CN108524372A - A kind of composition and its application in preparing the cosmetics with safeguard function - Google Patents
A kind of composition and its application in preparing the cosmetics with safeguard function Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
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Abstract
A kind of application the present invention relates to cosmetic technical field more particularly to composition and its in preparing the cosmetics with safeguard function.The present invention is by Honegsukle flower P.E, Flos Buddlejae extract, peony extract is compounded, resulting composition is mixed with components such as glycerine, the Essence of formation can have the function of removing free radical, inhibit NO inflammatory Cytokines Expressions, and, the Essence can also inhibit the activity of tyrosinase, to inhibit the generation of melanin, to have the function of whitening.Experiment shows that Essence of the invention, up to 53.21%~82.58%, reduces NO burst sizes 9.37%~14.10% to free radical scavenging activity, is 75.34%~78.83% to inhibitory activity against tyrosinase.
Description
Technical field
The present invention relates to cosmetic technical field more particularly to a kind of composition and its preparing the change with safeguard function
Application in cosmetic.
Background technology
Loving beauty is part of human nature, and beautiful pursuit is continuously improved with the progress of civilization by the mankind.Chinese beauty culture source
Remote stream length, can trace back to the Neolithic Age earliest, be the indispensable component part of human lives.The beauty culture of the Chinese nation
In, in addition to liking natural beauty, also like that beautification of the mind and appearance are beautiful.Traditional Chinese medicine beautifying is that based on TCM theory, have with some
The method and prescription of tcm characteristic are means, by adjusting viscera function, improve blood circulation, beautiful to realize cleaning face
Change the purpose of skin.Classical ancient books is consulted, many ancient times beauties are familiar with the road of beauty treatment, commonly using the production with moistening face
Product.
With the raising of social progress and living standards of the people, the consciousness that people pursue health is increasingly enhanced, due to planting
Object active constituent not only Small side effects but also effect is preferable, the beauty product based on active components of plants is increasingly consumed
The favor of person.It is detached from natural plants or Chinese medicine, extraction active constituent makes cosmetics and skincare product and increasingly causes attracting attention for the world.
Flower is the organ of multiplication of plant, she is gorgeous colorful, in different poses and with different expressions, fragrant, everybody likes.It has attractive appearance such as flower, is
The Chinese nation is to a kind of beautiful expression.Chinese medicine thinks that fresh flower light nature easily reaches skin bottom, promotes blood circulation, new old
Metabolism.Modern pharmacology research provides scientific basis for the beauty and skin care of flowers：Contain various alkaloids, ester in flowers
Class, organic acid, vitamin and trace element etc., these substances have preferable skin care, beauty treatment, beautification function.As people are strong
Health living awareness enhances, and people are increasingly keen to the beauty Huang hair of plant cosmetics and back to nature, thus it is believed that flower
Grass beauty, extraction all sorts of flowers are succinct, to spend beauty treatment, are well worth doing.
Currently, chaotic by the kind of the cosmetics of raw material of plant in the market, quality is very different.Analyze reason, a side
That face is that other businessman pursues is benefit, passes a fake product off as a genuine one, is substituted the bad for the good with bad.More important reason, which is the formula of cosmetics, not to be had
By further verifying, synergistic effect cannot be played between each component well, therefore, how to use plant material,
Especially using flower as raw material, the cosmetics with good function are developed, the cosmetic result of cosmetics is improved, is still current research
Hot spot.
Invention content
In view of this, the technical problem to be solved in the present invention is to provide a kind of composition and its has protection work(preparing
Can cosmetics in application, the composition can anti-oxidant, anti-inflammatory and whitening, to play good protection work to skin
With.
Composition provided by the invention is grouped as by the group of following mass fraction：
0.2 part~1 part of Flos Buddlejae extract；
0.2 part~1 part of peony extract；
0.5 part~1 part of Honegsukle flower P.E.
In some embodiments, the composition is grouped as by the group of following mass fraction：
0.3 part of Flos Buddlejae extract；
0.3 part of peony extract；
0.5 part of Honegsukle flower P.E.
