CN108578592A - 预防猪瘟病的制剂及其制备方法 - Google Patents
预防猪瘟病的制剂及其制备方法 Download PDFInfo
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Abstract
本发明公开了一种预防猪瘟病的制剂及其制备方法,利用枯草芽孢杆菌、木醋杆菌和酿酒酵母对南板蓝根茎叶15~20份、决明子5~7份、芦根4~8份、大黄3~5份、仙人掌2~5份、葛根2~5份、大青叶1~5份、知母3~5份、猴面包树叶0.1~0.5份、白头翁1~3份、枇杷叶1~3份和细辛1~3份进行发酵,将中草药活性成分与微生物发酵产物结合来预防猪瘟病。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
Description
技术领域
本发明涉及猪养殖领域,具体是一种预防猪瘟病的制剂。
背景技术
猪瘟病是由猪瘟病毒所引起的传染病,无论猪处于哪一个生长阶段,都有感染猪瘟的可能性,并且猪瘟一年四季易感染和流行。由于其严重的传染性,发病率与病死率很高,所以,猪瘟一旦发生,就会引起猪大量死亡,给养殖户带来严重的经济损失,对养猪业的可持续发展十分不利。特别是近几年来,猪瘟病已经出现了向非典型与温和型发展的变化,这使得猪瘟病的临床诊断难度不断加大。猪瘟的暴发与猪的具体生长环境和个体条件有着密不可分的关系
主要是由环境因素、内在因素和人为因素等引起。
(1)环境因素。猪舍的通风条件较差,保温设施不佳;排粪与排尿的设置不灵活,使得猪舍整体的温度过高;(2)内在因素。第一,猪瘟病毒会有自然感染的情况,这样的感染会引起猪瘟病的发病,还会造成许多猪处于亚临床感染状态。第二,一些猪天生就感染了猪瘟病毒,这主要是因为胎盘感染致使幼仔在出生之时就携带猪瘟病毒。第三,猪本身的健康条件较差,没有足够的免疫力应对病毒的人侵;(3)人为因素。第一,为猪注射的相关防治疫苗的效价较低,免疫程序不合规范,使得许多猪本身不能产生强大的保护力应对病毒的人侵。第二,防疫工作做不到位,不能使所有的猪都进行相关医疗防疫,免疫空档为猪瘟病毒的传播创造了条件。第三,在个别猪出现死亡之后,没有对其死亡原因进行分析,对尸体处理的不得当,致使病毒快速传播,造成大量猪群体性感染。
发明内容
本发明的目的之一在于提供一种可降低CSFV对猪淋巴组织的嗜性,有效防治猪瘟病的制剂。
本发明的目的之二在于提供一种对中草药进行发酵,将中草药活性成分与微生物发酵产物结合来预防猪瘟病的制剂的制备方法。
预防猪瘟病的制剂,成分及其重量份包括:南板蓝根茎叶15~20份、决明子5~7份、芦根4~8份、大黄3~5份、仙人掌2~5份、葛根2~5份、大青叶1~5份、知母3~5份、猴面包树叶0.1~0.5份、白头翁1~3份、枇杷叶1~3份和细辛1~3份。
预防猪瘟病的制剂的制备方法,包括以下步骤:
1)按配方量称取中药,并超微粉碎;
2)在粉碎后的中药中加入6~10倍体积量的水,常温浸泡1~5h,武火煮沸后转换为文火煎煮50~80min,冷却后过滤;在滤渣中加入5~7倍体积的水,武火煮沸后转换为文火煎煮30~60min,冷却过滤,合并两次滤液;
3)在滤液中加入葡萄糖10~15份、牛肉浸膏3~8份和亮氨酰甘氨酸0.1~0.3份,接种5~10份枯草芽孢杆菌、5~10份木醋杆菌和5~10份酿酒酵母,发酵2~5d,发酵温度为30~35℃,发酵pH为5.0~7.5,过滤取滤液,低温浓缩后得到预防猪瘟病的制剂。亮氨酰甘氨酸成分为90~95%L-亮氨酰甘氨酸和5~10%D-亮氨酰甘氨酸。经典猪瘟病毒(Clssical swine fevervirus ,CSFV)吸附在细胞表面,并通过其囊膜糖蛋白Erns和E2细胞膜的融合或经受体介导的胞饮作用进入感染细胞。一方面,本发明预防猪瘟病的制剂中活性成分能降低CSFV对猪淋巴组织的嗜性,阻止CSFV侵入猪的扁桃体及其外周淋巴结的B滤泡和上皮细胞中,明显降低其对猪免疫系统的损伤,因此,当CSFV侵入体内时,猪的免疫系统能快速启动,对病原体进行清除。另一方面,本发明预防猪瘟病的制剂中活性成分能直接对CSFV的脂双层和核衣进行破坏,阻止CSFV 在猪体内快速复制扩增。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
