CN108552226A - A kind of preparation method of high-performance bio insecticide - Google Patents
A kind of preparation method of high-performance bio insecticide Download PDFInfo
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- CN108552226A CN108552226A CN201810568157.2A CN201810568157A CN108552226A CN 108552226 A CN108552226 A CN 108552226A CN 201810568157 A CN201810568157 A CN 201810568157A CN 108552226 A CN108552226 A CN 108552226A
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- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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Abstract
The invention discloses a kind of preparation methods of high-performance bio insecticide, belong to preparation technique of pesticide field.The present invention first pre-processes deionized water through magnetization; bacillus thuringiensis, Aschersonia can be stimulated, do not strangle bacterium production spore performance; and then wherein by food and digestive ferment package; it is digested and assimilated into enteron aisle, to destroy and penetrate goldbeater's skin, prevents insect normally husking and metamorphosis; carry out desinsection; by using methyl polyethylene glycol, succinic anhydride as raw material, forming the protection to the activated centre of producing enzyme, deinsectization performance is improved；The infiltration that L canavanine, cinnamic acid, P coumaric acids promote deinsectization active constituent is added; it is added simultaneously and lures pest nutriment; grazing rate of the pest to insecticide is improved, the Congo red absorption by ultraviolet light is added, protection, contact efficacy enhancing is formed to active microbial inoculum.The present invention solve the problems, such as current insecticide deinsectization inefficient, can develop immunity to drugs to pest, deinsectization effective time it is short.
Description
Technical field
The invention belongs to preparation technique of pesticide fields, and in particular to a kind of preparation method of high-performance bio insecticide.
Background technology
With the popularization of urban landscaping, more and more mosquitoes can be hidden in afforestation thick grass, can give passerby or
Person gardens leisure person brings harm, and the existing method for eliminating mosquito is mostly sprinkling medicament, with the extension of time, medicament
Constantly volatilization, drug effect decrease, can only play the effect of of short duration mosquito-proof deinsectization, especially meet rainy weather, the medicine of sprinkling
Agent is directly thrown into the eastward flowing stream, and cannot play the effect of due mosquito-proof deinsectization, and then causes the afforestation to promote the well-being of mankind at people
Health threat where.Not only control effect is poor for current control of insect, but also is directed to different pests, also to use different
Insecticide expends a large amount of human and material resources and financial resources cost, can also cause the pollution of environment, and single-activity is used continuously
The insecticide of ingredient easy tos produce drug resistance.In the today for advocating green and environmental protection, a variety of high poisons and high residue chemicals
It is limited the quantity or is stopped production.The pesticides such as organophosphor, pyrethroid, benzoyl area kind and biochemistry have made original poison through compounded combination
Property decrease to a certain extent, but due to using year after year as a result, polypide drug resistance and drug resistance is caused to reinforce year by year, hand over
It is also not very ideal for the effect used.The practical amount of dilution of Agricultural deinsectization agent limits the use of range, agricultural product far beyond it
It learns object residual and non-harmful requirement is far not achieved, increased pesticide demand causes the aggravation of rural environmental pollution year by year, residual
Object is stayed more to can not be ignored the harm of human body.
