CN108555019A - Restorative procedure of the arbuscular mycorrhiza-Alfalfa homobium to cadmium pollution soil - Google Patents
Restorative procedure of the arbuscular mycorrhiza-Alfalfa homobium to cadmium pollution soil Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a kind of arbuscular mycorrhiza Alfalfa homobiums to the restorative procedure of cadmium pollution soil, including：Step 1: preparing bush mycorrhizal fungi preparation；Step 2: the processing of alfalfa seed；Step 3: the Arbuscular Mycorrhizal Fungi microbial inoculum in the topsoil of cadmium pollution soil；Then alfalfa seed is planted, carries out field management；After growth, harvest plant, dry, shift after centralized burning, reparation of the realization to cadmium pollution soil.Present invention combination microorganism remediation technology and phytoremediation technology, solution microorganism remediation speed is slow, responding time is long, it is high to require soil environment, limit the problem of popularization and application of its large area are as a kind of green recovery technique, it is of low cost using phytoremediation, good comprehensive ecological benefit, the advantages that being especially suitable for being widely applied, microorganism remediation economy does not change the features such as soil intrinsic physicochemical property conveniently, obtained restorative procedure processing capacity is strong, it is economical, conveniently, suit large area to popularize.
Description
Technical field
The present invention relates to a kind of restorative procedures of cadmium pollution soil, and in particular to a kind of arbuscular mycorrhiza-Alfalfa homobium
To the restorative procedure of cadmium pollution soil.
Background technology
Currently, China ploughs by getting worse the problem of the heavy metal pollutions such as cadmium, arsenic, lead, the economic loss thereby resulted in
Also increasing, it was reported that annual China at least 20,000,000,000 yuan of economic loss caused by heavy metal pollution.All heavy metal-polluted
It is the most serious with cadmium pollution in dye.Recently as the industrial expansions such as mining, metallurgy and Cadmium treated, China's agricultural soil
By cadmium pollution also getting worse.Since cadmium cannot be degraded by Soil Microorganism, half-life period is more than 20 years, and pollution is irreversible
Cumulative process；Cadmium is the very strong heavy metal of biogenic migration again, is easily absorbed by plants and accumulates, not only more than certain limit
Yield, the quality of crop are seriously affected, and edible part easily in people's cylinder accumulation and is detrimental to health by food chain.
The toxicity of compound of cadmium is big, and belongs to savings, causes the incubation period of slow poisoning up to 10 to 30 years.In short, in soil
Heavy metal cadmium has drastically influenced the sustainable development of agricultural production.
The complexity of cadmium pollution soil reparation and highly difficult so that there is no a kind of recovery technique of real stability and high efficiency at present
Can meet the needs of reality production；Peripheral doses and chemical remediation can comparatively fast realize the reduction of cadmium content in soil, but it only changes
The existence form of cadmium in soil is become without being thoroughly removed, often there is also expensive, quantities is huge, two secondary rings
The problem of border is polluted；Animal reparation and microorganism remediation have warp as a kind of green recovery technique compared to other repair modes
Ji does not change the characteristics of soil intrinsic physicochemical property conveniently, but it is repaired that speed is slow, responding time is long, to soil environment requirement
High problem limits the popularization and application of its large area.It is not only of low cost using phytoremediation by the environment of cadmium pollution, but also
There is good comprehensive ecological benefit, be especially suitable for being widely applied, but it cannot perform to most the effect of cadmium pollution soil
It is good.
Invention content
It is excellent it is an object of the invention to solve at least the above and/or defect, and provide at least to will be described later
Point.
