A kind of moisturizing whitening mask and preparation method thereof
Technical field
The present invention relates to a kind of moisturizing whitening mask and preparation method thereof, belong to cosmetic technical field.
Background technology
Cosmetics refer to imposing on human body surface with smearing, sprinkling or other methods, to reach cleaning, skin care, beauty, removing
The chemical products of bad smell and modification purpose, as cosmetics are to natural sex, safety, science, comprehensive, practicability side
To development, the status of the exploitations of natural cosmetics in 21 century cosmetics industry is more and more important.Currently, from the country
From the point of view of the production and consumption of outer cosmetics, using natural material, natural cosmetics of the exploitation containing natural material have been modernizations
Cosmetic industrial expansion trend.In recent years, either domestic or manufacturer of foreign countries and consumer are to the emerging of natural cosmetics
Interest is all being increasingly enhanced.Its main cause, which is exactly consumer, fosters suspicion psychology to the safety of synthetic, causes cosmetics
Occur as possible less with or without synthetic, and production firm is made competitively to brag about and adapted natural materials.
Effect of the moisturizer in cosmetics is to maintain or increase the water content of keratoderma, is improved because of cuticula water shortage
Cause dry skin and it is resulting itch, the clinical symptoms such as furfur.Moisturizer by its source can be divided into natural moisture preserving agent and
Synthesize moisturizer;Moisturizer, emollient, deep moisturizing agent can be divided by its mechanism of action again.It is used in cosmetics at present
Moisturizer be mainly the kinds such as polyalcohol, sorbierite, lactic acid and its sodium salt, belong to chemicals mostly.Green natural moisturizing
Oneself becomes the theme of contemporary beauty and skin care.Therefore, people, which yearn for, returns to naturally, starting the method for attention nature beauty.From natural
The natural moisture preserving agent substituted chemistry synthesis moisturizer with nutrition and moisturizing dual property extracted in substance, meets people's recurrence
The requirement of the Nature is the trend of the following moisturizer development.
Invention content
The present invention provides a kind of mask products with moisturizing and whitening function, and natural extract warp is mainly utilized
Everfermentation handles to be used as active constituent.Main component in the mask product is to use aloe extract to play anti-inflammatory, protect
Wet effect is played the role of inhibiting tyrosinase and then skin whitening using Chinese gooseberry fermentate, utilizes sunglo iron
Blue fermentate plays the role of moisturizing.
To achieve the goals above, the present invention adopts the following technical scheme that:
A kind of moisturizing whitening mask includes:Base material and the active constituent being overlying on base material wrap in the active constituent
It includes:Aloe extract, Chinese gooseberry fermentate, sunglo iron orchid fermentate.
Further, the preparation method of the aloe extract includes the following steps:After fresh Chinese aloe leaf is cleaned,
Epidermis is removed, inner gelatinous object is squeezed, the liquid squeezed passes through after filter-cloth filtering, obtains extract solution from aloe.
Further, the preparation method of the Chinese gooseberry fermentate includes the following steps:Chinese gooseberry is gone
After skin, internal pulp breaks into pulp, by pulp and deionized water according to weight ratio 1:10~20 are uniformly mixed, and bacterial strain is added
It ferments, the supernatant to ferment passes through after ultrafiltration membrance filter, and filtrate carries out carry out distillation operation, removes in zymotic fluid
Ethyl alcohol and other fusels, the bottoms material obtained after rectifying be dried in vacuo, obtain Chinese gooseberry fermentate.
Further, the fermentation process time is 30~60h, and fermentation temperature is 25~30 DEG C;Vacuum drying temperature
80~85 DEG C of degree;Fusel is again by ceramic membrane contactor and ethylenediamine and isooctane according to weight ratio 0.5~2:100 mix
Fluid Contacting contacted, remove fusel in H2After S, it is recycled.
