CN108739059A - A kind of pocket mushrooms culture substrate - Google Patents
A kind of pocket mushrooms culture substrate Download PDFInfo
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- CN108739059A CN108739059A CN201810468230.9A CN201810468230A CN108739059A CN 108739059 A CN108739059 A CN 108739059A CN 201810468230 A CN201810468230 A CN 201810468230A CN 108739059 A CN108739059 A CN 108739059A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The present invention relates to pocket mushrooms technical field of cultivation, and in particular to a kind of pocket mushrooms culture substrate.The pocket mushroom culture medium includes following raw material：40~75 parts of sugarcane, 15~33 parts of walnut shell, 12~25 parts of Chinese chestnut ball top, 13~23 parts of soybean stalks, 6~17 parts of rice bran, 1~5 part of potassium dihydrogen phosphate, 1~5 part of calcium sulfate, it is made by sugarcane processing, walnut shell processing, the processing of Chinese chestnut ball top, soybean stalks processing, mixed fermentation, charging 6 processing technologys of sterilization, the culture medium prepared is cylindrical, cylinder both ends can all be inoculated with plantation pocket mushrooms, effectively increase the utilization rate of culture substrate, improve pocket mushrooms yield, space in the canopy that matrix occupies is reduced, space availability ratio in canopy is improved；300~350 pin holes are drilled with per square meter on the plastic film of pocket mushrooms culture substrate provided by the invention surface layer simultaneously, effectively increase the ventilation effect of matrix and promote matrix moisturizing, ensure that Medium Culture moisture distribution is uniform.
Description
Technical field
The present invention relates to pocket mushrooms technical field of cultivation, and in particular to a kind of pocket mushrooms culture substrate.
Background technology
Pocket mushrooms, alias small mushroom, pleurotus cornucopiae are under the jurisdiction of Eumycota, Basidiomycetes, Agaricales, Pleurotaceae, Pleurotus.Sleeve
Precious mushroom belongs to nutrition height, and the low healthy food of heat is micro- containing protein, sugar part, fat, vitamin and iron, calcium etc.,
The content of its protein is higher than general vegetables.The amino acid contained type very abundant of pocket mushrooms, wherein containing necessary to human body
Eight kinds of amino acid, long-term consumption have the function of reducing hypertension and reduce cholesterol level.
Pocket mushrooms mushroom lid is in canescence, and lamella stem is white, and stem is mostly wilfully, to be bordering on middle life less.Fructification is mostly
It grows thickly, is less than Dan Sheng.7~30 DEG C of pocket mushrooms mycelial growth temperature, 23~26 DEG C of optimum temperature；Sporophore growth temperature 8~28
DEG C, 12~20 DEG C of optimum temperature.Its fruiting stage needs certain thermal stimulation to keep fruiting neat to accelerate fruiting body differentiation, produces
Amount increases.The water content of vegetative stage compost is advisable for 65~70%, relative air humidity 65%.The fruiting stage is empty
Gas relative humidity should be turned up to 85~95%, expect planted agent's moisturizing after adopting mushroom, if being air-dried partially dry with compost, mushroom body becomes smaller,
It can also cause former base atrophy, mushroom flower bud dead when serious.Elegant precious mushroom is aerobic fungi, whenever should all fully ensure that ventilation
Ventilative environment is allowed to keep good ecological state.Requirement of the vegetative stage to light be not stringent, in completely black dark environment
Under can also grow；The fructification stage is especially sensitive to illumination requirement, and in the case of unglazed, fructification is difficult to be formed, but direct light pair
It is unfavorable to form fructification, scattering light is advantageous to growing, and especially needs stronger scattering light in winter, keeps mushroom body partially white.
