CN108728261B

CN108728261B - Environment-friendly physical bacteriostatic lotion and application thereof

Info

Publication number: CN108728261B
Application number: CN201810463251.1A
Authority: CN
Inventors: 金龙
Original assignee: Bds Shanghai Health Technology Co ltd
Current assignee: Guangzhou Lanfeng daily necessities Co.,Ltd.
Priority date: 2018-05-15
Filing date: 2018-05-15
Publication date: 2020-09-08
Anticipated expiration: 2038-05-15
Also published as: CN108728261A

Abstract

The invention provides an environment-friendly physical bacteriostatic lotion which comprises the following components in percentage by weight: 4-8% of chitosan derivative, 15-25% of chamomile extract, 0.4-0.8% of dodecyl dimethyl amine oxide, 0.2-0.5% of coconut oil diethanolamide, a proper amount of 1% citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water. The environment-friendly physical bacteriostatic lotion has a strong bacteriostatic effect, plays a good bacteriostatic role on various strains, has a synergistic effect when being mixed with various raw materials, and is wide in application range and remarkable in effect, and particularly has four strains of candida albicans, staphylococcus aureus, escherichia coli and bacillus subtilis.

Description

Environment-friendly physical bacteriostatic lotion and application thereof

Technical Field

The invention belongs to the field of daily chemical sanitation, particularly belongs to the technical field of textile finishing agents, mainly relates to a laundry detergent, and particularly relates to an environment-friendly physical bacteriostatic lotion and application thereof.

Background

With the improvement of the living standard of people, the requirements of people on the quality of life are higher and higher, researches show that many skin diseases and gastrointestinal diseases of human bodies are mostly caused by the damage of internal microenvironment due to the invasion of harmful bacteria, textile clothes which are in closest contact with the skin and hands and feet cannot meet the effects of bacteriostasis and sterilization only by daily washing, but can form hotbeds for various bacteria breeding due to sweat stains, body fluids and milk stains, so that the skin is infected with bacteria to generate the phenomena of eczema, redness and swelling and the like, therefore, high-efficiency, low-toxicity, safety and convenience bacteriostatic products gradually become necessary products for the life of people, and washing powder replacing washing powder becomes necessary products in modern life. The existing market has various bacteriostatic products, the bacteriostatic effect and the related performance are different, the laundry detergent is generally divided into common laundry detergent and underwear laundry detergent, however, the laundry detergent on the market, no matter whether the common laundry detergent or the underwear laundry detergent is used as a simple cleaning agent in the washing process, only can achieve the decontamination effect, and does not have the efficient bacteriostatic, antibacterial and anti-mildew effects. In addition, the commercial laundry detergent is mostly compounded with a surfactant and some alkaline auxiliaries to achieve the purpose of decontamination, and although a neutral product exists, the antibacterial effect is difficult to achieve. Therefore, it is a research focus to obtain a good decontamination effect and effectively inhibit the growth of bacteria, mold and the like in a certain time and in a humid environment, so as to keep the activity of microorganisms such as bacteria, fungi, algae, yeasts, viruses and the like at a low level and inhibit the growth of the microorganisms.