In order to realize these purposes and other advantages according to the present invention, a kind of arbuscular mycorrhiza-Alfalfa symbiosis is provided
Body includes the following steps the restorative procedure of cadmium pollution soil：
Step 1: arbuscular mycorrhizal fungi is mixed with the numerous matrix of the expansion of sterilizing according to the inoculum concentration that mass ratio is 5~8%
It is even, mixed-matrix is then filled to the 2/3~4/5 of sterile basin；After sowing the Sudan's grass seed, then cover the sterilizing of one layer of 0.5cm
Sandy soil culture medium, hot-house culture；After potting culture 3~4 months, whole cauline leafs of sudangrass above-ground plant parts are cut off, by basin
Drying disposed within 1~2 week harvests all cultures in basin；All cultures are poured on aseptic paper, it will with the tool of disinfection
It after it is minced, is transferred in sterile bag, envelope refrigerates to get bush mycorrhizal fungi preparation；
Step 2: alfalfa seed is placed in cold plasma processor, to clover under the processing power of 150~300W
The unionized width that seed carries out 10~15s penetrates processing, handles using helium as working media, is carried out in vacuum sealing environment；It will
Treated, and alfalfa seed is put into the acetic acid aqueous solution containing 0.5~0.8mg/mL chitosans, at 20~30 DEG C at ultrasound
10min is managed, during supersound process, ozone is passed through into acetic acid aqueous solution, the Ventilation Rate of ozone is 50~100L/
min；The alfalfa seed that obtains that treated；
Step 3: the Arbuscular Mycorrhizal Fungi microbial inoculum in the topsoil of cadmium pollution soil, inoculative proportion is every 500~
20~30g of bush mycorrhizal fungi preparation is added in 800g soil；Then alfalfa seed is planted, planting density is every square metre
5~10 rows, often 60~100 plants of row；Carry out field management；Through growth 60~120 days after, harvest plant, dry, shift after collect
The reparation to cadmium pollution soil is realized in middle burning.
Preferably, all cultures include root system of plant, mycelia, spore, sandy soil culture medium and expand numerous matrix；
It is 1 that the numerous matrix of expansion, which is weight ratio,:2:1:1 river sand, soil, vermiculite, zeolite；The sterilizing for expanding numerous matrix uses high temperature
Sterilizing, moist heat sterilization condition are 121 DEG C of 40~60min of sterilizing, and interval sterilizes primary again after 18~24 hours.
Preferably, in the step 2, further include：Treated alfalfa seed is added in seed soak and impregnates 1
~3h；Naturally dry is then taken out, is then 50~60kV/cm in electric field strength, pulse width is 10~12 μ s, pulse frequency
To handle 80~120s in the impulse electric field of 80~100Hz.
Preferably, the seed soak includes that the raw material of following parts by weight mixes：0.1~0.5 part of gibberellin,
1~3 part of potassium selenite, 0.3~0.5 part of sodium dodecyl aminopropionitrile, 0.5~0.8 part of sodium naphthenate, sodium humate 0.1~
0.3 part, 1~2 part of hydroxyethylethylene diamine tri-acetic acid, 0.5~0.8 part of alkyl-glucoside, 0.3~0.5 part of nano zine oxide, purple
0.5~0.8 part of sweet potato anthocyanin, 1~2 part of sodium alginate, 1~3 part of spirulina extract, 1~3 part of Bulbus Allii Cepae extract, rice washing
150~300 parts of water.
Preferably, in soaking process, it is passed through ozone into seed soak, the Ventilation Rate of ozone is 50~
100L/min。
Preferably, further include before the step 3：Whole cadmium pollution soil is turned over, is then added in the cadmium pollution soil
Soil-repairing agent, turn over it is whole so that renovation agent is mixed well with cadmium pollution soil, the additive amount of the soil-repairing agent is that cadmium is dirty per acre
It contaminates soil and adds 30~50kg.
Preferably, the soil-repairing agent includes the raw material composition of following parts by weight：Attapulgite modified 20~25 parts,
10~15 parts of modified straw biological carbon, 3~5 parts of 2 hydroxybenzoic acid -5- sodium sulfonates, 1~3 part of cycloheptaamylose, humic acid 5~
10 parts, 2~7 parts of quick lime, 3~6 parts of dimercaprol dimercaptopropanol, 4~6 parts of bentonite, 2~5 parts of wood vinegar, 0.5~1 part of polycyclic oxygen amber
Amber acid, 3~5 parts of oyster shell whiting.
Preferably, the preparation method of the soil-repairing agent is：By weight, by 2 hydroxybenzoic acid -5- sodium sulfonates,
Cycloheptaamylose, dimercaprol dimercaptopropanol, wood vinegar, poly-epoxy succinic acid and 100 parts of water are added to the container, and it is molten to stir to get mixing
Liquid；Attapulgite modified, modified straw biological carbon, humic acid, quick lime, bentonite and oyster shell whiting are put into vibrator
It is interior, according to material: ball=1：5~10 ratio is added Ceramic Balls and carries out coarse crushing, obtains coarse powder particle；Take coarse powder particle and mixing
Solution is added in polytetrafluoroethylene (PTFE) ball grinder, and ball milling ball is added and is placed on planetary ball mill, carries out ball milling；Material after ball milling dries
It is dry, obtain soil-repairing agent；The weight ratio of the ball milling ball and coarse powder particle is 5~10：1, the ball mill mixing time is 1~3h,
Rotational speed of ball-mill is 200~300r/min.