Invention content
The technical problems to be solved by the invention：Develop immunity to drugs to pest for current insecticide deinsectization inefficient, meeting,
Deinsectization effective time short problem, provides a kind of preparation method of high-performance bio insecticide.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of biological high-efficiency insecticide, which is characterized in that the preparation method includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 1 ~ 2h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain
Pretreatment deionized water takes 5 ~ 8 portions of beef extracts, 20 ~ 30 parts of mushroom brans, 2 ~ 4 parts of soybean cake powders, 10 ~ 13 portions of eggs according to the mass fraction
White peptone, 3 ~ 5 parts of glucose, 2 ~ 4 parts of sodium chloride, 0.3 ~ 0.5 part of ferric sulfate, 0.3 ~ 0.6 part of magnesium sulfate, 0.3 ~ 0.5 part of di(2-ethylhexyl)phosphate
Hydrogen potassium, 0.03 ~ 0.06 part of manganese sulfate, 1000 parts of pretreatment deionized water mixing, sterilizing obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 3 ~ 5：1~3：1 ~ 3 mixing, obtains mix bacterium agent, takes
Mix bacterium agent is seeded to by 4 ~ 6% inoculum concentration in sterilizing object, and in 25 ~ 30 DEG C of fermented and cultureds, centrifugation takes precipitation drying, obtains drying
Object, it is spare；
（3）Take methyl polyethylene glycol in mass ratio 4 ~ 7：1 ~ 3 is added succinic anhydride, adds methyl polyethylene glycol quality 10 ~ 20%
N,N-Dimethylformamide, be stirred, be cooled to room temperature in 90 ~ 95 DEG C, obtain cooling thing, take cooling thing in mass ratio 8 ~
10：1 ~ 4 is added ether, and in 1 ~ 4 DEG C of standing, filtering takes filter residue in mass ratio 1 ~ 3：10~15：14 ~ 18 are added N- hydroxysuccinimidyl acyls
Imines, dicyclohexylcarbodiimide are stirred in 25 ~ 30 DEG C, add the N of filter residue quality 20 ~ 30%, N- dimethyl formyls
Amine is stirred, and filtering takes filtrate in mass ratio 1：3 ~ 5 are added ether, take precipitation a；
（4）Take step（2）Spare dried object in mass ratio 1：10 ~ 15 are added borate buffer mixing, add dry matter
4 ~ 7 times of precipitation a of amount keeps the temperature 1 ~ 2h in 30 ~ 38 DEG C, obtains mixed liquor, according to the mass fraction, take 30 ~ 50 portions of mixed liquors, 3 ~ 6 parts
Sour additive, 10 ~ 15 parts of feeding stimulants, 0.4 ~ 0.7 part Congo red, 8 ~ 12 parts of sodium alginates, 30 ~ 40 parts of water, in 30 ~ 40 DEG C
It is stirred, obtains high-performance bio insecticide.
The step（4）Middle acid additive is to take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 1 ~ 3：2~5：1
Mixing is to get sour additive.
The step（4）Middle feeding stimulant be according to the mass fraction, take 3 ~ 6 parts of honey, 0.3 ~ 0.8 part of l-Alanine,
0.4 ~ 0.7 part of r- aminobutyric acid, 2 ~ 5 portions of corn oils, 10 ~ 15 portions of corn flour, 2 ~ 5 parts of cotton leafs, 6 ~ 9 parts of cottonseed mixing, must take
Eat stimulant.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention first pre-processes deionized water through magnetization, can stimulate bacillus thuringiensis, Aschersonia, not strangle bacterium
Spore performance is produced, bacterial activity is stimulated, thus generates terpene, flavonoids, anthraquinones, due to II type in stimulation insect enteric cavity
Funnel is generated by the cell of glandular stomach, it can synthesize chitin when food passes through glandular stomach, so by food and
Digestive ferment wraps up wherein, is digested and assimilated into enteron aisle, and bacillus thuringiensis, Aschersonia, Le bacterium can secrete chitinase,
Funnel is hydrolyzed, to destruction and penetrates goldbeater's skin, into the lymphocyte of host, prevents insect normally husking and abnormal, progress
Desinsection, by as adding mushroom bran in fermentation medium, being rich in the chitin of colloidal type, chitinase can be promoted to secrete, improve
Deinsectization activity, while these types of mushroom is entered by mode of infection by pest body wall, production spore ability improves, and improves and infects effect
Rate, survival period length, the tolerance of spore are strong, being capable of long-acting deinsectization into that will not develop immunity to drugs in vivo；
（2）Several deinsectization active microbial inoculums that the present invention is added, shadow can be caused under the external conditions such as ultraviolet irradiation to microbial activity
It rings, producing enzyme is easy inactivation, by using methyl polyethylene glycol, succinic anhydride as raw material, being activated to production through n-hydroxysuccinimide
The amino on enzyme surface is modified, the alpha-amido or lysine side-chain of the polyethylene glycol after activation and protein surface N-terminal
δ-amino covalence be combined into stable phthalein amine key, form the protection to the activated centre of producing enzyme, ultraviolet light, heat, acid,
The deinsectization performance of high activity can be still kept under the conditions of alkali；
（3）The present invention be added L- canavanine, cinnamic acid, P- coumaric acids change in pest larva the film character of intestines and Malpighian tube and
Its permeability and active transport mode promote the infiltration of deinsectization active constituent, while being added and luring pest nutriment, improve
Pest is added the Congo red absorption by ultraviolet light and forms protection, contact efficacy to active microbial inoculum to the grazing rate of insecticide
Enhancing.