Existing pocket mushrooms implantation methods mostly use greatly packed plantation, and when plantation only selects the one side drilling of culture substrate to connect
The pocket mushroom strains of kind, largely waste culture substrate；It needs to pull open polybag when culture substrate moisturizing simultaneously, process complex operations are not
Just, and in compost moisture distribution is uneven.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of pocket mushrooms culture substrate, pass through the film in stromal surface
On be drilled with a large amount of apertures, facilitate culture substrate moisturizing, and ensure that Medium Culture moisture distribution is uniform.
The present invention is achieved by the following technical solutions：
A kind of pocket mushrooms culture substrate, including following raw material：
40~75 parts of sugarcane, 15~33 parts of walnut shell, 12~25 parts of Chinese chestnut ball top, 13~23 parts of soybean stalks, rice bran 6~
17 parts, 1~5 part of potassium dihydrogen phosphate, 1~5 part of calcium sulfate.
Preferably, including following raw material：
45~57 parts of sugarcane, 15~25 parts of walnut shell, 12~18 parts of Chinese chestnut ball top, 13~17 parts of soybean stalks, rice bran 7~
13 parts, 1~3 part of potassium dihydrogen phosphate, 1~3 part of calcium sulfate.
Preferably, including following raw material：
48 parts of sugarcane, 17 parts of walnut shell, 13 parts of Chinese chestnut ball top, 13 parts of soybean stalks, 7 parts of rice bran, 1 part of potassium dihydrogen phosphate,
1 part of calcium sulfate.
Preferably, the culture substrate preparation method is as follows：
A, sugarcane is handled：Weigh the sugarcane of required parts by weight, even root cleans soil, peeling, sugarcane skin chopping for it is long by 0.5~
1cm, the strip of wide 0.2~0.3cm are spare；Sugar cane crushing, it is that 1~3mm powders are spare that bagasse, which crushes, and juice is spare；
B, walnut shell is handled：The walnut shell for weighing required parts by weight, crushes the powder for 1~3mm, powder and crushes out core
Peach shell oil is mixed evenly spare；
C, Chinese chestnut ball top is handled：The Chinese chestnut ball top for weighing required parts by weight is placed in rolling machine and rubs surface layer acne, then
It is 11~15% to be placed in dryer and be dried to ball top water content, is crushed spare for the powder of 1~3mm；
D, soybean stalks are handled：The soybean stalks for weighing required parts by weight, it is 11~13% to be dried to water content, shreds and is
The powder of 0.5~1cm long is spare；
E, mixed fermentation：The spare sugarcane skin, standby after slitting is sequentially added in the blender of 135~150r/min of rotating speed
With soybean stalks powder, spare walnut shell powder, standby plate hairy chestnut cup powder, spare bagasse, rice bran, potassium dihydrogen phosphate, sulphur
Sour calcium adds another raw material after a kind of raw material stirring 5min is often added；Raw material continues to stir 10min after adding, then adds
It is 68~70% to enter zymotic fluid to mixture moisture content, is transferred to seal in fermentation tank by moistening mixture after stirring 35min and send out
3~5d of ferment；
F, charging sterilization：It waits for opening fermentation tank after fermentation, divulge information 3~5h, and fermentation material, which is transferred to thickness, is
In the sealed polyethylene plastic of 0.22mm, every film feeds 7~10kg, and film rolls extruding, until to cling plastics thin for fermentation material
Film, cylindrical, 35~50cm of column length, 8~10cm of diameter, two centriciput of cylinder are respectively drilled with deep 2~3cm, 1~3cm of diameter
Aperture tightens cylinder both ends plastic film, and cord binding in cylinder middle part tightens, ultraviolet-sterilization after high temperature sterilization, sterilization knot
Hole is pricked after beam on a plastic film, 300~350,0.3~0.5mm of aperture, hole, finished product pocket mushrooms are pricked on every square meter film
Culture substrate is spare.
Preferably, the zymotic fluid includes following raw material：
500 times of sugar-cane juice, 95 parts of dilution, 0.5 part of dry ferment, 1 part of peptone, 1 part of mannitol, 1 part of glucose.
