CN108751646A - A kind of preparation method of biological deodorant - Google Patents
A kind of preparation method of biological deodorant Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods of biological deodorant, belong to biological field.The protective agent as deodorant activities bacterium such as trehalose of the present invention, skimmed milk, starch Sodium Octenyl Succinate, beta-cyclodextrin; glycerine can form hydrogen bond with somatic cells film and protein; the rate-of-loss of coolant of cell can be slowed down; extend the activity time of thalline; trehalose is under various severe environmental conditions; there is good protective effect, skimmed milk and beta-cyclodextrin protective film can be formed outside thalline for cell membrane and cellular component matter protein, nucleic acid to organism etc., keep microbial activity.The present invention solves the problems, such as that the single strain activity added in current biological deodorant is low, deodorizing effect is poor.
Description
Technical field
The invention belongs to biological fields, and in particular to a kind of preparation method of biological deodorant.
Background technology
With the fast development of China's economy, people are gradually changed from material life to cultural life, and tourist industry is able to soon
Speed development, scenic spot public lavatory quantity is on the increase, the control of scenic spot lavatory stench and the processing of excrement have become influence scenic spot can
An important factor for sustainable development.Every year, urban and rural domestic refuse yield in China's is about 3.7 hundred million tons, these house refuses are in rubbish
Gathering station is stacked, generates a large amount of stenches in transport process, and serious environmental pollution is caused.Stench contain there are many it is poisonous and harmful at
Point, wherein ammonia, hydrogen sulfide, mercaptan and methyl mercaptan etc. are main pernicious gas ingredients, and the discharge of stench can make surrounding enviroment
At serious pollution, cause serious injury to health after people's sucking for a long time.Therefore implement odor pollution control and management, open
The research of exhibition removing foul gas is of great significance.
Biological deodorization method is the method for the processing foul smell to grow up the 1950s, and this method is since treatment effeciency is high, nothing
The features such as secondary pollution, simple, low-cost and easy to operation processing equipment, it has also become the hot spot of many country's researchs.Biology
The core of deodorization is efficient deodorizing functional microorganism microbial inoculum, passes through separation screening efficient deodorizing function stem and optimum organization compatibility
At microbial deoderizer, it is inoculated in the stench intensity that can effectively reduce its release in foul smell environment, reaches stench original position
Control purpose.
Currently, deodorization functions microbial bacterial agent mainly selects the bacteriums such as Escherichia, brevibacterium and bacillus logical
Dry cell of microorganism deodorant is made in everfermentation, heat sterilization, although there is certain effect, single strain due to its metabolic type,
Type of respiration and action function are single, weak to adaptive capacity to environment, it is difficult to become dominant microflora in foul smell source of release, cause to remove
Smelly effect is unsatisfactory.Therefore, producing a kind of deodorant of higher effect has the prodigious market demand.
Invention content
The technical problems to be solved by the invention：The low, deodorization for the single strain activity added in current biological deodorant
The problem of effect difference provides a kind of preparation method of biological deodorant.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of biological deodorant, which is characterized in that the preparation method includes the following steps：
（1）According to the mass fraction, 8 ~ 10 parts of fowl and livestock farm soil, 3 ~ 7 parts of activated sludge, 6 ~ 9 parts of landfill leachates, 3 ~ 5 are taken
The corrupt stalk mixing of part, must mix screening raw material, mixing is taken to screen raw material in mass ratio 1：8 ~ 10 are added physiological saline, are diluted to