Specific implementation mode
Bacillus thuringiensis, does not strangle bacterium at Aschersonia：In -1 ~ 4 DEG C of laboratory slant preservations.
Mushroom bran：The cultivation fertilizer after needle mushroom is cultivated, wheat bran, cotton seed hulls, sawdust, quick lime are mainly contained, white lime crushes
40 mesh sieve is crossed, sieving particle is collected, it is dry to get mushroom bran.
Sour additive：Take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 1 ~ 3：2~5：1 mixing is added to get acid
Object.
Feeding stimulant：According to the mass fraction, 3 ~ 6 parts of honey, 0.3 ~ 0.8 part of l-Alanine, 0.4 ~ 0.7 part of r- amino are taken
Butyric acid, 2 ~ 5 portions of corn oils, 10 ~ 15 portions of corn flour, 2 ~ 5 parts of cotton leafs, 6 ~ 9 parts of cottonseed mixing, obtain feeding stimulant.
A kind of preparation method of high-performance bio insecticide, includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 1 ~ 2h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain
Pretreatment deionized water takes 5 ~ 8 portions of beef extracts, 20 ~ 30 parts of mushroom brans, 2 ~ 4 parts of soybean cake powders, 10 ~ 13 portions of eggs according to the mass fraction
White peptone, 3 ~ 5 parts of glucose, 2 ~ 4 parts of sodium chloride, 0.3 ~ 0.5 part of ferric sulfate, 0.3 ~ 0.6 part of magnesium sulfate, 0.3 ~ 0.5 part of di(2-ethylhexyl)phosphate
Hydrogen potassium, 0.03 ~ 0.06 part of manganese sulfate, 1000 parts of pretreatment deionized waters, pH7.5,121 DEG C of sterilizing 20min obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 3 ~ 5：1~3：1 ~ 3 mixing, obtains mix bacterium agent, takes
Mix bacterium agent is seeded to by 4 ~ 6% inoculum concentration in sterilizing object, and in 25 ~ 30 DEG C of 24 ~ 36h of fermented and cultured, centrifugation takes precipitation drying,
Dried object is obtained, it is spare；
（3）Take methyl polyethylene glycol in mass ratio 4 ~ 7：1 ~ 3 is added succinic anhydride, adds methyl polyethylene glycol quality 10 ~ 20%
N,N-Dimethylformamide, be stirred 2 ~ 3h in 90 ~ 95 DEG C, be cooled to room temperature, obtain cooling thing, take cooling thing in mass ratio
8~10：1 ~ 4 is added ether, and 30 ~ 40min is stood in 1 ~ 4 DEG C, and filtering takes filter residue in mass ratio 1 ~ 3：10~15：14 ~ 18 are added N-
HOSu NHS, dicyclohexylcarbodiimide are stirred 30 ~ 40min in 25 ~ 30 DEG C, add filter residue quality 20 ~
30% n,N-Dimethylformamide, it is stirred 18 ~ for 24 hours, filtering takes filtrate in mass ratio 1：3 ~ 5 are added ether, take precipitation
a；
（4）Take step（2）Spare dried object in mass ratio 1：10 ~ 15 borate buffers that a concentration of 0.1mol/L is added mix
It closes, adds the precipitation a of 4 ~ 7 times of dried object quality, keep the temperature 1 ~ 2h in 30 ~ 38 DEG C, obtain mixed liquor, according to the mass fraction, take 30 ~
50 portions of mixed liquors, 3 ~ 6 parts of sour additives, 10 ~ 15 parts of feeding stimulants, 0.4 ~ 0.7 part Congo red, 8 ~ 12 parts of sodium alginates, 30 ~
40 parts of water are stirred 1 ~ 2h in 30 ~ 40 DEG C, obtain high-performance bio insecticide.
Bacillus thuringiensis, does not strangle bacterium at Aschersonia：In -1 DEG C of laboratory slant preservation.
Mushroom bran：The cultivation fertilizer after needle mushroom is cultivated, wheat bran, cotton seed hulls, sawdust, quick lime are mainly contained, white lime crushes
40 mesh sieve is crossed, sieving particle is collected, it is dry to get mushroom bran.
Sour additive：Take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 1：2：1 mixing is to get sour additive.
