CN110257155A - The sub-critical extraction method and gained sea cucumber intestine egg oil of sea cucumber intestine egg oil - Google Patents
The sub-critical extraction method and gained sea cucumber intestine egg oil of sea cucumber intestine egg oil Download PDFInfo
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- 210000000936 intestine Anatomy 0.000 title claims abstract description 73
- 238000000605 extraction Methods 0.000 title claims abstract description 49
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 35
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 35
- 210000004681 ovum Anatomy 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 238000004108 freeze drying Methods 0.000 claims abstract description 15
- 239000003921 oil Substances 0.000 claims description 43
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000009874 alkali refining Methods 0.000 claims description 13
- 239000010779 crude oil Substances 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- NNPPMTNAJDCUHE-UHFFFAOYSA-N isobutane Chemical compound CC(C)C NNPPMTNAJDCUHE-UHFFFAOYSA-N 0.000 claims description 10
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 claims description 8
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical group CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- 239000001273 butane Substances 0.000 claims description 5
- 239000001282 iso-butane Substances 0.000 claims description 5
- 235000013847 iso-butane Nutrition 0.000 claims description 5
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 claims description 5
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 238000000638 solvent extraction Methods 0.000 claims description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 229910052740 iodine Inorganic materials 0.000 claims description 3
- 239000011630 iodine Substances 0.000 claims description 3
- 150000002978 peroxides Chemical class 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 claims description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 238000011161 development Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000004332 deodorization Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000001291 vacuum drying Methods 0.000 abstract 1
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 239000001294 propane Substances 0.000 description 4
- 238000000194 supercritical-fluid extraction Methods 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000258955 Echinodermata Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010016946 Food allergy Diseases 0.000 description 1
- 240000007058 Halophila ovalis Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 210000004126 nerve fiber Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000021081 unsaturated fats Nutrition 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Mechanical Engineering (AREA)
- Fats And Perfumes (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention proposes the sub-critical extraction method and gained sea cucumber intestine egg oil of a kind of sea cucumber intestine egg oil, belongs to field of biotechnology, the extracting method rational technology, green, low consumption and efficient, simple and safe operation.The technical solution include by raw material freeze-drying, crushing, subcritical abstraction, purification, vacuum drying and deodorization and etc. obtain sea cucumber intestine egg oil.The present invention can be applied to that the development and utilization of sea cucumber intestine ovum can be greatly speeded up in effective extraction of sea cucumber intestine egg oil.
Description
Technical field
The invention belongs to the sub-critical extraction method and gained of field of biotechnology more particularly to a kind of sea cucumber intestine egg oil seas
Join intestines egg oil.
Background technique
Sea cucumber belongs to Holothuroidea (Holothuroidea), is the ocean echinoderm to 8000 meters by the sea that lives, away from the present
Existing more than 600,000,000 years history, sea cucumber are food with seabed algae and planktonic organism.Sea cucumber is not only precious food and rare
Medicinal material.The nutritional ingredients such as phosphatide rich in, unsaturated fatty acid, have the elderly and women good in oil of sea cucumber
Meal supplement effect.However, traditional oil of sea cucumber is often due to containing histamine and bringing serious seafood allergy, so that largely
Crowd can not take.
Traditional oil of sea cucumber is after the extraction, it is difficult to which secondary operation uses.At the same time, sea cucumber intestine ovum is in process
Generally it is dropped or as fertilizers for potted flowers, brings serious waste.With going deep into for research, Holothurian machining byproduct is originally belonged to
Intestines ovum (internal organ and sexual gland, be also sea cucumber) it has been investigated that also various nutriments of sea cucumber rich in, such as contain
More rich Sea Cucumber Glycosides, a variety of sea cucumber polypeptides and a variety of amino acid and unsaturated fat sour component rich in, have
It improves immunity of organisms, inhibit tumour, anticoagulation, the physiological function for protecting nerve fiber.