Further, the preparation method of the sunglo iron orchid fermentate includes the following steps:By the blade of sunglo iron orchid
After cleaning, it is ground into wet powder, then by wet powder and deionized water according to weight ratio 1:10~20 be uniformly mixed, be added carbon source and
Nitrogen source and bacterial strain ferment, and the supernatant to ferment passes through after ultrafiltration membrance filter, and filtrate is dried in vacuo, and is obtained
Sunglo iron orchid fermentate.
Further, the fermentation process time is 20~30h, and fermentation temperature is 25~30 DEG C.
Further, the sunglo iron orchid fermentate is present in facial mask using liposome as carrier.
Further, the preparation method of the liposome includes the following steps:By weight, by soybean lecithin 30
~60 parts, 5~20 parts of cholesterol, 0.2~2 part of vitamin E, 0.4~1.8 part of Tween 80, the mixing of 0.2~1 part of sorbester p17 it is equal
It is even, it is redispersed in 150~350 parts of ether, adds 0.5~3 part of dispersant, be uniformly mixed as oil under ultrasonication
Phase;Sunglo iron orchid fermentate is dissolved according to 1~10wt% of concentration in deionized water, as water phase, then in the effect of ultrasound
The lower water phase by 20~60 parts is added dropwise in oil phase, then vacuum distillation removes ether at 25~35 DEG C, then in obtained gel
It is middle that 300~600 parts of phosphate buffer solution is added, at 25~35 DEG C vacuum distillation make volume be reduced to original 1/3~
1/5, after supersound process, obtain liposome turbid liquor.
The dispersant is polyethylene glycol.
The preparation method of above-mentioned moisturizing whitening mask, includes the following steps:
By weight, in 30~70 parts of deionized water, 0.05~0.3 part of 5~15 parts of moisturizer and solubilizer is added, rises
Temperature is stirred evenly to 40~70 DEG C of 15~45min of holding;Then, continuously add take 0.05~0.5 part of aloe extract, in
0.1~1 part of magnificent macaque Fructus Persicae fermented product, 0.05~0.4 part of 0.1~1.5 part of liposome, the preservative of sunglo iron orchid fermentate, perfume
Smart 0.02~0.2 part, 0.02~0.2 part of citric acid, after stirring evenly, add 30~70 parts of deionized water, after stirring evenly, obtain
Facial mask liquid is infiltrated on facial mask special non-woven fabric by facial mask liquid again, then carry out aseptic packaging to get.
Further, the moisturizer is selected from glycerine, propylene glycol or 1,3 butanediols.
Further, the solubilizer is selected from Cremophor RH40, polysorbas20 or polysorbate40.
Further, the preservative is selected from parabens, methylisothiazolinone or Phenoxyethanol.
The present invention also provides a kind of preparation method of Chinese gooseberry fermentate and its purposes in cosmetics.
The present invention also provides a kind of preparation method of sunglo iron orchid fermentate, the systems of the liposome of sunglo iron orchid fermentate
Preparation Method and their purposes in cosmetics.
Advantageous effect
The facial mask provided in the present invention is utilized aloe extract moisturizing, anti-inflammatory, antibacterial effect, while assisting with Chinese Mi
Monkey Fructus Persicae fermented product is realized scavenging hydroxyl and is inhibited tyrosinase activity, and is carried in addition, being played by sunglo iron orchid fermentate
High skin elasticity and the effect for keeping moisture of skin.Above-mentioned natural plants make active ingredient therein after everfermentation
It is further decomposed as small molecule, keeps its whitening, moistening effect more preferable, by the way that sunglo iron orchid fermentate is prepared in liposome
In, it will be apparent that improve its moistening effect.
Description of the drawings
Fig. 1 is the TEM figures of sunglo iron orchid fermentate liposome turbid liquor.
Fig. 2 is the grain size distribution of sunglo iron orchid fermentate liposome turbid liquor.
