CN108635324B - Antioxidant, preparation method and application thereof, antioxidant additive and preparation method thereof - Google Patents
Antioxidant, preparation method and application thereof, antioxidant additive and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of antioxidants, and provides an antioxidant, a preparation method and application thereof, an antioxidant additive and a preparation method thereof. A method for preparing antioxidant comprises extracting algae plant with supercritical or subcritical extraction to obtain extractive solution, and desolventizing the extractive solution under reduced pressure to obtain the desired antioxidant. An antioxidant is prepared by the preparation method of the antioxidant. The invention also provides an application of the antioxidant, and the antioxidant is used in food, health care products or cosmetics. The invention also provides a preparation method of the antioxidant additive, wherein the antioxidant is provided and is separated and purified to obtain an algae extract, and the algae extract is dissolved in grease to obtain the required antioxidant additive. An antioxidant additive is prepared by the preparation method of the antioxidant.
Description
Technical Field
The invention relates to the technical field of antioxidants, and particularly relates to an antioxidant, a preparation method and application thereof, an antioxidant additive and a preparation method thereof.
Background
At present, the antioxidants for cosmetics generally use food-grade antioxidants. Food-grade antioxidants are mainly classified into two types, one is an oil-soluble antioxidant mainly used for plants, and the other is a water-soluble antioxidant mainly used for sausages. The shelf life of food grade vegetable oils is typically one and a half years. The sausage products have shorter shelf life and generally need to be stored in a cold storage in the storage process.
The shelf life of the cosmetics is more than three years, the cosmetics are stored at room temperature, and the oil-water mixture is more, so that the risk that the cosmetics are easily oxidized is increased. Therefore, the current food-grade antioxidant hardly meets the requirements of cosmetics.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an antioxidant, a preparation method and application thereof, an antioxidant additive and a preparation method thereof.
In order to solve the technical problems, the invention adopts the following technical scheme. A method for preparing antioxidant comprises extracting algae plant with supercritical or subcritical extraction to obtain extractive solution, and desolventizing the extractive solution under reduced pressure to obtain the desired antioxidant.
Preferably, the extraction liquid obtained by extracting the algae with supercritical extraction or subcritical extraction is obtained by preparing algae powder from the algae, soaking the algae powder in a soaking liquid, and extracting with supercritical extraction or subcritical extraction to obtain the extraction liquid, wherein the soaking liquid comprises vegetable oil and a penetrating agent.
Preferably, the particle size of the algae powder is 2-150mm, the soaking time is 10min-5h, and the soaking temperature is 35-85 ℃.
Preferably, the weight ratio of the soak solution to the algae powder is 1-5: 1; the weight ratio of the vegetable oil to the penetrant is 1-100: 1.
preferably, the vegetable oil is one or a combination of several of sunflower oil, macadamia nut oil, jojoba oil, rose hip oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil, camellia oil or shea butter; the penetrating agent is one or more of tocopherol, Olibanum extract, herba Rosmarini officinalis extract, paeonol, and food-grade chemically synthesized antioxidant.
Preferably, the algal plant is an alga selected from the group consisting of Austenophyceae, Spirulina pennata, Ascophyllum nodosum, Fucus serratus, Coccocus acutus, Zostera marina, Megassum amabilis, Sargassum pallidum, Ascophyllum Nodosum, Ascophyllum Nostoc, Ascophyllum Nostoides, Ascophyllum Nostolonicum, Ascophyllum Nostoloniferum tenuissimum, Chlorella minutissimulatum, Ascophyllum Nostoloniferum, Ascophyllum, One or more of Scopulariopsis Fulviformis and Porphyridium sp.
An antioxidant is prepared by the preparation method of the antioxidant.
An application of the antioxidant is that the antioxidant is used in food, health care products or cosmetics.
A preparation method of an antioxidant additive comprises providing the antioxidant, separating and purifying to obtain algae extract, and dissolving the algae extract in oil to obtain the desired antioxidant additive.
An antioxidant additive, characterized in that: the antioxidant is prepared by the preparation method of the antioxidant.
The invention has the beneficial effects that: the method is simple and feasible, and because the raw materials of the algae plants are natural traditional edible plants, the raw materials are rich and cheap, and the obtained product is free of pollution, toxic and side effects, safe and practical, the method has strong industrial practicability and operability.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood by those skilled in the art, the present invention is further described with reference to the following embodiments.