Common antibacterial modes mainly include antibiotic antibacterial mode and common chemical toxicity antibacterial mode, although the antibacterial mode of the antibiotic has high sterilization speed and high efficiency, the antibiotic has high danger, toxic and side effects, anaphylactic reaction and double infection can be caused by improper use, bacterial drug resistance is increased to initiate super bacteria, super drug-resistant bacteria are generated, the super bacteria have resistance to common bactericidal drug antibiotics, once human is infected, the treatment of patients is difficult, and sometimes even the life is threatened. In the chemical antibacterial mode, the common chemical disinfectant generally contains chlorine and phenol components, and the sterilization principle is that the venom permeates into microbial cells through osmotic pressure to destroy protein components of the microbial cells, so that the aim of sterilization is fulfilled. After a period of use, the microorganisms are likely to develop some resistance to the disinfectant, which gradually leads to the formation of superbacteria. And the antibacterial process is usually accompanied with safety problems such as chemical corrosion, chemical residue and the like, the antibacterial agent is diluted in general use, and when the dilution concentration is lower than the osmotic pressure in the microorganism, toxic components cannot be injected into cells, so that the antibacterial effect is greatly reduced, and simultaneously, the antibacterial agent is accompanied with irritating unpleasant odor in the use process. As a brand new antibacterial mode, the physical antibacterial mode is different from antibiotic antibacterial and common chemical toxicity antibacterial modes, and the physical mode is used for inhibiting or killing bacteria. Bacteria are composed of cell walls, cell membranes, cytoplasm and nucleus, which are composed of phospholipid bilayers, and are negatively charged under neutral conditions. Negatively charged bacteria are attracted to cations (e.g., organic quaternary ammonium groups) on the antimicrobial material, which bind the bacteria's freedom of movement and inhibit their respiratory function, i.e., contact death occurs. In addition, under the action of the electric field, the negative charges on the cell wall and the cell membrane are unevenly distributed to cause deformation and physical rupture, so that the contents of the cell, such as water, protein and the like, are exuded out of the body, and the bacteria are dissolved to die. Through the mode of physical bacteriostasis, the environment of bacterial breeding and living is directly cut off, and the bacteria can not fall off and enter a human body like chemical drugs, so that the irritation, the anaphylaxis and the toxic and side effects can not exist, and because the bacterial epidermis is negative, the physical bacteriostasis and the sterilization are broad-spectrum, the immunity of people can not be reduced or drug-resistant strains can not be generated, and the effect of inhibiting bacteria safely, environmentally friendly and healthily can be achieved.

In view of this, the invention provides an environmentally-friendly physical bacteriostatic lotion with broad antibacterial performance, high antibacterial efficiency, lasting antibacterial effect and no toxicity, and the problem to be solved is urgent.

Disclosure of Invention

Therefore, the invention provides the natural environment-friendly physical bacteriostatic lotion which can be used for synergistic bacteriostasis and sterilization, is suitable for daily life, and has a wide bacteriostatic action range and no toxicity and harm.

Therefore, the invention provides an environment-friendly physical bacteriostatic lotion which comprises the following components in percentage by weight: 4-8% of chitosan derivative, 15-25% of chamomile extract, 0.4-0.8% of dodecyl dimethyl amine oxide, 0.2-0.5% of coconut oil diethanolamide, a proper amount of 1% citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water.

The environment-friendly physical bacteriostatic lotion preferably comprises, by weight, 6-8% of chitosan derivative, 15-20% of chamomile extract, 0.4-0.6% of dodecyl dimethyl amine oxide, 0.3-0.4% of coconut oil diethanolamide, a proper amount of 1% of citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water.

The environment-friendly physical bacteriostatic lotion preferably comprises, by weight, 6% of chitosan derivative, 20% of chamomile extract, 0.6% of lauryl dimethyl amine oxide, 0.3% of coconut diethanolamide, a proper amount of 1% of citric acid to adjust the pH to 6.0-7.0, and the balance of deionized water.

The environment-friendly physical bacteriostatic lotion preferably comprises, by weight, 8% of chitosan derivative, 15% of chamomile extract, 0.4% of lauryl dimethyl amine oxide, 0.4% of coconut diethanolamide, a proper amount of 1% of citric acid to adjust the pH to 6.0-7.0, and the balance of deionized water.

The chitosan referred to in the application is also called deacetylated chitin, has free amino groups in molecules, is easy to form salt in an acid solution, and has cationic property. The amino group characteristics of chitosan are more remarkable as the number of amino groups in the molecule increases. The chitosan derivative has the advantages that the chitosan derivative has protonated ammonium, the protonated ammonium can interact with a cell membrane with negative charges of bacteria, the function of the cell membrane of the bacteria is interfered, cytoplasm in a bacteria body is lost, and normal physiological metabolism of cells is disturbed, so that the aim of sterilization is fulfilled, the water solubility of the chitosan derivative is increased through modification reactions such as hydroxylation, carboxylation, acylation, quaternization and the like, the quaternized chitosan derivative is an ideal chitosan derivative with a good antibacterial effect, and in the aspect of antibacterial broad spectrum, the effect of the chitosan derivative needs to be improved. According to the application, a chitosan derivative with a very good antibacterial effect is synthesized by researching the chitosan derivative, the chitosan derivative has a good antibacterial effect on strains such as staphylococcus aureus and escherichia coli, but the effect on strains such as candida albicans and bacillus subtilis is not obvious, so that in order to make up for the problem that the antibacterial broad spectrum of the chitosan derivative is relatively weak, a chamomile extract is selected as an antibacterial component to be compounded with the chitosan derivative. The chamomile extract belongs to a natural product extracted by taking natural substances as main raw materials, has the characteristics of being mild, non-irritating and easy to absorb, and the active ingredient apigenin belongs to flavonoid substances, has the effects of resisting oxidation, resisting vascular proliferation, diminishing inflammation, resisting bacteria and the like, is strong in antibacterial property and antioxidant effect, does not need to be additionally added with a preservative when in use, can be used as a main active ingredient to play a role in inhibiting bacteria, and can be used for protecting a product, prolonging the shelf life of the product and coordinating and synergizing to promote the better play of all components through the strong antioxidant effect and high stability of the product.