与现有技术相比,本发明的优点在于:利用枯草芽孢杆菌、木醋杆菌和酿酒酵母对中草药进行发酵,将中草药活性成分与微生物发酵产物结合来预防猪瘟病。一方面,本发明预防猪瘟病的制剂中活性成分能降低CSFV对猪淋巴组织的嗜性,阻止CSFV侵入猪的扁桃体及其外周淋巴结的B滤泡和上皮细胞中,明显降低其对猪免疫系统的损伤,因此,当CSFV侵入体内时,猪的免疫系统能快速启动,对病原体进行清除。另一方面,本发明预防猪瘟病的制剂中活性成分能直接对CSFV的脂双层和核衣进行破坏,阻止CSFV 在猪体内快速复制扩增。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
具体实施方式
下面通过实施例对本发明方案作进一步说明:
实施例1:
预防猪瘟病的制剂,成分及其重量份包括:南板蓝根茎叶18份、决明子6份、芦根6份、大黄4份、仙人掌3份、葛根4份、大青叶3份、知母4份、猴面包树叶0.4份、白头翁2份、枇杷叶2份和细辛2份。
预防猪瘟病的制剂的制备方法,包括以下步骤:
1)按配方量称取中药,并超微粉碎;
2)在粉碎后的中药中加入8倍体积量的水,常温浸泡3h,武火煮沸后转换为文火煎煮60min,冷却后过滤;在滤渣中加入6倍体积的水,武火煮沸后转换为文火煎煮40min,冷却过滤,合并两次滤液;
3)在滤液中加入葡萄糖14份、牛肉浸膏6份和亮氨酰甘氨酸0.2份,接种7份枯草芽孢杆菌、8份木醋杆菌和8份酿酒酵母,发酵4d,发酵温度为34℃,发酵pH为6.5,过滤取滤液,低温浓缩后得到预防猪瘟病的制剂。亮氨酰甘氨酸成分为93%L-亮氨酰甘氨酸和7%D-亮氨酰甘氨酸。经典猪瘟病毒(Clssical swine fever virus ,CSFV)吸附在细胞表面,并通过其囊膜糖蛋白Erns和E2细胞膜的融合或经受体介导的胞饮作用进入感染细胞。一方面,本发明预防猪瘟病的制剂中活性成分能降低CSFV对猪淋巴组织的嗜性,阻止CSFV侵入猪的扁桃体及其外周淋巴结的B滤泡和上皮细胞中,明显降低其对猪免疫系统的损伤,因此,当CSFV侵入体内时,猪的免疫系统能快速启动,对病原体进行清除。另一方面,本发明预防猪瘟病的制剂中活性成分能直接对CSFV的脂双层和核衣进行破坏,阻止CSFV 在猪体内快速复制扩增。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
实施例2:
预防猪瘟病的制剂,成分及其重量份包括:南板蓝根茎叶17份、决明子7份、芦根5份、大黄4份、仙人掌4份、葛根4份、大青叶4份、知母4份、猴面包树叶0.4份、白头翁2份、枇杷叶3份和细辛1份。
预防猪瘟病的制剂的制备方法,包括以下步骤:
1)按配方量称取中药,并超微粉碎;
2)在粉碎后的中药中加入8倍体积量的水,常温浸泡4h,武火煮沸后转换为文火煎煮70min,冷却后过滤;在滤渣中加入6倍体积的水,武火煮沸后转换为文火煎煮45min,冷却过滤,合并两次滤液;
3)在滤液中加入葡萄糖12份、牛肉浸膏7份和亮氨酰甘氨酸0.2份,接种7份枯草芽孢杆菌、8份木醋杆菌和7份酿酒酵母,发酵3d,发酵温度为32℃,发酵pH为6.0,过滤取滤液,低温浓缩后得到预防猪瘟病的制剂。亮氨酰甘氨酸成分为94%L-亮氨酰甘氨酸和6%D-亮氨酰甘氨酸。经典猪瘟病毒(Clssical swine fever virus ,CSFV)吸附在细胞表面,并通过其囊膜糖蛋白Erns和E2细胞膜的融合或经受体介导的胞饮作用进入感染细胞。一方面,本发明预防猪瘟病的制剂中活性成分能降低CSFV对猪淋巴组织的嗜性,阻止CSFV侵入猪的扁桃体及其外周淋巴结的B滤泡和上皮细胞中,明显降低其对猪免疫系统的损伤,因此,当CSFV侵入体内时,猪的免疫系统能快速启动,对病原体进行清除。另一方面,本发明预防猪瘟病的制剂中活性成分能直接对CSFV的脂双层和核衣进行破坏,阻止CSFV 在猪体内快速复制扩增。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