The invention provides a preparation method of an antioxidant, which adopts supercritical extraction or subcritical extraction to extract an extract liquor from an algae plant, and the required antioxidant can be obtained after the obtained extract liquor is decompressed and desolventized.
Preferably, the extraction of the algae extract from the algae plants by the supercritical extraction or the subcritical extraction comprises the steps of preparing the algae plants into algae powder, soaking the algae powder in a soaking solution, and extracting by the supercritical extraction or the subcritical extraction to obtain the algae extract, wherein the soaking solution comprises vegetable oil and a penetrating agent.
Wherein, preferably, the algae is Austenophyta, Bonnate Spirulina, Porphyridium, Fucus vesiculosus, Codium acutillus, Zostera marina, Megassum amabilis, Blastoma sp, Padina pavonica, Chlorella pumila, Chlorella saxatilis, Cladosiphon rufimbriatum, Rhodophyta, Spirulina maxima, Chlorella aurora, Macrocystis, Cladosporium, Aphyllum japonicum, Aphyllophora, Rhodophyta bluish, Spirulina, Chlorella, Polysiphonia lansii, Fucus Antarctica, Pleurospora pulcherrima, Ascophyllum, Chlamydomonas, Rhodophyta helminthospermum, Phaeodactylum, Pteridophyllum tricornutum, Codium tsugae, Ascophyllum elongatum, Microdocladium, Euglena tenuis, Chlorella, Sargassum, Euglena lanciformis, Euglena, Dunaliella salina, Eu, One or more of Skeletonema costatum, Alariaceae, and Porphyridium. Most preferably, the algal plant is Haematococcus pluvialis.
The algae powder is prepared by removing impurities from algae, pulverizing with pulverizer, and sieving. Preferably, the powder particle size of the algae powder is 2-150mm, more preferably, the powder particle size of the algae powder is 10-120mm, and most preferably 90 mm. The soaking of the algae powder in the soaking solution can be carried out by adding the algae powder into a soaking tank, and injecting vegetable oil and penetrant.
Preferably, the vegetable oil is one or a combination of several of sunflower oil, macadamia nut oil, jojoba oil, rose hip oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil, camellia oil or shea butter. Can effectively extract antioxidant from algae. More preferably, the vegetable oil is one or more of sunflower oil, macadamia nut oil and jojoba oil. Most preferably, the vegetable oil is sunflower oil, which has a better extraction effect.
Preferably, the penetrating agent is one or more of tocopherol, frankincense extract, rosemary extract, paeonol and food-grade chemically synthesized antioxidant. Can effectively extract antioxidant from algae. More preferably, the penetrating agent is one or more of tocopherol, frankincense extract and chemically synthesized antioxidant. Most preferably, the penetrating agent is a frankincense extract, and the extraction effect is better.
Preferably, the weight ratio of the soak solution to the algae powder is 1-5: 1, the product can be effectively extracted. Preferably, the weight ratio of the soak solution to the algae powder is 1-4: 1, optimally, the weight ratio of the soak solution to the algae powder is 3.5: 1.
preferably, the weight ratio of the vegetable oil to the penetrant is 1-100: 1, the product can be effectively extracted. More preferably, the weight ratio of the vegetable oil to the penetrant is 10-80: 1, optimally the weight ratio of the vegetable oil to the penetrant is 50: 1.
generally, the difference in the soaking temperature is large depending on the properties of the raw material itself. In the invention, preferably, the soaking temperature is 35-85 ℃, and the extraction effect is good. More preferably, the soaking temperature for soaking is 45-75 ℃, and most preferably, the soaking temperature for soaking is 65 ℃. Preferably, the soaking time is 10min-5h, the soaking time is preferably 20min-3.5h, and most preferably 3 h.
The specific steps of supercritical extraction or subcritical extraction are as follows: extracting with supercritical carbon dioxide extraction unit at 30-75 deg.C under 1.5-90 kg pressure with carbon dioxide as extractant, and performing supercritical or subcritical extraction on the soaked algae powder. The pressure is preferably 5-80 kg, most preferably 50 kg. The extraction temperature is more preferably 45 to 75 ℃ and most preferably 65 ℃.