Fig. 3 is the gas phase collection of illustrative plates for the fusel that Fructus actinidiae chinensis fermented solution rectification and purification obtains in the process, what each composition represented
The ownership and retention time isopropanol 7.152 at peak, normal propyl alcohol 9.033, sec-butyl alcohol 10.283, isobutanol 11.254, n-butanol
12.283, ethyl alcohol 13.044,2- methyl-1s butanol 15.024, -2 amylalcohol 15.674 of 4- methyl, n-amyl alcohol 16.076, benzyl alcohol
24.381。
Fig. 4 is the comparison of Chinese gooseberry fermentate and water extract inhibitory activity against tyrosinase.
Fig. 5 is the comparison that Chinese gooseberry fermentate and water extract DPPH remove IC50 values.
Fig. 6 is the moistening effect figure of sunglo iron orchid fermentate liposome turbid liquor.
Specific implementation mode
The solution of the present invention is described in detail below by way of specific implementation mode, wherein the percentage is in Wu Te
Do not mentionlet alone it is bright in the case of all refer to mass percent.
In the present invention, aloin that aloe extract contains, Aloesin have the efficiency of many-sided beautifying skin,
Moisturizing, anti-inflammatory, antibacterial, antipruritic, antiallergy, softening skin tool have certain effect, wherein a variety of elimination superoxides contained
The ingredient of free radical can make skin delicate, flexible, have anti-corrosion and delay to decline such as superoxide dismutase, catalase
The effect of waiting for a long time.Chinese gooseberry fermentate has the function of scavenging hydroxyl and inhibits tyrosinase activity.Sunglo iron is blue
The glycine betaine contained in fermentate can effectively improve skin elasticity and keep the effect of moisture of skin.
The preparation of 1 aloe extract of embodiment
Fresh aloe leaf is taken, epidermis will be removed, inner gelatinous object is squeezed, the liquid squeezed passes through 200 mesh
Filter-cloth filtering after, obtain extract solution from aloe.
The preparation of 2 Chinese gooseberry fermentate of embodiment
A certain amount of Angel brewer's yeast BF16 is taken, YPD culture mediums are seeded to(Yeast extract 1%, peptone 2%, glucose 2%,
It is mass fraction)In, it carries out strain and spreads cultivation, obtain yeast liquid.
Chinese gooseberry is removed the peel, takes the pulp of 1Kg to break into pulp, then pulp is added into 10Kg deionized waters, then
200mL yeast bacteria liquid is added, after adjusting pH to 4.0~4.5 or so with sodium bicarbonate, in 28~30 DEG C in fermentation cylinder for fermentation 50
h.Obtained supernatant ferment after quartz filter is filtered, is re-fed into the purpose ceramic-film filter of 50 μm of average pore size
In be filtered except thalline, pressure 0.2MPa during ceramic membrane filter, ceramic membrane filtrate carries out distillation operation, removes zymotic fluid
In ethyl alcohol and other fusels, rectifying column bottom temperature is 105 DEG C, and column top temperature is 95 DEG C, and totally 67 blocks of column plates, feed entrance point exist
55th piece of plate height, ethyl alcohol is obtained from recovered overhead, recycles to obtain fusel, rectifying column atmospheric operation from the 59th block of column plate;
The bottoms material obtained after rectifying is subjected to the vacuum drying at 80~85 DEG C, obtains Chinese gooseberry fermentate.
Fusel passes through gas chromatographic analysis, and GC collection of illustrative plates is as shown in figure 3, composition is:Isopropanol 1.22%, normal propyl alcohol
0.45%, sec-butyl alcohol 1.34%, isobutanol 0.74%, n-butanol 4.83%, ethyl alcohol 43.92%, 2- methyl-1s butanol 1.18%, 4- first
- 2 amylalcohol 1.58% of base, n-amyl alcohol 0.86%, benzyl alcohol 0.32%, in addition also containing the H generated in fermentation process2S 0.23%;It will
Fusel is sent into ceramic membrane contactor, the average hole of the single-tube ceramic membranes used in ceramic membrane contactor under conditions of 30 DEG C
Diameter is 20nm, is flowed for fusel on the outside of film, in Bottomhole pressure ethylenediamine and the isooctane by weight 1 of film:100 prepare
Solution make the H in fusel after absorption2S, which is reduced to, to be not detected, and obtained fusel more solito carries out component
Separation, can recycle.
