CN108770515B - Seedling picking method for strawberry cutting seedling and application thereof - Google Patents
Seedling picking method for strawberry cutting seedling and application thereof Download PDFInfo
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- CN108770515B CN108770515B CN201810474655.0A CN201810474655A CN108770515B CN 108770515 B CN108770515 B CN 108770515B CN 201810474655 A CN201810474655 A CN 201810474655A CN 108770515 B CN108770515 B CN 108770515B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The invention belongs to the technical field of plant breeding, and particularly discloses a seedling picking method for strawberry cutting seedling and application thereof. The seedling picking method comprises the steps of picking seedlings when the seedlings have 2-3 leaves and 1 heart and the base of a new stem is provided with 0.5-1 cm of white adventitious root; when the seedlings are picked, the seedlings are cut off from the stolons by clinging to the base parts of the shortened new stems of the seedlings, the stolons in front of and behind the seedlings are not cut off, and the stolons in front of and behind the seedlings after the seedlings are picked are connected through the base parts of the shortened new stems of the residual seedlings, so that the stolons can continue to grow normally and can generate new seedlings, and the seedlings can be continuously picked from the single stolons. The method solves the problems of complex process, low seedling quality and low seedling raising efficiency of the strawberry seedling picking method in the prior art, realizes the continuous picking of high-quality seedlings from strawberry stolons, reduces the labor intensity and improves the cutting survival rate and the seedling raising efficiency.
Description
Technical Field
The invention belongs to the technical field of plant breeding, and particularly relates to a seedling picking method for strawberry cutting seedling and application thereof.
Background
The strawberry is a perennial herb plant of the genus strawberry of the family rosaceae, the strawberry widely cultivated in the production belongs to the octaploid pineapple strawberry (Fragaria x ananasa Duch.), the stolons of the strawberry are thin and long in internodes, after the stolons germinate, short shrubs and leaves grow upwards on the second part, adventitious roots grow downwards to form stolon seedlings, the axillary parts of the newly formed stolon seedlings continue to germinate the stolons and form next-stage stolon seedlings, and the stolon seedlings can completely inherit the variety characters of mother seedlings, which is the basic principle of strawberry seedling breeding by using the stolons.
At present, the seedlings from strawberry stolons are obtained mainly by two modes, namely a tendril pressing method and a cutting method in production. A vine pressing method: along with the elongation of the stolons, pressing tendrils at even-numbered nodes of the stolons to promote the seedlings to take roots, and the stolons among all levels of seedlings are not cut off, so that the seedlings can obtain nutrition from mother seedlings through the stolons; however, stolons are mutually wound, the labor intensity of tendril pressing operation is high, large and small seedlings are mixed together, and the cultivation of high-quality strong seedlings is not facilitated. A cutting method comprises the following steps: during the growth period of the stolons, tendril pressing operation is not carried out, when 3-4 seedlings with a small amount of adventitious roots grow on each stolon, the stolons at the two ends of each seedling are cut off, the obtained seedlings are classified according to size and then are subjected to concentrated cutting for rooting, the labor intensity is favorably reduced, and the uniformity of the seedlings is improved; however, because of different seedling ages of the seedlings, the leaves of the larger seedlings are too many, the adventitious roots are exposed for too long and necrotize, and the leaves and the adventitious roots of the smaller seedlings are too few, so that the smaller seedlings are not beneficial to survival.
Chinese patent application publication No. CN103975730A discloses a method for raising strawberry seedlings by cutting, but only stolon seedlings with 2-3 unfolded leaves are selected, and the lower ends of the stolon seedlings are cut into wedges for cutting. The Chinese patent application with publication number CN104509363A discloses a two-stage seedling growing method for strawberries, which comprises cutting off stolons with 3-4 seedlings, removing redundant leaves of the seedlings, and only keeping 2 leaves and 1 heart of the seedlings for cuttage. Chinese patent application publication No. CN106258368A discloses a novel method for efficient and rapid seedling formation of strawberry stolons, which is divided into early and newly formed seedlings according to the freshness of the adventitious roots of the seedlings, the newly formed seedlings can be directly used for cuttage, and the seedlings formed in the early stage need to be used for cuttage after the necrotic adventitious roots are removed and healthy tissues can be seen.