The yields of antioxidant obtained for different weight ratios of vegetable oil to algal flour, different penetrants, different extraction temperatures and different algal species are described below.
1. Effect of the weight ratio of vegetable oil algae powder on the yield of the obtained antioxidant
The process conditions are as follows: the extraction pressure is 50 kg, the particle size of algae powder is 70mm, the algae is haematococcus pluvialis, the soaking time is 2 hours, the soaking temperature is 75 ℃, the penetrant is a chemically synthesized food antioxidant, and the weight ratio of the penetrant to the vegetable oil is 1: 30. the results are shown in Table 1.
TABLE 1 yield of antioxidant obtained when extracting with different weight ratios of vegetable oil algae powder
| Vegetable oil | The weight ratio of the vegetable oil to the algae powder | Yield of antioxidant (%) |
| Jojoba oil | 3.5:1 | 5.8 |
| Rose fruit oil | 3.5:1 | 0.2 |
| Avocado oil | 3.5:1 | 10.2 |
| Macadamia nut oil | 3.5:1 | 6.4 |
| Sweet almond oil | 3.5:1 | 11.7 |
| Shea butter | 3.5:1 | 1.8 |
| Soybean oil | 3.5:1 | 9.9 |
| Wheat germ oil | 3.5:1 | 12.3 |
| Camellia oil | 3.5:1 | 7.9 |
| Sunflower oil | 3.5:1 | 14.8 |
As shown in table 1, the yield of antioxidant was the highest when sunflower oil was used as the vegetable oil.
2. Effect of penetrant on the yield of antioxidant obtained
The process conditions are as follows: the weight ratio of the vegetable oil to the algae powder is 3: 1, the vegetable oil is soybean oil, the crushed algae powder has the particle size of 70mm, the soaking time is 2.5 hours, the extraction pressure is 70 kilograms, and the extraction temperature is 50 ℃. The results are shown in Table 2.
TABLE 2 yield of antioxidant obtained by extraction with different penetrants
| Penetrant | Weight ratio of penetrant to vegetable oil | Yield of antioxidant (%) |
| Boswellia extracts | 1：4 | 14.2 |
| Rosemary extract | 1：4 | 9.8 |
| Tocopherol | 1：4 | 6.6 |
| Paeonol | 1：40 | 8.8 |
| TT | 1：40 | 6.5 |
| BHA | 1：40 | 7.2 |
| TBHQ | 1：40 | 9.6 |
As shown in Table 2, the yield of the antioxidant was the highest when the extract of Olibanum was used as the penetrant.
3. Effect of extraction temperature on the yield of antioxidant obtained
The process conditions are as follows: the weight ratio of the vegetable oil to the algae powder is 3: 1, crushing the algae powder into particles with the particle size of 70mm, soaking for 3 hours, extracting under the pressure of 70 kg, using a penetrating agent which is tocopherol, wherein the weight ratio of the penetrating agent to the vegetable oil is 1: 4. the results are shown in Table 3.
TABLE 3 yields of antioxidant obtained when extraction is carried out at different extraction temperatures
| Extraction temperature (. degree.C.) | The weight ratio of the sunflower oil to the chlorella raw material | Yield of antioxidant (%) |
| 30 | 3:1 | 3.5 |
| 35 | 3:1 | 4.2 |
| 40 | 3:1 | 7.8 |
| 45 | 3:1 | 9.0 |
| 50 | 3:1 | 11.5 |
| 55 | 3:1 | 11.9 |
| 60 | 3:1 | 13.5 |
| 65 | 3:1 | 14.8 |
| 70 | 3:1 | 13.0 |
| 75 | 3:1 | 9.8 |
| 80 | 3:1 | 7.0 |
| 85 | 3:1 | 6.2 |
As can be seen from Table 3, the antioxidant yield was high at an extraction temperature of 45-75 ℃ and was highest at an extraction temperature of 65 ℃.
4. Effect of algae on the yield of antioxidants obtained
The process conditions are as follows: the extraction pressure is 50 kg, the particle size of the algae powder is 55mm, the soaking time is 4.5 hours, the soaking temperature is 75 ℃, the penetrant is an frankincense extract, and the ratio of the penetrant to the vegetable oil is 1: 30. the results are shown in Table 4.