The preparation method of the chitosan derivative comprises the following steps: dissolving N-methyl-N-dodecyl-N, N-dihydroxyethyl ammonium bromide by using a proper amount of chloroform, adding 4-dimethylaminopyridine and triethylamine, cooling in an ice-water bath, then dropwise adding p-methylbenzenesulfonyl chloride dissolved in the chloroform into the mixed solution, wherein the molar ratio of the N-methyl-N-dodecyl-N, N-dihydroxyethyl ammonium bromide to the p-methylbenzenesulfonyl chloride to the triethylamine to the 4-dimethylaminopyridine is 3:1:1.5:0.05, stirring at room temperature after ice-bath reaction until the reaction is complete, decompressing and steaming to remove the chloroform to obtain a white solid intermediate 1, and drying in vacuum for later use. Dissolving N-benzylidene-chitosan in isopropanol, adding NaOH solution, stirring, adding the intermediate 1 into the solution, heating to a certain temperature for reaction for enough time, cooling, adding absolute ethyl alcohol, performing suction filtration, adding acetone: washing the ethanol mixed solution for 3 times, drying in vacuum to obtain an intermediate 2, dissolving the intermediate 2 in 10mL of 0.25mol/L hydrochloric acid/ethanol solution, stirring at room temperature for reaction, performing suction filtration, washing with acetone for 3 times, drying, and purifying to obtain the chitosan derivative.

The preparation method of the chamomile extract comprises the following steps: pulverizing flos Matricariae Chamomillae, soaking in 5-20 times of water for 5-8 hr, performing ultrasonic treatment for 1 hr, filtering, soaking in 8-10 times of 40% ethanol solution, performing ultrasonic treatment for 30min, filtering, mixing the water soaking solution and ethanol soaking solution, performing steam distillation, extracting for 2-4 hr, and collecting volatile oil extract.

The coconut oil diethanolamide is a nonionic surfactant, the dodecyl dimethyl amine oxide is an amphoteric surfactant, is cationic in an acidic medium, is easy to dissolve in water, has the functions of thickening and stabilizing foam, is good in compatibility with the cationic surfactant, can fully play the functions of thickening, wetting and stabilizing foam when being applied to lotion, is very mild to clothes or skin, can effectively reduce the irritation of other components in the composition, and plays an important role in the coordination and synergism of the chitosan derivative and the chamomile extract due to the combined use of the chitosan derivative and the chamomile extract. Due to the interaction between the lauryl dimethyl amine oxide and the coconut oil diethanolamide, the surfactant can stably exist in a compound system, and when the dosage ratio of the lauryl dimethyl amine oxide to the coconut oil diethanolamide is 2: 1, the solubility is best, aggregation and sedimentation are not easy to occur, and the stability of the washing liquid is highest.

The invention also provides a preparation method of the environment-friendly physical bacteriostatic lotion, which comprises the steps of mixing the chitosan derivative prepared by the method with a chamomile extract according to a proportion, adding a certain amount of deionized water for dissolving, heating at 40-50 ℃ for 20-40 minutes, dissolving at the rotation speed of 800-1000r/min, adding coconut oil diethanolamide and dodecyl dimethyl amine oxide according to the proportion while stirring at the conditions of 26-28 ℃ and the rotation speed of 1200r/min, simultaneously adding a proper amount of 1% citric acid solution for regulating the pH value to be 6-7, preferably regulating the pH value to 6.8, ensuring that the stirring rotation speed is uniform in the process, fully mixing all the substances, standing and packaging to obtain the environment-friendly physical bacteriostatic lotion.