实施例3:
预防猪瘟病的制剂,成分及其重量份包括:南板蓝根茎叶17份、决明子6份、芦根5份、大黄4份、仙人掌3份、葛根3份、大青叶3份、知母3份、猴面包树叶0.3份、白头翁1份、枇杷叶1份和细辛3份。
预防猪瘟病的制剂的制备方法,包括以下步骤:
1)按配方量称取中药,并超微粉碎;
2)在粉碎后的中药中加入9倍体积量的水,常温浸泡5h,武火煮沸后转换为文火煎煮70min,冷却后过滤;在滤渣中加入5倍体积的水,武火煮沸后转换为文火煎煮30min,冷却过滤,合并两次滤液;
3)在滤液中加入葡萄糖13份、牛肉浸膏6份和亮氨酰甘氨酸0.2份,接种9份枯草芽孢杆菌、6份木醋杆菌和6份酿酒酵母,发酵4d,发酵温度为33℃,发酵pH为6.0,过滤取滤液,低温浓缩后得到预防猪瘟病的制剂。亮氨酰甘氨酸成分为90~95%L-亮氨酰甘氨酸和5~10%D-亮氨酰甘氨酸。经典猪瘟病毒(Clssical swine fever virus ,CSFV)吸附在细胞表面,并通过其囊膜糖蛋白Erns和E2细胞膜的融合或经受体介导的胞饮作用进入感染细胞。一方面,本发明预防猪瘟病的制剂中活性成分能降低CSFV对猪淋巴组织的嗜性,阻止CSFV侵入猪的扁桃体及其外周淋巴结的B滤泡和上皮细胞中,明显降低其对猪免疫系统的损伤,因此,当CSFV侵入体内时,猪的免疫系统能快速启动,对病原体进行清除。另一方面,本发明预防猪瘟病的制剂中活性成分能直接对CSFV的脂双层和核衣进行破坏,阻止CSFV 在猪体内快速复制扩增。在猪瘟病流行期间,在猪饲料中添加本发明制剂可有效预防猪瘟病的发生,喂养后的猪体质健壮结实,行动灵活轻巧,皮毛光洁,食欲旺盛,体温正常,无拉稀及死亡现象。
本发明的操作步骤中的常规操作为本领域技术人员所熟知,在此不进行赘述。
以上所述的实施例对本发明的技术方案进行了详细说明,应理解的是以上所述仅为本发明的具体实施例,并不用于限制本发明,凡在本发明的原则范围内所做的任何修改、补充或类似方式替代等,均应包含在本发明的保护范围之内。
Claims (4)
1.预防猪瘟病的制剂,其特征在于成分及其重量份包括:南板蓝根茎叶15~20份、决明子5~7份、芦根4~8份、大黄3~5份、仙人掌2~5份、葛根2~5份、大青叶1~5份、知母3~5份、猴面包树叶0.1~0.5份、白头翁1~3份、枇杷叶1~3份和细辛1~3份。
2.预防猪瘟病的制剂的制备方法,其特征在于:包括以下步骤:
1)按配方量称取中药,并超微粉碎;
2)在粉碎后的中药中加入6~10倍体积量的水,常温浸泡1~5h,武火煮沸后转换为文火煎煮50~80min,冷却后过滤;在滤渣中加入5~7倍体积的水,武火煮沸后转换为文火煎煮30~60min,冷却过滤,合并两次滤液;
3)在滤液中加入葡萄糖10~15份、牛肉浸膏3~8份和亮氨酰甘氨酸0.1~0.3份,接种5~10份枯草芽孢杆菌、5~10份木醋杆菌和5~10份酿酒酵母,发酵2~5d,过滤取滤液,低温浓缩后得到预防猪瘟病的制剂。
3.根据权利要求2所述的预防猪瘟病的制剂的制备方法,其特征在于:所述的亮氨酰甘氨酸成分为90~95%L-亮氨酰甘氨酸和5~10%D-亮氨酰甘氨酸。
4.根据权利要求2所述的预防猪瘟病的制剂的制备方法,其特征在于:所述的发酵温度为30~35℃,发酵pH为5.0~7.5。
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