TABLE 4 yields of antioxidants obtained with different algae species extraction
As can be seen from Table 4, when the algae species are Codium spinulosum, Zostera marina, Megasphaera nigra, Sargassum blunt, Spirulina, Pleurosperma, Cystolochia pulcherrima, Rhodophyta helminthica, Pinus tomentosa, Phaeodactylum tricornutum, Pteridium tsugae, Codium corallinum, Haematococcus pluvialis, Kappaphycus alvarezii, Cladosporium palmatum, Porphyromonas purpurea, the yield of the antioxidant is high; the yield of antioxidant is highest when the algae species is haematococcus pluvialis.
The invention also provides an antioxidant which is prepared by the preparation method of the antioxidant. GC-MS analysis is carried out on the antioxidant prepared by the method, the linoleic acid content in the antioxidant is more than or equal to 30 percent, the linolenic acid content is more than or equal to 50 percent, the content of the linolenic acid is more than or equal to 18 percent of the eicosa unsaturated fatty acid, the vitamin C content is more than or equal to 0.02 percent, the tocopherol content is more than or equal to 0.005 percent, the vitamin A content is more than or equal to 0.0008 percent, and the amino acid content.
The invention also provides an application of the antioxidant, and the antioxidant is used in food, health care products or cosmetics. The antioxidant can be used as food or health product for direct eating after refining, or as additive added into food, health product or cosmetic to make it have effects of resisting oxidation and scavenging free radicals.
The invention also provides a preparation method of the antioxidant additive, wherein the antioxidant is provided and is separated and purified to obtain an algae extract, and the algae extract is dissolved in grease to obtain the required antioxidant additive. The oil is one or more of sunflower seed oil, macadamia nut oil, jojoba oil, rose hip oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil, camellia oil, shea butter, lard, beef tallow, horse oil, snake oil, sheep oil and fish oil.
The invention also provides an antioxidant additive, which is prepared by the preparation method of the antioxidant additive.
Specific examples are provided below
Preparation of antioxidant
Example 1
Extracting the soaked algae powder by adopting an intermittent supercritical extraction tank set: 100 g of crushed algae powder is taken as a raw material, the crushed algae powder with the particle size of 20mm is added into a leaching tank, 400 g of soaked sunflower seed oil and 2 g of penetrating agent frankincense extract are injected, the soaking time is 3h, and the soaking temperature is 75 ℃. After leaching, the soaked matter in the leaching tank is subjected to supercritical extraction, 410.6 g of the obtained alga antioxidant mixture is subjected to decompression and desolventization, 8.6 g of alga oil mixture is obtained, and the yield is 8.6%. The algae powder is Asterina asteroides.
Example 2
Extracting the soaked algae powder by adopting an intermittent supercritical extraction tank set: 100 g of crushed algae powder is taken as a raw material, the crushed particle size is 90mm, the crushed algae powder is added into a leaching tank, 200 g of camellia oil and 5 g of penetrating agent tocopherol are injected and soaked for 20min, and the soaking temperature is 30 ℃; after leaching, the soaked matter in the leaching tank is subjected to supercritical extraction, 214.0 g of the obtained alga antioxidant mixture is subjected to pressure reduction and desolventization, 9 g of alga oil mixture is obtained, and the yield is 9%. The algae powder is red leaf algae.
Example 3
Extracting the soaked algae powder by adopting an intermittent supercritical extraction tank set: 100 g of crushed algae powder is taken as a raw material, the crushed particle size is 20mm, the crushed algae powder is added into a leaching tank, 300 g of macadamia nut oil and 2 g of penetrant synthetic antioxidant are injected, the soaking time is 3 hours, and the soaking temperature is 75 ℃. After leaching, the soaked matter in the leaching tank is subjected to supercritical extraction, 310.8 g of the obtained alga antioxidant mixture is subjected to decompression and desolventization, 8.8 g of alga oil mixture is obtained, and the yield is 8.8%. The algae powder is Haematococcus pluvialis.
Example 4
Extracting the soaked algae powder by adopting an intermittent supercritical extraction tank set: 100 g of crushed algae powder is taken as a raw material, the crushed particle size is 50mm, the crushed algae powder is added into a leaching tank, 200 g of camellia oil and 5 g of penetrating agent paeonol are injected and soaked for 120min, and the soaking temperature is 76 ℃; after leaching, the soaked matter in the leaching tank is subjected to supercritical extraction, 214.0 g of the obtained alga antioxidant mixture is subjected to pressure reduction and desolventization, 9 g of alga oil mixture is obtained, and the yield is 9%. The algae powder is austenite algae.