Currently, the lipid component of various animals and plants materials, which extracts, usually all uses carbon dioxide supercritical extraction technique, but
The prior art is not registered the extraction process of sea cucumber intestine egg oil, it is main the possible reason is: on the one hand, carbon dioxide supercritical extraction
Equipment is expensive, too high with carbon dioxide supercritical extraction sea cucumber intestine egg oil cost, and investment is too big, for sea cucumber byproduct (sea cucumber
Intestines ovum) processing it is economically not cost-effective, on the other hand, it may be possible to because CO 2 supercritical process requirement under super-pressure into
Row operation, production safety have hidden danger, and production environment, personnel requirement are high.In view of existing for supercritical extraction technique at this stage kinds
Kind problem, it is difficult to be applied to large-scale production, therefore, seeking green, low consumption and efficient oil extraction novel technique becomes
Grease extracts the research hotspot in field in recent years.
Summary of the invention
The present invention proposes the sub-critical extraction method and its gained sea cucumber intestine egg oil of a kind of sea cucumber intestine egg oil, the extracting method
Rational technology, green, low consumption and efficient, simple and safe operation, this is particularly important effective extraction of sea cucumber intestine egg oil, can
To greatly speed up the development and utilization of sea cucumber intestine ovum.
In order to achieve the above object, the present invention provides a kind of sub-critical extraction method of sea cucumber intestine egg oil, including it is following
Step:
It chooses the sea cucumber intestine ovum raw material that pre-sifted is chosen to be freeze-dried, obtains freeze-drying sea cucumber intestine ovum raw material；
After the crushing of sea cucumber intestine ovum raw material will be lyophilized, it is placed in subcritical abstraction device and carries out solvent extraction, it will be molten after extraction
Agent and sea cucumber intestine ovum crude oil carry out separating treatment；
Phosphoric acid is added into sea cucumber intestine ovum crude oil and carries out degumming, sodium hydroxide solution is then added and is carried out under hot conditions
Alkali-refining deacidification after centrifugal treating, obtains alkali refining oil；
Alkali refining oil is dried and is deodorized under vacuum condition, sea cucumber intestine egg oil is obtained.
Preferably, the freeze-drying smashed partial size of sea cucumber intestine ovum raw material >=60 mesh.
Preferably, it is 30-55 DEG C that solvent-extracted condition, which is extraction temperature, extraction pressure is 0.2-1.0Mpa.
Preferably, solvent extraction solvent for use is at least one of ethane, propane, butane, iso-butane and dimethyl ether.
Preferably, being that separate pressure be 0.1- by the condition that solvent and sea cucumber intestine ovum crude oil carry out separating treatment
0.5Mpa, separation temperature are 20-65 DEG C.
Preferably, the phosphoric acid concentration being added is 85%, the Baume degrees of the sodium hydroxide solution of addition is 5-10 ° of B é, high
Warm condition is 80-90 DEG C, and the alkali refining time is 1-15s.
The sub-critical extraction method for the sea cucumber intestine egg oil as described in any of the above-described technical solution that the present invention also provides a kind of
Obtained sea cucumber intestine egg oil.
Preferably, acid value mgKOH/g < 10, the iodine number g/100g of the sea cucumber intestine egg oil are 70-150, peroxide value
G/100g < 2.
The present invention also provides a kind of cosmetics, sea cucumber intestine egg oil described in techniques described above scheme is main component.
The present invention also provides a kind of grease or beverage, sea cucumber intestine egg oil described in techniques described above scheme is main former
Material.
Compared with prior art, the advantages and positive effects of the present invention are:
1, sea cucumber intestine egg oil sub-critical extraction method provided by the invention uses ethane, propane, butane, iso-butane or diformazan
Ether not only can effectively avoid hot place using Freeze Drying Technique processing raw material as Extraction solvent, low boiling point, Yi Huishou
The problems such as managing bring raw material oxidation deterioration can also utmostly keep raw material freshness and nutritional ingredient to retain.