Preferably, the attapulgite modified preparation method is：20~30 parts of attapulgite is taken, is added 100 part 0.8
In the hydrochloric acid solution of~1.2mol/L, 30~60min is stirred, is filtered, drying takes 10~25 parts of attapulgite, the molybdic acid of drying
120 parts of 1~3 part of ammonium, 3~5 parts of nitric acid molybdenum, 1~3 part of ammonium oxalate, 1~3 part of iminobisuccinate chelated zinc and water additions
In sealing container, sealing is placed in the electron accelerator of 2.5MeV, 40mA and carries out irradiation stir process, and directly evaporation drying obtains
To attapulgite modified；The radiation dose rate of the irradiation is 200~400kGy/h, and irradiation dose is 400~1000kGy, is stirred
It is 200r/min to mix speed.
Preferably, the preparation method of the modified straw biological carbon is：The acetum for being 6~10% with volume fraction
The pH to 3~4 for adjusting ethanol water, by isobutene triethoxysilane be added in the ethanol water of modulated pH concussion 1~
3h obtains modification liquid；Supercritical reaction apparatus is added in 20~30 parts by weight straw biological charcoals, 50~100 parts by volume modification liquids
In, be passed through carbon dioxide to 40~60MPa after system sealing, be stirred to react 1~3 hour at 40~60 DEG C, pressure release, then plus
The cysteine solution for entering 30~50 parts by volume 0.50mol/L, after system sealing is passed through carbon dioxide to 40~60MPa, and 40
It stirs 1~3 hour, pressure release, filters at~50 DEG C, it is dry, obtain modified straw biological carbon；In the ethanol water ethyl alcohol with
The volume ratio of water is 1:5；The addition of the isobutene triethoxysilane is the 5% of the ethanol water total volume.
The present invention includes at least following advantageous effect：Present invention combination phytoremediation and two kinds of sides of microorganism remediation technology
Method, by the advantages of both to arbuscular mycorrhiza-Alfalfa homobium culture, can perfectly combining, it is economical, conveniently, do not change soil
The intrinsic physicochemical property of earth, and have good comprehensive ecological benefit, it is especially suitable for being widely applied, cadmium pollution soil is repaiied
It is multiple, have the characteristics that good regulation effect, non-secondary pollution, operating cost are low, there is theoretical and application and popularization value well.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific implementation mode：
With reference to embodiment, the present invention is described in further detail, to enable those skilled in the art with reference to specification
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
Arbuscular mycorrhizal fungi of the present invention is Glomus versiforme (Glomus versiforme), derives from Beijing's agriculture
Woods academy of sciences plant nutrient and the Study on Resources institute " Arbuscular Mycorrhizal Fungi In China germplasm resource bank (BGC), number BGC GD01C.