The preparation of 3 Chinese gooseberry fermentate of embodiment
A certain amount of Angel brewer's yeast BF16 is taken, YPD culture mediums are seeded to(Yeast extract 1%, peptone 2%, glucose 2%,
It is mass fraction)In, it carries out strain and spreads cultivation, obtain yeast liquid.
Chinese gooseberry is removed the peel, takes the pulp of 1Kg to break into pulp, then pulp is added into 12Kg deionized waters, then
220mL yeast bacteria liquid is added, after adjusting pH to 4.0~4.5 or so with sodium bicarbonate, in 30~32 DEG C in fermentation cylinder for fermentation
45h.Obtained supernatant ferment after quartz filter is filtered, is re-fed into the ceramic membrane mistake of 200 μm of average pore size
It is filtered in filter except thalline, pressure 0.3MPa during ceramic membrane filter, ceramic membrane filtrate carries out distillation operation, removal hair
Ethyl alcohol in zymotic fluid and other fusels, rectifying column bottom temperature is 103 DEG C, and column top temperature is 93 DEG C, totally 70 blocks of column plates, feed position
It sets in the 58th piece of plate height, ethyl alcohol is obtained from recovered overhead, recycle to obtain fusel, rectifying column normal pressure behaviour from the 62nd block of column plate
Make;The bottoms material obtained after rectifying is subjected to the vacuum drying at 80~85 DEG C, obtains Chinese gooseberry fermentate.
Fusel passes through gas chromatographic analysis, and composition is:Isopropanol 1.34%, normal propyl alcohol 0.42%, sec-butyl alcohol 1.23%, isobutyl
Alcohol 0.65%, n-butanol 4.98%, ethyl alcohol 45.35%, 2- methyl-1s butanol 1.35%, -2 amylalcohol 1.73% of 4- methyl, n-amyl alcohol
0.98%, benzyl alcohol 0.25%, in addition also containing the H generated in fermentation process2S 0.32%;By fusel under conditions of 32 DEG C, send
Enter in ceramic membrane contactor, the average pore size of the single-tube ceramic membranes used in ceramic membrane contactor is miscellaneous on the outside of film for 50nm
Alcohol is flowed, in Bottomhole pressure ethylenediamine and the isooctane by weight 1 of film:120 solution prepared, after absorption,
Make the H in fusel2S, which is reduced to, to be not detected, and obtained fusel more solito detaches component, can recycle.
The preparation of 4 Chinese gooseberry fermentate of embodiment
A certain amount of Angel brewer's yeast BF16 is taken, YPD culture mediums are seeded to(Yeast extract 1%, peptone 2%, glucose 2%,
It is mass fraction)In, it carries out strain and spreads cultivation, obtain yeast liquid.
Chinese gooseberry is removed the peel, takes the pulp of 1Kg to break into pulp, then pulp is added into 15Kg deionized waters, then
280mL yeast bacteria liquid is added, after adjusting pH to 4.0~4.5 or so with sodium bicarbonate, in 26~28 DEG C in fermentation cylinder for fermentation
35h.Obtained supernatant ferment after quartz filter is filtered, is re-fed into the ceramic membrane filter of 50 μm of average pore size
It is filtered in device except thalline, pressure 0.25MPa during ceramic membrane filter, ceramic membrane filtrate carries out distillation operation, removal hair
Ethyl alcohol in zymotic fluid and other fusels, rectifying column bottom temperature is 102 DEG C, and column top temperature is 98 DEG C, totally 60 blocks of column plates, feed position
It sets in the 50th piece of plate height, ethyl alcohol is obtained from recovered overhead, recycle to obtain fusel, rectifying column normal pressure behaviour from the 56th block of column plate
Make;The bottoms material obtained after rectifying is subjected to the vacuum drying at 80~85 DEG C, obtains Chinese gooseberry fermentate.