The invention also provides application of the environment-friendly physical bacteriostatic lotion in daily chemicals or washing products.

The daily chemical or washing product is a laundry detergent with antibacterial effect, and is used by taking 30-50ml of the product, adding 2-3kg of water, soaking clothes for 15-20 minutes, and washing normally.

The environment-friendly physical bacteriostatic lotion produced by the process has no essence and no irritant ingredient, is normal in system, has no turbidity and precipitation phenomena after long-term shelf storage test (3 years at normal temperature) and repeated heat resistance (40 +/-2 ℃ and 24 hours of room temperature recovery, and is cold-resistant (-5 +/-2 ℃ and 24 hours of room temperature recovery), does not delaminate, does not have obvious suspended matters, and all sensory indexes, physical and chemical indexes and sanitary indexes of the product all accord with corresponding national standards.

Besides the antibacterial and bactericidal effects, the mite cleaning agent also has good dirt-removing capacity and mite removing capacity, people can be frequently invaded by mites in life, and corpses, secretions and excretions of people can become allergens due to the fact that people are not easy to see by naked eyes, so that people can have diseases such as allergic dermatitis, asthmatic bronchitis, nephritis, allergic rhinitis and the like, and the human health is seriously harmed. Mites are more ubiquitous and may be distributed particularly on clothing in close contact with the human body. Usually, most of bacteriostatic lotion can only exert the stain removing effect, and can not achieve the mite removing effect. Experiments prove that the technical scheme of the invention has good decontamination and mite removal effects, so compared with the prior art, the invention has the following advantages:

1. the product of the invention belongs to physical bacteriostasis, and utilizes the fact that bacteria are attracted by cationic groups adsorbed on the surface of clothes, and the physical mode is bacteriostasis and sterilization.

2. Ecological mite killing: and (3) performing physical sterilization to form a positive electric field protective layer, cutting off the food source of the mites, and safely and efficiently removing the mites.

3. Strong detergency: the high-efficiency cleaning factor is broken through, the cleaning factor deeply enters the clothes fiber, and various stubborn stains are easily broken down, so that the clothes are clean as new from inside to outside.

4. The effect is durable: the formula is neutral, the clothes and the color are protected, the hands and the skin are protected, and the clothes and the skin are safe and environment-friendly.

The environment-friendly physical bacteriostatic lotion provided by the invention achieves the effect of mutual synergy when various raw materials are mixed for use, has a strong bacteriostatic effect, simultaneously plays a good bacteriostatic role on various strains, particularly has good effects on four strains of candida albicans, staphylococcus aureus, escherichia coli and bacillus subtilis, and solves the problem of poor antibacterial broad spectrum of chitosan derivatives in the prior art. Compared with the bacteriostatic products in the prior art, the bacteriostatic composition has a synergistic effect in the bacteriostatic aspect, has strong dirt-removing capability, has strong mite removing capability, can be suitable for daily life, has wide mite removing and bacteriostatic action range, is non-toxic and harmless, belongs to a natural antibacterial composition, and can exert the physical bacteriostatic effect to the maximum extent.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.

Example 1 preparation of an environmentally friendly physically bacteriostatic lotion

An environment-friendly physical bacteriostatic lotion comprises, by weight, 6% of a chitosan derivative, 20% of a chamomile extract, 0.6% of dodecyl dimethyl amine oxide, 0.3% of coconut oil diethanolamide, a proper amount of 1% citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water;

mixing a chitosan derivative and a chamomile extract according to a proportion, adding a certain amount of deionized water for dissolving, heating at 45 ℃ for 30 minutes, dissolving at the rotating speed of 800r/min, adding coconut oil diethanolamide and dodecyl dimethyl amine oxide according to the proportion while stirring at the rotating speed of 1200r/min at 26 ℃, simultaneously adding a proper amount of 1% citric acid solution to adjust the pH value to 6.8, ensuring that the stirring rotating speed is uniform in the process, fully mixing all substances, standing and packaging to obtain the environment-friendly physical bacteriostatic lotion.