2, sea cucumber intestine egg oil sub-critical extraction method provided by the invention belongs to physical extraction technology, in extracting sea cucumber oil
Active constituent in oil of sea cucumber can be greatly kept not to be destroyed simultaneously, rational technology, green, low consumption and efficient, simple and safe operation,
This is particularly important effective extraction of sea cucumber intestine egg oil, can greatly speed up the development and utilization of sea cucumber intestine ovum.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
It chooses with the preferable sea cucumber intestine ovum raw material of batch integrality, it is desirable that raw material is fresh, free from admixture, free from extraneous odour etc..Sabot
It is freeze-dried after processing in -40 DEG C, obtains freeze-drying sea cucumber intestine ovum raw material；
Freeze-drying sea cucumber intestine ovum raw material is crushed using pulverizer, Particle size requirements >=60 mesh check cleanliness, such as after crushing
There are the foreign matters such as shell class, sea grass to remove in time；
It will be extracted in freeze-drying sea cucumber intestine ovum raw material merging subcritical abstraction device using ethane, extraction temperature 30
DEG C, extraction pressure is 0.2Mpa, by solvent and sea cucumber intestine ovum crude oil in separating pressure 0.1Mpa, at 20 DEG C of separation temperature after extraction
Carry out separating treatment；
The phosphoric acid that concentration 85% is added into sea cucumber intestine ovum crude oil carries out degumming, and the hydrogen-oxygen that Baume degrees is 5 ° of B é is then added
Change sodium solution and carries out alkali-refining deacidification 15s under 80 DEG C of hot conditions, after centrifugal treating, it is oily to obtain alkali refining；
Alkali refining oil is dried and is deodorized under 0.1-0.5Mpa vacuum condition, sea cucumber intestine egg oil is obtained.
Embodiment 2
It is identical as 1 step of embodiment, the difference is that, it will be in freeze-drying sea cucumber intestine ovum raw material merging subcritical abstraction device
Extracted using propane, extraction temperature is 35 DEG C, and extraction pressure is 0.4Mpa, after extraction by solvent and sea cucumber intestine ovum crude oil in
Separation pressure 0.2Mpa, separating treatment is carried out at 35 DEG C of separation temperature.
Embodiment 3
It is identical as 1 step of embodiment, the difference is that, it will be in freeze-drying sea cucumber intestine ovum raw material merging subcritical abstraction device
Extracted using butane, extraction temperature is 40 DEG C, and extraction pressure is 0.6Mpa, after extraction by solvent and sea cucumber intestine ovum crude oil in
Separation pressure 0.3Mpa, separating treatment is carried out at 48 DEG C of separation temperature.
Embodiment 4
It is identical as 1 step of embodiment, the difference is that, it will be in freeze-drying sea cucumber intestine ovum raw material merging subcritical abstraction device
It is extracted using iso-butane, extraction temperature is 50 DEG C, and extraction pressure is 0.8Mpa, by solvent and sea cucumber intestine ovum crude oil after extraction
Separating treatment is carried out at separation pressure 0.4Mpa, 55 DEG C of separation temperature.
Embodiment 5
It is identical as 1 step of embodiment, the difference is that, it will be in freeze-drying sea cucumber intestine ovum raw material merging subcritical abstraction device
It is extracted using dimethyl ether, extraction temperature is 55 DEG C, and extraction pressure is 1.0Mpa, by solvent and sea cucumber intestine ovum crude oil after extraction
Separating treatment is carried out at separation pressure 0.5Mpa, 65 DEG C of separation temperature.
Embodiment 6
It is identical as 1 step of embodiment, the difference is that, it is the sodium hydroxide solution of 8 ° of B é in 85 DEG C of height that Baume degrees, which is added,
Alkali-refining deacidification 10s is carried out under the conditions of temperature.
Embodiment 7
It is identical as 1 step of embodiment, the difference is that, it is the sodium hydroxide solution of 10 ° of B é in 90 DEG C that Baume degrees, which is added,
Alkali-refining deacidification 3s is carried out under hot conditions.
Performance test
The properties of product of the embodiment 1-7 sea cucumber intestine egg oil being prepared are analyzed as follows, data are shown in Table 1.