Preferably, the ferment tank temperature is 42~47 DEG C, the one time fermentation liquid of sealing stirring for 24 hours 9 that often ferments~
10min, breathe freely 1~1.5min.
Preferably, the pocket mushrooms culture substrate high temperature sterilization temperature is 130~135 DEG C, waits for that substrate temperature is reduced to 60
30~35min of ultraviolet-sterilization after~65 DEG C.
In technical solution provided by the invention, the pocket mushrooms culture substrate inoculation method is as follows：
Cylindric both ends film is wound off, 1~3cm of compost is leaked out, sprays water to culture substrate to culture amount across film
Moisture is 68~70%, and moisture distribution is uniform in compost；
" pocket No. 2 " strain is inoculated under gnotobasis in the both ends aperture of cylindric culture substrate, routinely manager
Method Cultivate administration pocket mushrooms.
The beneficial effects of the invention are as follows：
1, the present invention selects sugarcane to prepare pocket mushrooms culture substrate as primary raw material, each position of sugarcane is added
Enter the preparation process to culture substrate, effectively increase the utilization rate of primary raw material, reduces the wasting of resources, reduce making for other raw materials
Dosage；
2, the cylindric culture substrate that the present invention prepares can be inoculated with plantation pocket mushrooms at cylinder both ends, increase matrix and utilize
Rate improves pocket mushrooms yield, reduces space in the canopy that matrix occupies, and improves space availability ratio in canopy；
3,300~350 pin holes are drilled with per square meter on the plastic film of pocket mushrooms culture substrate provided by the invention surface layer, had
Effect increases the ventilation effect of matrix and promotes matrix moisturizing, ensures that Medium Culture moisture distribution is uniform.
Specific implementation mode
The present invention is further described in detail With reference to embodiment, but technical solution provided by the invention
It include not only the content showed in embodiment.
Embodiment 1
Present embodiments provide a kind of pocket mushrooms culture substrate disclosed by the invention and preparation method thereof, select the sugarcane to be
Primary raw material, detailed process are as follows：
Step 1: preparing following raw material：
Sugarcane 480kg, walnut shell 170kg, Chinese chestnut ball top 130kg, soybean stalks 130kg, rice bran 70kg, potassium dihydrogen phosphate
10kg, calcium sulfate 10kg；
Step 2: sugarcane is handled：The sugarcane of required parts by weight is weighed, even root cleans soil, peeling, and sugarcane skin chopping is length
0.5cm, the strip of width 0.2cm are spare；Sugar cane crushing, it is that 1mm powders are spare that bagasse, which crushes, and juice is spare；
Step 3: prepared by zymotic fluid：Weigh 500 times of dilution 95kg of sugar-cane juice, be added dry ferment 500g, peptone 1kg,
Mannitol 1kg, glucose 1kg, are mixed evenly, and are filtered after standing 3h, take filtrate to make zymotic fluid spare；
Step 4: walnut shell is handled：The walnut shell for weighing required parts by weight, crushes the powder for 1mm, powder with crush out
Walnut shell oil is mixed evenly spare；
Step 5: Chinese chestnut ball top is handled：The Chinese chestnut ball top for weighing required parts by weight, is placed in rolling machine and rubs surface layer powder
Thorn, then be placed in dryer be dried to ball top water content be 11%, crush spare for the powder of 1mm；
Step 6: soybean stalks are handled：The soybean stalks for weighing required parts by weight, it is 11% to be dried to water content, chopping
It is spare for the powder of 0.5cm long；
Step 7: mixed fermentation：The spare sugarcane skin, standby after slitting is sequentially added in the blender of rotating speed 150r/min
With soybean stalks powder, spare walnut shell powder, standby plate hairy chestnut cup powder, spare bagasse, rice bran, potassium dihydrogen phosphate, sulphur
Sour calcium adds another raw material after a kind of raw material stirring 5min is often added；Raw material continues to stir 10min after adding, then adds
It is 70% to enter zymotic fluid to mixture moisture content, and moistening mixture is transferred in fermentation tank after stirring 35min and is sealed by fermentation 3d；