Feeding stimulant：According to the mass fraction, 3 parts of honey, 0.3 part of l-Alanine, 0.4 part of r- aminobutyric acid, 2 parts of jade are taken
Rice bran oil, 10 portions of corn flour, 2 parts of cotton leafs, 6 parts of cottonseed mixing, obtain feeding stimulant.
A kind of preparation method of high-performance bio insecticide, includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 1h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain pre-
Processing deionized water takes 5 portions of beef extracts, 20 parts of mushroom brans, 2 parts of soybean cake powders, 10 parts of peptones, 3 portions of grapes according to the mass fraction
Sugar, 2 parts of sodium chloride, 0.3 part of ferric sulfate, 0.3 part of magnesium sulfate, 0.3 part of potassium dihydrogen phosphate, 0.03 part of manganese sulfate, 1000 parts of pre- places
Deionized water, pH7.5 are managed, 121 DEG C of sterilizing 20min obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 3：1：1 mixing, obtains mix bacterium agent, takes Mixed Microbes
Agent is seeded to by 4% inoculum concentration in sterilizing object, and for 24 hours in 25 DEG C of fermented and cultureds, centrifugation takes precipitation drying, obtains dried object, spare；
（3）Take methyl polyethylene glycol in mass ratio 4：1 is added succinic anhydride, adds the N, N- of methyl polyethylene glycol quality 10%
Dimethylformamide is stirred 2h in 90 DEG C, is cooled to room temperature, obtains cooling thing, takes cooling thing in mass ratio 8：1 is added second
Ether, 30min is stood in 1 DEG C, and filtering takes filter residue in mass ratio 1：10：14 are added n-hydroxysuccinimide, dicyclohexyl carbon two
Imines is stirred 30min in 25 DEG C, adds the n,N-Dimethylformamide of filter residue quality 20%, is stirred 18h, mistake
Filter, takes filtrate in mass ratio 1：3 are added ether, take precipitation a；
（4）Take step（2）Spare dried object in mass ratio 1：10 are added the borate buffer mixing of a concentration of 0.1mol/L, then
The precipitation a of 4 times of dried object quality is added, keeps the temperature 1h in 30 ~ 38 DEG C, obtains mixed liquor, according to the mass fraction, take 30 parts of mixed liquors, 3
Part sour additive, 10 parts of feeding stimulants, 0.4 part Congo red, 8 parts of sodium alginates, 30 parts of water, is stirred 1h in 30 DEG C, obtains
High-performance bio insecticide.
Bacillus thuringiensis, does not strangle bacterium at Aschersonia：In 2 DEG C of laboratory slant preservations.
Mushroom bran：The cultivation fertilizer after needle mushroom is cultivated, wheat bran, cotton seed hulls, sawdust, quick lime are mainly contained, white lime crushes
40 mesh sieve is crossed, sieving particle is collected, it is dry to get mushroom bran.
Sour additive：Take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 2：4：1 mixing is to get sour additive.
Feeding stimulant：According to the mass fraction, 5 parts of honey, 0.5 part of l-Alanine, 0.6 part of r- aminobutyric acid, 4 parts of jade are taken
Rice bran oil, 13 portions of corn flour, 4 parts of cotton leafs, 8 parts of cottonseed mixing, obtain feeding stimulant.
A kind of preparation method of high-performance bio insecticide, includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 1.5h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain
Pretreatment deionized water takes 7 portions of beef extracts, 25 parts of mushroom brans, 3 parts of soybean cake powders, 12 parts of peptones, 4 parts of Portugals according to the mass fraction
Grape sugar, 3 parts of sodium chloride, 0.4 part of ferric sulfate, 0.5 part of magnesium sulfate, 0.4 part of potassium dihydrogen phosphate, 0.05 part of manganese sulfate, 1000 parts it is pre-
Deionized water, pH7.5 are handled, 121 DEG C of sterilizing 20min obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 4：2：2 mixing, obtain mix bacterium agent, take Mixed Microbes
Agent is seeded to by 5% inoculum concentration in sterilizing object, and in 28 DEG C of fermented and cultured 30h, centrifugation takes precipitation drying, obtains dried object, spare；
（3）Take methyl polyethylene glycol in mass ratio 6：2 are added succinic anhydride, add the N, N- of methyl polyethylene glycol quality 15%
Dimethylformamide is stirred 2.5h in 93 DEG C, is cooled to room temperature, obtains cooling thing, takes cooling thing in mass ratio 9：3 are added second
Ether, 35min is stood in 3 DEG C, and filtering takes filter residue in mass ratio 2：13：16 are added n-hydroxysuccinimide, dicyclohexyl carbon two
Imines is stirred 35min in 28 DEG C, adds the n,N-Dimethylformamide of filter residue quality 25%, is stirred 21h, mistake
Filter, takes filtrate in mass ratio 1：4 are added ether, take precipitation a；
（4）Take step（2）Spare dried object in mass ratio 1：13 are added the borate buffer mixing of a concentration of 0.1mol/L, then
The precipitation a of 6 times of dried object quality is added, keeps the temperature 1.5h in 34 DEG C, obtains mixed liquor, according to the mass fraction, take 40 parts of mixed liquors, 5
Part sour additive, 13 parts of feeding stimulants, 0.6 part Congo red, 10 parts of sodium alginates, 35 parts of water, and 1.5h is stirred in 35 DEG C,
Obtain high-performance bio insecticide.