However, the seedling age difference of all levels of creeping stem seedlings of strawberries is large, the conventional seedling picking method needs to grade the seedlings, remove leaves and repair roots of the seedlings with large seedling age, finely manage the seedlings with small seedling age, have complicated picking process, low seedling quality, large management difficulty after cuttage, and only pick 3-4 seedlings on each creeping stem, and have low cuttage seedling raising efficiency.
Disclosure of Invention
In order to solve the problems of complicated process, low seedling quality and low seedling raising efficiency of the strawberry seedling raising method in the prior art, the invention aims to provide the strawberry cutting seedling raising method and the application thereof.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
in a first aspect, the invention provides a seedling picking method for strawberry cutting seedling raising, wherein during seedling picking, the seedlings are cut off from the stolons by being tightly attached to the shortened new stem base parts of the seedlings, the stolons before and after the seedlings are not cut off, and the stolons before and after the seedlings are picked are connected through the residual shortened new stem base parts of the seedlings.
Preferably, when the seedling is 2-3 leaves and 1 heart and the base of the new stem is provided with 0.5-1 cm white adventitious root, the seedling is collected.
The seedling picking method does not damage the stolons, the stolons are still connected with the mother seedlings and continue to grow, and new seedlings can be generated, so that the seedlings can be picked continuously from the single stolons, and only one seedling is grown on the single stolons all the time.
Preferably, after the stolons of the mother seedlings germinate, the stolons tip is horizontally, downwardly or upwardly grown through manual intervention.
The seedling picking method provided by the invention specifically comprises the following steps:
(1) stolon management: after the stolons of the mother seedlings germinate, the stolons grow horizontally, downwards or upwards through manual intervention.
(2) Harvesting the seedlings: when the stolon seedlings grow to 2-3 leaves and 1 heart and have 0.5-1 cm white adventitious roots, the stolon seedlings are tightly attached to the shortened new stem base parts of the stolon seedlings and cut off from the stolon seedlings, the white adventitious roots of 0.5-1 cm are arranged at the new stem base parts, and the stolon seedlings before and after the stolon seedlings are still normally connected through the residual shortened new stem base parts of the seedlings. And (4) completely healing the cut wound after one day, and harvesting according to the same method when the next-stage stolon seedlings grow to 2-3 leaves and 1 heart and have 0.5-1 cm white adventitious roots. The collected seedling is subjected to cuttage culture to become the strawberry original seedling or the seedling for production.
In a second aspect, the invention provides application of the seedling picking method in strawberry cutting seedling.
The seedlings collected by the seedling collection method are 2-3 leaves and 1 heart, the base part of the new stem is provided with 0.5-1 cm of white adventitious root, the size is uniform, the seedlings are easy to survive after cuttage, the seedlings can become the original strawberry seedlings or the seedlings for production after seedling cultivation, and the labor intensity of cuttage seedling culture can be effectively reduced.
Therefore, the invention also provides the application of the seedling collection method in the preparation of the strawberry original seedlings or the seedlings for production.
The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.
The invention has the beneficial effects that:
the strawberry seedling picking method can be used for picking strawberry seedlings, only one seedling growing on stolons connected with mother seedlings can always be picked, the quick growth of the seedling and the management of the stolons are facilitated, the picked seedlings are 2-3 leaves and 1 heart and are provided with 0.5-1 cm adventitious roots, the sizes are uniform, the seedlings are easy to survive after cuttage, the strawberry original seedlings or the seedlings for production can be obtained after seedling cultivation, and the labor intensity of cuttage seedling cultivation can be effectively reduced.
Furthermore, the seedling collection method of the present invention does not cut off the stolons before and after the seedlings, the stolons are still connected with the mother seedlings and continue to grow, and new seedlings can be generated, so that the seedlings can be continuously collected from the single stolons. In 4-9 months, more than 9 seedlings can be harvested from the single stolons at most in the seedling culture period, and compared with the traditional seedling culture mode, the cutting seedling culture efficiency can be improved by more than 2 times.