In other embodiments, the composition is grouped as by the group of following mass fraction：
0.2 part of Flos Buddlejae extract；
0.2 part of peony extract；
0.5 part of Honegsukle flower P.E.
In other embodiments, the composition is grouped as by the group of following mass fraction：
1 part of Flos Buddlejae extract；
1 part of peony extract；
1 part of Honegsukle flower P.E.
This patent uses honeysuckle, butterflybush flower, and peony is " being protected with flower " prescription, carries out the protection of skin light, supplement
Amino acid and vitamin, mineral trace element, diminish inflammation, and enhance immunocompetence, accelerate blood circulation, boost metabolism
Dimension promotes skin natural protection ability.
The present invention also provides a kind of Essences, by glycerine, butanediol, propylene glycol, beta glucan, oligomerization hyaluronic acid
Sodium, trehalose, niacinamide, inositol, carnosine, ursin and composition of the present invention composition.
The present invention compounds Flos Buddlejae extract, peony extract, Honegsukle flower P.E, and resulting composition is used for
The Essence of cosmetics is prepared, experiment shows that the Essence, can phase to being 53.21%~82.58% to free radical scavenging activity
The damage generated to cell to efficiently removing free radical there is preferable repair to defend the intact of skin barrier.The essence
The burst size that liquid energy effectively reduces inflammatory factor ON is 9.37%~14.10%, and for infrared ray, UV-induced inflammation has
Certain repair.Also, the Essence is 75.34%~78.83% to inhibitory activity against tyrosinase, for ultraviolet light
Cause melanin has good inhibiting effect, and melanin generation and pigmentation can be prevented to have protective action.
In the Essence, the components such as glycerine, butanediol, propylene glycol can coordinate composition provided by the invention, further
Improve the burst size of composition removed free radical, inhibit inflammatory factor, the effect inhibited tyrosinase activity.
Specifically, Essence provided by the invention is grouped as by the group of following mass fraction：
In some embodiments, Essence provided by the invention is grouped as by the group of following mass fraction：
In some embodiments, Essence provided by the invention is grouped as by the group of following mass fraction：
In some embodiments, Essence provided by the invention is grouped as by the group of following mass fraction：
Further include water, preservative and thickener in Essence provided by the invention.
The preservative is PHL；The thickener is xanthans.
In the Essence, the mass fraction of water is 84.61%~86.71%.
Wherein, the mass fraction of the preservative is 1%, and the mass fraction of the thickener is 0.15%.
Application of the Essence of the present invention in preparing oxidation resistant cosmetics.
The antioxygen turns to removing free radical.
Application of the Essence of the present invention in preparing anti-inflammatory cosmetics.
The anti-inflammatory expression for inhibition inflammatory factor.
Application of the Essence of the present invention in preparing the cosmetics inhibited tyrosinase activity.
The preparation method of Essence of the present invention includes：
75 DEG C~80 DEG C, by glycerine, butanediol, propylene glycol, oligomerization Sodium Hyaluronate, trehalose, β-glucan, black bearberry
Glycosides, niacinamide, inositol, thickener and water mixing, 500r/min~600r/min stir 30min；
55 DEG C~60 DEG C are cooled to, sequentially adds carnosine, Flos Buddlejae extract, peony extract, Honegsukle flower P.E,
300r/min~350r/min stirs 15min；
45 DEG C~50 DEG C are cooled to, preservative is added, 150r/min~200r/min stirs 10min；It is cooled to room temperature.
The room temperature is 18 DEG C~30 DEG C.
The present invention also provides a kind of anti-oxidant, anti-inflammatory and/or whitening cosmetics, including essence of the present invention
Liquid.
Cosmetics of the present invention include：Mask or eye sticker patch.
The cosmetics are facial mask, then wherein also include mask matrix.
The present invention is compounded Honegsukle flower P.E, Flos Buddlejae extract, peony extract, resulting composition with it is sweet
The components such as oil are mixed, and the Essence of formation can have the function of removing free radical, inhibit NO inflammatory Cytokines Expressions, and
And the Essence can also inhibit the activity of tyrosinase, to inhibit the generation of melanin, to have the function of whitening.