Example 2 preparation of an environmentally friendly physically bacteriostatic lotion

An environment-friendly physical bacteriostatic lotion comprises 8% of chitosan derivative, 15% of chamomile extract, 0.4% of dodecyl dimethyl amine oxide, 0.4% of coconut oil diethanolamide, a proper amount of 1% of citric acid for adjusting the pH value to 6.0-7.0 and the balance of deionized water;

mixing a chitosan derivative and a chamomile extract according to a proportion, adding a certain amount of deionized water for dissolving, heating at 40 ℃ for 35 minutes, dissolving at the rotating speed of 1000r/min, adding coconut oil diethanolamide and dodecyl dimethyl amine oxide according to the proportion while stirring at the condition of 26 ℃ and the rotating speed of 1200r/min, simultaneously adding a proper amount of 1% citric acid solution to adjust the pH value to 6.8, ensuring that the stirring rotating speed is uniform in the process, fully mixing all substances, standing and packaging to obtain the environment-friendly physical bacteriostatic lotion.

Comparative example 1 preparation of bacteriostatic lotion

The bacteriostatic lotion comprises, by weight, 10% of a chitosan derivative, 10% of a chamomile extract, 0.4% of dodecyl dimethyl amine oxide, 0.4% of coconut oil diethanolamide, a proper amount of 1% citric acid for adjusting the pH to 7, and the balance of deionized water, and the method is the same as in example 1.

Comparative example 2 preparation of bacteriostatic lotion

The bacteriostatic lotion comprises 16 percent of chitosan derivative, 0.4 percent of dodecyl dimethyl amine oxide, 0.4 percent of coconut diethanolamide, a proper amount of 1 percent of citric acid for adjusting the pH to be 7.0, and the balance of deionized water by weight percent, and the method is the same as the example 1.

Comparative example 3 preparation of bacteriostatic lotion

The bacteriostatic lotion comprises 40 wt% of chamomile extract, 0.4 wt% of dodecyl dimethyl amine oxide, 0.4 wt% of coconut diethanolamide, a proper amount of 1 wt% of citric acid for adjusting the pH to 7.0, and the balance of deionized water, and the method is the same as that in example 1.

Experimental example 1 Effect of detergency ratio in examples of the present invention

The detergency of the washing liquids described in examples 1 and 2 of the present invention was measured according to the method specified in QB/T1224-2012, based on the measurement of the detergency and the circulating washing performance of the detergent for clothing in GB/T13174-2008.

Specific examples of the method include the following:

dyeing dirty cloth: preparing 4 percent (mass fraction) of starch water solution and stirring for 20 min. The solution was heated to boiling and kept boiling for 10min (with lid on while stirring). Mixing the starch solution, the ammonia-free caramel pigment and water according to the mass fraction of 1:1:1 to prepare the pollution liquid A. Adjusting the pressure of a small padder (F.2.3) to 0.3MPa, adjusting a sewage tank, pouring the polluted liquid A into the padder sewage tank, carrying out impregnation on white cotton cloth, and carrying out suspension and air drying. Preheating the oven to 100 +/-2 ℃, and hanging the dirty cloth in the oven to heat for 30 min.

Preservation and use of starch dirty cloth: the product is stored at 3-5 deg.C, and can be used for measuring detergency after 24h aging, and can be cut into test size according to standard requirements. The quality guarantee period of the dirty cloth is three months.

Meanwhile, carbon black dirty cloth, sebum dirty cloth and the like are dyed.

And (3) determining the detergency: taking 6 pieces of circular dirty cloth test pieces with the diameter of 4cm, stacking the 6 pieces of circular dirty cloth test pieces in different culture dishes respectively, taking one piece of dirty cloth as a group, weighing a washing liquid, adding 2ml of hard water with the concentration of 250mg/kg into a beaker for dilution, uniformly dripping the uniformly mixed washing liquid on each piece of dirty cloth by using drip irrigation according to the stacking sequence of the starch dirty cloth, the carbon black dirty cloth and the sebum dirty cloth test pieces, slightly turning by using tweezers to completely wet the dirty cloth, controlling the dripping time within 1min, and standing the soaked dirty cloth test pieces for 5 min. 200ml of 250mg/kg hard water heated to 30 ℃ is taken, samples remained in a dropper, a beaker and a culture dish and the soaked dirty cloth test piece are all transferred into a test bathtub, and 800ml of 250mg/kg hard water at 30 ℃ is added. Meanwhile, a blank control is adopted to treat the dirty cloth test piece for comparison and measurement, and the test data are shown in the following table 1.