10-6Dilution grade, obtain dilution, dilution taken to be coated on successively except ammonia bacterial strain screening culture medium, correspond respectively to 28 ~ 35 DEG C training
Support, 23 ~ 28 DEG C of cultures, 25 ~ 33 DEG C of cultures, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, be named as J-1, J-2,
J-3 takes J-1, J-2, J-3 in mass ratio 1 ~ 3：2~5：1 ~ 3 mixing, must mix and remove ammonia bacterium, take mixing to remove ammonia bacterium and connect by 3 ~ 5%
Kind amount is seeded to except centrifugation is cultivated in ammine selective culture medium, is taken pellet frozen to dry, is obtained freeze-drying object；
（2）Take dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, correspond respectively to 28 ~ 35 DEG C culture, 23 ~ 28 DEG C
Culture, 25 ~ 33 DEG C of cultures, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as S-1, S-2, S-3, take S-1,
S-2, S-3 in mass ratio 2 ~ 5：1~4：3 ~ 6 mixing, must mix vulcanisation hydrogen bacterium, take and mix vulcanisation hydrogen bacterium by 3 ~ 5% inoculation
Amount, which is seeded in vulcanisation hydrogen selective medium, cultivates, and centrifuges, takes pellet frozen to dry, must be freeze-dried object a, takes freezing dry
Dry object in mass ratio 1 ~ 4：3 ~ 5 are added a mixing of freeze-drying object, obtain deodorant base material；
（3）According to the mass fraction, take 5 ~ 8 portions of trehaloses, 10 ~ 15 portions of skimmed milks, 2 ~ 4 parts of starch Sodium Octenyl Succinates, 4 ~ 6 parts
Beta-cyclodextrin, 4 ~ 7 parts of glycerine, 0.3 ~ 0.8 part of vitamin C, 0.4 ~ 0.7 part of xanthans, 8 ~ 10 portions of mannitol, 7 ~ 12 parts of lactose are mixed
It closes, obtains mixture, take deodorant matrix object in mass ratio 5 ~ 8：3~5：10 ~ 15 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization
Matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1100 ~ 1200 DEG C of heat preservations, is cooled to room temperature, obtains cooling thing, take aggregate
Diatomite in mass ratio 5 ~ 7：6 ~ 9 are added cooling thing, add the zirconia ball of 2 ~ 3 times of cooling thing quality, ball milling is dry, powder
Broken sieving is collected sieving particle, is sent out by quality, and 1 ~ 4 part of paraffin, 2 ~ 5 parts of clays, 1 ~ 3 part of sawdust, 20 ~ 30 parts of sieving particles are taken
Object mixes, water spray 3 ~ 4h of ageing, compression molding, and calcining is cooled to room temperature, obtains cooling thing a, takes cooling thing a in mass ratio 3 ~ 5：1
~ 3 are added deodorization matrix object to get biological deodorant.
The step（1）In except ammonia bacterial strain screening culture medium be：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：
80 ~ 90 sequentially add beef extract-peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, successively
To corresponding bacteria screening culture medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min to get.
The step（1）In remove ammine selective culture medium：Except ammine selective culture medium：According to the mass fraction, 40 ~ 50 parts are taken
Ammonium hydroxide, 1 ~ 3 part of KH of sucrose, 10 ~ 15 parts of mass fractions for 25%2PO4, 0.3 ~ 0.7 part of MgSO4·7H2O, 0.1 ~ 0.3 part
FeSO4 , 0.001 ~ 0.003 part of ZnSO4 , 1 ~ 3 part of NaCl, 1000 parts of water, 121 DEG C of sterilizing 20min.
The step（2）In vulcanisation hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：4 ~ 6 sequentially add
Beef extract-peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacterium successively
Screening and culturing medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min.
The step（2）In vulcanisation hydrogen selective medium：According to the mass fraction, 3 ~ 6 parts of glucose, 0.3 ~ 0.6 are taken
Part K2HPO4, 1 ~ 3 part of KNO3, 0.3 ~ 0.7 part of MgCl2, 0.3 ~ 0.6 part of NaCl, 0.3 ~ 0.6 part of NH4Cl, 1 ~ 3 part of Na2CO3、0.01
~ 0.03 part of FeCl2, 1000 parts of water, pH is naturally, 121 DEG C of 15 min of sterilizing.
The step（3）Middle auxiliary microbial inoculum：According to the mass fraction, take 1 ~ 4 part of streptomyces microflavus, 2 ~ 6 parts of bacillus subtilises, 1 ~
3 parts of Mucor circinelloides, 4 ~ 7 parts of bacillusmusilaginosiengineerings, 6 ~ 9 parts of double qi Bacillus acidi lacticis, 6 ~ 8 parts of cloth Laplace saccharomycete.