Fusel passes through gas chromatographic analysis, and composition is:Isopropanol 1.53%, normal propyl alcohol 0.74%, sec-butyl alcohol 1.12%, isobutyl
Alcohol 0.46%, n-butanol 4.28%, ethyl alcohol 41.27%, 2- methyl-1s butanol 1.03%, -2 amylalcohol 1.26% of 4- methyl, n-amyl alcohol
0.32%, benzyl alcohol 0.42%, in addition also containing the H generated in fermentation process2S 0.28%;By fusel under conditions of 32 DEG C, send
Enter in ceramic membrane contactor, the average pore size of the single-tube ceramic membranes used in ceramic membrane contactor is miscellaneous on the outside of film for 50nm
Alcohol is flowed, in Bottomhole pressure ethylenediamine and the isooctane by weight 1 of film:90 solution prepared make after absorption
H in fusel2S, which is reduced to, to be not detected, and obtained fusel more solito detaches component, can recycle.
The preparation of 5 sunglo iron orchid fermentate of embodiment
A certain amount of wine yeast VL1 is taken, PDA culture medium is seeded to(By peeling potatoes, it is cut into about 2cm2Fritter, put
Enter and boil 30min in the beaker of 1500mL, then filtered with double gauze, takes its filtrate to add glucose, then complement to 1000mL)
In, it carries out strain and spreads cultivation, obtain yeast liquid.
It after the blade of sunglo iron orchid is cleaned, takes 1Kg to be ground into wet powder, adds into 15Kg deionized waters, be added
110g glucose adds 250mL yeast bacteria liquid as carbon source, 120g peptones as nitrogen source, with sodium bicarbonate adjust pH to
After 4.0~4.5 or so, in 28~30 DEG C in 30 h of fermentation cylinder for fermentation.The obtained supernatant that ferments passes through quartz filter
It after being filtered, is re-fed into the purpose ceramic-film filter of 50 μm of average pore size and is filtered except thalline, during ceramic membrane filter
Pressure 0.15MPa, ceramic membrane filtrate is when 45~50 DEG C are concentrated under reduced pressure into volume and are reduced to 1/3, then carries out at 80~85 DEG C
Vacuum drying, obtains sunglo iron orchid fermentate.
The preparation of 6 sunglo iron orchid fermentate of embodiment
A certain amount of wine yeast VL1 is taken, PDA culture medium is seeded to(By peeling potatoes, it is cut into about 2cm2Fritter, put
Enter and boil 30min in the beaker of 1500mL, then filtered with double gauze, takes its filtrate to add glucose, then complement to 1000mL)
In, it carries out strain and spreads cultivation, obtain yeast liquid.
It after the blade of sunglo iron orchid is cleaned, takes 1Kg to be ground into wet powder, adds into 12Kg deionized waters, be added
90g glucose adds 200mL yeast bacteria liquid as carbon source, 100g peptones as nitrogen source, with sodium bicarbonate adjust pH to
After 4.0~4.5 or so, in 30~32 DEG C in 40 h of fermentation cylinder for fermentation.The obtained supernatant that ferments passes through quartz filter
It after being filtered, is re-fed into the purpose ceramic-film filter of 200 μm of average pore size and is filtered except thalline, during ceramic membrane filter
Pressure 0.20MPa, ceramic membrane filtrate is when 45~50 DEG C are concentrated under reduced pressure into volume and are reduced to 1/3, then carries out at 80~85 DEG C
Vacuum drying, obtains sunglo iron orchid fermentate.
