CN108703068B - 去除慈姑培养过程中内生菌的方法、培养方法以及应用 - Google Patents
去除慈姑培养过程中内生菌的方法、培养方法以及应用 Download PDFInfo
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- CN108703068B CN108703068B CN201810297284.3A CN201810297284A CN108703068B CN 108703068 B CN108703068 B CN 108703068B CN 201810297284 A CN201810297284 A CN 201810297284A CN 108703068 B CN108703068 B CN 108703068B
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- 238000012136 culture method Methods 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title abstract description 51
- 239000001963 growth medium Substances 0.000 claims abstract description 60
- 229960000723 ampicillin Drugs 0.000 claims abstract description 59
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims abstract description 59
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 claims abstract description 58
- 229960002385 streptomycin sulfate Drugs 0.000 claims abstract description 58
- 238000009630 liquid culture Methods 0.000 claims abstract description 27
- 230000035755 proliferation Effects 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims description 30
- 229930006000 Sucrose Natural products 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 14
- 238000005286 illumination Methods 0.000 claims description 14
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- 229940113118 carrageenan Drugs 0.000 claims description 8
- 235000010418 carrageenan Nutrition 0.000 claims description 8
- 229920001525 carrageenan Polymers 0.000 claims description 8
- 239000000679 carrageenan Substances 0.000 claims description 8
- 230000006698 induction Effects 0.000 claims description 8
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims description 8
- 230000001902 propagating effect Effects 0.000 claims description 5
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- 239000003242 anti bacterial agent Substances 0.000 abstract description 3
- 229940088710 antibiotic agent Drugs 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 240000009132 Sagittaria sagittifolia Species 0.000 description 15
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- 239000002609 medium Substances 0.000 description 13
- 229960004793 sucrose Drugs 0.000 description 10
- 238000011109 contamination Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 239000000843 powder Substances 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
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- 230000000249 desinfective effect Effects 0.000 description 2
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- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
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- OTVAEFIXJLOWRX-NXEZZACHSA-N thiamphenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CO)NC(=O)C(Cl)Cl)C=C1 OTVAEFIXJLOWRX-NXEZZACHSA-N 0.000 description 1
- 229960003053 thiamphenicol Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
硫酸链霉素浓度(mg/L) | 组培苗污染率% | 继代增殖倍数 | 生根率% |
50 | 42 | 1.0 | 85 |
100 | 37 | 2.3 | 85 |
150 | 32 | 2.1 | 85 |
200 | 23 | 2.1 | 72 |
400 | 22 | 1.0 | 70 |
氨苄西林浓度(mg/L) | 组培苗污染率% | 继代增殖倍数 | 生根率% |
50 | 62 | 1.0 | 73 |
100 | 45 | 2.1 | 82 |
150 | 42 | 2.0 | 80 |
200 | 35 | 2.0 | 71 |
400 | 35 | 1.0 | 70 |
Claims (3)
Priority Applications (1)
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CN201810297284.3A CN108703068B (zh) | 2018-04-04 | 2018-04-04 | 去除慈姑培养过程中内生菌的方法、培养方法以及应用 |
Applications Claiming Priority (1)
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CN201810297284.3A CN108703068B (zh) | 2018-04-04 | 2018-04-04 | 去除慈姑培养过程中内生菌的方法、培养方法以及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108703068A CN108703068A (zh) | 2018-10-26 |
CN108703068B true CN108703068B (zh) | 2021-09-10 |
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CN201810297284.3A Expired - Fee Related CN108703068B (zh) | 2018-04-04 | 2018-04-04 | 去除慈姑培养过程中内生菌的方法、培养方法以及应用 |
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CN (1) | CN108703068B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112655557B (zh) * | 2020-12-22 | 2022-08-30 | 杭州师范大学 | 一种抑制离体培养吊瓜苗内生菌的方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5723720A (en) * | 1995-06-07 | 1998-03-03 | J. R. Simplot Company | Process for the development of endophyte-infected plants |
US20070074313A1 (en) * | 2005-09-16 | 2007-03-29 | J.R. Simplot Company | Native antibiotic resistance genes |
CN101554137A (zh) * | 2009-04-09 | 2009-10-14 | 白永兴 | 慈菇组培育苗方法 |
CN103070073A (zh) * | 2013-01-22 | 2013-05-01 | 武汉市蔬菜科学研究所 | 试管慈姑组织培养快速繁殖的方法 |
CN103609454B (zh) * | 2013-12-04 | 2016-03-09 | 福建农林大学 | 一种降低茶树组织培养过程中外植体污染的方法 |
CN104770302B (zh) * | 2015-04-23 | 2016-08-10 | 福建农林大学 | 一种常春油麻藤快速繁殖方法 |
CN106879462B (zh) * | 2017-01-20 | 2019-04-16 | 浙江理工大学 | 一种长喙毛茛泽泻微繁方法 |
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2018
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