Experiment shows that Essence of the invention, up to 53.21%~82.58%, reduces NO burst sizes 9.37% to free radical scavenging activity
~14.10%, it is 75.34%~78.83% to inhibitory activity against tyrosinase.
Description of the drawings
Fig. 1 shows influence of the different samples to NO burst sizes；
Fig. 2 shows the removing free radical effect of different samples；
Fig. 3 shows inhibiting rate of the different samples to tyrosinase activity；
Specific implementation mode
A kind of application the present invention provides composition and its in preparing the cosmetics with safeguard function, this field skill
Art personnel can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that all similar replacements and
Change apparent to those skilled in the art, they are considered as being included in the present invention.The present invention method and
Using being described by preferred embodiment, related personnel can obviously not depart from the content of present invention, spirit and scope
It is interior that methods herein and application are modified or are suitably changed and combined, to realize and apply the technology of the present invention.
The raw material that the present invention uses is all common commercially available product, can all be bought in market.
Honeysuckle also known as honeysuckle flowers are half evergreen voluble shrub of Caprifoliaceae.Honegsukle flower P.E provided by the invention is main
Containing chlorogenic acid, isochlorogenic acid, cyanidenon and saponin(e, it can effectively inhibit golden yellow Portugal coccus, Escherichia coli, Pseudomonas aeruginosa
Deng, and 1L-4 levels can be promoted, neurocalcin is activated, macrophage function is enhanced, protects collagen.The present invention uses
Hunan Longhui spends the green phase of the precious jade area rivers little Sha honeysuckle three, i.e., prepares honeysuckle flower extract using green flower bud, and plucking time is upper
Noon 9:00 to 11：00；The preparation of extract uses water extraction.After testing, the extract Content of Chlorogenic Acid and isopropyl chlorogenic acid activity
Constituent content is higher, has excellent antibacterial, anti-inflammatory and antioxygenic property.
Butterflybush flower, alias butterfly wood, the main component of Flos Buddlejae extract is verbascoside, echinacoside.The present invention with
The flower of butterflybush flower is raw material, and the preparation of extract uses water extraction.The extract can resist infrared light comprehensively, ultraviolet light,
Lipid oxidation caused by blue light, DNA Damage, extracellular matrix are disintegrated, and pigmentation is prevented, and structure 360 is subsisted photoprotection；
Peony category Ranunculaceae Paeonia shrub is rich in multiple nutritional components, including polyphenols, egg in fresh peony flower
The trace element of white matter, amino acid and needed by human body.Using the petal of peony as raw material, the preparation of extract uses the present invention
The main component of water extraction, extract is protein, vitamin V b2, carotenoid, trace element, which passes through offer
Abundant protein and amino acid promote body glycometabolism and protein metabolism, and it is anti-to participate in a variety of oxidizing ferment by vitamin V B2
It answers, angling hyperplasia pre- anti-drying by carotenoid, by trace element, a variety of coenzyme is promoted to synthesize.
Glycerine is also known as glycerine, has water sorption, is used as moisturizer.
Butanediol is one kind of polyalcohol, is often used as moisturizer or solvent in cosmetics, the fourth that the present invention uses
Glycol is 1,3 butanediols；
Propylene glycol has the function of moisturizing and antibacterial, and the propylene glycol that the present invention uses is 1,2-PD；
Beta glucan is also known as dextran, mainly by D- glucopyanosyls with α, 1 → 6 key connection, branch point has 1 → 2,
1 → 3,1 → 4 connection.The molecular weight for the beta glucan that the present invention uses is 60,000~500,000.
Oligomerization Sodium Hyaluronate is the extremely low hyaluronan molecule of the molecular weight that is prepared by hyaluronic acid degradation, has skin
The function of moisturizing.The molecular weight for the oligomerization Sodium Hyaluronate that the present invention uses is 10000.
Trehalose is also known as Radix Rhapontici seu Radix Echinopsis sugar, gill fungus sugar etc., has the function of cytoprotection.