The preparation of 7 sunglo iron orchid fermentate of embodiment
A certain amount of wine yeast VL1 is taken, PDA culture medium is seeded to(By peeling potatoes, it is cut into about 2cm2Fritter, put
Enter and boil 30min in the beaker of 1500mL, then filtered with double gauze, takes its filtrate to add glucose, then complement to 1000mL)
In, it carries out strain and spreads cultivation, obtain yeast liquid.
It after the blade of sunglo iron orchid is cleaned, takes 1Kg to be ground into wet powder, adds into 20Kg deionized waters, be added
130g glucose adds 200mL yeast bacteria liquid as carbon source, 100g peptones as nitrogen source, with sodium bicarbonate adjust pH to
After 4.0~4.5 or so, in 26~28 DEG C in 35 h of fermentation cylinder for fermentation.The obtained supernatant that ferments passes through quartz filter
It after being filtered, is re-fed into the purpose ceramic-film filter of 200 μm of average pore size and is filtered except thalline, during ceramic membrane filter
Pressure 0.30MPa, ceramic membrane filtrate is when 45~50 DEG C are concentrated under reduced pressure into volume and are reduced to 1/3, then carries out at 80~85 DEG C
Vacuum drying, obtains sunglo iron orchid fermentate.
The liposomal of 8 sunglo iron orchid fermentate of embodiment
Soybean lecithin 50g, cholesterol 15g, vitamin E 0.5g, Tween 80 0.6g, sorbester p17 0.4g is taken to be uniformly mixed, then
It is scattered in the ether of 280mL, adds 2g polyethylene of dispersing agent alcohol 400, be uniformly mixed under ultrasonication and be used as oil phase;It will
Sunglo iron orchid fermentate is dissolved according to concentration 5wt% in deionized water, as water phase, then by 40mL under the action of ultrasound
Water phase be added dropwise in oil phase, then vacuum distillation removes ether at 30 DEG C, then 500ml is added in the obtained gel
The phosphate buffer solution of pH6.8, being evaporated under reduced pressure at 30 DEG C makes volume be reduced to original 1/4, using supersound process
Afterwards, liposome turbid liquor is obtained.
The TEM photos of liposome turbid liquor are as shown in Figure 1, it can be seen from the figure that liposomal particle size is evenly distributed, encapsulating
Property is good.The particle diameter distribution of suspension is as shown in Fig. 2, it is seen that the average grain diameter of liposome is slightly larger than 100 μm.
Liposome turbid liquor 5.0mL is taken, is placed in ultra-filtration centrifuge tube(Molecular cut off 100kDa), in 2500rpm rotating speeds
Lower centrifugation 15min, filtrate use the content of colorimetric method for determining glycine betaine, are calculate by the following formula encapsulation rate:
Encapsulation rate=(The amount of betaine in amount of betaine-filtrate in water phase)Amount of betaine × 100% in/water phase
The encapsulation rate being calculated is 73.4%.As can be seen that above-mentioned method can preferably carry out sunglo iron orchid fermentate
Encapsulating.
The liposomal of 9 sunglo iron orchid fermentate of embodiment
Soybean lecithin 60g, cholesterol 20g, vitamin E 0.8g, Tween 80 0.8g, sorbester p17 0.9g is taken to be uniformly mixed, then
It is scattered in the ether of 320mL, adds 1.5g polyethylene of dispersing agent alcohol 400, be uniformly mixed under ultrasonication and be used as oil phase;
Sunglo iron orchid fermentate is dissolved according to concentration 4wt% in deionized water, it, then will under the action of ultrasound as water phase
The water phase of 50mL is added dropwise in oil phase, then vacuum distillation removes ether at 35 DEG C, then is added 600ml's in obtained gel
The phosphate buffer solution of pH6.8, being evaporated under reduced pressure at 32 DEG C makes volume be reduced to original 1/4, using supersound process
Afterwards, liposome turbid liquor, liposome encapsulation 70.8% are obtained.