The properties of product data of 1 sea cucumber intestine egg oil of table
It analyzes in conjunction with 1 data of table it is found that the sea cucumber intestine that preparation method provided by through the embodiment of the present invention is prepared
Egg oil its acid value, iodine number, peroxide value reach standard, further, since when extracting using ethane, propane, butane, iso-butane or
Dimethyl ether not only can effectively avoid heat using Freeze Drying Technique processing raw material as Extraction solvent, low boiling point, Yi Huishou
The problems such as handling bring raw material oxidation deterioration can also utmostly keep raw material freshness and nutritional ingredient to retain, extract effect
Rate is up to 95% or more.Compared to traditional extraction process, this method belongs to physical extraction technology, green, low consumption and efficient, operation
Safe and simple, this is particularly important effective extraction of sea cucumber intestine egg oil, can greatly speed up the development and utilization of sea cucumber intestine ovum.
Claims (10)
1. the sub-critical extraction method of sea cucumber intestine egg oil, which comprises the following steps:
It chooses the sea cucumber intestine ovum raw material that pre-sifted is chosen to be freeze-dried, obtains freeze-drying sea cucumber intestine ovum raw material；
Will freeze-drying sea cucumber intestine ovum raw material crush after, be placed in subcritical abstraction device in carry out solvent extraction, after extraction by solvent with
Sea cucumber intestine ovum crude oil carries out separating treatment；
Phosphoric acid is added into sea cucumber intestine ovum crude oil and carries out degumming, sodium hydroxide solution is then added and carries out alkali refining under hot conditions
Depickling after centrifugal treating, obtains alkali refining oil；
Alkali refining oil is dried and is deodorized under vacuum condition, sea cucumber intestine egg oil is obtained.
2. sub-critical extraction method according to claim 1, which is characterized in that the freeze-drying smashed grain of sea cucumber intestine ovum raw material
Diameter >=60 mesh.
3. sub-critical extraction method according to claim 1, which is characterized in that solvent-extracted condition is that extraction temperature is
30-55 DEG C, extraction pressure is 0.2-1.0Mpa.
4. sub-critical extraction method according to claim 1, which is characterized in that solvent extraction solvent for use is ethane, third
At least one of alkane, butane, iso-butane and dimethyl ether.
5. sub-critical extraction method according to claim 1, which is characterized in that divided solvent and sea cucumber intestine ovum crude oil
Condition from processing is that separation pressure is 0.1-0.5Mpa, and separation temperature is 20-65 DEG C.
6. sub-critical extraction method according to claim 1, which is characterized in that the phosphoric acid concentration of addition is 85%, is added
Sodium hydroxide solution Baume degrees be 5-10 ° of B é, hot conditions be 80-90 DEG C, the alkali refining time be 1-15s.
7. the sea cucumber intestine egg oil that the sub-critical extraction method of sea cucumber intestine egg oil described in -6 obtains according to claim 1.
8. sea cucumber intestine egg oil according to claim 7, which is characterized in that the acid value mgKOH/g < of the sea cucumber intestine egg oil
10, iodine number g/100g is 70-150, peroxide value g/100g < 2.
9. cosmetics, which is characterized in that using sea cucumber intestine egg oil according to claim 7 as main component.
10. grease or beverage, which is characterized in that using sea cucumber intestine egg oil according to claim 7 as primary raw material.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808673A (en) * | 2020-07-09 | 2020-10-23 | 江苏海宏制药有限公司 | Subcritical fluid extraction process of sea cucumber oil |
| CN113430047A (en) * | 2021-06-24 | 2021-09-24 | 大连平岛天然产物科技有限公司 | Method for extracting grease and peptide from sea cucumber eggs |
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| CN107699359A (en) * | 2017-11-24 | 2018-02-16 | 黑龙江美丽石岛科技开发有限公司 | A kind of extraction process of sea cucumber intestine egg oil and the method that sea cucumber intestine ovum oil soft capsule is prepared using sea cucumber intestine egg oil |
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