The step（4）The calcination condition of middle cooling thing a is：In 250 ~ 300 DEG C of dryings, then at 900 ~ 1000 DEG C of calcinings 20
~30min。
Compared with other methods, advantageous effects are the present invention：
（1）The present invention regard the fowl and livestock farm soil of high factor containing foul smell, activated sludge etc. as screening raw material, respectively with ammonia
As carbon source, screening respectively obtains high tolerance ammonia, the bacterium of hydrogen sulfide, actinomyces, fungi, is mixed, obtains for water, hydrogen sulfide
It is nitrite nitrogen and nitrate nitrogen by ammonium oxidation to the base activated bacterium of deodorization, under aerobic conditions, nitrite bacteria is by NH4 +-
N is oxidized to NO2- N, then by nitrate bacteria by NO2 -- N is oxidized to NO3- N utilizes CO2、CO3 2-、HCO3 - Deng be used as carbon source, pass through
With NH3, NH4 +- N or NO2 -The redox reaction of-N obtains energy, and the desulfurization microorganism filtered out is the metabolism by itself
Effect converts the sulfide in stench to elemental sulfur or sulfate, and the metabolism of microorganism itself makes odor pollutant be degraded to
Requirement of the active bacteria that odorless, harmless final product, such as H2O, CO2 reach deodorization purpose, and filter out to environmental condition
Low, the energy needed for growth and breeding is the energy of oxidation generated in oxidation decomposition course, does not need other nutriments, reduces
Operating cost is low；
（2）The protection as deodorant activities bacterium such as trehalose of the present invention, skimmed milk, starch Sodium Octenyl Succinate, beta-cyclodextrin
Agent, glycerine can form hydrogen bond with somatic cells film and protein, can slow down the rate-of-loss of coolant of cell, when extending the activity of thalline
Between, under various severe environmental conditions, cell membrane and cellular component matter protein, nucleic acid to organism etc. have trehalose
Good protective effect, skimmed milk and beta-cyclodextrin can form protective film outside thalline, keep microbial activity；
（3）The diatomite of a part of precalcining processing is added in the present invention, can effectively remove the impurity such as the organic matter in diatomite, make
It forms uniformly apparent reticular structure, forms it into better nano-pore structure, is added in diatomite aggregate, adds and make
The sawdust in hole, clay of bonding etc., form the fixation support of deodorizing microorganism, and diatomite itself is a kind of with nanometer micropore
Porous structure, porosity reach submicron order, and oxygen can be entered by it in deeper stomata, have larger specific surface area and
Containing the associate hydrogen bond largely with hydrogen bond in its duct, thalline can be adsorbed by the effect of hydrogen bond and Van der Waals force, and by nutrition
In mass transfer to stomata, the activity of microorganism is stimulated, while by adsorbing the free molecule in foul smell into blowhole, by micro- life
Object decomposes, and does not easily cause diffusion limitation, improves the utilization rate of thalline.
Specific implementation mode
Except ammonia bacterial strain screening culture medium：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：80 ~ 90 sequentially add ox
Meat extract peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium obtain corresponding bacterium sieve successively
Select culture medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min to get.
Vulcanisation hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：4 ~ 6 sequentially add beef extract-peptone
Solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening culture medium, put successively
Line bacterium screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min.
Except ammine selective culture medium：According to the mass fraction, the ammonia that 40 ~ 50 portions of sucrose, 10 ~ 15 parts of mass fractions are 25% is taken
Water, 1 ~ 3 part of KH2PO4, 0.3 ~ 0.7 part of MgSO4·7H2O, 0.1 ~ 0.3 part of FeSO4 , 0.001 ~ 0.003 part of ZnSO4 , 1 ~ 3 part
NaCl, 1000 parts of water, 121 DEG C of sterilizing 20min.
Vulcanisation hydrogen selective medium：According to the mass fraction, 3 ~ 6 parts of glucose, 0.3 ~ 0.6 part of K are taken2HPO4, 1 ~ 3 part
KNO3, 0.3 ~ 0.7 part of MgCl2, 0.3 ~ 0.6 part of NaCl, 0.3 ~ 0.6 part of NH4Cl, 1 ~ 3 part of Na2CO3, 0.01 ~ 0.03 part of FeCl2、
1000 parts of water, pH is naturally, 121 DEG C of 15 min of sterilizing.
Assist microbial inoculum：According to the mass fraction, 1 ~ 4 part of streptomyces microflavus, 2 ~ 6 parts of bacillus subtilises, 1 ~ 3 part of volume branch Mucor are taken
Bacterium, 4 ~ 7 parts of bacillusmusilaginosiengineerings, 6 ~ 9 parts of double qi Bacillus acidi lacticis, 6 ~ 8 parts of cloth Laplace saccharomycete.