The liposomal of 10 sunglo iron orchid fermentate of embodiment
Soybean lecithin 45g, cholesterol 13g, vitamin E 0.4g, Tween 80 0.7g, sorbester p17 0.5g is taken to be uniformly mixed, then
It is scattered in the ether of 300mL, adds 2.5g polyethylene of dispersing agent alcohol 400, be uniformly mixed under ultrasonication and be used as oil phase;
Sunglo iron orchid fermentate is dissolved according to concentration 6wt% in deionized water, it, then will under the action of ultrasound as water phase
The water phase of 50mL is added dropwise in oil phase, then vacuum distillation removes ether at 30 DEG C, then is added 400ml's in obtained gel
The phosphate buffer solution of pH6.8, being evaporated under reduced pressure at 30 DEG C makes volume be reduced to original 1/4, using supersound process
Afterwards, liposome turbid liquor, liposome encapsulation 69.4% are obtained.
The preparation of 11 facial mask of embodiment
In the deionized water of 50g, moisturizer glycerine 8g and solubilizer Cremophor RH40 0.2g is added, is warming up to 60 DEG C
30min is kept, and is stirred evenly;Then, it continuously adds and takes aloe extract 0.2g, Chinese gooseberry fermentate 0.25g, sunglo
Liposome 0.15g, preservative methylisothiazolinone 0.1g, essence 0.05g, the citric acid 0.05g of iron orchid fermentate, stirring are equal
After even, add water 50g, after stirring evenly, obtain facial mask liquid, facial mask liquid is infiltrated on again on facial mask special non-woven fabric, then carry out nothing
Bacterium bag dress to get.
The preparation of 12 facial mask of embodiment
In the deionized water of 60g, moisturizer glycerine 9g and solubilizer Cremophor RH40 0.4g is added, is warming up to 65 DEG C
40min is kept, and is stirred evenly;Then, it continuously adds and takes aloe extract 0.3g, Chinese gooseberry fermentate 0.20g, sunglo
Liposome 0.12g, preservative methylisothiazolinone 0.2g, essence 0.07g, the citric acid 0.04g of iron orchid fermentate, stirring are equal
After even, add water 55g, after stirring evenly, obtain facial mask liquid, facial mask liquid is infiltrated on again on facial mask special non-woven fabric, then carry out nothing
Bacterium bag dress to get.
The preparation of 13 facial mask of embodiment
In the deionized water of 45g, moisturizer glycerine 5g and solubilizer Cremophor RH40 0.15g is added, is warming up to 55
DEG C 25min is kept, and stirred evenly;Then, it continuously adds and takes aloe extract 0.3g, Chinese gooseberry fermentate 0.4g, pine
Liposome 0.2g, preservative methylisothiazolinone 0.15g, essence 0.1g, the citric acid 0.1g of trailing plants iron orchid fermentate, stirring are equal
After even, add water 60g, after stirring evenly, obtain facial mask liquid, facial mask liquid is infiltrated on again on facial mask special non-woven fabric, then carry out nothing
Bacterium bag dress to get.
Tyrosinase activity inhibits experiment
The magnificent macaque Fructus Persicae fermented product obtained in embodiment 2 is dissolved according to the concentration of 10wt% in deionized water, according to following table
6 test systems are prepared, Chinese gooseberry fermentate solution is used wherein in T1 and T2 solution.
As a contrast, it is compared using the direct extract of Chinese gooseberry fermentate, the preparation process of direct extract
It is:Chinese gooseberry is removed the peel, takes the pulp of 1Kg to break into pulp, then pulp is added into 10Kg deionized waters, in 60 DEG C of items
Heating extraction 0.5h under part, supernatant are re-fed into the ceramic membrane of 50 μm of average pore size after quartz filter is filtered
It is filtered in filter, pressure 0.2MPa during ceramic membrane filter, ceramic membrane filtrate is concentrated under reduced pressure into body in 45~50 DEG C
Product is when being reduced to 1/3, then carries out the vacuum drying at 80~85 DEG C, obtains Chinese gooseberry water extract, by water extract according to
The concentration of 10wt% is dissolved in deionized water.Chinese gooseberry water extract is used in 2 solution of T ' 1 and T '.