Fermentation temperature is 45 DEG C, and often ferment the one time fermentation liquid 10min of sealing stirring for 24 hours, and breathe freely 1min；
Step 8: charging sterilization：It waits for opening fermentation tank after fermentation, divulge information 5h, and fermentation material, which is transferred to thickness, is
In the sealed polyethylene plastic of 0.22mm, every film charging 9kg, film rolls extruding, until fermentation material clings plastic film, is in
Cylindrical shape, column length 45cm, diameter 10cm, two centriciput of cylinder are respectively drilled with the aperture of deep 3cm, diameter 2cm, by cylinder both ends plastics
Film tightens, and cord binding in cylinder middle part tightens, and sterilizes 15min in 135 DEG C of hot air type baking oven high temperatures, waits for substrate temperature
Ultraviolet-sterilization 35min, pricks hole on a plastic film after being reduced to 60 DEG C after sterilization, and 350, hole, hole are pricked on every square meter film
Diameter 0.5mm, finished product pocket mushrooms culture substrate.
In the present embodiment, always ferment zymotic fluid about 1121.6kg, prepares cylindric culture substrate about 123.
Embodiment 2
Present embodiments provide a kind of pocket mushrooms culture substrate disclosed by the invention and preparation method thereof, select the sugarcane to be
Primary raw material, detailed process are as follows：
Step 1: preparing following raw material：
Sugarcane 520kg, walnut shell 150kg, Chinese chestnut ball top 120kg, 130kg parts of soybean stalks, rice bran 80kg, biphosphate
Potassium 10kg, calcium sulfate 10kg；
Step 2: sugarcane is handled：The sugarcane of required parts by weight is weighed, even root cleans soil, peeling, and sugarcane skin chopping is length
0.7cm, the strip of width 0.3cm are spare；Sugar cane crushing, it is that 2mm powders are spare that bagasse, which crushes, and juice is spare；
Step 3: prepared by zymotic fluid：Weigh 500 times of dilution 95kg of sugar-cane juice, be added dry ferment 500g, peptone 1kg,
Mannitol 1kg, glucose 1kg, are mixed evenly, and are filtered after standing 2h, take filtrate to make zymotic fluid spare；
Step 4: walnut shell is handled：The walnut shell for weighing required parts by weight, crushes the powder for 2mm, powder with crush out
Walnut shell oil is mixed evenly spare；
Step 5: Chinese chestnut ball top is handled：The Chinese chestnut ball top for weighing required parts by weight, is placed in rolling machine and rubs surface layer powder
Thorn, then be placed in dryer be dried to ball top water content be 15%, crush spare for the powder of 2mm；
Step 6: soybean stalks are handled：The soybean stalks for weighing required parts by weight, it is 13% to be dried to water content, chopping
It is spare for the powder of 0.7cm long；
Step 7: mixed fermentation：The spare sugarcane skin, standby after slitting is sequentially added in the blender of rotating speed 140r/min
With soybean stalks powder, spare walnut shell powder, standby plate hairy chestnut cup powder, spare bagasse, rice bran, potassium dihydrogen phosphate, sulphur
Sour calcium adds another raw material after a kind of raw material stirring 5min is often added；Raw material continues to stir 10min after adding, then adds
It is 68% to enter zymotic fluid to mixture moisture content, and moistening mixture is transferred in fermentation tank after stirring 35min and is sealed by fermentation 4d；
Fermentation temperature is 42 DEG C, and often ferment the one time fermentation liquid 10min of sealing stirring for 24 hours, and breathe freely 1.5min；
Step 8: charging sterilization：It waits for opening fermentation tank after fermentation, divulge information 5h, and fermentation material, which is transferred to thickness, is
In the sealed polyethylene plastic of 0.22mm, every film charging 10kg, film rolls extruding, until fermentation material clings plastic film,
Cylindrical, column length 50cm, diameter 10cm, two centriciput of cylinder are respectively drilled with the aperture of deep 3cm, diameter 2cm, and cylinder both ends are moulded
Material film tightens, and cord binding in cylinder middle part tightens, and sterilizes 25min in 130 DEG C of hot air type baking oven high temperatures, waits for matrix temperature
Ultraviolet-sterilization 30min after degree is reduced to 60 DEG C, pricks hole on a plastic film after sterilization, 300, hole is pricked on every square meter film,
Aperture 0.5mm, finished product pocket mushrooms culture substrate.