A kind of preparation method of biological deodorant, includes the following steps：
（1）According to the mass fraction, 8 ~ 10 parts of fowl and livestock farm soil, 3 ~ 7 parts of activated sludge, 6 ~ 9 parts of landfill leachates, 3 ~ 5 are taken
The corrupt stalk mixing of part, must mix screening raw material, mixing is taken to screen raw material in mass ratio 1：8 ~ 10 are added physiological saline, are diluted to
10-6Dilution grade, obtain dilution, dilution taken to be coated on successively except ammonia bacterial strain screening culture medium, correspond respectively to 28 ~ 35 DEG C training
It supports to cultivate within 2 ~ 3 days, 23 ~ 28 DEG C 3 ~ 5 days, 25 ~ 33 DEG C and cultivate 1 ~ 3 day, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter,
It is named as J-1, J-2, J-3, takes J-1, J-2, J-3 in mass ratio 1 ~ 3：2~5：1 ~ 3 mixing, must mix and remove ammonia bacterium, mixing is taken to remove
Ammonia bacterium is seeded to by 3 ~ 5% inoculum concentration except in ammine selective culture medium, is cultivated 3 ~ 5 days in 25 ~ 30 DEG C, 180r/min, centrifugation,
It takes pellet frozen to dry, obtains freeze-drying object；
（2）Take dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, correspond respectively to 28 ~ 35 DEG C cultivate 2 ~ 3 days, 23
~ 28 DEG C cultivate 3 ~ 5 days, 25 ~ 33 DEG C cultivate 1 ~ 3 day, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, be named as S-1,
S-2, S-3 take S-1, S-2, S-3 in mass ratio 2 ~ 5：1~4：3 ~ 6 mixing, must mix vulcanisation hydrogen bacterium, take the vulcanisation hydrogen of mixing
Bacterium is seeded to by 3 ~ 5% inoculum concentration in vulcanisation hydrogen selective medium, is cultivated 3 ~ 5 days in 25 ~ 30 DEG C, 180r/min, from
The heart takes pellet frozen to dry, and must be freeze-dried object a, takes freeze-drying object in mass ratio 1 ~ 4：3 ~ 5, which are added freeze-drying object a, mixes
It closes, obtains deodorant base material；
（3）According to the mass fraction, take 5 ~ 8 portions of trehaloses, 10 ~ 15 portions of skimmed milks, 2 ~ 4 parts of starch Sodium Octenyl Succinates, 4 ~ 6 parts
Beta-cyclodextrin, 4 ~ 7 parts of glycerine, 0.3 ~ 0.8 part of vitamin C, 0.4 ~ 0.7 part of xanthans, 8 ~ 10 portions of mannitol, 7 ~ 12 parts of lactose are mixed
It closes, obtains mixture, take deodorant matrix object in mass ratio 5 ~ 8：3~5：10 ~ 15 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization
Matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1100 ~ 1200 DEG C of 1 ~ 2h of heat preservation, is cooled to room temperature, obtains cooling thing, take
Aggregate diatomite in mass ratio 5 ~ 7：6 ~ 9 are added cooling things, add the zirconia ball of 2 ~ 3 times of cooling thing quality, and ball milling 20 ~
30min dry, pulverize and sieve with 100 mesh sieve, and collects sieving particle, is sent out by quality, take 1 ~ 4 part of paraffin, 2 ~ 5 parts of clays, 1 ~ 3 part of wood
Bits, 20 ~ 30 parts of sieving particulate matter mixing, water spray 3 ~ 4h of ageing, compression molding, in 250 ~ 300 DEG C of dryings, then at 900 ~ 1000 DEG C
20 ~ 30min is calcined, is cooled to room temperature, obtains cooling thing a, take cooling thing a in mass ratio 3 ~ 5：1 ~ 3 be added deodorization matrix object to get
Biological deodorant.
Except ammonia bacterial strain screening culture medium：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：80 sequentially add beef
Cream peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening successively
Culture medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C sterilizing 20min to get.
Vulcanisation hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：4, which sequentially add beef extract-peptone, consolidates
Body culture medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening culture medium, unwrapping wire successively
Bacterium screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min.
Except ammine selective culture medium：According to the mass fraction, the ammonium hydroxide, 1 part that 40 portions of sucrose, 10 parts of mass fractions are 25% are taken
KH2PO4, 0.3 part of MgSO4·7H2O, 0.1 part of FeSO4 , 0.001 part of ZnSO4 , 1 part of NaCl, 1000 parts of water, 121 DEG C of sterilizings
20min。
Vulcanisation hydrogen selective medium：According to the mass fraction, 3 parts of glucose, 0.3 part of K are taken2HPO4, 1 part of KNO3、0.3
Part MgCl2, 0.3 part of NaCl, 0.3 part of NH4Cl, 1 part of Na2CO3, 0.01 part of FeCl2, 1000 parts of water, pH is naturally, 121 DEG C of sterilizings 15
min。
Assist microbial inoculum：According to the mass fraction, 1 part of streptomyces microflavus, 2 parts of bacillus subtilises, 1 part of Mucor circinelloides, 4 parts of glue are taken
Freeze sample bacillus, 6 parts of double qi Bacillus acidi lacticis, 6 parts of cloth Laplace saccharomycete.