C2 pipes are put in heating water bath 10min in 37 DEG C of water-baths, are returned to zero under wavelength 475nm, C1 pipes are put in 37 DEG C of water
After bathing 10min, add 1mL100U/mL tyrosinase diphenolases, continue water-bath 10min, measures C1 pipe absorbance values;Same method,
It is returned to zero with T2 and measures T1 absorbance values;Maximum inhibition A of the sample to tyrosinase diphenolase is calculated as follows.
Inhibiting rate A=(ODC1-ODT1)/ ODC1×100%
According to the maximum inhibition to tyrosinase diphenolase being measured in the same method to water extract, and using 1% ursin as sample
Control.
Obtained inhibiting rate is as shown in the table:
DPPH radicals scavengings are tested
According to " tyrosinase activity inhibits experiment ", the 2 × 10 of 10mL Chinese gooseberry fermentate solution and 10mL are taken-4mol/L
DPPH solution mixings(A1 is managed);Take 10mL deionized water and 10mL 2 × 10-4The DPPH solution mixings of mol/L(A2 is managed);
Take the absolute ethyl alcohol and 10mL Chinese gooseberry fermentate solution mixings of 10mL(A3 is managed);After reacting 30min, surveyed at 517nm
The absorbance value of A1, A2, A3 pipe, is denoted as ODA1, ODA2 and ODA3 respectively, and clearance rate is calculated as follows.
DPPH clearance rates=([ODA2+ODA3)-ODA1]/ODA2×100%
According to the DPPH free radical scavenging activities for being measured in the same method Chinese gooseberry water extract.
According to the fermentate solution and water extract solution of same method sequentially determining a concentration of 0.5%, 1%, 4%, 6%, 8% concentration
DPPH free radical scavenging activities calculate IC50 values.As shown in the table:
As can be seen from the above table, Chinese gooseberry fermentate has the effect of preferably removing DPPH free radicals, through everfermentation it
Afterwards, active ingredient can be made by further decomposing, removal efficiency is better than the extract of direct water extraction.
Moistening effect is tested
In a sealed container, the ammonium sulfate and solution of potassium carbonate for placing saturation respectively, it is 80% and 40% to form relative humidity
Environment, then place glass slide in a reservoir, the sunglo being prepared in 5 drop embodiments 8 be added dropwise on the surface of glass slide respectively
The aqueous solution of sunglo iron orchid fermentate 10wt%, is calculated as follows in iron orchid fermentate liposome turbid liquor and embodiment 5:
Moisturizing rate=M2/M1 × 100%
M2 is biodiversity after placing, g;M1 is biodiversity before placing, g;
Moisturizing rate is measured respectively at the 2nd, 4,6,8,10h, the results are shown in table below:
As can be seen from the table, can obviously have better moistening effect using trailing plants iron orchid liposome turbid liquor.
The skin irritation test of facial mask
Using new zealand white rabbit, before carrying out acute skin irritation test, rabbit backbone diamond wool is cut, unhairing range
Left and right Ge Yue 6cm2, formally when experiment, facial mask is cut to 2.5 × 2.5 cm2Square be overlying on left side skin on, then use
Two layers of gauze and one layer of glassine paper covering, then fixed with nonirritant adhesive plaster and bandage, it is applied with time 4h, right side skin is made
For control, after the test, skin removed residue is cleaned with warm water, the reaction of tested skin is observed respectively at 1,24,48h,
Using《Cosmetics health specification》Carry out skin wound repair scoring.As a result it shows as follows:
As can be seen from the above table, facial mask of the invention is non-stimulated to skin.