In the present embodiment, always ferment zymotic fluid about 1108.9kg, prepares cylindric culture substrate about 122.
Experiment 1
In this experimental group, the culture medium of 20 embodiments 1 preparation is selected at random, culture medium prepared by 20 embodiments 2,
20 bags of the packed pocket mushroom culture medium of the routine peddled in the market is randomly selected again, and specific experimental method is as follows：
A groups：Culture medium both ends film prepared by 20 embodiments 1 that will be singled out is wound off, and leaks out compost 2cm, across
It is 68% that film, which is sprayed water to culture substrate to culture amount moisture,；In the both ends aperture of cylindric culture substrate under gnotobasis
Middle inoculation " pocket No. 2 " strain, every is inoculated with 1kg, routinely management method Cultivate administration pocket mushrooms；
B groups：Culture medium both ends film prepared by 20 embodiments 2 that will be singled out is wound off, and leaks out compost 2cm, across
It is 68% that film, which is sprayed water to culture substrate to culture amount moisture,；In the both ends aperture of cylindric culture substrate under gnotobasis
Middle inoculation " pocket No. 2 " strain, every is inoculated with 1kg, routinely management method Cultivate administration pocket mushrooms；
C groups：The packed pocket mushrooms culture medium sack of routine that randomly select 20 bags are peddled in the market is opened, to culture medium
It is 68% that matter, which is sprayed water to culture amount moisture, in inoculation " pocket No. 2 " pocket mushroom strains 1kg under gnotobasis, is routinely managed
Reason method Cultivate administration pocket mushrooms.
In this experiment, it is directly to spray water to media surface that A, which combines B group culture medium method for supplementing water,；C group culture medium moisturizings
Method is to pull open polybag, and into bag, culture medium is sprayed water.
In this experiment, the moisture at each position in A groups, B groups, C groups after moisturizing is detected, the results are shown in Table 1.
| Each position moisture | Culture medium surface layer | In the middle part of culture medium | Cultivate basal layer |
| A groups | 68% | 67.3% | 67.8% |
| B groups | 68% | 67.5% | 6.5% |
| C groups | 68% | 63.0% | 60.3% |
Table 1
As shown in Table 1, for conventional packed pocket mushroom culture medium, the pocket mushrooms of embodiment 1 and the offer of embodiment 2
Evenly, each position of culture medium is suitable for the cultivation of pocket mushrooms to moisture distribution to culture medium after moisturizing；
It is proved simultaneously by detecting, the pocket mushroom culture medium internal gas permeability that embodiment 1 and embodiment 2 provide is much
More than conventional packed pocket mushroom culture medium.
In this experiment, it is 70650cm that A groups culture medium stacks space in canopy3；B group culture mediums stack space in canopy
For 78500cm3；It is 83450cm that C group culture mediums stack space in canopy3。
In this experiment, tri- groups of culture mediums of A, B, C are inoculated with " pocket No. 2 " pocket mushroom strains 1kg, A groups and B group culture mediums
It respectively is inoculated with 0.5kg at both ends, C groups culture medium is inoculated with strain 1kg in culture medium upper surface, and identical training is pressed in identical greenhouse
Plant method cultivates pocket mushrooms.