A kind of preparation method of biological deodorant, includes the following steps：
（1）According to the mass fraction, 8 parts of fowl and livestock farm soil, 3 parts of activated sludge, 6 parts of landfill leachates, 3 parts of corrupt stalks are taken
Mixing must mix screening raw material, take mixing screening raw material in mass ratio 1：8 are added physiological saline, are diluted to 10-6Dilution grade,
Dilution is obtained, dilution is taken to be coated on successively except ammonia bacterial strain screening culture medium, 28 DEG C is corresponded respectively to and cultivates 2 days, 23 DEG C of cultures 3
It, 25 DEG C cultivate 1 day, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as J-1, J-2, J-3, take J-1, J-
2, in mass ratio 1 J-3：2：1 mixing, must mix and remove ammonia bacterium, take mixing except ammonia bacterium is seeded to by 3% inoculum concentration except ammine selective
It in culture medium, is cultivated 3 days in 25 DEG C, 180r/min, centrifugation takes pellet frozen to dry, obtains freeze-drying object；
（2）It takes dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, corresponds respectively to 28 DEG C and cultivate 2 days, 23 DEG C of trainings
Support 3 days, 25 DEG C cultivate 1 day, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as S-1, S-2, S-3, take S-1,
S-2, S-3 in mass ratio 2：1：3 mixing, must mix vulcanisation hydrogen bacterium, and the vulcanisation hydrogen bacterium of mixing is taken to be seeded to by 3% inoculum concentration
It in vulcanisation hydrogen selective medium, is cultivated 3 days in 25 DEG C, 180r/min, centrifugation takes pellet frozen to dry, must be freeze-dried
Object a takes freeze-drying object in mass ratio 1：3 are added a mixing of freeze-drying object, obtain deodorant base material；
（3）According to the mass fraction, take 5 portions of trehaloses, 10 portions of skimmed milks, 2 parts of starch Sodium Octenyl Succinates, 4 parts of beta-cyclodextrins,
4 parts of glycerine, 0.3 part of vitamin C, 0.4 part of xanthans, 8 portions of mannitol, 7 parts of lactose mixing, obtain mixture, take deodorant matrix
Object in mass ratio 5：3：10 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1100 DEG C of heat preservation 1h, is cooled to room temperature, obtains cooling thing, take aggregate diatom
Soil in mass ratio 5：6 are added cooling thing, add the zirconia ball of 2 times of cooling thing quality, ball milling 20min dry, pulverize
100 mesh sieve, and collect sieving particle, are sent out by quality, take 1 part of paraffin, 2 parts of clays, 1 part of sawdust, 20 parts of sieving particulate matter mixing, spray
Water is aged 3h, and compression molding is cooled to room temperature then at 900 DEG C of calcining 20min in 250 DEG C of dryings, obtains cooling thing a, take cooling
Object a in mass ratio 3：1 is added deodorization matrix object to get biological deodorant.
Except ammonia bacterial strain screening culture medium：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：85 sequentially add beef
Cream peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening successively
Culture medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C sterilizing 20min to get.
Vulcanisation hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：5, which sequentially add beef extract-peptone, consolidates
Body culture medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening culture medium, unwrapping wire successively
Bacterium screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min.
Except ammine selective culture medium：According to the mass fraction, the ammonium hydroxide, 2 parts that 45 portions of sucrose, 13 parts of mass fractions are 25% are taken
KH2PO4, 0.5 part of MgSO4·7H2O, 0.2 part of FeSO4 , 0.002 part of ZnSO4 , 2 parts of NaCl, 1000 parts of water, 121 DEG C of sterilizings
20min。
Vulcanisation hydrogen selective medium：According to the mass fraction, 5 parts of glucose, 0.5 part of K are taken2HPO4, 2 parts of KNO3、0.5
Part MgCl2, 0.5 part of NaCl, 0.5 part of NH4Cl, 2 parts of Na2CO3, 0.02 part of FeCl2, 1000 parts of water, pH is naturally, 121 DEG C of sterilizings 15
min。
Assist microbial inoculum：According to the mass fraction, 3 parts of streptomyces microflavus, 4 parts of bacillus subtilises, 2 parts of Mucor circinelloides, 5 parts of glue are taken
Freeze sample bacillus, 7 parts of double qi Bacillus acidi lacticis, 7 parts of cloth Laplace saccharomycete.