Table 1: influence of bacteriostatic lotion on removal effect of dirt

Decontamination ratio	Starch dirty cloth	Carbon black dirt cloth	Sebum dirt cloth
				Example 1	1.01	1.52	0.84
Example 2	0.99	1.50	0.82

It can be seen that the soil removal ability of the examples in the application is high, which indicates that the soil removal ability is strong and stable.

Experimental example 2 comparative study on mite-removing effect in the present invention

The mite removing effect test of the bacteriostatic lotion prepared in the above example 1-2,

the specific method comprises the following steps: and (3) taking dust mites as test objects, adding water into the laundry detergent for diluting to 20 times, and filling the laundry detergent into a spraying device to be used as a spraying agent. A closed cylinder device with a glass material is selected, and after spraying for 10 minutes, the glass pulling plate is taken out and timing is carried out immediately. The test procedure was as follows: 100 mites of 8cm diameter petri dishes were placed in a closed cylinder test apparatus. Spraying 5g of the solution into the cylinder by a spraying device, and observing the number of knocked down test insects by a microscope after 10 minutes. Finally, the knock-down rate was calculated for 10 minutes and 24 hour mortality was observed, and the results are shown in table 2.

TABLE 2 Effect of bacteriostatic lotions on mite removal

	Knock down rate of 10 minutes	Mortality rate of 24 hours
			Example 1	87.09	88.89
Example 2	85.14	86.51

As can be seen from table 2, the removal of mites by examples 1 to 2 of the present invention was uniform and superior in the effects within 10 minutes and within 24 hours, suggesting that the antibacterial water composition of the present invention has an improved efficacy in removing mites.

Experimental example 3 comparison of antibacterial experiments

The bacteriostatic activity of Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC8739), Staphylococcus aureus (ATCC 6538) and Candida albicans (ATCC10231) was tested for 5 bacteriostatic lotions of examples 1 to 2 and comparative examples 1 to 3 of the present application, according to the regulations of the Ministry of public health of the people's republic of China (2002).

The preparation of the bacterial suspension comprises the step of testing the bacteria content of Escherichia coli and staphylococcus aureus to be 1 × 10 according to the disinfection technical Specification of the Ministry of health⁸cfu/mL-5×10⁸cfu/mL, the bacteria content of the killing test of candida albicans and bacillus subtilis is 1 × 10⁷cfu/mL-5×10⁷cfu/mL。

Each test bacterium was tested using different lotions according to examples 1 to 2 and comparative examples 1 to 3, respectively, and the test bacteria were Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis, respectively. Each example test example is grouped as follows: (1) example 1 wash solution + bacterial suspension, (2) example 1 wash solution + bacterial suspension + neutralizing agent, (3) neutralizing agent + bacterial suspension, (4) (laundry detergent solution example 1 wash solution + neutralizing agent + bacterial suspension, (5) TPS + bacterial suspension, (6) the same batch of TPS, neutralizing agent, culture medium used in the test, action time 10min, test repetition 3 times, test temperature 19 ℃ -21 ℃, examples 1-2 and comparative examples 1-3 test results are shown in table 3.

TABLE 3 results of bacteriostatic experiments in examples 1-2 and comparative examples 1-3

	Example 1	Example 2	Comparative example 1	Comparative example 2	Comparative example 3
						Candida albicans	91.6	90.1	87.1	76.5	85.1
Staphylococcus aureus	97.2	97.0	85.3	87.4	76.9
						Escherichia coli	96.6	96.4	86.9	86.1	78.3
Bacillus subtilis	89.1	90.2	90.1	78.2	82.4

As shown in the experimental results in Table 3, after the bacteriostatic lotion of the groups of examples 1-2 and comparative examples 1-3 acts for 10min, the bacteriostatic rates of Escherichia coli, Staphylococcus aureus, Candida albicans and Bacillus subtilis can reach over 75%, and the bacteriostatic activity is strong.