Bacillus thuringiensis, does not strangle bacterium at Aschersonia：In 4 DEG C of laboratory slant preservations.
Mushroom bran：The cultivation fertilizer after needle mushroom is cultivated, wheat bran, cotton seed hulls, sawdust, quick lime are mainly contained, white lime crushes
40 mesh sieve is crossed, sieving particle is collected, it is dry to get mushroom bran.
Sour additive：Take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 3：5：1 mixing is to get sour additive.
Feeding stimulant：According to the mass fraction, 6 parts of honey, 0.8 part of l-Alanine, 0.7 part of r- aminobutyric acid, 5 parts of jade are taken
Rice bran oil, 15 portions of corn flour, 5 parts of cotton leafs, 9 parts of cottonseed mixing, obtain feeding stimulant.
A kind of preparation method of high-performance bio insecticide, includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 2h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain pre-
Processing deionized water takes 8 portions of beef extracts, 30 parts of mushroom brans, 4 parts of soybean cake powders, 13 parts of peptones, 5 portions of grapes according to the mass fraction
Sugar, 4 parts of sodium chloride, 0.5 part of ferric sulfate, 0.6 part of magnesium sulfate, 0.5 part of potassium dihydrogen phosphate, 0.06 part of manganese sulfate, 1000 parts of pre- places
Deionized water, pH7.5 are managed, 121 DEG C of sterilizing 20min obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 5：3：3 mixing, obtain mix bacterium agent, take Mixed Microbes
Agent is seeded to by 6% inoculum concentration in sterilizing object, and in 30 DEG C of fermented and cultured 36h, centrifugation takes precipitation drying, obtains dried object, spare；
（3）Take methyl polyethylene glycol in mass ratio 7：3 are added succinic anhydride, add the N, N- of methyl polyethylene glycol quality 20%
Dimethylformamide is stirred 3h in 95 DEG C, is cooled to room temperature, obtains cooling thing, takes cooling thing in mass ratio 10：4 are added second
Ether, 40min is stood in 4 DEG C, and filtering takes filter residue in mass ratio 3：15：18 are added n-hydroxysuccinimide, dicyclohexyl carbon two
Imines is stirred 40min in 30 DEG C, adds the n,N-Dimethylformamide of filter residue quality 30%, is stirred for 24 hours, mistake
Filter, takes filtrate in mass ratio 1：5 are added ether, take precipitation a；
（4）Take step（2）Spare dried object in mass ratio 1：15 are added the borate buffer mixing of a concentration of 0.1mol/L, then
The precipitation a of 7 times of dried object quality is added, keeps the temperature 2h in 38 DEG C, obtains mixed liquor, according to the mass fraction, take 50 portions of mixed liquors, 6 parts
Sour additive, 15 parts of feeding stimulants, 0.7 part Congo red, 12 parts of sodium alginates, 40 parts of water, is stirred 2h in 40 DEG C, obtains high
Imitate biological disinfestant.
Comparative example：The insecticide of certain brand production in the market.