A kind of preparation method of biological deodorant, includes the following steps：
（1）According to the mass fraction, 9 parts of fowl and livestock farm soil, 5 parts of activated sludge, 7 parts of landfill leachates, 4 parts of corrupt stalks are taken
Mixing must mix screening raw material, take mixing screening raw material in mass ratio 1：9 are added physiological saline, are diluted to 10-6Dilution grade,
Dilution is obtained, dilution is taken to be coated on successively except ammonia bacterial strain screening culture medium, 31 DEG C is corresponded respectively to and cultivates 2 days, 25 DEG C of cultures 4
It, 29 DEG C cultivate 2 days, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as J-1, J-2, J-3, take J-1, J-
2, in mass ratio 2 J-3：4：2 mixing, must mix and remove ammonia bacterium, take mixing except ammonia bacterium is seeded to by 4% inoculum concentration except ammine selective
It in culture medium, is cultivated 4 days in 27 DEG C, 180r/min, centrifugation takes pellet frozen to dry, obtains freeze-drying object；
（2）It takes dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, corresponds respectively to 31 DEG C and cultivate 2 days, 25 DEG C of trainings
Support 4 days, 29 DEG C cultivate 2 days, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as S-1, S-2, S-3, take S-1,
S-2, S-3 in mass ratio 3：3：4 mixing, must mix vulcanisation hydrogen bacterium, and the vulcanisation hydrogen bacterium of mixing is taken to be seeded to by 4% inoculum concentration
It in vulcanisation hydrogen selective medium, is cultivated 4 days in 27 DEG C, 180r/min, centrifugation takes pellet frozen to dry, must be freeze-dried
Object a takes freeze-drying object in mass ratio 2：4 are added a mixing of freeze-drying object, obtain deodorant base material；
（3）According to the mass fraction, take 7 portions of trehaloses, 13 portions of skimmed milks, 3 parts of starch Sodium Octenyl Succinates, 5 parts of beta-cyclodextrins,
6 parts of glycerine, 0.5 part of vitamin C, 0.6 part of xanthans, 9 portions of mannitol, 9 parts of lactose mixing, obtain mixture, take deodorant matrix
Object in mass ratio 6：4：13 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1150 DEG C of heat preservation 1h, is cooled to room temperature, obtains cooling thing, take aggregate diatom
Soil in mass ratio 6：7 are added cooling thing, add the zirconia ball of 2 times of cooling thing quality, ball milling 25min dry, pulverize
100 mesh sieve, and collect sieving particle, are sent out by quality, take 3 parts of paraffin, 3 parts of clays, 2 parts of sawdusts, 25 parts of sieving particulate matter mixing, spray
Water is aged 3h, and compression molding is cooled to room temperature then at 950 DEG C of calcining 25min in 275 DEG C of dryings, obtains cooling thing a, take cooling
Object a in mass ratio 4：2 are added deodorization matrix object to get biological deodorant.
Except ammonia bacterial strain screening culture medium：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：90 sequentially add beef
Cream peptone solid medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening successively
Culture medium, actinomyces screening and culturing medium, fungi screening culture medium, 121 DEG C sterilizing 20min to get.
Vulcanisation hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：6, which sequentially add beef extract-peptone, consolidates
Body culture medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening culture medium, unwrapping wire successively
Bacterium screening and culturing medium, fungi screening culture medium, 121 DEG C of sterilizing 20min.
Except ammine selective culture medium：According to the mass fraction, the ammonium hydroxide, 3 parts that 50 portions of sucrose, 15 parts of mass fractions are 25% are taken
KH2PO4, 0.7 part of MgSO4·7H2O, 0.3 part of FeSO4 , 0.003 part of ZnSO4 , 3 parts of NaCl, 1000 parts of water, 121 DEG C of sterilizings
20min。
Vulcanisation hydrogen selective medium：According to the mass fraction, 6 parts of glucose, 0.6 part of K is taken2HPO4, 3 parts of KNO3, 0.7 part
MgCl2, 0.6 part of NaCl, 0.6 part of NH4Cl, 3 parts of Na2CO3, 0.03 part of FeCl2, 1000 parts of water, pH is naturally, 121 DEG C of sterilizings 15
min。
Assist microbial inoculum：According to the mass fraction, 4 parts of streptomyces microflavus, 6 parts of bacillus subtilises, 3 parts of Mucor circinelloides, 7 parts of glue are taken
Freeze sample bacillus, 9 parts of double qi Bacillus acidi lacticis, 8 parts of cloth Laplace saccharomycete.
A kind of preparation method of biological deodorant, includes the following steps：
（1）According to the mass fraction, 10 parts of fowl and livestock farm soil, 7 parts of activated sludge, 9 parts of landfill leachates, 5 parts of corrupt straw are taken
Stalk mixes, and must mix screening raw material, takes mixing screening raw material in mass ratio 1：10 are added physiological saline, are diluted to 10-6Dilution
Grade, obtains dilution, dilution is taken to be coated on successively except ammonia bacterial strain screening culture medium, corresponds respectively to 35 DEG C and cultivates 3 days, 28 DEG C of trainings
Support 5 days, 33 DEG C cultivate 3 days, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as J-1, J-2, J-3, take J-1,
J-2, J-3 in mass ratio 3：5：3 mixing, must mix and remove ammonia bacterium, take mixing to remove ammonia bacterium and are seeded to by 5% inoculum concentration except ammonia selects
Property culture medium in, in 30 DEG C, 180r/min cultivate 5 days, centrifugation, take pellet frozen to dry, obtain freeze-drying object；
（2）It takes dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, corresponds respectively to 35 DEG C and cultivate 3 days, 28 DEG C of trainings
Support 5 days, 33 DEG C cultivate 3 days, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as S-1, S-2, S-3, take S-1,
S-2, S-3 in mass ratio 5：4：6 mixing, must mix vulcanisation hydrogen bacterium, and the vulcanisation hydrogen bacterium of mixing is taken to be seeded to by 5% inoculum concentration
It in vulcanisation hydrogen selective medium, is cultivated 5 days in 30 DEG C, 180r/min, centrifugation takes pellet frozen to dry, must be freeze-dried
Object a takes freeze-drying object in mass ratio 4：5 are added a mixing of freeze-drying object, obtain deodorant base material；
（3）According to the mass fraction, take 8 portions of trehaloses, 15 portions of skimmed milks, 4 parts of starch Sodium Octenyl Succinates, 6 parts of beta-cyclodextrins,
7 parts of glycerine, 0.8 part of vitamin C, 0.7 part of xanthans, 10 portions of mannitol, 12 parts of lactose mixing, obtain mixture, take deodorant base
Body object in mass ratio 8：5：15 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1200 DEG C of heat preservation 2h, is cooled to room temperature, obtains cooling thing, take aggregate diatom
Soil in mass ratio 7：9 are added cooling thing, add the zirconia ball of 3 times of cooling thing quality, ball milling 30min dry, pulverize
100 mesh sieve, and collect sieving particle, are sent out by quality, take 4 parts of paraffin, 5 parts of clays, 3 parts of sawdusts, 30 parts of sieving particulate matter mixing, spray
Water is aged 4h, and compression molding is cooled to room temperature then at 1000 DEG C of calcining 30min in 300 DEG C of dryings, obtains cooling thing a, take cooling
Object a in mass ratio 5：3 are added deodorization matrix object to get biological deodorant.