Niacinamide, also known as niacinamide are the amide compounds of niacin, and nutritive additive is used as in cosmetics.
Inositol also known as inose, inositol or inositol (inose), participate in internal metabolic activity.
Carnosine (L-Carnosine), scientific name β-alanyl-L-histidin, be by be one kind by Beta-alanine and L- group ammonia
The dipeptides of sour two kinds of amino acid composition, can remove the aliphatic acid excessive oxidation for making cell membrane during oxidative stress and be formed in fact
Active oxygen radical (ROS) and alpha-beta unsaturated aldehyde.
Ursin also known as Arbutin can reduce Skin pigmentation, dispelling stain and freckle, while also have and sterilize, disappear
Scorching effect.It is extractable from plant, can also be synthesized by artificial chemistry.
With reference to embodiment, the present invention is further explained：
Examples 1 to 3
Formula such as table 1
1 Examples 1 to 3 formula of table
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Glycerine | 3g | 3g | 3g |
| Butanediol | 5g | 5g | 5g |
| Propylene glycol | 2g | 2g | 2g |
| Beta glucan | 0.1g | 0.1g | 0.1gg |
| Oligomerization Sodium Hyaluronate | 0.01g | 0.01g | 0.01g |
| Trehalose | 0.01g | 0.01g | 0.01g |
| Niacinamide | 1g | 1g | 1g |
| Inositol | 0.1g | 0.1g | 0.1g |
| Carnosine | 0.01g | 0.01g | 0.01g |
| Ursin | 0.01g | 0.01g | 0.01g |
| Flos Buddlejae extract | 0.2g | 0.3g | 1g |
| Peony extract | 0.2g | 0.3g | 1g |
| Honegsukle flower P.E | 0.5g | 0.5g | 1g |
| PHL | 1g | 1g | 1g |
| Xanthans | 0.15g | 0.15g | 0.15g |
| Water | 86.71g | 86.51g | 84.61g |
1) according to formulation ratio weigh glycerine, butanediol, propylene glycol, oligomerization Sodium Hyaluronate, trehalose, beta glucan,
Ursin, niacinamide, inositol, xanthans and deionized water raise the temperature to 75~80 DEG C, with 500~600r/min, stirring
30min, until component all dissolves；
2) temperature is reduced to 55~60 DEG C, sequentially adds carnosine, Flos Buddlejae extract, peony extract, honeysuckle
Extract stirs 15min with 300~350r/min, until all uniformly mixing；
3) temperature is reduced by 45~50 DEG C, adds preservative PHL, 10min is stirred with 150~200r/min, is cooled to room
Temperature.
Comparative example 1~3
Formula such as table 2
2 comparative example 1~3 of table is formulated
| Component | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 |
| Glycerine | 3g | 3g | 3g | 3g |
| Butanediol | 5g | 5g | 5g | 5g |
| Propylene glycol | 2g | 2g | 2g | 2g |
| Beta glucan | 0.1g | 0.1g | 0.1gg | 0.1g |
| Oligomerization Sodium Hyaluronate | 0.01g | 0.01g | 0.01g | 0.01g |
| Trehalose | 0.01g | 0.01g | 0.01g | 0.01g |
| Niacinamide | 1g | 1g | 1g | 1g |
| Inositol | 0.1g | 0.1g | 0.1g | 0.1g |
| Carnosine | 0.01g | 0.01g | 0.01g | 0.01g |
| Ursin | 0.01g | 0.01g | 0.01g | 0.01g |
| Flos Buddlejae extract | -- | 3g | 1.5g | 0.25g |
| Peony extract | -- | -- | 1.5g | 0.25g |
| Honegsukle flower P.E | -- | -- | -- | 2.5g |
| PHL | 1g | 1g | 1g | 1g |
| Xanthans | 0.15g | 0.15g | 0.15g | 0.15g |
| Water | 87.61g | 84.61g | 84.61g | 84.61g |
1) according to formulation ratio weigh glycerine, butanediol, propylene glycol, oligomerization Sodium Hyaluronate, trehalose, beta glucan,
Ursin, niacinamide, inositol, xanthans and deionized water raise the temperature to 75~80 DEG C, with 500~600r/min, stirring
30min, until component all dissolves；
2) temperature is reduced to 55~60 DEG C, sequentially adds carnosine, Flos Buddlejae extract, peony extract, honeysuckle
Extract stirs 15min with 300~350r/min, until all uniformly mixing；
3) temperature is reduced by 45~50 DEG C, adds preservative PHL, 10min is stirred with 150~200r/min, is cooled to room
Temperature.