Further comparing, as can be seen from table 3, the bacteriostatic rate of the environmentally-friendly physical bacteriostatic lotion prepared in examples 1-2 of the present invention against staphylococcus aureus, escherichia coli, candida albicans, and bacillus subtilis can reach more than 90%, which proves that the hand sanitizer has strong bacteriostatic ability. Compared with comparative examples 1-3, the chitosan derivative and the chamomile extract in the comparative example 1 are adjusted in dosage, so that the bacteriostatic power of the compound preparation on bacillus subtilis, candida albicans, staphylococcus aureus and escherichia coli is reduced; the comparative example 2 only contains the chitosan derivative, and has lower bacteriostatic rate on bacillus subtilis and candida albicans, while the comparative example 3 only contains the chamomile extract, and the bacteriostatic action on staphylococcus aureus, escherichia coli and candida albicans is lower than that of the examples 1-2, so that the chitosan derivative and the chamomile extract show synergistic bacteriostatic effect in a certain proportion range, and the bacteriostatic rate can be greatly improved.

Obviously, the effect experiments performed in the above examples are only examples for clearly illustrating the present invention, and do not limit the embodiments and the application scope. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. It is neither necessary nor exhaustive here for all embodiments and effects of use. And obvious variations or modifications therefrom are within the scope of the invention.

Claims

1. An environment-friendly physical bacteriostatic lotion comprises the following components in percentage by weight: 6-8% of chitosan derivative, 15-20% of chamomile extract, 0.4-0.6% of dodecyl dimethyl amine oxide, 0.3-0.4% of coconut oil diethanolamide, a proper amount of 1% citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water;

the preparation method of the chitosan derivative comprises the following steps: dissolving N-methyl-N-dodecyl-N, N-dihydroxyethyl ammonium bromide by using a proper amount of chloroform, adding 4-dimethylaminopyridine and triethylamine, cooling in an ice-water bath, then dropwise adding p-methylbenzenesulfonyl chloride dissolved in the chloroform into the mixed solution, wherein the molar ratio of the N-methyl-N-dodecyl-N, N-dihydroxyethyl ammonium bromide to the p-methylbenzenesulfonyl chloride to the triethylamine to the 4-dimethylaminopyridine is 3:1:1.5:0.05, stirring at room temperature after ice-bath reaction until the reaction is complete, decompressing and steaming to remove the chloroform to obtain a white solid intermediate 1, and drying in vacuum for later use; dissolving N-benzylidene-chitosan in isopropanol, adding NaOH solution, stirring, adding the intermediate 1 into the solution, heating to a certain temperature for reaction for enough time, cooling, adding absolute ethyl alcohol, performing suction filtration, adding acetone: washing the ethanol mixed solution for 3 times, drying in vacuum to obtain an intermediate 2, dissolving the intermediate 2 in 10mL of 0.25mol/L hydrochloric acid/ethanol solution, stirring at room temperature for reaction, performing suction filtration, washing with acetone for 3 times, and drying and purifying to obtain a chitosan derivative;

2. The environment-friendly physical bacteriostatic lotion according to claim 1, which comprises the following components, by weight, 6% of chitosan derivative, 20% of chamomile extract, 0.6% of dodecyl dimethyl amine oxide, 0.3% of coconut diethanolamide, a proper amount of 1% of citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water.

3. The environment-friendly physical bacteriostatic lotion according to claim 1, which comprises 8 percent of chitosan derivative, 15 percent of chamomile extract, 0.4 percent of dodecyl dimethyl amine oxide, 0.4 percent of coconut diethanolamide, a proper amount of 1 percent of citric acid for adjusting the pH value to 6.0-7.0, and the balance of deionized water.

4. A preparation method of an environment-friendly physical bacteriostatic lotion according to any one of claims 1 to 3, characterized by mixing a chitosan derivative and a chamomile extract in proportion, adding a certain amount of deionized water for dissolution, heating at 40-50 ℃ for 20-40 minutes, dissolving at the rotation speed of 800-1000r/min, adding cocodiethanolamide and dodecyl dimethyl amine oxide according to the proportion while stirring at the temperature of 26-28 ℃ and the rotation speed of 1200r/min, simultaneously adding a proper amount of 1% citric acid solution to adjust the pH value to 6-7, adjusting the pH value to 6.8, ensuring that the stirring rotation speed is uniform in the process, fully mixing the substances, standing and packaging to obtain the environment-friendly physical bacteriostatic lotion.

5. Use of the eco-friendly physical bacteriostatic lotion according to any one of claims 1 to 3 in household chemicals or washing products.

6. The use of claim 5, wherein the daily chemical or washing product is a laundry detergent with antibacterial effect, and is prepared by soaking 30-50ml of the product in 2-3kg of water for 15-20 min, and washing normally.