Comparative example：The biological deodorant of Fuyang City company production.
It is tested respectively using the deodorant of above-described embodiment and comparative example, during bacterium solution is sprayed, deodorizing microorganism
It to be uniformly sprayed on and be accelerated the ripening on mushroom bran carrier made of powder fermentation.Specific test result such as table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Number of viable（Hundred million/gram） | 5.1 | 4.9 | 4.9 | 2.4~2.6 |
| Methyl indol removal rate（%） | 92 | 94 | 92 | 77~79 |
| NH3Removal rate（%） | 93 | 94 | 92 | 73~75 |
| Hydrogen sulfide removal rate（%） | 91 | 93 | 91 | 75~79 |
In summary, biological deodorant comprehensive performance of the invention is better than presently commercially available product, and be worth promoting use.
Claims (7)
1. a kind of preparation method of biological deodorant, which is characterized in that the preparation method includes the following steps：
（1）According to the mass fraction, 8 ~ 10 parts of fowl and livestock farm soil, 3 ~ 7 parts of activated sludge, 6 ~ 9 parts of landfill leachates, 3 ~ 5 are taken
The corrupt stalk mixing of part, must mix screening raw material, mixing is taken to screen raw material in mass ratio 1：8 ~ 10 are added physiological saline, are diluted to
10-6Dilution grade, obtain dilution, dilution taken to be coated on successively except ammonia bacterial strain screening culture medium, correspond respectively to 28 ~ 35 DEG C training
Support, 23 ~ 28 DEG C of cultures, 25 ~ 33 DEG C of cultures, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, be named as J-1, J-2,
J-3 takes J-1, J-2, J-3 in mass ratio 1 ~ 3：2~5：1 ~ 3 mixing, must mix and remove ammonia bacterium, take mixing to remove ammonia bacterium and connect by 3 ~ 5%
Kind amount is seeded to except centrifugation is cultivated in ammine selective culture medium, is taken pellet frozen to dry, is obtained freeze-drying object；
（2）Take dilution to be coated on vulcanisation hydrogen bacterial strain screening culture medium successively, correspond respectively to 28 ~ 35 DEG C culture, 23 ~ 28 DEG C
Culture, 25 ~ 33 DEG C of cultures, three kinds of cultures of picking respectively obtain the maximum bacterium colony of bacterium diameter, are named as S-1, S-2, S-3, take S-1,
S-2, S-3 in mass ratio 2 ~ 5：1~4：3 ~ 6 mixing, must mix vulcanisation hydrogen bacterium, take and mix vulcanisation hydrogen bacterium by 3 ~ 5% inoculation
Amount, which is seeded in vulcanisation hydrogen selective medium, cultivates, and centrifuges, takes pellet frozen to dry, must be freeze-dried object a, takes freezing dry
Dry object in mass ratio 1 ~ 4：3 ~ 5 are added a mixing of freeze-drying object, obtain deodorant base material；
（3）According to the mass fraction, take 5 ~ 8 portions of trehaloses, 10 ~ 15 portions of skimmed milks, 2 ~ 4 parts of starch Sodium Octenyl Succinates, 4 ~ 6 parts
Beta-cyclodextrin, 4 ~ 7 parts of glycerine, 0.3 ~ 0.8 part of vitamin C, 0.4 ~ 0.7 part of xanthans, 8 ~ 10 portions of mannitol, 7 ~ 12 parts of lactose are mixed
It closes, obtains mixture, take deodorant matrix object in mass ratio 5 ~ 8：3~5：10 ~ 15 are added auxiliary microbial inoculum, mixture mixing, obtain deodorization
Matrix object；
（4）It takes diatomite to be placed in resistance furnace, is warming up to 1100 ~ 1200 DEG C of heat preservations, is cooled to room temperature, obtains cooling thing, take aggregate
Diatomite in mass ratio 5 ~ 7：6 ~ 9 are added cooling thing, add the zirconia ball of 2 ~ 3 times of cooling thing quality, ball milling is dry, powder
Broken sieving is collected sieving particle, is sent out by quality, and 1 ~ 4 part of paraffin, 2 ~ 5 parts of clays, 1 ~ 3 part of sawdust, 20 ~ 30 parts of sieving particles are taken
Object mixes, water spray 3 ~ 4h of ageing, compression molding, and calcining is cooled to room temperature, obtains cooling thing a, takes cooling thing a in mass ratio 3 ~ 5：1
~ 3 are added deodorization matrix object to get biological deodorant.