The experimental results showed that：
Culture medium starts the damp pocket mushrooms of harvesting first in A groups after 3 months, and culture medium both ends grow pocket mushrooms, and pocket
Mushroom growing way is uniform, every culture medium harvest pocket mushrooms 2.1kg；Culture medium starts the damp pocket mushrooms culture of harvesting first in B groups after March
Base both ends grow pocket mushrooms, and pocket mushrooms growing way is uniform, every culture medium harvest pocket mushrooms 2.0kg；C groups culture medium 3.5 months
After start the first damp pocket mushrooms of harvesting, for pocket mushrooms dense growth in packed culture medium upper surface, size distribution is uneven, every bag of culture
Base harvests pocket mushrooms 1.5kg.
Experiment 2
In this experimental group, the culture medium of 20 embodiments 1 preparation is selected at random, culture medium prepared by 20 embodiments 2,
20 bags of the packed pocket mushroom culture medium of the routine peddled in the market is randomly selected again, and specific experimental method is as follows：
D groups：Culture medium both ends film prepared by 20 embodiments 1 that will be singled out is wound off, and leaks out compost 2cm, across
It is 68% that film, which is sprayed water to culture substrate to culture amount moisture,；In the both ends aperture of cylindric culture substrate under gnotobasis
Middle inoculation " pocket No. 2 " strain, both ends are respectively inoculated with 1kg, routinely management method Cultivate administration pocket mushrooms；
E groups：Culture medium both ends film prepared by 20 embodiments 2 that will be singled out is wound off, and leaks out compost 2cm, across
It is 68% that film, which is sprayed water to culture substrate to culture amount moisture,；In the both ends aperture of cylindric culture substrate under gnotobasis
Middle inoculation " pocket No. 2 " strain, both ends are respectively inoculated with 1kg, routinely management method Cultivate administration pocket mushrooms；
F groups：The packed pocket mushrooms culture medium sack of routine that randomly select 20 bags are peddled in the market is opened, to culture medium
It is 68% that matter, which is sprayed water to culture amount moisture, in inoculation " pocket No. 2 " pocket mushroom strains 1kg under gnotobasis, is routinely managed
Reason method Cultivate administration pocket mushrooms.
The experimental results showed that：
Culture medium starts the damp pocket mushrooms of harvesting first in A groups after 3 months, and culture medium both ends grow pocket mushrooms；It is trained in B groups
Start the damp pocket mushrooms of harvesting first after foster base March, culture medium both ends grow pocket mushrooms；C groups culture medium starts to harvest after 3.5 months
First damp pocket mushrooms, pocket mushrooms dense growth is in packed culture medium upper surface；
Meanwhile being found by observation and calculating, in identical canopy in space, pocket mushroom culture medium phase provided by the invention
For conventional pocket mushroom culture medium, double ripe pocket mushrooms, and pocket mushrooms growing way and battalion can be but harvested in identical space
Foster value is not less than the pocket mushrooms that conventional medium cultivates.
The above described is only a preferred embodiment of the present invention, being not intended to limit the present invention in any form, appoint
What without departing from technical solution of the present invention content, according to the technical essence of the invention to made by above example it is any modification, etc.
With variation and modification, in the range of still falling within technical solution of the present invention.
Claims (7)
1. a kind of pocket mushrooms culture substrate, which is characterized in that including following raw material：
40~75 parts of sugarcane, 15~33 parts of walnut shell, 12~25 parts of Chinese chestnut ball top, 13~23 parts of soybean stalks, rice bran 6~17
Part, 1~5 part of potassium dihydrogen phosphate, 1~5 part of calcium sulfate.
2. a kind of pocket mushrooms culture substrate according to claim 1, which is characterized in that including following raw material：
45~57 parts of sugarcane, 15~25 parts of walnut shell, 12~18 parts of Chinese chestnut ball top, 13~17 parts of soybean stalks, rice bran 7~13
Part, 1~3 part of potassium dihydrogen phosphate, 1~3 part of calcium sulfate.