Embodiment 1：
A kind of arbuscular mycorrhiza-Alfalfa homobium includes the following steps the restorative procedure of cadmium pollution soil：
Step 1: be uniformly mixed arbuscular mycorrhizal fungi with the numerous matrix of the expansion of sterilizing according to the inoculum concentration that mass ratio is 5%,
Then mixed-matrix is filled to the 2/3 of sterile basin；After sowing the Sudan's grass seed, then cover the sterilizing sandy soil culture of one layer of 0.5cm
Base, hot-house culture；After potting culture 3 months, whole cauline leafs of sudangrass above-ground plant parts are cut off, by basin drying disposed within
1 week, harvest all cultures in basin；All cultures are poured on aseptic paper, after being minced with the tool of disinfection, are transferred to
In sterile bag, envelope refrigerates to get bush mycorrhizal fungi preparation；All cultures include root system of plant, mycelia, spore
Son, sandy soil culture medium and the numerous matrix of expansion；It is 1 that the numerous matrix of expansion, which is weight ratio,:2:1:1 river sand, soil, vermiculite, zeolite；Institute
The sterilizing for expanding numerous matrix is stated using high-temperature sterilization, moist heat sterilization condition is 121 DEG C of sterilizing 60min, and interval sterilizes again after 24 hours
Once；
Step 2: alfalfa seed is placed in cold plasma processor, to alfalfa seed under the processing power of 200W
The unionized width for carrying out 10s penetrates processing, handles using helium as working media, is carried out in vacuum sealing environment；By treated
Alfalfa seed is put into the acetic acid aqueous solution containing 0.5mg/mL chitosans, and 10min is ultrasonically treated at 20 DEG C, is being ultrasonically treated
During, ozone is passed through into acetic acid aqueous solution, the Ventilation Rate of ozone is 80L/min；The alfalfa seed that obtains that treated；
The mass fraction of acetic acid is 1.2% in the acetic acid aqueous solution；
Step 3: Arbuscular Mycorrhizal Fungi is true in the topsoil of cadmium pollution soil (the full cadmium content of soil is 5.757mg/kg)
Bacteria agent, inoculative proportion are per addition bush mycorrhizal fungi preparation 20g in 500g soil；Then alfalfa seed is planted, is planted
Density is every square metre of 8 rows, often 80 plants of row；Carry out field management；After growth 60~120 days, plant is harvested, dry,
Centralized burning after transfer realizes the reparation to cadmium pollution soil, after testing, after being repaired using the present embodiment restorative procedure,
The full cadmium content of soil is 1.687mg/kg.
Embodiment 2：
A kind of arbuscular mycorrhiza-Alfalfa homobium includes the following steps the restorative procedure of cadmium pollution soil：
Step 1: be uniformly mixed arbuscular mycorrhizal fungi with the numerous matrix of the expansion of sterilizing according to the inoculum concentration that mass ratio is 8%,
Then mixed-matrix is filled to the 4/5 of sterile basin；After sowing the Sudan's grass seed, then cover the sterilizing sandy soil culture of one layer of 0.5cm
Base, hot-house culture；After potting culture 4 months, whole cauline leafs of sudangrass above-ground plant parts are cut off, by basin drying disposed within
2 weeks, harvest all cultures in basin；All cultures are poured on aseptic paper, after being minced with the tool of disinfection, are transferred to
In sterile bag, envelope refrigerates to get bush mycorrhizal fungi preparation；All cultures include root system of plant, mycelia, spore
Son, sandy soil culture medium and the numerous matrix of expansion, it is 1 that the numerous matrix of expansion, which is weight ratio,:2:1:1 river sand, soil, vermiculite, zeolite；Institute
The sterilizing for expanding numerous matrix is stated using high-temperature sterilization, moist heat sterilization condition is 121 DEG C of sterilizing 60min, and interval sterilizes again after 18 hours
Once；
Step 2: alfalfa seed is placed in cold plasma processor, to alfalfa seed under the processing power of 250W
The unionized width for carrying out 15s penetrates processing, handles using helium as working media, is carried out in vacuum sealing environment；By treated
Alfalfa seed is put into the acetic acid aqueous solution containing 0.8mg/mL chitosans, and 10min is ultrasonically treated at 30 DEG C, is being ultrasonically treated
During, ozone is passed through into acetic acid aqueous solution, the Ventilation Rate of ozone is 100L/min；The clover kind that obtains that treated
Son；The mass fraction of acetic acid is 1.2% in the acetic acid aqueous solution；
Step 3: Arbuscular Mycorrhizal Fungi is true in the topsoil of cadmium pollution soil (the full cadmium content of soil is 5.682mg/kg)
Bacteria agent, inoculative proportion are per addition bush mycorrhizal fungi preparation 30g in 800g soil；Then alfalfa seed is planted, is planted
Density is every square metre of 10 rows, often 100 plants of row；Carry out field management；After growth 60~120 days, plant is harvested, is shone
Centralized burning after dry, transfer, realizes the reparation to cadmium pollution soil.After testing, it is repaired using the present embodiment restorative procedure
Afterwards, the full cadmium content of soil is 1.567mg/kg.