Effect is evaluated and tested
1DPPH method free radical scavenging abilities measure
1.1 tests prepare
1.1.1DPPH test fluid configures
Precise 1mgDPPH, 95% ethyl alcohol constant volume and 50ml volumetric flasks, ultrasonic 3min are allowed to be completely dissolved.Measure 1ml
Solution measures absorbance A at 519nm, and A values can be used between 0.6~0.8.
1.1.2Vc standard curve determination
Vc is configured to five various concentrations of 0.1,0.2,0.3,0.4,0.5mmol/L with deionized water.It is added in small test tube
2ml DPPH solution, 100 μ lVc solution, 400 μ l95% ethyl alcohol, mixing shake up, and stand 5min, survey A values (519nm).Blank pair
According to for 100 μ l deionized waters.Draw concentration-absorbance standard curve.
Vc clearance rates=(A0-AX)/A0
A0：Deionized water blank control, Ax：Vc absorbances
1.1.3 sample measures
100 μ l of sample cosmetics of comparative example 1~4 (Examples 1 to 3) solution, are detected according to step in (2), to avoid
Solution colour influences, and setting is not added with DPPH control groups, and it is that sample absorbance subtracts control group absorbance to calculate absorbance.Often survey one
A used in amounts will survey three panel datas.And after three panel datas of one dosage of every survey, an A0 will be resurveyed.It surveys
Obtain sample absorbance, clearance rate, comparison Vc oxidation resistances and sample total antioxidant capacity such as Fig. 1.
The results show that each embodiment all has free radical a good scavenging effect, clearance rate is 53.21%~
82.58%, the damage that free radical generates cell can be efficiently removed, there is preferable repair to prevent the intact of skin barrier
It is imperial.Through statistical analysis, the significant effect of Examples 1 to 3 is better than each control sample (p<0.05), and the effect of embodiment 3 is also shown
The sample better than embodiment 1 and embodiment 2 of work property, p<0.05.It these results suggest that, the effect of composition provided by the present application
Fruit, which is significantly better than, lacks extracting section object or extract each group distribution ratio improperly sample.
2LPS induces RAW264.7 cells to discharge NO inhibiting effect
2.1 experimental principle：
LPS (lipopolysaccharides)：
Positioned at the lipopolysaccharides substance of gram-negative bacterial cell wall outermost one layer thicker (8-10nm), by class
Fat A, core polysaccharide and 3 part of O- specific side chains composition.Lipopolysaccharides is endotoxin and important group specific antigen (O antigens).Fat
Polysaccharide is made of three parts.Lipid A (Lipid A) is the glycolipid for constituting activity of endotoxin, with covalent bonding junction to heteroglycan chain,
There are two parts：First, core polysaccharide, is constant in related strain；Another O specificity chain (O-specific chain) is
Alterable height.The lipopolysaccharides of Escherichia coli is the i.e. polyclonal work of common B cell mitogeneic factor in Experimental immunization
Change the factor (polyclonal activator).This experiment generates allergic reaction, release using LPS induction RAW264.7 cells
NO (nitric oxide).
2.2Griess assay-NO content test methods
NO is easily oxidized to NO in vivo or in aqueous solution2, in acid condition, NO and diazol sulfanilamide (SN) generation diazonium are anti-
It answers, and generates diazonium compound, coupled reaction further occurs with naphthylethenyl diamines for the latter, and the product which generates is dense
Degree has linear relationship with NO concentration, there is maximum absorption band at 540nm.
