rational evaluation its salt tolerance, provided accurately for the popularizing planting in later stage Data support purpose.
Above to a kind of method progress that the black China fir saline-alkali tolerant new varieties of hybridization are carried out with popularizing planting provided by the present invention It is discussed in detail.Principle and implementation of the present invention are described for specific case used herein, above example Explanation be merely used to help understand the present invention method and its core concept.It should be pointed out that for the common of the art , without departing from the principle of the present invention, can be with several improvements and modifications are made to the present invention for technical staff, these Improvement and modification are also fallen within the protection scope of the claims of the present invention.

Claims (2)

1. a kind of method carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization, which is characterized in that include the following steps:
(1) hybrid identification is identified from the morphological feature of sample plant, and carries out hybrid authenticity using molecular labeling means Identification;
(2) seedling determination carries out seedling determination, record height of seedling and ground diameter data to the hybrid plant by authenticity identification, into Row seedling stage primary election;
(3) Salt-Tolerance Identification is carried out to selected hybrid plant;
(4) cutting propagation and Experiment of Container nursing are carried out to hybrid plant, using different cutting mediums, different root-promoting hormones and not " the black China fir of hybridization " impact factor that epicormic branch cuttage is taken root is studied with the processing of concentration and different time, obtains best item of taking root Part formulates corresponding technical standard, for cultivating seedlings of different size and specifications, selects different nutrient bags, different cultures Matrix carries out quantitative criterion metaplasia production, formulates corresponding technical standard;
(5) regional testing carries out multispots trial and growth measurement in target area using parent and filial generation, selects salt tolerant Property and the strong high quality tree species of fast-growing character;
(6) saline-alkali soil forestation technical standard is formulated, using different size nursery stock, different planting densities and different types of nursery stock Carry out afforestation experiment.
2. the method for carrying out popularizing planting to the black China fir saline-alkali tolerant new varieties of hybridization as described in claim 1, which is characterized in that institute Step (3) is stated to include the following steps:
(31) using the cuttage seeding of filial generation and parent as experiment material, after the germination of late April cuttage seeding is sprouted, growing way is chosen It is more several than more consistent material;
(32) equivalent culture substrate is weighed, after measuring substrate water content, above-mentioned experiment material is colonized in plastic flowerpot one by one, It is placed in greenhouse and is cultivated, placement tray under each flowerpot;
(33) after late May plant survives restoration ecosystem, NaCl Stress treatments are carried out 50-60 days, and setting 0,3,6,9,12g Totally 5 processing are horizontal by kg-1, if 4 repetitions, the solution flowed out when by watering is refunded in flowerpot again, periodic cleaning pallet inner product The salinity and matrix that are oozed out in the slave flowerpot deposited simultaneously are refunded in former flowerpot;
(34) plant height, ground diameter, above and below the ground biomass estimation, measuring chlorophyll content, propylene glycol, proline, rhizome are carried out The measurement of the physical signs such as the sodium potassium content of leaf, analyzes data, evaluates its salt tolerance.
CN201710311157.XA 2017-05-05 2017-05-05 A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization Pending CN108781950A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476635A (en) * 2019-09-03 2019-11-22 南通大学 The sticking device and its screening technique of forest screening salt-enduring cultivars difficult to take root

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CN101341852A (en) * 2007-08-10 2009-01-14 江苏省中国科学院植物研究所 Cross breeding method for black cypress and southern cypress
CN103430784A (en) * 2013-08-23 2013-12-11 江苏沿江地区农业科学研究所 Simple method for evaluating salt tolerance of crops at seedling stage
CN209151750U (en) * 2018-11-12 2019-07-26 重庆市林森耐水中山杉研发有限公司开州分公司 A kind of cuttage and seedling culture greenhouse of multi-functional middle mountain China fir

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101341852A (en) * 2007-08-10 2009-01-14 江苏省中国科学院植物研究所 Cross breeding method for black cypress and southern cypress
CN103430784A (en) * 2013-08-23 2013-12-11 江苏沿江地区农业科学研究所 Simple method for evaluating salt tolerance of crops at seedling stage
CN209151750U (en) * 2018-11-12 2019-07-26 重庆市林森耐水中山杉研发有限公司开州分公司 A kind of cuttage and seedling culture greenhouse of multi-functional middle mountain China fir

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476635A (en) * 2019-09-03 2019-11-22 南通大学 The sticking device and its screening technique of forest screening salt-enduring cultivars difficult to take root

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Application publication date: 20181113