Embodiment 3：
In the step 2, further include：Treated alfalfa seed is added in seed soak and impregnates 2h；It then takes out
Then naturally dry is 60kV/cm in electric field strength, pulse width is 12 μ s, and pulse frequency is in the impulse electric field of 100Hz
Manage 80s；In soaking process, ozone is passed through into seed soak, the Ventilation Rate of ozone is 100L/min；
The seed soak includes that the raw material of following parts by weight mixes：0.3 part of gibberellin, 2 parts of potassium selenite, ten
0.5 part of dialkyl amido sodium propionate, 0.5 part of sodium naphthenate, 0.3 part of sodium humate, 2 parts of hydroxyethylethylene diamine tri-acetic acid, alkyl
0.8 part of glucoside, 0.5 part of nano zine oxide, 0.8 part of purple sweet potato cyanidin, 1 part of sodium alginate, 1 part of spirulina extract,
1 part of Bulbus Allii Cepae extract, 300 parts of rice washing water.
Remaining technological parameter and method with it is identical in embodiment 1.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 1.158mg/kg.
Embodiment 4：
In the step 2, further include：Treated alfalfa seed is added in seed soak and impregnates 3h；It then takes out
Then naturally dry is 50kV/cm in electric field strength, pulse width is 10 μ s, and pulse frequency is in the impulse electric field of 80Hz
Manage 120s；In soaking process, ozone is passed through into seed soak, the Ventilation Rate of ozone is 80L/min.
The seed soak includes that the raw material of following parts by weight mixes：0.5 part of gibberellin, 3 parts of potassium selenite, ten
0.3 part of dialkyl amido sodium propionate, 0.8 part of sodium naphthenate, 0.2 part of sodium humate, 2 parts of hydroxyethylethylene diamine tri-acetic acid, alkyl
0.6 part of glucoside, 0.5 part of nano zine oxide, 0.7 part of purple sweet potato cyanidin, 2 parts of sodium alginate, 1 part of spirulina extract,
2 parts of Bulbus Allii Cepae extract, 300 parts of rice washing water.
Remaining technological parameter and method with it is identical in embodiment 1.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 1.16mg/kg.
Embodiment 5：
Further include before the step 3：Whole cadmium pollution soil is turned over, then adds soil remediation in the cadmium pollution soil
Agent, turning over whole makes renovation agent be mixed well with cadmium pollution soil；The additive amount of the soil-repairing agent is that cadmium pollution soil adds per acre
Add 30kg；
The soil-repairing agent includes the raw material composition of following parts by weight：Attapulgite modified 25 parts, modified straw biology
10 parts of 3 parts of 2 hydroxybenzoic acid -5- sodium sulfonates of carbon, 2 parts of cycloheptaamylose, 8 parts of humic acid, 5 parts of quick lime, dimercaprol dimercaptopropanol 5
Part, 5 parts of bentonite, 3 parts of wood vinegar, 3~5 parts of 0.5 part of poly-epoxy succinic acid, oyster shell whiting；
The preparation method of the soil-repairing agent is：By weight, 2 hydroxybenzoic acid -5- sodium sulfonates, β-ring-type are pasted
Essence, dimercaprol dimercaptopropanol, wood vinegar, poly-epoxy succinic acid and 100 parts of water are added to the container, and stir to get mixed solution；It will be modified
Attapulgite, modified straw biological carbon, humic acid, quick lime, bentonite and oyster shell whiting are put into vibrator, according to material:
Ball=1：5 ratio is added Ceramic Balls and carries out coarse crushing, obtains coarse powder particle；Take coarse powder particle and mixed solution that polytetrafluoro is added
In ethylene ball grinder, ball milling ball is added and is placed on planetary ball mill, carries out ball milling；Drying materials after ball milling obtain soil and repair
Multiple agent；The weight ratio of the ball milling ball and coarse powder particle is 5：1, the ball mill mixing time is 1h, rotational speed of ball-mill 300r/min.
Remaining technological parameter and method with it is identical in embodiment 1.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.85mg/kg.