3. a kind of pocket mushrooms culture substrate according to claim 1, which is characterized in that including following raw material：
48 parts of sugarcane, 17 parts of walnut shell, 13 parts of Chinese chestnut ball top, 13 parts of soybean stalks, 7 parts of rice bran, 1 part of potassium dihydrogen phosphate, sulfuric acid
1 part of calcium.
4. a kind of pocket mushrooms culture substrate according to claims 1 to 3 any one, which is characterized in that the culture medium
Matter preparation method is as follows：
A, sugarcane is handled：Weigh the sugarcane of required parts by weight, even root cleans soil, peeling, sugarcane skin chopping is long 0.5~1cm,
The strip of wide 0.2~0.3cm is spare；Sugar cane crushing, it is that 1~3mm powders are spare that bagasse, which crushes, and juice is spare；
B, walnut shell is handled：The walnut shell for weighing required parts by weight, crushes the powder for 1~3mm, powder and crushes out walnut shell
Oil is mixed evenly spare；
C, Chinese chestnut ball top is handled：The Chinese chestnut ball top for weighing required parts by weight, is placed in rolling machine and rubs surface layer acne, then be placed in
It is 11~15% that ball top water content is dried in dryer, is crushed spare for the powder of 1~3mm；
D, soybean stalks are handled：The soybean stalks for weighing required parts by weight, it is 11~13% to be dried to water content, and it is 0.5 to shred
The powder of~1cm long is spare；
E, mixed fermentation：The spare sugarcane skin after slitting, spare Huang are sequentially added in the blender of 135~150r/min of rotating speed
Beanstalk stalk powder, spare walnut shell powder, standby plate hairy chestnut cup powder, spare bagasse, rice bran, potassium dihydrogen phosphate, calcium sulfate,
Another raw material is added after a kind of raw material stirring 5min is often added；Raw material continues to stir 10min after adding, and adds fermentation
Liquid to mixture moisture content be 68~70%, stir 35min after will moistening mixture be transferred in fermentation tank be sealed by fermentation 3~
5d；
F, charging sterilization：It waits for opening fermentation tank after fermentation, divulge information 3~5h, and it is 0.22mm's that fermentation material, which is transferred to thickness,
In sealed polyethylene plastic, every film 7~10kg of charging, film rolls extruding, until fermentation material clings plastic film, it is in cylinder
Shape, 35~50cm of column length, 8~10cm of diameter, two centriciput of cylinder are respectively drilled with the aperture of deep 2~3cm, 1~3cm of diameter, will justify
Cylinder both ends plastic film tightens, and cord binding in cylinder middle part tightens, ultraviolet-sterilization after high temperature sterilization, in plastics after sterilization
Hole is pricked on film, pricks 300~350,0.3~0.5mm of aperture, hole on every square meter film, finished product pocket mushrooms culture substrate is standby
With.
5. a kind of pocket mushrooms culture substrate according to claim 4, which is characterized in that the zymotic fluid includes following weight
Part raw material：
500 times of sugar-cane juice, 95 parts of dilution, 2 parts of dry ferment, 1 part of peptone, 1 part of mannitol, 1 part of glucose.
6. a kind of pocket mushrooms culture substrate according to claim 4, which is characterized in that the ferment tank temperature is 42
~47 DEG C, often ferment the one time fermentation 9~10min of liquid of sealing stirring for 24 hours, and breathe freely 1~1.5min.
7. a kind of pocket mushrooms culture substrate according to claim 4, which is characterized in that the pocket mushrooms culture substrate high temperature
Sterilization temperature is 130~135 DEG C, 30~35min of ultraviolet-sterilization after substrate temperature is reduced to 60~65 DEG C.
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| CN110669705A (en) * | 2019-11-19 | 2020-01-10 | 曲阜师范大学 | Lysinibacillus fusiformis CA1 and application thereof in promoting growth of pleurotus geesteranus |
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