CN108680630B - 一种直接双酶电极及其在植酸酶活性测定中的应用 - Google Patents
一种直接双酶电极及其在植酸酶活性测定中的应用 Download PDFInfo
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- CN108680630B CN108680630B CN201810411570.8A CN201810411570A CN108680630B CN 108680630 B CN108680630 B CN 108680630B CN 201810411570 A CN201810411570 A CN 201810411570A CN 108680630 B CN108680630 B CN 108680630B
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- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 6
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
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- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- 229910019142 PO4 Inorganic materials 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 101710154508 Purine nucleoside phosphorylase 1 Proteins 0.000 description 1
- 101710132082 Pyrimidine/purine nucleoside phosphorylase Proteins 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000003028 enzyme activity measurement method Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
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- 230000007062 hydrolysis Effects 0.000 description 1
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- 238000011081 inoculation Methods 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
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- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
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- 239000007222 ypd medium Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
Abstract
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CN201810411570.8A CN108680630B (zh) | 2018-05-02 | 2018-05-02 | 一种直接双酶电极及其在植酸酶活性测定中的应用 |
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CN108680630B true CN108680630B (zh) | 2020-04-10 |
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CN112415062B (zh) * | 2020-11-27 | 2022-09-16 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极系统及使用电极系统对乙醇检测的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007003280A (ja) * | 2005-06-22 | 2007-01-11 | Techno Medica Co Ltd | 電極構造体及びそれを含む体液中のリン酸測定用酵素センサー |
CN102901767A (zh) * | 2012-10-24 | 2013-01-30 | 南开大学 | 以两相酶生物传感器实时监测葡萄糖氧化酶活力的方法 |
CN106442681A (zh) * | 2016-11-22 | 2017-02-22 | 山东省科学院生物研究所 | 基于双酶固定化技术的甘油快速测定生物传感器的方法 |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007003280A (ja) * | 2005-06-22 | 2007-01-11 | Techno Medica Co Ltd | 電極構造体及びそれを含む体液中のリン酸測定用酵素センサー |
CN102901767A (zh) * | 2012-10-24 | 2013-01-30 | 南开大学 | 以两相酶生物传感器实时监测葡萄糖氧化酶活力的方法 |
CN106442681A (zh) * | 2016-11-22 | 2017-02-22 | 山东省科学院生物研究所 | 基于双酶固定化技术的甘油快速测定生物传感器的方法 |
Non-Patent Citations (2)
Title |
---|
Fabrication of a bilayer potentiometric phosphate biosensor by cross-link immobilization with bovine serum albumin and glutaraldehyde;Samuel B. Adeloju et al;《Analytica Chimica Acta》;20110212;第691卷;89-94 * |
肌苷酶电极生物传感器;孙士青 等;《生物工程学报》;20081025;第24卷(第10期);1796-1800 * |
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Effective date of registration: 20240313 Address after: E-E-101, Dezhou Zhongyuan Technology Innovation and Entrepreneurship Park, No. 6596 Dongfanghong East Road, Yuanqiao Town, Economic and Technological Development Zone, Dezhou City, Shandong Province, 253000 Patentee after: Zhigan Biotechnology (Shandong) Co.,Ltd. Country or region after: China Address before: No. 28789, Jingshi East Road, Caishi Town, Licheng District, Jinan City, Shandong Province, 250014 Patentee before: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES Country or region before: China |
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