1,2,3 sample of embodiment and comparative example of above-mentioned preparation method are subjected to effect detection, take four ventilations respectively
Then vegetation separate space is put into the mosquito of identical quantity into four spaces, equivalent is added respectively into four spaces
Insecticide is put into the mosquito of quantity identical as first time every 1h, observes the survival rate of mosquito in each space, testing result note
Record table 1：
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| For the first time put borer population amount/only | 100 | 100 | 100 | 100 |
| Borer population amount before worm/only is put after 1h for the second time | 15 | 10 | 13 | 56 |
| Borer population amount before worm/only is put after 2h for the third time | 25 | 26 | 20 | 80 |
| Mosquito survival rate/% | 3 | 5 | 3 | 40 |
| Control effect/% | 96 | 95 | 97 | 50 |
In conclusion as shown in Table 1, insecticide of the invention has preferable lasting medicine, and control effect is good, deinsectization
Efficiency is higher, has larger development prospect.
Claims (3)
1. a kind of preparation method of biological high-efficiency insecticide, which is characterized in that the preparation method includes the following steps：
（1）It removes ionized water to be placed in magnetic field generator, pre-processes 1 ~ 2h in current strength 0.2A, magnetic field intensity 14.4Gs, obtain
Pretreatment deionized water takes 5 ~ 8 portions of beef extracts, 20 ~ 30 parts of mushroom brans, 2 ~ 4 parts of soybean cake powders, 10 ~ 13 portions of eggs according to the mass fraction
White peptone, 3 ~ 5 parts of glucose, 2 ~ 4 parts of sodium chloride, 0.3 ~ 0.5 part of ferric sulfate, 0.3 ~ 0.6 part of magnesium sulfate, 0.3 ~ 0.5 part of di(2-ethylhexyl)phosphate
Hydrogen potassium, 0.03 ~ 0.06 part of manganese sulfate, 1000 parts of pretreatment deionized water mixing, sterilizing obtain sterilizing object；
（2）It takes bacillus thuringiensis, Aschersonia, strangle bacterium in mass ratio 3 ~ 5：1~3：1 ~ 3 mixing, obtains mix bacterium agent, takes
Mix bacterium agent is seeded to by 4 ~ 6% inoculum concentration in sterilizing object, and in 25 ~ 30 DEG C of fermented and cultureds, centrifugation takes precipitation drying, obtains drying
Object, it is spare；
（3）Take methyl polyethylene glycol in mass ratio 4 ~ 7：1 ~ 3 is added succinic anhydride, adds methyl polyethylene glycol quality 10 ~ 20%
N,N-Dimethylformamide, be stirred, be cooled to room temperature in 90 ~ 95 DEG C, obtain cooling thing, take cooling thing in mass ratio 8 ~
10：1 ~ 4 is added ether, and in 1 ~ 4 DEG C of standing, filtering takes filter residue in mass ratio 1 ~ 3：10~15：14 ~ 18 are added N- hydroxysuccinimidyl acyls
Imines, dicyclohexylcarbodiimide are stirred in 25 ~ 30 DEG C, add the N of filter residue quality 20 ~ 30%, N- dimethyl formyls
Amine is stirred, and filtering takes filtrate in mass ratio 1：3 ~ 5 are added ether, take precipitation a；
（4）Take step（2）Spare dried object in mass ratio 1：10 ~ 15 are added borate buffer mixing, add dry matter
4 ~ 7 times of precipitation a of amount keeps the temperature 1 ~ 2h in 30 ~ 38 DEG C, obtains mixed liquor, according to the mass fraction, take 30 ~ 50 portions of mixed liquors, 3 ~ 6 parts
Sour additive, 10 ~ 15 parts of feeding stimulants, 0.4 ~ 0.7 part Congo red, 8 ~ 12 parts of sodium alginates, 30 ~ 40 parts of water, in 30 ~ 40 DEG C
It is stirred, obtains high-performance bio insecticide.
2. the preparation method of high-performance bio insecticide according to claim 1, which is characterized in that the step（4）Middle acid
Additive is to take L- canavanine, cinnamic acid, P- coumaric acids in mass ratio 1 ~ 3：2~5：1 mixing is to get sour additive.
3. the preparation method of high-performance bio insecticide according to claim 1, which is characterized in that the step（4）In take
It is according to the mass fraction, to take 3 ~ 6 parts of honey, 0.3 ~ 0.8 part of l-Alanine, 0.4 ~ 0.7 part of r- aminobutyric acids, 2 ~ 5 to eat stimulant
Part corn oil, 10 ~ 15 portions of corn flour, 2 ~ 5 parts of cotton leafs, 6 ~ 9 parts of cottonseeds mixing, obtain feeding stimulant.
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