Embodiment 6：
Further include before the step 3：Whole cadmium pollution soil is turned over, then adds soil remediation in the cadmium pollution soil
Agent, turn over it is whole so that renovation agent is mixed well with cadmium pollution soil, the additive amount of the soil-repairing agent is that cadmium pollution soil adds per acre
Add 50kg；
The soil-repairing agent includes the raw material composition of following parts by weight：Attapulgite modified 20 parts, modified straw biology
15 parts of 3 parts of 2 hydroxybenzoic acid -5- sodium sulfonates of carbon, 2 parts of cycloheptaamylose, 8 parts of humic acid, 5 parts of quick lime, dimercaprol dimercaptopropanol 5
Part, 5 parts of bentonite, 3 parts of wood vinegar, 3 parts of 0.5 part of poly-epoxy succinic acid, oyster shell whiting；
The preparation method of the soil-repairing agent is：By weight, 2 hydroxybenzoic acid -5- sodium sulfonates, β-ring-type are pasted
Essence, dimercaprol dimercaptopropanol, wood vinegar, poly-epoxy succinic acid and 100 parts of water are added to the container, and stir to get mixed solution；It will be modified
Attapulgite, modified straw biological carbon, humic acid, quick lime, bentonite and oyster shell whiting are put into vibrator, according to material:
Ball=1：8 ratio is added Ceramic Balls and carries out coarse crushing, obtains coarse powder particle；Take coarse powder particle and mixed solution that polytetrafluoro is added
In ethylene ball grinder, ball milling ball is added and is placed on planetary ball mill, carries out ball milling；Drying materials after ball milling obtain soil and repair
Multiple agent；The weight ratio of the ball milling ball and coarse powder particle is 10：1, the ball mill mixing time is 2h, rotational speed of ball-mill 200r/min.
Remaining technological parameter and method with it is identical in embodiment 1.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.83mg/kg.
Embodiment 7：
The attapulgite modified preparation method is：30 parts of attapulgite is taken, the hydrochloric acid that 100 parts of 1.2mol/L are added is molten
In liquid, stir 60min, filter, drying, take drying 3 parts of 5 parts of 1 part of 25 parts of attapulgite, ammonium molybdate, nitric acid molybdenum, ammonium oxalate,
120 parts of 1 part of iminobisuccinate chelated zinc and water are added in sealing container, and sealing, the electronics for being placed in 2.5MeV, 40mA adds
Irradiation stir process is carried out in fast device, directly evaporation drying obtains attapulgite modified；The radiation dose rate of the irradiation is
200kGy/h, irradiation dose 400kGy, mixing speed 200r/min.
Remaining technological parameter and method with it is identical in embodiment 5.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.28mg/kg.
Embodiment 8：
The attapulgite modified preparation method is：25 parts of attapulgite is taken, the hydrochloric acid solution of 100 parts of 1mol/L is added
In, 60min is stirred, is filtered, drying takes 2 parts of 3 parts of 2 parts of 20 parts of attapulgite, ammonium molybdate, nitric acid molybdenum, ammonium oxalate, the Asia of drying
120 parts of 2 parts of amino disuccinate chelated zinc and water are added in sealing container, and sealing, the electronics for being placed in 2.5MeV, 40mA accelerates
Irradiation stir process is carried out in device, directly evaporation drying obtains attapulgite modified；The radiation dose rate of the irradiation is
300kGy/h, irradiation dose 600kGy, mixing speed 200r/min.
Remaining technological parameter and method with it is identical in embodiment 5.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.3mg/kg.
Embodiment 9：
The preparation method of the modified straw biological carbon is：It is water-soluble that the acetum for being 10% with volume fraction adjusts ethyl alcohol
The pH to 4 of liquid, isobutene triethoxysilane is added in the ethanol water of modulated pH and shakes 1h, obtains modification liquid；By 20
Parts by weight straw biological charcoal, 50 parts by volume modification liquids are added in supercritical reaction apparatus, and carbon dioxide is passed through after system sealing
It to 40MPa, is stirred to react at 40 DEG C 3 hours, pressure release, the cysteine solution of 50 parts by volume 0.50mol/L is then added, in body
Carbon dioxide is passed through to 60MPa after system's sealing, stir 1 hour at 50 DEG C, pressure release, is filtered, it is dry, it is biological to obtain modified straw
Carbon；The volume ratio of ethyl alcohol and water is 1 in the ethanol water:5；The addition of the isobutene triethoxysilane is described
The 5% of ethanol water total volume, by the modification to straw biological carbon, it is in porous honeycomb to make it, and specific surface area increases.
Remaining technological parameter and method with it is identical in embodiment 5.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.52mg/kg.