2. the preparation method of biological deodorant according to claim 1, which is characterized in that the step（1）In remove ammonia bacterium
Strain screening and culturing medium be：Take the ammonium hydroxide that mass fraction is 25% by Quality Mgmt Dept 1 respectively：80 ~ 90, which sequentially add beef extract-peptone, consolidates
Body culture medium, No. I solid medium of Gao Shi, Martin's formula solid medium, obtain corresponding bacteria screening culture medium, unwrapping wire successively
Bacterium screening and culturing medium, fungi screening culture medium, 121 DEG C sterilizing 20min to get.
3. the preparation method of biological deodorant according to claim 1, which is characterized in that the step（1）In except ammonia select
Selecting property culture medium：Except ammine selective culture medium：According to the mass fraction, it is 25% to take 40 ~ 50 portions of sucrose, 10 ~ 15 parts of mass fractions
Ammonium hydroxide, 1 ~ 3 part of KH2PO4, 0.3 ~ 0.7 part of MgSO4·7H2O, 0.1 ~ 0.3 part of FeSO4 , 0.001 ~ 0.003 part of ZnSO4 , 1 ~ 3 part
NaCl, 1000 parts of water, 121 DEG C of sterilizing 20min.
4. the preparation method of biological deodorant according to claim 1, which is characterized in that the step（2）In it is vulcanisation
Hydrogen bacterial strain screening culture medium：Take hydrogen sulfide by Quality Mgmt Dept 1 respectively：4 ~ 6 sequentially add beef extract-peptone solid medium, Gao Shi
No. I solid medium, Martin's formula solid medium, obtain successively corresponding bacteria screening culture medium, actinomyces screening and culturing medium,
Fungi screening culture medium, 121 DEG C of sterilizing 20min.
5. cadmium soil remediation biological agent according to claim 4, which is characterized in that the step（2）In vulcanisation hydrogen
Selective medium：According to the mass fraction, 3 ~ 6 parts of glucose, 0.3 ~ 0.6 part of K are taken2HPO4, 1 ~ 3 part of KNO3, 0.3 ~ 0.7 part
MgCl2, 0.3 ~ 0.6 part of NaCl, 0.3 ~ 0.6 part of NH4Cl, 1 ~ 3 part of Na2CO3, 0.01 ~ 0.03 part of FeCl2, 1000 parts of water, pH from
So, 121 DEG C of 15 min of sterilizing.
6. the preparation method of biological deodorant according to claim 1, which is characterized in that the step（3）Middle auxiliary bacterium
Agent：According to the mass fraction, 1 ~ 4 part of streptomyces microflavus, 2 ~ 6 parts of bacillus subtilises, 1 ~ 3 part of Mucor circinelloides, 4 ~ 7 portions of jelly are taken
Sample bacillus, 6 ~ 9 parts of double qi Bacillus acidi lacticis, 6 ~ 8 parts of cloth Laplace saccharomycete.
7. the preparation method of biological deodorant according to claim 1, which is characterized in that the step（4）Middle cooling thing a
Calcination condition be：In 250 ~ 300 DEG C of dryings, then at 900 ~ 1000 DEG C of 20 ~ 30min of calcining.
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| CN110893318A (en) * | 2019-12-07 | 2020-03-20 | 湖南科美洁环保科技有限公司 | Preparation method and use method of public toilet microbial deodorant |
| CN111758649A (en) * | 2019-03-26 | 2020-10-13 | 曹阳 | Coating stabilizer system for aquaculture pond bottom environment maintenance and preparation method thereof |
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| CN114053833A (en) * | 2020-08-03 | 2022-02-18 | 中国石油化工股份有限公司 | Blocking agent and application thereof, and method for blocking volatilization of volatile organic compounds and/or absorbing volatile organic compounds |
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