2.3 experiment materials and method：
2.3.1 material：DMEM, FBS, LPS, MTT, Griess reagent
2.3.2 experimental method：
264.7 cell culture of RAW：RAW264.7 cells are drawn into DMEM training bases with liquid-transfering gun and dispel cell dispersion, are utilized
Hemacytometer counts cell, is then diluted to a concentration of 5 × 104cells/ml using DMEM come diluting cells.
Cell solution after dilution is inoculated into 96well respectively, and each hole is 100ul, i.e. 5 × 103 cells/well.
At 37 DEG C, 5%CO2Incubator in cultivate 24 hours.
Sample to be tested and blank sample prepare：The sample to be tested cosmetics of comparative example 1~4 (Examples 1 to 3) is trained with DMEM
Base dilutes, and the concentration after dilution is respectively：1% (concentration is tested by the MTT of front, and result is nontoxic)
Title Control Sample
The sample composition LPS 10ppm LPS 10ppm+1%sample cosmetics of comparative example 1~4 (Examples 1 to 3)
After cell culture 24 hours, the whether complete adherent growth of cell is observed, it, will if the complete adherent growth of cell
Original training base removes, and is washed with DPBS.
After DPBS is removed, it is separately added into the ready sample in front.The solubility of sample is according to the result of toxotest
Select safe concentration 1% (20ul/well).
After sample is added, 37 DEG C are put into, 5%CO2Incubator in cultivate 20 hours.
After 20 hours, on the culture medium to new 96Well that pipettes 50 μ l, 1% Griess of same volume is added
Reagent after being sufficiently mixed, reacts 10 minutes in dark place.
Utilize the absorption photometric value of ELISAreader test samples at 540nm.
NO inhibiting effect
NO Inhibition%=((" OD " _ Control- " OD " _ Sample)/" OD " _ Control) * 100%
Wherein：The absorption photometric value of " OD " _ Control- blank samples
The absorption photometric value of " OD " _ Sample- samples
Experimental result such as Fig. 2：The results show that each embodiment can all effectively reduce the release of inflammatory factor ON, the amount of reduction
It is 9.37%~14.10%, for infrared ray, UV-induced inflammation has certain repair.It is real through statistical analysis
The significant effect for applying example 1~3 is better than each control sample (p<0.05), and the effect of embodiment 3 also conspicuousness be better than 1 He of embodiment
The sample of embodiment 2, p<0.05.It these results suggest that, the significant effect of composition provided by the present application is carried better than part is lacked
Take object or extract each group distribution ratio improperly sample.
3 tyrosinase activity inhibitions are tested
3.1 reagent：0.175mol/L PBS (pH 6.8), 1.5mmol/L tyrosine, 2000unit/mL tyrosinases
Laboratory sample prepares：The sample cosmetics of comparative example 1~4 (Examples 1 to 3) is diluted using PBS, it is dilute
Concentration after releasing is respectively 5%, 10%, 50%
3.2 test method：
1) 4 skies are chosen on 96 well plate, are added according to the additive amount of each substance in lower table
2) concussion is positioned in 37 DEG C of incubator after being sufficiently mixed and reacts
3) absorbance (Ab, Absorbance) is tested at 490nm wavelength using microplate reader after 25 minutes
4.) inhibition of tyrosinase activity is calculated using formula below：
3.3 experimental results such as Fig. 3, the results showed that：The cosmetics of each embodiment all have tyrosinase activity good suppression
It makes and uses, inhibiting rate is 75.34%~78.83%, it is seen then that the cosmetics can be directed to the generation that ultraviolet light causes melanin
There is good inhibiting effect, can prevent melanin generation and pigmentation that there is protective action.Through statistical analysis, embodiment 1
~3 effect be better than each control sample, and the effect of embodiment 3 also conspicuousness be better than Examples 1 to 2 and each comparative example,
p<0.05.It these results suggest that, the significant effect of composition provided by the present application is better than lacking extracting section object or extract
Each group distribution ratio improperly sample.