Embodiment 10：
The preparation method of the modified straw biological carbon is：It is water-soluble that the acetum for being 10% with volume fraction adjusts ethyl alcohol
The pH to 3 of liquid, isobutene triethoxysilane is added in the ethanol water of modulated pH and shakes 1h, obtains modification liquid；By 30
Parts by weight straw biological charcoal, 100 parts by volume modification liquids are added in supercritical reaction apparatus, and carbon dioxide is passed through after system sealing
It to 40MPa, is stirred to react at 60 DEG C 2 hours, pressure release, the cysteine solution of 50 parts by volume 0.50mol/L is then added, in body
Carbon dioxide is passed through to 40MPa after system's sealing, stir 2 hours at 50 DEG C, pressure release, is filtered, it is dry, it is biological to obtain modified straw
Carbon；The volume ratio of ethyl alcohol and water is 1 in the ethanol water:5；The addition of the isobutene triethoxysilane is described
The 5% of ethanol water total volume.
Remaining technological parameter and method with it is identical in embodiment 5.After testing, using the present embodiment restorative procedure into
After row is repaired, the full cadmium content of soil is 0.55mg/kg.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and example shown and described herein.
Claims (10)
1. a kind of arbuscular mycorrhiza-Alfalfa homobium is to the restorative procedure of cadmium pollution soil, which is characterized in that including following step
Suddenly：
Step 1: be uniformly mixed arbuscular mycorrhizal fungi with the numerous matrix of the expansion of sterilizing according to the inoculum concentration that mass ratio is 5~8%,
Then mixed-matrix is filled to the 2/3~4/5 of sterile basin；After sowing the Sudan's grass seed, then cover the sterilizing sandy soil of one layer of 0.5cm
Culture medium, hot-house culture；After potting culture 3~4 months, whole cauline leafs of sudangrass above-ground plant parts are cut off, basin is placed in
Indoor seasoning 1~2 week harvests all cultures in basin；All cultures are poured on aseptic paper, are chopped with the tool of disinfection
It after broken, is transferred in sterile bag, envelope refrigerates to get bush mycorrhizal fungi preparation；
Step 2: alfalfa seed is placed in cold plasma processor, to alfalfa seed under the processing power of 150~300W
The unionized width for carrying out 10~15s penetrates processing, handles using helium as working media, is carried out in vacuum sealing environment；It will processing
Alfalfa seed afterwards is put into the acetic acid aqueous solution containing 0.5~0.8mg/mL chitosans, is ultrasonically treated at 20~30 DEG C
10min is passed through ozone into acetic acid aqueous solution, the Ventilation Rate of ozone is 50~100L/min during supersound process；
The alfalfa seed that obtains that treated；
Step 3: the Arbuscular Mycorrhizal Fungi microbial inoculum in the topsoil of cadmium pollution soil, inoculative proportion is every 500~800g soil
20~30g of bush mycorrhizal fungi preparation is added in earth；Then alfalfa seed is planted, planting density is every square metre 5~10
It goes, often 60~100 plants of row；Carry out field management；After growth 60~120 days, harvest plant, dry, shift after concentrate and burn
It burns, realizes the reparation to cadmium pollution soil.
2. arbuscular mycorrhiza as described in claim 1-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In all cultures include root system of plant, mycelia, spore, sandy soil culture medium and expand numerous matrix；It is described to expand numerous matrix and be
Weight ratio is 1:2:1:1 river sand, soil, vermiculite, zeolite；The sterilizing for expanding numerous matrix uses high-temperature sterilization, moist heat sterilization
Condition is 121 DEG C of 40~60min of sterilizing, and interval sterilizes primary again after 18~24 hours.
3. arbuscular mycorrhiza as described in claim 1-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In in the step 2, further including：By treated, 1~3h of immersion in seed soak is added in alfalfa seed；It then takes out certainly
It so dries, is then 50~60kV/cm in electric field strength, pulse width is 10~12 μ s, and pulse frequency is the arteries and veins of 80~100Hz
Rush 80~120s of processing in electric field.
4. arbuscular mycorrhiza as claimed in claim 3-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the seed soak includes that the raw material of following parts by weight mixes：0.1~0.5 part of gibberellin, potassium selenite 1~3
Part, 0.3~0.5 part of sodium dodecyl aminopropionitrile, 0.5~0.8 part of sodium naphthenate, 0.1~0.3 part of sodium humate, ethoxy
1~2 part of ethylenediamine triacetic acid, 0.5~0.8 part of alkyl-glucoside, 0.3~0.5 part of nano zine oxide, purple sweet potato cyanidin
0.5~0.8 part, 1~2 part of sodium alginate, 1~3 part of spirulina extract, 1~3 part of Bulbus Allii Cepae extract, rice washing water 150~300
Part.
