CN108680630A - 一种直接双酶电极及其在植酸酶活性测定中的应用 - Google Patents
一种直接双酶电极及其在植酸酶活性测定中的应用 Download PDFInfo
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- CN108680630A CN108680630A CN201810411570.8A CN201810411570A CN108680630A CN 108680630 A CN108680630 A CN 108680630A CN 201810411570 A CN201810411570 A CN 201810411570A CN 108680630 A CN108680630 A CN 108680630A
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- 229940085127 phytase Drugs 0.000 title claims abstract description 50
- 230000000694 effects Effects 0.000 title claims abstract description 37
- 238000005259 measurement Methods 0.000 title claims abstract description 30
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- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000007711 solidification Methods 0.000 claims abstract description 8
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- 239000000467 phytic acid Substances 0.000 claims description 11
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- 239000012530 fluid Substances 0.000 claims description 9
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims description 6
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 claims description 5
- 108010093894 Xanthine oxidase Proteins 0.000 claims description 5
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- 230000033116 oxidation-reduction process Effects 0.000 claims description 5
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- 239000007974 sodium acetate buffer Substances 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 229930010555 Inosine Natural products 0.000 claims description 4
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- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 4
- 239000004299 sodium benzoate Substances 0.000 claims description 4
- 235000010234 sodium benzoate Nutrition 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 2
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- 229930182470 glycoside Natural products 0.000 claims 1
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- 230000008901 benefit Effects 0.000 abstract description 20
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- 238000011084 recovery Methods 0.000 description 8
- 239000012528 membrane Substances 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 3
- 238000001952 enzyme assay Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
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- 241000894006 Bacteria Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- BGAKIZCYMPPJEH-UHFFFAOYSA-N [O].O=C1NC=NC2=C1NC=N2 Chemical compound [O].O=C1NC=NC2=C1NC=N2 BGAKIZCYMPPJEH-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 238000007385 chemical modification Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
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CN201810411570.8A CN108680630B (zh) | 2018-05-02 | 2018-05-02 | 一种直接双酶电极及其在植酸酶活性测定中的应用 |
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CN108680630A true CN108680630A (zh) | 2018-10-19 |
CN108680630B CN108680630B (zh) | 2020-04-10 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112415062A (zh) * | 2020-11-27 | 2021-02-26 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极系统及使用电极系统对乙醇检测的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007003280A (ja) * | 2005-06-22 | 2007-01-11 | Techno Medica Co Ltd | 電極構造体及びそれを含む体液中のリン酸測定用酵素センサー |
CN102901767A (zh) * | 2012-10-24 | 2013-01-30 | 南开大学 | 以两相酶生物传感器实时监测葡萄糖氧化酶活力的方法 |
CN106442681A (zh) * | 2016-11-22 | 2017-02-22 | 山东省科学院生物研究所 | 基于双酶固定化技术的甘油快速测定生物传感器的方法 |
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2018
- 2018-05-02 CN CN201810411570.8A patent/CN108680630B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007003280A (ja) * | 2005-06-22 | 2007-01-11 | Techno Medica Co Ltd | 電極構造体及びそれを含む体液中のリン酸測定用酵素センサー |
CN102901767A (zh) * | 2012-10-24 | 2013-01-30 | 南开大学 | 以两相酶生物传感器实时监测葡萄糖氧化酶活力的方法 |
CN106442681A (zh) * | 2016-11-22 | 2017-02-22 | 山东省科学院生物研究所 | 基于双酶固定化技术的甘油快速测定生物传感器的方法 |
Non-Patent Citations (2)
Title |
---|
SAMUEL B. ADELOJU ET AL: "Fabrication of a bilayer potentiometric phosphate biosensor by cross-link immobilization with bovine serum albumin and glutaraldehyde", 《ANALYTICA CHIMICA ACTA》 * |
孙士青 等: "肌苷酶电极生物传感器", 《生物工程学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112415062A (zh) * | 2020-11-27 | 2021-02-26 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极系统及使用电极系统对乙醇检测的方法 |
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CN108680630B (zh) | 2020-04-10 |
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Effective date of registration: 20240313 Address after: E-E-101, Dezhou Zhongyuan Technology Innovation and Entrepreneurship Park, No. 6596 Dongfanghong East Road, Yuanqiao Town, Economic and Technological Development Zone, Dezhou City, Shandong Province, 253000 Patentee after: Zhigan Biotechnology (Shandong) Co.,Ltd. Country or region after: China Address before: No. 28789, Jingshi East Road, Caishi Town, Licheng District, Jinan City, Shandong Province, 250014 Patentee before: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES Country or region before: China |