Drawings
FIG. 1 is a schematic view of a seedling collection method in the case of mother seedling stolon shoot horizontal growth management;
FIG. 2 is a schematic view of a seedling picking method in the case of mother seedling stolon tip downward growth management;
FIG. 3 is a schematic view of a seedling picking method in the case of mother seedling stolon shoot upward growth management;
in the figure, 1: mother seedling, 2: stolons, 3: stolon tips, 4: stolon seedlings, 5: shortened base of new stem of stolon seedling, 6: shortened base of new stem remaining on stolons, 7: adventitious roots, 8: shortened new stem, 9: stolon fixation clamp.
Detailed Description
The present invention is further illustrated by the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
The seedling picking process in the case of mother seedling creeping stem tip horizontal growth management is explained based on the actual cultivation test and with reference to fig. 1.
(1) Stolon management: the mother seedlings 1 are fixedly planted on one side of the elevated cultivation bed, and the creeping stem tips 3 generated from the mother seedlings freely grow on the cultivation bed along the horizontal direction. Properly guiding the growth direction of the stolon tips 3 in the horizontal direction during seedling picking, and ensuring that the stolon tips are always on the cultivation bed.
(2) Harvesting the seedlings: when the stolon seedlings 4 grow to 2-3 leaves with 1 heart and have 0.5-1 cm white adventitious roots 7, the stolon seedlings are tightly attached to the short new stem base parts 5 and cut off from the stolon 2 for cuttage, the short new stem base parts 5 are provided with 0.5-1 cm white adventitious roots 7, the stolon 2 before and after the short new stem base parts are still normally connected through the short new stem base parts 6 remained on the stolon, and the cut wounds of the short new stem base parts 6 remained on the stolon are completely healed after one day. With the increase of the number and the length of the stolons, the seedlings 4 with 2-3 leaf 1 cores and 0.5-1 cm white adventitious roots 7 on the stolons 2 are picked in time. After seedling picking, the strawberry seedlings are inserted into 32-hole plug trays filled with the matrix and cultured for 20 days to form high-quality strawberry seedlings.
During the seedling culture period of 4-9 months per year, more than 9 seedlings can be harvested from the single stolons, the seedling culture efficiency is more than 3 times of that of the traditional seedling culture mode, only the stolons need to be properly guided during the seedling culture period, the time and the labor are saved, the harvested seedlings are shortened, the new stems 8 are L-shaped, and the cuttage fixation and the rooting are facilitated.
Example 2
The seedling picking process when the stolon stem tip of the mother seedling grows and manages downwards is explained based on the actual cultivation test and by combining with the figure 2.
(1) Stolon management: the mother seedlings 1 are planted in the overhead cultivation tank, and the creeping stem tips 3 generated from the mother seedlings freely grow downwards under the action of gravity. In order to prevent the stolon tips 3 from contacting the ground, stolon 2 close to the side of the mother seedling 1 is wound and fixed in time.
(2) Harvesting the seedlings: when the stolon seedlings 4 grow to 2-3 leaves with 1 heart and have 0.5-1 cm white adventitious roots 7, the stolon seedlings are tightly attached to the short new stem base parts 5 and cut off from the stolon 2, the short new stem base parts 5 are provided with 0.5-1 cm white adventitious roots 7, the stolon 2 before and after the short new stem base parts are still normally connected through the short new stem base parts 6 remained on the stolon, and the cut wounds of the short new stem base parts 6 remained on the stolon are completely healed after one day. With the increase of the number and the length of the stolons, the seedlings 4 with 2-3 leaf 1 cores and 0.5-1 cm white adventitious roots 7 on the stolons 2 are picked in time. After seedling picking, the strawberry seedlings are inserted into 32-hole plug trays filled with the matrix and cultured for 20 days to form high-quality strawberry seedlings.
During the seedling raising period of 4-9 months, more than 9 seedlings are harvested from the single stolon at most, and the seedling raising efficiency is more than 3 times of that of the traditional seedling picking mode. Because the stolon tips 3 vertically grow downwards under the action of gravity, the shortened new stems 8 formed by the seedlings are J-shaped, the manual seedling picking is convenient, and the labor intensity of seedling picking is reduced.