Examples 5 to 39
The process adopts an intermittent supercritical extraction tank set to extract the soaked algae powder: 100 g of crushed algae powder is used as a raw material, the crushed algae powder with the particle size of 20mm is added into a leaching tank, 400 g of sunflower seed oil is soaked in the leaching tank, the pressure adopted by the extraction method is 50 kg, the particle size of the algae powder is 55mm, the soaking time is 4.5 hours, the soaking temperature is 75 ℃, the penetrant is a frankincense extract, and the ratio of the penetrant to the vegetable oil is 1: 30.
examples 5 to 39 differ in the species of algae used, which are, respectively, Bonnate Spirulina, Isaria stramenorrhiza, Fucus serratus, Codium crassifolia, Zostera marina, Macrobrachium nipponensis, Sargassum thunbergii, Fucus pulmonarius, Chlorella pumila, Cladosiphon hainanensis, Rhodophyta, Spirulina maxima, Chlorella infirmidis, Macrobrachium roseum, Cladosporium herbarum, Aphyllum portulare, Rhodophyta, Spirulina platensis, Chlorella, Polysiphonia lanuginosa, Fucus euphorbia, Asclepias lancifolia, Chlamydomonas Lanceolata, Rhodophyta, Phaeodactylum tricornutum, Aphis hirsutum, Ascophyllum elongatum, Microlaevigatum, Gymnodinaria, Euglena, Chlorella minuta, Euglena, Eugle, Alariapteris Functoria, Porphyridium sp. The yields (% in units) obtained are shown in Table 4, and are not described in detail herein.
Second, antioxidant Property study of antioxidant
Examples 40 to 55
The antioxidants obtained in examples 1 to 39 were mixed, and 0.2 g of the mixed antioxidant complex and 99.8 g of oil and fat were taken. The oil and fat are sunflower seed oil, macadamia nut oil, jojoba oil, rose hip oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil, camellia oil, shea butter, lard, beef tallow, horse oil, snake oil, sheep oil and fish oil. The oxidation stability tester for oil production Switzerland grease tests the oxidation stability time at 110 ℃ of the oil production Switzerland grease with and without the compound antioxidant:
TABLE 5 antioxidant stabilization time of respective oils without and with Complex antioxidant
Examples 56 to 78
The antioxidant performance of the antioxidant of single algae and the antioxidant of the composite algae are researched, and the experimental method is the same as that of the embodiment 40-55.
The single algae is selected from Haematococcus pluvialis, Chlorella pumila, Chlorella sanguinea, Chlorella sp. Examples 56-61 were obtained.
The composite algae is selected from Austenophyceae, Bonnate Spirulina, Ascophyllum nodosum, Fucus serratus, Coccomys spinosus, Zostera marina, Megassum pallidum, Blastoma algae, Padina pavonica, Chlorella pumila, Cladosiphon okamurensis, Rhodophyta, Spirulina maxima, Chlorella miniata, Macrocystis, Cladosiphonium, Aphyllophora, Rhodophyta bluish, Spirulina, Chlorella, Polysiphonia lansium, Fucus vesiculosus, Ascophyllum nodosum, Ascophyllum, Microlaematococcus, Microlaena, Coccomys tenuiflora, Chlorella, Microphyllocladium, Euglena, Coccoides, Coccomyxoides, Ascophyllum perus, Coccoides, Coccomyxoides, Euglena, Coccomyxoides, Coccocus effusum perus, Coccoides, Coccocus, Coccomyxoides, Coccocus, Coccomyxoides, Cocco, 3-5 kinds of algal oil mixture are randomly selected from porphyridium to be compounded to obtain the examples 62-78.
All the antioxidants were added in an amount of 0.2 g, the oil was 99.8 g, and the oil was rosehip oil.