5. arbuscular mycorrhiza as claimed in claim 3-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In in soaking process, being passed through ozone into seed soak, the Ventilation Rate of ozone is 50~100L/min.
6. arbuscular mycorrhiza as described in claim 1-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the step 3 further includes before：Whole cadmium pollution soil is turned over, then soil-repairing agent is added in the cadmium pollution soil, turns over
It is whole that renovation agent is made to be mixed well with cadmium pollution soil, the additive amount of the soil-repairing agent be cadmium pollution soil addition 30 per acre~
50kg。
7. arbuscular mycorrhiza as claimed in claim 6-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the soil-repairing agent includes the raw material composition of following parts by weight：Attapulgite modified 20~25 parts, modified straw biological carbon
10~15 parts, 3~5 parts of 2 hydroxybenzoic acid -5- sodium sulfonates, 1~3 part of cycloheptaamylose, 5~10 parts of humic acid, quick lime 2~
7 parts, 3~6 parts of dimercaprol dimercaptopropanol, 4~6 parts of bentonite, 2~5 parts of wood vinegar, 0.5~1 part of poly-epoxy succinic acid, oyster shell whiting 3~
5 parts.
8. arbuscular mycorrhiza as claimed in claim 7-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the preparation method of the soil-repairing agent is：By weight, by 2 hydroxybenzoic acid -5- sodium sulfonates, cycloheptaamylose, two
Mercaprol, wood vinegar, poly-epoxy succinic acid and 100 parts of water are added to the container, and stir to get mixed solution；By modified attapulgite
Soil, modified straw biological carbon, humic acid, quick lime, bentonite and oyster shell whiting are put into vibrator, according to material: ball=1：5
~10 ratio is added Ceramic Balls and carries out coarse crushing, obtains coarse powder particle；Take coarse powder particle and mixed solution that polytetrafluoroethyl-ne is added
In alkene ball grinder, ball milling ball is added and is placed on planetary ball mill, carries out ball milling；Drying materials after ball milling, obtain soil remediation
Agent；The weight ratio of the ball milling ball and coarse powder particle is 5~10：1, the ball mill mixing time be 1~3h, rotational speed of ball-mill be 200~
300r/min。
9. arbuscular mycorrhiza as claimed in claim 7-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the attapulgite modified preparation method is：20~30 parts of attapulgite is taken, the salt of 100 parts of 0.8~1.2mol/L is added
In acid solution, 30~60min is stirred, is filtered, drying takes 1~3 part of 10~25 parts of attapulgite, ammonium molybdate, the nitric acid molybdenum of drying
120 parts of 3~5 parts, 1~3 part of ammonium oxalate, 1~3 part of iminobisuccinate chelated zinc and water are added in sealing container, sealing,
It is placed in the electron accelerator of 2.5MeV, 40mA and carries out irradiation stir process, directly evaporation drying obtains attapulgite modified；
The radiation dose rate of the irradiation is 200~400kGy/h, and irradiation dose is 400~1000kGy, mixing speed 200r/
min。
10. arbuscular mycorrhiza as claimed in claim 7-Alfalfa homobium exists to the restorative procedure of cadmium pollution soil, feature
In the preparation method of the modified straw biological carbon is：The acetum for being 6~10% with volume fraction adjusts ethanol water
PH to 3~4,1~3h of concussion is added in the ethanol water of modulated pH in isobutene triethoxysilane, obtains modification liquid；
20~30 parts by weight straw biological charcoals, 50~100 parts by volume modification liquids are added in supercritical reaction apparatus, after system sealing
Carbon dioxide is passed through to 40~60MPa, is stirred to react 1~3 hour at 40~60 DEG C, pressure release, 30~50 parts by volume are then added
The cysteine solution of 0.50mol/L, after system sealing is passed through carbon dioxide to 40~60MPa, and stirring 1 at 40~50 DEG C~
3 hours, pressure release was filtered, dry, obtains modified straw biological carbon；The volume ratio of ethyl alcohol and water is 1 in the ethanol water:
5；The addition of the isobutene triethoxysilane is the 5% of the ethanol water total volume.
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