Example 3
The seedling picking process in the case of mother seedling creeping stem tip upward growth management is explained based on the actual cultivation test and with reference to fig. 3.
(1) Stolon management: the mother seedlings 1 are fixedly planted on one side of the overhead cultivation bed, and the stolon tips 3 generated from the mother seedlings keep growing vertically upwards under the fixation of the stolon fixing clamps 9.
(2) Harvesting the seedlings: when the stolon seedlings 4 grow to 2-3 leaves with 1 heart and have 0.5-1 cm white adventitious roots 7, the stolon seedlings are tightly attached to the short new stem base parts 5 and cut off from the stolon 2, the short new stem base parts 5 are provided with 0.5-1 cm white adventitious roots 7, the stolon 2 before and after the short new stem base parts are still normally connected through the short new stem base parts 6 remained on the stolon, and the cut wounds of the short new stem base parts 6 remained on the stolon are completely healed after one day. After seedling picking, the stolon tips 3 are fixed on the stolon fixing clamps 9 in time, the stolon tips are kept growing vertically upwards, and when the stolon tips grow to 2-3 leaves and 1 heart and have 0.5-1 cm white adventitious roots 7, the stolon tips are cut off according to the same method. After seedling picking, the strawberry seedlings are inserted into 32-hole plug trays filled with the matrix and cultured for 20 days to form high-quality strawberry seedlings.
During the seedling raising period of 4-9 months, more than 9 seedlings are harvested from the single stolon at most, and the seedling raising efficiency is more than 3 times of that of the traditional seedling picking method. Because the stolon tips 3 vertically grow upwards under the fixation of the stolon fixing clamps 9, the shortened new stems 8 formed by the seedlings are I-shaped, which is beneficial to realizing mechanical seedling collection and improving the seedling collection efficiency.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (4)
1. A seedling picking method for strawberry cutting seedling is characterized in that when the seedlings are 2-3 leaves and 1 heart and the new stem base is provided with a white adventitious root of 0.5-1 cm, the seedlings are cut off from stolons by clinging to the shortened new stem base of the seedlings without cutting the stolons before and after the seedlings, so that the stolons before and after the seedlings are picked are connected through the residual shortened new stem base of the seedlings;
and continuously harvesting the seedlings from the single stolons, wherein only one seedling growing on the single stolons is always grown.
2. The seedling collection method according to claim 1, wherein the stolon tips generated from the mother seedlings are grown horizontally, downwardly or upwardly by artificial management.
3. The application of the seedling collection method of claim 1 or 2 in strawberry cutting seedling.
4. The use of the seedling collection method according to claim 1 or 2 for preparing a strawberry original seedling or a seedling for production, wherein the seedling collected by the seedling collection method according to any one of claims 1 or 2 is used as the original seedling or the seedling for production after cutting culture.
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| CN109964755B (en) * | 2019-05-15 | 2021-03-23 | 十堰市经济作物研究所 | Method for improving survival rate and quality of strawberry stock seedlings |
| CN112930890B (en) * | 2021-02-03 | 2022-10-14 | 农业农村部规划设计研究院 | Strawberry stolon traction and cutting composite seedling culture method |
| CN113099976B (en) * | 2021-04-12 | 2023-12-05 | 云南省农业科学院园艺作物研究所 | Method for efficiently breeding seedlings by creeping stems of open field strawberries |
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| CN102550265B (en) * | 2012-02-13 | 2015-07-08 | 张进峰 | Method for strawberry seedling breeding |
| CN105075599A (en) * | 2015-08-10 | 2015-11-25 | 合肥槟阳农业科技有限公司 | Method for breeding strawberry seedlings |
| CN106258368A (en) * | 2016-08-11 | 2017-01-04 | 安徽省农业科学院园艺研究所 | A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon |
| CN107593405A (en) * | 2017-09-30 | 2018-01-19 | 江苏农林职业技术学院 | The synthesis mating system of strawberry seedling |
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