It the above is only the preferred embodiment of the present invention, it is noted that those skilled in the art are come
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of composition, which is characterized in that be grouped as by the group of following mass fraction：
0.2 part~1 part of Flos Buddlejae extract；
0.2 part~1 part of peony extract；
0.5 part~1 part of Honegsukle flower P.E.
2. composition according to claim 1, which is characterized in that be grouped as by the group of following mass fraction：
0.3 part of Flos Buddlejae extract；
0.3 part of peony extract；
0.5 part of Honegsukle flower P.E.
3. a kind of Essence, which is characterized in that by glycerine, butanediol, propylene glycol, beta glucan, oligomerization Sodium Hyaluronate, seaweed
Sugar, niacinamide, inositol, carnosine, ursin and composition as claimed in claim 1 or 2 composition.
4. Essence according to claim 3, which is characterized in that be grouped as by the group of following mass fraction：
5. Essence according to claim 3 or 4, which is characterized in that further include water, preservative and thickener.
6. Essence according to claim 5, which is characterized in that the mass fraction of the water be 84.61%~
86.71%.
7. application of any one of claim 3~6 Essence in preparing anti-oxidant, anti-inflammatory and/or whitening cosmetics.
8. the preparation method of any one of claim 3~6 Essence, which is characterized in that including：
75 DEG C~80 DEG C, by glycerine, butanediol, propylene glycol, oligomerization Sodium Hyaluronate, trehalose, beta glucan, ursin, cigarette
Amide, inositol, thickener and water mixing, 500r/min~600r/min stir 30min；
55 DEG C~60 DEG C are cooled to, sequentially adds carnosine, Flos Buddlejae extract, peony extract, Honegsukle flower P.E,
300r/min~350r/min stirs 15min；
45 DEG C~50 DEG C are cooled to, preservative is added, 150r/min~200r/min stirs 10min；It is cooled to room temperature.
9. a kind of anti-oxidant, anti-inflammatory and/or whitening cosmetics, which is characterized in that described in any one of claim 3~6
Essence.
10. cosmetics according to claim 9, which is characterized in that it is facial mask, further includes mask matrix.
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| CN201810672706.0A CN108524372A (en) | 2018-06-26 | 2018-06-26 | A kind of composition and its application in preparing the cosmetics with safeguard function |
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| CN201810672706.0A CN108524372A (en) | 2018-06-26 | 2018-06-26 | A kind of composition and its application in preparing the cosmetics with safeguard function |
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| CN109806183A (en) * | 2019-04-03 | 2019-05-28 | 成都肌本演绎美丽科技有限公司 | A kind of preparation method and photoprotection application of the Essence containing beta glucan |
| CN112754971A (en) * | 2021-03-24 | 2021-05-07 | 广州环亚化妆品科技有限公司 | Traditional Chinese medicine composition and preparation method and application thereof |
| CN115054556A (en) * | 2022-07-29 | 2022-09-16 | 上海新高姿化妆品有限公司 | Composition for repairing photodamage and application thereof |
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| CN107049880A (en) * | 2017-06-16 | 2017-08-18 | 湖南御家化妆品制造有限公司 | Lightening compositions and its application |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109806183A (en) * | 2019-04-03 | 2019-05-28 | 成都肌本演绎美丽科技有限公司 | A kind of preparation method and photoprotection application of the Essence containing beta glucan |
| CN109806183B (en) * | 2019-04-03 | 2022-03-04 | 成都肌本演绎美丽科技有限公司 | Preparation method and photoprotection application of beta-glucan-containing essence |
| CN112754971A (en) * | 2021-03-24 | 2021-05-07 | 广州环亚化妆品科技有限公司 | Traditional Chinese medicine composition and preparation method and application thereof |
| CN115054556A (en) * | 2022-07-29 | 2022-09-16 | 上海新高姿化妆品有限公司 | Composition for repairing photodamage and application thereof |
| CN115054556B (en) * | 2022-07-29 | 2023-08-01 | 上海新高姿化妆品有限公司 | Composition for repairing photodamage and application thereof |
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