TABLE 6 antioxidant stabilization time of antioxidant of single algae and antioxidant of composite algae with addition of antioxidant of single algae and composite algae
Preparation of antioxidant additive and research of antioxidant performance
Examples 79 to 94
Respectively separating and purifying the antioxidants obtained in the embodiments 1 to 39 to obtain algae extracts, mixing the algae extracts to obtain a composite antioxidant additive, and dissolving 2 g of the mixed composite antioxidant additive and 98 g of grease to obtain the antioxidant additive. The oil and fat are sunflower seed oil, macadamia nut oil, jojoba oil, rose hip oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil, camellia oil, shea butter, lard, beef tallow, horse oil, snake oil, sheep oil and fish oil. Adopting a Switzerland grease oxidation stability tester to test the oxidation stability time at 110 ℃ with and without an antioxidant:
TABLE 7 antioxidant stabilization time of oils without and with Complex antioxidant additives
Examples 95 to 117
The antioxidants obtained in examples 56 to 78 were respectively separated and purified to obtain algae extracts, and 0.2 g of the algae extracts were taken, the oil and fat was 99.8 g, and the oil and fat was rosehip oil. That is, examples 95 to 100 are antioxidant additives of individual algae, which are Haematococcus pluvialis, Chlorella pumila, Chlorella sanguinea, Haematococcus sp, Spirulina sp, Chlorella sp. Example 101-, Selecting 3-5 kinds of algae oil mixture from Palmaria palmata, Skeletonema costatum, Alariaceae, and Porphyromonas. The antioxidant stabilization time results are shown in table 8.
TABLE 8 antioxidant stabilization time of antioxidant additives of single algae and antioxidant additives of composite algae
Fourth, application of antioxidant additive in cosmetics
Example 118-
In the conventional cosmetic formula, the content of the vegetable oil is 0.5-100%, the content of the antioxidant additive is 0.05%, the antioxidant stabilization time at 110 ℃ of the antioxidant additive and the antioxidant additive is tested by adopting a Switzerland grease oxidation stability tester, wherein the embodiment 128-133 is the composite vegetable oil, the vegetable oil is the vegetable oil which is allowed to be used by the cosmetic, the compounding proportion is that the content of the simple grease is randomly distributed and is 40 g, and the content of the composite grease is 100 g. The antioxidant stabilization time results are shown in table 9.
TABLE 9 antioxidant stabilization time without and with antioxidant additives
Claims (4)
1. A preparation method of an antioxidant is characterized by comprising the following steps: extracting an extract from the algae by supercritical extraction or subcritical extraction, and performing pressure reduction and desolventization on the extract to obtain the required antioxidant;
the extraction liquid obtained by extracting the algae plants by adopting the supercritical extraction or the subcritical extraction is that the algae plants are prepared into algae powder, then the algae powder is soaked in a soaking liquid, and the extraction liquid is obtained by utilizing the supercritical extraction or the subcritical extraction, wherein the soaking liquid consists of vegetable oil and a penetrating agent;
the particle size of the algae powder is 2-150mm, the soaking time is 10min-5h, and the soaking temperature is 35-85 ℃; the weight ratio of the soak solution to the algae powder is 1-5: 1; the weight ratio of the vegetable oil to the penetrant is 10-80: 1;
the vegetable oil is one or more of sunflower oil, macadamia nut oil, jojoba oil, sweet almond oil, avocado oil, wheat germ oil, soybean oil and camellia oil;
the penetrating agent is one or the combination of two of tocopherol and paeonol.
2. The method for producing an antioxidant according to claim 1, wherein: the algae is selected from Austenophyceae, Bonnaia spirulina, Ascophyllum, Fucus serratus, Coccocus acutus, Zostera marina, Megassum lansium, Megassum pallidum, Blastoma lugens, Padina pavonica, Chlorella pumila, Cladosiphon okamurensis, Rhodophyta, Spirulina maxima, Chlorella aurea, Macrocystis, Cladosiphon okamuranus, Aphyllophora, Rhodophyta robusta, Spirulina, Chlorella, Polysiphonia lansii, Fucus vesiculosus, Ascophyllum nodosum, Phaeophysalis, Coccocus, Ascophyllum perda, Coccolithospermum, Ascophyllum perda, Ascophyllum perus, Ascophyllum nodosum, Ascophyll, One or more of porphyridium.
3. An antioxidant, characterized by: the antioxidant is prepared by the method for preparing the antioxidant as claimed in claim 1.
4. The application of an antioxidant is characterized in that: use of the antioxidant according to claim 3 in food or cosmetics.
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